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Intravenous lipid emulsion as an adjuvant therapy of acute clozapine poisoning Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2021-01-05 Fatma M Elgazzar; Mona S Elgohary; Sara M Basiouny; Heba I Lashin
Introduction: Clozapine is a frequently prescribed atypical antipsychotic drug. Various case reports documented the successful recovery of acute antipsychotics toxicity in association with the administration of intralipid emulsion (ILE). Aim: This study aimed to assess the adjuvant therapeutic role of SMOF Lipid administration on the outcomes of acute clozapine poisoning. Methods: Forty patients with
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FOXM1 regulates the proliferation, apoptosis and inflammatory response of keratinocytes through the NF-κB signaling pathway Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2021-01-05 Mi Zhou; Jing Shi; Shaobo Lan; Xianjun Gong
Psoriasis is a common immune-mediated and genetic skin disease. Forkhead box M1 (FOXM1) is a member of FOX family that has been found to modulate skin disorders. However, its role in psoriasis remains unknown. Thus, we aimed to investigate the effect of FOXM1 on keratinocytes in response to tumor necrosis factor-α (TNF-α). The expression levels of FOXM1 in psoriasis tissues and normal skin tissues
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Assessment of oxidative DNA damage, oxidative stress responses and histopathological alterations in gill and liver tissues of Oncorhynchus mykiss treated with linuron Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-31 Ahmet Topal; Arzu Gergit; Mustafa Özkaraca
We investigated changes in 8-hydroxy-2-deoxyguanosine (8-OHdG) activity which is a product of oxidative DNA damage, histopathological changes and antioxidant responses in liver and gill tissues of rainbow trout, following a 21-day exposure to three different concentrations of linuron (30 µg/L, 120 µg/L and 240 µg/L). Our results indicated that linuron concentrations caused an increase in LPO levels
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Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-31 Gamze Guney Eskiler; Asuman Deveci Ozkan; Isil Ezgi Eryilmaz; Unal Egeli; Gulsah Cecener
Background: We evaluated the effect of cabazitaxel (CAB) as a third-line taxane on Toll-like receptor 4 (TLR4)-mediated signaling pathways, especially NF-κB activity, in metastatic castration-resistant prostate cancer (mCRPC) cells. Methods: CAB cytotoxicity was determined by WST-1 assay. To assess the relationship between CAB efficacy and TLR4 signaling pathways, RT-PCR, western blot and immunofluorescence
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JZL184 protects hippocampal neurons from oxygen-glucose deprivation-induced injury via activating Nrf2/ARE signaling pathway Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-30 Jing Xu; Qinyue Guo; Kang Huo; Yinxue Song; Na Li; Junkai Du
JZL184 is a selective inhibitor of monoacylglycerol lipase (MAGL) that has neuroprotective effect. However, the role of JZL184 in cerebral ischemia/reperfusion (I/R) injury and the exact mechanism have not been fully understood. This study was designed to elucidate the role of JZL184 in cerebral I/R injury induced by oxygen-glucose deprivation/reoxygenation (OGD/R) in hippocampal neurons. Hippocampal
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Sestrin2 protects against traumatic brain injury by reinforcing the activation of Nrf2 signaling Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-30 Xiaobin Liu; Min Li; Jiabao Zhu; Weidong Huang; Jinning Song
Sestrin2 (SESN2) is stress-inducible protein that confers cytoprotective effects against various noxious stimuli. Accumulating evidence has documented that SESN2 has potent anti-apoptosis and anti-oxidative stress functions. However, whether it provides neuroprotection in traumatic brain injury (TBI) models remains unexplored. The purpose of this study was to explore the regulatory effect of SESN2
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The PAD4 inhibitor GSK484 enhances the radiosensitivity of triple-negative breast cancer Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-23 Lining Wei; Xiangping Wang; Min Luo; Hongzhi Wang; Hao Chen; Changjie Huang
Triple-negative breast cancer (TNBC) accounts for approximately 10–20% of all breast cancers and is one of the leading causes of mortality among females. Radiotherapy is essential during the treatment of breast cancer. Growing evidence has indicated that peptidyl arginine deiminase-4 (PAD4) inhibitor can alleviate the development of multiple cancers, including breast cancer, through inhibiting cell
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Corrigendum Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-23
Radhiga T, Senthil S, Sundaresan A, et al. Ursolic acid modulates MMPs, collagen-I, α-SMA, and TGF-β expression in isoproterenol-induced myocardial infarction in rats. Hum Exp Toxicol 2019; 38(7): 785–793. DOI: 10.1177/0960327119842620.
