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Linking Gut Microbiome and Lipid Metabolism: Moving beyond Associations Metabolites (IF 4.097) Pub Date : 2021-01-15 Santosh Lamichhane; Partho Sen; Marina Amaral Alves; Henrique C. Ribeiro; Peppi Raunioniemi; Tuulia Hyötyläinen; Matej Orešič
Various studies aiming to elucidate the role of the gut microbiome-metabolome co-axis in health and disease have primarily focused on water-soluble polar metabolites, whilst non-polar microbial lipids have received less attention. The concept of microbiota-dependent lipid biotransformation is over a century old. However, only recently, several studies have shown how microbial lipids alter intestinal
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Exploring the Characteristic Aroma of Beef from Japanese Black Cattle (Japanese Wagyu) via Sensory Evaluation and Gas Chromatography-Olfactometry Metabolites (IF 4.097) Pub Date : 2021-01-15 Shuji Ueda; Minoru Yamanoue; Yasuhito Sirai; Eiji Iwamoto
Beef from Japanese Black cattle (Japanese Wagyu) is renowned for its flavor characteristics. To clarify the key metabolites contributing to this rich and sweet aroma of beef, an omics analysis combined with GC-olfactometry (GC-O) and metabolomics analysis with gas chromatography–mass spectrometry (GC-MS) were applied. GC-O analysis identified 39 odor-active odorants from the volatile fraction of boiled
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Simultaneous Measurement of Amino Acid Enantiomers in Aged Mouse Brain Samples by LC/MS/MS Combined with Derivatization Using Nα-(5-Fluoro-2,4-dinitrophenyl)-l-leucinamide (l-FDLA) Metabolites (IF 4.097) Pub Date : 2021-01-15 Taiji Yamamoto; Keisuke Yaku; Takashi Nakagawa
D-amino acids have distinct roles from their l-enantiomer. In particular, some D-amino acids function as agonists or antagonists of neuronal receptors and are involved in higher brain functions. Thus, it is important to precisely measure the levels of these amino acid enantiomers in cells and tissues. Various quantification methods have been developed for measurements of chiral amino acids. However
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L-Carnitine and Acylcarnitines: Mitochondrial Biomarkers for Precision Medicine Metabolites (IF 4.097) Pub Date : 2021-01-14 Marc R. McCann; Mery Vet George De la Rosa; Gus R. Rosania; Kathleen A. Stringer
Biomarker discovery and implementation are at the forefront of the precision medicine movement. Modern advances in the field of metabolomics afford the opportunity to readily identify new metabolite biomarkers across a wide array of disciplines. Many of the metabolites are derived from or directly reflective of mitochondrial metabolism. L-carnitine and acylcarnitines are established mitochondrial biomarkers
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Comprehensive Comparative Analysis of Local False Discovery Rate Control Methods Metabolites (IF 4.097) Pub Date : 2021-01-14 Shin June Kim; Youngjae Oh; Jaesik Jeong
Due to the advance in technology, the type of data is getting more complicated and large-scale. To analyze such complex data, more advanced technique is required. In case of omics data from two different groups, it is interesting to find significant biomarkers between two groups while controlling error rate such as false discovery rate (FDR). Over the last few decades, a lot of methods that control
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Beyond Proteostasis: Lipid Metabolism as a New Player in ER Homeostasis Metabolites (IF 4.097) Pub Date : 2021-01-14 Jiaming Xu; Stefan Taubert
Biological membranes are not only essential barriers that separate cellular and subcellular structures, but also perform other critical functions such as the initiation and propagation of intra- and intercellular signals. Each membrane-delineated organelle has a tightly regulated and custom-made membrane lipid composition that is critical for its normal function. The endoplasmic reticulum (ER) consists
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Two Metabolomics Phenotypes of Human Hepatocellular Carcinoma in Non-Alcoholic Fatty Liver Disease According to Fibrosis Severity Metabolites (IF 4.097) Pub Date : 2021-01-14 Benjamin Buchard; Camille Teilhet; Natali Abeywickrama Samarakoon; Sylvie Massoulier; Juliette Joubert-Zakeyh; Corinne Blouin; Christelle Reynes; Robert Sabatier; Anne-Sophie Biesse-Martin; Marie-Paule Vasson; Armando Abergel; Aicha Demidem
Non-Alcoholic Fatty Liver Disease (NAFLD) is considered as the forthcoming predominant cause for hepatocellular carcinoma (HCC). NAFLD-HCC may rise in non-cirrhotic livers in 40 to 50% of patients. The aim of this study was to identify different metabolic pathways of HCC according to fibrosis level (F0F1 vs. F3F4). A non-targeted metabolomics strategy was applied. We analyzed 52 pairs of human HCC
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Diverse Taxonomies for Diverse Chemistries: Enhanced Representation of Natural Product Metabolism in UniProtKB Metabolites (IF 4.097) Pub Date : 2021-01-12 Marc Feuermann; Emmanuel Boutet; Anne Morgat; Kristian B. Axelsen; Parit Bansal; Jerven Bolleman; Edouard de Castro; Elisabeth Coudert; Elisabeth Gasteiger; Sébastien Géhant; Damien Lieberherr; Thierry Lombardot; Teresa B. Neto; Ivo Pedruzzi; Sylvain Poux; Monica Pozzato; Nicole Redaschi; Alan Bridge; on behalf of the UniProt Consortium
