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LXYL-P1-2 immobilized on magnetic nanoparticles and its potential application in paclitaxel production Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-31 Sen Zou; Tian-Jiao Chen; Dan-Yang Li; Shuai Fan; Zhao-Yong Yang; Ping Zhu
Background LXYL-P1-2 is the first reported glycoside hydrolase that can catalyze the transformation of 7-β-xylosyl-10-deacetyltaxol (XDT) to 10-deacetyltaxol (DT) by removing the D-xylosyl group at C-7 position. Successfully synthesis of paclitaxel by one-pot method combining the LXYL-P1-2 and 10-deacetylbaccatin III-10-β-O-acetyltransferase (DBAT) using XDT as a precursor, making LXYL-P1-2 a highly
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Short Tandem Repeats in plants: genomic distribution and function prediction Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-30 Lin Zhu; Hui Wu; Huayang Li; Heng Tang; Li Zhang; Houjuan Xu; Fangchan Jiao; Nian Wang; Long Yang
Background Short Tandem repeats (STR) existed as popular elements in both eukaryotic and prokaryotic genomes. Results In this study, we analyzed the characteristics, distributions, motif features of STRs within whole-genomes of 140 plant species. The results showed that STRs density was negatively correlated with the genome size. Hexa-nucleotide repeat was the most abundant type of STRs. The distribution
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Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial, antiviral activity in vitro Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-30 Ruijiao Jiang; Pengfei Zhang; Xulong Wu; Yin Wang; Tayyab Rehman; Xueping Yao; Yan Luo; Zexiao Yang
Background Cecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it
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H2S is synthesized via CBS/CSE pathway and triggered by cold during Agaricus bisporus storage Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-30 Ye Liu; Yuncheng Pei; Dan Zhu; Xuying Liu; Wenxiang Li; Fansheng Cheng
Background H2S is proved to be functioning as a signaling molecule in an array of physiological processes in the plant and animal kingdom. However, the H2S synthesis pathway and the responses to cold remain unclear in postharvest mushroom. Results The biosynthesis of H2S in the Agaricus bisporus mushroom tissues exhibited an increasing tendency during postharvest storage, and was significantly triggered
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High-level soluble expression of phospholipase D from Streptomyces chromofuscus in Escherichia coli by combinatorial optimization Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-30 Rong Wu; Jun Cao; Feixiang Liu; Meng Yang; Erzheng Su
Background phospholipase D (PLD) is used as the biocatalyst for phosphatidylserine (PS) production. In general, PLD was expressed in insoluble form in E. coli. High-level soluble expression of PLD with high activity in E. coli is very important for industrial production of PLD. Results Streptomyces chromofuscus PLD coding gene was codon optimized, cloned without signal peptide and expressed in E. coli
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Metagenomics approach to identify lignocellulose-degrading enzymes in the gut microbiota of the Chinese bamboo rat cecum Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-30 Ding-Ping Bai; Xin-Yu Lin; Yu-Qiong Hu; Zhen-Zhen Chen; Lu Chen; Yi-Fan Huang; Xiao-Hong Huang; Jian Li
Background Lignocellulose is considered a renewable organic material, but the industrial production of biofuel from lignocellulose is challenging because of the lack of highly active hydrolytic enzymes. The guts of herbivores contain many symbiotic microorganisms that have evolved to hydrolyze plant lignocellulose. Chinese bamboo rats mainly consume high-fiber foods, indicating that some members of
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Development of Kompetitive Allele Specific PCR markers for identification of persimmon varieties using genotyping-by-sequencing Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-03 Kyeong Bok Ma; Sang-Jin Yang; Ye-Seul Jo; Sam Seok Kang; Moon Nam
Background Persimmon (Diospyros kaki Thunb.) is the most widely cultivated species of the genus Diospyros. In this study, genetic diversity and variations in persimmon genotypes were investigated using single nucleotide polymorphism (SNP) markers identified by genotyping-by-sequencing (GBS) analysis. Results Ninety-five persimmon accessions grown in the Pear Research Institute, National Institute Horticultural
