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Combinatory transplantation of mesenchymal stem cells with flavonoid small molecule in acellular nerve graft promotes sciatic nerve regeneration J. Tissue Eng. (IF 5.352) Pub Date : 2020-12-29 Wen-yuan Li; Hua Jia; Zhen-Dong Wang; Feng-guo Zhai; Guang-da Sun; Duo Ma; Gui-Bo Liu; Chun-Mei Li; Ying Wang
Previous animal studies have demonstrated that the flavonoid small-molecule TrkB agonist, 7, 8-dihydroxyflavone (DHF), promotes axon regeneration in transected peripheral nerves. In the present study, we investigated the combined effects of 7, 8-DHF treatment and bone marrow-derived stem/stromal cells (BMSCs) engraftment into acellular nerve allografts (ANAs) and explore relevant mechanisms that may
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Generating intrafusal skeletal muscle fibres in vitro: Current state of the art and future challenges J. Tissue Eng. (IF 5.352) Pub Date : 2020-12-29 Philip Barrett; Tom J Quick; Vivek Mudera; Darren J Player
Intrafusal fibres are a specialised cell population in skeletal muscle, found within the muscle spindle. These fibres have a mechano-sensory capacity, forming part of the monosynaptic stretch-reflex arc, a key component responsible for proprioceptive function. Impairment of proprioception and associated dysfunction of the muscle spindle is linked with many neuromuscular diseases. Research to-date has
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Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration J. Tissue Eng. (IF 5.352) Pub Date : 2020-12-17 Dong Joon Lee; Patricia Miguez; Jane Kwon; Renie Daniel; Ricardo Padilla; Samuel Min; Rahim Zalal; Ching-Chang Ko; Hae Won Shin
Scaffolds that are used for bone repair should provide an adequate environment for biomineralization by mesenchymal stem cells (MSCs). Recently, decellularized pulp matrices (DPM) have been utilized in endodontics for their high regenerative potential. Inspired by the dystrophic calcification on the pulp matrix known as pulp stone, we developed acellular pulp bioscaffolds and examined their potential
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Extracellular vesicles derived from human dental pulp stem cells promote osteogenesis of adipose-derived stem cells via the MAPK pathway J. Tissue Eng. (IF 5.352) Pub Date : 2020-12-02 Qiaoqiao Jin; Peilun Li; Keyong Yuan; Fen Zhao; Xiaohan Zhu; Pengfei Zhang; Zhengwei Huang
Recent studies have shown that co-culture systems play an important role in bone tissue engineering. In this study, human dental pulp stem cells (hDPSCs) were co-cultured with human adipose-derived stem cells (hADSCs), and osteoblastic phenotypes were found to be enhanced in co-cultures compared with monocultures of hDPSCs or hADSCs. Furthermore, GW4869, an inhibitor of extracellular vesicle (EV) formation
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Regeneration of skeletal system with genipin crosslinked biomaterials J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-29 Zhonghan Wang; He Liu; Wenbin Luo; Tianyang Cai; Zuhao Li; Yuzhe Liu; Weinan Gao; Qian Wan; Xianggang Wang; Jincheng Wang; Yanbing Wang; Xiaoyu Yang
Natural biomaterials, such as collagen, gelatin, and chitosan, are considered as promising candidates for use in tissue regeneration treatment, given their similarity to natural tissues regarding components and structure. Nevertheless, only receiving a crosslinking process can these biomaterials exhibit sufficient strength to bear high tensile loads for use in skeletal system regeneration. Recently
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Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-27 Ziquan Wu; Zhulong Meng; Qianjin Wu; Delu Zeng; Zhengdong Guo; Jiangling Yao; Yangyang Bian; Yuntao Gu; Shaowen Cheng; Lei Peng; Yingzheng Zhao
Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide
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In vivo study of polyurethane and tannin-modified hydroxyapatite composites for calvarial regeneration J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-21 Xinggui Tian; Xiaowei Yuan; Daxiong Feng; Min Wu; Yuping Yuan; Chuying Ma; Denghui Xie; Jinshan Guo; Chao Liu; Zhihui Lu
Biomaterial mediated bone regeneration is an attractive strategy for bone defect treatment. Organic/inorganic composites have been well established as effective bone graft. Here, the bone regenerative effect of the composites made from tannic acid (TA) modified hydroxyapatite (HA) (THA) or TA & silver nanoparticles (Ag NPs) modified HA (Ag-THA) and polyurethane (PU) was evaluated on critical-sized
