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Microfluidics for brain endothelial cell-astrocyte interactions Organs-on-a-Chip Pub Date : 2023-12-14 Jayita Sanapathi, Pravinkumar Vipparthi, Sushmita Mishra, Alejandro Sosnik, Murali Kumarasamy
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Simple design for membrane-free microphysiological systems to model the blood-tissue barriers Organs-on-a-Chip Pub Date : 2023-12-06 By Ashlyn T. Young, Halston Deal, Gabrielle Rusch, Vladimir A. Pozdin, Ashley C. Brown, Michael Daniele
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Advancements in organs-on-chips technology for viral disease and anti-viral research Organs-on-a-Chip Pub Date : 2023-06-01 Jing Li, Haiqing Bai, Zihao Wang, Beibei Xu, Kristen N. Peters Olson, Chengyao Liu, Yinlei Su, Jiawei Hao, Jinying Shen, Xuetong Xi, Jie Zhen, Rong Yu, Rong Yu, Xin Xie, Wen-xia Tian, Fei Yu, Xiaoheng Liu, Lihe Zhang, Demin Zhou, Longlong Si
Disease models that can accurately recapitulate human pathophysiology during infection and clinical response to antiviral therapeutics are still lacking, which represents a major barrier in drug development. The emergence of human Organs-on-a-Chip that integrated microfluidics with three-dimensional (3D) cell culture, may become the potential solution for this urgent need. Human Organs-on-a-Chip aims
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Generation of cynomolgus monkey airway, liver ductal, and kidney organoids with pharmacokinetic functions Organs-on-a-Chip Pub Date : 2023-05-23 Chengfan Jiang, Dong Wang, Chao Ni, Xiao Li, Xinyue Liu, Ximin Ge, Dongmei Chen, Emmanuel Enoch Dzakah, Bing Zhao
Over the past decades, the pre-clinical evaluation of new drugs requires toxicological screening in animal models. The development of non-animal and nonclinical screening models could potentially play a role in the prediction of human pharmacokinetics of new drug candidates. In this study, we established stable organoids of the cynomolgus monkey airway, liver ductal, and kidney that could be passaged
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Advances in breast cancer organoid for individualized treatment Organs-on-a-Chip Pub Date : 2023-05-12 Xuelu Li, Dandan Zhu
Breast organoids and breast cancer organoids have been a crucial tool for mammary gland and breast cancer research. In the last several years, breast cancer organoids have shown tremendous potentials for largely mimicking the structural and functional features of the original breast cancer tissue. In this review, the cell source and strategy for generating breast cancer organoids will be introduced
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Multi-organ microphysiological system: A new paradigm for COVID-19 research Organs-on-a-Chip Pub Date : 2023-05-12 Peng Wang, Yaqing Wang, Jianhua Qin
Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2, is a systemic disease with a broad spectrum of manifestations in multiple organs. Till now, it remains unclear whether these multi-organ dysfunctions arise from direct viral infection, or indirect injuries. There is an urgent need to evaluate the impacts of SARS-CoV-2 infection on human bodies and explore the pathogenesis of extrapulmonary organ
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Blood–brain barrier microfluidic chips and their applications Organs-on-a-Chip Pub Date : 2023-05-13 Mengmeng Li, Mingyang Zhu, Ruolan Huang, Kun Wang, Zhilong Zeng, Lu Xiao, Yi Lin, Dan Liu
As a natural dynamic barrier separating blood from brain parenchyma, the blood–brain barrier (BBB) is mainly composed of brain microvascular endothelial cells (BMECs), pericytes, astrocytes, and a variety of neurons. The BBB regulates the highly selective transport of various substances between the brain and blood and maintains the stability of the central nervous system (CNS). Owing to this tight
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Facile fabrication of microperforated membranes with re-useable SU-8 molds for organs-on-chips Organs-on-a-Chip Pub Date : 2023-01-20 Pim de Haan, Klaus Mathwig, Lu Yuan, Brandon W. Peterson, Elisabeth Verpoorte
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The effect of membrane properties on cell growth in an ‘Airway barrier on a chip’ Organs-on-a-Chip Pub Date : 2022-11-29 N. Karra, J. Fernandes, J. James, E.J. Swindle, H. Morgan
An important factor in determining epithelial cell barrier integrity in an in vitro system is the support membrane on which cells are cultured. In this work a custom airway barrier on chip platform was used to investigate the impact of various support membranes on bronchial epithelial cell growth and barrier formation. The membranes had different pore sizes (0.4 μm or 3 μm), densities (1 × 108, 8 × 105