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Enzymatic preparation of manno-oligosaccharides from locust bean gum and palm kernel cake, and investigations into its prebiotic activity Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-03 Rui Zhang; Xin-Yue Li; Xiao-Long Cen; Qi-Hui Gao; Mei Zhang; Kong-Yue Li; Qian Wu; Yue-Lin Mu; Xiang-Hua Tang; Jun-Pei Zhou; Zun-Xi Huang
Background Manno-oligosaccharides (MOS) is known as a kind of prebiotics. Mannanase plays a key role for the degradation of mannan to produce MOS. In this study, the mannanases of glycoside hydrolase (GH) families 5 Man5HJ14 and GH26 ManAJB13 were employed to prepare MOS from locust bean gum (LBG) and palm kernel cake (PKC). The prebiotic activity and utilization of MOS were assessed in vitro using
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Identification and characterization of a novel 2R,3R-Butanediol dehydrogenase from Bacillus sp. DL01 Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-03 Mostafa Elmahmoudy; Nora Elfeky; Pu Zhongji; Yue Zhang; Yongming Bao
Background 2R,3R-butanediol dehydrogenase (R-BDH) and other BDHs contribute to metabolism of 3R/3S-Acetoin (3R/3S-AC) and 2,3-butanediol (2,3-BD), which are important bulk chemicals used in different industries. R-BDH is responsible for oxidizing the hydroxyl group at their (R) configuration. Bacillus species is a promising producer of 3R/3S-AC and 2,3-BD. In this study, R-bdh gene encoding R-BDH from
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Large-scale advances in SSR markers with high-throughput sequencing in Euphorbia fischeriana Steud Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-12-03 Hui Li; Yanshi Ma; Fangyi Pei; Haiyan Zhang; Jicheng Liu; Ming Jiang
Background Euphorbia fischeriana Steud is a very important medicinal herb and has significant medical value for healing cancer, edema and tuberculosis in China. The lack of molecular markers for Euphorbia fischeriana Steud is a dominant barrier to genetic research. For the purpose of developing many simple sequence repeat (SSR) molecular markers, we completed transcriptome analysis with the Illumina
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Characterization of AhLea-3 and its enhancement of salt tolerance in transgenic peanut plants Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-11-05 Lixian Qiao; Pingping Jiang; Yanyan Tang; Leilei Pan; Hongchang Ji; Wenjie Zhou; Hong Zhu; Jiongming Sui; Defeng Jiang; Jingshan Wang
Background Late embryogenesis abundant (LEA) proteins were reported to be related to adversity stress and drought tolerance. Lea-3 from Arachis hypogaea L. (AhLea-3) was previously found to be related to salt tolerance according to the result of transcriptome profiling and digital gene expression analysis. So, AhLea-3 was cloned and the salt tolerance was validated by transgenic peanut plants. Results
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Development of a species-specific PCR assay for authentication of Agkistrodon acutus based on mitochondrial cytochrome b gene Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-11-05 Yingnuo Li; Yanshuang Wang; Mingcheng Li; Lihua Zhang; Guang Xin Yuan
Background Agkistrodon acutus, a traditional Chinese medicine, clinically used in the treatment of rheumatism, tumor, and cardiovascular and cerebrovascular diseases. Due to the unique medicinal value and the difficulty of artificial breeding of Agkistrodon acutus, the supply of Agkistrodon acutus on the market exceeds the demand, and a large number of its adulterants are found on the market. In this
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Synthesis of β(1 → 3) and β(1 → 6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophila Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-11-05 Claudia V. Yañez-Ñeco; Fadia V. Cervantes; Lorena Amaya-Delgado; Antonio O. Ballesteros; Francisco J. Plou; Javier Arrizon
Background Milk whey, a byproduct of the dairy industry has a negative environmental impact, can be used as a raw material for added-value compounds such as galactooligosaccharides (GOS) synthesis by β-galactosidases. Results B-gal42 from Pantoea anthophila strain isolated from tejuino belonging to the glycosyl hydrolase family GH42, was overexpressed in Escherichia coli and used for GOS synthesis
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Changes in freshwater sediment microbial populations during fermentation of crude glycerol Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-11-05 Matiss Paiders; Vizma Nikolajeva; Galina Makarenkova; Liana Orola; Ilze Dimanta; Janis Kleperis