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Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-20 Yuan Gao; Xinjia Zhou; Yan Zhou; Wei Zhang; Li Zhao
Chrysene, one of the basic polycyclic aromatic hydrocarbons (PAHs), has been reported to make damages to human health and living environment. Chronic obstructive pulmonary disease (COPD) is a progressive disorder with high morbidity and mortality. To investigate the role of chrysene in the development of COPD, male C57BL/6 mice were exposed to the cigarette smoke (CS) followed with the administration
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In vitro and in vivo evaluation of clastogenicity of second-line antitubercular drug loaded PLGA nanoparticles Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-20 Avaneesh Kumar Pandey; Rajendra Kumar; Nusrat Shafiq; Ritika Kondel; Shanky Garg; Harish Negi; Sunil Kumar Arora; Neelam Varma; Samir Malhotra
Sustained release nanoformulations of second line antitubercular drugs levofloxacin and ethionamide had shown promise in pharmacokinetics and acute and sub-acute toxicity studies. The present study evaluated the clastogenicity potential of the nanoformulations of these antitubercular agents. Clastogenicity was evaluated by (a) in vitro micronucleus assay (b) in vivo micronucleus assay in Swiss albino
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Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-16 Yasin Bagheri; Shadi Aghajani; Mahla Hosseinzadeh; Farid Hoshmandan; Abdollah Abdollahpour; Sepideh Zununi Vahed
Background: Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients’ outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injuries following IRI. The objectives of this study were to determine the impact of Gamma
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Effects of psoralen on hepatic bile acid transporters in rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-15 Juyang Huang; Qin Wang; Mengying Chen; Yanan Bi; Hong Shi; Kun Zhou
Fructus Psoraleae (FP), widely used in traditional medicine, is increasingly reported to cause serious hepatotoxicity in recent years. However, the main toxic constituents responsible for hepatotoxicity and the underlying mechanisms are poorly understood. In the present study, psoralen, a main and quality-control constituent of FP, was intragastrically administered to Sprague-Dawley rats at a dose
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Ephedrine attenuates cerebral ischemia/reperfusion injury in rats through NF-κB signaling pathway Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-14 Chanhong Shi; Jianhong Li; Jianwei Li
The inflammation and immune responses are critical in ischemic stroke and contribute to aggravated brain damage. Ephedrine was reported to play an important role in the control of inflammatory responses. This study was to investigate the repairing effects and potential mechanisms of ephedrine on cerebral ischemic injury in a rat model of focal cerebral ischemia. The rat model of cerebral ischemia/reperfusion
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Tanshinone ⅡA may alleviate rifampin-induced cholestasis by regulating the expression and function of NTCP Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-14 Y Yang; L Liu; M Xu; X Zhang; L Wang; Q He; M Xu; X Jiang
The Na+-taurocholate cotransporting polypeptide (NTCP) acts as the major hepatic basolateral uptake system, and plays a key role in balancing bile flow. The anti-tuberculosis drugs rifampin (RFP) can affect bile flow causing liver injury, while tanshinone IIA (TAN IIA) has the effect of protecting liver. This study aimed to investigate the effects of RFP and TAN IIA on the NTCP expression and activity
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Effects of Dicer1 targeted by EBV-miR-BART6-5p on biological properties and radiosensitivity of nasopharyngeal carcinoma Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-11 Jing Tang; Zhao-Yang Liu; Yi Tang; Yan Wang
Objective: To discuss the effects of Epstein-Barr virus (EBV)-encoded BamHI A rightward transcript (BART) microRNA (miR-BART6-5p) by targeting Dicer1 on biological properties and radiosensitivity of nasopharyngeal carcinoma (NPC). Methods: NPC patients (n = 96) treated with radiotherapy were collected from Jan 2010 to Jan 2011. Real-time quantitative PCR (qRT-PCR) and western blot were carried out
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LncRNA PART1 promotes lung squamous cell carcinoma progression via miR-185-5p/Six1 axis Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-10 Y Cao; R Zhang; X Luo; Y Yang
Dysregulation of the long non-coding RNA prostate androgen regulated transcript 1 (lncRNA PART1) is involved in the tumorigenesis of various cancers. However, little is known about its function and molecular mechanism in the development of lung squamous cell carcinoma (LSCC). In this study, we examined the expression of PART1 in LSCC clinical tissue samples and cell lines, and gain- and loss-of-function