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Gut-on-chip: Recreating human intestine in vitro J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-18 Yunqing Xiang; Hui Wen; Yue Yu; Mingqiang Li; Xiongfei Fu; Shuqiang Huang
The human gut is important for food digestion and absorption, as well as a venue for a large number of microorganisms that coexist with the host. Although numerous in vitro models have been proposed to study intestinal pathology or interactions between intestinal microbes and host, they are far from recapitulating the real intestinal microenvironment in vivo. To assist researchers in further understanding
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Comprehensive proteomic atlas of skin biomatrix scaffolds reveals a supportive microenvironment for epidermal development J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-10 Ling Leng; Jie Ma; Xuer Sun; Baolin Guo; Fanlu Li; Wei Zhang; Mingyang Chang; Jinmei Diao; Yi Wang; Wenjuan Wang; Shuyong Wang; Yunping Zhu; Fuchu He; Lola M Reid; Yunfang Wang
Biomaterial scaffolds are increasingly being used to drive tissue regeneration. The limited success so far in human tissues rebuilding and therapy application may be due to inadequacy of the functionality biomaterial scaffold. We developed a new decellularized method to obtain complete anatomical skin biomatrix scaffold in situ with extracellular matrix (ECM) architecture preserved, in this study.
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Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential J. Tissue Eng. (IF 5.352) Pub Date : 2020-11-06 Anna Diez-Escudero; Hugo Harlin; Per Isaksson; Cecilia Persson
Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed
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3D-printable zwitterionic nano-composite hydrogel system for biomedical applications J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-29 Nathalie Sällström; Andrew Capel; Mark P Lewis; Daniel S Engstrøm; Simon Martin
Herein, the cytotoxicity of a novel zwitterionic sulfobetaine hydrogel system with a nano-clay crosslinker has been investigated. We demonstrate that careful selection of the composition of the system (monomer to Laponite content) allows the material to be formed into controlled shapes using an extrusion based additive manufacturing technique with the ability to tune the mechanical properties of the
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Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-24 Carlotta Peticone; David De Silva Thompson; Nikolay Dimov; Ben Jevans; Nick Glass; Martina Micheletti; Jonathan C Knowles; Hae-Won Kim; Justin J Cooper-White; Ivan B Wall
Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together
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Magnesium hydroxide-incorporated PLGA composite attenuates inflammation and promotes BMP2-induced bone formation in spinal fusion J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-24 Tarek M. Bedair; Chang Kyu Lee; Da-Seul Kim; Seung-Woon Baek; Hanan M. Bedair; Hari Prasad Joshi; Un Yong Choi; Keun-Hong Park; Wooram Park; InBo Han; Dong Keun Han
Spinal fusion has become a common surgical technique to join two or more vertebrae to stabilize a damaged spine; however, the rate of pseudarthrosis (failure of fusion) is still high. To minimize pseudarthrosis, bone morphogenetic protein-2 (BMP2) has been approved for use in humans. In this study, we developed a poly(lactide-co-glycolide) (PLGA) composite incorporated with magnesium hydroxide (MH)
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The combination of multi-functional ingredients-loaded hydrogels and three-dimensional printed porous titanium alloys for infective bone defect treatment J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-23 Shichong Qiao; Dongle Wu; Zuhao Li; Yu Zhu; Fei Zhan; Hongchang Lai; Yingxin Gu
Biomaterial with the dual-functions of bone regeneration and antibacterial is a novel therapy for infective bone defects. Three-dimensional (3D)-printed porous titanium (pTi) benefits bone ingrowth, but its microporous structure conducive to bacteria reproduction. Herein, a multifunctional hydrogel was prepared from dynamic supramolecular assembly of sodium tetraborate (Na2B4O7), polyvinyl alcohol
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Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-20 Karen M Marshall; Janos M Kanczler; Richard OC Oreffo
The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the
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3D brain tissue physiological model with co-cultured primary neurons and glial cells in hydrogels J. Tissue Eng. (IF 5.352) Pub Date : 2020-10-14 Ilaria Raimondi; Marta Tunesi; Gianluigi Forloni; Diego Albani; Carmen Giordano