The UniProt Knowledgebase UniProtKB is a comprehensive, high-quality, and freely accessible resource of protein sequences and functional annotation that covers genomes and proteomes from tens of thousands of taxa, including a broad range of plants and microorganisms producing natural products of medical, nutritional, and agronomical interest. Here we describe work that enhances the utility of UniProtKB
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Trimethylornithine Membrane Lipids: Discovered in Planctomycetes and Identified in Diverse Environments Metabolites (IF 4.097) Pub Date : 2021-01-12 Eli K. Moore
Intact polar membrane lipids (IPLs) are the building blocks of all cell membranes. There is a wide range of phosphorus-free IPL structures, including amino acid containing IPLs, that can be taxonomically specific. Trimethylornithine membrane lipids (TMOs) were discovered in northern wetland Planctomycete species that were isolated and described in the last decade. The trimethylated terminal nitrogen
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In Vivo Metabolism of [1,6-13C2]Glucose Reveals Distinct Neuroenergetic Functionality between Mouse Hippocampus and Hypothalamus Metabolites (IF 4.097) Pub Date : 2021-01-12 Antoine Cherix; Rajesh Sonti; Bernard Lanz; Hongxia Lei
Glucose is a major energy fuel for the brain, however, less is known about specificities of its metabolism in distinct cerebral areas. Here we examined the regional differences in glucose utilization between the hypothalamus and hippocampus using in vivo indirect 13C magnetic resonance spectroscopy (1H-[13C]-MRS) upon infusion of [1,6-13C2]glucose. Using a metabolic flux analysis with a 1-compartment
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Free Saturated Oxo Fatty Acids (SOFAs) and Ricinoleic Acid in Milk Determined by a Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS) Method Metabolites (IF 4.097) Pub Date : 2021-01-11 Maroula G. Kokotou; Charikleia S. Batsika; Christiana Mantzourani; George Kokotos
Oxidized saturated fatty acids, containing a hydroxyl or an oxo functionality, have attracted little attention so far. Recent studies have shown that saturated hydroxy fatty acids, which exhibit cancer cell growth inhibition and may suppress β-cell apoptosis, are present in milk. Herein, we present the application of a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for the
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Analytical Methods for Quantification and Identification of Intact Glucosinolates in Arabidopsis Roots Using LC-QqQ(LIT)-MS/MS Metabolites (IF 4.097) Pub Date : 2021-01-11 Kourosh Hooshmand; Inge S. Fomsgaard
Glucosinolates are biologically active secondary metabolites in Brassicaceae plants that play a critical role in positive and negative interactions between plants and root-associated microbial communities. The aim of this study was to develop a reversed-phase liquid chromatography method to quantify and identify intact glucosinolates in the root of Arabidopsis thaliana (Arabidopsis) grown in non-sterile
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Comprehensive Meta-Analysis of COVID-19 Global Metabolomics Datasets Metabolites (IF 4.097) Pub Date : 2021-01-09 Zhiqiang Pang; Guangyan Zhou; Jasmine Chong; Jianguo Xia
The novel coronavirus SARS-CoV-2 has spread across the world since 2019, causing a global pandemic. The pathogenesis of the viral infection and the associated clinical presentations depend primarily on host factors such as age and immunity, rather than the viral load or its genetic variations. A growing number of omics studies have been conducted to characterize the host immune and metabolic responses
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Tissue-Specific 1H-NMR Metabolomic Profiling in Mice with Adenine-Induced Chronic Kidney Disease Metabolites (IF 4.097) Pub Date : 2021-01-10 Ram B. Khattri; Trace Thome; Terence E. Ryan
Chronic kidney disease (CKD) results in the impaired filtration of metabolites, which may be toxic or harmful to organs/tissues. The objective of this study was to perform unbiased 1H nuclear magnetic resonance (NMR)-based metabolomics profiling of tissues from mice with CKD. Five-month-old male C57BL6J mice were placed on either a casein control diet or adenine-supplemented diet to induce CKD for
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Comparison of Regular, Pure Shift, and Fast 2D NMR Experiments for Determination of the Geographical Origin of Walnuts Metabolites (IF 4.097) Pub Date : 2021-01-08 Stephanie Watermann; Caroline Schmitt; Tobias Schneider; Thomas Hackl
1H NMR spectroscopy, in combination with chemometric methods, was used to analyze the methanol/acetonitrile (1:1) extract of walnut (Juglans Regia L.) regarding the geographical origin of 128 authentic samples from different countries (France, Germany, China) and harvest years (2016–2019). Due to the large number of different metabolites within the acetonitrile/methanol extract, the one-dimensional
