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  • Green synthesis of silver nanoparticles from Curcuma longa L. and coating on the cotton fabric for antimicrobial applications and wound healing activity
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-25
    M. Maghimaa; Sulaiman Ali Alharbi

    The cotton fabrics are a cosmopolitan in usage due to their extraordinary features. The clothes are a very good medium for the growth of pathogenic microorganisms. The nanoparticles have diverse benefits in the biomedical field like drug carrier and as antimicrobials. The current investigation was aimed to synthesize the metallic silver nanoparticles (AgNPs) from the aqueous extract of Curcuma longa leaf and evaluating their antimicrobial and wound healing potential of AgNPs coated cotton fabric. The synthesized AgNPs were characterized by HR-TEM and FT-IR examinations. The formulated AgNPs were coated with cotton fabric to test their efficiency against the pathogenic microorganisms. The existence of AgNPs in the cotton fabric was confirmed via the SEM along with EDX analysis. The antimicrobial potential of fabricated AgNPs and its coated cotton fabric was inspected against the human pathogenic strains. The wound healing efficacy was examined in the L929 cells. The HR-TEM analysis proved the existence of spherical shaped AgNPs. In the antimicrobial activity, the CL-AgNPs loaded cotton fabric was exhibited an appreciable decrease in the growth of pathogenic microorganisms. The crude extract, as well as formulated AgNPs, also exhibited the noticeable antimicrobial potency against the S.aureus, P.aeruginosa, S.pyogenes, and C.albicans. The AgNPs loaded cotton fabric was displayed the potent wound healing activity in the fibroblast (L929) cells. Consequently, it was concluded that the formulated AgNPs from C.longa coated cotton fabrics may be utilized for the variety of applications in hospital patients and even medical workers to prevent the microbial infection.

    更新日期:2020-01-26
  • Photodynamic therapy mediated by aluminium-phthalocyanine nanoemulsion eliminates primary tumors and pulmonary metastases in a murine 4T1 breast adenocarcinoma model
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-25
    Mosar Corrêa Rodrigues; Lívia Gumieri Vieira; Frederíco Hillesheim Horst; Eduarda Campos de Araújo; Rayane Ganassin; Carolin Merker; Thomas Meyer; Julia Böttner; Tom Venus; João Paulo F. Longo; Sacha Braun Chaves; Mônica Pereira Garcia; Irina Estrela-Lopis; Ricardo Bentes Azevedo; Luis Alexandre Muehlmann

    Photodynamic therapy (PDT) is effective in the treatment of different types of cancer, such as basal cell carcinoma and other superficial cancers. However, improvements in photosensitizer delivery are still needed, and the use of PDT against more deeply located tumors has been the subject of many studies. Thus, the goal of this study was to evaluate the efficacy of a nanoemulsion containing aluminium-phthalocyanine (AlPc-NE) as a mediator of photodynamic therapy (PDT-AlPc-NE) against grafted 4T1 breast adenocarcinoma tumors in mice (BALB/c). Short after the appearance of the tumor, the animals were divided into groups (n = 5) as follows: untreated; only AlPc-NE and treated with PDT-AlPc-NE. The tumor volume was measured with a digital calliper at specific times. The presence of metastasis in the lungs was evaluated by microtomography and histopathological analyses. The results show that the application of PDT-AlPc-NE eradicated the transplanted tumors in all the treated animals, while the animals from control groups presented a robust increase in the tumor volume. Still more significantly, microtomography showed the animals submitted the PDT-AlPc-NE to be free of detectable metastasis in the lungs. The histological analysis of the lungs further confirmed the results verified by the microtomography. Therefore, this study suggests that PDT-AlPc-NE is effective in the elimination of experimentally grafted breast tumors in mice and also in preventing the formation of metastasis in the lungs.

    更新日期:2020-01-26
  • 更新日期:2020-01-26
  • 更新日期:2020-01-26
  • 更新日期:2020-01-24
  • Tribenzoporphyrazines with dendrimeric peripheral substituents and their promising photocytotoxic activity against Staphylococcus aureus
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-20
    Dariusz T. Mlynarczyk; Jolanta Dlugaszewska; Michal Falkowski; Lukasz Popenda; Michal Kryjewski; Wojciech Szczolko; Stefan Jurga; Jadwiga Mielcarek; Tomasz Goslinski
    更新日期:2020-01-21
  • Protein-stabilized gold nanoclusters for PDT: ROS and singlet oxygen generation
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-20
    Vilius Poderys; Greta Jarockyte; Saulius Bagdonas; Vitalijus Karabanovas; Ricardas Rotomskis
    更新日期:2020-01-21
  • Surface modified glass substrate for sensing E. coli using highly stable and luminescent CdSe/CdS core shell quantum dots
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-20
    Chandan Hunsur Ravikumar; R. Shwetharani; R. Geetha Balakrishna

    CdSe/CdS core shelled quantum dots (QDs) were prepared by colloidal synthesis using a binary ligand system and a non-coordinating, reusable solvent n-octadecane (nOD). Both the synthesis of CdSe and CdSe/CdS core shelled quantum dots were achieved by hot injection technique at much lower temperatures than reported earlier. The use of binary ligand facilitated enough nucleation and growth. Red shift in absorption spectra, an enhanced crystallite and particle size is evidenced by XRD and TEM respectively, confirming the formation of core shell structure of CdSe/CdS. The synthesized core shells exhibited high fluorescence intensity, long term stability and good mono dispersion, making it a potential material for bio-imaging and sensing. Core shell QDs were modified with mercapto propionic acid (MPA) to impart aqueous solubility. Studies on cytotoxicity of shelled QDs reveal good bio compatibility with a very minimum toxicity of IC50 = 20 μg/L. These QDs were used for sensing E. coli. Ordinary glass slide, modified using plasma etching is surface modified through APTES aiding conjugation of antibodies. Anti- E. coli polyclonal antibody on glass matrix (slide) and antibody conjugated QDs were used for detection of E. coli in a typical sandwich model. The excellent optical transparency of glass and high emission of QDs lead to detection of E.coli with a limit of detection of 50 CFU/mL.

    更新日期:2020-01-21
  • One step preparation of stable gold nanoparticle using red cabbage extracts under UV light and its catalytic activity
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-18
    Ilay Sema Unal; Ayse Demirbas; Irem Onal; Nilay Ildiz; Ismail Ocsoy
    更新日期:2020-01-21
  • Detection of prostate cancer by Raman spectroscopy: A multivariate study on patients with normal and altered PSA values
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-18
    Neandder A. Correia; Lucas T.A. Batista; Roberto J.M. Nascimento; Maria C.T. Cangussú; Pedro J.L. Crugeira; Luiz G.P. Soares; Landulfo Silveira; Antonio L.B. Pinheiro
    更新日期:2020-01-21
  • Effect of blue light at 410 and 455 nm on Pseudomonas aeruginosa biofilm
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-17
    Eleonora Martegani; Fabrizio Bolognese; Nicola Trivellin; Viviana Teresa Orlandi

    Pseudomonas aeruginosa is an opportunistic pathogen resistant to many antibiotics, able to form biofilm and causes serious nosocomial infections. Among anti-Pseudomonas light-based approaches, the recent antimicrobial Blue Light (aBL) treatment seems very promising. The aim of this study was to evaluate the efficiency of blue light in inhibiting and/or eradicating P. aeruginosa biofilm. Light at 410 nm has been identified as successful in inhibiting biofilm formation not only of the model strain PAO1, but also of CAUTI (catheter-associated urinary tract infection) isolates characterized by their ability to form biofilm. Results of this work on 410 nm light also demonstrated that: i) at the lowest tested radiant exposure (75 J cm−2) prevents matrix formation; ii) higher radiant exposures (225 and 450 J cm−2) light impairs the cellular components of biofilm, adherent and planktonic ones; iii) light eradicates with a good rate young and older biofilms in a light dose dependent manner; iv) it is also efficient in inactivating catalase A, a virulence factor playing an important role in pathogenic mechanism. Light at 455 nm, even if at a lower extent than 410 nm, showed a certain anti-Pseudomonas activity. Furthermore, light at 410 nm caused detrimental effects on enzyme activity of β-galactosidase and catalase A, and changes on plasmid DNA conformation and ortho-nitrophenyl-β-D-galactopyranoside structure. This study supports the potential of blue light for anti-infective and disinfection applications.