Background This work studied how the exposure to an unusual substrate forced a change in microbial populations during anaerobic fermentation of crude glycerol, a by-product of biodiesel production, with freshwater sediment used as an inoculum. Results The microbial associations almost completely (99.9%) utilized the glycerol contained in crude glycerol 6 g L−1 within four days, releasing gases, organic
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Vibrio sp. ArtGut-C1, a polyhydroxybutyrate producer isolated from the gut of the aquaculture live diet Artemia (Crustacea) Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-10-30 Marco Yévenes; Mauricio Quiroz; Fumito Maruyama; Milko Jorquera; Gonzalo Gajardo
Background Vibrio species display variable and plastic fitness strategies to survive and interact with multiple hosts, including marine aquaculture species that are severely affected by pathogenic Vibrios. The culturable Vibrio sp. strain ArtGut-C1, the focus of this study, provides new evidence of such phenotypic plasticity as it accumulates polyhydroxybutyrate (PHB), a biodegradable polymer with
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An in vitro model mimicking the complement system to favor directed phagocytosis of unwanted cells Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-10-14 Ivonne M. Bartsch; Karen Perelmuter; Mariela Bollati-Fogolín; Angelo Bartsch J.; Fanny Guzmán; Sergio H. Marshall
Background Opsonization, is the molecular mechanism by which target molecules promote interactions with phagocyte cell surface receptors to remove unwanted cells by induced phagocytosis. We designed an in vitro system to demonstrate that this procedure could be driven to eliminate adipocytes, using peptides mimicking regions of the complement protein C3b to promote opsonization and enhance phagocytosis
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First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-10-14 Consuelo Borie; Cristian Bravo; Phillip Dettleff; Nicolás Galarce; Jessica Dorner; Víctor Martínez
Background Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence
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Collagen as a source of bioactive peptides: A bioinformatics approach Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-10-14 Suleivys M. Nuñez; Fanny Guzmán; Pedro Valencia; Sergio Almonacid; Constanza Cárdenas
Background Collagen is the most abundant protein in animals and can be obtained from residues of the food industry. Its hydrolysate has many desirable properties that make it suitable as an additive in foods and cosmetics, or as a component of scaffold materials to be used in biomedicine. Results We report here the characterization of type I collagen from five different sources, namely bovine, porcine
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Improving micropropagation of some grape cultivars via boron, calcium and phosphate Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-10-14 Ahmed Ali Al-Aizari; Rashid S. Al-Obeed; Mahmoud A.H. Mohamed
Background An efficient regeneration protocol is a priority for the successful application of plant biotechnology. Grape nodal explants were used to develop a micropropagation protocol for Thompson Seedless and Taify cvs. Explants were cultured on MS medium supplemented with Kinetin or benzylaminopurine (BA) and indolebutyric acid (IBA). Results For both cultivars, axillary buds were grown, only, on
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Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-18 Saumel Pérez-Rodriguez; Octavio T. Ramírez; Mauricio A. Trujillo-Roldán; Norma A. Valdez-Cruz
Background Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in the pharmaceutical industry. Because of the great importance of precipitation methods for the processing of samples in proteomics, the acetone, methanol-chloroform
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An easy and robust method for the isolation of high quality RNA from coconut tissues Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-18 Amjad Iqbal; Yaodong Yang; Yi Wu; Jing Li; Muhammad Hamayun; Anwar Hussain; Farooq Shah
Background Coconut tissues consist of a complex network of polysaccharides, proteins, polyphenols, and lipids that can bind to nucleic acids and pose difficulty in isolation. Certainly, a vigorous method is required to isolate high quality and quantity of RNA from such tissues for the purpose of downstream experiments. In this paper, we discuss a newly developed method for the Isolation of RNA from
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Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-18 Sihu Wang; Sayed Haidar Abbas Raza; Chugang Mei; Kai Zhu; Matthew Garcia; Nicola M. Schreurs; Chengcheng Liang; Xinran Yang; Linsen Zan
