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Cell‐free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block Biotechnol. J. (IF 3.912) Pub Date : 2020-11-27 Anna Schwarz; Sebastian Hecko; Florian Rudroff; Jeffrey T. Kohrt; Roger M. Howard; Margit Winkler
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Optimization of a transient antibody expression platform towards high titer and efficiency Biotechnol. J. (IF 3.912) Pub Date : 2020-11-23 Elizabeth Greene; Daniela Cazacu; Ninkka Tamot; Steven Castellano; Akshita Datar; Anthony Kronkaitis; Douglas Gebhard; Jon Reed; Paul Mawson; Lore Florin; Nicholas Rossi; Anthony Lauer; Laura Juckem; Andrew Nixon; Till Wenger; Saurabh Sen
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CRISPR/Ca12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction Biotechnol. J. (IF 3.912) Pub Date : 2020-12-23 Patrick G. Schweickert; Ning Wang; Stephanie L. Sandefur; Michael E. Lloyd; Stephen F. Konieczny; Christopher. C. Frye; Zhuo Cheng
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Gamma irradiating chromatography columns enables bioburden‐free integrated continuous biomanufacturing Biotechnol. J. (IF 3.912) Pub Date : 2020-12-13 Chad Varner; Rohan Patil; Rahul Godawat; Veena Warikoo; Konstantin Konstantinov; Kevin P. Brower
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Advancements in molecular design and bioprocessing of recombinant adeno‐associated virus gene delivery vectors using the insect‐cell baculovirus expression platform Biotechnol. J. (IF 3.912) Pub Date : 2020-12-04 Pranav R.H. Joshi; Alina Venereo‐Sanchez; Parminder S. Chahal; Amine A. Kamen
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A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter Biotechnol. J. (IF 3.912) Pub Date : 2020-11-28 Patrick Opdensteinen; Aleksandr Lobanov; Johannes Felix Buyel
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Process economics evaluation of cell‐free synthesis for the commercial manufacture of antibody drug conjugates Biotechnol. J. (IF 3.912) Pub Date : 2020-11-24 Christos Stamatis; Suzanne S. Farid
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Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine Biotechnol. J. (IF 3.912) Pub Date : 2021-01-25 Marna Eliana Sakalem; Maria Teresa De Sibio; Felipe Allan da Silva da Costa; Miriane de Oliveira
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Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate Biotechnol. J. (IF 3.912) Pub Date : 2020-12-21 Hao Wang; Lin Zhang; Yang Wang; Jianghua Li; Guocheng Du; Zhen Kang
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[Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva] Biotechnol. J. (IF 3.912) Pub Date : 2021-01-23 Wentao Zheng; Xue Wang; Yuwei Chen; Yachao Dong; Diao Zhou; Ruxin Liu; Haibo Zhou; Xiaoying Bian; Hailong Wang; Qiang Tu; Vinothkannan Ravichandran; Youming Zhang; Aiying Li; Jun Fu; Jia Yin
Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01‐1 is a broad‐spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting,
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Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies Biotechnol. J. (IF 3.912) Pub Date : 2021-01-23 Marcus Weinguny; Gerald Klanert; Peter Eisenhut; Isac Lee; Winston Timp; Nicole Borth
Chinese hamster ovary (CHO) cells are the most extensively used mammalian production system for biologics intended for use in humans. A critical step in the establishment of production cell lines is single cell cloning, with the objective of achieving high productivity and product quality. Despite general use, knowledge of the effects of this process is limited. Importantly, single cell cloned cells
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Lyophilization stabilizes clinical‐stage core‐crosslinked polymeric micelles to overcome cold chain supply challenges Biotechnol. J. (IF 3.912) Pub Date : 2021-01-23 Tarun Ojha; Qizhi Hu; Claudio Colombo; Jan Wit; Michiel van Geijn; Mies J. van Steenbergen; Mahsa Bagheri; Hiltrud Königs‐Werner; Eva Miriam Buhl; Ruchi Bansal; Yang Shi; Wim E. Hennink; Gert Storm; Cristianne J. F. Rijcken; Twan Lammers
CriPecࣨ technology enables the generation of drug‐entrapped biodegradable core‐crosslinked polymeric micelles (CCPM) with high drug loading capacity, tailorable size and drug release kinetics. Docetaxel‐entrapped CCPM, also referred to as CPC634, have demonstrated favorable pharmacokinetics, tolerability and enhanced tumor uptake in patients. Clinical efficacy evaluation is ongoing. CPC634 is currently