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HPLC-HRMS Global Metabolomics Approach for the Diagnosis of “Olive Quick Decline Syndrome” Markers in Olive Trees Leaves Metabolites (IF 4.097) Pub Date : 2021-01-08 Alberto Asteggiano; Pietro Franceschi; Michael Zorzi; Riccardo Aigotti; Federica Dal Bello; Francesca Baldassarre; Francesco Lops; Antonia Carlucci; Claudio Medana; Giuseppe Ciccarella
Olive quick decline syndrome (OQDS) is a multifactorial disease affecting olive plants. The onset of this economically devastating disease has been associated with a Gram-negative plant pathogen called Xylella fastidiosa (Xf). Liquid chromatography separation coupled to high-resolution mass spectrometry detection is one the most widely applied technologies in metabolomics, as it provides a blend of
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Bioactive Ether Lipids: Primordial Modulators of Cellular Signaling Metabolites (IF 4.097) Pub Date : 2021-01-08 Nikhil Rangholia; Tina M. Leisner; Stephen P. Holly
The primacy of lipids as essential components of cellular membranes is conserved across taxonomic domains. In addition to this crucial role as a semi-permeable barrier, lipids are also increasingly recognized as important signaling molecules with diverse functional mechanisms ranging from cell surface receptor binding to the intracellular regulation of enzymatic cascades. In this review, we focus on
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Untargeted Metabolomics Analysis Using FTIR and UHPLC-Q-Orbitrap HRMS of Two Curculigo Species and Evaluation of their Antioxidant and α-Glucosidase Inhibitory Activities Metabolites (IF 4.097) Pub Date : 2021-01-08 Abdul Halim Umar; Diah Ratnadewi; Mohamad Rafi; Yohana Caecilia Sulistyaningsih
Curculigo orchioides and C. latifolia have been used as traditional medicines such as antidiabetic and anticancer. This study measured the total phenolics and flavonoid contents as well as analyzed the functional groups and chemical compounds using Fourier-transform infrared (FTIR) spectra and UHPLC-Q-Orbitrap-HRMS profiling for the discrimination of plant parts, geographical origin, and compounds
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Molecular Differences Based on Erythrocyte Fatty Acid Profile to Personalize Dietary Strategies between Adults and Children with Obesity Metabolites (IF 4.097) Pub Date : 2021-01-08 Iker Jauregibeitia; Kevin Portune; Sonia Gaztambide; Itxaso Rica; Itziar Tueros; Olaia Velasco; Gema Grau; Alicia Martín; Luis Castaño; Anna Vita Larocca; Federica Di Nolfo; Carla Ferreri; Sara Arranz
As the obesity epidemic continues to grow inexorably worldwide, the need to develop effective strategies to prevent and control obesity seems crucial. The use of molecular tools can be useful to characterize different obesity phenotypes to provide more precise nutritional recommendations. This study aimed to determine the fatty acid (FA) profile of red blood cell (RBC) membranes, together with the
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Special Issue “Metabolic Engineering and Synthetic Biology Volume 2” Metabolites (IF 4.097) Pub Date : 2021-01-06 An N. T. Phan; Lars M. Blank
Note: In lieu of an abstract, this is an excerpt from the first page. In times of ever-increasing demand for chemicals and the subsequent increase in CO2 in the atmosphere, we have to intensify our efforts to establish a circular (bio) economy [...]
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Theoretical Analysis of the Built-in Metabolic Pathway Effect on the Metabolism of Erythrocyte-Bioreactors That Neutralize Ammonium Metabolites (IF 4.097) Pub Date : 2021-01-06 Evgeniy Protasov; Larisa Koleva; Elizaveta Bovt; Fazoil I. Ataullakhanov; Elena Sinauridze
The limitations of the efficiency of ammonium-neutralizing erythrocyte-bioreactors based on glutamate dehydrogenase and alanine aminotransferase reactions were analyzed using a mathematical model. At low pyruvate concentrations in the external medium (below about 0.3 mM), the main limiting factor is the rate of pyruvate influx into the erythrocyte from the outside, and at higher concentrations, it
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Involvements of Hyperhomocysteinemia in Neurological Disorders Metabolites (IF 4.097) Pub Date : 2021-01-06 Marika Cordaro; Rosalba Siracusa; Roberta Fusco; Salvatore Cuzzocrea; Rosanna Di Paola; Daniela Impellizzeri
Homocysteine (HCY), a physiological amino acid formed when proteins break down, leads to a pathological condition called hyperhomocysteinemia (HHCY), when it is over a definite limit. It is well known that an increase in HCY levels in blood, can contribute to arterial damage and several cardiovascular disease, but the knowledge about the relationship between HCY and brain disorders is very poor. Recent
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Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies Metabolites (IF 4.097) Pub Date : 2021-01-06 Annakatrin Häni; Gaëlle Diserens; Anna Oevermann; Peter Vermathen; Christina Precht
