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  • Including Digital Sequence Data in the Nagoya Protocol Can Promote Data Sharing
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-10
    Jon Ambler; Alpha Ahmadou Diallo; Peter K. Dearden; Phil Wilcox; Maui Hudson; Nicki Tiffin

    The Nagoya Protocol (NP), a legal framework under the Convention on Biological Diversity (CBD), formalises fair and equitable sharing of benefits arising from biological diversity. It encompasses biological samples and associated indigenous knowledge, with equitable return of benefits to those providing samples. Recent proposals that the use of digital sequence information (DSI) derived from samples

  • Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-09
    Sara Amorim; Celso A. Reis; Rui L. Reis; Ricardo A. Pires

    Hyaluronan (HA) is a critical element of the extracellular matrix (ECM). The regulated synthesis and degradation of HA modulates the ECM chemical and physical properties that, in turn, influence cellular behavior. HA triggers signaling pathways associated with the adhesion, proliferation, migration, and differentiation of cells, mediated by its interaction with specific cellular receptors or by tuning

  • Reverse Transcriptase: From Transcriptomics to Genome Editing
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-08
    Samara Martín-Alonso; Estrella Frutos-Beltrán; Luis Menéndez-Arias

    Reverse transcriptases (RTs) are enzymes that can generate a complementary strand of DNA (cDNA) from RNA. Coupled with PCR, RTs have been widely used to detect RNAs and to clone expressed genes. Classical retroviral RTs have been improved by protein engineering. These enzymes and newly characterized RTs are key elements in the development of next-generation sequencing techniques that are now being

  • Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-07
    Chunching Li; Liliang Ouyang; James P.K. Armstrong; Molly M. Stevens

    Natural tissues and organs exhibit an array of spatial gradients, from the polarized neural tube during embryonic development to the osteochondral interface present at articulating joints. The strong structure–function relationships in these heterogeneous tissues have sparked intensive research into the development of methods that can replicate physiological gradients in engineered tissues. In this

  • Electron Storage in Electroactive Biofilms
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-06
    A. ter Heijne; M.A. Pereira; J. Pereira; T. Sleutels

    Microbial electrochemical technologies (METs) are promising for sustainable applications. Recently, electron storage during intermittent operation of electroactive biofilms (EABs) has been shown to play an important role in power output and electron efficiencies. Insights into electron storage mechanisms, and the conditions under which these occur, are essential to improve microbial electrochemical

  • New Twists in Detecting mRNA Modification Dynamics
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-01
    Ina Anreiter; Quoseena Mir; Jared T. Simpson; Sarath C. Janga; Matthias Soller

    Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and

  • Emergence of the Stem Cell Secretome in Regenerative Engineering
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-07-01
    Leila Daneshmandi; Shiv Shah; Tahereh Jafari; Maumita Bhattacharjee; Deandra Momah; Nikoo Saveh-Shemshaki; Kevin W-H. Lo; Cato T. Laurencin

    The secretome is defined as the set of molecules and biological factors that are secreted by cells into the extracellular space. In the past decade, secretome-based therapies have emerged as a promising approach to overcome the limitations associated with cell-based therapies for tissue and organ regeneration. Considering the growing number of recent publications related to secretome-based therapies

  • The Formidable Challenge of Controlling High Mannose-Type N-Glycans in Therapeutic mAbs
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-29
    Renato Mastrangeli; Maria Concetta Audino; Wolf Palinsky; Hervé Broly; Horst Bierau

    The clinical efficacy and safety of therapeutic monoclonal antibodies (mAbs) are significantly affected by their Fc-glycosylation profile. High mannose-type N-glycans (HM) affect efficacy (in terms of antibody-dependent cell cytotoxicity), pharmacokinetics, and stability. While in endogenous IgGs the HM levels are very low, they are significantly higher in marketed therapeutic mAbs. In order to meet

  • Microvascular Fragments: More Than Just Natural Vascularization Units
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-24
    Matthias W. Laschke; Thomas Später; Michael D. Menger

