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  • Bioprocess Technologies that Preserve the Quality of iPSCs
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-04-05
    Ashli Polanco; Bingyu Kuang; Seongkyu Yoon

    Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods

  • Regulation of Metabolic Homeostasis in Cell Culture Bioprocesses
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-04-02
    Conor M. O'Brien; Bhanu Chandra Mulukutla; Douglas G. Mashek; Wei-Shou Hu

    Mammalian cells are the main tool for the production of therapeutic proteins, viruses for gene therapy, and cells for cell therapy. In production processes cell metabolism is the main driver that causes changes in the growth environment and affects productivity and product quality. Of all nutrients, glucose has the most prominent impact on bioprocesses. We summarize recent findings on the regulation

  • On-Site Therapeutic Drug Monitoring
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-04-02
    H. Ceren Ates; Jason A. Roberts; Jeffrey Lipman; Anthony E.G. Cass; Gerald A. Urban; Can Dincer

    Recent technological advances have stimulated efforts to bring personalized medicine into practice. Yet, traditional application fields like therapeutic drug monitoring (TDM) have remained rather under-appreciated. Owing to clear dose-response relationships, TDM could improve patient outcomes and reduce healthcare costs. While chromatography-based routine practices are restricted due to high costs

  • Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-04-01
    Emmanuel A. Margolin; Richard Strasser; Ros Chapman; Anna-Lise Williamson; Edward P. Rybicki; Ann E. Meyers

    Production of biologics in plants, or plant molecular pharming, is a promising protein expression technology that is receiving increasing attention from the pharmaceutical industry. Previously, low expression yields of recombinant proteins and the realization that certain post-translational modifications (PTMs) may not occur optimally limited the widespread acceptance of the technology. However, molecular

  • Integrative Methods and Practical Challenges for Single-Cell Multi-omics
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-26
    Anjun Ma; Adam McDermaid; Jennifer Xu; Yuzhou Chang; Qin Ma

    Fast-developing single-cell multimodal omics (scMulti-omics) technologies enable the measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA expression, protein abundance, gene perturbation, and spatial information, from the same cell. scMulti-omics can comprehensively explore and identify cell characteristics, while also presenting challenges to the development of

  • Microalgal Torrefaction for Solid Biofuel Production
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-23
    Shih-Hsin Ho; Congyu Zhang; Fei Tao; Chaofan Zhang; Wei-Hsin Chen

    Microalgae are recognized as promising feedstocks for biofuel production and have been intensively studied. However, a systematic investigation of the relationship between cellular components and fuel properties in microalgae is currently absent. This review introduces recent advances in microalgal torrefaction and critically evaluates the properties of torrefied products from the viewpoint of molecular

  • Functional Applications of Nucleic Acid–Protein Hybrid Nanostructures
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-19
    Joshua B. McCluskey; Douglas S. Clark; Dominic J. Glover

    Combining the diverse chemical functionality of proteins with the predictable structural assembly of nucleic acids has enabled the creation of hybrid nanostructures for a range of biotechnology applications. Through the attachment of proteins onto or within nucleic acid nanostructures, materials with dynamic capabilities can be created that include switchable enzyme activity, targeted drug delivery

  • Multidimensional Metabolic Engineering for Constructing Efficient Cell Factories
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-19
    Jianhui Liu; Jin Hou

    Microbial production of medium-chain fatty acids (MCFAs) is challenging, as they are rare metabolites and toxic to cells. Recently, Zhu et al. performed multidimensional engineering in Saccharomyces cerevisiae, including engineering fatty acid synthases and improving cellular tolerance to MCFAs, to build an efficient MCFA cell factory.

  • Burkholderia: An Untapped but Promising Bacterial Genus for the Conversion of Aromatic Compounds
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-18
    Raj Morya; Davinia Salvachúa; Indu Shekhar Thakur

    Burkholderia, a bacterial genus comprising more than 120 species, is typically reported to inhabit soil and water environments. These Gram-negative bacteria harbor a variety of aromatic catabolic pathways and are thus potential organisms for bioremediation of sites contaminated with aromatic pollutants. However, there are still substantial gaps in our knowledge of these catabolic processes that must

  • Cybersecurity in Science and Medicine: Threats and Challenges
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-18
    Frank Luh; Yun Yen

    Technology offers opportunities to revolutionize medicine and research but can threaten privacy and patient confidentiality. As the scale of patient data explodes, the safety and integrity of medical information are increasingly at stake. We highlight security and privacy issues associated with genomic research, medical devices, and wearable technology.