The metabolic profiling of tissue biopsies using high-resolution–magic angle spinning (HR-MAS) 1H nuclear magnetic resonance (NMR) spectroscopy may be influenced by experimental factors such as the sampling method. Therefore, we compared the effects of two different sampling methods on the metabolome of brain tissue obtained from the brainstem and thalamus of healthy goats by 1H HR-MAS NMR spectroscopy—in
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Application of Carbon-13 Isotopomer Analysis to Assess Perinatal Myocardial Glucose Metabolism in Sheep Metabolites (IF 4.097) Pub Date : 2021-01-05 Mukundan Ragavan; Mengchen Li; Anthony G. Giacalone; Charles E. Wood; Maureen Keller-Wood; Matthew E. Merritt
Ovine models of pregnancy have been used extensively to study maternal–fetal interactions and have provided considerable insight into nutrient transfer to the fetus. Ovine models have also been utilized to study congenital heart diseases. In this work, we demonstrate a comprehensive assessment of heart function and metabolism using a perinatal model of heart function with the addition of a [U-13C]glucose
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Amino Acid and Acylcarnitine Levels in Chronic Patients with Schizophrenia: A Preliminary Study Metabolites (IF 4.097) Pub Date : 2021-01-05 Irina A. Mednova; Alexander A. Chernonosov; Marat F. Kasakin; Elena G. Kornetova; Arkadiy V. Semke; Nikolay A. Bokhan; Vladimir V. Koval; Svetlana A. Ivanova
Amino acids and acylcarnitines play an important role as substrates and intermediate products in most of pathways involved in schizophrenia development such as mitochondrial dysfunction, inflammation, lipid oxidation, DNA damage, oxidative stress, and apoptosis. It seems relevant to use an integrated approach with ‘omics’ technology to study their contribution. The aim of our study was to investigate
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Gut Microbiota Dysbiosis Is Associated with Elevated Bile Acids in Parkinson’s Disease Metabolites (IF 4.097) Pub Date : 2021-01-04 Peipei Li; Bryan A. Killinger; Elizabeth Ensink; Ian Beddows; Ali Yilmaz; Noah Lubben; Jared Lamp; Meghan Schilthuis; Irving E. Vega; Randy Woltjer; J. Andrew Pospisilik; Patrik Brundin; Lena Brundin; Stewart F. Graham; Viviane Labrie
The gut microbiome can impact brain health and is altered in Parkinson’s disease (PD). The vermiform appendix is a lymphoid tissue in the cecum implicated in the storage and regulation of the gut microbiota. We sought to determine whether the appendix microbiome is altered in PD and to analyze the biological consequences of the microbial alterations. We investigated the changes in the functional microbiota
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Metabolic Detoxification of 2-Oxobutyrate by Remodeling Escherichia coli Acetate Bypass Metabolites (IF 4.097) Pub Date : 2021-01-04 Yu Fang; Shuyan Zhang; Jianli Wang; Lianghong Yin; Hailing Zhang; Zhen Wang; Jie Song; Xiaoqing Hu; Xiaoyuan Wang
2-Oxobutyrate (2-OBA), as a toxic metabolic intermediate, generally arrests the cell growth of most microorganisms and blocks the biosynthesis of target metabolites. In this study, we demonstrated that using the acetate bypass to replace the pyruvate dehydrogenase complex (PDHc) in Escherichia coli could recharge the intracellular acetyl-CoA pool to alleviate the metabolic toxicity of 2-OBA. Furthermore
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Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development Metabolites (IF 4.097) Pub Date : 2021-01-04 Rosa Perestrelo; Marijana Petkovic; Catarina Luís Silva
Altered lipid metabolism has been associated with the progression of various cancers, and aberrant expression of enzymes involved in the lipid metabolism has been detected in different stages of cancer. Breast cancer (BC) is one of the cancer types known to be associated with alterations in the lipid metabolism and overexpression of enzymes involved in this metabolism. It has been demonstrated that
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Quantitative Analysis of Biogenic Amines in Different Cheese Varieties Obtained from the Korean Domestic and Retail Markets Metabolites (IF 4.097) Pub Date : 2021-01-04 Sujatha Kandasamy; Jayeon Yoo; Jeonghee Yun; Han Byul Kang; Kuk-Hwan Seol; Jun-Sang Ham
To evaluate the safety and risk assessment of cheese consumption in the Republic of Korea, sixty cheese samples purchased from the farmstead and retails markets (imported) were analyzed for their biogenic amine (BA) contents. The BA profiles and quantities of eight amines (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine) were determined using high-performance
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The Harmonious Interplay of Amino Acid and Monocarboxylate Transporters Induces the Robustness of Cancer Cells Metabolites (IF 4.097) Pub Date : 2021-01-02 Go J. Yoshida
There is a growing body of evidence that metabolic reprogramming contributes to the acquisition and maintenance of robustness associated with malignancy. The fine regulation of expression levels of amino acid and monocarboxylate transporters enables cancer cells to exhibit the metabolic reprogramming that is responsible for therapeutic resistance. Amino acid transporters characterized by xCT (SLC7A11)