    Adipose tissue–derived microvascular fragments serve as natural vascularization units in angiogenesis research and tissue engineering due to their ability to rapidly reassemble into microvascular networks. Recent studies indicate that they exhibit additional unique properties that may be beneficial for a wide range of future biomedical applications. Their angiogenic activity can be increased during

  • Synthetic Biological Circuits within an Orthogonal Central Dogma
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-22
    Alan Costello; Ahmed H. Badran

    Synthetic biology strives to reliably control cellular behavior, typically in the form of user-designed interactions of biological components to produce a predetermined output. Engineered circuit components are frequently derived from natural sources and are therefore often hampered by inadvertent interactions with host machinery, most notably within the host central dogma. Reliable and predictable

  • Technologies and Standardization in Research on Extracellular Vesicles.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-18
    Srujan Gandham,Xianyi Su,Jacqueline Wood,Angela L Nocera,Sarath Chandra Alli,Lara Milane,Alan Zimmerman,Mansoor Amiji,Alexander R Ivanov

    Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component of a variety of physiological and pathological processes. Potential applications of EVs in novel diagnostic and therapeutic strategies

  • Allergenicity Assessment of Novel Food Proteins: What Should Be Improved?
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-13
    Antonio Fernandez,E N Clare Mills,Frits Koning,F Javier Moreno

    Allergenicity prediction is one of the most challenging aspects in the safety assessment of foods derived from either biotechnology or novel food proteins. Here we present a bottom-up strategy that defines a priori the specific risk assessment (RA) needs based on a database appropriately built for such purposes.

  • Working hard or hardly working? Regulatory bottlenecks in developing a COVID-19 vaccine
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-13
    Lisette Pregelj; Damian C. Hine; Maria G. Oyola-Lozada; Trent P. Munro

    Abstract Vaccine solutions rarely reach the public until after an outbreak abates; an Ebola vaccine was approved 5-years after peak outbreak and SARS, MERS, and Zika vaccines are still in clinical development. Despite massive leaps forward in rapid science, other regulatory bottlenecks are hamstringing the global effort for pandemic vaccines.

  • Sustainable Transformation of Land-Based Economic Development in the Era of Digital Revolution.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-13
    Chun Sheng Goh,Amanda Ahl,Wing Thye Woo

    Biotechnology will play a key role in transforming current land-use systems alongside the digital revolution by using five strategies: enhancing productivity at the farm or plantation level, replenishing degraded land, enabling landscape management for resilience, upgrading and diversifying downstream activities, and creating new value propositions.

  • Towards a Digital Bioprocess Replica: Computational Approaches in Biopharmaceutical Development and Manufacturing.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-06
    Jens Smiatek,Alexander Jung,Erich Bluhmki

    Quantitative unit operation models for the optimization and refinement of modern late-stage biopharmaceutical drug manufacturing processes have recently attracted increasing attention. The supplementary benefits of these models include increased process robustness and control in combination with a more stringent design of the bioprocess due to a reduced number of exploratory experiments. In addition

  • Uncovering an Organ's Molecular Architecture at Single-Cell Resolution by Spatially Resolved Transcriptomics.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-03
    Jie Liao,Xiaoyan Lu,Xin Shao,Ling Zhu,Xiaohui Fan

    Revealing fine-scale cellular heterogeneity among spatial context and the functional and structural foundations of tissue architecture is fundamental within biological research and pharmacology. Unlike traditional approaches involving single molecules or bulk omics, cutting-edge, spatially resolved transcriptomics techniques offer near-single-cell or even subcellular resolution within tissues. Massive

  • Hacking Human Beings with Machine Biology to Increase Lifespan.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-01
    Gorka Orive,Nayere Taebnia,Itsasne Erezuma,Thomas L Andresen,Alireza Dolatshahi-Pirouz

    Imagine a world where machines can program cells to deliver therapeutics in a remote-controlled, time-specific, and targeted manner. Or, what if physicians could collect data continuously to establish intimate links between therapy and disease progression? Such machine biology systems could empower physicians beyond imagination and give rise to personalized treatments.

