样式: 排序: IF: - GO 导出 标记为已读
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Recent structural discoveries of photosystems I and II acclimated to absorb far-red light BBA Bioenerg. (IF 4.3) Pub Date : 2024-02-22 Christopher J. Gisriel
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The mitochondrial respiratory chain from Rhodotorula mucilaginosa, an extremophile yeast BBA Bioenerg. (IF 4.3) Pub Date : 2024-02-13 Paulina Castañeda-Tamez, Natalia Chiquete-Félix, Salvador Uribe-Carvajal, Alfredo Cabrera-Orefice
survives extreme conditions through several mechanisms, among them its carotenoid production and its branched mitochondrial respiratory chain (RC). Here, the branched RC composition was analyzed by biochemical and complexome profiling approaches. Expression of the different RC components varied depending on the growth phase and the carbon source present in the medium. RC is constituted by all four
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Regulation of metabolism and proton motive force generation during mixed carbon fermentation by an Escherichia coli strain lacking the FOF1-ATPase BBA Bioenerg. (IF 4.3) Pub Date : 2024-02-12 Heghine Gevorgyan, Lilit Baghdasaryan, Karen Trchounian
Proton FF-ATPase is the key enzyme in under fermentative conditions. In this study the role of proton ATPase in the μ and formation of metabolic pathways during the fermentation of mixture of glucose, glycerol and formate using the DK8 (lacking FF) mutant strain was investigated. It was shown that the contribution of FF-ATPase in the specific growth rate was ~45 %. Formate was not taken up in the DK8
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Alternative Oxidase – Aid or obstacle to combat the rise of fungal pathogens? BBA Bioenerg. (IF 4.3) Pub Date : 2024-01-07 Elizabeth S.M. Edrich, Lucian Duvenage, Campbell W. Gourlay
Fungal pathogens present a growing threat to both humans and global health security alike. Increasing evidence of antifungal resistance in fungal populations that infect both humans and plant species has increased reliance on combination therapies and shown the need for new antifungal therapeutic targets to be investigated. Here, we review the roles of mitochondria and fungal respiration in pathogenesis
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Fluorescence quenching in aggregates of fucoxanthin–chlorophyll protein complexes: Interplay of fluorescing and dark states BBA Bioenerg. (IF 4.3) Pub Date : 2023-12-30 Andrius Gelzinis, Jevgenij Chmeliov, Marijonas Tutkus, Ernesta Vitulskienė, Marius Franckevičius, Claudia Büchel, Bruno Robert, Leonas Valkunas
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Performance of TMRM and Mitotrackers in mitochondrial morphofunctional analysis of primary human skin fibroblasts BBA Bioenerg. (IF 4.3) Pub Date : 2023-12-17 Shruti Desai, Sander Grefte, Els van de Westerlo, Susette Lauwen, Angela Paters, Jochen H.M. Prehn, Zhuohui Gan, Jaap Keijer, Merel J.W. Adjobo-Hermans, Werner J.H. Koopman
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Conformational changes in a Photosystem II hydrogen bond network stabilize the oxygen-evolving complex BBA Bioenerg. (IF 4.3) Pub Date : 2023-11-11 Brandon P. Russell, David J. Vinyard
The Mn4CaO5 oxygen-evolving complex (OEC) in Photosystem II (PSII) is assembled in situ and catalyzes water oxidation. After OEC assembly, the PsbO extrinsic subunit docks to the lumenal face of PSII and both stabilizes the OEC and facilitates efficient proton transfer to the lumen. D1 residue R334 is part of a hydrogen bond network involved in proton release during catalysis and interacts directly
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Oscillations of chlorophyll fluorescence after plasma membrane excitation in Chara originate from nonuniform composition of signaling metabolites in the streaming cytoplasm BBA Bioenerg. (IF 4.3) Pub Date : 2023-11-02 Alexander A. Bulychev, Tatiana S. Strelets
Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials (AP) whose regulatory influence on chlorophyll (Chl) fluorescence and photosynthesis extends over tens of minutes. Unlike plant leaves where the efficiency of photosystem II reaction (YII) undergoes a separate reversible depression after an individual AP, characean algae exhibit long-lasting
