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Nanodrug delivery systems for regulating microglial polarization in ischemic stroke treatment: A review J. Tissue Eng. (IF 8.2) Pub Date : 2024-03-13 Shuang-Yin Lei, Yu-Qian Yang, Jia-Cheng Liu, Dian-Hui Zhang, Yang Qu, Ying-Ying Sun, Hong-Jing Zhu, Sheng-Yu Zhou, Yi Yang, Zhen-Ni Guo
The incidence of ischemic stroke (IS) is rising in tandem with the global aging population. There is an urgent need to delve deeper into the pathological mechanisms and develop new neuroprotective strategies. In the present review, we discuss the latest advancements and research on various nanodrug delivery systems (NDDSs) for targeting microglial polarization in IS treatment. Furthermore, we critically
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Enhancing pre-clinical research with simplified intestinal cell line models J. Tissue Eng. (IF 8.2) Pub Date : 2024-03-05 Christina Fey, Theresa Truschel, Kristina Nehlsen, Spyridon Damigos, Julia Horstmann, Theresia Stradal, Tobias May, Marco Metzger, Daniela Zdzieblo
Two-dimensional culture remains widely employed to determine the bioavailability of orally delivered drugs. To gain more knowledge about drug uptake mechanisms and risk assessment for the patient after oral drug admission, intestinal in vitro models demonstrating a closer similarity to the in vivo situation are needed. In particular, Caco-2 cell-based Transwell® models show advantages as they are reproducible
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Advanced lung organoids for respiratory system and pulmonary disease modeling J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-23 Hyebin Joo, Sungjin Min, Seung-Woo Cho
Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, has gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell models and animal models. Nevertheless, lung organoids
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Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-14 Shijie Fan, Yadong Tan, Xiuchen Yuan, Chun Liu, Xiaoyu Wu, Ting Dai, Su Ni, Jiafeng Wang, Yiping Weng, Hongbin Zhao
Osteogenesis is caused by multiple factors, and the inflammatory response, osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), regeneration of blood vessels, and other factors must be considered in bone tissue engineering. To effectively repair bone defect, it is important to decrease excessive inflammation, enhance the differentiation of mesenchymal stem cells into osteoblasts
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Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-14 Joydeb Majumder, Elizabeth E Torr, Elizabeth A Aisenbrey, Connie S Lebakken, Peter F Favreau, William D Richards, Yanhong Yin, Qiang Chang, William L Murphy
The tailorable properties of synthetic polyethylene glycol (PEG) hydrogels make them an attractive substrate for human organoid assembly. Here, we formed human neural organoids from iPSC-derived progenitor cells in two distinct formats: (i) cells seeded on a Matrigel surface; and (ii) cells seeded on a synthetic PEG hydrogel surface. Tissue assembly on synthetic PEG hydrogels resulted in three dimensional
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Biosubstitutes for dural closure: Unveiling research, application, and future prospects of dura mater alternatives J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-09 Dolphee Khurana, Ankitha Suresh, Raghavendra Nayak, Manjunath Shetty, Rohit Kumar Sarda, Jonathan C Knowles, Hae-Won Kim, Rajendra K Singh, Bhisham Narayan Singh
The dura mater, as the crucial outermost protective layer of the meninges, plays a vital role in safeguarding the underlying brain tissue. Neurosurgeons face significant challenges in dealing with trauma or large defects in the dura mater, as they must address the potential complications, such as wound infections, pseudomeningocele formation, cerebrospinal fluid leakage, and cerebral herniation. Therefore
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Adipose tissue derived stem cell secretome induces motor and histological gains after complete spinal cord injury in Xenopus laevis and mice J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-09 Rita C Assunção-Silva, Andreia Pinho, Jorge R Cibrão, Inês M Pereira, Susana Monteiro, Nuno A Silva, Jonas Campos, Ana L Rebelo, Gerhard Schlosser, Luisa Pinto, Abhay Pandit, António J Salgado
Mesenchymal stem cell-based therapies have been studied for spinal cord injury (SCI) treatment due to their paracrine action upon damaged tissues. MSCs neuroregenerative role may relate to the contents of their secretome in anti-inflammatory cytokines and growth-permissive factors. We propose using the secretome of MSCs isolated from the adipose tissue—adipose tissue-derived stem cells (ASCs) as a
