-
Letter to the Editor Biotech. Histochem. (IF 1.203) Pub Date : 2020-11-06 Armen Nersesyan
(2021). Letter to the Editor. Biotechnic & Histochemistry: Vol. 96, No. 2, pp. 83-84.
-
Quirks of dye nomenclature. 16. Dyes, and a pigment, named after places Biotech. Histochem. (IF 1.203) Pub Date : 2021-01-12 C. J. Cooksey
ABSTRACT Many dyes produced during the 19th century were named after locations. Manufacturers proliferated the number of synonyms used and in time, the original names were forgotten. Therefore, in the headings below, the original names are followed by the current name(s) in parentheses. The stories of some of these dyes that survived into the 21st century are recounted here. Numerical identity data
-
Protective effect of benfotiamine on methotrexate induced gastric damage in rats Biotech. Histochem. (IF 1.203) Pub Date : 2020-12-16 S. Koc; M. A. Erdogan; E. Erdogan; A. Yalcin; A. Turk; M. M. Erdogan
ABSTRACT Methotrexate (MTX) is widely used for treating cancers and inflammatory diseases; it is a potential anti-metabolite and folate antagonist. We investigated potential protective effects of benfotiamine on MTX damage. We used a rat model of MTX induced gastric injury to assess changes in gastric histopathology, oxidative stress and visfatin levels due to MTX treatment. Rats were divided into
-
Photodynamic inactivation of Staphylococcus aureus using tetraethylene glycol-substituted Zn(II) phthalocyanine Biotech. Histochem. (IF 1.203) Pub Date : 2020-12-16 Ergun Mete; Nilgün Kabay; Fabienne Dumoulin; Vefa Ahsen; Sinem Tuncel Kostakoğlu; Çağrı Ergin
ABSTRACT Methicillin resistant Staphylococcus aureus infections are increasing, especially in intensive care units. A new method for photodynamic inactivation (PDI) generates reactive oxygen species by photosensitization to kill bacteria. We investigated the PDI effect of tetraethylene glycol-substituted Zn(II) phthalocyanine (TEG-P) on S. aureus strains including two standards (ATCC 25923 and ATCC
-
Protective effect of luteolin on acute lung injury in a rat model of sepsis Biotech. Histochem. (IF 1.203) Pub Date : 2020-11-12 Demet Celebi; Pelin Aydin; Irfan Cinar; Zerrin Kutlu; Ilknur Calik; Zekai Halici; Dilek Bilici; Zafer Bayraktutan
ABSTRACT We investigated the effects of luteolin (LUT) treatment on acute lung injury caused by cecal ligation and puncture (CLP) induced septic rats. We also investigated the relation between LUT and the cytokines, interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α). LUT was administered 1 h after CLP surgery. Administration of LUT reduced the glutathione level and superoxide dismutase activity
-
Correction Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-30
(2020). Correction. Biotechnic & Histochemistry. Ahead of Print.
-
Effects of oxidative stress and apoptosis on vascularity and viability of perforator flaps Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-27 Ulaş Bali; Işıl Aydemir; Yavuz Keçeci; Levent Yoleri; Mehmet İbrahim Tuğlu
ABSTRACT We investigated lateral thoracic and posterior thigh perforator flaps for viability, vascularization, perfusion and apoptosis in a rat model. Wistar albino rats were divided into six groups: lateral thoracic artery perforator flap (LTPF) sham, 3 × 2 cm2 LTPF, 3 × 6 cm2 LTPF, posterior thigh perforator flap (PTPF) sham, 3 × 2 cm2 PTPF, and 3 × 6 cm2 PTPF. Flap viability was determined on postoperative
-
Nesfatin-1 and neuronostatin neurons are co-expressed with glucocorticoid receptors in the hypothalamus Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-15 G Ekizceli; KZ Halk; Z Minbay; O Eyigor