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Disturbed electron transport beyond PSI changes metabolome and transcriptome in Zn-deficient soybean BBA Bioenerg. (IF 4.3) Pub Date : 2023-10-16 Elisa Andresen, Filis Morina, Syed Nadeem Hussain Bokhari, Peter Koník, Hendrik Küpper
Low Zn availability in soils is a problem in many parts of the world, with tremendous consequences for food and feed production because Zn deficiency affects the yield and quality of plants. In this study we investigated the consequences of Zn-limitation in hydroponically cultivated soybean (Glycine max L.) plants. Parameters of photosynthesis biophysics were determined by spatially and spectrally
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The cyanobacterial FtsH4 protease controls accumulation of protein factors involved in the biogenesis of photosystem I BBA Bioenerg. (IF 4.3) Pub Date : 2023-10-11 Peter Koník, Petra Skotnicová, Sadanand Gupta, Martin Tichý, Surbhi Sharma, Josef Komenda, Roman Sobotka, Vendula Krynická
Membrane-bound FtsH proteases are universally present in prokaryotes and in mitochondria and chloroplasts of eukaryotic cells. These metalloproteases are often critical for viability and play both protease and chaperone roles to maintain cellular homeostasis. In contrast to most bacteria bearing a single ftsH gene, cyanobacteria typically possess four FtsH proteases (FtsH1–4) forming heteromeric (FtsH1/3
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Direct observation of triplet energy transfer between chlorophylls and carotenoids in the core antenna of photosystem I from Thermosynechococcus elongatus BBA Bioenerg. (IF 4.3) Pub Date : 2023-10-11 J. Alster, D. Bína, K. Charvátová, H. Lokstein, J. Pšenčík
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Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein BBA Bioenerg. (IF 4.3) Pub Date : 2023-09-21 Dmitry V. Zlenko, Elena A. Protasova, Georgy V. Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Phycobilisomes (PBSs) are giant water-soluble light-harvesting complexes of cyanobacteria and red algae, consisting of hundreds of phycobiliproteins precisely organized to deliver the energy of absorbed light to chlorophyll chromophores of the photosynthetic electron-transport chain. Quenching the excess of excitation energy is necessary for the photoprotection of photosynthetic apparatus. In cyanobacteria
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Acclimation to medium-level non-lethal iron limitation: Adjustment of electron flow around the PSII and metalloprotein expression in Trichodesmium erythraeum IMS101 BBA Bioenerg. (IF 4.3) Pub Date : 2023-09-23 Antonio Colussi, Syed Nadeem Hussain Bokhari, Ana Mijovilovich, Peter Koník, Hendrik Küpper
The aim of this study was to investigate how acclimation to medium-level, long-term, non-lethal iron limitation changes the electron flux around the Photosystem II of the oceanic diazotroph Trichodesmium erythraeum IMS101. Fe availability of about 5× and 100× lower than a replete level, i.e. conditions common in the natural environment of this cyanobacterium, were applied in chemostats. The response
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Tuning of the ChlD1 and ChlD2 properties in photosystem II by site-directed mutagenesis of neighbouring amino acids BBA Bioenerg. (IF 4.3) Pub Date : 2023-09-17 Miwa Sugiura, Masaya Kimura, Naohiro Shimamoto, Yuki Takegawa, Makoto Nakamura, Kazumi Koyama, Julien Sellés, Alain Boussac, A. William Rutherford
Photosystem II is the water/plastoquinone photo-oxidoreductase of photosynthesis. The photochemistry and catalysis occur in a quasi-symmetrical heterodimer, D1D2, that evolved from a homodimeric ancestor. Here, we studied site-directed mutants in PSII from the thermophilic cyanobacterium Thermosynechoccocus elongatus, focusing on the primary electron donor chlorophyll a in D1, ChlD1, and on its symmetrical
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Unravelling the fluorescence kinetics of light-harvesting proteins with simulated measurements BBA Bioenerg. (IF 4.3) Pub Date : 2023-09-11 Callum Gray, Lekshmi Kailas, Peter G. Adams, Christopher D.P. Duffy