Recently, researchers have focused on the role of gut microbiota on human health and reported the existence of a bidirectional relationship between intestinal microbiota and the brain, referred to as microbiota-gut-brain axis (MGBA). In this context, the development of an organ-on-a-chip platform recapitulating the main players of the MGBA would help in the investigations of the biochemical mechanisms
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Engineering 3D degradable, pliable scaffolds toward adipose tissue regeneration; optimized printability, simulations and surface modification J. Tissue Eng. (IF 5.352) Pub Date : 2020-09-16 Shubham Jain; Mohammed Ahmad Yassin; Tiziana Fuoco; Hailong Liu; Samih Mohamed-Ahmed; Kamal Mustafa; Anna Finne-Wistrand
We present a solution to regenerate adipose tissue using degradable, soft, pliable 3D-printed scaffolds made of a medical-grade copolymer coated with polydopamine. The problem today is that while printing, the medical grade copolyesters degrade and the scaffolds become very stiff and brittle, being not optimal for adipose tissue defects. Herein, we have used high molar mass poly(L-lactide-co-trimethylene
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In vivo combinatory gene therapy synergistically promotes cardiac function and vascular regeneration following myocardial infarction J. Tissue Eng. (IF 5.352) Pub Date : 2020-09-04 Sunghun Lee; Bong-Woo Park; Yong Jin Lee; Kiwon Ban; Hun-Jun Park
Since myocardial infarction (MI) excessively damage the myocardium and blood vessels, the therapeutic approach for treating MI hearts should simultaneously target these two major components in the heart to achieve comprehensive cardiac repair. Here, we investigated a combinatory platform of ETV2 and Gata4, Mef2c and Tbx5 (GMT) transcription factors to develop a strategy that can rejuvenate both myocardium
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A universal multi-platform 3D printed bioreactor chamber for tendon tissue engineering. J. Tissue Eng. (IF 5.352) Pub Date : 2020-09-01 Adam J Janvier,Elizabeth Canty-Laird,James R Henstock
A range of bioreactors use linear actuators to apply tensile forces in vitro, but differences in their culture environments can limit a direct comparison between studies. The widespread availability of 3D printing now provides an opportunity to develop a ‘universal’ bioreactor chamber that, with minimal exterior editing can be coupled to a wide range of commonly used linear actuator platforms, for
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Mesenchymal stem cells for cartilage regeneration. J. Tissue Eng. (IF 5.352) Pub Date : 2020-08-26 Hanxiang Le,Weiguo Xu,Xiuli Zhuang,Fei Chang,Yinan Wang,Jianxun Ding
Cartilage injuries are typically caused by trauma, chronic overload, and autoimmune diseases. Owing to the avascular structure and low metabolic activities of chondrocytes, cartilage generally does not self-repair following an injury. Currently, clinical interventions for cartilage injuries include chondrocyte implantation, microfracture, and osteochondral transplantation. However, rather than restoring
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Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate). J. Tissue Eng. (IF 5.352) Pub Date : 2020-08-23 Hye Sung Kim,Junyu Chen,Lin-Ping Wu,Jihua Wu,Hua Xiang,Kam W Leong,Jing Han
To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cast films or electrospun nanofibrous meshes. As 3HV content increases, the crystallinity
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A miniaturized hydrogel-based in vitro model for dynamic culturing of human cells overexpressing beta-amyloid precursor protein. J. Tissue Eng. (IF 5.352) Pub Date : 2020-08-18 Marta Tunesi,Luca Izzo,Ilaria Raimondi,Diego Albani,Carmen Giordano
Recent findings have highlighted an interconnection between intestinal microbiota and the brain, referred to as microbiota–gut–brain axis, and suggested that alterations in microbiota composition might affect brain functioning, also in Alzheimer’s disease. To investigate microbiota–gut–brain axis biochemical pathways, in this work we developed an innovative device to be used as modular unit in an engineered
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Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-31 Haotian Bai,Noh Kyu-Cheol,Zhonghan Wang,Yutao Cui,He Liu,Hou Liu,Yubin Feng,Yue Zhao,Quan Lin,Zuhao Li
A diabetic foot ulcer (DFUs) is a state of prolonged chronic inflammation, which can result in amputation. Different from normal skin wounds, various commercially available dressings have not sufficiently improved the healing of DFUs. In this study, a novel self-healing hydrogel was prepared by in situ crosslinking of N-carboxyethyl chitosan (N-chitosan) and adipic acid dihydrazide (ADH) with hyaluronic