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Investigation of the plasma copeptin level in cases with childhood migraine Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-09 Halil Kazanasmaz; Mustafa Calik; Huseyin Gümüş; Ismail Koyuncu; Özlem Kazanasmaz
Copeptin is a hypothalamic stress hormone that is synthesized in the hypothalamus together with Arginine-vasopressin and circulated from the neurohypophysis in equimolar amounts and can indicate the individual stress level. The aim of this study was to investigate the plasma copeptin level for childhood migraine headache. In this study, total oxidant status (TOS); total antioxidant status (TAS); oxidative
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Profile of incidental exposures to e-cigarette liquids in Europe, 2018–2019 Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-12-04 Constantine I Vardavas; Charis Girvalaki; Satomi Odani; Katerina Nikitara; Irma de Vries; Antoinette van Riel; Iris van Sommeren-de Potter; Johanna Nordmark Grass; Maria Caterina Grassi; Szilvia Deim; Andrea Balázs; Szabolcs Fosztó; Helmut Schiel; Tara Arif; Anna-Kaisa Eronen; Antonio Alonso Alonso; José Luis Conejo Menor; Rosa Martinez Arrieta; Željka Babić; Rajka Turk; Alexander I Vardavas; Aristidis
Background: Following the implementation of the European Union Tobacco Product Directive (EU TPD) regulations on e-cigarette products in 2016, we assessed the current profile of e-cigarette liquid exposure incidents and their associated health outcomes. Methods: De-identified data were received from poison centers in eight EU Member States (Sweden, the Netherlands, Italy, Hungary Austria, Finland,
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Cannabinoid CB1 receptor mediates METH-induced electrophysiological and morphological alterations in cerebellum Purkinje cells Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-28 Effat Ramshini; Majid Sheykhzade; Shahriar Dabiri; Mohammad Shabani
Our previous studies on cannabinoid type1 receptor (CB1R) activation on Methamphetamine (METH)-induced neurodegeneration and locomotion impairments in male rats suggest an interaction between CB1Rs and METH. However, the role of these receptors in METH-neurotoxicity has not been fully identified. Therefore, the purpose of the present study is to investigate the involvement of CB1Rs in these effects
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Anti-inflammatory and anti-apoptotic effect of zingiberene on isoproterenol-induced myocardial infarction in experimental animals Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-26 Jianwei Li; Radhiga Thangaiyan; Kanimozhi Govindasamy; Jianxia Wei
This study aimed to investigate the antihyperlipidemic and anti-inflammatory effect of zingiberene (ZBN) on isoproterenol-(ISO) induced myocardial infarction in rats. ZBN (10 mg/kg b.wt.) was orally administered to rats for 21 days and ISO (85 mg/kg b.wt.) was subcutaneously injected into the rats at 24 h intervals for the last 2 consecutive days. We observed increased serum creatine kinase, creatine
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Subacute melamine exposure disrupts task-based hippocampal information flow via inhibiting the subunits 2 and 3 of AMPA glutamate receptors expression Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-26 Wei Sun; Xiaoliang Li; Dongxin Tang; Yuanhua Wu; Lei An
Although melamine exposure induces cognitive deficits and dysfunctional neurotransmission in hippocampal Cornus Ammonis (CA) 1 region of rats, it is unclear whether the neural function, such as neural oscillations between hippocampal CA3–CA1 pathway and postsynaptic receptors involves in these effects. The levels of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) subunit
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LncRNA MIAT regulated by selenium and T-2 toxin increases NF-κB-p65 activation, promoting the progress of Kashin-Beck Disease Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-25 Min Shi; Ying He; Ying Zhang; Xiaobo Guo; Jing Lin; Wei Wang; Jinghong Chen
LncRNA myocardial infarction associated transcript (MIAT) has been shown to be involved in osteoarthritis (OA), but its role in Kashin-Beck Disease (KBD) has rarely been reported. In this study, rats were administered with low selenium and/or T-2 toxin for 4 weeks to establish a KBD animal model. The serum selenium level, TNF-α and IL-1β contents, phosphorylated p65 (p-p65) and MIAT expression were
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Inhibition of osteoblast proliferation and migration by exogenous and endogenous formaldehyde Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-25 Xu Teng; Pei Wang; Tianshu Yang; Wei Huang; Hefeng Yu; Weishi Li; Zhongqiang Chen; Dongwei Fan