  • Non-Hydrolyzable Plastics - An Interdisciplinary Look at Plastic Bio-Oxidation.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-30
    Hedda Inderthal,Siew Leng Tai,Susan T L Harrison

    Enzymatic plastic conversion has emerged recently as a potential adjunct and alternative to conventional plastic waste management technology. Publicity over progress in the enzymatic degradation of polyesters largely neglects that the majority of commercial plastics, including polyethylene, polypropylene, polystyrene and polyvinyl chloride, are still not biodegradable. Details about the mechanisms

  • RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-27
    Saba Safdar,Jeroen Lammertyn,Dragana Spasic

    Nucleic acid enzymes (NAzymes) are nucleic acid molecules with catalytic activity. A subset, the RNA-cleaving NAzyme, is characterized by its substrate of choice: an RNA unit. These enzymes have been used for diverse applications, including biosensor development, akin to their protein counterparts. Owing to their function as both biorecognition elements and signal generators, robust bioassays based

  • Multitechnology Biofabrication: A New Approach for the Manufacturing of Functional Tissue Structures?
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-25
    Miguel Castilho,Mylène de Ruijter,Stephen Beirne,Claire C Villette,Keita Ito,Gordon G Wallace,Jos Malda

    Most available 3D biofabrication technologies rely on single-component deposition methods, such as inkjet, extrusion, or light-assisted printing. It is unlikely that any of these technologies used individually would be able to replicate the complexity and functionality of living tissues. Recently, new biofabrication approaches have emerged that integrate multiple manufacturing technologies into a single

  • Rethinking Cancer Immunotherapy by Embracing and Engineering Complexity.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-25
    Matthew H W Chin,Eileen Gentleman,Marc-Olivier Coppens,Richard M Day

    The meteoric rise of cancer immunotherapy in the past decade has led to promising treatments for a number of hard-to-treat malignancies. In particular, adoptive T cell therapy has recently reached a major milestone with two products approved by the US FDA. However, the inherent complexity of cell-based immunotherapies means that their manufacturing time, cost, and controllability limit their effectiveness

  • RNAi: Applications in Vertebrate Pest Management.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-25
    Katherine E Horak

    Sequence-directed inhibition of protein synthesis by RNAi has potential as a means to control pest wildlife. Species specific by design, RNAi reduces impacts on nontarget species and the environment. Additional research advancing the field of RNAi-based management of vertebrate pest wildlife is timely.

  • Decellularized Scaffolds for Intervertebral Disc Regeneration.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-25
    Morena Fiordalisi,Ana João Silva,Mário Barbosa,Raquel Gonçalves,Joana Caldeira

    In the last decade, intervertebral disc (IVD) decellularization has gained significant attention for tissue regenerative purposes as a successful therapeutic alternative for low back pain (LBP). We discuss the recent advances in IVD decellularization, repopulation, and sterilization procedures, highlighting the major challenges that need to be addressed for clinical translation.

  • Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-22
    Inessa Arif,Maria Batool,Peer M Schenk

    Plant-associated microbiomes can boost plant growth or control pathogens. Altering the microbiome by inoculation with a consortium of plant growth-promoting rhizobacteria (PGPR) can enhance plant development and mitigate against pathogens as well as abiotic stresses. Manipulating the plant holobiont by microbiome engineering is an emerging biotechnological strategy to improve crop yields and resilience

  • Putting Photosystem I to Work: Truly Green Energy.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-21
    Alexandra H Teodor,Barry D Bruce

    Meeting growing energy demands sustainably is one of the greatest challenges facing the world. The sun strikes the Earth with sufficient energy in 1.5 h to meet annual world energy demands, likely making solar energy conversion part of future sustainable energy production plans. Photosynthetic organisms have been evolving solar energy utilization strategies for nearly 3.5 billion years, making reaction

  • Microfluidics in Single-Cell Virology: Technologies and Applications.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-16
    Wu Liu,Hongzhang He,Si-Yang Zheng

    Microfluidics has proven to be a powerful tool for probing biology at the single-cell level. However, it is only in the past 5 years that single-cell microfluidics has been used in the field of virology. An array of strategies based on microwells, microvalves, and droplets is now available for tracking viral infection dynamics, identifying cell subpopulations with particular phenotypes, as well as