ABSTRACT Nesfatin-1 and neuronostatin in the central nervous system participate in regulating stress responses. Glucocorticoid hormones affect the brain through glucocorticoid receptors (GR). We investigated in the rat the possibility of co-localizing nesfatin-1 and neuronostatin neurons in hypothalamic areas with GR. using immunohistochemistry. We counted nesfatin-1 and neuronostatin stained neurons
-
Can renalase be a novel candidate biomarker for distinguishing renal tumors? Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-14 RF Akkoc; S. Aydin; M. Goksu; S. Ozcan Yildirim; Y. Eroksuz; M. Ogeturk; K. Ugur; A. F. Dagli; B. Yakar; İ. Sahin; S. Aydin
ABSTRACT Renalase (RNLS) is synthesized mainly in renal tissues. The function of RNLS in cancerous renal tissues has not been investigated. We investigated the synthesis of RNLS in chromophobe renal cell carcinoma, papillary renal cell carcinoma and clear cell renal cell carcinoma with Fuhrman grades (FG): FG1, nucleoli are absent or inconspicuous and basophilic; FG2, nucleoli are conspicuous and eosinophilic
-
Hyaluronic acid with antioxidants improve wound healing in rats Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-13 Mehmet Murat Taskan; Hatice Balci Yuce; Ozkan Karatas; Fikret Gevrek; Gozde Isiker Kara; Melike Celt; Elif Sirma Taskan
ABSTRACT Hyaluronic acid (HA) is found in connective tissue and participates in wound healing. We investigated the efficacy of a HA gel (2% hyaluronic acid; 1% antioxidants, coenzyme Q10 and vitamin E; and 5% benzocaine) on healing of palatal wounds in rats. We established two groups of rats: a control group treated with vehicle and an HA group treated with HA gel. The control group was divided into
-
Expression of PDGF-A, PDGFRA, integrin subunit alpha V and selectin E is increased in canine cutaneous fibrosarcomas Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-09 Ishtiaq Ahmed; Mahmut Sozmen
ABSTRACT We investigated the expression of platelet derived growth factor alpha (PDGFA); its receptor, PDGFRA; integrin subunit alpha V; and selectin E in cutaneous fibrosarcomas in dogs. Ten cases of canine fibrosarcomas were obtained from the archive of the Department of Pathology, Ondokuz Mayis University, Samsun. Tissue sections were cut and stained with hematoxylin and eosin, Alcian blue-periodic
-
Expression of angiogenic growth factors in canine squamous cell cancers Biotech. Histochem. (IF 1.203) Pub Date : 2020-10-02 M. Sözmen; A. K. Devrim; M. Sudağıdan; Y. B. Kabak; F. Yıldırım
ABSTRACT Skin and subcutaneous tissue tumors are the most common neoplasms in dogs. The most common sites of origin in dogs include digits, skin and the oral cavity including cheek and retromandibular area. We investigated canine squamous cell carcinoma (SCC) samples from 15 dogs and classified them histopathologically according to the degree of differentiation. bFGF, VEGF-C, TGF-β, PDGF-A, PDGF-C
-
Letter to the Editor Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-28 Katyane Negreiros Campos; Maria Esther Suarez Alpire; Marcelo Jose Dias Silva; Luciana Pellegrini Pisani; Daniel Araki Ribeiro
(2020). Letter to the Editor. Biotechnic & Histochemistry: Vol. 95, No. 8, pp. 563-564.
-
Reply to Letter to the Editor by Dr. Ribeiro Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-28 Okan Sancer; Muhammet Yusuf Tepebaşı
(2020). Reply to Letter to the Editor by Dr. Ribeiro. Biotechnic & Histochemistry: Vol. 95, No. 8, pp. 565-566.