Background To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus × Qinchuan crossbred cattle. Results Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein–protein interaction (PPI)
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Survival of commercial probiotic strains and their effect on dark chocolate synbiotic snack with raspberry content during the storage and after simulated digestion Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-18 Judyta Cielecka-Piontek; Marcin Dziedziński; Oskar Szczepaniak; Joanna Kobus-Cisowska; Aleksandra Telichowska; Daria Szymanowska
Background A key challenge for manufacturers of pro-health food containing active probiotic microorganisms is to develop a product with attractive sensory features along with maintenance of declared number of microorganisms during storage and transfer by alimentary tract. Results The highest concentration of polyphenols was observed in snacks without an additive of probiotics as well as those with
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Protective effect of CD73 inhibitor α, β-methylene ADP against amyloid-β-induced cognitive impairment by inhibiting adenosine production in hippocampus Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-06 Wu Song; Yong Tang; Lin Wei; Chi Zhang; Danning Song; Xueting Li; Shuang Jiang
Background Alzheimer's disease (AD) is a chronic, progressive neurodegenerative disease. Recent studies have reported the close association between cognitive function in AD and purinergic receptors in the central nervous system. In the current study, we investigated the effect of CD73 inhibitor α, β-methylene ADP (APCP) on cognitive impairment of AD in mice, and to explore the potential underlying
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Genetic diversity and differentiation of nine populations of the hard clam (Meretrix petechialis) assessed by EST-derived microsatellites Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-07 Qiaoyue Xu; Junhong Zheng; Xiwu Yan; Hongtao Nie
Background Meretrix petechialis is one of the commercially important marine bivalves. In this study, we selected six highly polymorphic EST-derived microsatellite markers to assess the genetic diversity and population differentiation on nine wild populations of Meretrix petechialis. Results The number of alleles detected per locus ranged from 4 to 30 (mean NA = 27.5) with a total of 165 alleles. The
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Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-07 Feng Li; Yingjie Xie; Xiang Gao; Mingxu Shan; Changchao Sun; Yan Dong Niu; Anshan Shan
Background Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources
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Prospective production of fructose and single cell protein from date palm waste Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-18 Meilana Dharma Putra; Ahmed E. Abasaeed; Saeed M. Al-Zahrani
Background Fructose and single cell protein are important products for the food market. Abundant amounts of low-grade dates worldwide are annually wasted. In this study, highly concentrated fructose syrups and single cell protein were obtained through selective fermentation of date extracts by Saccharomyces cerevisiae. Results The effect of air flow (0.1, 0.5, 0.75, 1, 1.25 and 1.5 vvm) and pH (4.5
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Respiration in Azotobacter vinelandii and its relationship with the synthesis of biopolymers Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-04 Tania Castillo; Andrés García; Claudio Padilla-Córdova; Alvaro Díaz-Barrera; Carlos Peña
Azotobacter vinelandii is a gram-negative soil bacterium that produces two biopolymers of biotechnological interest, alginate and poly(3-hydroxybutyrate), and it has been widely studied because of its capability to fix nitrogen even in the presence of oxygen. This bacterium is characterized by its high respiration rates, which are almost 10-fold higher than those of Escherichia coli and are a disadvantage
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Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-09-03 Jiafu Huang; Axian Huang; Luanmei Lu; Weikang Jiang; Danfeng Zhang; Qici Wu; Peng Li; Xiuyan Zhong
Background There is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus licheniformis cultured in the Agaricus bisporus industrial wastewater to produce the agricultural microbial
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Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-08-05 Salihu Ibrahim, Khadijah Nabilah Mohd Zahri, Peter Convey, Khalilah Abdul Khalil, Claudio Gomez-Fuentes, Azham Zulkarnain, Siti Aisyah Alias, Gerardo González-Rocha, Siti Aqlima Ahmad