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Metabolic Reprogramming of Cancer Cells during Tumor Progression and Metastasis Metabolites (IF 4.097) Pub Date : 2021-01-02 Kenji Ohshima; Eiichi Morii
Cancer cells face various metabolic challenges during tumor progression, including growth in the nutrient-altered and oxygen-deficient microenvironment of the primary site, intravasation into vessels where anchorage-independent growth is required, and colonization of distant organs where the environment is distinct from that of the primary site. Thus, cancer cells must reprogram their metabolic state
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Total vs. Bioavailable: Determining a Better 25(OH)D Index in Association with Bone Density and Muscle Mass in Postmenopausal Women Metabolites (IF 4.097) Pub Date : 2020-12-31 Nurdiana Z. Abidin; Soma R. Mitra
The concurrent presence of low bone density (osteopenia/osteoporosis) and low muscle mass (sarcopenia) in older adults has led to the recognition of “osteosarcopenia” (OS) as a singular entity. Vitamin D may play important role in the manifestation of OS, in terms of intake, absorption, and bioavailability. Evidence suggests that bioavailable 25(OH)D may be a better indicator of Vitamin D compared
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An Ascophyllum nodosum-Derived Biostimulant Protects Model and Crop Plants from Oxidative Stress Metabolites (IF 4.097) Pub Date : 2020-12-31 Nikola S. Staykov; Mihail Angelov; Veselin Petrov; Pavel Minkov; Aakansha Kanojia; Kieran J. Guinan; Saleh Alseekh; Alisdair R. Fernie; Neerakkal Sujeeth; Tsanko S. Gechev
Abiotic stresses, which at the molecular level leads to oxidative damage, are major determinants of crop yield loss worldwide. Therefore, considerable efforts are directed towards developing strategies for their limitation and mitigation. Here the superoxide-inducing agent paraquat (PQ) was used to induce oxidative stress in the model species Arabidopsis thaliana and the crops tomato and pepper. Pre-treatment
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Lysophospholipids as Predictive Markers of ST-Elevation Myocardial Infarction (STEMI) and Non-ST-Elevation Myocardial Infarction (NSTEMI) Metabolites (IF 4.097) Pub Date : 2020-12-31 Elin Chorell; Tommy Olsson; Jan-Håkan Jansson; Patrik Wennberg
The present study explored patterns of circulating metabolites and proteins that can predict future risk for ST-elevation myocardial infarction (STEMI) and non-ST-elevation myocardial infarction (NSTEMI). We conducted a prospective nested case-control study in northern Sweden in individuals who developed STEMI (N = 50) and NSTEMI (N = 50) within 5 years and individually matched controls (N = 100).
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Reproducibility of Targeted Lipidome Analyses (Lipidyzer) in Plasma and Erythrocytes over a 6-Week Period Metabolites (IF 4.097) Pub Date : 2020-12-31 Marieke Loef; Johannes H. von Hegedus; Mohan Ghorasaini; Féline P.B. Kroon; Martin Giera; Andreea Ioan-Facsinay; Margreet Kloppenburg
It is essential to measure lipid biomarkers with a high reproducibility to prevent biased results. We compared the lipid composition and inter-day reproducibility of lipid measurements in plasma and erythrocytes. Samples from 42 individuals (77% women, mean age 65 years, mean body mass index (BMI) 27 kg/m2), obtained non-fasted at baseline and after 6 weeks, were used for quantification of up to 1000
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Decrease in Myelin-Associated Lipids Precedes Neuronal Loss and Glial Activation in the CNS of the Sandhoff Mouse as Determined by Metabolomics Metabolites (IF 4.097) Pub Date : 2020-12-30 Emmanuelle Lecommandeur; Maria Begoña Cachón-González; Susannah Boddie; Ben D. McNally; Andrew W. Nicholls; Timothy M. Cox; Julian L. Griffin
Sandhoff disease (SD) is a lysosomal disease caused by mutations in the gene coding for the β subunit of β-hexosaminidase, leading to deficiency in the enzymes β-hexosaminidase (HEX) A and B. SD is characterised by an accumulation of gangliosides and related glycolipids, mainly in the central nervous system, and progressive neurodegeneration. The underlying cellular mechanisms leading to neurodegeneration
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Development of Tandem Mass Tag Labeling Method for Lipid Molecules Containing Carboxy and Phosphate Groups, and Their Stability in Human Serum Metabolites (IF 4.097) Pub Date : 2020-12-30 Suzumi M. Tokuoka; Yoshihiro Kita; Masaya Sato; Takao Shimizu; Yutaka Yatomi; Yoshiya Oda
In clinical lipidomics, it is a challenge to measure a large number of samples and to reproduce the quantitative results. We expanded the range of application of the tandem mass tag (TMT) method, which is widely used in proteomics, to lipidomic fields. There are various types of lipid molecule, for example, eicosanoids have a carboxyl group and phosphatidic acid has a phosphate group. We modified these