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Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-09 Rosa González-Sastre, Raquel Coronel, Adela Bernabeu-Zornoza, Patricia Mateos-Martínez, Andreea Rosca, Victoria López-Alonso, Isabel Liste
Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This
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Therapeutic potential of luteolin-loaded poly(lactic-co-glycolic acid)/modified magnesium hydroxide microsphere in functional thermosensitive hydrogel for treating neuropathic pain J. Tissue Eng. (IF 8.2) Pub Date : 2024-02-08 So-Yeon Park, Joon Hyuk Jung, Da-Seul Kim, Jun-Kyu Lee, Byeong Gwan Song, Hae Eun Shin, Ji-Won Jung, Seung-Woon Baek, Seungkwon You, Inbo Han, Dong Keun Han
Neuropathic pain (NP) is a debilitating condition stemming from damage to the somatosensory system frequently caused by nerve injuries or lesions. While existing treatments are widely employed, they often lead to side effects and lack specificity. This study aimed to alleviate NP by developing an innovative sustained-release thermosensitive hydrogel system. The system incorporates hyaluronic acid (HA)/Pluronic
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Recent advances in tissue repair of the blood-brain barrier after stroke J. Tissue Eng. (IF 8.2) Pub Date : 2024-01-31 Liujie Qi, Fei Wang, Xiaojing Sun, Hang Li, Kun Zhang, Jingan Li
The selective permeability of the blood-brain barrier (BBB) enables the necessary exchange of substances between the brain parenchyma and circulating blood and is important for the normal functioning of the central nervous system. Ischemic stroke inflicts damage upon the BBB, triggering adverse stroke outcomes such as cerebral edema, hemorrhagic transformation, and aggravated neuroinflammation. Therefore
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Strain-induced bands of Büngner formation promotes axon growth in 3D tissue-engineered constructs J. Tissue Eng. (IF 8.2) Pub Date : 2024-01-19 Carina Hromada, Dorota Szwarc-Hofbauer, Mai Quyen Nguyen, Janine Tomasch, Michaela Purtscher, David Hercher, Andreas Herbert Teuschl-Woller
Treatment of peripheral nerve lesions remains a major challenge due to poor functional recovery; hence, ongoing research efforts strive to enhance peripheral nerve repair. In this study, we aimed to establish three-dimensional tissue-engineered bands of Büngner constructs by subjecting Schwann cells (SCs) embedded in fibrin hydrogels to mechanical stimulation. We show for the first time that the application
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Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury J. Tissue Eng. (IF 8.2) Pub Date : 2024-01-10 Dan Zhu, Tie Peng, Zhenwang Zhang, Shuang Guo, Ying Su, Kangwei Zhang, Jiawei Wang, Chao Liu
Spinal cord injury (SCI) is a significant cause of disability worldwide, with limited treatment options. This study investigated the potential of bone marrow-derived mesenchymal stem cells (BMSCs) modified with XIST lentiviral vector to modulate macrophage polarization and affect neural stem cell (NSC) microenvironment reconstruction following SCI. Bioinformatics analysis revealed that MID1 might be
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Advancements in cell-based therapies for the treatment of pressure injuries: A systematic review of interventional studies. J. Tissue Eng. (IF 8.2) Pub Date : 2023-11-20 Alianda Camesi,Reto Wettstein,Ezra Valido,Nicole Nyfeler,Stevan Stojic,Marija Glisic,Jivko Stoyanov,Alessandro Bertolo
The high recurrence and complications associated with severe pressure injuries (PI) necessitate the exploration of advanced treatments, such as cell-based therapies, to facilitate wound healing. Such techniques harness the ability of different cell types to promote angiogenesis, re-epithelialization of the skin, and tissue regeneration. This systematic review explores the efficacy of cell-based therapies
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A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapy. J. Tissue Eng. (IF 8.2) Pub Date : 2023-11-17 Michael J Buckenmeyer,Meena Sukhwani,Aimon Iftikhar,Alexis L Nolfi,Ziyu Xian,Srujan Dadi,Zachary W Case,Sarah R Steimer,Antonio D'Amore,Kyle E Orwig,Bryan N Brown
Female cancer patients who have undergone chemotherapy have an elevated risk of developing ovarian dysfunction and failure. Experimental approaches to treat iatrogenic infertility are evolving rapidly; however, challenges and risks remain that hinder clinical translation. Biomaterials have improved in vitro follicle maturation and in vivo transplantation in mice, but there has only been marginal success
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An organotypic oral mucosal infection model to study host-pathogen interactions. J. Tissue Eng. (IF 8.2) Pub Date : 2023-10-21 Samantha J Gould,Andrew D Foey,Vehid M Salih