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Hydrogel and neural progenitor cell delivery supports organotypic fetal spinal cord development in an ex vivo model of prenatal spina bifida repair. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-24 Juan C Biancotti,Kendal A Walker,Guihua Jiang,Julie Di Bernardo,Lonnie D Shea,Shaun M Kunisaki
Studying how the fetal spinal cord regenerates in an ex vivo model of spina bifida repair may provide insights into the development of new tissue engineering treatment strategies to better optimize neurologic function in affected patients. Here, we developed hydrogel surgical patches designed for prenatal repair of myelomeningocele defects and demonstrated viability of both human and rat neural progenitor
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Decellularised scaffolds: just a framework? Current knowledge and future directions. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-22 Elena García-Gareta,Yousef Abduldaiem,Prasad Sawadkar,Christos Kyriakidis,Ferdinand Lali,Karin Vicente Greco
The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas
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Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-21 Camila Hochman-Mendez,Dilza Balteiro Pereira de Campos,Rafael Serafim Pinto,Bernardo Jorge da Silva Mendes,Gustavo Miranda Rocha,Gustavo Monnerat,Gilberto Weissmuller,Luiz C Sampaio,Adriana Bastos Carvalho,Doris A Taylor,Antonio Carlos Campos de Carvalho
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical
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Effect of microenvironment on adhesion and differentiation of murine C3H10T1/2 cells cultured on multilayers containing collagen I and glycosaminoglycans. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-16 Mingyan Zhao,Reema Anouz,Thomas Groth
Polyelectrolyte multilayer coating is a promising tool to control cellular behavior. Murine C3H10T1/2 embryonic fibroblasts share many features with mesenchymal stem cells, which are good candidates for use in regenerative medicine. However, the interactions of C3H10T1/2 cells with polyelectrolyte multilayers have not been studied yet. Hence, the effect of molecular composition of biomimetic multilayers
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RNA interference in glial cells for nerve injury treatment. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-06 Junquan Lin,Seung Bin Jo,Tae-Hyun Kim,Hae-Won Kim,Sing Yian Chew
Drivers of RNA interference are potent for manipulating gene and protein levels, which enable the restoration of dysregulated mRNA expression that is commonly associated with injuries and diseases. This review summarizes the potential of targeting neuroglial cells, using RNA interference, to treat nerve injuries sustained in the central nervous system. In addition, the various methods of delivering
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Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury. J. Tissue Eng. (IF 5.352) Pub Date : 2020-07-02 Jon Prager,Christopher F Adams,Alexander M Delaney,Guillaume Chanoit,John F Tarlton,Liang-Fong Wong,Divya M Chari,Nicolas Granger
Safe hydrogel delivery requires stiffness-matching with host tissues to avoid iatrogenic damage and reduce inflammatory reactions. Hydrogel-encapsulated cell delivery is a promising combinatorial approach to spinal cord injury therapy, but a lack of in vivo clinical spinal cord injury stiffness measurements is a barrier to their use in clinics. We demonstrate that ultrasound elastography – a non-invasive
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3D culture technologies of cancer stem cells: promising ex vivo tumor models. J. Tissue Eng. (IF 5.352) Pub Date : 2020-06-24 Chengye Zhang,Zhaoting Yang,Da-Long Dong,Tae-Su Jang,Jonathan C Knowles,Hae-Won Kim,Guang-Zhen Jin,Yanhua Xuan
Cancer stem cells have been shown to be important in tumorigenesis processes, such as tumor growth, metastasis, and recurrence. As such, many three-dimensional models have been developed to establish an ex vivo microenvironment that cancer stem cells experience under in vivo conditions. Cancer stem cells propagating in three-dimensional culture systems show physiologically related signaling pathway
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Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect. J. Tissue Eng. (IF 5.352) Pub Date : 2020-06-10 Yanan Liu,Haifeng Wang,Huixin Dou,Bin Tian,Le Li,Luyuan Jin,Zhenting Zhang,Lei Hu