  • Obscure yet Promising Oleaginous Yeasts for Fuel and Chemical Production
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-12
    Rachapudi Venkata Sreeharsha; S. Venkata Mohan

    The rapid depletion of petroleum-based fossil fuels has led to the development of alternative fuel and energy solutions from renewable resources. Lipid-derived oleochemicals and sugar-derived bioethanol were initially produced from plant feedstocks, but in the interest of agricultural land conservation and food security, the focus has now shifted toward so-called ‘third-generation feedstocks’, of which

  • 100 Years Later, What Is New in Glycerol Bioproduction?
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-12
    Marta V. Semkiv; Justyna Ruchala; Kostyantyn V. Dmytruk; Andriy A. Sibirny

    Industrial production of glycerol by yeast, which began during WWI in the so-called Neuberg fermentation, was the first example of metabolic engineering. However, this process, based on bisulfite addition to fermentation liquid, has many drawbacks and was replaced by other methods of glycerol production. Osmotolerant yeasts and other microorganisms that do not require addition of bisulfite to steer

  • Engineering Heart Morphogenesis
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-05
    Christian J. Mandrycky; Nisa P. Williams; Ivan Batalov; Danny El-Nachef; Bernadette S. de Bakker; Jennifer Davis; Deok-Ho Kim; Cole A. DeForest; Ying Zheng; Kelly R. Stevens; Nathan J. Sniadecki

    Recent advances in stem cell biology and tissue engineering have laid the groundwork for building complex tissues in a dish. We propose that these technologies are ready for a new challenge: recapitulating cardiac morphogenesis in vitro. In development, the heart transforms from a simple linear tube to a four-chambered organ through a complex process called looping. Here, we re-examine heart tube looping

  • Microbial Lipid Biotechnology to Produce Polyunsaturated Fatty Acids
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-02
    Xiao-Jun Ji; Rodrigo Ledesma-Amaro

    Lipids rich in polyunsaturated fatty acids are important nutrients. They are traditionally extracted from animals and plants but alternatively can be obtained from microbes through microbial lipid biotechnology. To make this process more economical, apart from strain engineering, the next frontier is through bioprocess and downstream innovation.

  • Designing Synthetic Microbial Consortia for Biofuel Production
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-03-02
    Yujia Jiang; Weiliang Dong; Fengxue Xin; Min Jiang

    Synthetic microbial consortia could efficiently produce biofuels from lignocellulose through labor division between different species. However, the incompatible growth conditions between lignocellulose degraders and biofuel producers might limit the overall conversion efficiency. We discuss recent examples of biofuel production using microbial consortia and suggest steps toward realistic implementation

  • Three-Dimensional Hierarchical Wrinkles on Polymer Films: From Chaotic to Ordered Antimicrobial Topographies
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-28
    Duy H.K. Nguyen; Olha Bazaka; Kateryna Bazaka; Russell J. Crawford; Elena P. Ivanova

    Microbial contamination of polymer surfaces has become a significant challenge in domestic, industrial, and biomedical applications. Recent progress in our understanding of how topographical features of different length scales can be used to effectively and selectively control the attachment and proliferation of different cell types has provided an alternative strategy for imparting antibacterial activity

  • Toward Sustainable Hydroxymethylfurfural Production Using Seaweeds
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-26
    Jae Bok Heo; Yong-Suk Lee; Chung-Han Chung

    Chemical manufacturing involves carbon sources releasing CO2 into the atmosphere. By contrast, seaweeds are carbon sinks that can absorb released CO2 and therefore have great potential for use as feedstocks in sustainable chemical manufacturing. In particular, seaweeds could contribute to mitigating vast amounts of global CO2 emissions. Accordingly, seaweeds could be an excellent candidate biomaterial

  • Developing a Computational Framework To Advance Bioprocess Scale-Up
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-25
    Guan Wang; Cees Haringa; Henk Noorman; Ju Chu; Yingping Zhuang

    Bioprocess scale-up is a critical step in process development. However, loss of production performance upon scaling-up, including reduced titer, yield, or productivity, has often been observed, hindering the commercialization of biotech innovations. Recent developments in scale-down studies assisted by computational fluid dynamics (CFD) and powerful stimulus–response metabolic models afford better