Early in vitro oral mucosal infection models (OMMs) failed to consider the suitability of the model environment to represent the host immune response. Denture stomatitis (DS) is mediated by Candida albicans, but the role of Staphylococcus aureus remains uncertain. A collagen hydrogel-based OMM containing HaCaT and HGF cell types was developed, characterised and employed to study of tissue invasion
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Engineered heart tissue maturation inhibits cardiomyocyte proliferative response to cryoinjury. J. Tissue Eng. (IF 8.2) Pub Date : 2023-10-11 Giulio Ciucci,Karim Rahhali,Giovanni Cimmino,Francesco Natale,Paolo Golino,Gianfranco Sinagra,Chiara Collesi,Francesco S Loffredo
The cellular and molecular mechanisms that are responsible for the poor regenerative capacity of the adult heart after myocardial infarction (MI) are still unclear and their understanding is crucial to develop novel regenerative therapies. Considering the lack of reliable in vitro tissue-like models to evaluate the molecular mechanisms of cardiac regeneration, we used cryoinjury on rat Engineered Heart
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Reconstructing vascular networks promotes the repair of skeletal muscle following volumetric muscle loss by pre-vascularized tissue constructs. J. Tissue Eng. (IF 8.2) Pub Date : 2023-09-21 Chih-Long Chen,Shih-Yen Wei,Wei-Lin Chen,Ting-Lun Hsu,Ying-Chieh Chen
Current treatment for complex and large-scale volumetric muscle loss (VML) injuries remains a limited success and have substantial disadvantages, due to the irreversible loss of muscle mass, slow muscle regeneration, and rapid formation of non-functional fibrosis scars. These VML injuries are accompanied by denervation and the destruction of native vasculature which increases difficulties in the functional
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Establishing an hTERT-driven immortalized umbilical cord-derived mesenchymal stem cell line and its therapeutic application in mice with liver failure. J. Tissue Eng. (IF 8.2) Pub Date : 2023-09-19 Qi Chen,Meixian Jin,Simin Wang,Kexin Wang,Liqin Chen,Xiaojuan Zhu,Ying Zhang,Yi Wang,Yang Li,Shao Li,Youmin Zeng,Lei Feng,Wanren Yang,Yi Gao,Shuqin Zhou,Qing Peng
Acute liver failure (ALF) is characterized by rapid liver cell destruction. It is a multi-etiological and fulminant complication with a clinical mortality of over 80%. Therapy using mesenchymal stem cells (MSCs) or MSCs-derived exosomes can alleviate acute liver injury, which has been demonstrated in animal experiments and clinical application. However, similar to other stem cells, different cell sources
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3D bioprinting of corneal models: A review of the current state and future outlook. J. Tissue Eng. (IF 8.2) Pub Date : 2023-09-15 Leon Balters,Stephan Reichl
The cornea is the outermost layer of the eye and serves to protect the eye and enable vision by refracting light. The need for cornea organ donors remains high, and the demand for an artificial alternative continues to grow. 3D bioprinting is a promising new method to create artificial organs and tissues. 3D bioprinting offers the precise spatial arrangement of biomaterials and cells to create 3D constructs
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Tendon tissue engineering: An overview of biologics to promote tendon healing and repair. J. Tissue Eng. (IF 8.2) Pub Date : 2023-09-13 Vera Citro,Marta Clerici,Aldo R Boccaccini,Giovanna Della Porta,Nicola Maffulli,Nicholas R Forsyth
Tendons are dense connective tissues with a hierarchical polarized structure that respond to and adapt to the transmission of muscle contraction forces to the skeleton, enabling motion and maintaining posture. Tendon injuries, also known as tendinopathies, are becoming more common as populations age and participation in sports/leisure activities increases. The tendon has a poor ability to self-heal
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Continuous nutrient supply culture strategy controls multivesicular endosomes pathway and anti-photo-aging miRNA cargo loading of extracellular vesicles. J. Tissue Eng. (IF 8.2) Pub Date : 2023-09-04 Lihao Chen,Weihan Xie,Keke Wu,Yuan Meng,Yijun He,Jiawei Cai,Yuan Jiang,Qi Zhao,Yixi Yang,Minru Zhang,Manping Lu,Shaozhang Lin,Lin Liang,Zhiyong Zhang
Extracellular vesicle (EV) therapy recently had shown significant efficacy in various diseases. Serum starvation culture (SC) is one of the most widely used methods for collecting EVs. However, SC may cause inadvertent effects and eventually dampen the therapeutic potential of EVs. Therefore, we developed a novel method for EV collection, continuous nutrient supply culture (CC), which can provide an