Exogenous and endogenous formaldehyde (FA) both play an important role in cell growth and migration; however, their potential role in osteoblasts remains largely unclear. Cell counting kit-8 (CCK-8) and wound-healing assays revealed that FA exposure at naturally occurring concentrations inhibited the proliferation and migration of mouse preosteoblast MC3T3-E1 cells. Moreover, RNA sequencing (RNA-seq)
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Influence of VDR and HFE polymorphisms on blood lead levels of occupationally exposed workers Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-25 Monica Shirley Mani; Amitha Puranik; Shama Prasada Kabekkodu; Manjunath B Joshi; Herman Sunil Dsouza
Lead is a ubiquitous heavy metal toxin of significant public health concern. Every individual varies in their response to lead’s toxic effects due to underlying genetic variations in lead metabolizing enzymes or proteins distributed in the population. Earlier studies, including our lab, have attributed the influence of ALAD (δ-Aminolevulinate dehydratase) polymorphism on blood lead retention and ALAD
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Alpha-lipoic acid ameliorates cytarabine-induced developmental anomalies in rat fetus Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-23 Ramanachary Namoju; Naga Kavitha Chilaka
Cytarabine (Ara-C) is a nucleoside analogue used in the treatment of cancers and viral infections. It has teratogenic potential and causes a variety of birth defects in fetuses. Alpha-lipoic acid (ALA) is a natural antioxidant offers protection against the developmental toxicity induced by drug- or toxicant-exposure or pathological conditions. This study was aimed at evaluating the protective effect
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Melatonin protects against tenofovir-induced nephrotoxicity in rats by targeting multiple cellular pathways Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-11-04 Hemalatha Ramamoorthy; Premila Abraham; Bina Isaac
Nephrotoxicity is a dose-limiting side effect of long-term use of tenofovir, a reverse transcriptase inhibitor that is used for the treatment of HIV infection and chronic hepatitis B infection. Identifying an agent that prevents tenofovir disoproxil fumarate (TDF)-induced renal injury can lead to its better tolerance, and a more effective treatment can be achieved. The present study is aimed at investigating
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Effects of TNF-α antagonist infliximab on fructose-induced metabolic syndrome in rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-29 Yasmine A Abdelhamid; Mohammed F Elyamany; Muhammad Y Al-Shorbagy; Osama A Badary
Public health issues have been raised regarding fructose toxicity and its serious metabolic disorders. Deleterious effects of high fructose intake on insulin sensitivity, body weight, lipid homeostasis have been identified. The new millennium has witnessed the emergence of a modern epidemic, the metabolic syndrome (MS), in approximately 25% of the world’s adult population. The current study aimed to
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Induction of apoptosis in indole-3-carbinol-treated lung cancer H1299 cells via ROS level elevation Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-29 Heui Min Lim; See-Hyoung Park; Myeong Jin Nam
This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate the induction of growth arrest and/or cell death in H1299 cells, a cell cycle arrest assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay, and reactive oxygen species (ROS) detection
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Alpha-lipoic acid alleviates colistin nephrotoxicity in rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-28 Mehmet Asi Oktan; Cihan Heybeli; Cemre Ural; Ayse Kocak; Gokcen Bilici; Zahide Cavdar; Seda Ozbal; Sevki Arslan; Osman Yilmaz; Caner Cavdar
Colistin methanesulfonate (CMS), a clinical form of colistin, is widely used as a last-line treatment for multidrug-resistant (MDR) gram-negative bacterial infections in critically ill patients presenting a considerably high mortality rate. However, nephrotoxicity is considered to be a critical adverse effect that limits CMS’s clinical use. Alpha-lipoic acid (ALA) is a strong antioxidant that is effective
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Immunopathological effects of experimental T-2 mycotoxicosis in Wistar rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-28 Shafiqur Rahman; Anil Kumar Sharma; Nittin Dev Singh; Shahid Prawez
