样式: 排序: IF: - GO 导出 标记为已读
-
Construction and applications of the EOMA spheroid model of Kaposiform hemangioendothelioma J. Biol. Eng. (IF 5.6) Pub Date : 2024-03-14 Yanan Li, Xinglong Zhu, Li Li, Chunjuan Bao, Qin Liu, Ning zhang, Ziyan He, Yi Ji, Ji Bao
Kaposiform hemangioendothelioma (KHE) is a rare intermediate vascular tumor with unclear pathogenesis. Recently, three dimensional (3D) cell spheroids and organoids have played an indispensable role in the study of many diseases, such as infantile hemangioma and non-involuting congenital hemangiomas. However, few research on KHE are based on the 3D model. This study aims to evaluate the 3D superiority
-
Structural and practical identifiability analysis in bioengineering: a beginner’s guide J. Biol. Eng. (IF 5.6) Pub Date : 2024-03-04 Linda Wanika, Joseph R. Egan, Nivedhitha Swaminathan, Carlos A. Duran-Villalobos, Juergen Branke, Stephen Goldrick, Mike Chappell
Advancements in digital technology have brought modelling to the forefront in many disciplines from healthcare to architecture. Mathematical models, often represented using parametrised sets of ordinary differential equations, can be used to characterise different processes. To infer possible estimates for the unknown parameters, these models are usually calibrated using associated experimental data
-
An innovative lab-scale production for a novel therapeutic DNA vaccine candidate against rheumatoid arthritis J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-27 Juan Long, Xiao Zhao, Fei Liang, Yang Zeng, Nan Liu, Yuying Sun, Yongzhi Xi
Recent therapeutic-plasmid DNA vaccine strategies for rheumatoid arthritis (RA) have significantly improved. Our pcDNA-CCOL2A1 vaccine is the most prominent and the first antigen-specific tolerising DNA vaccine with potent therapeutic and prophylactic effects compared with methotrexate (MTX), the current “gold standard” treatment for collagen-induced arthritis (CIA). This study developed a highly efficient
-
Chitosan as a promising materials for the construction of nanocarriers for diabetic retinopathy: an updated review J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-22 Yan Lv, Chenglei Zhai, Gang Sun, Yangfang He
Diabetic retinopathy (DR) is a condition that causes swelling of the blood vessels of the retina and leaks blood and fluids. It is the most severe form of diabetic eye disease. It causes vision loss in its advanced stage. Diabetic retinopathy is responsible for causing 26% of blindness. Very insufficient therapies are accessible for the treatment of DR. As compared to the conventional therapies, there
-
Proteomic analysis of decellularized mice liver and kidney extracellular matrices J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-22 Anna-Maria Diedrich, Assal Daneshgar, Peter Tang, Oliver Klein, Annika Mohr, Olachi A. Onwuegbuchulam, Sabine von Rueden, Kerstin Menck, Annalen Bleckmann, Mazen A. Juratli, Felix Becker, Igor M. Sauer, Karl H. Hillebrandt, Andreas Pascher, Benjamin Struecker
The extracellular matrix (ECM) is a three-dimensional network of proteins that encases and supports cells within a tissue and promotes physiological and pathological cellular differentiation and functionality. Understanding the complex composition of the ECM is essential to decrypt physiological processes as well as pathogenesis. In this context, the method of decellularization is a useful technique
-
Preparation of bilayer tissue-engineered polyurethane/poly-L-lactic acid nerve conduits and their in vitro characterization for use in peripheral nerve regeneration J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-22 Mehran Nabipour, Amir Mellati, Mozhgan Abasi, Somayeh Ebrahimi Barough, Ayoob Karimizade, Parnian Banikarimi, Elham Hasanzadeh
Due to loss of peripheral nerve structure and/or function resulting from trauma, accidents, and other causes, peripheral nerve injuries continue to be a major clinical problem. These injuries can cause partial or total loss of sensory, motor, and autonomic capabilities as well as neuropathic pain. PNI affects between 13 and 23 out of every 100,000 people annually in developed countries. Regeneration
-
Advanced biomanufacturing and evaluation of adeno-associated virus J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-15 Kai Chen, Seulhee Kim, Siying Yang, Tanvi Varadkar, Zhuoxin Zora Zhou, Jiashuai Zhang, Lufang Zhou, Xiaoguang Margaret Liu
Recombinant adeno-associated virus (rAAV) has been developed as a safe and effective gene delivery vehicle to treat rare genetic diseases. This study aimed to establish a novel biomanufacturing process to achieve high production and purification of various AAV serotypes (AAV2, 5, DJ, DJ8). First, a robust suspensive production process was developed and optimized using Gibco Viral Production Cell 2