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In vivo vascularized scaffold with different shear-exposed models for lymphatic tissue regeneration. J. Tissue Eng. (IF 8.2) Pub Date : 2023-08-31 Hui-Yi Hsiao,Gina Alicia Mackert,Yung-Chun Chang,Jia-Wei Liu,Frank Chun-Shin Chang,Jung-Ju Huang
Current clinical treatments on lymphedema provide promising results, but also result in donor site morbidities. The establishment of a microenvironment optimized for lymphangiogenesis can be an alternative way to enhance lymphatic tissue formation. Hemodynamic flow stimuli have been confirmed to have an influential effect on angiogenesis in tissue engineering, but not on lymphatic vessel formation
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Mechanically activated mesenchymal-derived bone cells drive vessel formation via an extracellular vesicle mediated mechanism. J. Tissue Eng. (IF 8.2) Pub Date : 2023-08-29 N Shen,M Maggio,I Woods,M C Lowry,R Almasri,C Gorgun,K F Eichholz,E Stavenschi,K Hokamp,F M Roche,L O'Driscoll,D A Hoey
Blood vessel formation is an important initial step for bone formation during development as well as during remodelling and repair in the adult skeleton. This results in a heavily vascularized tissue where endothelial cells and skeletal cells are constantly in crosstalk to facilitate homeostasis, a process that is mediated by numerous environmental signals, including mechanical loading. Breakdown in
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Promoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel. J. Tissue Eng. (IF 8.2) Pub Date : 2023-08-16 Jae Wan Suh,Kyoung-Mi Lee,Eun Ae Ko,Dong Suk Yoon,Kwang Hwan Park,Hyun Sil Kim,Jong In Yook,Nam Hee Kim,Jin Woo Lee
Decreased angiogenesis contributes to delayed wound healing in diabetic patients. Recombinant human bone morphogenetic protein-2 (rhBMP2) has also been demonstrated to promote angiogenesis. However, the short half-lives of soluble growth factors, including rhBMP2, limit their use in wound-healing applications. To address this limitation, we propose a novel delivery model using a protein transduction
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Technological advances in fibrin for tissue engineering. J. Tissue Eng. (IF 8.2) Pub Date : 2023-08-14 Raúl Sanz-Horta,Ana Matesanz,Alberto Gallardo,Helmut Reinecke,José Luis Jorcano,Pablo Acedo,Diego Velasco,Carlos Elvira
Fibrin is a promising natural polymer that is widely used for diverse applications, such as hemostatic glue, carrier for drug and cell delivery, and matrix for tissue engineering. Despite the significant advances in the use of fibrin for bioengineering and biomedical applications, some of its characteristics must be improved for suitability for general use. For example, fibrin hydrogels tend to shrink
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Fibrous hydrogels by electrospinning: Novel platforms for biomedical applications. J. Tissue Eng. (IF 8.2) Pub Date : 2023-08-12 Ji Woo Lee,Kwang Hoon Song
Hydrogels, hydrophilic and biocompatible polymeric networks, have been used for numerous biomedical applications because they have exhibited abilities to mimic features of extracellular matrix (ECM). In particular, the hydrogels engineered with electrospinning techniques have shown great performances in biomedical applications. Electrospinning techniques are to generate polymeric micro/nanofibers that
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New insights into balancing wound healing and scarless skin repair. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-27 Shengxi Zhou,Mengbo Xie,Jingjing Su,Bingjie Cai,Jingan Li,Kun Zhang
Scars caused by skin injuries after burns, wounds, abrasions and operations have serious physical and psychological effects on patients. In recent years, the research of scar free wound repair has been greatly expanded. However, understanding the complex mechanisms of wound healing, in which various cells, cytokines and mechanical force interact, is critical to developing a treatment that can achieve
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Augmented effect of fibroblast growth factor 18 in bone morphogenetic protein 2-induced calvarial bone healing by activation of CCL2/CCR2 axis on M2 macrophage polarization. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-26 Worachat Namangkalakul,Shigenori Nagai,Chengxue Jin,Ken-Ichi Nakahama,Yuki Yoshimoto,Satoshi Ueha,Kazunari Akiyoshi,Kouji Matsushima,Tomoki Nakashima,Masaki Takechi,Sachiko Iseki
Fibroblast growth factor (FGF) signaling plays essential roles in various biological events. FGF18 is one of the ligands to be associated with osteogenesis, chondrogenesis and bone healing. The mouse critical-sized calvarial defect healing induced by the bone morphogenetic protein 2 (BMP2)-hydrogel is stabilized when FGF18 is added. Here, we aimed to investigate the role of FGF18 in the calvarial bone