Background The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological
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The protective ability and cellular mechanism of Koelreuteria henryi Dummer flower extract against hydrogen peroxide-induced cellular oxidative damage Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-08-04 Wen-Che Tsai, Hung-Chi Chang, Hsin-Yi Yin, Meng-Chieh Huang, Dinesh Chandra Agrawal, Hsiao-Wei Wen
Background Koelreuteria henryi Dummer is an indigenous plant in Taiwan. The species has been used in traditional folk medicine for the promotion of liver functions and for treating malaria and urethritis. The present study investigated the antioxidant activity of the flower extract of Koelreuteria henryi Dummer. The extraction conditions were optimized by the contents of total phenolic acids and total
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Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-21 Noroza Umer, Rubab Zahra Naqvi, Imran Rauf, Naveed Anjum, Patricia R. Keen, Joyce Van Eck, Georg Jander, Muhammad Asif
Background Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plant-derived insect resistance genes is a promising approach to control aphids and other sap-sucking insect pests. While expression from the constitutive 35S promoter provides broad protection
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Simple synthesis of photoluminescent carbon dots from a marine polysaccharide found in shark cartilage Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-20 Kyung Woo Kim, Tae-Young Choi, Yong Min Kwon, Jaoon Young Hwan Kim
Background For more than a decade, water-soluble, eco-friendly, biocompatible, and low-toxicity fluorescent nanomaterials have received considerable attention for their numerous in vivo and in vitro applications in biomedical imaging, disease diagnostics, and environmental monitoring. Owing to their tunable photoluminescence properties, carbon-based luminescent nanomaterials have shown great potential
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Biosynthetic L-tert-leucine using Escherichia coli co-expressing a novel NADH-dependent leucine dehydrogenase and a formate dehydrogenase Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-08 Longxing Wang, Wenjun Zhu, Zhen Gao, Hua Zhou, Fei Cao, Min Jiang, Yan Li, Honghua Jia, Ping Wei
Background L-tert-Leucine has been widely used in pharmaceutical, chemical, and other industries as a vital chiral intermediate. Compared with chemical methods, enzymatic methods to produce L-tert-leucine have unparalleled advantages. Previously, we found a novel leucine dehydrogenase from the halophilic thermophile Laceyella sacchari (LsLeuDH) that showed good thermostability and great potential for
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Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-07 Shu Canwei, Hu Xiaoyun, Nauman Ahmed, Wang Shiqi, Zhou Erxun, Liao Meide
Background Rice sheath blight (caused by Rhizoctonia solani) and tobacco mosaic virus are very important plant diseases, causing a huge loss in global crop production. Paenibacillus kribbensis PS04 is a broad-spectrum biocontrol agent, used for controlling these diseases. Previously, extracellular polysaccharides (EPS) from P. kribbensis PS04 had been purified and their structure was inferred to be
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Biosurfactant and bioemulsifier as promising molecules produced by Mucor hiemalis isolated from Caatinga soil Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-03 Isabela Natália Silva Ferreira, Dayana Montero Rodríguez, Galba Maria Campos-Takaki, Rosileide Fontenele da Silva Andrade
Background The present study describes the production of biosurfactant (BS) and emulsifier (BE) by the filamentous fungus Mucor hiemalis UCP 0039, as well as the characterization and stability of the both biomolecules for environmental or industrial applications. Results Biosurfactants and bioemulsifiers are amphiphilic compounds and are produced as extracellular molecules. The results showed that
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Analysis of the population structure and genetic diversity of the red swamp crayfish (Procambarus clarkii) in China using SSR markers Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-07-02 Fei Liu, Yun-Kun Qu, Chao Geng, Ai-Ming Wang, Jia-Hong Zhang, Jin-Feng Li, Kai-Jian Chen, Bo Liu, Hong-Yan Tian, Wen-Ping Yang, Ye-Bing Yu
Background Procambarus clarkii produces high-quality, delicious meat that is high in protein, low in fat, and rich in calcium and phosphorus. It has become an important aquatic resource in China. Our objectives are (i) to analyze the level of genetic diversity of P. clarkii populations; (ii) to explore the genetic differentiation (Gst); and (iii) to propose appropriate strategies for the conservation