It is well known that T-2 toxin has cytotoxic radiomimetic like effects on the immune system. Because of scant research data demonstrating the chronic effects of low doses of the T-2 toxin on humoral and cellular responses in rats, the present experiment was undertaken. The animals were divided into four groups, namely, group I (0.5 ppm), group II (0.75 ppm) and group III (1.0 ppm) and group IV (control)
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Inhibition of sphingomyelin synthase 2 relieves hypoxia-induced cardiomyocyte injury by reinforcing Nrf2/ARE activation via modulation of GSK-3β Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-28 Aiping Jin; Haijuan Cheng; Lina Xia; Sha Ye; Cuiling Yang
Sphingomyelin synthase 2 (SMS2) is a vital contributor to tissue injury and affects various pathological processes. However, whether SMS2 participates in the modulation of cardiac injury in myocardial infarction has not been determined. This study aimed to evaluate the potential role of SMS2 in the regulation of cardiomyocyte injury induced by hypoxia, an in vitro model for studying myocardial infarction
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Suppression of bromodomain-containing protein 4 protects trophoblast cells from oxidative stress injury by enhancing Nrf2 activation Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-23 Yiqing Wu; Yang Mi; Fan Zhang; Yimin Cheng; Xiaoling Wu
Oxidative stress is considered a key hallmark of preeclampsia, which causes the dysregulation of trophoblast cells, and it contributes to the pathogenesis of preeclampsia. Emerging evidence has suggested bromodomain-containing protein 4 (BRD4) as a key regulator of oxidative stress in multiple cell types. However, whether BRD4 participates in regulating oxidative stress in trophoblast cells remains
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Scorpion sting: N-terminal fragment of proB-type natriuretic peptide as an early predictor of pediatric cardiotoxicity Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-23 Mohamed AM Khalaf; Mohamed A Bahaa El-deen; Asmaa Mohammed Hishmat
Aim of the work: This prospective study was conducted to evaluate the clinical reliability of N-terminal fragment of proB-type natriuretic peptide (NT-proBNP) in identifying patients with cardiotoxicity in the early hours following scorpion sting. Subjects and methods: This study involved 483 children aging between 2–17 years who were admitted to Minia University Poisoning Control Center from 1st January
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Heat shock protein 70 expression protects against sepsis-associated cardiomyopathy by inhibiting autophagy Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-19 Xiaofeng Wang; Yan Zhu; Qiuxiang Zhou; Yueyue Yan; Jinlong Qu; Hongwei Ye
Objectives: Increasing evidence suggests that heat shock protein 70 (Hsp70) has a protective effect in sepsis-induced cardiomyopathy; however, the protective mechanism remains unclear. Methods: Previous studies have also implicated autophagy in sepsis-induced cardiomyopathy. The aim of the current study was to reveal the protective mechanisms of Hsp70 in sepsis-induced cardiomyopathy using a cecal
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Inorganic arsenic administration suppresses human neutrophil function in vitro Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-16 Md. Monirul Islam; Naoshi Takeyama
Arsenic, a major environmental toxicant and pollutant, is a global public health concern. Among its many adverse effects, arsenic is immunotoxic, but its effects on human neutrophil functions are not yet well-defined. In this study, we aimed to evaluate the in vitro effects of acute low-dose NaAsO2 exposure on human polymorphonuclear neutrophils (PMNs) for 12 h on the following innate defense mechanisms:
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Lipid emulsion attenuates extrinsic apoptosis induced by amlodipine toxicity in rat cardiomyoblasts Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-08 Seong-Ho Ok; Seung Hyun Ahn; Hyun-Jin Kim; Soo Hee Lee; Sung Il Bae; Kyeong-Eon Park; Yeran Hwang; Il-Woo Shin; Sangcheol Yoon; Ju-Tae Sohn
Amlodipine-induced toxicity has detrimental effects on cardiac cells. The aim of this study was to examine the effect of lipid emulsion on decreased H9c2 rat cardiomyoblast viability induced by amlodipine toxicity. The effects of amlodipine, lipid emulsion, LY 294002, and glibenclamide, either alone or in combination, on cell viability and count, apoptosis, and expression of cleaved caspase-3 and -8
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Glycyrrhizic acid ameliorates sodium nitrite-induced lung and salivary gland toxicity: Impact on oxidative stress, inflammation and fibrosis Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-08 Amira M Elsherbini; Nadia M Maysarah; Mohamed El-Sherbiny; Mohammed MH Al-Gayyar; Nehal M Elsherbiny