The plant light-harvesting pigment-protein complex LHCII is the major antenna sub-unit of PSII and is generally (though not universally) accepted to play a role in photoprotective energy dissipation under high light conditions, a process known Non-Photochemical Quenching (NPQ). The underlying mechanisms of energy trapping and dissipation within LHCII are still debated. Various models have been proposed
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Spillover in the direct-type PSI-PSII megacomplex isolated from Arabidopsis thaliana is regulated by pH BBA Bioenerg. (IF 4.3) Pub Date : 2023-09-11 Makio Yokono, Chiyo Noda, Jun Minagawa
Various megacomplexes in which Photosystem I and Photosystem II are physically bound (PSI-PSII m.c.) have been found in many organisms. In terms of function, these can be divided into two groups: those in which PSII and PSI are closely coupled (direct-type, photoprotection), and those in which a large light-harvesting antenna is placed between PSII and PSI (bridged-type, energy sharing). Arabidopsis
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Optically detected magnetic resonance and mutational analysis reveal significant differences in the photochemistry and structure of chlorophyll f synthase and photosystem II BBA Bioenerg. (IF 4.3) Pub Date : 2023-08-09 Alessandro Agostini, Gaozhong Shen, Donald A. Bryant, John H. Golbeck, Art van der Est, Donatella Carbonera
In cyanobacteria that undergo far red light photoacclimation (FaRLiP), chlorophyll (Chl) f is produced by the ChlF synthase enzyme, probably by photo-oxidation of Chl a. The enzyme forms homodimeric complexes and the primary amino acid sequence of ChlF shows a high degree of homology with the D1 subunit of photosystem II (PSII). However, few details of the photochemistry of ChlF are known. The results
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Mitochondrial alternative oxidase across the tree of life: Presence, absence, and putative cases of lateral gene transfer BBA Bioenerg. (IF 4.3) Pub Date : 2023-08-07 Ryan J. Weaver, Allison E. McDonald
The alternative oxidase (AOX) is a terminal oxidase in the electron transport system that plays a role in mitochondrial bioenergetics. The past 20 years of research shows AOX has a wide yet patchy distribution across the tree of life. AOX has been suggested to have a role in stress tolerance, growth, and development in plants, but less is known about its function in other groups, including animals
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Heavy metals do not induce ROS production by mitochondrial respirasome BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-28 Jaime A. de Lira-Sánchez, Mercedes Esparza-Perusquía, Federico Martínez, Juan P. Pardo, Oscar Flores-Herrera
Since the discovery of the respirasome constituted by complexes I, III2, and IV, its precise participation in mitochondrial bioenergetics is poorly understood. We previously determined a higher NADH:DBQ oxidoreductase activity coupled to a lower ROS production by the respirasome than the free complex I. Toxicological studies suggest that respiratory complexes are heavy metals target during mitochondrial
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Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-30 I. Satoh, K. Gotou, S. Nagatsuma, K.V.P. Nagashima, M. Kobayashi, L.-J. Yu, M.T. Madigan, Y. Kimura, Z.-Y. Wang-Otomo
Phospholipid–protein interactions play important roles in regulating the function and morphology of photosynthetic membranes in purple phototrophic bacteria. Here, we characterize the phospholipid composition of intracytoplasmic membrane (ICM) from Rhodobacter (Rba.) sphaeroides that has been genetically altered to selectively express light-harvesting (LH) complexes. In the mutant strain (DP2) that
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A possible relationship between the effect of factors on photoactivation of photosystem II depleted of functional Mn and cytochrome b559 BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-26 Andrey Khorobrykh