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Identifying Personalized Metabolic Signatures in Breast Cancer Metabolites (IF 4.097) Pub Date : 2020-12-30 Priyanka Baloni; Wikum Dinalankara; John C. Earls; Theo A. Knijnenburg; Donald Geman; Luigi Marchionni; Nathan D. Price
Cancer cells are adept at reprogramming energy metabolism, and the precise manifestation of this metabolic reprogramming exhibits heterogeneity across individuals (and from cell to cell). In this study, we analyzed the metabolic differences between interpersonal heterogeneous cancer phenotypes. We used divergence analysis on gene expression data of 1156 breast normal and tumor samples from The Cancer
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Characterization of Volatile Organic Compounds in Mango Ginger (Curcuma amada Roxb.) from Myanmar Metabolites (IF 4.097) Pub Date : 2020-12-30 Yanhang Chen; Musavvara Kh. Shukurova; Yonathan Asikin; Miyako Kusano; Kazuo N. Watanabe
Curcuma amada Roxb. (Zingiberaceae), commonly known as mango ginger because its rhizome and foliar parts have a similar aroma to mango. The rhizome has been widely used in food industries and alternative medicines to treat a variety of internal diseases such as cough, bronchitis, indigestion, colic, loss of appetite, hiccups, and constipation. The composition of the volatile constituents in a fresh
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Sodium-Glucose Cotransporter-2 Inhibitors for Treatment of Nonalcoholic Fatty Liver Disease: A Meta-Analysis of Randomized Controlled Trials Metabolites (IF 4.097) Pub Date : 2020-12-30 Alessandro Mantovani; Graziana Petracca; Alessandro Csermely; Giorgia Beatrice; Giovanni Targher
Recent randomized controlled trials (RCTs) tested the efficacy of sodium-glucose cotransporter-2 (SGLT-2) inhibitors to specifically treat nonalcoholic fatty liver disease (NAFLD). We systematically searched three electronic databases (up to 31 October 2020) for identifying placebo-controlled or head-to-head RCTs that used SGLT-2 inhibitors for treatment of NAFLD. No published RCTs with paired liver
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SeMPI 2.0—A Web Server for PKS and NRPS Predictions Combined with Metabolite Screening in Natural Product Databases Metabolites (IF 4.097) Pub Date : 2020-12-29 Paul F. Zierep; Adriana T. Ceci; Ilia Dobrusin; Sinclair C. Rockwell-Kollmann; Stefan Günther
Microorganisms produce secondary metabolites with a remarkable range of bioactive properties. The constantly increasing amount of published genomic data provides the opportunity for efficient identification of biosynthetic gene clusters by genome mining. On the other hand, for many natural products with resolved structures, the encoding biosynthetic gene clusters have not been identified yet. Of those
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Holistic Metabolomic Laboratory-Developed Test (LDT): Development and Use for the Diagnosis of Early-Stage Parkinson’s Disease Metabolites (IF 4.097) Pub Date : 2020-12-29 Petr G. Lokhov; Dmitry L. Maslov; Steven Lichtenberg; Oxana P. Trifonova; Elena E. Balashova
A laboratory-developed test (LDT) is a type of in vitro diagnostic test that is developed and used within a single laboratory. The holistic metabolomic LDT integrating the currently available data on human metabolic pathways, changes in the concentrations of low-molecular-weight compounds in the human blood during diseases and other conditions, and their prevalent location in the body was developed
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Metabolomic Analysis of Response to Nitrogen-Limiting Conditions in Yarrowia spp. Metabolites (IF 4.097) Pub Date : 2020-12-29 Sivamoke Dissook; Sastia Prama Putri; Eiichiro Fukusaki
Yarrowia is a yeast genus that has been used as a model oleaginous taxon for a wide array of studies. However, information regarding metabolite changes within Yarrowia spp. under different environmental conditions is still limited. Among various factors affecting Yarrowia metabolism, nitrogen-limiting conditions have a profound effect on the metabolic state of yeast. In this study, a time-course LC-MS/MS-based
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Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species Metabolites (IF 4.097) Pub Date : 2020-12-29 Seham S. El-Hawary; Rabab Mohammed; Ahmed F. Tawfike; Nadia M. Lithy; Sameh Fekry AbouZid; Mohamed N. Amin; Usama Ramadan Abdelmohsen; Elham Amin
Euphorbia is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen Euphorbia species revealed highest activity of E. officinarum L. against CACO2 cell line (IC50 7.2 µM) and of E. lactea Haw. against HepG2 and MCF-7 cell lines (IC50 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using
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Urinary Volatile Organic Compound Analysis for the Diagnosis of Cancer: A Systematic Literature Review and Quality Assessment Metabolites (IF 4.097) Pub Date : 2020-12-29 Qing Wen; Piers Boshier; Antonis Myridakis; Ilaria Belluomo; George B. Hanna