Despite wide application of sodium nitrite (SN) as food additive, it exhibits considerable side effects on various body organs at high dose or chronic exposure. The aim of this study was to test whether Glycyrrhizic acid (GA) could ameliorate SN-induced toxicity in lung and submandibular salivary gland (SMG). A sample size of 30 adult male albino rats was randomly allocated into 3 groups. Group 1 served
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Apelin-13 protects the lungs from ischemia-reperfusion injury by attenuating inflammatory and oxidative stress Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-07 F Xia; H Chen; Z Jin; Z Fu
Apelin has been reported to regulate mitochondrial function in myocardial ischemia-reperfusion injury and cerebral ischemia-reperfusion injury. However, the role of apelin-13 in lung ischemia-reperfusion injury (LIRI) remains unclear. This study established an experimental rat model to evaluate the underlying mechanisms of apelin-13 on LIRI. Twenty-four rats were randomly divided to sham operation
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Protective effects of quercetin and crocin in the kidneys and liver of obese Sprague-Dawley rats with Type 2 diabetes: Effects of quercetin and crocin on T2DM rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-06 Ling-Lin Lai; Hui-Qin Lu; Wen-Na Li; Hui-Ping Huang; He-Ying Zhou; En-nian Leng; Yue-yue Zhang
Quercetin and crocin are the main active constituents of Eucommia and Gardenia species, respectively. This study was conducted to explore the effects of quercetin and crocin on fat reduction and renal fibrosis and the relationship of these compounds with autophagy. First, a model of high-fat diet- and streptozotocin-induced type 2 diabetes was established and then subjected model animals to 8 weeks
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The cross-talk of NLRP3 inflammasome activation and necroptotic hepatocyte death in acetaminophen-induced mice acute liver injury Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-06 L Li; S Shan; K Kang; C Zhang; R Kou; F Song
Overdose acetaminophen (APAP) can result in severe liver injury, which is responsible for nearly half of drug-induced liver injury in western countries. Previous studies have found that there existed massive hepatocellular necrosis and severe inflammatory response in APAP-induced liver injury. However, the mechanistic linkage between necroptosis and NLRP3 inflammasome pathway in APAP-induced hepatotoxicity
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Pyrvinium pamoate induces in-vitro suppression of IL-6 and IL-8 produced by human endometriotic stromal cells Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-10-06 Amin Karamian; Shahrokh Paktinat; Sahar Esfandyari; Hamid Nazarian; Seyed Ali Ziai; Amir-Hassan Zarnani; Saghar Salehpour; Hossein Hosseinirad; Armin Karamian; Marefat Ghaffari Novin
Endometriosis, a chronic inflammatory disease, is identified by the presence of endometrial tissue outside the uterus. The prevalence of this disease among reproductive-age women is almost 10–15%. High levels of IL-6 and IL-8 have been found in the peritoneal fluid (PF) of women with endometriosis and are involved in its pathogenesis. Isolated stromal cells from 12 ectopic and eutopic endometrial biopsies
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The effect of imatinib administered in the prenatal period on testis development in rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-29 Z Topal Suzan; L Tumkaya; T Mercantepe; M Atak; HA Uydu
Background: The purpose of this study was to examine the effects of exposure to imatinib in the prenatal period on testis development in rats. Methods: Although all the study groups received intraperitoneal imatinib on prenatal days 1–8, no pregnancy occurred in the Imatinib-80 group. Immunohistochemical analysis, TUNEL, c-kit and PDGF staining revealed no difference between the groups in terms of
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Gas explosion-induced acute blast lung injury assessment and biomarker identification by a LC-MS-based serum metabolomics analysis. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-24 X Dong,S Yao,W Wu,J Cao,L Sun,H Li,H Ren,W Ren
The objective of this study was to evaluate the histopathological effect of gas explosion on rats, and to explore the metabolic alterations associated with gas explosion-induced acute blast lung injury (ABLI) in real roadway environment using metabolomics analyses. All rats were exposed to the gas explosion source at different distance points (160 m and 240 m) except the control group. Respiratory
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MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-22 H-J Wang,H Liu,Y-H Lin,S-J Zhang