    更新日期:2020-01-17
  • Photobiomodulation and the expression of genes related to the JAK/STAT signalling pathway in wounded and diabetic wounded cells
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-17
    Sandy Winfield Jere; Nicolette Nadene Houreld; Heidi Abrahamse

    Photobiomodulation therapy (PBMT) is a curative technique that uses low intensity light to relegate pain and inflammation, and accelerate tissue repair. At a molecular level, the effects of photobiomodulation (PBM) are not fully established. The present study aimed to assess the impact of PBM on the alteration of genes linked to Janus kinase-Signal transducer and activator of transcription (JAK-STAT) signalling in wounded and diabetic wounded cells in vitro. Cells were irradiated using a diode laser at a wavelength of 660 nm and an energy density of 5 J/cm2. RNA was extracted from cells 48 h post-irradiation, and was used to synthesise complementary deoxyribonucleic acid (cDNA) that was used in PCR arrays to profile for 84 JAK/STAT signalling related genes. Irradiation at a wavelength of 660 nm and an energy density of 5 J/cm2 significantly regulated genes related to the JAK/STAT signalling pathway in wounded and diabetic wounded cells. In irradiated wounded cells, 19 genes were significantly regulated, of which two were up-regulated and 17 were down-regulated, while 73 genes were significantly regulated in irradiated diabetic wounded cells of which 46 were up-regulated and 27 were down-regulated. This data suggests that PBM modulates gene transcription for protein synthesis and activates cellular signalling, and may indeed be helpful in enhancing diabetic wound repair.

    更新日期:2020-01-17
  • 更新日期:2020-01-15
  • 更新日期:2020-01-13
  • Recovery of inferior alveolar nerve by photobiomodulation therapy using two laser wavelengths: A behavioral and immunological study in rat
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-10
    Neda Hakimiha; Mohammad Mehdi Dehghan; Homa Manaheji; Jalal Zaringhalam; Saeed Farzad Mohajeri; Reza Fekrazad; Neda Moslemi

    Postoperative sensory disturbances of inferior alveolar nerve (IAN) are major challenges in dental procedures. We aimed to investigate the effect of photobiomodulation therapy (PBMT) with 810 nm and 980 nm diode lasers on behavioral and immunological factors in a rat IAN crush model. Seventy-two rats were randomly assigned to the four groups of 810 nm laser (crush injury+810 nm laser; 6 J/cm2, 15 sessions, every 48 h), 980 nm laser (crush injury+980 nm laser; same protocol), control (crush injury without irradiation), and sham surgery (no crush injury and no irradiation). The neurosensory response of IAN was evaluated by Von Frey behavioral test before (baseline) and post-surgery in a period of one month. Changes of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), nuclear factor-kappa B (NF-κB), TNF-α, and IL-1β, were assessed on days 2 and 30 post injury. Data were analyzed for significant differences by repeated measures and one-way ANOVA (p < .05). One day after surgery, all rats subjected to nerve injury showed significant increase in the withdrawal threshold of von Frey test compared to the baseline (p = .02 for control and p = .03 for laser groups). The threshold gradually returned to the baseline scores in 810 nm, 980 nm, and control groups from days 11, 17, and 29, respectively. There was a significant lower withdrawal threshold in 810 nm and 980 nm laser groups compared to the control group in days 11 to 19 and 9 to 23, respectively. At both time points, the levels of NGF and BDNF were significantly higher in 810 nm laser group compared to the control group. There was a significant difference between laser and control groups regarding NF-κB expression (all p values<.001). TNF-α and IL-1β were significantly lower in laser groups compared to the control group (all p values < .001). PBMT with 810 and 980 nm diode laser protocol used in this study, promoted the neurosensory recovery of IAN after crush injury in rats. In addition, application of 810 nm diode laser was associated with more improvement in immunological responses compared to that of 980 nm laser.

    更新日期:2020-01-10
  • Silver nanoparticles synthesis from Bacillus sp KFU36 and its anticancer effect in breast cancer MCF-7 cells via induction of apoptotic mechanism
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-10
    Mohammed A. Almalki; Ashraf Y.Z. Khalifa

    Biological synthesis of nanoparticles is a growing research trend because it has numerous applications in pharmaceutics and biomedicine. The aim of this study was to obtain silver nanoparticles (AgNPs) from Bacillus sp. KFU36, a marine strain, and to assess its anticancer activity. The supernatant of Bacillus sp. KFU36 was supplemented with silver nitrate and the nanoparticles obtained were characterized spectrophotometrically and microscopically. A band of surface plasmon resonance was appeared at 430 nm, as revealed by UV–vis spectrophotometry. X-ray diffraction spectrum and Energy Dispersive Spectroscopy confirmed the crystalline and metallic structure of the AgNPs, respectively. Scanning electron microscopy revealed that the shape of the synthesized AgNPs were spherical and the size extended between 5 and 15 nm. The AgNPs were investigated for their potential anticancer effects on the cell viability, migration and apoptosis using MTT and wound-healing assays, and flow cytometry, respectively. The cytotoxic effects of these nanoparticles were evidenced by the decreasing the cell viability (as 15% at 50 μg/ml), cell density, adhesion capacity and losing the normal shape and size, and inducing the apoptosis on MCF-7 by 61% at 50 μg/mL. These findings confirm that the synthesized AgNPs exhibited superior anticancer activities and therefore could be exploited as a promising, cost-effective, and environmentally benign strategy in treating this disease in future.

    更新日期:2020-01-10
  • Green synthesis of iron oxide nanorods using Withania Coagulans extract improved photocatalytic degradation and antimicrobial activity
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-10
    Shaheen Qasim; Ayesha Zafar; Muhammad Saqib Saif; Zeshan Ali; Maryem Nazar; Muhammad Waqas; Ain Ul Haq; Tuba Tariq; Shahbaz Gul Hassan; Faisal Iqbal; Xu-Gang Shu; Murtaza Hasan

    Present work compares the green synthesis of iron oxide nanorodes (NRs) using Withania coagulans and reduction precipitation based chemical method. UV/Vis confirmed the Sharp peak of Iron oxide NRs synthesized by biologically and chemically on 294 and 278 nm respectively. XRD and SEM showed highly crystalline nature of NRs with average size 16 ± 2 nm using Withania extract and less crystalline with amorphous Nanostructure of 18 ± 2 nm by chemical method. FTIR analysis revealed the involvement of active bioreducing and stabilizing biomolecules in Withania coagulans extract for synthesis of NRs. Moreover, EDX analysis indicates 34.91% of Iron oxide formation in biological synthesis whereas 25.8% of iron oxide synthesis in chemical method. The degradation of safranin dye in the presence of Withania Coagulans based NRs showed 30% more effectively than chemically synthesized Nanorods which were verified by the gradual decrease in the peak intensity at 553 nm and 550 nm respectively under solar irradiation. Furthermore, Withania Coagulans based NRs showed effective Antibacterial activity against S.aureus and P. aeuroginosa as compared to NRs by chemical method. Finally, we conclude that green synthesized NRs are more effective and functionally more efficient than chemically prepared NRs. Therefore, our work will help the researchers to boost the synthesis of nanoparticles via biological at commercial level.