-
Immunohistochemical and histophysiological study of prolonged use of nandrolone on reproductive organs and fertility. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-28 Laís Caroline da Silva Santos,Clovis José Cavalcanti Lapa Neto,Anthony Marcos Gomes Dos Santos,Ketsia Sabrina do Nascimento Marinho,Bruno José do Nascimento,Erique Ricardo Alves,Álvaro Aguiar Coelho Teixeir,Valéria Wanderley-Teixeira
ABSTRACT We investigated possible changes in morphology and immunohistochemistry of the uterus and ovaries of rats caused by nandrolone (ND); we also investigated effects on fertility. We used 30 rats divided into three experimental groups: control (C), control vehicle (CV) and 5 mg/kg ND. Rats treated with ND exhibited loss of estrous cyclicity with predominance of the estrus phase, increased body
-
Expression of the insulin-like growth factor (IGF) gene family in feline uterus during pregnancy. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-24 Özgecan Korkmaz Ağaoğlu,Ali Reha Ağaoğlu,Özlem Özmen,Mustafa Saatci,Sabine Schäfer-Somi,Selim Aslan
Members of the IGF gene family participate in cell differentiation and proliferation during pregnancy. We used 35 cats assigned to experimental groups (G) based on pregnancy stages: G1, pre-implantation; G2, implantation; G3, early pregnancy; G4, mid-pregnancy; G5, nonpregnant. Quantitative polymerase chain reaction (qPCR) and immunohistochemistry were used to analyze the expression of the IGF gene
-
Tunicamycin induced endoplasmic reticulum stress in the small intestine. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-23 Zübeyde Öztel,Sibel Gazan,Erdal Balcan
Because the small intestine is exposed to variety of foreign substances, it participates in host immune response. We investigated whether the expression levels of intestinal MAdCAM-1, PECAM-1 (CD31) and CAV-1 are affected by endoplasmic reticulum (ER) stress following brief treatment with tunicamycin (TN). We administered a single dose of TN intraperitoneally. Twenty-four hours later, MAdCAM-1, PECAM-1
-
Effects of vitamin E in a glucocorticoid induced cataract model in chicken embryos. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-22 A Vurmaz,A Ertekin,M Cem Sabaner,E Atay,E Bozkurt,A Bilir
We investigated the antioxidant effects of vitamin E on a glucocorticoid (GC) induced model of cataracts in chick embryos. We used 70 fertilized eggs divided into seven groups as follows: phosphate-buffered saline (PBS) group, olive oil treatment (OO) group, hydrocortisone treatment (HC) group, olive oil and hydrocortisone treatment (OO + HC) group, 50 mg/kg vitamin E and hydrocortisone treatment (HC
-
Model for type 2 diabetes exhibits changes in vascular function and structure due to vascular oxidative stress and inflammation. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-22 Ahmad Khusairi Azemi,Siti Safiah Mokhtar,Low Jen Hou,Sharifah Emilia Tuan Sharif,Aida Hanum Ghulam Rasool
We used a type 2 diabetes rat model produced by a high fat diet (HFD) followed by low dose streptozotocin (STZ) to study diabetic vasculopathy. Animals were evaluated for early vascular structural changes, endothelial function, inflammation, lipid profile and oxidative stress. We used 20 male Sprague-Dawley rats divided equally into control and diabetic groups. Diabetic rats were fed an HFD for 4 weeks
-
Identification of different cell clusters in the endothelium of atherosclerotic vessels and determination of inter-cluster gradient of proliferative and inflammatory activity as new diagnostic markers. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-17 P V Nikitin,M V Ryzhova,S A Galstyan,D S Kim,I V Zubova,E A Khokhlova,S V Shugay
To characterize atherogenesis functionally, we studied the functional heterogeneity of endotheliocytes in carotid vessels with atherosclerotic plaques and identified several distinct cell clusters. We measured the Ki-67 labeling index (Ki-67 LI), percentage of Bcl-2 cells (CP) and expression of CCL5, IL 6 and VCAM1 in each cell cluster. We also investigated how these indicators change when the plaque
-
Effects of diabetes on apoptosis and mitosis in rat hippocampus. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-17 Serpil Ünver Saraydin,Berna Özdenoglu Kutlu,Dursun Saraydın
Diabetes contributes to neurological dysfunction including peripheral nerve diseases, stroke and dementia. We investigated the effects of diabetes on apoptosis and mitosis in the hippocampal CA1 region. Rats were given diabetes by injection of streptozotocin (STZ). The mass and blood glucose levels of the rats were measured until day 7 of the experiment. The loss of mass index was approximately 10%