  • Intraoperative Bioprinting: Repairing Tissues and Organs in a Surgical Setting
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-24
    Yang Wu; Dino J. Ravnic; Ibrahim T. Ozbolat

    3D bioprinting directly into injured sites in a surgical setting, intraoperative bioprinting (IOB), is an effective process, in which the defect information can be rapidly acquired and then repaired via bioprinting on a live subject. In patients needing tissue resection, debridement, traumatic reconstruction, or fracture repair, the ability to scan and bioprint immediately following surgical preparation

  • Microbial Coculture for Flavonoid Synthesis
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-22
    Peng Xu; Monireh Marsafari; Jian Zha; Mattheos Koffas

    Flavonoids are plant-derived natural products with human health-promoting benefits. The modularity and complexity of the flavonoid biosynthetic pathway allow us to leverage the metabolic characteristics of distinct microbial hosts and install structural functionalities beyond what monocultures can achieve. We discuss the promising future of applying microbial cocultures to improve the cost-efficiency

  • Importance of Effective Regulation for the Chinese Genetic Testing Industry
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-22
    Xiaoqin Liu; Linzhe Jiang; Hongyan Wang; Chunyang Jiang

    The genetic testing industry is progressing rapidly. In particular, the demand of patients with tumors for diagnosis, treatment, and testing drives genetic testing to develop further. Simultaneously, the potential risks of genetic testing cause severe challenges to regulatory departments. In China, a targeted, clear, and unified management model remains absent.

  • Engineering Microphysiological Immune System Responses on Chips
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-18
    Chris P. Miller; Woojung Shin; Eun Hyun Ahn; Hyun Jung Kim; Deok-Ho Kim

    Tissues- and organs-on-chips are microphysiological systems (MPSs) that model the architectural and functional complexity of human tissues and organs that is lacking in conventional cell monolayer cultures. While substantial progress has been made in a variety of tissues and organs, chips recapitulating immune responses have not advanced as rapidly. This review discusses recent progress in MPSs for

  • Options to Reform the European Union Legislation on GMOs: Post-authorization and Beyond
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-12
    Dennis Eriksson; René Custers; Karin Edvardsson Björnberg; Sven Ove Hansson; Kai Purnhagen; Matin Qaim; Jörg Romeis; Joachim Schiemann; Stephan Schleissing; Jale Tosun; Richard G.F. Visser

    We discuss options to reform the EU genetically modified organism (GMO) regulatory framework, make risk assessment and decision-making more consistent with scientific principles, and lay the groundwork for international coherence. In this third of three articles, we focus on labeling and coexistence as well as discuss the political reality and potential ways forward.

  • Options to Reform the European Union Legislation on GMOs: Risk Governance
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-12
    Dennis Eriksson; René Custers; Karin Edvardsson Björnberg; Sven Ove Hansson; Kai Purnhagen; Matin Qaim; Jörg Romeis; Joachim Schiemann; Stephan Schleissing; Jale Tosun; Richard G.F. Visser

    Here, we discuss options to reform the EU genetically modified organism (GMO) regulatory framework, to make risk assessment and decision-making more consistent with scientific principles, and to lay the groundwork for international coherence. We discussed the scope and definitions in a previous article and, thus, here we focus on the procedures for risk assessment and risk management.

  • Options to Reform the European Union Legislation on GMOs: Scope and Definitions.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-12
    Dennis Eriksson,René Custers,Karin Edvardsson Björnberg,Sven Ove Hansson,Kai Purnhagen,Matin Qaim,Jörg Romeis,Joachim Schiemann,Stephan Schleissing,Jale Tosun,Richard G F Visser

    We discuss options to reform the EU genetically modified organisms (GMO) regulatory framework, make risk assessment and decision-making more consistent with scientific principles, and lay the groundwork for international coherence. The first in a three-part series, this article focuses on reform options related to the scope of the legislation and the GMO definition.