-
The progressive trend of modeling and drug screening systems of breast cancer bone metastasis J. Biol. Eng. (IF 5.6) Pub Date : 2024-02-05 Hanieh Kolahi Azar, Maliheh Gharibshahian, Mohammadreza Rostami, Vahid Mansouri, Leila Sabouri, Nima Beheshtizadeh, Nima Rezaei
Bone metastasis is considered as a considerable challenge for breast cancer patients. Various in vitro and in vivo models have been developed to examine this occurrence. In vitro models are employed to simulate the intricate tumor microenvironment, investigate the interplay between cells and their adjacent microenvironment, and evaluate the effectiveness of therapeutic interventions for tumors. The
-
Enhancing osteogenic differentiation of dental pulp stem cells through rosuvastatin loaded niosomes optimized by Box-Behnken design and modified by hyaluronan: a novel strategy for improved efficiency J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-26 Zaynab Sadeghi Ghadi, Amin Asadi, Younes Pilehvar, Mozhgan Abasi, Pedram Ebrahimnejad
Bone tissue engineering necessitates a stem cell source capable of osteoblast differentiation and mineralized matrix production. Dental pulp stem cells (DPSCs), a subtype of mesenchymal stem cells from human teeth, present such potential but face challenges in osteogenic differentiation. This research introduces an innovative approach to bolster DPSCs’ osteogenic potential using niosomal and hyaluronan
-
Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-25 Stephany Celeste Gutiérrez-Ruíz, Hernán Cortes, Maykel González-Torres, Zainab M. Almarhoon, Eda Sönmez Gürer, Javad Sharifi-Rad, Gerardo Leyva-Gómez
Polymeric nanoparticles can be used for wound closure and therapeutic compound delivery, among other biomedical applications. Although there are several nanoparticle obtention methods, it is crucial to know the adequate parameters to achieve better results. Therefore, the objective of this study was to optimize the parameters for the synthesis, purification, and freeze-drying of chitosan nanoparticles
-
Poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)-Polyethylene glycol 20k(PEG20k) as a promising delivery system for PT2399 in the treatment of disc degeneration J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-22 Zhencong Li, Weilin Zhang, Shengbang Huang, Zhiwen Dai, Jinguo Liang, Qiulan Qiu, Siyuan Chen, Weixiong Guo, Zhongwei Wang, Jinsong Wei
Disc degeneration often leads to a highly prevalent symptom known as low back pain. Healthy nucleus pulposus tissue exhibited a hypoxic environment devoid of blood vessels, while degenerated nucleus pulposus experienced hypoxic deterioration and the formation of new blood vessels. In this study, the expression of important genes like HIF-2α was found to vary between normal and degenerated nucleus pulposus
-
Exploring the impact of taurine on the biochemical properties of urate oxidase: response surface methodology and molecular dynamics simulation J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-22 Parisa Shahmoradipour, Maryam Zaboli, Masoud Torkzadeh-Mahani
This paper investigates the impact of taurine as an additive on the structural and functional stability of urate oxidase. First, the effect of the processing parameters for the stabilization of Urate Oxidase (UOX) using taurine was examined using the response surface methodology (RSM) and the central composite design (CCD) model. Also, the study examines thermodynamic and kinetic parameters as well
-
An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-16 Takumi Kishimoto, Ken Nishimura, Kana Morishita, Aya Fukuda, Yusaku Miyamae, Yutaro Kumagai, Kimio Sumaru, Mahito Nakanishi, Koji Hisatake, Masayuki Sano
Viral vectors are attractive gene delivery vehicles because of their broad tropism, high transduction efficiency, and durable expression. With no risk of integration into the host genome, the vectors developed from RNA viruses such as Sendai virus (SeV) are especially promising. However, RNA-based vectors have limited applicability because they lack a convenient method to control transgene expression
-
Stable, neuron-specific gene expression in the mouse brain J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-16 Osama Ahmed, Kingsley M. Ekumi, Francesco V. Nardi, Gulimiheranmu Maisumu, Khaled Moussawi, Eric D. Lazartigues, Bo Liang, Abraam M. Yakoub
Gene delivery to, and expression in, the mouse brain is important for understanding gene functions in brain development and disease, or testing gene therapies. Here, we describe an approach to express a transgene in the mouse brain in a cell-type-specific manner. We use stereotaxic injection of a transgene-expressing adeno-associated virus into the mouse brain via the intracerebroventricular route
-
Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-16 Wenxuan Wang, Tingting Yang, Sihui Chen, Liying Liang, Yingxin Wang, Yin Ding, Wei Xiong, Xiuhong Ye, Yonglong Guo, Shuhao Shen, Hang Chen, Jiansu Chen
Retinal pigment epithelium (RPE) cell therapy is a promising way to treat many retinal diseases. However, obtaining transplantable RPE cells is time-consuming and less effective. This study aimed to develop novel strategies for generating engineered RPE patches with physiological characteristics. Our findings revealed that RPE cells derived from human induced pluripotent stem cells (hiPSCs) successfully
-
Kinematic movement and balance parameter analysis in neurological gait disorders J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-15 Chuh-Hyoun Na, Hannah Lena Siebers, Julia Reim, Jörg Eschweiler, Frank Hildebrand, Hans Clusmann, Marcel Betsch
Neurological gait disorders are mainly classified based on clinical observation, and therefore difficult to objectify or quantify. Movement analysis systems provide objective parameters, which may increase diagnostic accuracy and may aid in monitoring the disease course. Despite the increasing wealth of kinematic movement and balance parameter data, the discriminative value for the differentiation
-
An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-11 Long Xie, Zhenwen Huang, Meiyu Lan, Yaqi Cao, Lingling Sun, Lang Zhang, Erwei Zuo, Yangqing Lu
Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, we used site-specific integration of an EGFP fused to the OVAL gene by a rigid linker, (EAAAK)3, at the endogenous ovalbumin locus in chicken primordial germ
-
Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-11 Mohadeseh Haji Abdolvahab, Mojdeh Safari, Farkhonde Hasannejad, Nika Asefi, Alireza Salimi, Mahboobeh Nazari
The sequence of a carboxy-terminal of the β-lactam sensor-transducer protein (BlaR-CTD) from Bacillus licheniformis ATCC14580 was extracted from US7745193B2 patent and expressed in E. coli using pColdI vector as a soluble His-tag recombinant protein. In this study, several excipients were used to improve the stability of recombinant BlaR-CTD and obtain the optimal formulation for this protein using
-
Mussel-inspired electroactive, antibacterial and antioxidative composite membranes with incorporation of gold nanoparticles and antibacterial peptides for enhancing skin wound healing J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-11 Yongkang Dong, Zheng Wang, Jiapeng Wang, Xuedi Sun, Xiaoyu Yang, Guomin Liu
Large skin wounds are one of the most important health problems in the world. Skin wound repair and tissue regeneration are complex processes involving many physiological signals, and effective wound healing remains an enormous clinical challenge. Therefore, there is an urgent need for a strategy to rapidly kill bacteria, promote cell proliferation and accelerate wound healing. At present, electrical
-
Treatment of effluents from Food Services Establishment (FSEs) by physico-chemical processes: a case study for Trinidad & Tobago J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-05 Cudjoe Shamika, Banerjee Goutam, Cooper Vincent
Effluents from Food Services Establishments (FSEs) contain primarily Fats, Oil and Grease (FOG) which severely impact on sewers and the environment when released in high concentrations. In Trinidad & Tobago, it is estimated that approximately 231,304 kg/day of unaccounted for FOG bearing wastewaters from FSEs, are released into the environment with no viable treatment in the country. This research
-
3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects J. Biol. Eng. (IF 5.6) Pub Date : 2024-01-02 Chenglong Wang, Jinlei Dong, Fanxiao Liu, Nan Liu, Lianxin Li
The long-term nonunion of bone defects is always a difficult problem in orthopaedics treatment. Artificial bone implants made of polymeric materials are expected to solve this problem due to their suitable degradation rate and good biocompatibility. However, the lack of mechanical strength, low osteogenic induction ability and poor hydrophilicity of these synthetic polymeric materials limit their large-scale
-
3D printed zirconia used as dental materials: a critical review J. Biol. Eng. (IF 5.6) Pub Date : 2023-12-21 Guanyu Su, Yushi Zhang, Chunyu Jin, Qiyue Zhang, Jiarui Lu, Zengqian Liu, Qiang Wang, Xue Zhang, Jia Ma
In view of its high mechanical performance, outstanding aesthetic qualities, and biological stability, zirconia has been widely used in the fields of dentistry. Due to its potential to produce suitable advanced configurations and structures for a number of medical applications, especially personalized created devices, ceramic additive manufacturing (AM) has been attracting a great deal of attention
-