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Enzymatic synthesis of coconut oil based wax esters by immobilized lipase EQ3 and commercial lipozyme RMIM Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-20 Pakpimol Ungcharoenwiwat, Aran H-Kittikun
Background Liquid wax esters are widely used in cosmetic as well as pharmaceutical and other industries. The demand of organic and natural products is increasing nowadays. Coconut oil contains benefit fatty acids and has been mainly used for oil-based and moisturizer products. Liquid wax esters from coconut oil and unsaturated fatty alcohol can be synthesized by enzymatic reaction; and it is interesting
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Short-chain fatty acids production by Bifidobacterium species in the presence of salep Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-18 Buse Usta-Gorgun, Lutfiye Yilmaz-Ersan
Background Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Because of the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of
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Cichoric acid from extracted Echinacea purpurea induces the proliferation and apoptosis of peripheral blood mononuclear cells from yaks Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-13 Meng-jie Wang, Sayed Haidar Abbas Raza, Qiang Wu, Cai-hua Xue, Jia-hua Liu, Long-fei Zhang, Wen-ying Zhang, Ai-chao Wang, Hua Wu
Background Cichoric acid (CA) is extracted from Echinacea purpurea. It is well known and widely used for its immunological function. However, the effect of CA on peripheral blood mononuclear cells (PBMCs) from yaks is still unclear. This study investigated the potential influences of CA on the proliferation, cytokine induction, and apoptosis of PBMCs from Datong yak in vivo, and aimed to provide a
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Effects of exogenous γ-Aminobutyric acid on the regulation of respiration and protein expression in germinating seeds of mungbean (Vigna radiata) under salt conditions Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-08 Jing Ji, Shengqing Shi, Wei Chen, Tiantian Xie, Changjian Du, Jiacheng Sun, Zheng Shi, Rongfu Gao, Zeping Jiang, Wenfa Xiao
Background γ-Aminobutyric acid (GABA) bypasses the TCA cycle via GABA shunt, suggesting a relationship with respiration. However, little is known about its role in seed germination under salt conditions. Results In this study, exogenous GABA was shown to have almost no influence on mungbean seed germination, except 0.1 mM at 10 h, while it completely alleviated the inhibition of germination by salt
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Enhancement of ethanol production efficiency in repeated-batch fermentation from sweet sorghum stem juice: Effect of initial sugar, nitrogen and aeration Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-08 Benjaporn Sriputorn, Pattana Laopaiboon, Niphaphat Phukoetphim, Nilubol Polsokchuak, Khanittha Butkun, Lakkana Laopaiboon
Background Ethanol concentration (PE), ethanol productivity (QP) and sugar consumption (SC) are important values in industrial ethanol production. In this study, initial sugar and nitrogen (urea) concentrations in sweet sorghum stem juice (SSJ) were optimized for high PE (≥ 10%, v/v), QP, (≥ 2.5 g/L·h) and SC (≥ 90%) by Saccharomyces cerevisiae SSJKKU01. Then, repeated-batch fermentations under normal
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Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-06-07 Sadia Aziz, Syeda Firdous, Hifz Rahman, Shahid Iqbal Awan, Vincent Michael, Geoffrey Meru
Background Pomegranate (Punica granatum L.), one of the most important tropical fruits in Azad Jammu and Kashmir regions of Pakistan, is highly valued for its nutrition and medicinal purposes. Although pomegranate is native to this region, the genetic diversity among wild pomegranate accessions is currently unknown. Such information would be vital for germplasm conservation and breeding efforts. In
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Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-05-24 Xianjun Li, Junhuan Wang, Wei Wu, Yang Jia, Shuanghu Fan, Thet Su Hlaing, Ibatsam Khokhar, Yanchun Yan
Background Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPE-degrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. Results A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as