-
Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-14 H Asci,O Ozmen,Y Erzurumlu,H B Savas,Esra Nurlu Temel,P Icten,N Hasseyid
We investigated the antioxidant, anti-inflammatory and anti-apoptotic effects of pregabalin (PREG) on lipopolysaccharide (LPS) induced sepsis related cardiotoxicity via NF-kβ pathways. We used 24 female Wistar albino rats divided into three groups: control, LPS treated and LPS + PREG treated. Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), tumor necrosis factor
-
Alpha lipoic acid attenuates iron induced oxidative acute kidney injury in rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-14 Zahide Cavdar,Mehmet Asi Oktan,Cemre Ural,Ayse Kocak,Meryem Calisir,Cihan Heybeli,Serkan Yildiz,Seda Ozbal,Sevki Arslan,Bekir Ugur Ergur,Osman Yilmaz,Caner Cavdar
Iron has been implicated in oxidative tissue injury owing to its ability to generate reactive oxygen species (ROS). We investigated the reno-protective effects of alpha lipoic acid (ALA) by investigating its effects on the kidney isoform of NADPH oxidase (Nox4) and the specific signaling pathways, p38 MAPK and PI3K/Akt, which participate in apoptosis and survival, respectively. We established four
-
Role of bone marrow-derived mesenchymal stem cells in alleviating pulmonary epithelium damage and extracellular matrix remodeling in a rat model of lung fibrosis induced by amiodarone. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-10 Alyaa S Abdel Halim,Hanaa H Ahmed,Hadeer A Aglan,Fatma F Abdel Hamid,Mohamed R Mohamed
The therapeutic role of mesenchymal stem cells (MSCs) in cases of amiodarone (AD) induced pulmonary fibrosis (PF) has not been well studied. Also, the period required by MSCs to attain full therapeutic effectiveness has not yet been assessed. We investigated the potential curative effect of bone marrow-derived MSCs (BM-MSCs) and conditioned media (CM) from BM-MSCs on AD induced PF by focusing on pulmonary
-
Composition of abomasal mucins in hair goats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-03 Zelal Karakoç,Uğur Topaloğlu,Muzaffer Aydın Ketani
The mucosal epithelium of the digestive tract is covered by a layer of mucusthat protects against chemical, enzymatic and mechanical damage, and pathogenic microorganisms. We investigated the composition of the mucin in the abomasum of goat kids using histochemistry and immunohistochemistry. We used 6 − 8-month-old goat kids; samples were collected from the cardia, fundus and pylorus regions of the
-
Analysis of KRAS mutations in circulating tumor DNA and colorectal cancer tissue. Biotech. Histochem. (IF 1.203) Pub Date : 2020-09-02 Yankui Liu,Longhai Li,Yu Tian,Xiao Zhu,Aijuan Sun,Yulong Sun,Yan Qiao,Xiaowei Qi,Tao Wang
The mutation status of KRAS is important for anti-EGFR therapy in colorectal cancer (CRC) patients; however, detection of KRAS mutations in circulating tumor DNA (ctDNA) is problematic. We investigated tissue and plasma assays for KRAS mutations in CRC patients. The KRAS status of 407 CRC patients was evaluated using integration of amplification refractory mutation system polymerase chain reaction
-
Correction. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-21
(2020). Correction. Biotechnic & Histochemistry: Vol. 95, No. 7, pp. 561-561.
-
Crocin ameliorates oxidative stress and suppresses renal damage in streptozotocin induced diabetic male rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-24 Ayşegül Kapucu
ABSTRACT Diabetes mellitus (DM) is a prevalent metabolic disorder with complications including nephropathy, neuropathy, retinopathy and peripheral vascular disease. Crocin is a water soluble carotenoid that exhibits strong antioxidant activity. I investigated the potential hypoglycemic and reno-protective effects of crocin for type 1 diabetic male rats using periodic acid-Schiff and hematoxylin staining;
-
Modified Papanicolaou staining for oral swab samples stored long term. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-21 Srijayaprakash B Uppada,Lepakshi S V Madduri,Sravani Singu,Brooke Lawson,Linda Bauer,Alison Freifeld,Vijaya R Bhatt,Siddappa N Byrareddy
Pathologists collect swab samples for Papanicolaou (Pap) staining to diagnose various diseases including cancer and HIV. Time constraints and limited resources, may preclude staining a sample immediately. To re-confirm results, samples must be frozen for later analysis. We present a method for Pap staining cells that have been stored long term. An effective method for Pap staining of frozen cells should