Mesenchymal stem cells sheets have been verified as a promising non-scaffold strategy for bone regeneration. Alveolar bone marrow mesenchymal stem cells, derived from neural crest, have the character of easily obtained and strong multi-differential potential. However, the bone regenerative features of alveolar bone marrow mesenchymal stem cells sheets in the craniofacial region remain unclear. The
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Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration. J. Tissue Eng. (IF 5.352) Pub Date : 2020-06-07 Rui Dong,Yun Bai,Jingjin Dai,Moyuan Deng,Chunrong Zhao,Zhansong Tian,Fanchun Zeng,Wanyuan Liang,Lanyi Liu,Shiwu Dong
Recently, extracellular matrix-based tissue-engineered bone is a promising approach to repairing bone defects, and the seed cells are mostly mesenchymal stem cells. However, bone remodelling is a complex biological process, in which osteoclasts perform bone resorption and osteoblasts dominate bone formation. The interaction and coupling of these two kinds of cells is the key to bone repair. Therefore
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Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells. J. Tissue Eng. (IF 5.352) Pub Date : 2020-06-02 Aman S Chahal,Manuel Schweikle,Aina-Mari Lian,Janne E Reseland,Håvard J Haugen,Hanna Tiainen
Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels
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Novel morphological multi-scale evaluation system for quality assessment of decellularized liver scaffolds. J. Tissue Eng. (IF 5.352) Pub Date : 2020-05-27 Vladimíra Moulisová,Miroslav Jiřík,Claudia Schindler,Lenka Červenková,Richard Pálek,Jáchym Rosendorf,Janine Arlt,Lukáš Bolek,Simona Šůsová,Sandor Nietzsche,Václav Liška,Uta Dahmen
Decellularized scaffolds can serve as an excellent three-dimensional environment for cell repopulation. They maintain tissue-specific microarchitecture of extracellular matrix proteins with important spatial cues for cell adhesion, migration, growth, and differentiation. However, criteria for quality assessment of the three-dimensional structure of decellularized scaffolds are rather fragmented, usually
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Osteoarthritis treatment with a novel nutraceutical acetylated ligstroside aglycone, a chemically modified extra-virgin olive oil polyphenol. J. Tissue Eng. (IF 5.352) Pub Date : 2020-05-27 María C de Andrés,Mia S Meiss,Marina Sánchez-Hidalgo,Alejandro González-Benjumea,Jose G Fernández-Bolaños,Catalina Alarcón-de-la-Lastra,Richard Oc Oreffo
Recent studies have shown that dietary patterns confer protection from certain chronic diseases related to oxidative stress, the immune system and chronic low-grade inflammatory diseases. The aim of this study was to evaluate the anti-inflammatory potential and the capacity to attenuate cartilage degradation using extra-virgin olive oil–derived polyphenols for the treatment of osteoarthritis. Results
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Safety and function of a new pre-vascularized bioartificial pancreas in an allogeneic rat model. J. Tissue Eng. (IF 5.352) Pub Date : 2020-05-27 Jordan Magisson,Aladin Sassi,Daela Xhema,Aram Kobalyan,Pierre Gianello,Brice Mourer,Nguyen Tran,Charles-Thibault Burcez,Richard Bou Aoun,Séverine Sigrist
Cell encapsulation could overcome limitations of free islets transplantation but is currently limited by inefficient cells immune protection and hypoxia. As a response to these challenges, we tested in vitro and in vivo the safety and efficacy of a new macroencapsulation device named MailPan®. Membranes of MailPan® device were tested in vitro in static conditions. Its bio-integration and level of oxygenation
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High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration. J. Tissue Eng. (IF 5.352) Pub Date : 2020-05-18 Tom Hodgkinson,Francis Wignall,Judith A Hoyland,Stephen M Richardson
Stem cell–based regenerative strategies are promising for intervertebral disc degeneration. Stimulation of bone-marrow- and adipose-derived multipotent stem cells with recombinant human growth differentiation factor 6 (rhGDF6) promotes anabolic nucleus pulposus like phenotypes. In comparison to mesenchymal stem cells, adipose-derived multipotent stem cells exhibit greater NP-marker gene expression
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The anti-angiogenic tyrosine kinase inhibitor Pazopanib kills cancer cells and disrupts endothelial networks in biomimetic three-dimensional renal tumouroids. J. Tissue Eng. (IF 5.352) Pub Date : 2020-05-18 Katerina Stamati,Patricia A Redondo,Agata Nyga,Joana B Neves,Maxine Gb Tran,Mark Emberton,Umber Cheema,Marilena Loizidou