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Effect of dietary nutrition on tail fat deposition and evaluation of tail-related genes in fat-tailed sheep Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-05-23 Jie Zeng, Shiwei Zhou, Yuxin Yang, Junli Du, Danju Kang, Xiaolong Wang, Yulin Chen
Background The effects of dietary nutrition on tail fat deposition and the correlation between production performance and the Hh signaling pathway and OXCT1 were investigated in fat-tailed sheep. Tan sheep were fed different nutritional diets and the variances in tail length, width, thickness and tail weight as well as the mRNA expression of fat-related genes (C/EBPα, FAS, LPL, and HSL) were determined
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Biosurfactant produced by Candida utilis UFPEDA1009 with potential application in cookie formulation Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-05-15 Beatriz Galdino Ribeiro, Bruno Oliveira de Veras, Jaciana dos Santos Aguiar, Jenyffer Medeiros Campos Guerra, Leonie Asfora Sarubbo
Background Biosurfactants are biomolecules that have the potential to be applied in food formulations due to their low toxicity and ability to improve sensory parameters. Considering the ability of yeasts to produce biosurfactants with food-friendly properties, the aim of the present study was to apply a biosurfactant produced by Candida utilis in the formulation of cookies. Results The biosurfactant
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Characterization of the biosorption of fast black azo dye K salt by the bacterium Rhodopseudomonas palustris 51ATA strain Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-05-14 Ayten Öztürk, Emel Bayol, Meysun I. Abdullah
Background Removal of dyes from wastewater by microorganisms through adsorption, degradation, or accumulation has been investigated. Biological methods used for dye treatment are generally always effective and environmentally friendly. In this study, biosorption of the Fast Black K salt azo dye by the bacterium Rhodopseudomonas palustris 51ATA was studied spectrophotometrically, at various pH (2–10)
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Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-05-03 Haiying Wu, Sijie Li, Minghua Ji, Qiao Chen, Jiping Shi, Jenny M. Blamey, Junsong Sun
Background Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this study, one of key regulators that affect the cell growth and PHB content was confirmed and analyzed.
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Development and application of two novel functional molecular markers of BADH2 in rice Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-04-26 Wenbo Li, Xiuhong Zeng, Shuanglan Li, Fabo Chen, Jian Gao
Background Fragrance is one of the most important quality traits in rice, and the phenotype is attributed to the loss-of-function betaine aldehyde dehydrogenase (BADH2) gene. At least 12 allelic variations of BADH2 have been identified, and some of these have been applied to rice fragrance breeding using traditional molecular markers and Sanger sequencing techniques. However, these traditional methods
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Cardioprotective effect of taurine and β-alanine against cardiac disease in myocardial ischemia and reperfusion-induced rats Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-04-17 Xiaolu Hou, Guizhi Sun, Ling Guo, Zhaowei Gong, Ying Han, Xiuping Bai
Background The present study analyzed the synergistic protective effect of β-alanine and taurine against myocardial ischemia/reperfusion. Myocardial infarct size, lipid peroxidation, and levels of glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), catalase, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), reactive oxygen species (ROS), apoptosis, and the mRNA
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A bioinformatics approach revealed the transcription factors of Helicobacter pylori pathogenic genes and their regulatory network nodes Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-04-06 Yu Bai, Wenliang Li, Guangyu Xu, Guihua Cui
Background Helicobacter pylori is a chronic pathogenic bacteria that causes gastric mucosal damage through various host-related and pathogen-related factors. Thus, a single gene research cannot fully explain its pathogenicity. Purpose of the study: It is necessary to establish a Helicobacter pylori pathogenic gene transcription factor regulatory network (TFRN) and study its central nodes. Results The
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Toxicity evaluation of water extract of tissue-cultured Taraxacum formosanum by acute, subacute administration, and Ames test Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-04-06 Wen-Che Tsai, Hung-Chi Chang, Yi-Han Tseng, Hsin-Yi Yin, Jiunn-Wang Liao, Dinesh Chandra Agrawal, Hsiao-Wei Wen