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures J. Biol. Eng. (IF 5.6) Pub Date : 2023-12-14 Michael Killinger, Adéla Kratochvilová, Eva Ingeborg Reihs, Eva Matalová, Karel Klepárník, Mario Rothbauer
Three-dimensional (3D) cell cultures are to date the gold standard in biomedical research fields due to their enhanced biological functions compared to conventional two-dimensional (2D) cultures. 3D cell spheroids, as well as organoids, are better suited to replicate tissue functions, which enables their use both as in vitro models for basic research and toxicology, as well as building blocks used
-
Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells J. Biol. Eng. (IF 5.6) Pub Date : 2023-12-07 Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier
Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic potential. Furthermore, expression of multiple transgenes in hMSCs could greatly advance their clinical significance for treatment of multifaceted diseases,
-
Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein J. Biol. Eng. (IF 5.6) Pub Date : 2023-12-04 Nan Zhou, TingWei Gu, Yang Xu, Yuda Liu, LiHua Peng
Nerve growth factor (NGF) is a vital cytokine that plays a crucial role in the development and regeneration of the nervous system. It has been extensively studied for its potential therapeutic applications in various neural diseases. However, as a protein drug, limited natural source seriously hinders its translation and clinical applications. Conventional extraction of NGF from mouse submandibular
-
Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-27 Chih-Yun Lee, Swathi Nedunchezian, Sung-Yen Lin, Yu-Feng Su, Che-Wei Wu, Shun-Cheng Wu, Chung-Hwan Chen, Chih-Kuang Wang
Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a promising solution for osteochondral tissue repair in this study. The subchondral bone scaffold approach is based
-
Preparation and in vitro evaluation of biological agents based on Zinc-laponite- curcumin incorporated in alginate hydrogel J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-24 Negar Karimi Hajishoreh, Mehdi Dadashpour, Abolfazl Akbarzadeh
Due to their outstanding structures and properties, three-dimensional (3D) hydrogels and nanoparticles have been widely studied and indicated a very high potential for medical, therapeutic, and diagnostic applications. However, hydrogels and nanoparticles systems have particular drawbacks that limit their widespread applications. In recent years, the incorporation of nanostructured systems into hydrogel
-
Research progress of biomimetic materials in oral medicine J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-23 Xinyu Luo, Jiayue Niu, Guanyu Su, Linxi Zhou, Xue Zhang, Ying Liu, Qiang Wang, Ningning Sun
Biomimetic materials are able to mimic the structure and functional properties of native tissues especially natural oral tissues. They have attracted growing attention for their potential to achieve configurable and functional reconstruction in oral medicine. Though tremendous progress has been made regarding biomimetic materials, significant challenges still remain in terms of controversy on the mechanism
-
Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-23 Meike Bielfeldt, Kai Budde-Sagert, Nikolai Weis, Maren Buenning, Susanne Staehlke, Julius Zimmermann, Nils Arbeiter, Sahba Mobini, María Ujué González, Henrike Rebl, Adelinde Uhrmacher, Ursula van Rienen, Barbara Nebe
Electrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The electrical stimulation of the
-
Follicular reconstruction and neo-oogenesis in xenotransplantation of human ovarian isolated cells derived from chemotherapy-induced POF patients J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-20 Sara Khaleghi, Farideh Eivazkhani, Somayeh Tavana, Ashraf Moini, Marefat Ghaffari Novin, Petkov Stoyan, Hamid Nazarian, Rouhollah Fathi
Developing new strategies to restore fertility in patients with chemotherapy-induced Premature Ovarian Failure (Chemo-POF) is important. We aimed to construct an Artificial Ovary (AO) by seeding Human Ovarian Cortical Cells (HOCCs) into Human ovarian Decellularized Cortical Tissue (DCT). We assessed the AO’s ability to produce new ovarian follicles following xenotransplantation to NMRI mice. The DCTs
-
Seaweed-based alginate/hydroxyapatite composite for the effective removal of bacteria, cyanobacteria, algae, and crystal violet from water J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-14 Mohamed Gomaa, Amal William Danial
A novel cost-effective and multifunctional nanocomposite was developed based on sustainable macroalgae biomass. The brown seaweed Sargassum latifolium was utilized for alginate extraction and the calcareous red seaweed Tricleocarpa fragilis was utilized as CaCO3 source for nanohydroxyapatite synthesis. The developed Zn2+-crosslinked alginate/nanohydroxyapatite (ZA/nHA) beads were characterized by FT-IR