    更新日期:2020-01-10
  • Synthesis, characterization and evaluation of visible light active cadmium sulfide-graphitic carbon nitride nanocomposite: A prospective solar light harvesting photo-catalyst for the deactivation of waterborne pathogen
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-08
    Umair Baig; Adil Hawsawi; M.A. Ansari; M.A. Gondal; M.A. Dastageer; W.S. Falath

    Cadmium sulfide-graphitic carbon nitride nanocomposite was synthesized by pulsed laser ablation in liquid, and it was found from the results of optical and morphological characterizations that the proper anchoring of nanostructured cadmium sulfide on the nano-sheets of graphitic carbon nitride took place, which brought about the positive attributes such as enhanced visible light absorption and reduced photo-generated charge recombination, the key features required for an efficient photo-catalyst by solar light harvesting. The pulsed laser ablation in liquid method adopted for the synthesis of cadmium sulfide-graphitic carbon nitride has the following advantages: the shape and size of the synthesized particles can be controlled by altering the experimental parameters such as laser wavelength, pulse laser duration, the pH of the solution, the surfactants and the temperature of the solution, pulsed laser ablation in liquid method neither requires cumbersome equipment nor does it require intermediate chemicals and catalysts nor does it necessitate the post synthesis purification. The enhancement of photo-catalytic activity of cadmium sulfide-graphitic carbon nitride nanocomposite was tested for the photo-catalytic deactivation of Escherichia coli bacteria in water under visible light radiation. As anticipated, a significant improvement of photo-catalytic deactivation was observed, which is attributed to the enhanced and extended light absorption in the visible spectral region, and the formation of herterojunction between the semiconductors, which is instrumental in inhibiting the undesired recombination of photo-generated charge carriers. Quantitatively, the presence of cadmium sulfide on the graphitic carbon nitride surface contributed to a remarkable 129% increase of photo-catalytic degradation constant compared to pure graphitic carbon nitride, which resulted in the decrease of total depletion time of Escherichia coli from 156 min to 67 min with the cadmium sulfide-graphitic carbon nitride nanocomposite synthesized by pulsed laser ablation in liquid method. Our results on the efficient photo-catalytic deactivation of Escherichia coli under visible light assures that cadmium sulfide-graphitic carbon nitride nanocomposite can very well be used for photo-catalytic water purification by harvesting the abundant solar light.

    更新日期:2020-01-08
  • Targeted photoimmunotherapy based on photosensitizer-antibody conjugates for multiple myeloma treatment
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Wael M. Darwish; Noha A. Bayoumi; Hanan M. El-Shershaby; Nasr M. Allahloubi
    更新日期:2020-01-07
  • N-dihydrogalactochitosan-supported tumor control by photothermal therapy and photothermal therapy-generated vaccine
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Mladen Korbelik; Judit Banáth; Wei Zhang; Tomas Hode; Samuel S.K. Lam; Paul Gallagher; Jianhua Zhao; Haishan Zeng; Wei R. Chen

    Photothermal therapy (PTT) is recently clinically established cancer therapy that uses near-infrared light for thermal ablation of solid tumors. The biopolymer N-dihydrogalactochitosan (GC) was shown in multiple reports to act as a very effective adjunct to tumor PTT. In the present study, mouse tumor model SCCVII (squamous cell carcinoma) was used with two protocols, in situ tumor PTT and therapeutic PTT vaccine for tumors, for investigating the effects of GC. The results reveal that GC can potentiate tumoricidal action of PTT through both direct and indirect mechanisms. In addition to previously known capacity of GC for activating immune effector cells, the indirect means is shown to include reducing the populations of immunoregulatory T cells (Tregs) in PTT-treated tumors. Testing the effects of GC on PTT-treated SCCVII tumor cells in vitro uncovered the existence of a direct mechanism evident by reduced colony survival of these cells. Fluorescence microscopy demonstrated increased binding of fluorescein-labeled GC to PTT-treated compared to untreated SCCVII cells that can be blocked by pre-exposure to annexin V. The results of additional in vitro testing with specific inhibitors demonstrate that these direct mechanisms do not involve the engagement of death surface receptors that trigger extrinsic apoptosis pathway signaling but may be linked to pro-survival activity of caspase-1. Based on the latter, it can be suggested that GC-promoted killing of PTT-treated cells stems from interference of GC bound to damaged membrane components with the repair of these structures that consequently hinders cell survival.

    更新日期:2020-01-07
  • Size-dependent apoptotic activity of gold nanoparticles on osteosarcoma cells correlated with SERS signal
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Avisekh Chakraborty; Ankita Das; Sreyan Raha; Ananya Barui

    In the last decade, gold nanoparticles have emerged as promising agents for in vitro bio-sensing and in vivo cancer theranostics. However, different investigations have reported widely varying cytotoxicity and uptake efficiency of gold nanoparticles depending upon their size. Therefore, more extensive studies are needed to standardize these biological effects as a function of size on a particular cell line. In addition, to obtain robust confirmation on the correlation of a size to biological effect, thorough mechanistic study must also be performed. In this study, the size dependent biological activities of gold nanoparticles on osteosarcoma cells is investigated towards exploring their potential theranostic application in bone cancer, for which very scarce literature reports are available. Tris-assisted citrate based method was optimized to synthesize stable gold naoparticles of 40–60 nm sizes. Nanoparticles were characterized through UV–Vis spectroscopy, field emission scanning electron microscope (FESEM) and dynamic light scattering (DLS). Increasing concentrations of gold nanoparticles (AuNPs) of 46 nm size, enhanced the rate of reactive oxygen species (ROS)-induced apoptosis in MG63 cells by disrupting their mitochondrial membrane potential. Considerably higher cell death was observed for 46 and 60 nm AuNPs compared to 38 nm at all concentrations of 200, 400 and 800 ng/ml. Further, molecular signatures of cellular apoptosis under nanoparticle treatment were optically assessed through surface enhanced Raman scattering (SERS). A significant Raman enhancement in cancer cells under treatment of larger gold nanoparticles (46 and 60 nm) at fixed wavelength of 785 nm and laser power of 8.0 mW was evident. In corroboration with molecular biology techniques, SERS observation confirmed the size-dependent apoptotic phenomena in osteosarcoma cells under treatment of gold nanoparticles. Study demonstrates a facile, non-active targeting approach for detection of size-dependent AuNP-induced apoptosis in osteosarcoma cells through label-free SERS method.

    更新日期:2020-01-07
  • Improved charge separation through H2O2 assisted copper tungstate for enhanced photocatalytic efficiency for the degradation of organic dyes under simulated sun light
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Mathew Waimbo; Gabriel Anduwan; Ora Renagi; Srikanth Badhula; Kenney Michael; Jongwoo Park; Senthilkumar Velusamy; Yong Soo Kim
    更新日期:2020-01-07
  • PacCOP1 negatively regulates anthocyanin biosynthesis in sweet cherry (Prunus avium L.)
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Dong Liang; Tingting Zhu; Qunxian Deng; Lijin Lin; Yi Tang; Jin Wang; Xun Wang; Xian Luo; Huifen Zhang; Xiulan Lv; Hui Xia

    Light is a key environmental factors affecting anthocyanin accumulation in plants. Ubiquitin E3 ligase COP1 has been proved to be a negative regulator involved in light-regulated plant development process, whereas the function and expression specificity of COP1 in anthocyanin biosynthesis in sweet cherry remains unclear. In the present study, we identified a COP1 in sweet cherry, named PacCOP1, it exhibited apparent different expression patterns in red-colored ‘Hongdeng’ and bi-colored ‘Satonishiki’, with increasing trend largely in ‘Satonishiki’, but decreasing trend in ‘Hongdeng’ after veraison, which was contrary to their variation tendency of anthocyanin content. While the expression abundance of anthocyanin biosynthesis related genes were largely increased after veraison, in accordance with anthocyanin content. Correlation analysis proved that the expression of PacCOP1 was negative correlated with the major genes on anthocyanin accumulation in ‘Hongdeng’ and ‘Satonishiki’ fruit, in especial PacDFR, PacANS, PacMYBA and PacbHLH33. Furthermore, over-expression of PacCOP1 in Arabidopsis displayed increased COP1 transcript level with negligible pigmentation and corresponding lower expression level of AtPAP1, AtDFR, AtLDOX, and AtUFGT. These results revealed the negative regular role of PacCOP1 in anthocyanin biosynthesis by repressing the PacMYBA transcription level, followed by down-regulating the structural genes expression abundance, eventually leading to attenuated anthocyanin accumulation in fruits.