Diabetic nephropathy (DN) is primary cause of end-stage renal disease. A previous study has shown that miR-32-5p (miR-32) is highly expressed in kidney tissue during chronic allograft dysfunction with interstitial fibrosis and tubular atrophy. However, the role of miR-32-5p (miR-32) in DN is still unclear. In this study, streptozotocin-induced DN rat models and high glucose (HG)-incubated human kidney
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Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-22 M A Ibrahim,Masa Haleem,S A AbdelWahab,A M Abdel-Aziz
Alzheimer disease (AD) is a chronic neurodegenerative disease with multi-pathways pathogenesis. Sildenafil is a selective phosphodiesterase-5 inhibitor with a potential benefit in the treatment of AD. This study investigated the possible mechanisms underlying the effect of sildenafil in AD with emphasis on vascular endothelial growth factor (VEGF), and vascular cell adhesion molecule-1 (VCAM-1). Twenty-four
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Morroniside suppresses hydrogen peroxide-stimulated autophagy and apoptosis in rat ovarian granulosa cells through the PI3K/AKT/mTOR pathway. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-21 D Deng,J Yan,Y Wu,K Wu,W Li
Previous evidences have indicated that granulosa cells play a critical role in follicular growth. Hydrogen peroxide (H2O2)-induced oxidative stress has been associated with ovarian granulosa cell apoptosis and ovarian function. Recently, a study highlighted the protective role of morroniside against H2O2-induced damage. In this study, we aimed to investigate the effects of morroniside on H2O2-stimulated
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Effects of baohuoside-I on epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-18 Q Wang,S Jiang,W Wang,H Jiang
To investigate the effect of baohuoside-I against nasopharyngeal carcinoma (NPC) and its underlying mechanism, baohuoside-I was employed to treat NPC cell lines CNE1 and CNE2 in vitro, followed by attachment and detachment assays to evalute the epithelial-mesenchymal transition (EMT) phenotype markers. Baohuoside-I was also administered to experimental mice to assess its effect on xenograft tumor growth
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Resveratrol prevents acute renal injury in a model of ruptured abdominal aortic aneurysm. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-17 D Hemsinli,L Tumkaya,S Ergene,S O Karakisi,T Mercantepe,S Çınar,A Yilmaz
Objective: To examine the biochemical and histopathological effects of ischemia/reperfusion (I/R) injury in a ruptured abdominal aortic aneurysm (RAAA) model in rats, and to investigate the potential protective role of resveratrol. Methods: Thirty-two male Sprague-Dawley rats were randomly assigned into four groups—control, I/R, sham (I/R + solvent/dimethyl sulfoxide), and I/R + resveratrol. The control
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In vivo assessment of bisphenol A induced histopathological alterations and inflammatory gene expression in lungs of male Wistar rats Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-16 A Rehman; T Akhtar; N Hameed; N Sheikh
Bisphenol A (BPA), an imperative environmental contaminant used in polycarbonate plastics. Due to limited information concerning the effect of BPA on lungs, this study design to assess whether BPA cause alterations in histopathology and trace metal content in lungs of rats. They were divided into five groups with five rats per group. Group I was named as control group. Group L6 and L12 were received
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Melatonin attenuates chemical-induced cardiotoxicity. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-16 S Zare,F S Heydari,A W Hayes,R J Reiter,M R Zirak,G Karimi
Environmental chemicals and drugs can induce cardiotoxicity, mainly by generating free radicals. Reactive oxygen species play a critical role in the pathogenesis of cardiac tissue injury. This highlights a need for prevention of cardiotoxicity by scavenging free radicals. Melatonin has been shown to act as a protector against various conditions in which free radicals cause molecular and tissue injury
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Toll-like receptor 4 prevents AOM/DSS-induced colitis-associated colorectal cancer in Bacteroides fragilis gnotobiotic mice. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-14 Yen-Peng Lee,Wen-Ching Huang,Tien-Jen Lin,Chien-Chao Chiu,Yu-Chih Wang,Yi-Hsun Chen,Shao-Wen Hung,Hsiao-Li Chuang,Ter-Hsin Chen
Bacteroides fragilis (BF) plays a critical role in developing and maintaining the mammalian immune system. We previously found that BF colonization could prevent inflammation and tumor formation in a germ-free (GF) colitis-associated colorectal cancer (CAC) mouse model. The role of Toll-like receptor 4 (TLR4) in CAC development has not been clearly elucidated in BF mono-colonized gnotobiotic mice.
