-
Highly chromophoric fluorescent-labeled methionyl-initiator tRNAs applicable in living cells Biotechnol. J. (IF 4.7) Pub Date : 2024-03-17 Jung Min Kim, Jinho Jung
-
Recent advances and fundamentals of microalgae cultivation technology Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Julian M. Rozenberg, Boris A. Sorokin, Amina N. Mukhambetova, Anna A. Emelianova, Valentin V. Kuzmin, Oxana Y. Belogurova‐Ovchinnikova, Denis V. Kuzmin
-
Rigid linker peptides improve the stability and anti‐inflammation effect of human serum albumin and α‐melanocyte‐stimulating hormone fusion proteins Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yiyao Liu, Yang Li, Xueyan Wei, Inam Ullah, Shahab Uddin, Jiatao Wang, Runjie Xia, MeiZhu Wang, Hui Yang, Hongyu Li
-
Predicting protein retention in ion‐exchange chromatography using an open source QSPR workflow Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Tim Neijenhuis, Olivier Le Bussy, Geoffroy Geldhof, Marieke E. Klijn, Marcel Ottens
-
Modification of nitrilase based on computer screening and efficient biosynthesis of 4‐cyanobenzoic acid Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yi‐Zhong Jin, Ji‐Dong Shen, Yu‐xin Wang, Hong‐Ming Shao, Hai‐Jian Xia, Jiao‐Hua Lin, Zhi‐Qiang Liu, Yu‐Guo Zheng
-
Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Qing Li, Jianyu Zhu, Ting Yuan, Leina Dou, Xuezhi Yu, Jianzhong Shen, Huijuan Yang, Zhanhui Wang
-
Tuning capsid formation dynamics in recombinant adeno‐associated virus producing synthetic cell lines to enhance full particle productivity Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Min Lu, Yu‐Chieh Lin, Han‐Jung Kuo, Wen Cai, Qian Ye, Liang Zhao, Wei‐Shou Hu
-
Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Wenhan Xiao, Xiangliu Shi, Haowei Huang, Xiaogang Wang, Wenshu Liang, Jianguo Xu, Fei Liu, Xiaomei Zhang, Guoqiang Xu, Jinsong Shi, Zhenghong Xu
-
Promoter screening and identification for metabolic regulation in Acremonium chrysogenum Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Ling Liu, Runge Li, Xinrun Zhang, Zhen Chen, Ali Mohsin, Haifeng Hang, Xiwei Tian, Ju Chu
-
Enhancing the production of adeno‐associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design‐of‐experiment optimization of triple plasmid ratio Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Sungje Park, Seunghyeon Shin, Haeshin Lee, Jae‐Hyung Jang, Gyun Min Lee
-
Standardized approach for accurate and reliable model development of ion‐exchange chromatography based on parameter‐by‐parameter method and consideration of extra‐column effects Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yu‐Cheng Chen, Hui‐Li Lu, Rong‐Zhu Wang, Guo Sun, Xue‐Qin Zhang, Jing‐Qi Liang, Alois Jungbauer, Shan‐Jing Yao, Dong‐Qiang Lin
-
Combining directed evolution with high cell permeability for high‐level cadaverine production in engineered Escherichia coli Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Xuemei Liu, Ruoshi Luo, Dan Wang, Kaixing Xiao, Fanzhen Lin, Ya Qi Kang, Xue Xia, Xiaojie Zhou, Ge Hu
-
-
Computer‐aided design to enhance the stability of aldo‐keto reductase KdAKR Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Chen Dai, Jia‐Xin Tian, Yu‐Feng Chen, Yue‐Han Ni, Lei Cui, Hai‐Xing Cao, Lin‐Lin Song, Shen‐Yuan Xu, Ya‐Jun Wang, Yu‐Guo Zheng
The aldo‐keto reductase (AKR) KdAKR from Kluyvermyces dobzhanskii can reduce t‐butyl 6‐chloro‐(5S)‐hydroxy‐3‐oxohexanoate ((5S)‐CHOH) to t‐butyl 6‐chloro‐(3R,5S)‐dihydroxyhexanoate ((3R,5S)‐CDHH), which is the key chiral intermediate of rosuvastatin. Herein, a computer‐aided design that combined the use of PROSS platform and consensus design was employed to improve the stability of a previously constructed
-
A single‐cell genomic strategy for alternative transcript start sites identification Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Yanling Peng, Qitong Huang, Danli Liu, Siyuan Kong, Rui Kamada, Keiko Ozato, Yubo Zhang, Jun Zhu
Alternative transcription start sites (TSSs) usage plays a critical role in gene transcription regulation in mammals. However, precisely identifying alternative TSSs remains challenging at the genome‐wide level. We report a single‐cell genomic technology for alternative TSSs annotation and cell heterogeneity detection. In the method, we utilize Fluidigm C1 system to capture individual cells of interest