  • Microbial Chassis Development for Natural Product Biosynthesis.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-02-03
    Xianhao Xu,Yanfeng Liu,Guocheng Du,Rodrigo Ledesma-Amaro,Long Liu

    Engineering microbial cells to efficiently synthesize high-value-added natural products has received increasing attention in recent years. In this review, we describe the pipeline to build chassis cells for natural product production. First, we discuss recently developed genome mining strategies for identifying and designing biosynthetic modules and compare the characteristics of different host microbes

  • Geneva Statement on Heritable Human Genome Editing: The Need for Course Correction.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-27
    Roberto Andorno,Françoise Baylis,Marcy Darnovsky,Donna Dickenson,Hille Haker,Katie Hasson,Leah Lowthorp,George J Annas,Catherine Bourgain,Katherine Drabiak,Sigrid Graumann,Katrin Grüber,Matthias Kaiser,David King,Regine Kollek,Calum MacKellar,Jing-Bao Nie,Osagie K Obasogie,Mirriam Tyebally Fang,Gabriele Werner-Felmayer,Jana Zuscinova

    As public interest advocates, policy experts, bioethicists, and scientists, we call for a course correction in public discussions about heritable human genome editing. Clarifying misrepresentations, centering societal consequences and concerns, and fostering public empowerment will support robust, global public engagement and meaningful deliberation about altering the genes of future generations.

  • High-Throughput Screening Technology in Industrial Biotechnology.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-28
    Weizhu Zeng,Likun Guo,Sha Xu,Jian Chen,Jingwen Zhou

    Based on the development of automatic devices and rapid assay methods, various high-throughput screening (HTS) strategies have been established for improving the performance of industrial microorganisms. We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high diversity and the use of new detection methods to expand the search

  • Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-23
    James U Bowie,Saken Sherkhanov,Tyler P Korman,Meaghan A Valliere,Paul H Opgenorth,Hongjiang Liu

    Metabolic engineering efforts that harness living organisms to produce natural products and other useful chemicals face inherent difficulties because the maintenance of life processes often runs counter to our desire to maximize important production metrics. These challenges are particularly problematic for commodity chemical manufacturing where cost is critical. A cell-free approach, where biochemical

  • Toward an Optimized Process for Clinical Manufacturing of CAR-Treg Cell Therapy.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-22
    Enrico Fritsche,Hans-Dieter Volk,Petra Reinke,Mohamed Abou-El-Enein

    Chimeric antigen receptor (CAR) technology and its application to regulatory T cells (Tregs) has been hailed as the next scientific breakthrough in the field of cell and gene therapy. Merging the benefits of CAR technology with Tregs offers a novel and promising therapeutic option for durable reshaping of undesired immune responses following solid organ or hematopoietic stem cell transplantation, as

  • Enabling Technologies for Personalized and Precision Medicine.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-21
    Dean Ho,Stephen R Quake,Edward R B McCabe,Wee Joo Chng,Edward K Chow,Xianting Ding,Bruce D Gelb,Geoffrey S Ginsburg,Jason Hassenstab,Chih-Ming Ho,William C Mobley,Garry P Nolan,Steven T Rosen,Patrick Tan,Yun Yen,Ali Zarrinpar

    Individualizing patient treatment is a core objective of the medical field. Reaching this objective has been elusive owing to the complex set of factors contributing to both disease and health; many factors, from genes to proteins, remain unknown in their role in human physiology. Accurately diagnosing, monitoring, and treating disorders requires advances in biomarker discovery, the subsequent development

  • Phaeodactylum tricornutum: A Diatom Cell Factory.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-21
    Thomas Butler,Rahul Vijay Kapoore,Seetharaman Vaidyanathan

    A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing solutions. Diatoms display rich diversity and potential in this regard. We focus on Phaeodactylum

  • Controlling Voltage Reversal in Microbial Fuel Cells.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-21
    Bongkyu Kim,S Venkata Mohan,Deby Fapyane,In Seop Chang

    Microbial fuel cell (MFC) systems have been developed for potential use as power sources, along with several other applications, with bacteria as the prime factor enabling electrocatalytic activity. Limited voltage and current production from unit cells limit their practical applicability, so stacking multiple MFCs has been proposed as a way to increase power production. Special attention is paid to

  • Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-20
    Muhammad Nazeer Abbasi,Jun Fu,Xiaoying Bian,Hailong Wang,Youming Zhang,Aiying Li