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Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana Biotechnol. J. (IF 3.912) Pub Date : 2021-01-22 Alexandra Castilho; Jennifer Schwestka; Nikolaus F. Kienzl; Ulrike Vavra; Clemens Grünwald‐Gruber; Shiva Izadi; Chaitra Hiremath; Janine Niederhöfer; Elisabeth Laurent; Vanessa Monteil; Ali Mirazimi; Gerald Wirnsberger; Johannes Stadlmann; Eva Stöger; Lukas Mach; Richard Strasser
Human angiotensin‐converting enzyme 2 (ACE2) is the primary host cell receptor for Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) binding and cell entry. Administration of high concentrations of soluble ACE2 can be utilized as a decoy to block the interaction of the virus with cellular ACE2 receptors and potentially be used as a strategy for treatment or prevention of coronavirus disease
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Novel erythropoietin‐based therapeutic candidates with extra N‐glycan sites that block hematopoiesis but preserve neuroplasticity Biotechnol. J. (IF 3.912) Pub Date : 2021-01-20 Milagros Bürgi; Gabriela Inés Aparicio; Aquiles Dorella; Ricardo Kratje; Camila Scorticati; Marcos Oggero
Neurological disorders affect millions of people worldwide causing behavior‐cognitive disabilities. Nowadays there is no effective treatment for them. Human erythropoietin (hEPO) has been used in clinical tests because of its neurotrophic and cytoprotective properties. However, the erythropoietic activity (EA) should be considered as a side effect. Some analogs like asialoEPO, carbamylated‐EPO or EPO‐peptides
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Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3‐D spheroids Biotechnol. J. (IF 3.912) Pub Date : 2021-01-20 Marta H. G. Costa; Joana Serra; Todd C. McDevitt; Joaquim M. S. Cabral; Cláudia L. da Silva; Frederico Castelo Ferreira
Strategies aiming at increasing the survival and paracrine activity of human mesenchymal stromal cells (MSCs) are of utmost importance to achieve the full therapeutic potential of these cells. Herein, we propose both physical and biochemical strategies to enhance the survival, homing, angiogenic and immunomodulatory properties of MSCs in vitro. To that purpose, we compared the effect of exposing either
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Mixing and oxygen transfer characteristics of a microplate bioreactor with surface‐attached microposts Biotechnol. J. (IF 3.912) Pub Date : 2021-01-19 Justin T. Fisher; Travis O. Gurney; Brittany M. Mason; Jay K. Fisher; William J. Kelly
Bioprocess optimization for cell‐based therapies is a resource heavy activity. To reduce the associated cost and time, process development may be carried out in small volume systems, with the caveat that such systems be predictive for process scaleup. The transport of oxygen from the gas phase into the culture medium, characterized using the volumetric mass transfer coefficient, kLa, has been identified
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A novel strategy of selective gene delivery by using a uniform magnetic field Biotechnol. J. (IF 3.912) Pub Date : 2020-11-23 Minori Dateki; Osamu Imamura; Masaaki Arai; Hidehisa Shimizu; Kunio Takishima
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A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis Biotechnol. J. (IF 3.912) Pub Date : 2021-01-02 Yeji Kim; Jae Yong Ryu; Hyun Uk Kim; Woo Dae Jang; Sang Yup Lee
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Outside Front Cover: (Biotechnology Journal 1/2021) Biotechnol. J. (IF 3.912) Pub Date : 2021-01-08 Mariana V. Ferreira; Elisa T. Cabral; Ana Sofia Coroadinha
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Biomanufacturing of Gene Therapy Vectors Biotechnol. J. (IF 3.912) Pub Date : 2021-01-08 Cristina Peixoto; Otto‐Wilhelm Merten
The progress from clinical development toward product licensure of several programs presents challenges mainly to gene therapy product manufacturing. These include challenges in viral vector‐manufacturing capacity but also in process variability, difficulty characterizing complex materials, and lack of knowledge of critical process parameters and their effect on critical quality attributes of the viral