The photoassembly of the Mn4CaO5 cluster in Mn-depleted photosystem II preparations (photoactivation) was studied under the influence of oxidants, reductants and pH. New data on the effect of these factors on the photoactivation yield are presented. The presence of the oxidant, ferricyanide, negatively affected the photoactivation yield over the entire concentration range studied (0–1 mM). In contrast
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The catalytic reaction of cytochrome c oxidase probed by in situ gas titrations and FTIR difference spectroscopy BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-27 Federico Baserga, Julian Storm, Ramona Schlesinger, Joachim Heberle, Sven T. Stripp
Cytochrome c oxidase (CcO) is a transmembrane heme‑copper metalloenzyme that catalyzes the reduction of O2 to H2O at the reducing end of the respiratory electron transport chain. To understand this reaction, we followed the conversion of CcO from Rhodobacter sphaeroides between several active-ready and carbon monoxide-inhibited states via attenuated total reflection Fourier-transform infrared (ATR
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How chloride functions to enable proton conduction in photosynthetic water oxidation: Time-resolved kinetics of intermediates (S-states) in vivo and bromide substitution BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-25 Colin Gates, Jonah M. Williams, Gennady Ananyev, G. Charles Dismukes
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The binding of light-harvesting antennae to PsaB suppresses the PSII to PSI spillover BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-09 Makio Yokono, Eunchul Kim, Jun Minagawa
Abstract not available
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Conserved tyrosine in phytochromes controls the photodynamics through steric demand and hydrogen bonding capabilities BBA Bioenerg. (IF 4.3) Pub Date : 2023-07-10 Tobias Fischer, Lisa Köhler, Philipp D. Engel, Chen Song, Wolfgang Gärtner, Josef Wachtveitl, Chavdar Slavov
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Far-red photosynthesis: Two charge separation pathways exist in plant Photosystem II reaction center BBA Bioenerg. (IF 4.3) Pub Date : 2023-06-23 Andrea Pavlou, Fredrik Mokvist, Stenbjörn Styring, Fikret Mamedov
An alternative charge separation pathway in Photosystem II under the far-red light was proposed by us on the basis of electron transfer properties at 295 K and 5 K. Here we extend these studies to the temperature range of 77–295 K with help of electron paramagnetic resonance spectroscopy. Induction of the S2 state multiline signal, oxidation of Cytochrome b559 and ChlorophyllZ was studied in Photosystem
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Tight association of CpcL with photosystem I in Anabaena sp. PCC 7120 grown under iron-deficient conditions BBA Bioenerg. (IF 4.3) Pub Date : 2023-06-13 Shota Shimizu, Haruya Ogawa, Naoki Tsuboshita, Takehiro Suzuki, Koji Kato, Yoshiki Nakajima, Naoshi Dohmae, Jian-Ren Shen, Ryo Nagao
Phycobilisomes (PBSs), which are huge pigment-protein complexes displaying distinctive color variations, bind to photosystem cores for excitation-energy transfer. It is known that isolation of supercomplexes consisting of PBSs and photosystem I (PSI) or PBSs and photosystem II is challenging due to weak interactions between PBSs and the photosystem cores. In this study, we succeeded in purifying PSI-monomer-PBS
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Three structures of PSI-LHCI from Chlamydomonas reinhardtii suggest a resting state re-activated by ferredoxin BBA Bioenerg. (IF 4.3) Pub Date : 2023-06-01 Christoph Gerle, Yuko Misumi, Akihiro Kawamoto, Hideaki Tanaka, Hisako Kubota-Kawai, Ryutaro Tokutsu, Eunchul Kim, Dror Chorev, Kazuhiro Abe, Carol V. Robinson, Kaoru Mitsuoka, Jun Minagawa, Genji Kurisu
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A bacteriorhodopsin-based biohybrid solar cell using carbon-based electrolyte and cathode components BBA Bioenerg. (IF 4.3) Pub Date : 2023-05-24 Christopher Espinoza-Araya, Ricardo Starbird, E. Senthil Prasad, Venkatesan Renugopalakrishnan, Ashok Mulchandani, Barry D. Bruce, Claudia C. Villarreal