  • Harnessing the Power of Enzymes for Tailoring and Valorizing Lignin.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-15
    Renate Weiss,Georg M Guebitz,Alessandro Pellis,Gibson S Nyanhongo

    Lignin, a structural component of lignocellulosic plants, is an alternative raw material with enormous potential to replace diminishing fossil-based resources for the sustainable production of many chemicals and materials. Unfortunately, lignin's heterogeneity, low reactivity, and strong intra- and intermolecular hydrogen interactions and modifications introduced during the pulping process present

  • Combining Environmental and Economic Performance for Bioprocess Optimization.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-15
    Ólafur Ögmundarson,Sumesh Sukumara,Markus J Herrgård,Peter Fantke

    Biochemical production faces economic and environmental challenges that need to be overcome to enable a viable and sustainable bioeconomy. We propose an assessment framework that consistently combines environmental and economic indicators to support optimized biochemical production at early development stages. We define internally consistent system boundaries and a comprehensive set of quantitative

  • The Business of Cultured Meat.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-06-01
    Deepak Choudhury,Ting Wei Tseng,Elliot Swartz

    Cultured meats (CMs) are produced by in vitro culture of animal cells. Since the first CM burger patty was created in 2013, many companies have been founded to commercialize CM products. We discuss the meat focus and geographical spread of CM companies, the funding landscape, and challenges for commercialization.

  • Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D in Vitro Tumor Models.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-13
    Luís P Ferreira,Vítor M Gaspar,João F Mano

    Recent advances in the extraction and purification of decellularized extracellular matrix (dECM) obtained from healthy or malignant tissues open new avenues for engineering physiomimetic 3D in vitro tumor models, which closely recapitulate key biomolecular hallmarks and the dynamic cancer cell-ECM interactions in the tumor microenvironment. We review current and upcoming methodologies for chemical

  • Creating Bioethics Distance Learning Through Virtual Reality.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-11
    Antoine L Harfouche,Farid Nakhle

    Bioethics education is a central element in the biotechnology curriculum. Re-imagining distance learning, virtual reality (VR) is taking student involvement to the next level of interaction, offering a real classroom experience and a new way to gain ethical reasoning skills. Here, we explore a new paradigm for bioethics education that involves VR.

  • Minicircle DNA: The Future for DNA-Based Vectors?
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-11
    Ana Margarida Almeida,João António Queiroz,Fani Sousa,Ângela Sousa

    Minicircle DNA (mcDNA) is a smaller and safer version of non-viral DNA vectors that results from a cutting-edge in vivo recombination process to excise prokaryotic sequences from plasmid DNA (pDNA). Considering the molecule's potential and increasing interest as a non-viral DNA-based therapeutic, biomanufacturing methodologies need to be improved, especially in downstream processing.

  • Microbiome-Inspired Green Infrastructure: A Toolkit for Multidisciplinary Landscape Design.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-10
    Harry Watkins,Jake M Robinson,Martin F Breed,Brenda Parker,Philip Weinstein

    Incorporating recent advances in environmental microbiome research and policy is a major challenge for urban design. We set out a framework for managing construction projects so that multidisciplinary teams of researchers and practitioners can explicitly consider environmental microbiota in design and construction contexts, thereby increasing ecosystem functionality and public health.

  • Genetic Safeguards for Safety-by-design: So Close Yet So Far.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-10
    Enrique Asin-Garcia,Amalia Kallergi,Laurens Landeweerd,Vitor A P Martins Dos Santos

    Safety-by-design (SbD) is paramount for risk management in synthetic biology, with genetic safeguards being a key technology for its implementation. While attractive in theory, the integration of genetic safeguards into SbD strategies is rarely exercised in practice, despite technological advances. Here we question why and what might be done about it.