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Method for manufacture and cryopreservation of cartilage microtissues. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-26 Md Shafiullah Shajib,Kathryn Futrega,Rose Ann G Franco,Eamonn McKenna,Bianca Guillesser,Travis J Klein,Ross W Crawford,Michael R Doran
The financial viability of a cell and tissue-engineered therapy may depend on the compatibility of the therapy with mass production and cryopreservation. Herein, we developed a method for the mass production and cryopreservation of 3D cartilage microtissues. Cartilage microtissues were assembled from either 5000 human bone marrow-derived stromal cells (BMSC) or 5000 human articular chondrocytes (ACh)
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Tissue engineering in growth plate cartilage regeneration: Mechanisms to therapeutic strategies. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-19 Ruoyi Guo,Hanjie Zhuang,Xiuning Chen,Yulong Ben,Minjie Fan,Yiwei Wang,Pengfei Zheng
The repair of growth plate injuries is a highly complex process that involves precise spatiotemporal regulation of multiple cell types. While significant progress has been made in understanding the pathological mechanisms underlying growth plate injuries, effectively regulating this process to regenerate the injured growth plate cartilage remains a challenge. Tissue engineering technology has emerged
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3D Bioprinting tissue analogs: Current development and translational implications. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-13 Suihong Liu,Lijia Cheng,Yakui Liu,Haiguang Zhang,Yongteng Song,Jeong-Hui Park,Khandmaa Dashnyam,Jung-Hwan Lee,Fouad Al-Hakim Khalak,Oliver Riester,Zheng Shi,Serge Ostrovidov,Hirokazu Kaji,Hans-Peter Deigner,José Luis Pedraz,Jonathan C Knowles,Qingxi Hu,Hae-Won Kim,Murugan Ramalingam
Three-dimensional (3D) bioprinting is a promising and rapidly evolving technology in the field of additive manufacturing. It enables the fabrication of living cellular constructs with complex architectures that are suitable for various biomedical applications, such as tissue engineering, disease modeling, drug screening, and precision regenerative medicine. The ultimate goal of bioprinting is to produce
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Extracellular matrix-derived peptide stimulates the generation of endocrine progenitors and islet organoids from iPSCs. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-08 Emma S Heaton,Ming Hu,Tianzheng Liu,Huang Hui,Yinfei Tan,Kaiming Ye,Sha Jin
Induced pluripotent stem cells (iPSCs) have enormous potential in producing human tissues endlessly. We previously reported that type V collagen (COL5), a pancreatic extracellular matrix protein, promotes islet development and maturation from iPSCs. In this study, we identified a bioactive peptide domain of COL5, WWASKS, through bioinformatic analysis of decellularized pancreatic ECM (dpECM)-derived
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Disc regeneration by injectable fucoidan-methacrylated dextran hydrogels through mechanical transduction and macrophage immunomodulation. J. Tissue Eng. (IF 8.2) Pub Date : 2023-07-03 Weifeng Li,Pinghui Zhou,Bomin Yan,Meiyao Qi,Yedan Chen,Lijun Shang,Jianzhong Guan,Li Zhang,Yingji Mao
Modulating a favorable inflammatory microenvironment that facilitates the recovery of degenerated discs is a key strategy in the treatment of intervertebral disc (IVD) degeneration (IDD). More interestingly, well-mechanized tissue-engineered scaffolds have been proven in recent years to be capable of sensing mechanical transduction to enhance the proliferation and activation of nucleus pulposus cells
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Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9. J. Tissue Eng. (IF 8.2) Pub Date : 2023-06-29 Tao Jiang,Shenghong Miao,Jingjie Shen,Wenjing Song,Shenglong Tan,Dandan Ma
The odontogenic differentiation of dental pulp stem cells (DPSCs), which is vital for tooth regeneration, was regulated by various functional molecules. In recent years, a growing body of research has shown that miRNAs play a crucial role in the odontogenic differentiation of human dental pulp stem cells (hDPSCs). However, the mechanisms by which miRNAs regulated odontogenic differentiation of hDPSCs
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SP7 gene silencing dampens bone marrow stromal cell hypertrophy, but it also dampens chondrogenesis. J. Tissue Eng. (IF 8.2) Pub Date : 2023-06-21 Rose Ann G Franco,Eamonn McKenna,Pamela G Robey,Ross W Crawford,Michael R Doran,Kathryn Futrega