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Role of pheophytin a in the primary charge separation of photosystem I from Acaryochloris marina: Femtosecond optical studies of excitation energy and electron transfer reactions BBA Bioenerg. (IF 4.3) Pub Date : 2023-05-13 Anastasia A. Petrova, Anna Paola Casazza, Ivan V. Shelaev, Fedor E. Gostev, Arseny V. Aybush, Victor A. Nadtochenko, Alexey Yu. Semenov, Stefano Santabarbara, Dmitry A. Cherepanov
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On the inter-monomer electron transfer in cytochrome bc1 BBA Bioenerg. (IF 4.3) Pub Date : 2023-05-08 Arkadiusz Borek, Robert Ekiert, Artur Osyczka
Cytochrome bc1 is a structural and functional homodimer. The catalytically-relevant inter-monomer electron transfer has been implicated by a number of experiments, including those based on analyses of the cross-dimer mutated derivatives. As some of the original data on these derivatives have recently been questioned, we extend kinetic analysis of these mutants to confirm the enzymatic origin of the
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Energy dissipation efficiency in the CP43 assembly intermediate complex of photosystem II BBA Bioenerg. (IF 4.3) Pub Date : 2023-05-03 Sandeep Biswas, Dariusz M. Niedzwiedzki, Himadri B. Pakrasi
Photosystem II in oxygenic organisms is a large membrane bound rapidly turning over pigment protein complex. During its biogenesis, multiple assembly intermediates are formed, including the CP43-preassembly complex (pCP43). To understand the energy transfer dynamics in pCP43, we first engineered a His-tagged version of the CP43 in a CP47-less strain of the cyanobacterium Synechocystis 6803. Isolated
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Exploring substrate interaction in respiratory alternative complex III from Rhodothermus marinus BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-29 Filipa Calisto, Smilja Todorovic, Ricardo O. Louro, Manuela M. Pereira
Rhodothermus marinus is a thermohalophilic organism that has optimized its microaerobic metabolism at 65 °C. We have been exploring its respiratory chain and observed the existence of a quinone:cytochrome c oxidoreductase complex, named Alternative Complex III, structurally different from the bc1 complex. In the present work, we took profit from nanodiscs and liposomes technology to investigate ACIII
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Enhanced function of non-photoinhibited photosystem II complexes upon PSII photoinhibition BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-25 Sanna Gunell, Tapio Lempiäinen, Eevi Rintamäki, Eva-Mari Aro, Mikko Tikkanen
Light induced photosystem (PS)II photoinhibition inactivates and irreversibly damages the reaction center protein(s) but the light harvesting complexes continue the collection of light energy. Here we addressed the consequences of such a situation on thylakoid light harvesting and electron transfer reactions. For this purpose, Arabidopsis thaliana leaves were subjected to investigation of the function
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Energetics and proton release in photosystem II from Thermosynechococcus elongatus with a D1 protein encoded by either the psbA2 or psbA3 gene BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-18 Alain Boussac, Julien Sellés, Miwa Sugiura
In the cyanobacterium Thermosynechococcus elongatus, there are three psbA genes coding for the Photosystem II (PSII) D1 subunit that interacts with most of the main cofactors involved in the electron transfers. Recently, the 3D crystal structures of both PsbA2-PSII and PsbA3-PSII have been solved [Nakajima et al., J. Biol. Chem. 298 (2022) 102668.]. It was proposed that the loss of one hydrogen bond
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Concerted primary proton transfer reactions in a thermophilic rhodopsin studied by time-resolved infrared spectroscopy at high temperature BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-18 Kunisato Kuroi, Takashi Tsukamoto, Naoya Honda, Yuki Sudo, Yuji Furutani