-
Label-free multimodal electro-thermo-mechanical (ETM) phenotyping as a novel biomarker to differentiate between normal, benign, and cancerous breast biopsy tissues J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-13 Anil Vishnu G. K., Gayatri Gogoi, Midhun C. Kachappilly, Annapoorni Rangarajan, Hardik J. Pandya
Technologies for quick and label-free diagnosis of malignancies from breast tissues have the potential to be a significant adjunct to routine diagnostics. The biophysical phenotypes of breast tissues, such as its electrical, thermal, and mechanical properties (ETM), have the potential to serve as novel markers to differentiate between normal, benign, and malignant tissue. We report a system-of-biochips
-
Antler stem cell-derived exosomes promote regenerative wound healing via fibroblast-to-myofibroblast transition inhibition J. Biol. Eng. (IF 5.6) Pub Date : 2023-11-08 Guokun Zhang, Dongxu Wang, Jing Ren, Jiping Li, Qianqian Guo, Liyan Shi, Chunyi Li
The typical outcome of mammalian wound healing is scarring, a fibrotic process mediated by myofibroblast aggregation. Perfect healing in a clinical setting is relatively unexplored. Surprisingly, our previous studies have shown that the large wound (10 cm diameter or more) of the pedicle of deer naturally achieves regenerative restoration, realized through a paracrine pathway from adjacent antler stem
-
Dual-drug (Curcumin/Ciprofloxacin) loading and release from chitosan-based hydrogels embedded with magnetic Montmorillonite/Hyaluronic acid for enhancing wound healing J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-31 Zahra Sayyar, Gholam Reza Mahdavinia, Alireza Khataee
Montmorillonite (MMt) is extensively applied as an efficient drug-carrier in designing drug delivery systems (DDS) due to its high specific surface area to load drugs. Modification of MMt via iron (Fe) blending can thus be a desirable method to improve its biocompatibility. Herein, magnetic nano-carriers involving the magnetic MMt (mMMt) core surrounded by chitosan (Chito) as a biopolymer and hyaluronic
-
Engineered live bacteria as disease detection and diagnosis tools J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-24 Imen Tanniche, Bahareh Behkam
Sensitive and minimally invasive medical diagnostics are essential to the early detection of diseases, monitoring their progression and response to treatment. Engineered bacteria as live sensors are being developed as a new class of biosensors for sensitive, robust, noninvasive, and in situ detection of disease onset at low cost. Akin to microrobotic systems, a combination of simple genetic rules,
-
Novel ultra-stretchable and self-healing crosslinked poly (ethylene oxide)-cationic guar gum hydrogel J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-16 Sergio Alberto Bernal-Chávez, Sergio Alcalá-Alcalá, Zainab M. Almarhoon, Aknur Turgumbayeva, Eda Sönmez Gürer, Ma. De Los Dolores Campos-Echeverria, Hernán Cortés, Alejandra Romero-Montero, María Luisa Del Prado-Audelo, Javad Sharifi-Rad, Gerardo Leyva-Gómez
Hydrogels are three-dimensional structures with specific features that render them useful for biomedical applications, such as tissue engineering scaffolds, drug delivery systems, and wound dressings. In recent years, there has been a significant increase in the search for improved mechanical properties of hydrogels derived from natural products to extend their applications in various fields, and there
-
The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-05 Maryam Ehsasatvatan, Bahram Baghban Kohnehrouz
The high cost of fermentation, purification, cold storage and transportation, short shelf life, and sterile delivery methods of biopharmaceuticals, is a matter for producers and consumers as well. Since the FDA has now approved plant cells for large-scale, cost-effective biopharmaceutical production, the isolation and lyophilization of transplastomic chloroplasts can cover concerns about limitations
-
Biomolecular and cellular effects in skin wound healing: the association between ascorbic acid and hypoxia-induced factor J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-02 Maryam Ghahremani-Nasab, Azizeh Rahmani Del Bakhshayesh, Naeimeh Akbari-Gharalari, Ahmad Mehdipour
The skin serves as a barrier to protect the body from environmental microorganisms and is the largest tissue of the body and any damage must be quickly and effectively repaired. The fundamental purpose of dermal fibroblasts is to produce and secrete extracellular matrix, which is crucial for healing wounds. The production of collagen by dermal fibroblasts requires the cofactor ascorbic acid, a free
-
Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery J. Biol. Eng. (IF 5.6) Pub Date : 2023-10-02 Hajar Q. Alijani, Mehrdad Khatami, Masoud Torkzadeh-Mahani, Jan Michalička, Wu Wang, Di Wang, Abolfazl Heydari
Multicomponent nanoparticle systems are known for their varied properties and functions, and have shown potential as gene nanocarriers. This study aims to synthesize and characterize ternary nickel–cobalt-ferrite (NiCoFe2O4) nanoparticles with the potential to serve as gene nanocarriers for cancer/gene therapy. The biogenic nanocarriers were prepared using a simple and eco-friendly method following
-
Challenges of aortic valve tissue culture – maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system J. Biol. Eng. (IF 5.6) Pub Date : 2023-09-28 Claudia Dittfeld, Maximilian Winkelkotte, Anna Scheer, Emmely Voigt, Florian Schmieder, Stephan Behrens, Anett Jannasch, Klaus Matschke, Frank Sonntag, Sems-Malte Tugtekin
Calcific aortic valve disease (CAVD) causes an increasing health burden in the 21st century due to aging population. The complex pathophysiology remains to be understood to develop novel prevention and treatment strategies. Microphysiological systems (MPSs), also known as organ-on-chip or lab-on-a-chip systems, proved promising in bridging in vitro and in vivo approaches by applying integer AV tissue
-
Identification of histidine kinase inhibitors through screening of natural compounds to combat mastitis caused by Streptococcus agalactiae in dairy cattle J. Biol. Eng. (IF 5.6) Pub Date : 2023-09-26 Rajesh Kumar Pathak, Jun-Mo Kim
Mastitis poses a major threat to dairy farms globally; it results in reduced milk production, increased treatment costs, untimely compromised genetic potential, animal deaths, and economic losses. Streptococcus agalactiae is a highly virulent bacteria that cause mastitis. The administration of antibiotics for the treatment of this infection is not advised due to concerns about the emergence of antibiotic
-
Efficient targeting of HIF-1α mediated by YC-1 and PX-12 encapsulated niosomes: potential application in colon cancer therapy J. Biol. Eng. (IF 5.6) Pub Date : 2023-09-25 Azar Bakand, Sevil Vaghefi Moghaddam, Maryam Naseroleslami, Helder André, Neda Mousavi-Niri, Effat Alizadeh
A number of molecular biofactors have been documented in pathogenesis and poor prognosis of colorectal cancer (CRC). Among them, the Hypoxia-Inducible Factor (HIF-1a) is frequently reported to become over-expressed, and its targeting could restrict and control a variety of essential hallmarks of CRC. Niosomes are innovative drug delivery vehicles with the encapsulating capacity for co-loading both
-
Light regulation of secondary metabolism in fungi J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-31 Wenbin Yu, Rongqiang Pei, Yufei Zhang, Yayi Tu, Bin He
Fungi have evolved unique metabolic regulation mechanisms for adapting to the changing environments. One of the key features of fungal adaptation is the production of secondary metabolites (SMs), which are essential for survival and beneficial to the organism. Many of these SMs are produced in response to the environmental cues, such as light. In all fungal species studied, the Velvet complex transcription
-
Recent advances in 3D printing of biodegradable metals for orthopaedic applications J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-29 Wenqing Liang, Chao Zhou, Hongwei Zhang, Juqin Bai, Bo Jiang, Chanyi Jiang, Wenyi Ming, Hengjian Zhang, Hengguo Long, Xiaogang Huang, Jiayi Zhao
The use of biodegradable polymers for treating bone-related diseases has become a focal point in the field of biomedicine. Recent advancements in material technology have expanded the range of materials suitable for orthopaedic implants. Three-dimensional (3D) printing technology has become prevalent in healthcare, and while organ printing is still in its early stages and faces ethical and technical
-
Optimizing the fabrication of a 3D high-resolution implant for neural stimulation J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-24 Gal Shpun, Nairouz Farah, Yoav Chemla, Amos Markus, Tamar Azrad Leibovitch, Erel Lasnoy, Doron Gerber, Zeev Zalevsky, Yossi Mandel
Tissue-integrated micro-electronic devices for neural stimulation hold great potential in restoring the functionality of degenerated organs, specifically, retinal prostheses, which are aimed at vision restoration. The fabrication process of 3D polymer-metal devices with high resolution and a high aspect-ratio (AR) is very complex and faces many challenges that impair its functionality. Here we describe
-
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-18 Yikang Xu, Dacheng Ren