-
Enhancing short‐chain fatty acids production via acidogenic fermentation of municipal sewage sludge: Effect of sludge characteristics and peroxydisulfate pre‐oxidation Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Faxin Liu, Wen Cheng, Jianping Xu, Tian Wan, Min Wang, Jiehui Ren, Maomao Ning, Hui Zhang, Xiaoping Zhou
This study first employed a combined pretreatment of low‐dose peroxy‐disulfate (PDS) and initial pH 10 to promote short‐chain fatty acids (SCFAs) production via acidogenic fermentation using different types of sewage sludge as substrates. The experimental results showed that the yield of maximal SCFAs and acetate proportion after the combined pretreatment were 1513.82 ± 28.25 mg chemical oxygen demand
-
Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Aastha Mittal, Neelam Mahala, Nikhil Hanamant Dhanawade, Sunil Kumar Dubey, Uma S. Dubey
Sorafenib, a multikinase inhibitor is used to treat hepatocellular and renal carcinoma. However, a low solubility impedes its bioavailability and thus, effectiveness. This study aims to enhance its effectiveness by using novel camel milk casein nanoparticles as a delivery system. This study evaluates the cytotoxicity of sorafenib encapsulated in camel milk casein nanoparticles against human hepatocarcinoma
-
High yield sterile filtration process for highly concentrated lentiviral vectors Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Jessica E. Vogel, Monica Terrao, Sarah Schwingal, Laura Kapitza, Daniel Brigulla, Vicky Pirzas, Holger Laux, Tobias Brandt
-
Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Yifei Zhang, Wei Zhang, Guangzheng Ma, Binbin Nian, Yi Hu
-
Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Lin‐Hui Yang, Meng‐zhen Liu, Zi‐Lei Chen, Ling‐Ling Tong, Dong‐Sheng Guo
-
Human proinsulin production in the milk of transgenic cattle Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Paulo S. Monzani, Juliano R. Sangalli, Rafael V. Sampaio, Samuel Guemra, Renato Zanin, Paulo R. Adona, Maria A. Berlingieri, Luiz F. C. Cunha‐Filho, Irma Y. Mora‐ Ocampo, Carlos P. Pirovani, Flávio V. Meirelles, Matthew B. Wheeler, Otavio M. Ohashi
-
Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Huimin Huang, Shumin Zhang, Yuanyuan Zhao, Ruisheng Xu, Wen‐song Tan, Haibo Cai
-
A large‐scale production of mesenchymal stem cells and their exosomes for an efficient treatment against lung inflammation Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Jinsong Zhang, Ruyi Lin, Yingyu Li, Jiawen Wang, Huiqing Ding, Panfeng Fang, Yingzhi Huang, Jing Shi, Jianqing Gao, Tianyuan Zhang
Mesenchymal stem cells (MSCs) and their produced exosomes have demonstrated inherent capabilities of inflammation‐guided targeting and inflammatory modulation, inspiring their potential applications as biologic agents for inflammatory treatments. However, the clinical applications of stem cell therapies are currently restricted by several challenges, and one of them is the mass production of stem cells
-
Encapsulation of pristine and silica‐coated human adipose‐derived mesenchymal stem cells in gelatin colloidal hydrogels for tissue engineering and bioprinting applications Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Marta M. Maciel, Negar Hassani Besheli, Tiago R. Correia, João F. Mano, Sander C. G. Leeuwenburgh
-
Lung‐specific exosomes for doxorubicin delivery in lung adenocarcinoma therapy Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Fengqiang Yu, Yanxun Chen, Weiqiang Yi, Maohao Guan, Nanlong Lin, Yi Zhuo, Jianbo Lin, Fancai Lai
Doxorubicin (DOX) could be utilized to treat lung adenocarcinoma (LUAD), while dose‐limiting cardiotoxicity limits its clinical utilization. MDA‐MB‐231 cell‐derived exosomes show lung‐specific organotropism features. In this study, we aimed to explore the potential of MDA‐MB‐231 cell‐derived exosomes in DOX specific delivery to the lung. MDA‐MB‐231 cell‐derived exosomes were coincubated with to construct
-