Pazopanib is a tyrosine kinase inhibitor used to treat renal cell carcinoma. Few in vitro studies investigate its effects towards cancer cells or endothelial cells in the presence of cancer. We tested the effect of Pazopanib on renal cell carcinoma cells (CAKI-2,786-O) in two-dimensional and three-dimensional tumouroids made of dense extracellular matrix, treated in normoxia and hypoxia. Finally, we
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Induced pluripotent stem cell-derived enteric neural crest cells repopulate human aganglionic tissue-engineered intestine to form key components of the enteric nervous system. J. Tissue Eng. (IF 5.352) Pub Date : 2020-04-27 David F Chang,Samuel M Zuber,Elizabeth A Gilliam,Laura-Marie A Nucho,Gabriel Levin,Fengnan Wang,Anthony I Squillaro,Sha Huang,Jason R Spence,Tracy C Grikscheit
Models for enteric neuropathies, in which intestinal nerves are absent or injured, are required to evaluate possible cell therapies. However, existing options, including transgenic mice, are variable and fragile. Here immunocompromised mice were implanted with human pluripotent stem cell-derived tissue-engineered small intestine 10 weeks prior to a second survival surgery in which enteric nervous system
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Alginate-encapsulated brain-derived neurotrophic factor-overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons. J. Tissue Eng. (IF 5.352) Pub Date : 2020-04-17 Jana Schwieger,Anika Hamm,Michael M Gepp,André Schulz,Andrea Hoffmann,Thomas Lenarz,Verena Scheper
The cochlear implant outcome is possibly improved by brain-derived neurotrophic factor treatment protecting spiral ganglion neurons. Implantation of genetically modified mesenchymal stem cells may enable the required long-term brain-derived neurotrophic factor administration. Encapsulation of mesenchymal stem cells in ultra-high viscous alginate may protect the mesenchymal stem cells from the recipient's
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Macrophages promote network formation and maturation of transplanted adipose tissue-derived microvascular fragments. J. Tissue Eng. (IF 5.352) Pub Date : 2020-04-09 Thomas Später,Maximilian M Menger,Ruth M Nickels,Michael D Menger,Matthias W Laschke
Adipose tissue-derived microvascular fragments rapidly reassemble into microvascular networks within implanted scaffolds. Herein, we analyzed the contribution of macrophages to this process. C57BL/6 mice received clodronate (clo)-containing liposomes for macrophage depletion, whereas animals treated with phosphate-buffered-saline-containing liposomes served as controls. Microvascular fragments were
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Ectopic transient overexpression of OCT-4 facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells J. Tissue Eng. (IF 5.352) Pub Date : 2020-03-07 Seung Hyun L Kim; Seunghun S Lee; Inseon Kim; Janet Kwon; Song Kwon; Taegeun Bae; Junho Hur; Hwajin Lee; Nathaniel S Hwang
Bone is a highly vascularized heterogeneous tissue consisting of both osteogenic and angiogenic cells. Thus, multiple cell types are needed to achieve complete bone tissue engineering. Recent studies have discovered importance of angiogenesis in bone regeneration.1–5 Vascularization provides oxygen and nutrients, which are essential for cell growth, and studies have shown correlation between low osteogenic
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Targeting with nanoparticles for the therapeutic treatment of brain diseases J. Tissue Eng. (IF 5.352) Pub Date : 2020-03-04 Guang-Zhen Jin; Atanu Chakraborty; Jung-Hwan Lee; Jonathan C Knowles; Hae-Won Kim
Brain diseases including Alzheimer’s, Parkinson’s, Huntington’s and brain tumours are globally challenging issues. While therapeutics has been significantly developed for the treatment, recent nanotechnology emerges as a potent tool to deliver therapeutics to brain. Among others, nanoparticle (NP)-mediated drug delivery has been considered promising because the NP can be tailored to target the receptors
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Pre-screening the intrinsic angiogenic capacity of biomaterials in an optimised ex ovo chorioallantoic membrane model. J. Tissue Eng. (IF 5.352) Pub Date : 2020-02-04 Nupur Kohli,Prasad Sawadkar,Sonia Ho,Vaibhav Sharma,Martyn Snow,Sean Powell,Maria A Woodruff,Lilian Hook,Elena García-Gareta
Biomaterial development for clinical applications is currently on the rise. This necessitates adequate in vitro testing, where the structure and composition of biomaterials must be specifically tailored to withstand in situ repair and regeneration responses for a successful clinical outcome. The chorioallantoic membrane of chicken embryos has been previously used to study angiogenesis, a prerequisite