-
The neutral red assay can be used to evaluate cell viability during autophagy or in an acidic microenvironment in vitro. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-03 Jorge G Gomez-Gutierrez,Neal Bhutiani,Molly W McNally,Phillip Chuong,Wenyuan Yin,Meredith A Jones,Matthew R Zeiderman,William E Grizzle,Lacey R McNally
Harsh conditions within the tumor microenvironment, such as hypoxia and extracellular acidic pH (pHe), inactivate some chemotherapies, which results in limited or no cytotoxicity. Standard MTT, ATPlite and protease assays that are used to determine the potency of newly developed drugs often give erroneous results when applied under hypoxic or acidic conditions. Therefore, development of a cytotoxicity
-
Immunoexpression of galectin-3 and its potential relation to hypoxia-inducible factor-1α in ameloblastomas. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-03 V Pereira-Prado,G Vigil-Bastitta,C Sánchez-Romero,M Arocena,N Molina-Frechero,R González-González,M Meleti,R Bologna-Molina
In tumor biology, hypoxia triggers signaling pathways that induce transcription of genes related to angiogenesis, metastasis, glucose metabolism and apoptosis. We investigated the expression of hypoxia related proteins, galectin-3 (Gal-3) and hypoxia-inducible factor-1α (HIF-1α), in conventional (CA) and unicystic ameloblastomas (UA). We applied immunohistochemistry for Gal-3 and HIF-1α to 72 cases
-
Comparison of DNA damage and proliferative capacities in smear samples of HPV positive and negative patients by micronucleus counting and AgNOR staining. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-03 Okan Sancer,Mümtaz Cem Şirin,Buket Arıdoğan,Emel Sesli Çetin,Ahmet Yiğit,Muhammet Yusuf Tepebaşı,Pınar Aslan Koşar
Human papillomavirus (HPV) is believed to cause cervical cancer. Thousands of women develop cancer and other diseases caused by HPV each year. HPV 16 and 18 types are found in approximately 70% of cervical cancers. Micronuclei are small chromosomal fragments that are considered indicators of DNA damage. AgNOR positive dots are useful for assessing proliferation. We investigated the relation between
-
The relation between the ghrelin receptor and FOXP3 in bladder cancer. Biotech. Histochem. (IF 1.203) Pub Date : 2020-08-03 Monireh Karimi,Masoud Heshmati,Soheila Fattahi,Nader Bagheri,Faramarz Mohammad Alibeigi,Fatemeh Taheri,Maryam Anjomshoa,Mohammad-Saeid Jami,Mahdi Ghatreh Samani
Immune responses play an important role in the fate of bladder cancer tumors. Treg cells are immunosuppressive and down-regulate the proliferation of effector T cells, which favor tumor survival. Ghrelin is a hormone that stimulates release of growth hormone and anti-inflammatory response to cancer cells. Ghrelin also is a gastrointestinal hormone that regulates immune responses via the growth hormone
-
Topical administration of hydroethanolic extract of Trifolium pratense (red clover) accelerates wound healing by apoptosis and re-epithelialization. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-27 R Manzoureh,M R Farahpour
We investigated the wound healing effects of an ointment prepared from a hydroethanolic extract of Trifolium pratense (red clover) in Wistar rats. An animal model was prepared by making 314 mm2 full thickness skin excisions in all animals. Rats were divided into four experimental groups. Group 1 was a non-intervention control. Groups 2, 3 and 4 were treated topically with ointments containing 1.5,
-
Distribution of spleen connective tissue fibers in diabetic and vitamin C treated diabetic rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-22 Dilşad Özerkan,Nesrin Özsoy,Suna Cebesoy,Çiğdem Özer
We investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson’s
-
The hydroalcoholic extract of Zingiber officinale diminishes diazinon-induced hepatotoxicity by suppressing oxidative stress and apoptosis in rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-16 Saeed Yaghubi Beklar,Maedeh Hamzeh,Abbasali Karimpour Malekshah,Fereshteh Talebpour Amiri
Diazinon (DZN) is an organophosphate insecticide that affects the liver adversely. Ginger exhibits antioxidant properties. We investigated the hepatoprotective effects of an ethanolic extract of ginger root on DZN induced hepatotoxicity. We measured total phenolics and flavonoids in the hydroalcoholic extract. We used Wistar rats divided into four groups: control, 100 mg/kg/day ginger by gavage, 10