    更新日期:2020-01-07
  • Development of thermosensitive hydrogel containing methylene blue for topical antimicrobial photodynamic therapy
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-07
    Brian Leung; Priyanga Dharmaratne; Wei Yan; Ben C.L. Chan; Clara B.S. Lau; Kwok-Pui Fung; Margaret Ip; Sharon S.Y. Leung
    更新日期:2020-01-07
  • Neuroprotective effect of Wogonin on Rat's brain exposed to gamma irradiation
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-05
    Liying Wang; Chenyu Li; Nagaraja Sreeharsha; Anurag Mishra; Vipin Shrotriya; Ajay Sharma

    Wogonin (5,7-dihydroxy-8-methoxy flavone), an active component isolated from the root of Scutellaria baicalensis Georgi. Neurotoxic effects of γ irradiation have been established in humans and animals. The current study was designed to evaluate whether wogonin could restrain γ irradiation-induced neurotoxicity in rats and to explore the underlying mechanisms. Rats were divided into five groups, 10 rats each. Group 1 was orally administered distilled water and served as control. Group 2 received an oral daily dose of wogonin (30 mg/kg). Rats in group 3 were exposed to a whole-body single dose of γ-irradiation. Animals in group 4 received an oral daily dose of wogonin (30 mg/kg) for 15 days then exposed to a whole-body single dose of γ-irradiation. In group 5, rats were exposed to a whole-body single dose of γ-irradiation then were orally administered a daily dose of wogonin (30 mg/kg) for 15 days. There were significant increases in malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and Interleukin 6 (IL-6) levels and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) mRNA and protein expression. Whereas significant decreases in reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) level as well as nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) mRNA and protein expression in the irradiated group when compared with the relevant control. The cerebral cortex of irradiated rats showed vacuolization and nuclear pyknosis in the neuronal cells and focal gliosis. Wogonin administration pre- or post-irradiation significantly ameliorated all these previous effects. Wogonin had antioxidant and anti-inflammatory effects and ameliorated the histopathological changes in the brain.

    更新日期:2020-01-06
  • 更新日期:2020-01-04
  • Photolysis of thiochrome in aqueous solution: A kinetic study
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-03
    Zubair Anwar; Muhammad Ali Sheraz; Sofia Ahmed; Nafeesa Mustaan; Adeela Khurshid; Wajiha Gul; Saif-Ur-Rehman Khattak; Iqbal Ahmad
    更新日期:2020-01-04
  • Apoptotic induction and anti-metastatic activity of eugenol encapsulated chitosan nanopolymer on rat glioma C6 cells via alleviating the MMP signaling pathway
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2020-01-02
    Zhenjiang Li; Vishnu Priya Veeraraghavan; Surapaneni Krishna Mohan; Srinivasa Rao Bolla; Hariprasath Lakshmanan; Subramanian Kumaran; Wilson Aruni; Aref Ali Mohammed Aladresi; Omar H.M. Shair; Sulaiman Ali Alharbi; Arunachalam Chinnathambi

    Glioma is the prime cause of cancer allied mortality in adolescent people and it accounts about 80% of all malignant tumours. Eugenol is a major bioactive constituent present in the essential oils with numerous pharmacological benefits including nueroprotective activity. The major drawback of eugenol is its extreme volatile property and oxygen sensitivity therefore we increased the efficacy of drug; eugenol by encapsulating with chitosan polymer. Eugenol loaded chitosan polymer (EuCs) was characterized using FTIR, XRD, SEM, HR-TEM analysis and the encapsulation, drug release efficacy was assessed at in vitro condition. The induction of autophagy and anticancer efficacy of EuCs on glioma cells was evaluated with rat C6 glioma cells using MTT assay, acridine orange staining, immunocytochemical analysis of NFκβ protein expression and FLOW cytometric analysis. The anti-metastatic property of Eu-CS was assessed by immunoblotting and RT-PCR analysis of epithelial mesenchymal transition protein expression in EuCs treated rat C6 glioma cells. Our characterization analysis proves that EuCs possess essential physical and functional properties of copolymer to be utilized as a drug. Further the MTT analysis and AO staining confirms even in the presence of oncogenic inducer and autophagic inhibitors, EuCs exhibits apoptotic potency on rat C6 glioma cells. The result of immunocytochemical studies depicts the inhibition of NFκβ protein expression and flow cytometry studies confirm apoptosis induction by EuCs. The inhibition of metastasis by EuCs was proven by the decrease in epithelial mesenchymal transition protein expression in Eu-Cs treated rat C6 glioma cells. Over all our results authentically confirms eugenol loaded chitosan nanopolymer persuasively induces apoptosis and inhibits metastasis in rat C6 glioma cells.

    更新日期:2020-01-02
  • The influence of light quality on the production of bioactive metabolites – Verbascoside, isoverbascoside and phenolic acids and the content of photosynthetic pigments in biomass of Verbena officinalis L. cultured in vitro
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-30
    Paweł Kubica; Agnieszka Szopa; Barbara Prokopiuk; Łukasz Komsta; Bożena Pawłowska; Halina Ekiert

    In vitro callus cultures of Verbena officinalis L. were maintained on solid Murashige and Skoog medium, enriched with 1 mg dm−3 BA and 1 mg dm−3 IBA under LED lights (red, blue, red/blue 70%/30%) in darkness and under control fluorescent lamps. The measurements of 2 phenylpropanoid glycosides (verbascoside and isoverbascoside) and 23 phenolic acids were performed in methanolic extracts from the biomass collected after 2-, 3- and 4-week growth cycles using the HPLC-DAD method. The presence of verbascoside, isoverbascoside and additionaly 7 phenolic acids (protocatechuic, chlorogenic, vanillic, caffeic, ferulic, o-coumaric and m-coumaric acids) was confirmed in all extracts. Blue and red/blue lights stimulated the accumulation of verbascoside (max. of 6716 and 6023 mg 100 g−1 DW after a 4-week growth cycle) and isoverbascoside (max. 333 and 379 mg 100 g−1 DW also after 4 weeks). The maximum amounts of verbascoside and isoverbascoside were respectively 1.8- and 7.0-fold higher than under the control conditions. Phenolic acids were accumulated in different amounts, and the maximum total amounts ranged from 33 to 65 mg 100 g−1 DW. LED lights also stimulated their accumulation in comparison with darkness and control. The main phenolic acids included: m-coumaric acid (max. 39 mg 100 g−1 DW), ferulic acid (max. 12 mg 100 g−1 DW), and protocatechuic acid (max. 13 mg 100 g−1 DW). Additionally, the quantities of photosynthetic pigments (chlorophyll a, b and carotenoids) were estimated in acetonic extracts using spectrophotometry. Red/blue light stimulated the biosynthesis of pigments (max. Total content 287 μg g−1 FW after 4-week growth cycles). This is the first study describing the effect of LED lights on the production of phenylpropanoid glycosides and phenolic acids in V. officinalis callus cultures. Very high amounts of verbascoside and isoverbascoside are interesting from a practical point of view.