    Microbial genomes encode many cryptic and uncharacterized biosynthetic gene clusters (BGCs). Exploiting this unexplored genetic wealth to discover microbial novel natural products (NPs) remains a challenging issue. We review homologous recombination (HR)-based recombineering, mediated by the recombinases RecE/RecT from Rac prophage and Redα/Redβ from lambda phage, which has developed into a highly

  • Synthetic Rewiring of Plant CO2 Sequestration Galvanizes Plant Biomass Production.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-17
    Muhammad Naseem,Özge Osmanoglu,Thomas Dandekar

    Synthetically designed alternative photorespiratory pathways in tobacco and rice plants have paved the way to enhanced plant biomass production. Likewise, some in vitro- and in vivo-tested carbon-concentrating cycles hold promise to increase plant biomass. We hypothesize a further increase in plant productivity if photorespiratory bypasses are integrated with carbon-concentrating cycles in plants.

  • 3D Printing in Suspension Baths: Keeping the Promises of Bioprinting Afloat.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-16
    Andrew McCormack,Christopher B Highley,Nicholas R Leslie,Ferry P W Melchels

    Extrusion-based 3D printers have been adopted in pursuit of engineering functional tissues through 3D bioprinting. However, we are still a long way from the promise of fabricating constructs approaching the complexity and function of native tissues. A major challenge is presented by the competing requirements of biomimicry and manufacturability. This opinion article discusses 3D printing in suspension

  • Gelatin Matrices for Growth Factor Sequestration.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-15
    Taneidra Buie,Joshua McCune,Elizabeth Cosgriff-Hernandez

    Gelatin is used in a broad range of tissue engineering applications because of its bioactivity, mild processing conditions, and ease of modification, which have increased interest in its use as a growth factor delivery vehicle. Traditional methods to control growth factor sequestration and delivery have relied on controlling hydrogel mesh size via chemical crosslinking with corollary changes to the

  • Protein Engineering for Improving and Diversifying Natural Product Biosynthesis.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-15
    Chenyi Li,Ruihua Zhang,Jian Wang,Lauren Marie Wilson,Yajun Yan

    Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosynthesis of natural products. Recently, protein engineering has become a favored method

  • Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-15
    Priyank Singhvi,Ankit Saneja,Sudeepa Srichandan,Amulya K Panda

    Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving tremendous attention for many diverse applications in the areas of industrial and medical biotechnology. Understanding the structure-function relationship of protein in IBs has recently created new possibilities in developing innovative isolation, solubilization, refolding, and purification processes for high-throughput

  • The Pathway Less Traveled: Engineering Biosynthesis of Nonstandard Functional Groups.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-15
    Morgan Sulzbach,Aditya M Kunjapur

    The field of metabolic engineering has achieved biochemical routes for conversion of renewable inputs to structurally diverse chemicals, but these products contain a limited number of chemical functional groups. In this review, we provide an overview of the progression of uncommon or 'nonstandard' functional groups from the elucidation of their biosynthetic machinery to the pathway optimization framework

  • Bioengineered 3D Models to Recapitulate Tissue Fibrosis.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-14
    Marta Sacchi,Ruchi Bansal,Jeroen Rouwkema

    Fibrosis, characterized by progressive tissue stiffening resulting in organ failure, is a growing health problem affecting millions of people worldwide. Currently, therapeutic options for tissue fibrosis are severely limited and organ transplantation is the only effective treatment for the end-stage fibrotic diseases with inherent limitations. Recent advancements in engineered 3D in vitro human disease

  • Stacked Bt Proteins Pose No New Risks to Nontarget Arthropods.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-30
    Jörg Romeis,Michael Meissle

    Concerns have been raised that multiple insecticidal proteins produced by genetically engineered (GE) crops may interact unexpectedly and pose new threats to biodiversity and nontarget organisms. We reviewed the literature to assess whether this concern is justified and whether the current regulatory framework needs to be adapted to address this concern.

  • Bioelectrofuel Synthesis by Nanoenzymes: Novel Alternatives to Conventional Enzymes.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-10
    Lakhveer Singh,Supriyanka Rana,Sveta Thakur,Deepak Pant

    Recent bioinspired efforts of designing novel nanoenzyme-based electrocatalysts are driven by the urgency of making bioelectrofuels more affordable and efficient. Unlike natural enzymes, nanoenzyme-modified electrodes with large surface areas enclose numerous biomimicking active sites to facilitate enhanced microbial growth followed by increased reactant-to-bioelectrofuel conversion.

  • Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-10
    Yu Wang,Liwen Fan,Philibert Tuyishime,Ping Zheng,Jibin Sun

    The increasing availability and affordability of natural gas has renewed interest in using methanol for bioproduction of useful chemicals. Engineering synthetic methylotrophy based on natural or artificial methanol assimilation pathways and genetically tractable platform microorganisms for methanol-based biomanufacturing is drawing particular attention. Recently, intensive efforts have been devoted

  • Intravesical Hydrogels as Drug Reservoirs.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-08
    Heping Qiu,Hui Guo,Di Li,Yuchuan Hou,Tairong Kuang,Jianxun Ding

    The complex environment in the bladder weakens the efficacy of intravesical therapy. Hydrogel-based drug delivery systems are poised to revolutionize the delivery of therapeutic agents to bladder lesion sites. This forum article highlights the prospective applications of hydrogels as drug reservoirs in treating chronic bladder diseases.

  • Metabolic Engineering of Escherichia coli for Natural Product Biosynthesis.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-07
    Dongsoo Yang,Seon Young Park,Yae Seul Park,Hyunmin Eun,Sang Yup Lee

    Natural products are widely employed in our daily lives as food additives, pharmaceuticals, nutraceuticals, and cosmetic ingredients, among others. However, their supply has often been limited because of low-yield extraction from natural resources such as plants. To overcome this problem, metabolically engineered Escherichia coli has emerged as a cell factory for natural product biosynthesis because

  • Ultrafast Photonic PCR Based on Photothermal Nanomaterials.
    Trends Biotechnol. (IF 13.747) Pub Date : 2020-01-06
    Minli You,Zedong Li,Shangsheng Feng,Bin Gao,Chunyan Yao,Jie Hu,Feng Xu

    Over the past few decades, PCR has been the gold standard for detecting nucleic acids (NAs) in various biomedical fields. However, there are several limitations associated with conventional PCR, such as complicated operation, need for bulky equipment, and, in particular, long thermocycling time. Emerging nanomaterials with photothermal effects have shown great potential for developing a new generation

  • Phosphoprotein Biosensors for Monitoring Pathological Protein Structural Changes.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-26
    Mostak Ahmed,Kevin M Koo,Paul N Mainwaring,Laura G Carrascosa,Matt Trau

    Current biotechnological developments are driving a significant shift towards integrating proteomic analysis with landmark genomic, methylomic, and transcriptomic data to elucidate functional effects. For the majority of proteins, structure and function are closely intertwined. Post-translational protein modifications (e.g., phosphorylation) leading to aberrantly active structures can originate a wide

  • Natural Medicinal Compounds in Bone Tissue Engineering.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-24
    Susmita Bose,Naboneeta Sarkar

    Recent advances in 3D printing have provided unprecedented opportunities in bone tissue engineering applications for producing a variety of complex patient-specific implants for the treatment of critical-sized bone defects. Natural medicinal compounds (NMCs) with osteogenic potential can be incorporated into these 3D-printed parts to improve bone formation and therefore enhance implant performance

  • Elastin Biomaterials in Dermal Repair.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-21
    Qingyun Wen,Suzanne M Mithieux,Anthony S Weiss

    Wound healing has historically relied on endogenous processes, but engineered materials are increasingly being used to assist tissue repair. Elastin is an essential functional component of the dermal extracellular matrix and is an important part of skin wound repair that encompasses an elastic dermis. Advances in modern technology have better elucidated the specific signaling factors and cells that

  • Microdroplet-Assisted Screening of Biomolecule Production for Metabolic Engineering Applications
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-10
    Emily K. Bowman, Hal S. Alper

    Success in synthetic biology and metabolic engineering is quickly becoming ‘test’ limited within the design–build–test cycle. Commonly used methods for high-throughput screening, including fluorescence-activated cell sorting (FACS) and microtiter plates, have intracellular product and throughput limitations. A growing alternative to these challenges is the use of microfluidic microdroplet-based methods

  • Cancer Immunoimaging with Smart Nanoparticles
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-05
    Yu-Chuan Ou, Xiaona Wen, Rizia Bardhan