The analysis of urinary volatile organic compounds (VOCs) is a promising field of research with the potential to discover new biomarkers for cancer early detection. This systematic review aims to summarise the published literature concerning cancer-associated urinary VOCs. A systematic online literature search was conducted to identify studies reporting urinary VOC biomarkers of cancers in accordance
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Discovery of Urinary Biomarkers of Seaweed Intake Using Untargeted LC–MS Metabolomics in a Three-Way Cross-Over Human Study Metabolites (IF 4.097) Pub Date : 2020-12-28 Muyao Xi; Lars Ove Dragsted; Mikkel Tullin; Madeleine Ernst; Nazikussabah Zaharudin; Giorgia La Barbera
Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In this three-armed, randomized, cross-over study, an untargeted metabolomics approach was applied for discovering
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Metabolic Dynamics of In Vitro CD8+ T Cell Activation Metabolites (IF 4.097) Pub Date : 2020-12-28 Joy Edwards-Hicks; Michael Mitterer; Erika L. Pearce; Joerg M. Buescher
CD8+ T cells detect and kill infected or cancerous cells. When activated from their naïve state, T cells undergo a complex transition, including major metabolic reprogramming. Detailed resolution of metabolic dynamics is needed to advance the field of immunometabolism. Here, we outline methodologies that when utilized in parallel achieve broad coverage of the metabolome. Specifically, we used a combination
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Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma Metabolites (IF 4.097) Pub Date : 2020-12-27 Corina Daniela Ene; Mircea Nicolae Penescu; Simona Roxana Georgescu; Mircea Tampa; Ilinca Nicolae
Posttranslational modifications are dynamic enzymatic-mediated processes, regulated in time and space, associated with cancer development. We aimed to evaluate the significance of posttranslational modifications in the pathogenesis of clear cell renal cell carcinoma. The authors developed a prospective, observational study during a period of three years and included 55 patients with localized renal
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Using Out-of-Batch Reference Populations to Improve Untargeted Metabolomics for Screening Inborn Errors of Metabolism Metabolites (IF 4.097) Pub Date : 2020-12-25 Michiel Bongaerts; Ramon Bonte; Serwet Demirdas; Edwin H. Jacobs; Esmee Oussoren; Ans T. van der Ploeg; Margreet A. E. M. Wagenmakers; Robert M. W. Hofstra; Henk J. Blom; Marcel J. T. Reinders; George J. G. Ruijter
Untargeted metabolomics is an emerging technology in the laboratory diagnosis of inborn errors of metabolism (IEM). Analysis of a large number of reference samples is crucial for correcting variations in metabolite concentrations that result from factors, such as diet, age, and gender in order to judge whether metabolite levels are abnormal. However, a large number of reference samples requires the
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Cholesterol Metabolites 25-Hydroxycholesterol and 25-Hydroxycholesterol 3-Sulfate Are Potent Paired Regulators: From Discovery to Clinical Usage Metabolites (IF 4.097) Pub Date : 2020-12-25 Yaping Wang; Xiaobo Li; Shunlin Ren
Oxysterols have long been believed to be ligands of nuclear receptors such as liver × receptor (LXR), and they play an important role in lipid homeostasis and in the immune system, where they are involved in both transcriptional and posttranscriptional mechanisms. However, they are increasingly associated with a wide variety of other, sometimes surprising, cell functions. Oxysterols have also been
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Continuous Glucose and Heart Rate Monitoring in Young People with Type 1 Diabetes: An Exploratory Study about Perspectives in Nocturnal Hypoglycemia Detection Metabolites (IF 4.097) Pub Date : 2020-12-24 Valeria Calcaterra; Pietro Bosoni; Lucia Sacchi; Gian Vincenzo Zuccotti; Savina Mannarino; Riccardo Bellazzi; Cristiana Larizza
A combination of information from blood glucose (BG) and heart rate (HR) measurements has been proposed to investigate the HR changes related to nocturnal hypoglycemia (NH) episodes in pediatric subjects with type 1 diabetes (T1D), examining whether they could improve hypoglycemia prediction. We enrolled seventeen children and adolescents with T1D, monitored on average for 194 days. BG was detected
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The Liability Threshold Model for Predicting the Risk of Cardiovascular Disease in Patients with Type 2 Diabetes: A Multi-Cohort Study of Korean Adults Metabolites (IF 4.097) Pub Date : 2020-12-24 Eun Pyo Hong; Seong Gu Heo; Ji Wan Park
Personalized risk prediction for diabetic cardiovascular disease (DCVD) is at the core of precision medicine in type 2 diabetes (T2D). We first identified three marker sets consisting of 15, 47, and 231 tagging single nucleotide polymorphisms (tSNPs) associated with DCVD using a linear mixed model in 2378 T2D patients obtained from four population-based Korean cohorts. Using the genetic variants with