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Possible mechanism of bamboo shoots (Bambusa balcooa) induced thyroid disruption - An in vitro study. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 D Sarkar,A K Chandra,S Chattopadyay,M Biswas,S Das,L H Singh,I Ray
Endemic goitre and associated iodine deficiency disorders (IDDs) are a major concern in public health even in the period of post salt iodization in many regions. Among others the consumption of cyanogenic plants found responsible for the persistence of such diseases. Bamboo shoots (BS) is one such cyanogenic plant food that caused disruption of certain thyroid hormone synthesizing regulatory element
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Ablation of small conductance calcium-activated potassium type-2 channel (SK2) delays occurrence of bupivacaine-induced cardiotoxicity in isolated mouse hearts. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 H Chen,F Xia,X Chen,Y Cai,Z Jin
Bupivacaine is frequently used for conducting regional anesthesia. When accidentally injected or excessively absorbed into circulation, bupivacaine can induce severe arrhythmia and potentially lead to cardiac arrest. The specific mechanisms underlying this cardiotoxicity, however, remain to be clarified. We transfected HEK-293 cells to express the small conductance calcium-activated potassium type-2
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Synthesis of PEGylated nanographene oxide as a nanocarrier for docetaxel drugs and anticancer activity on prostate cancer cell lines. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 Ayca Tas,Nese Keklikcioglu Cakmak
Graphene oxide (GO) has recently been considered one of the most promising carbon derivatives in nanotechnology. It has many excellent features such as tumor targeting ability, biocompatibility and low toxicity. Therefore, we conjugated docetaxel (DTX) to GO-PEG molecule and investigate its anticancer efficacy in prostate cancer cell line (DU-145). In order to obtain GO-PEG-DTX molecules, we conjugated
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An approach to evaluating the potential teratogenic and neurotoxic mechanism of BHA based on apoptosis induced by oxidative stress in zebrafish embryo (Danio rerio). Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 A Baran,S Yildirim,A Ghosigharehaghaji,İ Bolat,E Sulukan,S B Ceyhun
Butylated hydroxyanisole (BHA) has been widely used in the cosmetics, pharmaceutical, and food industries due to its antioxidant activity. Despite the antioxidant effects, reported adverse effects of BHA at the cellular level have made its use controversial. In this regard, this study was performed to elucidate the potential toxicity mechanism caused by BHA at the molecular level in zebrafish embryos
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Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 R Reis,H Sipahi,O Dinc,T Kavaz,M Charehsaz,A Dimoglo,A Aydın
Over the last decade, electrolyzed water (EW) produced by salt and tap water has gained importance due to its antimicrobial effects. Regarding to chlorine-based compounds, EW also used in post-harvest safety of food processing and sterilization of surfaces. The latest studies suggested that EW might act as wound healing agent due to anti-infective and cell proliferative properties. In this study, we
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Senescence marker protein 30 confers neuroprotection in oxygen-glucose deprivation/reoxygenation-injured neurons through modulation of Keap1/Nrf2 signaling: Role of SMP30 in OGD/R-induced neuronal injury. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 Wenxiong Liu,Haikang Zhao,Yuqiang Su,Kefeng Wang,Jing Li,Sha Xue,Xiaopeng Sun,Zhengguo Qiu
Senescence marker protein 30 (SMP30) is a senescence marker molecule and identified as a calcium regulatory protein. Currently, SMP30 has emerged as a cytoprotective protein in a wide range of cell types. However, the role of SMP30 in regulating neuronal survival during cerebral ischemia/reperfusion injury remains unclear. In the present study, we aimed to investigate the biological function and regulatory
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MicroRNA-155 is upregulated in the placentas of patients with preeclampsia and affects trophoblast apoptosis by targeting SHH/GLi1/BCL2. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 Zhenpeng Wang,Yanhong Shan,Yi Yang,Tianshu Wang,Zhiheng Guo
The pathogenesis of preeclampsia (PE) is complicated and multiple risk factors have been associated with its occurrence. Still, the underlying molecular mechanisms involved in PE remain elusive. Aberrant apoptosis and insufficient invasion of trophoblasts have been observed and are considered vital pathological features in PE. Herein, we found that miR-155 can specifically degrade the mRNA of the Hedgehog
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Gene expression in rat placenta after exposure to di(2-ethylhexyl) phthalate. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 Wan Xu,Hongyan Wu,Lixin Shang
The organic compound di(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in many products. Exposure to DEHP has been reported to lead to adverse pregnancy outcomes by suppressing placenta growth and development. The aim of this study was to determine the gene expression profiles of rat placenta exposed to (DEHP) and identify genes crucial for the DEHP response. Three groups of Wistar
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Hepatoprotective effect of Omega-3 PUFAs against acute paracetamol-induced hepatic injury confirmed by FTIR. Hum. Exp. Toxicol. (IF 2.067) Pub Date : 2020-09-10 Zeinab A El-Gendy,Seham A El-Batran,Sah Youssef,A Ramadan,Walid El Hotaby,Rofanda M Bakeer,Rania F Ahmed
Acute paracetamol over dose-induced hepatotoxicity is considered an important medical hazard especially among women. Omega-3 long-chain polyunsaturated fatty acids (Omega-3 PUFAs) daily doses are nowadays recommended for their antioxidant and anti-inflammatory potentials. Fourier transform infrared (FTIR) spectroscopy is considered a reliable method in analyzing cellular alterations