The primary proton transfer reactions of thermophilic rhodopsin, which was first discovered in an extreme thermophile, Thermus thermophilus JL-18, were investigated using time-resolved Fourier transform infrared spectroscopy at various temperatures ranging from 298 to 343 K (25 to 70 °C) and proton transport activity analysis. The analyses were performed using counterion (D95E, D95N, D229E, and D229N)
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Two separate pathways underlie NADH and succinate oxidation in swine heart mitochondria: Kinetic evidence on the mobile electron carriers BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-12 Salvatore Nesci, Cristina Algieri, Fabiana Trombetti, Micaela Fabbri, Giorgio Lenaz
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Primary charge separation in native and plant pheophytin a-modified reaction centers of Chloroflexus aurantiacus: Ultrafast transient absorption measurements at low temperature BBA Bioenerg. (IF 4.3) Pub Date : 2023-04-14 Alexey A. Zabelin, Anton M. Khristin, Vyacheslav B. Kovalev, Ravil A. Khatypov, Anatoly Ya. Shkuropatov
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Effects of acute cigarette smoke concentrate exposure on mitochondrial energy transfer in fast- and slow-twitch skeletal muscle BBA Bioenerg. (IF 4.3) Pub Date : 2023-03-25 Stephen T. Decker, Nadia Alexandrou-Majaj, Gwenael Layec
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Solar water splitting Pt-nanoparticle photosystem I thylakoid systems: Catalyst identification, location and oligomeric structure BBA Bioenerg. (IF 4.3) Pub Date : 2023-03-29 Lisa M. Utschig, Nestor J. Zaluzec, Tirupathi Malavath, Nina S. Ponomarenko, David M. Tiede
Photosynthetic conversion of light energy into chemical energy occurs in sheet-like membrane-bound compartments called thylakoids and is mediated by large integral membrane protein-pigment complexes called reaction centers (RCs). Oxygenic photosynthesis of higher plants, cyanobacteria and algae requires the symbiotic linking of two RCs, photosystem II (PSII) and photosystem I (PSI), to split water
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Conversion of light into electricity in a semi-synthetic system based on photosynthetic bacterial chromatophores BBA Bioenerg. (IF 4.3) Pub Date : 2023-03-29 Liya A. Vitukhnovskaya, Andrey A. Zaspa, Alexey Yu. Semenov, Mahir D. Mamedov
Chromatophores (Chr) from photosynthetic nonsulfur purple bacterium Rhodobacter sphaeroides immobilized onto a Millipore membrane filter (MF) and sandwiched between two semiconductor indium tin oxide (ITO) electrodes (termed ITO|Chr – MF|ITO) have been used to measure voltage (ΔV) induced by continuous illumination. The maximum ΔV was detected in the presence of ascorbate / N,N,N’N'-tetramethyl-p-phenylenediamine
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Proton-translocating NADH:ubiquinone oxidoreductase of Paracoccus denitrificans plasma membranes catalyzes FMN-independent reverse electron transfer to hexaammineruthenium (III) BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-24 Grigory V. Gladyshev, Tatyana V. Zharova, Alexandra V. Kareyeva, Vera G. Grivennikova
NADH-OH, the specific inhibitor of NADH-binding site of the mammalian complex I, is shown to completely block FMN-dependent reactions of P. denitrificans enzyme in plasma membrane vesicles: NADH oxidation (in a competitive manner with Ki of 1 nM) as well as reduction of pyridine nucleotides, ferricyanide and oxygen in the reverse electron transfer. In contrast to these activities, the reverse electron
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Mutational analysis of a conserved positive charge in the c-ring of E. coli ATP synthase BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-21 Rashmi K. Shrestha, Michael W. Founds, Sam J. Shepard, Mallory M. Rothrock, Amy E. Defnet, P. Ryan Steed
F1Fo ATP synthase is a ubiquitous molecular motor that utilizes a rotary mechanism to synthesize adenosine triphosphate (ATP), the fundamental energy currency of life. The membrane-embedded Fo motor converts the electrochemical gradient of protons into rotation, which is then used to drive the conformational changes in the soluble F1 motor that catalyze ATP synthesis. In E. coli, the Fo motor is composed