For bone marrow stromal cells (BMSC) to be useful in cartilage repair their propensity for hypertrophic differentiation must be overcome. A single day of TGF-β1 stimulation activates intrinsic signaling cascades in BMSCs which subsequently drives both chondrogenic and hypertrophic differentiation. TGF-β1 stimulation upregulates SP7, a transcription factor known to contribute to hypertrophic differentiation
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Epigenetics: Novel crucial approach for osteogenesis of mesenchymal stem cells. J. Tissue Eng. (IF 8.2) Pub Date : 2023-06-16 Zhaohua Wang,Si Wen,Meiqi Zhong,Ziming Yang,Wei Xiong,Kuo Zhang,Shude Yang,Huizheng Li,Shu Guo
Bone has a robust regenerative potential, but its capacity to repair critical-sized bone defects is limited. In recent years, stem cells have attracted significant interest for their potential in tissue engineering. Applying mesenchymal stem cells (MSCs) for enhancing bone regeneration is a promising therapeutic strategy. However, maintaining optimal cell efficacy or viability of MSCs is limited by
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The rise of mechanical metamaterials: Auxetic constructs for skin wound healing. J. Tissue Eng. (IF 8.2) Pub Date : 2023-06-13 Óscar Lecina-Tejero,María Ángeles Pérez,Elena García-Gareta,Carlos Borau
Auxetic materials are known for their unique ability to expand/contract in multiple directions when stretched/compressed. In other words, they exhibit a negative Poisson's ratio, which is usually positive for most of materials. This behavior appears in some biological tissues such as human skin, where it promotes wound healing by providing an enhanced mechanical support and facilitating cell migration
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Modified black phosphorus quantum dots promotes spinal cord injury repair by targeting the AKT signaling pathway. J. Tissue Eng. (IF 8.2) Pub Date : 2023-06-12 Dong-Mei Xie,Chuanwei Sun,Qingqiang Tu,Suyi Li,Yu Zhang,Xifan Mei,Yuanlong Li
Spinal cord injury (SCI) is a serious refractory disease of the central nervous system (CNS), which mostly caused by high-energy trauma. Existing interventions such as hormone shock and surgery are insufficient options, which relate to the secondary inflammation and neuronal dysfunction. Hydrogel with neuron-protective behaviors attracts tremendous attention, and black phosphorus quantum dots (BPQDs)
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Comparison of extracellular vesicle isolation processes for therapeutic applications. J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-23 Soraya Williams,Maria Fernandez-Rhodes,Alice Law,Ben Peacock,Mark P Lewis,Owen G Davies
While extracellular vesicles (EVs) continue to gain interest for therapeutic applications, their clinical translation is limited by a lack of optimal isolation methods. We sought to determine how universally applied isolation methods impact EV purity and yield. EVs were isolated by ultracentrifugation (UC), polyethylene glycol precipitation, Total Exosome Isolation Reagent, an aqueous two-phase system
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Significance of mechanical loading in bone fracture healing, bone regeneration, and vascularization. J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-22 Qianli Ma,Zahra Miri,Håvard Jostein Haugen,Amirhossein Moghanian,Dagnjia Loca
In 1892, J.L. Wolff proposed that bone could respond to mechanical and biophysical stimuli as a dynamic organ. This theory presents a unique opportunity for investigations on bone and its potential to aid in tissue repair. Routine activities such as exercise or machinery application can exert mechanical loads on bone. Previous research has demonstrated that mechanical loading can affect the differentiation
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Osteoarthritis models: From animals to tissue engineering. J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-18 Hongyuan Dou,Shuhan Wang,Jiawei Hu,Jian Song,Chao Zhang,Jiali Wang,Lin Xiao
Osteoarthritis (OA) is a chronic degenerative osteoarthropathy. Although it has been revealed that a variety of factors can cause or aggravate the symptoms of OA, the pathogenic mechanisms of OA remain unknown. Reliable OA models that accurately reflect human OA disease are crucial for studies on the pathogenic mechanism of OA and therapeutic drug evaluation. This review first demonstrated the importance
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Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-16 Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur, Patrick S Stumpf, Richard OC Oreffo
There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to ...