The increasing prevalence and severity of antimicrobial resistance (AMR) present a major challenge to our healthcare system. Rapid detection of AMR is essential for lifesaving under emergent conditions such as sepsis. The current gold standard phenotypic antibiotic susceptibility testing (AST) takes more than a day to obtain results. Genotypic ASTs are faster (hours) in detecting the presence of resistance
-
Cancer-on-chip: a 3D model for the study of the tumor microenvironment J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-17 Elisa Cauli, Michela Anna Polidoro, Simona Marzorati, Claudio Bernardi, Marco Rasponi, Ana Lleo
The approval of anticancer therapeutic strategies is still slowed down by the lack of models able to faithfully reproduce in vivo cancer physiology. On one hand, the conventional in vitro models fail to recapitulate the organ and tissue structures, the fluid flows, and the mechanical stimuli characterizing the human body compartments. On the other hand, in vivo animal models cannot reproduce the typical
-
Tissue poromechanical deformation effects on steam pop likelihood in 3-D radiofrequency cardiac ablation J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-07 Patcharaporn Wongchadakul, Ashim K. Datta, Phadungsak Rattanadecho
Radiofrequency Cardiac Ablation (RFCA) is a common procedure that heats cardiac tissue to destroy abnormal signal pathways to eliminate arrhythmias. The complex multiphysics phenomena during this procedure need to be better understood to improve both procedure and device design. A deformable poromechanical model of cardiac tissue was developed that coupled joule heating from the electrode, heat transfer
-
Fluid dynamic design for mitigating undesired cell effects and its application to testis cell response testing to endocrine disruptors J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-07 Seungjin Lee, Jinseop Ahn, Seok-Man Kim, Daehan Kim, Jiun Yeom, Jeongmok Kim, Joong Yull Park, Buom-Yong Ryu
Microfluidic devices have emerged as powerful tools for cell-based experiments, offering a controlled microenvironment that mimic the conditions within the body. Numerous cell experiment studies have successfully utilized microfluidic channels to achieve various new scientific discoveries. However, it has been often overlooked that undesired and unnoticed propagation of cellular molecules in such bio-microfluidic
-
Comparative transcriptome findings reveal the neuroinflammatory network and potential biomarkers to early detection of ischemic stroke J. Biol. Eng. (IF 5.6) Pub Date : 2023-08-02 Jiefeng Luo, Dingzhi Chen, Yujia Mei, Hepeng Li, Biyun Qin, Xiao Lin, Ting Fung Chan, Keng Po Lai, Deyan Kong
Ischemic stroke accounts for 70–80% of all stroke cases, leading to over two million people dying every year. Poor diagnosis and late detection are the major causes of the high death and disability rate. In the present study, we used the middle cerebral artery occlusion (MCAO) rat model and applied comparative transcriptomic analysis, followed by a systematic advanced bioinformatic analysis, including
-
Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-25 Natalia Carballo-Pedrares, Federica Ponti, Junquera Lopez-Seijas, Diego Miranda-Balbuena, Nina Bono, Gabriele Candiani, Ana Rey-Rico
In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell therapy for treating cartilage and bone injuries. Despite the tri-lineage multipotency, proliferative properties, and potent immunomodulatory effects of hMSCs, their clinical potential is hindered by donor variations, limiting their use in medical settings. To address this challenge, gene delivery technologies
-
An injectable, self-healing, electroconductive hydrogel loaded with neural stem cells and donepezil for enhancing local therapy effect of spinal cord injury J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-24 Tiemei Liu, Qiang Zhang, Hongru Li, Xiaoqian Cui, Zhiping Qi, Xiaoyu Yang
Spinal cord injury (SCI) is a serious injury with high mortality and disability rates, and there is no effective treatment at present. It has been reported that some treatments, such as drug intervention and stem cell transplantation have positive effects in promoting neurological recovery. Although those treatments are effective for nerve regeneration, many drawbacks, such as low stem cell survival
-
A novel computationally engineered collagenase reduces the force required for tooth extraction in an ex-situ porcine jaw model J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-17 Tamar Ansbacher, Ran Tohar, Adi Cohen, Orel Cohen, Shifra Levartovsky, Adi Arieli, Shlomo Matalon, Daniel Z. Bar, Maayan Gal, Evgeny Weinberg
The currently employed tooth extraction methods in dentistry involve mechanical disruption of the periodontal ligament fibers, leading to inevitable trauma to the bundle bone comprising the socket walls. In our previous work, we have shown that a recombinantly expressed truncated version of clostridial collagenase G (ColG) purified from Escherichia coli efficiently reduced the force needed for tooth