  • Engineering Microbes for Remediation of Oil Sands Tailings.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-08
    Parisa Chegounian,Hisham Zerriffi,Vikramaditya G Yadav

    Synthetic biology and adaptive laboratory evolution are key tools for developing biotechnology platforms for the remediation of oil sands tailings. However, field deployment and subsequent regulation of engineered and/or evolved strains is rife with uncertainties and risks. Here, we detail an innovation strategy to derisk and deploy engineered bioremediation platforms.

  • Challenges and Opportunities for the Biotechnology Research Community during the Coronavirus Pandemic.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-07
    Sergio Rosales-Mendoza,Mauricio Comas-García,Schuyler S Korban

    The coronavirus disease 2019 (COVID-19) pandemic has presented some significant challenges to the scientific community. However, this has also offered opportunities for the pursuit of new scientific activities, and in particular for the field of biotechnology.

  • Sustainable Seaweed Biotechnology Solutions for Carbon Capture, Composition, and Deconstruction.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-06
    Lieve M L Laurens,Madeline Lane,Robert S Nelson

    Seaweeds or macroalgae are attractive candidates for carbon capture, while also supplying a sustainable photosynthetic bioenergy feedstock, thanks to their cultivation potential in offshore marine farms. Seaweed cultivation requires minimal external nutrient requirements and allows for year-round production of biomass. Despite this potential, there remain significant challenges associated with realizing

  • The Biofilms Structural Database.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-05-05
    Rita P Magalhães,Tatiana F Vieira,Henrique S Fernandes,André Melo,Manuel Simões,Sérgio F Sousa

    The Biofilms Structural Database (BSD) is a collection of structural, mutagenesis, kinetics, and inhibition data to understand the processes involved in biofilm formation. Presently, it includes curated information on 425 structures of proteins and enzymes involved in biofilm formation and development for 42 different bacteria. It is available at www.biofilms.biosim.pt.

  • Novel Strategies and Platforms for Industrial Isoprenoid Engineering.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-29
    Georgios Daletos,Constantinos Katsimpouras,Gregory Stephanopoulos

    Metabolic engineering has emerged as an important tool for reconstructing heterologous isoprenoid metabolic pathways in microbial hosts. Here, we provide an overview of promising engineering strategies that have proven to be successful for the high-yield production of isoprenoids. Besides ‘conventional’ approaches, such as the ‘push–pull’ and protein engineering to optimize the isoprenoid flux and

  • Genetic Biosensor Design for Natural Product Biosynthesis in Microorganisms.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-28
    Gazi Sakir Hossain,Mukesh Saini,Ryoma Miyake,Hua Ling,Matthew Wook Chang

    Low yield and low titer of natural products are common issues in natural product biosynthesis through microbial cell factories. One effective way to resolve such bottlenecks is to design genetic biosensors to monitor and regulate the biosynthesis of target natural products. In this review, we evaluate the most recent advances in the design of genetic biosensors for natural product biosynthesis in microorganisms

  • Waste or Gold? Bioelectrochemical Resource Recovery in Source-Separated Urine.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-25
    Safoora Nazari,Ali A Zinatizadeh,Mahsa Mirghorayshi,Mark C M van Loosdrecht

    In recent years, source-separated human urine has been highlighted as an effective resource for energy and nutrient recovery. However, even though several technologies exist for resource recovery, they have not been widely implemented. Among these technologies, bioelectrochemical systems (BESs) hold promise as technically and economically interesting alternatives for sustainable resource recovery from

  • Biotechnological Potential for Microplastic Waste.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-21
    Abhishek Kumar Awasthi,Quanyin Tan,Jinhui Li

    Plastic has become a critical material in the modern world due to its low cost and easy production. However, inadequate management and open disposal make plastic a major obstacle to achieving the UN Sustainable Development Goals. This article emphasizes the role of biotechnology in addressing the issue of microplastic waste.

  • Fifty Shades of Blue: How Blue Biotechnology is Shaping the Bioeconomy.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-20
    Helena Vieira,Miguel Costa Leal,Ricardo Calado

    The bioeconomy is a new paradigm for the sustainable development of society. Novel uses of blue bioresources and biotechnology solutions, co-created with value chain stakeholders, accelerate the bioeconomy, foster innovation, and promote novel circular business models. Bottom-up approaches sharing visions, needs, and expertise are key to the successful implementation of bioeconomy initiatives.