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Biomimetic gradient scaffold of collagen–hydroxyapatite for osteochondral regeneration J. Tissue Eng. (IF 5.352) Pub Date : 2020-01-31 Cristian Parisi; Luca Salvatore; Lorenzo Veschini; Maria Paola Serra; Carl Hobbs; Marta Madaghiele; Alessandro Sannino; Lucy Di Silvio
Articular cartilage has no innate ability to repair when damaged due to its avascular nature, and lacks a rich source of progenitor cells and growth factors required for regeneration.1 Although a number of therapeutic approaches have been proposed to treat osteochondral defects, none to date has been proven to ensure a sustained regeneration. Current clinical approaches have shown significant limitations
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Clinical study of octacalcium phosphate and collagen composite in oral and maxillofacial surgery. J. Tissue Eng. (IF 5.352) Pub Date : 2020-01-23 Tadashi Kawai,Shinji Kamakura,Keiko Matsui,Masayuki Fukuda,Hiroshi Takano,Mitsuyoshi Iino,Shigeo Ishikawa,Hiromasa Kawana,Tomoya Soma,Eisaku Imamura,Hideki Kizu,Aya Michibata,Izumi Asahina,Keiichiro Miura,Norifumi Nakamura,Toshiro Kibe,Osamu Suzuki,Tetsu Takahashi
Octacalcium phosphate and its collagen composite have been recognized as bone substitute materials possessing osteoconductivity and biodegradation properties. We evaluated the effectiveness of octacalcium phosphate and its collagen composite used for bone augmentation in major oral and maxillofacial surgeries in a clinical trial. Octacalcium phosphate and its collagen composite were used in cases of
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Nano-graphene oxide/polyurethane nanofibers: mechanically flexible and myogenic stimulating matrix for skeletal tissue engineering. J. Tissue Eng. (IF 5.352) Pub Date : 2020-01-23 Seung Bin Jo,Uyanga Erdenebileg,Khandmaa Dashnyam,Guang-Zhen Jin,Jae-Ryung Cha,Ahmed El-Fiqi,Jonathan C Knowles,Kapil Dev Patel,Hae-Hyoung Lee,Jung-Hwan Lee,Hae-Won Kim
For skeletal muscle engineering, scaffolds that can stimulate myogenic differentiation of cells while possessing suitable mechanical properties (e.g. flexibility) are required. In particular, the elastic property of scaffolds is of importance which helps to resist and support the dynamic conditions of muscle tissue environment. Here, we developed highly flexible nanocomposite nanofibrous scaffolds
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EphrinB2 regulates osteogenic differentiation of periodontal ligament stem cells and alveolar bone defect regeneration in beagles J. Tissue Eng. (IF 5.352) Pub Date : 2019-12-16 Penglai Wang; Wen Wang; Tengyu Geng; Yi Liu; Shaoyue Zhu; Zongxiang Liu; Changyong Yuan
In severe periodontitis, bacteria-induced inflammation causes destruction of the tooth supporting structure, which if left untreated will lead to tooth loss. Current conventional strategies aiming to eliminate pathogenic biofilms and control infection and inflammation, either nonsurgically or surgically, cannot regenerate functional periodontal tissues.1,2 Even using various regenerative procedures
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High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-29 Wei Liao,Jieqing Wang,Jiecheng Xu,Fuyu You,Mingxin Pan,Xiaoping Xu,Jun Weng,Xu Han,Shao Li,Yang Li,Kangyan Liang,Qing Peng,Yi Gao
Spheroid culture is a widely used three-dimensional culture technology that simulates the three-dimensional structure of tumors in vivo and has been considered a good model for tumor research. However, current commercialized spheroid culture tools have the shortcomings of high cost or relatively poor spheroid-forming results for some special cells. To solve such problems, we designed a 3D printed,
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Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-29 Feng Zhao,Liuhua Zhou,Jingyu Liu,Zhongle Xu,Wenwen Ping,Haiyang Li,Luwei Xu,Zheng Xu,Changcheng Zhou,Min Wang,Ruipeng Jia
The formation of an effective vascular network can promote peripheral angiogenesis, ensuring an effective supply of blood, oxygen, and nutrients to an engineered bladder, which is important for bladder tissue engineering. Stromal vascular fraction cells (SVFs) promote vascularization and improve the function of injured tissues. In this study, adipose tissue-derived SVFs were introduced as an angiogenic
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Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-14 Byeong-Ung Park,Sang Min Park,Kyoung-Pil Lee,Seong Jin Lee,Yu Eun Nam,Han Sang Park,Seongsu Eom,Jeong Ok Lim,Dong Sung Kim,Hong Kyun Kim