    Dynamic immunoimaging in vivo is crucial in patient-tailored immunotherapies to identify patients who will benefit from immunotherapies, monitor therapeutic efficacy post treatment, and determine alternative strategies for nonresponders. Nanoparticles have played a major role in the immunotherapy landscape. In this review, we summarize recent findings in immunoimaging where smart nanoparticles target

  • Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-12-04
    Laura A.E. Van Poelvoorde, Xavier Saelens, Isabelle Thomas, Nancy H. Roosens

    Next-generation sequencing (NGS) can enable a more effective response to a wide range of communicable disease threats, such as influenza, which is one of the leading causes of human morbidity and mortality worldwide. After vaccination, antivirals are the second line of defense against influenza. The use of currently available antivirals can lead to antiviral resistance mutations in the entire influenza

  • Leveling Up Hydrogels: Hybrid Systems in Tissue Engineering.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-11-29
    Sara C Neves,Lorenzo Moroni,Cristina C Barrias,Pedro L Granja

    Hydrogels can mimic several features of the cell native microenvironment and have been widely used as synthetic extracellular matrices (ECMs) in tissue engineering and regenerative medicine (TERM). However, some applications have specifications that hydrogels cannot efficiently fulfill on their own. Incorporating reinforcing structures like fibrous scaffolds or particles into hydrogels, as hybrid systems

  • Unearthing Hidden Chemical Potential from Discarded Actinobacterial Libraries.
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-11-27
    Marshall L Timmermans,Avena C Ross

    The redundancy of natural product biosynthesis in microbes poses a practical challenge for discovering new antimicrobial compounds from bacteria. The recent application of clustered regularly interspaced short palindromic repeats (CRISPR) technology by Culp et al. to inactivate the production of abundant antibiotics generates a metabolic clean slate for the detection and/or isolation of new and less

  • Gut Check Time: Antibiotic Delivery Strategies to Reduce Antimicrobial Resistance
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-11-19
    Stephen A. Kelly, Aoife M. Rodgers, Séamus C. O’Brien, Ryan F. Donnelly, Brendan F. Gilmore

    Antimicrobial resistance (AMR) has developed into a huge threat to global health, and reducing it is an urgent priority for public health authorities. The importance of a healthy and balanced gut microbiome has been identified as a key protective factor against AMR development, but this can be significantly affected by antibiotic therapy, resulting in dysbiosis and reduction of taxonomic richness.

  • Hands-On Introduction to Synthetic Biology for Security Professionals
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-07-15
    Neil R. Adames, Jenna E. Gallegos, Sonia Y. Hunt, William K. So, Jean Peccoud

    The rapid pace of life sciences innovations and a growing list of nontraditional actors engaging in biological research make it challenging to develop appropriate policies to protect sensitive infrastructures. To address this challenge, we developed a five-day awareness program for security professionals, including laboratory work, site visits, and lectures.

  • Synthetic Biology and the United Nations
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-06-27
    Hung-En Lai, Caoimhe Canavan, Loren Cameron, Simon Moore, Monika Danchenko, Todd Kuiken, Zuzana Sekeyová, Paul S. Freemont

    Synthetic biology is a rapidly emerging interdisciplinary field of science and engineering that aims to redesign living systems through reprogramming genetic information. The field has catalysed global debate among policymakers and publics. Here we describe how synthetic biology relates to these international deliberations, particularly the Convention on Biological Diversity (CBD).

  • Paradigm Shift in Algal H2 Production: Bypassing Competitive Processes
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-06-04
    Szilvia Z. Tóth, Iftach Yacoby

    Hydrogen is a promising energy carrier, but producing it sustainably remains a challenge. Green algae can produce hydrogen photosynthetically using their efficient but oxygen-sensitive hydrogenases. Recent strategies aiming to bypass competing processes provide a promising route for scaling up algal hydrogen production.

  • Antimicrobial Inks: The Anti-Infective Applications of Bioprinted Bacterial Polysaccharides
    Trends Biotechnol. (IF 13.747) Pub Date : 2019-06-03
    Ronan R. McCarthy, Muhammad Wajid Ullah, Eujin Pei, Guang Yang

    Bioprinting is a rapidly emerging technology with the potential to transform the biomedical sector. Here, we discuss how a range of bacterial polysaccharides with antibiofilm and antibacterial activity could be used to augment current bioink formulations to improve their biocompatibility and tackle the spread of antibiotic-resistant infections.

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全球疫情及响应:BMC Medicine专题征稿