    更新日期:2019-12-30
  • Spectroscopic insight into the interaction of dopamine with spherical gold nanoparticles
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-30
    Janmejaya Rout; Bikash Chandra Swain; Padmaja P. Mishra; Umakanta Tripathy
    更新日期:2019-12-30
  • 更新日期:2019-12-30
  • Abilities of protocatechuic acid and its alkyl esters, ethyl and heptyl protocatechuates, to counteract UVB-induced oxidative injuries and photoaging in fibroblasts L929 cell line
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-30
    Regina G. Daré; Mariana M. Oliveira; Maria C.T. Truiti; Celso V. Nakamura; Valdecir F. Ximenes; Sueli O.S. Lautenschlager
    更新日期:2019-12-30
  • Hesperidin loaded Zn2+@ SA/PCT nanocomposites inhibit the proliferation and induces the apoptosis in colon cancer cells (HCT116) through the enhancement of pro-apoptotic protein expressions
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-28
    Zhen Yang; Hongwei Yang; Xinhua Dong; Minglong Pu; Feihong Ji

    Colon carcinoma is a recurring type of cancer that affects the intestine epithelial with a poor survival rate. It was already proven the anticancer property of hesperidin in various cancers but the bioavailability hesperidin is poor, which hinders the hesperidin usage. In this investigation we synthesized hesperidin loaded Zn2+@ SA/PCT nanocomposites and assessed its anticancer potential against colon cancer (HCT116) cells. Hesperidin loaded Zn2+@ SA/PCT nanocomposites were characterized using Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis. The drug releasing capacity and cytotoxic property was assessed via drug releasing assay, MTT assay with HCT116 cells. The anticancer potency of hesperidin nanocomposites were evaluated with TUNEL, DAPI staining, reactive oxygen species (ROS) generation assay and it is confirmed with flow cytometry analysis of MMP disruption in colon cancer (HCT116) cell line. Further the immunoblotting analysis of cysteine proteases Caspases 3, 9, PARP, proapoptotic protein Bax and antiapoptotic protein Bcl2 were performed. The results of FTIR, XRD and electroscopic analyses confirmed the synthesized hesperidin nanocomposites accomplish the properties of potent nanodrug and the MTT assay authentically confirmed that the synthesized hesperidin nanocomposite inhibited the HCT116 cell growth, and the results of fluorescent staining proved that the hesperidin nanocomposite induced the apoptotic mediated cell necrosis via promoting the expression of apoptotic proteins thereby induced the apoptosis in colon cancer (HCT116) cells. Hence, it was concluded that the, hesperidin loaded nanocomposites persuasively inhibited proliferation of colon carcinoma cell and induced apoptosis in in vitro condition.

    更新日期:2019-12-29
  • 更新日期:2019-12-25
  • Blue and red light affects morphogenesis and 20-hydroxyecdisone content of in vitro Pfaffia glomerata accessions
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-24
    Tatiane Dulcineia Silva; Diego Silva Batista; Evandro Alexandre Fortini; Kamila Motta de Castro; Sérgio Heitor Sousa Felipe; Amanda Mendes Fernandes; Raysa Mayara de Jesus Sousa; Kristhiano Chagas; José Victor Siqueira da Silva; Ludmila Nayara De Freitas Correia; Letícia Monteiro Farias; João Paulo Viana Leite; Diego Ismael Rocha; Wagner Campos Otoni
    更新日期:2019-12-25
  • Antibiofilm effect of mesoporous titania coatings on Pseudomonas aeruginosa biofilms
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-24
    Magdalena Pezzoni; Paolo N. Catalano; Diana C. Delgado; Ramón Pizarro; Martín G. Bellino; Cristina S. Costa
    更新日期:2019-12-25
  • Photodamage on Staphylococcus aureus by natural extract from Tetragonia tetragonoides (Pall.) Kuntze: Clean method of extraction, characterization and photophysical studies
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-24
    Katieli da Silva Souza Campanholi; Jonas Marcelo Jaski; Ranulfo Combuca da Silva Junior; Ana Beatriz Zanqui; Danielle Lazarin-Bidóia; Claudia Marques da Silva; Edson Antonio da Silva; Noboru Hioka; Celso Vataru Nakamura; Lucio Cardozo-Filho; Wilker Caetano
    更新日期:2019-12-25
  • 更新日期:2019-12-25
  • Synthesis, characterization and photocatalytic dye degradation capability of Calliandra haematocephala-mediated zinc oxide nanoflowers
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-18
    Ramesh Vinayagam; Raja Selvaraj; Arivalagan Pugazhendhi; Thivaharan Varadavenkatesan
    更新日期:2019-12-19
  • Anticancer and genotoxicity effect of (Clausena lansium (Lour.) Skeels) Peel ZnONPs on neuroblastoma (SH-SY5Y) cells through the modulation of autophagy mechanism
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-17
    Fu Li; Li Song; Xiaomei Yang; Zhiwei Huang; Xiao Mou; Asad Syed; Ali H. Bahkali; Libo Zheng

    Nanotechnology is an emerged field to develop the plant mediated metal based nanodrugs by green method. In this current study, the zinc oxide metal based nanoparticles were developed using (Clausena lansium (Lour.) Skeels) Peel aqueous extracts and zinc nitrate. The C.L extract zinc nanoparticleswere indicated by the sharp peak seen at 350 nm utilizing the Ultraviolet–Visible spectroscopy (UV–Vis). The high peaks indicate the presence ofphytochemicals and its functional groups in ZnONPs were studied by the Fourier Transform Infrared Spectroscopy (FT-IR). The X-Ray Diffraction analysis (XRD) explores the pattern and structure of ZnONPs as spherical and base-centered monoclinic crystalline shapes. The C.L extract with Zn nanoparticles were spherical in nature and the size of the synthesized particles were about 28.42 nm respectively. The autophagy (Beclin-1, LC3-I, LC3-II and ATG4B) and apoptotic (Bax, Bcl-2 and Caspase-3) proteins were regulated by the treatment with ZnONPs in SH-SY5Y neuroblastoma cells. The DNA loss or damage was occurred in the ZnONPs treatment and it was performed using Comet assay. The ZnONPs treatment generates the ROS in the cells and decreased its stability and viability. Addition of NAC prevents ROS in the cultured SH-SY5Y cells and prevents the cells from the apoptosis. We concluded that the ZnONPs potentially kills the neuroblastoma cells by producing the intracellular ROS.

    更新日期:2019-12-18
  • A novel method to detect bovine sex pheromones using l-tyrosine-capped silver nanoparticles: Special reference to nanosensor based estrus detection
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-17
    Chidhambaram Manikkaraja; Shahid Mahboob; Khalid A. Al-Ghanim; Durairaj Rajesh; Kumaresan Selvaraj; Muthusamy Sivakumar; Fahad A.M. Al-Misned; Zubair Ahmed; Govindaraju Archunan
    更新日期:2019-12-18
  • Biosynthesis of gold nanoparticles using Vetex negundo and evaluation of pro-apoptotic effect on human gastric cancer cell lines
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-17
    Zhou Yun; Arunachalam Chinnathambi; Sulaiman Ali Alharbi; Zhu Jin

    Gastric cancer (GC) is mainly widespread gastrointestinal malignancy,which reports for 8% of overallcases in carcinogenesis and 10% of yearly fatality, is 4thprimary cause of cancer associated death global. The plan of the present research was to develop ethanolic extract of Vitex negundo-loaded gold nanoparticles (VN-AuNPs) and to appraise the various characteristic methods likes UV–vis spectroscopy, SAED, FTIR, XRD and HR-TEM. Additionally, the anticancer effect of VN-AuNPs on AGS cells were analysed by cell viability, apoptotic morphological changes by TUNEL, AO/EtBr and Hoechst staining, alterations of mitochondrial membrane potential (MMP) and production reactive oxygen species (ROS). Moreover, the status of apoptosis gene such as caspase-3, Bcl-2, Bcl-XL, Bax and caspase-9 expressions was analysed by using western and RT-PCR techniques. Synthesized AuNPs established by UV absorption peak of the highest at 538 and crystal nature of AuNPs was additionallyverifiedwith SAED and XRD. TEM images were illustrates size and morphological division of NPs. FTIR examinationscompletedalkene, carbodiimide and aliphatic primary amines of biomolecules werepresent in synthesized VN-AuNPs. Additionally, AuNPs were stimulatedapoptosis throughthe cytotoxicity effect,changes of MMP, generation of ROS, nuclear and apoptotic morphological alterationsvia TUNEL, AO/EtBr and Hoechst assay. Furthermore, molecular mechanisms also provoked apoptosis through modulating pro (caspase-3, Bax, Bid, caspase-9) and anti-apoptotic (Bcl-2 and Bcl-XL) mediators by western blotting and gene expression in AGS cells. This production of AuNPs from VN was eco-friendly, large-scaled up and easy.