A refined Uni‐vector prime editing system improves genome editing outcomes in mammalian cells Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Ching‐Hui Huang, Szu‐Ying Chiu, Yu‐Chi Chou, Kou‐Juey Wu
Prime editing is an advanced technology in CRISPR/Cas research with increasing numbers of improved methodologies. The original multi‐vector method hampers the efficiency and precision of prime editing and also has inherent difficulty in generating homozygous mutations in mammalian cells. To overcome these technical issues, we developed a Uni‐vector prime editing system, wherein the major components
-
Engineering cannabinoid production in Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Christina Schmidt, Marco Aras, Oliver Kayser
Phytocannabinoids are natural products with highly interesting pharmacological properties mainly produced by plants. The production of cannabinoids in a heterologous host system has gained interest in recent years as a promising alternative to production from plant material. However, the systems reported so far do not achieve industrially relevant titers, highlighting the need for alternative systems
-
Overexpression of Klotho gene using CRISPR/Cas9 induces apoptosis and inhibits cell motility in the human colorectal cancer cells Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Merve Nur Soykan, Sibel Gunes
-
Effect of extracellular matrices on production and potency of mesenchymal stem cell‐derived exosomes Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Rittika Somadder, Lina Faraj, Saurav Datta, Mathumai Kanapathipillai, Gargi Ghosh
-
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Diem Do, Mümine Guruk, Małgorzata Kus‐Liśkiewicz, Christian Damblon, Anthony Arguelles‐Arias, Huseyin Erten, Patrick Fickers
-
Tracing endogenous proteins in living cells through electrotransfer of mRNA encoding chromobodies Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Théo Juncker, Ludovic Richert, Murielle Masson, Guy Zuber, Bruno Chatton, Mariel Donzeau
-
CRISPR/Cas system: A revolutionary tool for crop improvement Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Ayushi Mishra, Veda P. Pandey
-
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Hefeng Chen, Ran Liu, Shengliang Cai, Yingjiao Zhang, Chaoyi Zhu, Hao Yu, Shuang Li
-
Recent advances in applying omic technologies for studying acetic acid bacteria in industrial vinegar production: A comprehensive review Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Juan J. Román‐Camacho, Juan C. Mauricio, Inés María Santos‐Dueñas, Teresa García‐Martínez, Isidoro García‐García
-
The immune system of prokaryotes: potential applications and implications for gene editing Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Siyang Liu, Hongling Liu, Xue Wang, Lei Shi
-
MCM2 is involved in subtyping and tamoxifen resistance of ERα‐positive breast cancer by acting as the downstream factor of ERα Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Sainan Liu, Zhuoshuai Liang, Yujian Wang, Yaxuan Ren, Yulu Gu, Yichun Qiao, Huan He, Yong Li, Yi Cheng, Yawen Liu
-
Implications of carbon catabolite repression for Aspergillus‐based cell factories: A review Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Zhen‐Dong Wang, Bao‐Teng Wang, Long Jin, Hong‐Hua Ruan, Feng‐Jie Jin
-
Innovative translational platforms for rapid developing clinical vaccines against COVID‐19 and other infectious disease Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Ning Wang, Ting Wang
-
Duplication of a chromosome 2 segment in production CHO cell lines correlates with age‐related growth improvement Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Wei Wei, Laura Zielewicz, Lin Zhang
-
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Alba López‐Fernández, Víctor Garcia‐Gragera, Martí Lecina, Joaquim Vives
-
A highly sensitive and specific Homo1‐based real‐time qPCR method for quantification of human umbilical cord mesenchymal stem cells in rats Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Jing He, Zhangfan Wang, Chunchun Ao, Chengshu Tu, Yaqi Zhang, Cheng Chang, Cuihong Xiao, E Xiang, Wei Rao, Changyong Li, Dongcheng Wu
-