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Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy Biotechnol. J. (IF 3.912) Pub Date : 2020-11-04 J. Fraser Wright
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Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity Biotechnol. J. (IF 3.912) Pub Date : 2021-01-07 Fabio Pasin
Synthetic genomics‐driven dematerialization of genetic resources facilitates flexible hypothesis testing and rapid product development. Biological sequences have compositional biases, which, I reasoned, could be exploited for engineering of enhanced synthetic genomics systems. In proof‐of‐concept assays reported herein, the abundance of random oligonucleotides in viral genomic components was analyzed
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Error propagation in constraint‐based modeling of Chinese hamster ovary cells Biotechnol. J. (IF 3.912) Pub Date : 2020-12-19 Diana Széliová; Dmytro Iurashev; David E Ruckerbauer; Gunda Koellensperger; Nicole Borth; Michael Melcher; Jürgen Zanghellini
Chinese hamster ovary (CHO) cells are the most popular mammalian cell factories for the production of glycosylated biopharmaceuticals. To further increase titer and productivity and ensure product quality, rational system‐level engineering strategies based on constraint‐based metabolic modeling, such as flux balance analysis (FBA), have gained strong interest. However, the quality of FBA predictions
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Comprehensive characterization of dihydrofolate reductase‐mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies Biotechnol. J. (IF 3.912) Pub Date : 2020-12-13 Sang Yoon Lee; Minhye Baek; Gyun Min Lee
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Key advances in biocatalytic phosphorylations in the last two decades: Biocatalytic syntheses in vitro and biotransformations in vivo (in humans) Biotechnol. J. (IF 3.912) Pub Date : 2020-11-30 Roland Wohlgemuth
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Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost‐effective method for expression of complex molecules Biotechnol. J. (IF 3.912) Pub Date : 2020-12-01 Emily Dong; Cynthia Lam; Danming Tang; Salina Louie; Mandy Yim; Ambrose J. Williams; William Sawyer; Shirley Yip; Joseph Carver; Ali AlBarakat; Joni Tsukuda; Brad Snedecor; Shahram Misaghi
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Accelerating HIV‐1 VLP production using stable High Five insect cell pools Biotechnol. J. (IF 3.912) Pub Date : 2020-11-28 Eduard Puente‐Massaguer; Paula Grau‐Garcia; Florian Strobl; Reingard Grabherr; Gerald Striedner; Martí Lecina; Francesc Gòdia
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Selective ene‐reductase immobilization to magnetic nanoparticles through a novel affinity tag Biotechnol. J. (IF 3.912) Pub Date : 2020-11-27 Alexander A. Zanker; Nadim Ahmad; Tuan Hoang Son; Sebastian P. Schwaminger; Sonja Berensmeier
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Dissolvable Gelatin‐Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells Biotechnol. J. (IF 3.912) Pub Date : 2020-10-13 Ee Xien Ng; Ming Wang; Shu Hui Neo; Ching Ann Tee; Chia‐Hung Chen; Krystyn J. Van Vliet
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Outside Front Cover: (Biotechnology Journal 12/2020) Biotechnol. J. (IF 3.912) Pub Date : 2020-12-07 Arvie Camille V. de Guzman; Md. Abdur Razzak; Batmagnai Purevdulam; Shin Sik Choi
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Nanomaterials for Biomedical Applications Biotechnol. J. (IF 3.912) Pub Date : 2020-12-07 Mariana B. Oliveira; Feng Li; Jonghoon Choi; João F. Mano
Mariana B. Oliveira Feng Li Jonghoon Choi João F. Mano Nanotechnology has enabled the development of cutting‐edge applications in the fields of biotechnology and bioengineering. Such an impact has emerged in a plethora of technologies and devices targeted at more effective disease diagnosis, monitoring, and treatment. In this special issue, nanostructured materials in different shapes and assembly
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Assessing the Combinatorial Chemo‐Photothermal Therapy Mediated by Sulfobetaine Methacrylate‐Functionalized Nanoparticles in 2D and 3D In Vitro Cancer Models Biotechnol. J. (IF 3.912) Pub Date : 2020-10-15 Inês Mó; Cátia G. Alves; Duarte de Melo‐Diogo; Rita Lima‐Sousa; Ilídio J. Correia