    更新日期:2019-12-18
  • Nd:YAP laser in the treatment of dentinal hypersensitivity: An ex vivo study.
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-13
    Carlo Fornaini; Nathalie Brulat-Bouchard; Etienne Medioni; Shiying Zhang; Jean-Paul Rocca; Elisabetta Merigo

    Purpose: The aims of this ex vivo study were to evaluate the effectiveness of the Nd:YAP laser in the treatment of dentin hypersensitivity, to compare the temperature rise during laser irradiation at three different dentine thicknesses, and to analyse the composition of the dentine-lased surface. Methods: A total of 33 teeth were used in this study. For scanning electron microscopy (SEM) observation, 24 teeth were transversely sectioned and divided into 4 groups: group A was irrigated with EDTA; group B was irradiated by Nd:YAP laser with 180 mJ energy/per pulse, 0.9 W average power, and 5 Hz frequency (power density [PD] = 229 W/cm2); group C was irradiated by Nd:YAP laser with 280 mJ energy/pulse, 1.4 W average power, and 5 Hz frequency (PD = 356 W/cm2); and group D was irradiated by Nd:YAP with 360 mJ energy/pulse, 1.8 W average power, and 5 Hz frequency (PD = 458 W/cm2). Energy-dispersive spectroscopy (EDS) analysis was performed on the same teeth evaluated for SEM observations. For temperature increase evaluation performed with thermocouples, 9 teeth were transversely sectioned at 3 different thicknesses (3 for each group) of 1, 2, and 3 mm. Results: Statistical analysis showed significant changes in the diameter of the dentinal tubule orifices among all groups; EDS did not show modification of the Ca/P ratio. Temperature increase under irradiation exceeded 5.5 °C only in the group D samples. Conclusions: This ex vivo study, based on temperature recording, SEM observation, and EDS analysis, demonstrated that Nd:YAP laser at a PD of 356 W/cm2, corresponding to an average power of 1.4 W, defines the best treatment for dentine hypersensitivity in terms of compromise between efficacy of the treatment and safety of the pulp.

    更新日期:2019-12-13
  • Zinc oxide‑selenium heterojunction composite: Synthesis, characterization and photo-induced antibacterial activity under visible light irradiation
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-13
    Aftab Ahmad; Sadeeq Ullah; Waqas Ahmad; Qipeng Yuan; Raheela Taj; Arif Ullah Khan; Aziz Ur Rahman; Usman Ali Khan
    更新日期:2019-12-13
  • Dopaminergic induction of human dental pulp stem cells by photobiomodulation: comparison of 660nm laser light and polychromatic light in the nir
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-12
    Merve Çapkın Yurtsever; Arlin Kiremitçi; Menemşe Gümüşderelioğlu

    Human dental pulp stem cells (hDPSCs) are able to differentiate into dopaminergic neurons and help the maintenance of partially degenerated neurons, which makes them as an alternative cell source for treatment of Parkinsons' disease (PD) patients. Here, the effect of photobiomodulation with polychromatic light source in the near infrared (NIR) range (600–1200 nm) or low level 660 nm diode laser light on hDPSCs during dopaminergic induction was investigated. Real time RT-qPCR analysis indicated that expressions of brain derived neurotrophic factor (BDNF), glial cell line derived neurotropic factor (GNDF), matrix associated protein 2 (MAP2), nuclear receptor related 1 protein (NURR1) and dopamine transporter (DAT) were increased, especially in the first 7 days of dopaminergic induction when 660 nm laser light was applied with a total energy density of 1.6 J/cm2. The activity of polychromatic light on hDPSCs depended on the differentiation media and protein type. BDNF, GDNF, NURR-1 and MAP2 expressions were increased in the presence of pre-induction factors, and decreased when the post-induction factors were added into the culture medium. In contrast with all these promising results, the dopaminergically induced hDPSCs did not show any functional characteristics of dopaminergic neurons and died after they were transferred to a new laminin coated culture plates. In conclusion, the expression of dopaminergic neuron protective protein mRNAs in hDPSCs was increased by photobiomodulation in defined conditions. However, the cells were not able to differentiate into functional dopaminergic neurons either in control or in photobiomodulated groups that are prone to cell death and exhibit immature dopaminergic neuron characteristics.

    更新日期:2019-12-13
  • Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-12
    Luciana Lourenço Ribeiro Vitor; Mariel Tavares Oliveira Prado; Natalino Lourenço Neto; Rodrigo Cardoso Oliveira; Vivien Thiemy Sakai; Carlos Ferreira Santos; Thiago José Dionísio; Daniela Rios; Thiago Cruvinel; Thais Marchini Oliveira; Maria Aparecida Andrade Moreira Machado

    This study aimed to compare the synthesis and secretion of VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, and FGF-2 between pulp fibroblasts from human primary teeth (HPF) and stem cell from human deciduous teeth (SHED) before and after photobiomodulation. HPF were obtained from explant technique and characterized by immunohistochemistry, while SHED were obtained from digestion technique and characterized by flow cytometry. HPF (control group) and SHED were plated, let to adhere, and put on serum starvation to synchronize the cell cycles prior to photobiomodulation. Then, both cell lineages were irradiated with 660-nm laser according to the following groups: 2.5 and 3.7 J/cm2. MTT and crystal violet assays respectively verified viability and proliferation. ELISA Multiplex Assay assessed the following proteins: VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, FGF-2, at 6, 12, and 24 h after photobiomodulation, in supernatant and lysate. Two-way ANOVA/Tukey test evaluated cell viability and proliferation, while angiogenic production and secretion values were analyzed by one-way ANOVA (P < .05). Statistically similar HPF and SHED viability and proliferation patterns occurred before and after photobiomodulation (P > .05). HPF exhibited statistically greater values of all angiogenic proteins than did SHED, at all study periods, except for FGF-2 (supernatant; 12 h); VEGFR1 (lysate; non-irradiated; 12 h); and VEGFR1 (lysate; non-irradiated; 24 h). Photobiomodulation changed the synthesis and secretion of angiogenic proteins by HPF. HPF produced and secreted greater values of all tested angiogenic proteins than did SHED before and after irradiation with both energy densities of 2.5 and 3.7 J/cm2.

    更新日期:2019-12-13
  • Combined X-ray radiotherapy and laser photothermal therapy of melanoma cancer cells using dual-sensitization of platinum nanoparticles
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-10
    F. Daneshvar, F. Salehi, M. Karimi, R. Dehdari Vais, M.A. Mosleh-Shirazi, N. Sattarahmady

    Metal nanostructures are promising agents sensitizing by laser light and X-ray in photothermal therapy (PTT) and radiotherapy (RT) of cancer that improve treatment strategies of cancer. Nanoscale platinum materials are favorable in nanomedicine applications. In this study, platinum nanoparticles (PtNPs) were synthesized and applied for cancer therapy upon 808-nm laser light and X-ray radiation, or their combination. Two power densities of laser (1.0 and 1.5 W cm−2) and three X-ray doses (2, 4 and 6 Gy) were selected for irradiation of B16/F10 cell line at 24 and 72 h-post treatment. The synthesized PtNPs had a spherical shape with a diameter of 12.2 ± 0.7 nm, and were cytocompatible up to 250 μg mL−1. A photothermal conversion activity in a concentration-dependent manner at 72 h-post treatment was observed. Also, PtNPs represented cytotoxicity upon X-ray radiation doses of 2, 4, and 6 Gy after 24 h, while, 72-h time passing led to deeper outcomes. Dual radiation of laser light and X-ray into PtNPs considerably improved the treatment via reactive oxygen species (ROS) production. PtNPs can act as a novel dual absorber of laser light and X-ray, a common sensitizer, for treatment of cancer. The results of this study can be considered after further clinical investigations for treatment of tumor models.