3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Muhammad Zafar Irshad Khan, Dildar Khan, Muhammad Yasir Akbar, Hao Wang, Ihsan‐ul Haq, Jian‐Zhong Chen
-
CFD evaluation of hydrophobic feedstock bench‐scale fermenters for efficient high agitation volumetric mass transfer Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Richard Marx, Huolong Liu, Seongkyu Yoon, Dongming Xie
-
Removal of phenolic compounds from sugarcane syrup and impact on Saccharomyces cerevisiae fermentation for β‐farnesene production Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Luís Carlos Carvalho, Ana L. S. Oliveira, Erdem Carsanba, Ana Lopes, Tânia Leal, Mónica Ribeiro, Sara Fernandes, Manuela Pintado, Carla Oliveira
-
Engineering CREB‐activated promoters for adenosine‐induced gene expression Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 John Robert Cox, Andrea Fox, Conor Lenahan, Liza Pivnik, Matthew Manion, John Blazeck
-
Filtration of a multi‐serotype glycoconjugate vaccine drug product through sterilizing grade 0.2/0.22 µm pore size filters Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Zhuoshi Du, Siyi Qiu, Kanwal Gill, Vishal Toprani, Andrew L. Zydney
-
High throughput generating stable spheroids with tip‐refill wafer Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Xiaoyan Yang, Rong Pan, Ke Ning, Yuanyuan Xie, Feng Chen, Wei Sun, Ling Yu
-
Cryopreservation of organoids: Strategies, innovation, and future prospects Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Hengxin Han, Taijie Zhan, Ning Guo, Mengdong Cui, Yi Xu
-
Liamocins overproduction via the two‐pH stage fermentation and anti‐Aspergillus flavus activity of Massoia lactone Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Peng Wang, Mei Zhang, Shou‐Feng Zhao, Zhao‐Rui Zhang, Guang‐Lei Liu, Zhe Chi, Zhen‐Ming Chi
-
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Mingxia Liang, Fanlei Ran, Li Li, Haiying Hang, Lili An
-
Screening of low‐toxic zinc oxide nanomaterials and study the apoptosis mechanism of NSC‐34 cells Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Yuanyuan Li, Yan Lu, Jingjing Li, Mei Li, Huitian Gou, Xiaolin Sun, Xiaoli Xu, Beibei Song, Zhiyu Li, Yonghua Ma
-
Pipette‐free field‐deployable molecular diagnostic kit for bimodal visual detection of infectious RNA viruses Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 So Yeon Park, Kieu The Loan Trinh, Yoon‐Jae Song, Nae Yoon Lee
-
Drug‐loaded lipid nanoparticles improve the removal rates of the Staphylococcus aureus biofilm Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Jiaju Qiao, Antuo Hu, Haibo Zhou, Zhaoxin Lu, Fanqiang Meng, Changzheng Shi, Xiaomei Bie
-
The choice of ultra‐low attachment plates impacts primary human and primary canine hepatocyte spheroid formation, phenotypes, and function Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Chen Xing, Aurino Kemas, Evgeniya Mickols, Kathrin Klein, Per Artursson, Volker M. Lauschke
-
Exploring metabolic responses and pathway changes in CHO‐K1 cells under varied aeration conditions and copper supplementations using 1H NMR‐based metabolomics Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Yingting Shi, Yuxiang Wan, Yan Sun, Jiayu Yang, Yuting Lu, Xinyuan Xie, Jianyang Pan, Haibin Wang, Haibin Qu
-
Secretory production and characterization of a highly effective chitosanase from Streptomyces coelicolor A3(2) M145 in Pichia pastoris Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Jiayu Liang, Shengbin He, Jian Sun, Haodong Bao, Lanyu Cui
-
Exploring engineering strategies that enhance de novo production of exotic cyclopropane fatty acids in Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Wei Jiang, Huadong Peng, Lizhong He, Rodrigo Lesma‐Amaro, Victoria S. Haritos
-
A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Siyuan Ma, Yuanyuan Ma
-
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Yongshuai Yang, Siyu Guo, Can‐Jian Hong, Zhao‐Xun Liang, Chun Loong Ho
-
Engineering and transcriptome study of Saccharomyces cerevisiae to produce ginsenoside compound K by glycerol Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Chuanbo Zhang, Jinping Tian, Jiale Zhang, Ruixia Liu, Xiaomeng Zhao, Wenyu Lu