-
Study of the regulatory elements of the Ovalbumin gene promoter using CRISPR technology in chicken cells J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-17 Sara Yousefi Taemeh, Nima Dehdilani, Lena Goshayeshi, Sylvie Rival-Gervier, Jalil Mehrzad, Bertrand Pain, Hesam Dehghani
Hormone-dependent promoters are very efficient in transgene expression. Plasmid-based reporter assays have identified regulatory sequences of the Ovalbumin promoter that are involved in response to estrogen and have shown that the deletion of the steroid-dependent regulatory element (SDRE) and negative regulatory element (NRE) leads to a steroid-independent expression of a reporter. However, the functional
-
PLGA/BGP/Nef porous composite restrains osteoclasts by inhibiting the NF-κB pathway, enhances IGF-1-mediated osteogenic differentiation and promotes bone regeneration J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-17 Feng Wu, Zhenxu Wu, Zhijun Ye, Guoqing Niu, Zhiliang Ma, Peibiao Zhang
Novel bone substitutes are urgently needed in experimental research and clinical orthopaedic applications. There are many traditional Chinese medicines that have effects on bone repair. However, application of natural medicines in traditional Chinese medicine to bone tissue engineering and its mechanism were rarely reported. In this study, the osteogenic ability of bioactive glass particles (BGPs)
-
Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-11 Vitali V. Maldonado, Neel H. Patel, Emma E. Smith, C. Lowry Barnes, Michael P. Gustafson, Raj R. Rao, Rebekah M. Samsonraj
Mesenchymal stem/stromal cells (MSCs) have been carefully examined to have tremendous potential in regenerative medicine. With their immunomodulatory and regenerative properties, MSCs have numerous applications within the clinical sector. MSCs have the properties of multilineage differentiation, paracrine signaling, and can be isolated from various tissues, which makes them a key candidate for applications
-
Dual growth factor-modified microspheres nesting human-derived umbilical cord mesenchymal stem cells for bone regeneration J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-10 Wenzhi Song, Lanlan Zhao, Yuqi Gao, Chunyu Han, Shengrui Gao, Min Guo, Jianfei Bai, Liqiang Wang, Wanzhong Yin, Feng Wu, Peibiao Zhang
Modular tissue engineering (MTE) is a novel “bottom-up” approach that aims to mimic complex tissue microstructural features. The constructed micromodules are assembled into engineered biological tissues with repetitive functional microunits and form cellular networks. This is emerging as a promising strategy for reconstruction of biological tissue. Herein, we constructed a micromodule for MTE and developed
-
Sandwich-like electro-conductive polyurethane-based gelatin/soybean oil nanofibrous scaffolds with a targeted release of simvastatin for cardiac tissue engineering J. Biol. Eng. (IF 5.6) Pub Date : 2023-07-06 Solmaz Saghebasl, Abbas Nobakht, Hesam Saghebasl, Sanya Hayati, Ozra Naturi, Reza Rahbarghazi
Cardiac tissue engineering (CTE) is a promising way for the restoration of injured cardiac tissue in the healthcare system. The development of biodegradable scaffolds with appropriate chemical, electrical, mechanical, and biological properties is an unmet need for the success of CTE. Electrospinning is a versatile technique that has shown potential applications in CTE. Herein, four different types
-
Breast cancer brain metastasis: from etiology to state-of-the-art modeling J. Biol. Eng. (IF 5.6) Pub Date : 2023-06-29 Mohammad Kamalabadi Farahani, Maliheh Gharibshahian, Alireza Rezvani, Ahmad Vaez
Currently, breast carcinoma is the most common form of malignancy and the main cause of cancer mortality in women worldwide. The metastasis of cancer cells from the primary tumor site to other organs in the body, notably the lungs, bones, brain, and liver, is what causes breast cancer to ultimately be fatal. Brain metastases occur in as many as 30% of patients with advanced breast cancer, and the 1-year
-
A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance J. Biol. Eng. (IF 5.6) Pub Date : 2023-06-20 Dafna Rivka Levenberg, Eli Varon, Ganit Indech, Tal Ben Uliel, Lidor Geri, Amos Sharoni, Orit Shefi
The ability to control neuronal mobility and organization is of great importance in developing neuronal interfaces and novel therapeutic approaches. An emerging promising method is the manipulation of neuronal cells from afar via magnetic forces. Nevertheless, using magnetic iron oxide nanoparticles as internal actuators may lead to biotoxicity, adverse influence on intracellular processes, and thus