    更新日期:2019-12-11
  • Theoretical study on cyclophane amide molecular receptors and its complexation behavior with TCNQ
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-09
    Meenakshi Sundaram Soma Sundaram, Selvam Karthick, Krishnamurty Sailaja, Rajendran Karkuzhali, Gopalakrishnan Gopu
    更新日期:2019-12-11
  • 更新日期:2019-12-07
  • 更新日期:2019-12-07
  • Imaging mitochondria and plasma membrane in live cells using solvatochromic styrylpyridines
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-05
    Tarushyam Mukherjee, Aravintha Siva, Komal Bajaj, Virupakshi Soppina, Sriram Kanvah
    更新日期:2019-12-05
  • Low-level laser therapy attenuates lung inflammation and airway remodeling in a murine model of idiopathic pulmonary fibrosis: Relevance to cytokines secretion from lung structural cells
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-04
    Auriléia Aparecida de Brito, Elaine Cristina da Silveira, Nicole Cristine Rigonato-Oliveira, Stephanie Souza Soares, Maysa Alves Rodrigues Brandao-Rangel, Clariana Rodrigues Soares, Tawany Gonçalves Santos, Cintia Estefano Alves, Karine Zanella Herculano, Rodolfo Paula Vieira, Adriana Lino-dos-Santos-Franco, Regiane Albertini, Flavio Aimbire, Ana Paula de Oliveira

    Idiopathic pulmonary fibrosis (IPF) is a progressive and chronic inflammatory disease with a poor prognosis and very few available treatment options. Low-level laser therapy (LLLT) has been gaining prominence as a new and effective anti-inflammatory and immunomodulatory agent. Can lung inflammation and the airway remodeling be regulated by LLLT in an experimental model of IPF in C57Bl/6 mice? The present study investigated if laser attenuates cellular migration to the lungs, the airway remodeling as well as pro-fibrotic cytokines secretion from type II pneumocytes and fibroblasts. Mice were irradiated (780 nm and 30 mW) and then euthanized fifteen days after bleomycin-induced lung fibrosis. Lung inflammation and airway remodeling were evaluated through leukocyte counting in bronchoalveolar lavage fluid (BALF) and analysis of collagen in lung, respectively. Inflammatory cells in blood were also measured. For in vitro assays, bleomycin-activated fibroblasts and type II pneumocytes were irradiated with laser. The pro- and anti-inflammatory cytokines level in BALF as well as cells supernatant were measured by ELISA, and the TGFβ in lung was evaluated by flow cytometry. Lung histology was used to analyze collagen fibers around the airways. LLLT reduced both migration of inflammatory cells and deposition of collagen fibers in the lungs. In addition, LLLT downregulated pro-inflammatory cytokines and upregulated the IL-10 secretion from fibroblasts and pneumocytes. Laser therapy greatly reduced total lung TGFβ. Systemically, LLLT also reduced the inflammatory cells counted in blood. There is no statistical difference in inflammatory parameters studied between mice of the basal group and the laser-treated mice. Data obtained indicate that laser effectively attenuates the lung inflammation, and the airway remodeling in experimental pulmonary fibrosis is driven to restore the balance between the pro- and anti-inflammatory cytokines in lung and inhibit the pro-fibrotic cytokines secretion from fibroblasts.

    更新日期:2019-12-04
  • Synthesis of core/shell of Co3O4@ZrO2 nanoparticle and investigation of antimicrobial potential and photocatalytic degradation of Rhodamine B
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-03
    Rajasree Shanmuganathan, Felix LewisOscar, Sabarathinam Shanmugam, Nooruddin Thajuddin, Sulaiman Ali Alharbi, Naiyf S. Alharbi, Kathirvel Brindhadevi, Arivalagan Pugazhendhi
    更新日期:2019-12-04
  • Looking into dental pulp stem cells in the therapy of photoreceptors and retinal degenerative disorders
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-03
    Hiba Amer Alsaeedi, Chenshen Lam, Avin Ee-Hwan Koh, Seoh Wei Teh, Pooi Ling Mok, Akon Higuchi, Kong Yong Then, Mae-Lynn Catherine Bastion, Badr Alzahrani, Aisha Farhana, Bala Sundaram Muthuvenkatachalam, Antony V. Samrot, K.B. Swamy, Najat Marraiki, Abdullah M. Elgorban, Suresh Kumar Subbiah

    Blindness and vision impairment are caused by irremediable retinal degeneration in affected individuals worldwide. Cell therapy for a retinal replacement can potentially rescue their vision, specifically for those who lost the light sensing photoreceptors in the eye. As such, well-characterized retinal cells are required for the replacement purposes. Stem cell-based therapy in photoreceptor and retinal pigment epithelium transplantation is well received, however, the drawbacks of retinal transplantation is the limited clinical protocols development, insufficient number of transplanted cells for recovery, the selection of potential stem cell sources that can be differentiated into the target cells, and the ability of cells to migrate to the host tissue. Dental pulp stem cells (DPSC) belong to a subset of mesenchymal stem cells, and are recently being studied due to its high capability of differentiating into cells of the neuronal lineage. In this review, we look into the potential uses of DPSC in treating retinal degeneration, and also the current data supporting its application.

    更新日期:2019-12-04
  • Opposite effects of low intensity light of different wavelengths on the planarian regeneration rate
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-03
    A.M. Ermakov, O.N. Ermakova, A.L. Popov, A.A. Manokhin, V.K. Ivanov

    Planarian freshwater flatworms have the unique ability to regenerate due to stem cell activity. The process of regeneration is extremely sensitive to various factors, including light radiation. Here, the effect of low-intensity LED light of different wavelengths on regeneration, stem cell proliferation and gene expression associated with these processes was studied. LED matrices with different wavelengths (red (λmax = 635 nm), green (λmax = 520 nm) and blue (λmax = 463 nm), as well as LED laser diodes (red (λmax = 638.5 nm), green (λmax = 533 nm) and blue (λmax = 420 nm), were used in the experiments. Computer-assisted morphometry, whole-mount immunocytochemical study and RT-PCR were used to analyze the biological effects of LED light exposure on the planarian regeneration in vivo. It was found that a one-time exposure of regenerating planarians with low-intensity red light diodes stimulated head blastema growth in a dose-dependent manner (up to 40%). The green light exposure of planarians resulted in the opposite effect, showing a reduced head blastema growth rate by up to 21%. The blue light exposure did not lead to any changes in the rate of head blastema growth. The maximum effects of light exposure were observed at a dose of 175.2 mJ/cm2. No significant differences were revealed in the dynamics of neoblasts' (planarian stem cells) proliferation under red and green light exposure. However, the RT-PCR gene expression analysis of 46 wound-induced genes revealed their up-regulation upon red LED light exposure, and down-regulation upon green light exposure. Thus, we have demonstrated that the planarian regeneration process is rather sensitive to the effects of low-intensity light radiation of certain wavelengths, the biological activity of red and green light being dictated by the different expression of the genes regulating transcriptional activity.