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Dielectrophoretic Manipulation of Janus Particle in Conductive Media for Biomedical Applications Biotechnol. J. (IF 3.912) Pub Date : 2020-10-16 Minji Lee; Jong Bin Won; Dae Ho Jung; Jaekyoung Kim; Yonghyun Choi; Kubra Akyildiz; Jonghoon Choi; Kyobum Kim; Jiung Cho; Hyunsik Yoon; Hyung‐Jun Koo
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Biohydrogen production from microalgae – major bottlenecks and future research perspectives Biotechnol. J. (IF 3.912) Pub Date : 2020-11-29 Dillirani Nagarajan; Cheng‐Di Dong; Chun‐Yen Chen; Duu‐Jong Lee; Jo‐Shu Chang
The imprudent use of fossil fuels has resulted in high greenhouse gas (GHG) emissions, leading to climate change and global warming. Reduction in GHG emissions and energy insecurity imposed by the depleting fossil fuel reserves led to the search for alternative sustainable fuels. Hydrogen is a potential alternative energy carrier and is of particular interest because hydrogen combustion releases only
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Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials Biotechnol. J. (IF 3.912) Pub Date : 2020-11-01 Kok Zhi Lee; Vindula Basnayake Pussepitiyalage; Yu‐Hsuan Lee; L. Sue Loesch‐Fries; Michael T. Harris; Shohreh Hemmati; Kevin V. Solomon
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Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae Biotechnol. J. (IF 3.912) Pub Date : 2020-11-01 Liang Sun; Yong‐Su Jin
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Illuminating the Regenerative Properties of Stem Cells In Vivo with Bioluminescence Imaging Biotechnol. J. (IF 3.912) Pub Date : 2020-10-22 Sean D. Madsen; Margaret K. Giler; Bruce A. Bunnell; Kim C. O'Connor
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Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers Biotechnol. J. (IF 3.912) Pub Date : 2020-10-01 Payel Datta; LuFeng Yan; Adeola Awofiranye; Jonathan S. Dordick; Robert J. Linhardt
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Outside Front Cover: (Biotechnology Journal 11/2020) Biotechnol. J. (IF 3.912) Pub Date : 2020-11-10 Lisa Schäfer; Katja Bühler; Rohan Karande; Bruno Bühler
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Molecular and Engineering Aspects of Biocatalysis Biotechnol. J. (IF 3.912) Pub Date : 2020-11-10 Roland Wohlgemuth; Bruno Bühler
The area of biocatalysis has been experiencing tremendous growth from its early foundations to its present state of the art and science. Cross‐inspiration and interconnections between the art and know‐how of different practical biocatalytic applications and the motivation for the creation of fundamental knowledge in the science of biocatalysis over time made problem‐solving feasible to successfully
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Pseudomonas as Versatile Aromatics Cell Factory Biotechnol. J. (IF 3.912) Pub Date : 2020-09-25 Tobias Schwanemann; Maike Otto; Nick Wierckx; Benedikt Wynands
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Chemoenzymatic Total Syntheses of Artonin I with an Intermolecular Diels–Alderase Biotechnol. J. (IF 3.912) Pub Date : 2020-10-01 Xiaojing Liu; Jun Yang; Lei Gao; Liyun Zhang; Xiaoguang Lei
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Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs Biotechnol. J. (IF 3.912) Pub Date : 2020-10-20 Pao‐Chun Lin; Ren Liu; Krista Alvin; Shahreel Wahyu; Nicholas Murgolo; Jianxin Ye; Zhimei Du; Zhiwei Song
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Emerging Strategies in Anticancer Combination Therapy Employing Silica‐Based Nanosystems Biotechnol. J. (IF 3.912) Pub Date : 2020-10-20 Rafael R. Castillo; Maria Vallet‐Regí
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Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining Biotechnol. J. (IF 3.912) Pub Date : 2020-10-20 Adrian Elter; Jan P. Bogen; Steffen C. Hinz; David Fiebig; Arturo Macarrón Palacios; Julius Grzeschik; Björn Hock; Harald Kolmar
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Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates Biotechnol. J. (IF 3.912) Pub Date : 2020-10-21 Mariela P. Mezzina; María Tsampika Manoli; M. Auxiliadora Prieto; Pablo I. Nikel