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Non-hypertrophic chondrogenesis of mesenchymal stem cells through mechano-hypoxia programing J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-16 David Xinzheyang Li, Zhiyao Ma, Alexander RA Szojka, Xiaoyi Lan, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B Adesida
Cartilage tissue engineering aims to generate functional replacements to treat cartilage defects from damage and osteoarthritis. Human bone marrow-derived mesenchymal stem cells (hBM-MSC) are a pro...
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Multifunctional 3D-printed bioceramic scaffolds: Recent strategies for osteosarcoma treatment J. Tissue Eng. (IF 8.2) Pub Date : 2023-05-15 Xingran Liu, Yihao Liu, Lei Qiang, Ya Ren, Yixuan Lin, Han Li, Qiuhan Chen, Shuxin Gao, Xue Yang, Changru Zhang, Minjie Fan, Pengfei Zheng, Shuai Li, Jinwu Wang
Osteosarcoma is the most prevalent bone malignant tumor in children and teenagers. The bone defect, recurrence, and metastasis after surgery severely affect the life quality of patients. Clinically...
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COVID crisis, austerity and the ‘Left Behind’ city: Exploring poverty and destitution in Stoke-on-Trent J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 David Etherington, Martin Jones, Luke Telford
Whilst the COVID-19 pandemic and ‘cost of living’ crisis revealed and intensified the United Kingdom’s (UK) socio-spatial inequalities, these crises did not emerge into a vacuum. Long-term trends o...
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A silica/epoxy resin nanocomposite exhibiting high thermal stability and low thermal expansion based on the uniform dispersion of hydrophilic colloidal silica nanospheres J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 Mitsuru Tanahashi, Kazuma Hirota
The present study fabricated high-performance silica/epoxy resin nanocomposites having a low coefficient of linear thermal expansion (CTE) and a high glass transition temperature (Tg). This was acc...
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Book Covers and State Translation Programs: A Multimodal Approach to Book Covers in the Panda Books Series J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 Binji Zao, Huiyu Zhang, Quangong Feng
In the era of globalization, institutional translation has become increasingly important. The Panda Books Series (PBS) is an exemplar of institutional translation that translates and disseminates C...
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Partners or Providers? An Analysis of Nonprofit Federal Contractor Performance J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 Benjamin M. Brunjes
As competition over grant funding increases, nonprofit organizations (NPOs) are increasingly likely to enter into contracts with government agencies to ensure funding stability and accomplish organ...
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The power of history: How a victimization narrative shapes national identity and public opinion in China J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 Yiqing Xu, Jiannan Zhao
We study the effect of a victimization narrative on national identity and public opinion in China experimentally. Previous research has suggested that governments can shape public opinion by guidin...
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Experimentally Disentangling Donors’ Perceptions of Government-Supported Nonprofits: Cost-Efficiency, Program Impact, and Shared Services J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 ChiaKo Hung, Yuan Tian, Youlang Zhang
This study advances understanding of the relationship between government support and private donations, by further investigating the mechanisms underlying that relationship and by examining a nonmo...