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The tumour microenvironment modulates cancer cell intravasation Organs-on-a-Chip Pub Date : 2022-10-22 Ayushi Agrawal, Somayeh Shahreza, Yousef Javanmardi, Nicolas Szita, Emad Moeendarbary
Development of three dimensional (3D) in vitro models to realistically recapitulate tumor microenvironment has the potential to improve translatability of anti-cancer drugs at the preclinical stage. To capture the in vivo complexity, these in vitro models should minimally incorporate the 3D interactions between multiple cell types, cellular structures such as vasculature and extracellular matrices
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Testis-on-chip platform to study ex vivo primate spermatogenesis and endocrine dynamics Organs-on-a-Chip Pub Date : 2022-06-16 Swati Sharma, Bastien Venzac, Thomas Burgers, Stefan Schlatt, Séverine Le Gac
Here, we report a testis-on-chip platform for the ex vivo culture of seminiferous tubules isolated from human and non-human primate testis. Tissues are cultured in a dedicated chamber with continuous perfusion via a vascular-like channel. The platform is fabricated from PDMS using a 3D printed mold, after design has been optimized, e.g., for the barrier between the culture chamber and the perfusion
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Epidermal growth factor receptor mediates the basolateral uptake of phosphorothioate-modified antisense oligonucleotides in the kidney Organs-on-a-Chip Pub Date : 2022-04-20 Pedro Caetano-Pinto, Katie Haughan, Angelique Kragl, Mladen V. Tzvetkov, Katherine S. Fenner, Simone H. Stahl
Antisense oligonucleotides (ASOs) are highly biologically stable and specific therapeutic molecules that interfere with mRNA transcription and as a result effectively reduce the expression of a protein of interest. ASOs have low drug clearance and are retained in tissues. This is particularly evident in the kidneys where they accumulate in the renal proximal tubules. Receptor-mediated endocytosis (RME)
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A physiological adipose-on-chip disease model to mimic adipocyte hypertrophy and inflammation in obesity Organs-on-a-Chip Pub Date : 2022-04-18 Chak Ming Leung, Louis Jun Ye Ong, Sangho Kim, Yi-Chin Toh
The adipose tissue is a metabolically active endocrine organ with a dynamic secretome that is known to be implicated in metabolic disorders. Various studies have demonstrated detrimental downstream endocrinal effects of dysfunctional adipose tissue on other metabolic tissues, such as skeletal muscle and liver. In vitro ‘Adipose-on-Chip’ (AOC) models have been developed as an animal-alternative experimental
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Microfluidic technologies for ex vivo tissue biopsies: A review Organs-on-a-Chip Pub Date : 2022-04-09 F.C. Garcia-Garcia, P.L. Candarlioglu, J.D. Porter, D.E. Davies, E.J. Swindle, H. Morgan
Traditional in vitro models and animal models often lack the physiological complexity or the accuracy to obtain predictive responses that are clinically translatable to humans. With the advent of microphysiological systems over recent years, new models that are able to mimic human biology more closely have been developed. The culture of whole tissue samples within microfluidic devices promises to bridge
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Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device Organs-on-a-Chip Pub Date : 2022-01-26 Jennifer E. Ortiz-Cárdenas, Jonathan M. Zatorski, Abhinav Arneja, Alyssa N. Montalbine, Jennifer M. Munson, Chance John Luckey, Rebecca R. Pompano
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Perfusable cell-laden micropatterned hydrogels for delivery of spatiotemporal vascular-like cues to tissues Organs-on-a-Chip Pub Date : 2022-01-15 Walter B. Varhue, Aditya Rane, Ramon Castellanos-Sanchez, Shayn M. Peirce, George Christ, Nathan S. Swami
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Evaluation of immunotherapies improving macrophage anti-tumor response using a microfluidic model Organs-on-a-Chip Pub Date : 2022-01-25 Mariya Chernyavska, Charlotte K.J.C. Hermans, Chilam Chan, Niklas Baumann, Thies Rösner, Jeanette H.W. Leusen, Thomas Valerius, Wouter P.R. Verdurmen
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From microfluidics to microphysiological systems: Past, present, and future Organs-on-a-Chip Pub Date : 2022-01-11 María Virumbrales-Muñoz, Jose M. Ayuso