    更新日期:2019-12-04
  • Enhanced anti-lung carcinoma and anti-biofilm activity of fungal molecules mediated biogenic zinc oxide nanoparticles conjugated with β-D-glucan from barley
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-12-03
    Kandasamy Saravanakumar, Elango Jeevithan, Xiaowen Hu, Ramachandran Chelliah, Deog-Hwan Oh, Myeong-Hyeon Wang
    更新日期:2019-12-04
  • Photoresponse to different lighting strategies during red leaf lettuce growth
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-11-30
    Giedrė Samuolienė, Akvilė Viršilė, Perttu Haimi, Jurga Miliauskienė

    The objective of the study was to investigate the effects of growth-stage specific lighting for the physiological homeostasis of red leaf lettuce (Lactuca sativa L. cv. Red Cos), by measuring the productivity of photosynthesis and primary metabolism. In the experiments, the main photosynthetic photon flux consisted of red (R) and blue (B) light, supplemented with blue, green (G) or UV-A wavelengths. Decrease of fructose, accompanied by significant decrease of stomatal conductance (gs), the ratio of intracellular to ambient CO2 concentration (Ci/Ca), photosynthetic rate (Pr), light adapted actual quantum yield of PSII photochemistry (ΦPSII), biomass formation and significant increase of transpiration rate (Tr) suggest that supplemental UV-A during maturity stage, after supplemental green irradiation during seedling stage (BRG to BRUV) was the least favourable condition for red leaf lettuce. However, constant irradiation with supplemental green (BRG) or supplemental green irradiation after increased blue exposure (B↑R to BRG) resulted in significant increase of Pr, gs, Ci/Ca, and light use efficiency(LUE), and decrease of Tr and Water use efficiency (WUE). Significant increase of leaf area was observed under supplemental green in both seedlings (BR; BRG) and matured plants (B↑R to BRG). Significant increase of specific leaf area was found under supplemental green (BRG) for seedlings and under increased blue (B↑R) for matured plants. Accordingly, the most favourable growth-stage specific lighting spectrum strategy for red leaf lettuce, based on photosynthetic and primary metabolite response, is supplemental green irradiation after increased blue exposure (B↑R to BRG), whereas, the most favourable condition for seedlings is BRG. According to the PCA correlation matrix, associations among the measured data indicate that WUE negatively correlated with gs and Ci/Ca, while LUE positively correlated with gs and Pr. However, weak correlations between ФPSII, LUE and photochemical reflectance index (PRI) suggest that selected light conditions were not optimal for red leaf lettuce.

    更新日期:2019-11-30
  • Tetra-cationic platinum(II) porphyrins like a candidate photosensitizers to bind, selective and drug delivery for metastatic melanoma
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-11-26
    Gabriela Klein Couto, Bruna Silveira Pacheco, Victoria Mascarenhas Borba, João Carlos Rodrigues Junior, Thaís Larré Oliveira, Natália Vieira Segatto, Fabiana Kommling Seixas, Thiago V. Acunha, Bernardo Iglesias, Tiago Collares

    Photodynamic therapy (PDT) is an expanding treatment modality due to its minimally invasive localized activity and few adverse effects. This therapy requires photosensitive compounds, which have high sensitivity to light exposure. Thus, in this work, the in vitro antitumor activity of meso-tetra(3- and 4-pyridyl)porphyrins (3-TPyP and 4-TPyP) in metastatic melanoma cell (WM1366 line) and non-tumoral Ovarian lineage Chinese Hamister (CHO) was evaluated using photodynamic process. Cell viability tests, molecular docking, annexin V, confocal microscopy and qRT-PCR were performed. Our results show that both porphyrins inhibited the viability of metastatic melanoma cells when exposed to light and did not alter viability in the dark. In addition, they did not demonstrate cytotoxicity in non-tumor cells. Molecular coupling demonstrated platinum porphyrin affinity for the N-terminal region of APO B-100, LDL receptor, and therefore of the cells under study. Genes such as Caspase 3 and 9, P21, Bax / BCL2, MnSod and GSH showed increased expression. For meta isomer 3-PtTPyP treatment, caspase-9 and caspase-3 expression levels showed a 4.89 and 3.23-fold increase, respectively, while for the para isomer 4-PtTPyP, this change was 3.77 and 12.16-fold, respectively. We also observed an upregulated expression of p21, a protein well-known by its action in cell cycle arrest in a p53-dependent manner. Conclusion: 3-PtTPyP and 4-PtTPyP demonstrated antitumor effect on WM1366 cells, inducing apoptosis and significant alteration of cell cytoskeleton actin. Our work shows that platinum(II) porphyrins may be promising photosensitizers for the treatment of metastatic melanoma by PDT.

    更新日期:2019-11-27
  • Trigonelline, a naturally occurring alkaloidal agent protects ultraviolet-B (UV-B) -irradiation -induced apoptotic cell death in human skin fibroblasts via attenuation of oxidative stress, restoration of cellular calcium homeostasis and prevention of endoplasmic reticulum (ER) stress
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-11-23
    Lone A. Nazir, Malik A. Tanveer, Niakoo H. Shahid, Raghu R. Sharma, A. Sheikh Tasduq

    It has been widely reported that ultraviolet-B (UV-B) radiations is the main extrinsic etiological agent that causes skin photodamage. UV-B exposure mediated photodamage (photo-aging/photo-carcinogenesis) to human skin is caused due to several physiological events at tissue, cellular and molecular levels that lead to impairment of skin function and integrity. In the present study, we investigated the protective role of Trigonelline (TG) against UV-B -induced photo-damage in Human Dermal Fibroblasts (Hs68 cells) and Balb/C mice. We exposed human skin fibroblasts and Balb/C mice to UV-B radiation and evaluated various parameters of cellular damage, including, oxidative stress, cytosolic calcium (Ca2+) levels, apoptotic and ER-stress marker proteins. We found that UV-B –irradiation -induced ROS generation lead to the depletion of endoplasmic reticulum (ER) calcium and increased the expression of ER stress protein markers (phosphorylated elf2α, CHOP, ATF4) as well as apoptotic protein markers (Bcl2, Bax and caspase-9) in a dose and time dependent manner in Hs68 cells. We then determined the effect of TG treatment on UV-B -induced cell death in Hs68 cells and observed that cells exposed to UV-B –irradiation and treated with TG had a significantly higher survival rate compared to cells exposed to UV-B –irradiation alone. TG treatment successfully reduced oxidative stress; restored Ca2+ homeostasis and re-established the ER function and prevented apoptotic cell death process. Our results suggest that TG can be used as a potential therapeutic/cosmeceutic agent in preventing skin photo-damage.

    更新日期:2019-11-26
  • Crown- and phosphoryl-containing metal phthalocyanines in solutions of poly(N-vinylpyrrolidone): Supramolecular organization, accumulation in cells, photo-induced generation of reactive oxygen species, and cytotoxicity
    J. Photochem. Photobiol. B Biol. (IF 4.067) Pub Date : 2019-11-23
    Mariya Lapshina, Aleksey Ustyugov, Vladimir Baulin, Alexei Terentiev, Aslan Tsivadze, Nataliya Goldshleger

    Nowadays, the study of well-known sensitizers for photodynamic therapy and search for new ones are intensively conducted. In the present work supramolecular organization of crown-ether and phosphoryl-containing phthalocyanines ({Mgcr8Pc, I, and М[R4Pc] (M = Zn2+, R = -OPhP(O)(OH)(OC5H11), II; M = 2H+, R = -OPhP(O)(OH)(OC5H11), III; M = 2H+, -OPhP(O)(OH)2), IIIa}, respectively) was studied in microheterogeneous media. The role of a metal ion of a macrocycle in monomerization of phosphoryl-containing Pc in the presence of water-soluble poly(N-vinylpyrrolidone) was revealed. Some photobiological properties of compound I as possible photosensitizer with respect to human adenocarcinoma cells, HeLa, were analyzed. So, the light and dark cytotoxicity of I (IC50 dose) was 1.83 μМ and higher than 25 μМ, respectively. The reactive oxygen species (ROS) formation studied with use of fluorescent ROS detector DCFH2 revealed the plateau on the curves of fluorescence intensity vs time after 30 min of irradiation and ROS are almost not produced after the end of irradiation. In HeLa cells, accumulation of compounds I and II as well as fluorescent DCF presence were shown by confocal microscopic images. At concentration of 5 μM, compound I easily penetrates into the cell localizing primarily in the perinuclear region, whereas compound II mainly remains in the periphery of the cells in the fluorescent-active state. The results obtained allow us to continue the study of these interesting compounds.

    更新日期:2019-11-26
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