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Adaptive Evolution in Producing Microtiter Cultivations Generates Genetically Stable Escherichia coli Production Hosts for Continuous Bioprocessing Biotechnol. J. (IF 3.912) Pub Date : 2020-10-20 Artur Schuller; Monika Cserjan‐Puschmann; Christoph Köppl; Reingard Grabherr; Martin Wagenknecht; Matteo Schiavinato; Juliane C. Dohm; Heinz Himmelbauer; Gerald Striedner
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Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV‐1 Infection Biotechnol. J. (IF 3.912) Pub Date : 2020-10-25 Marianna Romito; Alexandre Juillerat; Yik Lim Kok; Markus Hildenbeutel; Manuel Rhiel; Geoffroy Andrieux; Johannes Geiger; Carsten Rudolph; Claudio Mussolino; Aymeric Duclert; Karin J. Metzner; Philippe Duchateau; Toni Cathomen; Tatjana I. Cornu
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Advances in Lentivirus Purification Biotechnol. J. (IF 3.912) Pub Date : 2020-10-22 Ana Sofia Moreira; David Guia Cavaco; Tiago Q. Faria; Paula M. Alves; Manuel J. T. Carrondo; Cristina Peixoto
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Intensified Downstream Processing of Monoclonal Antibodies Using Membrane Technology Biotechnol. J. (IF 3.912) Pub Date : 2020-10-01 Sathish Nadar; Gary Shooter; Balaji Somasundaram; Evan Shave; Kym Baker; Linda H. L. Lua
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The SSV‐Seq 2.0 PCR‐Free Method Improves the Sequencing of Adeno‐Associated Viral Vector Genomes Containing GC‐Rich Regions and Homopolymers Biotechnol. J. (IF 3.912) Pub Date : 2020-10-16 Emilie Lecomte; Sylvie Saleun; Mathieu Bolteau; Aurélien Guy‐Duché; Oumeya Adjali; Véronique Blouin; Magalie Penaud‐Budloo; Eduard Ayuso
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Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production Biotechnol. J. (IF 3.912) Pub Date : 2020-10-16 Aurélien Jacob; Laurie Brun; Paloma Jiménez Gil; Lucie Ménard; Mohammed Bouzelha; Frédéric Broucque; Aline Roblin; Luk H. Vandenberghe; Oumeya Adjali; Cécile Robin; Achille François; Véronique Blouin; Magalie Penaud‐Budloo; Eduard Ayuso
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Localized Sampling Enables Monitoring of Cell State via Inline Electrospray Ionization Mass Spectrometry Biotechnol. J. (IF 3.912) Pub Date : 2020-09-24 Mason A. Chilmonczyk; Gilad Doron; Peter A. Kottke; Austin L. Culberson; Kelly Leguineche; Robert E. Guldberg; Edwin M. Horwitz; Andrei G. Fedorov
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Inhibition of E. coli host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness. Biotechnol. J. (IF 3.912) Pub Date : 2020-09-11 Jens Kastenhofer,Lukas Rettenbacher,Lukas Feuchtenhofer,Juergen Mairhofer,Oliver Spadiut
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Multimodal Imaging and Soft X‐Ray Tomography of Fluorescent Nanodiamonds in Cancer Cells Biotechnol. J. (IF 3.912) Pub Date : 2020-09-24 Philipp Reineck; Amanda N. Abraham; Arpita Poddar; Ravi Shukla; Hiroshi Abe; Takeshi Ohshima; Brant C. Gibson; Chaitali Dekiwadia; José J. Conesa; Eva Pereiro; Amy Gelmi; Gary Bryant
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Isolation of Common Light Chain Antibodies from Immunized Chickens Using Yeast Biopanning and Fluorescence-Activated Cell Sorting. Biotechnol. J. (IF 3.912) Pub Date : 2020-09-10 Jan P Bogen,Juliana Storka,Desislava Yanakieva,David Fiebig,Julius Grzeschik,Björn Hock,Harald Kolmar
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The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type Biotechnol. J. (IF 3.912) Pub Date : 2020-09-25 Bojana Radoman; Clemens Grünwald‐Gruber; Bernhard Schmelzer; Domen Zavec; Brigitte Gasser; Friedrich Altmann; Diethard Mattanovich
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Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes. Biotechnol. J. (IF 3.912) Pub Date : 2020-09-15 Damiano Totaro,Bojana Radoman,Bernhard Schmelzer,Mario Rothbauer,Matthias G Steiger,Torsten Mayr,Michael Sauer,Peter Ertl,Diethard Mattanovich
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Outside Front Cover: (Biotechnology Journal 10/2020) Biotechnol. J. (IF 3.912) Pub Date : 2020-10-06 Daisuke Sasaki; Kengo Sasaki; Akihiko Kondo
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Inside Front Cover: (Biotechnology Journal 10/2020) Biotechnol. J. (IF 3.912) Pub Date : 2020-10-06 Zhao Liu; Can‐Jian Hong; Yongshuai Yang; Lei Dai; Chun Loong Ho