For over a decade, we have seen significant strides in the microfluidics field that have led to the concept of microphysiological systems. These systems emerged in the early 2010s as versatile in vitro platforms that allowed researchers to mimic tissue complexity in vitro. Early models focused on showing the advantages of fluid physics at the microscale and demonstrating proof-of-concept experiments
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Systems engineering of microphysiometry Organs-on-a-Chip Pub Date : 2022-01-14 Joachim Wiest
The discipline of microphysiometry emerged at the end of the 1980s and has been progressing towards today's organs on chips and microphysiological systems approaches. The presented work reviews the development of cellular model from cellular monolayers toward 3D multi-cellular tissue constructs, along with the maturation of sensor principles and technologies. A modular classification into cellular
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Organ-on-Chip Technology for Aerobic Intestinal Host – Anaerobic Microbiota Research Organs-on-a-Chip Pub Date : 2021-12-15 Elsbeth G.B.M. Bossink, Loes I. Segerink, Mathieu Odijk
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Multi-organ-on-chip approach in cancer research Organs-on-a-Chip Pub Date : 2021-12-17 Agnieszka Zuchowska, Sandra Skorupska
Cancer is the second cause of death worldwide after heart disease. Despite the still developing anticancer therapies, the main challenge in cancer research is the establishing the appropriate predictive in vitro tumor model. Standard 2D and the increasingly used 3D cultures, as well as animal models suffer from numerous morphological and physiological differences. Current in vitro models often do not
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Current developments in modelling the tumour microenvironment in vitro: Incorporation of biochemical and physical gradients Organs-on-a-Chip Pub Date : 2021-11-25 Monieb A.M. Ahmed, Anika Nagelkerke
Tumour cell proliferation, metabolism and treatment response depend on the dynamic interaction of the tumour cells with other cellular components and physicochemical gradients present in the tumour microenvironment. Traditional experimental approaches used to investigate the dynamic tumour tissue face a number of limitations, such as lack of biological relevance for the tumour microenvironment and
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Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device Organs-on-a-Chip Pub Date : 2021-11-17 Hayley Foster, Mark Wade, James England, John Greenman, Victoria Green
This report demonstrates the ability of a microfluidic device to maintain human Graves' disease tissue enabling the isolation and characterisation of Graves' disease specific exosomes. Graves' disease (n = 7) and non-Graves’ disease (Hashimoto's thyroiditis, n = 3; follicular adenoma, n = 1) human tissue was incubated in a microfluidic device for 6 days ± dexamethasone or methimazole and effluent was
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In vitro nonalcoholic fatty liver disease model with cyclo-olefin-polymer-based microphysiological systems Organs-on-a-Chip Pub Date : 2021-11-08 Xiaopeng Wen, Koki Yoshimoto, Makoto Yamanaka, Shiho Terada, Ken-ichiro Kamei
Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver conditions, and its treatment involves curing the patients without liver transplantation. Understanding the mechanism of NAFLD initiation and progression would enable the development of new diagnostic tools and drugs; however, until now, the underlying mechanisms of this condition remain largely unknown owing to the lack
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Innervated adrenomedullary microphysiological system to model nicotine and opioid exposure Organs-on-a-Chip Pub Date : 2021-10-22 Jonathan R. Soucy, Gabriel Burchett, Ryan Brady, Kyla Nichols, David T. Breault, Abigail N. Koppes, Ryan A. Koppes
Transition to extrauterine life results in a surge of catecholamines necessary for increased cardiovascular, respiratory, and metabolic activity. Mechanisms mediating adrenomedullary catecholamine release are poorly understood. Important mechanistic insight is provided by newborns delivered by cesarean section or subjected to prenatal nicotine or opioid exposure, demonstrating impaired release of adrenomedullary
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Heart/liver-on-a-chip as a model for the evaluation of cardiotoxicity induced by chemotherapies Organs-on-a-Chip Pub Date : 2021-07-28 Pooneh Soltantabar, Erika L. Calubaquib, Ebrahim Mostafavi, Atefeh Ghazavi, Mihaela C. Stefan