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Evaluation of Metabolic Profiles of Patients with Anorexia Nervosa at Inpatient Admission, Short- and Long-Term Weight Regain—Descriptive and Pattern Analysis Metabolites (IF 4.097) Pub Date : 2020-12-24 Manuel Föcker; Alexander Cecil; Cornelia Prehn; Jerzy Adamski; Muriel Albrecht; Frederike Adams; Anke Hinney; Lars Libuda; Judith Bühlmeier; Johannes Hebebrand; Triinu Peters; Jochen Antel
Acute anorexia nervosa (AN) constitutes an extreme physiological state. We aimed to detect state related metabolic alterations during inpatient admission and upon short- and long-term weight regain. In addition, we tested the hypothesis that metabolite concentrations adapt to those of healthy controls (HC) after long-term weight regain. Thirty-five female adolescents with AN and 25 female HC were recruited
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Effect of a Single Bout of Aerobic Exercise on Kynurenine Pathway Metabolites and Inflammatory Markers in Prostate Cancer Patients—A Pilot Randomized Controlled Trial Metabolites (IF 4.097) Pub Date : 2020-12-23 Alexander Schenk; Tobias Esser; André Knoop; Mario Thevis; Jan Herden; Axel Heidenreich; Wilhelm Bloch; Niklas Joisten; Philipp Zimmer
The kynurenine (KYN) pathway gains growing research interest concerning the genesis, progression and therapy of solid tumors. Previous studies showed exercise-induced effects on metabolite levels along the KYN pathway. Modulations of the KYN pathway might be involved in the positive impact of exercise on prostate cancer progression and mortality. The objective of this trial was to investigate whether
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Liquid Chromatography-High-Resolution Mass Spectrometry-Based In Vitro Toxicometabolomics of the Synthetic Cathinones 4-MPD and 4-MEAP in Pooled Human Liver Microsomes Metabolites (IF 4.097) Pub Date : 2020-12-23 Sascha K. Manier; Florian Schwermer; Lea Wagmann; Niels Eckstein; Markus R. Meyer
Synthetic cathinones belong to the most often seized new psychoactive substances on an international level. This study investigated the toxicometabolomics, particularly the in vitro metabolism of 2-(methylamino)-1-(4-methylphenyl)-1-pentanone (4-MPD) and 2-(ethylamino)-1-(4-methylphenyl)-1-pentanone (4-MEAP) in pooled human liver microsomes (pHLM) using untargeted metabolomics techniques. Incubations
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Metabolomic Analysis to Elucidate Mechanisms of Sunitinib Resistance in Renal Cell Carcinoma Metabolites (IF 4.097) Pub Date : 2020-12-22 Tomonori Sato; Yoshihide Kawasaki; Masamitsu Maekawa; Shinya Takasaki; Kento Morozumi; Masahiko Sato; Shuichi Shimada; Naoki Kawamorita; Shinichi Yamashita; Koji Mitsuzuka; Nariyasu Mano; Akihiro Ito
Metabolomics analysis possibly identifies new therapeutic targets in treatment resistance by measuring changes in metabolites accompanying cancer progression. We previously conducted a global metabolomics (G-Met) study of renal cell carcinoma (RCC) and identified metabolites that may be involved in sunitinib resistance in RCC. Here, we aimed to elucidate possible mechanisms of sunitinib resistance
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Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles Metabolites (IF 4.097) Pub Date : 2020-12-22 Rofida Wahman; Andres Sauvêtre; Peter Schröder; Stefan Moser; Thomas Letzel
Plants produce a huge number of functionally and chemically different natural products that play an important role in linking the plant with the adjacent environment. Plants can also absorb and transform external organic compounds (xenobiotics). Currently there are only a few studies concerning the effects of xenobiotics and their transformation products on plant metabolites using a mass spectrometric
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Using Community Science to Reveal the Global Chemogeography of River Metabolomes Metabolites (IF 4.097) Pub Date : 2020-12-20 Vanessa A. Garayburu-Caruso; Robert E. Danczak; James C. Stegen; Lupita Renteria; Marcy Mccall; Amy E. Goldman; Rosalie K. Chu; Jason Toyoda; Charles T. Resch; Joshua M. Torgeson; Jacqueline Wells; Sarah Fansler; Swatantar Kumar; Emily B. Graham
River corridor metabolomes reflect organic matter (OM) processing that drives aquatic biogeochemical cycles. Recent work highlights the power of ultrahigh-resolution mass spectrometry for understanding metabolome composition and river corridor metabolism. However, there have been no studies on the global chemogeography of surface water and sediment metabolomes using ultrahigh-resolution techniques
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Evaluation of Saliva Stability for NMR Metabolomics: Collection and Handling Protocols Metabolites (IF 4.097) Pub Date : 2020-12-19 Daniela Duarte; Beatriz Castro; Joana Leonor Pereira; Joana Faria Marques; Ana Luísa Costa; Ana M. Gil
Maintaining a salivary metabolic profile upon sample collection and preparation is determinant in metabolomics. Nuclear magnetic resonance (NMR) spectroscopy was used to identify metabolite changes during short-term storage, at room temperature (RT)/4 °C/−20 °C, and after sample preparation, at RT/4 °C (mimicking typical clinical/laboratory settings). Interestingly, significant metabolic inter-individual