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Rethinking the Role of Volunteering in the Labor Market Inclusion of Migrants J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-26 Ilona Bontenbal, Francesca Calo, Tom Montgomery, Simone Baglioni
In this article, we critically investigate the role that volunteering can have in the labor market inclusion of migrants. We consider how volunteering can both enhance and hinder inclusion through ...
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Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-21 Qiwei Li, Chunyan Wang, Xiaoran Li, Jing Zhang, Zilin Zhang, Keyu Yang, Jun Ouyang, Shaohui Zha, Lifeng Sha, Jianjun Ge, Zaozao Chen, Zhongze Gu
In vitro skin models are rapidly developing and have been widely used in various fields as an alternative to traditional animal experiments. However, most traditional static skin models are constru...
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Application of nanomaterials in the treatment of intracerebral hemorrhage J. Tissue Eng. (IF 8.2) Pub Date : 2023-04-03 Xiangyu Zhang, Suliman Khan, Ruixue Wei, Yan Zhang, Yang Liu, Voon Wee Yong, Mengzhou Xue
Intracerebral hemorrhage (ICH) is a non-traumatic hemorrhage caused by the rupture of blood vessels in the brain parenchyma, with an acute mortality rate of 30%‒40%. Currently, available treatment ...
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3-D tissue-engineered epidermis against human primary keratinocytes apoptosis via relieving mitochondrial oxidative stress in wound healing J. Tissue Eng. (IF 8.2) Pub Date : 2023-03-31 Shan He, Han Wu, Junqun Huang, Qingyan Li, Zijie Huang, Huangding Wen, Zhiqing Li
The tissue-engineered epidermal (TEE), composed of biocompatible vectors and autogenous functional cells, is a novel strategy to solve the problem of shortage of donor skin sources. The human prima...
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Regeneration of articular cartilage defects: Therapeutic strategies and perspectives J. Tissue Eng. (IF 8.2) Pub Date : 2023-03-31 Xueqiang Guo, Lingling Xi, Mengyuan Yu, Zhenlin Fan, Weiyun Wang, Andong Ju, Zhuo Liang, Guangdong Zhou, Wenjie Ren
Articular cartilage (AC), a bone-to-bone protective device made of up to 80% water and populated by only one cell type (i.e. chondrocyte), has limited capacity for regeneration and self-repair afte...
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Repairing a critical cranial defect using WISP1-pretreated chondrocyte scaffolds J. Tissue Eng. (IF 8.2) Pub Date : 2023-03-18 Idan Carmon, Shira Kalmus, Anna Zobrab, Michael Alterman, Raphaelle Emram, May Gussarsky, Leonid Kandel, Eli Reich, Nardi Casap, Mona Dvir-Ginzberg
In cranial flat bone fractures, spontaneous bone repair will occur only when the fracture ends are in close contact. However, in cases wherein bone discontinuity is extensive, surgical intervention...
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Cell shape characteristics of human skeletal muscle cells as a predictor of myogenic competency: A new paradigm towards precision cell therapy J. Tissue Eng. (IF 8.2) Pub Date : 2023-03-16 Charlotte Desprez, Davide Danovi, Charles H Knowles, Richard M Day
Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous...
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A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods J. Tissue Eng. (IF 8.2) Pub Date : 2023-03-08 Soraya Williams, Aveen R Jalal, Mark P Lewis, Owen G Davies
Extracellular vesicles (EVs) continue to gain interest across the scientific community for diagnostic and therapeutic applications. As EV applications diversify, it is essential that researchers ar...
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Preclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review J. Tissue Eng. (IF 8.2) Pub Date : 2023-02-27 Elena Stocco, Silvia Barbon, Marco Mammana, Giovanni Zambello, Martina Contran, Pier Paolo Parnigotto, Veronica Macchi, Maria Teresa Conconi, Federico Rea, Raffaele De Caro, Andrea Porzionato
Severe tracheal injuries that cannot be managed by mobilization and end-to-end anastomosis represent an unmet clinical need and an urgent challenge to face in surgical practice; within this scenari...
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Position Paper Progress in the development of biomimetic engineered human tissues J. Tissue Eng. (IF 8.2) Pub Date : 2023-02-27 Umber Cheema
Tissue engineering (TE) is the multi-disciplinary approach to building 3D human tissue equivalents in the laboratory. The advancement of medical sciences and allied scientific disciplines have aspi...