The endothelialization on the poly (ε-caprolactone) nanofiber has been limited due to its low hydrophilicity. The aim of this study was to immobilize collagen on an ultra-thin poly (ε-caprolactone) nanofiber membrane without altering the nanofiber structure and maintaining the endothelial cell homeostasis on it. We immobilized collagen on the poly (ε-caprolactone) nanofiber using hydrolysis by NaOH
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Biochemical and biomechanical comparisions of decellularized scaffolds derived from porcine subcutaneous and visceral adipose tissue. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-14 Maohui Lin,Jinbo Ge,Xuecen Wang,Ziqing Dong,Malcolm Xing,Feng Lu,Yunfan He
Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral adipose tissue (VDAT). The retention of key collagen in decellularized matrix was analysed to study the
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Myogenic differentiation of human amniotic mesenchymal cells and its tissue repair capacity on volumetric muscle loss. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-11 Di Zhang,Kai Yan,Jing Zhou,Tianpeng Xu,Menglei Xu,Jiayi Lin,Jiaxiang Bai,Gaoran Ge,Dan Hu,Weibing Si,Yuefeng Hao,Dechun Geng
Stem cell-based tissue engineering therapy is the most promising method for treating volumetric muscle loss. Human amniotic mesenchymal cells possess characteristics similar to those of embryonic stem cells. In this study, we verified the stem cell characteristics of human amniotic mesenchymal cells by the flow cytometry analysis, and osteogenic and adipogenic differentiation. Through induction with
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Elucidation of the time-dependent degradation process in insoluble hyaluronic acid formulations with a controlled degradation rate. J. Tissue Eng. (IF 5.352) Pub Date : 2019-11-05 Akiko Uemura,Shingo Ogawa,Yasuyuki Isono,Ryou Tanaka
Degradation rate of hyaluronic acid to prolong its stability in vivo would be beneficial. We investigated a potential solution for prolonging the stability of hyaluronic acid within the body. We focused on decreasing the swelling ratio to slow the degradation rate of hyaluronic acid by insolubilizing sodium hyaluronate without using potentially harmful substances such as crosslinkers or modifiers.
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The emerging field of pancreatic tissue engineering: A systematic review and evidence map of scaffold materials and scaffolding techniques for insulin-secreting cells. J. Tissue Eng. (IF 5.352) Pub Date : 2019-10-30 Gabriel Alexander Salg,Nathalia A Giese,Miriam Schenk,Felix J Hüttner,Klaus Felix,Pascal Probst,Markus K Diener,Thilo Hackert,Hannes Götz Kenngott
A bioartificial endocrine pancreas is proposed as a future alternative to current treatment options. Patients with insulin-secretion deficiency might benefit. This is the first systematic review that provides an overview of scaffold materials and techniques for insulin-secreting cells or cells to be differentiated into insulin-secreting cells. An electronic literature survey was conducted in PubMed/MEDLINE
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Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine. J. Tissue Eng. (IF 5.352) Pub Date : 2019-10-15 Emi Hoshikawa,Taisuke Sato,Yoshitaka Kimori,Ayako Suzuki,Kenta Haga,Hiroko Kato,Koichi Tabeta,Daisuke Nanba,Kenji Izumi
Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening
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Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue-derived microvascular fragments. J. Tissue Eng. (IF 5.352) Pub Date : 2019-09-27 Matthias W Laschke,Elena Kontaxi,Claudia Scheuer,Alexander Heß,Philipp Karschnia,Michael D Menger
Angiogenesis in adipose tissue is promoted by insulin-like growth factor 1 signaling. We analyzed whether this regulatory mechanism also improves the angiogenic activity of adipose tissue-derived microvascular fragments. Murine adipose tissue-derived microvascular fragments were cultivated for 24 h in the University of Wisconsin (UW) solution supplemented with vehicle, insulin-like growth factor 1
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Advances in nanoparticle development for improved therapeutics delivery: nanoscale topographical aspect. J. Tissue Eng. (IF 5.352) Pub Date : 2019-09-17 Rajendra K Singh,Jonathan C Knowles,Hae-Won Kim
Nanoparticle-based therapeutics delivery holds great promise for the treatment of intractable diseases. The high loading of drug molecules and their precise delivery to target sites are needed to gain optimal therapeutic functions of the nanoparticle delivery system. In this communication, we highlight, among other properties of nanoparticles (e.g. size, shape, surface chemistry, and degradation),