  • Clicking into Place: Interfacing Terminal Alkyne Biosynthesis with Polyketide Synthases.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-21
    Munro Passmore,Matthew Jenner

    Engineering polyketide biosynthesis to enable the production of diverse chemical structures is a major challenge at present. Utilising an established biosynthetic cassette for terminal alkyne production, Porterfield et al. applied docking domain and site-directed mutagenesis approaches to interface these enzymes with modular polyketide synthase (PKS) enzymes, yielding products with a bio-orthogonal

  • Expanding the Reach of Recombineering to Environmental Bacteria.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-17
    José Manuel Borrero-de Acuña,Ignacio Poblete-Castro

    Broadening the application of recombineering technologies in biotechnologically important bacteria poses significant challenges. Aparicio et al. present a vital breakthrough for efficient single-stranded recombineering by utilizing a thermoinducible system in Pseudomonas putida. This offers a simple genome-editing tool towards creating superior biocatalysts for the synthesis of chemicals and for bioremediation

  • Cold Plasma, a New Hope in the Field of Virus Inactivation.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-17
    Arijana Filipić,Ion Gutierrez-Aguirre,Gregor Primc,Miran Mozetič,David Dobnik

    Viruses can infect all cell-based organisms, from bacteria to humans, animals, and plants. They are responsible for numerous cases of hospitalization, many deaths, and widespread crop destruction, all of which result in an enormous medical, economical, and biological burden. Each of the currently used decontamination methods has important drawbacks. Cold plasma (CP) has entered this field as a novel

  • Using Microbial Aggregates to Entrap Aqueous Phosphorus.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-17
    Ying Xu,Yonghong Wu,Sofia Esquivel-Elizondo,Jan Dolfing,Bruce E Rittmann

    The increasing use and associated loss of phosphorus to the environment pose risks to aquatic ecosystems. Technology for phosphorus removal based on microbial aggregates is a natural, ecologically widespread, and sustainable reclamation strategy. Two main processes dominate phosphorus removal by microbial aggregates: extra- and intra-cellular entrapment. Extracellular phosphorus entrapment relies on

  • Material-Microbe Interfaces for Solar-Driven CO2 Bioelectrosynthesis.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-15
    Prakash C Sahoo,Deepak Pant,Manoj Kumar,S K Puri,S S V Ramakumar

    Sustainable production of solar-based chemicals is possible by mimicking the natural photosynthetic mechanism. To realize the full potential of solar-to-chemical production, the artificial means of photosynthesis and the biological approach should complement each other. The recently developed hybrid microbe-metal interface combines an inorganic, semiconducting light-harvester material with efficient

  • 更新日期:2020-04-14
  • Plant Aromatic Prenyltransferases: Tools for Microbial Cell Factories.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-13
    Wouter J C de Bruijn,Mark Levisson,Jules Beekwilder,Willem J H van Berkel,Jean-Paul Vincken

    In plants, prenylation of aromatic compounds, such as (iso)flavonoids and stilbenoids, by membrane-bound prenyltransferases (PTs), is an essential step in the biosynthesis of many bioactive compounds. Prenylated aromatic compounds have various health-beneficial properties that are interesting for industrial applications, but their exploitation is limited due to their low abundance in nature. Harnessing

  • New Cognitive Neurotechnology Facilitates Studies of Cortical–Subcortical Interactions
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-08
    Byoung-Kyong Min; Matti S. Hämäläinen; Dimitrios Pantazis

    Most of the studies employing neuroimaging have focused on cortical and subcortical signals individually to obtain neurophysiological signatures of cognitive functions. However, understanding the dynamic communication between the cortex and subcortical structures is essential for unraveling the neural correlates of cognition. In this quest, magnetoencephalography (MEG) and electroencephalography (EEG)

  • Bioprocess Technologies that Preserve the Quality of iPSCs
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-05
    Ashli Polanco; Bingyu Kuang; Seongkyu Yoon

    Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods

  • Regulation of Metabolic Homeostasis in Cell Culture Bioprocesses
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-02
    Conor M. O'Brien; Bhanu Chandra Mulukutla; Douglas G. Mashek; Wei-Shou Hu

    Mammalian cells are the main tool for the production of therapeutic proteins, viruses for gene therapy, and cells for cell therapy. In production processes cell metabolism is the main driver that causes changes in the growth environment and affects productivity and product quality. Of all nutrients, glucose has the most prominent impact on bioprocesses. We summarize recent findings on the regulation

  • On-Site Therapeutic Drug Monitoring
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-02
    H. Ceren Ates; Jason A. Roberts; Jeffrey Lipman; Anthony E.G. Cass; Gerald A. Urban; Can Dincer

    Recent technological advances have stimulated efforts to bring personalized medicine into practice. Yet, traditional application fields like therapeutic drug monitoring (TDM) have remained rather under-appreciated. Owing to clear dose-response relationships, TDM could improve patient outcomes and reduce healthcare costs. While chromatography-based routine practices are restricted due to high costs

  • Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-04-01
    Emmanuel A. Margolin; Richard Strasser; Ros Chapman; Anna-Lise Williamson; Edward P. Rybicki; Ann E. Meyers

    Production of biologics in plants, or plant molecular pharming, is a promising protein expression technology that is receiving increasing attention from the pharmaceutical industry. Previously, low expression yields of recombinant proteins and the realization that certain post-translational modifications (PTMs) may not occur optimally limited the widespread acceptance of the technology. However, molecular

  • Integrative Methods and Practical Challenges for Single-Cell Multi-omics
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-03-26
    Anjun Ma; Adam McDermaid; Jennifer Xu; Yuzhou Chang; Qin Ma

    Fast-developing single-cell multimodal omics (scMulti-omics) technologies enable the measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA expression, protein abundance, gene perturbation, and spatial information, from the same cell. scMulti-omics can comprehensively explore and identify cell characteristics, while also presenting challenges to the development of

  • Microalgal Torrefaction for Solid Biofuel Production
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-03-23
    Shih-Hsin Ho; Congyu Zhang; Fei Tao; Chaofan Zhang; Wei-Hsin Chen

    Microalgae are recognized as promising feedstocks for biofuel production and have been intensively studied. However, a systematic investigation of the relationship between cellular components and fuel properties in microalgae is currently absent. This review introduces recent advances in microalgal torrefaction and critically evaluates the properties of torrefied products from the viewpoint of molecular

  • Functional Applications of Nucleic Acid–Protein Hybrid Nanostructures
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-03-19
    Joshua B. McCluskey; Douglas S. Clark; Dominic J. Glover

    Combining the diverse chemical functionality of proteins with the predictable structural assembly of nucleic acids has enabled the creation of hybrid nanostructures for a range of biotechnology applications. Through the attachment of proteins onto or within nucleic acid nanostructures, materials with dynamic capabilities can be created that include switchable enzyme activity, targeted drug delivery

  • Multidimensional Metabolic Engineering for Constructing Efficient Cell Factories.
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-03-19
    Jianhui Liu,Jin Hou

    Microbial production of medium-chain fatty acids (MCFAs) is challenging, as they are rare metabolites and toxic to cells. Recently, Zhu et al. performed multidimensional engineering in Saccharomyces cerevisiae, including engineering fatty acid synthases and improving cellular tolerance to MCFAs, to build an efficient MCFA cell factory.

  • Burkholderia: An Untapped but Promising Bacterial Genus for the Conversion of Aromatic Compounds
    Trends Biotechnol. (IF 14.343) Pub Date : 2020-03-18
    Raj Morya; Davinia Salvachúa; Indu Shekhar Thakur

    Burkholderia, a bacterial genus comprising more than 120 species, is typically reported to inhabit soil and water environments. These Gram-negative bacteria harbor a variety of aromatic catabolic pathways and are thus potential organisms for bioremediation of sites contaminated with aromatic pollutants. However, there are still substantial gaps in our knowledge of these catabolic processes that must

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