Drug discovery faces challenges due to the lack of proper preclinical tests, including conventional cell cultures and animal studies. Organ-on-a-chip devices can mimic the whole-body response to therapeutics by fluidically connecting microscale cell cultures and generating a realistic model of human organs of interest. Here, we describe a pumpless heart/liver-on-a-chip (HLC) using the HepG2 hepatocellular
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Rapid 3D BioPrinting of a human iPSC-derived cardiac micro-tissue for high-throughput drug testing Organs-on-a-Chip Pub Date : 2021-04-15 Kathleen L. Miller, Yi Xiang, Claire Yu, Jacob Pustelnik, Jerry Wu, Xinyue Ma, Toshikatsu Matsui, Kenichi Imahashi, Shaochen Chen
With cardiac disease a reigning problem in the world, the need for accurate and high-throughput drug testing is paramount. 3D cardiac tissues are promising models, as they can recapitulate the cell-cell, cell-matrix, and cell-tissue interactions that impact response to a drug. Using an in-house developed micro-continuous optical printing system, we created a cardiac micro-tissue in mere seconds with
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Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro Organs-on-a-Chip Pub Date : 2021-04-19 Yagmur Demircan Yalcin, Regina Luttge
3-dimensional (3D) cell cultures better mimic natural environment of cells than 2-dimensional (2D) cell cultures to obtain in vivo like inter and intracellular responses. However, third dimension brings complexity to cell culture. Therefore, high-resolution/high-content screening in 3D is one of the most important challenges with this type of cell cultures. Although optical monitoring techniques, well-established
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Passive controlled flow for Parkinson's disease neuronal cell culture in 3D microfluidic devices Organs-on-a-Chip Pub Date : 2020-08-10 Khalid I.W. Kane, Javier Jarazo, Edinson Lucumi Moreno, Ronan M.T. Fleming, Jens C. Schwamborn
Controlled flow within a lab-on-a-chip is a critical element of successfully implementing culture protocols for differentiation and maintenance of stem cell derived neurons in microfluidic devices. There have been a multitude of passive pumping technologies that have been successfully used to control the flow within a lab-on-a-chip. However, most of which were only able to generate flow for very few
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PDMS leaching and its implications for on-chip studies focusing on bone regeneration applications Organs-on-a-Chip Pub Date : 2020-04-15 Sarah-Sophia D. Carter, Abdul-Raouf Atif, Sandeep Kadekar, Ingela Lanekoff, Håkan Engqvist, Oommen P. Varghese, Maria Tenje, Gemma Mestres
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A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds Organs-on-a-Chip Pub Date : 2020-04-09 Maria Tenje, Federico Cantoni, Ana María Porras Hernández, Sean S. Searle, Sofia Johansson, Laurent Barbe, Maria Antfolk, Hannah Pohlit
This review article describes microfabrication techniques to define chemical, mechanical and structural patterns in hydrogels and how these can be used to prepare in vivo like, i.e. biomimetic, cell culture scaffolds. Hydrogels are attractive materials for 3D cell cultures as they provide ideal culture conditions and they are becoming more prominently used. Single material gels without any modifications
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Drug delivery for traditional and emerging airway models Organs-on-a-Chip Pub Date : 2019-12-01 N. Karra,E.J. Swindle,H. Morgan
Abstract Respiratory diseases such as asthma and COPD have no cures and few new treatments. These diseases have an immutable mortality rate and impact millions of individuals worldwide. Respiratory drug development is time-consuming and costly, owing to the inability of existing models to replicate the complexity of human disease (static cell cultures and animal models). The problem is intensified
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A rapidly prototyped lung-on-a-chip model using 3D-printed molds Organs-on-a-Chip Pub Date : 2019-12-01 Jesus Shrestha,Maliheh Ghadiri,Melane Shanmugavel,Sajad Razavi Bazaz,Steven Vasilescu,Lin Ding,Majid Ebrahimi Warkiani
Abstract Organ-on-a-chip is a microfluidic cell culture model that replicates key organ-specific microarchitecture and pathophysiology in vitro. The current methods to fabricate these devices rely on softlithography, which is usually tedious, laborious, and requires adroit users as well as cleanroom facilities. Recently, the use of 3D-printing technologies for the rapid fabrication of molds for polydimethylsiloxane