Background Taraxacum species (commonly known as dandelion) used as herbal medicine have been reported to exhibit an antiproliferative effect on hepatoma cells and antitumor activity in non-small-cell lung cancer cells. Although several investigations have demonstrated the safety of Taraxacum officinale, the safety of tissue-cultured plants of T. formosanum has not been assessed so far. Therefore, the
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Next generation sequencing and microbiome's taxonomical characterization of frozen soil of north western Himalayas of Jammu and Kashmir, India Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-03-24 Verruchi Gupta, Inderpal Singh, Shafaq Rasool, Vijeshwar Verma
Background Traditionally, microbial genome sequencing has been restrained to the species grown in pure culture. The development of culture-independent techniques over the last decade allows scientists to sequence microbial communities directly from environmental samples. Metagenomics is the study of complex genome by the isolation of DNA of the whole community. Next generation sequencing (NGS) of metagenomic
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LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-03-15 Rong H. Yin, Yan R. Wang, Su J. Zhao, Rong L. Yin, Man Bai, Ze Y. Wang, Yu B. Zhu, Yu Y. Cong, Hai Y. Liu, Wen L. Bai
Background Long non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown. Results In the present investigation, we verified that lncRNA-599554 had significantly
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Ectopic expression of Chrysanthemum CDM19 in Arabidopsis reveals a novel function in carpel development Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-03-10 Stephen Kironji Githeng’u, Lian Ding, Kunkun Zhao, Wenqian Zhao, Sumei Chen, Jiafu Jiang, Fadi Chen
Background APETALA3 (AP3) has significant roles in petal and stamen development in accordance with the classical ABC model. Results The AP3 homolog, CDM19, from Chrysanthemum morifolium cv. Jinba was cloned and sequenced. Sequence and phylogenetic analyses revealed that CDM19 is of DEF/AP3 lineage possessing the characteristic MIKC-type II structure. Expression analysis showed that CDM19 was transcribed
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Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115 Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-02-13 Karlo Guerrero, Alejandra Arancibia, Manuel Caceres, German Aroca
Background Methanol can be effectively removed from air by biofiltration (Shareefdeen et al., 1993; Babbitt et al., 2009 [1,2]). However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms (Negruţa et al., 2010 [3]), and it can be released out of the cell constituting a secondary emission. Results The total removal of methanol was
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Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-02-04 Sajish Sagia, Anamika Sharma, Surender Singh, Shivani Chaturvedi, Pawan Kumar Singh Nain, Lata Nain
Background Oleaginous yeasts can be grown on different carbon sources, including lignocellulosic hydrolysate containing a mixture of glucose and xylose. However, not all yeast strains can utilize both the sugars for lipogenesis. Therefore, in this study, efforts were made to isolate dual sugar-utilizing oleaginous yeasts from different sources. Results A total of eleven isolates were obtained, which
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Transient expression of a green fluorescent protein in tobacco and maize chloroplast Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-02-04 Sigifredo Arévalo-Gallegos, Hugo Varela-Rodríguez, Héctor Lugo-Aguilar, Tania S. Siqueiros-Cendón, Blanca F. Iglesias-Figueroa, Edward A. Espinoza-Sánchez, Gerardo A. Aguado-Santacruz, Quintín Rascón-Cruz
Background Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant condition of the crop. Results In this study, we constructed two different vectors with homologous
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Oxidative foliar photo-necrosis produced by the bacteria Pseudomonas cedrina Electron. J. Biotechnol. (IF 2.894) Pub Date : 2020-01-28 Guillermo Mendoza, Leonardo Sánchez-Tafolla, Ángel Trigos
Background Although bioactive metabolites capable of causing oxidative photo-necrosis in plant tissues have been identified in fungi, little is known about this type of mechanism in bacteria. These metabolites act as photosensitizers that generate reactive oxygen species (ROS) capable of causing damage to cells. In addition, these metabolites can pass into an energetically excited state when they receive