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Disruption of mitochondrial bioenergetics and calcium homeostasis by phytanic acid in the heart: Potential relevance for the cardiomyopathy in Refsum disease BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-20 Ângela Beatriz Zemniaçak, Ana Cristina Roginski, Rafael Teixeira Ribeiro, Julia Gabrieli Bender, Rafael Aguiar Marschner, Simone Magagnin Wajner, Moacir Wajner, Alexandre Umpierrez Amaral
Refsum disease is an inherited peroxisomal disorder caused by severe deficiency of phytanoyl-CoA hydroxylase activity. Affected patients develop severe cardiomyopathy of poorly known pathogenesis that may lead to a fatal outcome. Since phytanic acid (Phyt) concentrations are highly increased in tissues of individuals with this disease, it is conceivable that this branched-chain fatty acid is cardiotoxic
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In vitro reversible dehydration in C3-substituents of zinc chlorophyll analogs by BchF and BchV enzymes: Stereoselectivity and substrate specificity in the dehydration BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-22 Mitsuaki Hirose, Yusuke Tsukatani, Jiro Harada, Hitoshi Tamiaki
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Chiral-phase HPLC separation of (divinyl-)protochlorophyllide-a enantiomers as key precursors in chlorophyll biosynthesis from their 132-stereoisomeric prime forms BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-21 Mai Shiozaki, Tadashi Mizoguchi, Jiro Harada, Mitsuaki Hirose, Hitoshi Tamiaki
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The protein family of pyruvate:quinone oxidoreductases: Amino acid sequence conservation and taxonomic distribution BBA Bioenerg. (IF 4.3) Pub Date : 2023-02-07 Filipe M. Sousa, Bárbara Fernandes, Manuela M. Pereira
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Mass spectrometry and spectroscopic characterization of a tetrameric photosystem I supercomplex from Leptolyngbya ohadii, a desiccation-tolerant cyanobacterium BBA Bioenerg. (IF 4.3) Pub Date : 2023-01-25 Dariusz M. Niedzwiedzki, Nikki Cecil M. Magdaong, Xinyang Su, Noam Adir, Nir Keren, Haijun Liu
Cyanobacteria inhabiting desert biological soil crusts face the harsh conditions of the desert. They evolved a suite of strategies toward desiccation-hydration cycles mixed with high light irradiations, etc. In this study we purified and characterized the structure and function of Photosystem I (PSI) from Leptolyngbya ohadii, a desiccation-tolerant desert cyanobacterium. We discovered that PSI forms
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Photoinduced electron transfer in cytochrome bc1: Dynamics of rotation of the Iron-sulfur protein during bifurcated electron transfer from ubiquinol to cytochrome c1 and cytochrome bL BBA Bioenerg. (IF 4.3) Pub Date : 2023-01-26 Jeffrey Havens, Ting Su, Qiyu Wang, Chang-An Yu, Linda Yu, Bill Durham, Francis Millett
The electron transfer reactions within wild-type Rhodobacter sphaeroides cytochrome bc1 (cyt bc1) were studied using a binuclear ruthenium complex to rapidly photooxidize cyt c1. When cyt c1, the iron‑sulfur center Fe2S2, and cyt bH were reduced before the reaction, photooxidation of cyt c1 led to electron transfer from Fe2S2 to cyt c1 with a rate constant of ka = 80,000 s−1, followed by bifurcated
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Calcium-bound structure of bovine cytochrome c oxidase BBA Bioenerg. (IF 4.3) Pub Date : 2023-01-25 Kazumasa Muramoto, Kyoko Shinzawa-Itoh
The crystal structure of bovine cytochrome c oxidase (CcO) shows a sodium ion (Na+) bound to the surface of subunit I. Changes in the absorption spectrum of heme a caused by calcium ions (Ca2+) are detected as small red shifts, and inhibition of enzymatic activity under low turnover conditions is observed by addition of Ca2+ in a competitive manner with Na+. In this study, we determined the crystal
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Production, homology modeling and mutagenesis studies on GlcH glucose transporter from Prochlorococcus sp. strain SS120 BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-20 José Ángel Moreno-Cabezuelo, María del Carmen Muñoz-Marín, Antonio López-Lozano, Diogo Athayde, Ana Simón-García, Jesús Díez, Margarida Archer, Federico M. Issoglio, José Manuel García-Fernández
The marine cyanobacterium Prochlorococcus is one of the main primary producers on Earth, which can take up glucose by using the high affinity, multiphasic transporter GlcH. We report here the overexpression of glcH from Prochlorococcus marinus strain SS120 in Escherichia coli. Modeling studies of GlcH using the homologous MelB melibiose transporter from Salmonella enterica serovar Typhimurium showed
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Isolation of a novel heterodimeric PSII complex via strep-tagged PsbO BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-23 Jan Lambertz, Jakob Meier-Credo, Svetlana Kucher, Enrica Bordignon, Julian D. Langer, Marc M. Nowaczyk
The multi-subunit membrane protein complex photosystem II (PSII) catalyzes the light-driven oxidation of water and with this the initial step of photosynthetic electron transport in plants, algae, and cyanobacteria. Its biogenesis is coordinated by a network of auxiliary proteins that facilitate the stepwise assembly of individual subunits and cofactors, forming various intermediate complexes until
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pH-dependent kinetics of NO release from E. coli bd-I and bd-II oxidase reveals involvement of Asp/Glu58B BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-16 Iryna Makarchuk, Jan Kägi, Tatjana Gerasimova, Daniel Wohlwend, Thorsten Friedrich, Frédéric Melin, Petra Hellwig
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Unveiling the membrane bound dihydroorotate: Quinone oxidoreductase from Staphylococcus aureus BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-06 Filipe M. Sousa, Patrícia Pires, Andreia Barreto, Patrícia N. Refojo, Micael S. Silva, Pedro B. Fernandes, Ana P. Carapeto, Tiago T. Robalo, Mário S. Rodrigues, Mariana G. Pinho, Eurico J. Cabrita, Manuela M. Pereira
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Xenotopic expression of alternative oxidase (AOX) to study mechanisms of mitochondrial disease BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-06 Carlo Viscomi, Anthony L. Moore, Massimo Zeviani, Marten Szibor
The mitochondrial respiratory chain or electron transport chain (ETC) facilitates redox reactions which ultimately lead to the reduction of oxygen to water (respiration). Energy released by this process is used to establish a proton electrochemical gradient which drives ATP formation (oxidative phosphorylation, OXPHOS). It also plays an important role in vital processes beyond ATP formation and cellular
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Tunnel dynamics of quinone derivatives and its coupling to protein conformational rearrangements in respiratory complex I BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-09 Jonathan Lasham, Outi Haapanen, Volker Zickermann, Vivek Sharma
Respiratory complex I in mitochondria and bacteria catalyzes the transfer of electrons from NADH to quinone (Q). The free energy available from the reaction is used to pump protons and to establish a membrane proton electrochemical gradient, which drives ATP synthesis. Even though several high-resolution structures of complex I have been resolved, how Q reduction is linked with proton pumping, remains
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Deletion of the ATP20 gene in Ustilago maydis produces an unstable dimer of F1FO-ATP synthase associated with a decrease in mitochondrial ATP synthesis and a high H2O2 production BBA Bioenerg. (IF 4.3) Pub Date : 2022-12-09 Mercedes Esparza-Perusquía, Thorsten Langner, Giovanni García-Cruz, Michael Feldbrügge, Guadalupe Zavala, Juan Pablo Pardo, Federico Martínez, Oscar Flores-Herrera
The F1FO-ATP synthase uses the energy stored in the electrochemical proton gradient to synthesize ATP. This complex is found in the inner mitochondrial membrane as a monomer and dimer. The dimer shows higher ATPase activity than the monomer and is essential for cristae folding. The monomer-monomer interface is constituted by subunits a, i/j, e, g, and k. The role of the subunit g in a strict respiratory