-
Dynamics of breast tumor incidence, tumor volume and serum metabolic hormones in calorie restricted rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-16 Ece Koc Yildirim,Muharrem Balkaya
Reports of the effects of calorie restriction on tumors after the tumor has developed are uncommon and limited to a few tumor types. We investigated the effects of calorie restriction on tumor growth in breast cancer after tumor growth had progressed. We used the N-nitroso-N-methylurea (NMU) induced breast cancer model in rats. In addition to a healthy control group (C), rats with 10 − 12 mm tumors
-
Bavachinin mitigates DMH induced colon cancer in rats by altering p53/Bcl2/BAX signaling associated with apoptosis. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-15 Chun Zhao,Balaram Ghosh,Tania Chakraborty,Souvik Roy
Bavachinin is a flavanone obtained from the Chinese herb, Fructus Psoraleae. Flavonoids and flavanones are recognized as cancer preventive agents. We investigated the anticancer properties of bavachinin using a model of dimethylhydrazine (DMH and dextran sodium sulfate (DSS) induced rat colon cancer. We investigated aberrant crypt foci (ACF), hyperplastic lesions, catalase (CAT), superoxide dismutase
-
Effects of diabetes on nitric oxide synthase in rat uterus. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-13 Derya Karabulut,Mehmet Fatih Sonmez
Diabetes mellitus (DM) is a common metabolic disorder with complications including nephropathy, cardiomyopathy, neuropathy and infertility. We investigated nitric oxide synthase (NOS) expression in uterine tissue of diabetic rats. The rats were assigned randomly to four groups of ten: group C, control; group 1, diabetic for 1 month; group 2, diabetic for 2 months; group 3, diabetic for 3 months. DM
-
Metformin alleviates experimental colitis in mice by up-regulating TGF-β signaling. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-13 Xuewei Liu,Zheng Sun,Haifeng Wang
ABSTRACT Ulcerative colitis is an inflammatory condition of the colon. Metformin, an antidiabetic drug, can suppress the inflammatory cascade. We investigated the effects of metformin on experimental colitis. Experimental colitis was induced in mice using dextran sulfate sodium. We used three groups of mice: untreated control, saline treated and metformin treated. We measured weight, disease activity
-
Hematoxylin in the 21st century. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-10 C J Cooksey
Hematoxylin continues to be a popular substance in the 21st century. I present here some recent developments including the use and misuse of the terms, hematoxylin and hemalum, in biological staining. The medical use of hematoxylin and its application as a dye for hair or textiles continues to be of interest. Unusual applications including its use as a biosensor and for study of vibrational properties
-
A histopathological and stereological study of the effects of acetylsalicylic acid on doxorubicin-induced hepatotoxicity in mice. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-09 Ayşe Başardı Gökçe,Banu Eren,Dilek Sağır,Burcu Demirel Yılmaz
Doxorubicin (Dox) is an anthracycline antibiotic with antineoplastic activity. Acetylsalicylic acid (Asa) is recommended for use as a prophylactic for thromboembolism during treatment of cancers. We investigated liver toxicity due to combined use of Dox and Asa in chemotherapy regimens. We used 140 Swiss albino mice divided into four main groups: control, Dox, Asa, and Dox + Asa. Each group was subdivided
-
Immunohistochemical profile of the anti-apoptosis, apoptosis and proliferation markers Bcl-2, caspase-3, p53, and Ki-67 in botryoid odontogenic cysts compared to lateral periodontal cysts and gingival cysts of the adult. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-09 John R Basile,James T Castle,Robert S Redman
We investigated the differences in growth and rates of recurrence of the botryoid odontogenic cyst (BOC) and the less aggressive lateral periodontal cyst (LPC) and gingival cyst of the adult (GCA). We compared the immunohistochemical expression of selected biomarkers of apoptosis and proliferation and of regulators of their activity. Sections from archival paraffin blocks of 15 BOCs, six GCAs, six
-
Effects of carbon dioxide pneumoperitoneum on exocrine and endocrine functions, and oxidative state of rat pancreas. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-09 Sefa Sag,Mustafa Imamoglu,Haluk Sarihan,Esin Yulug,Ahmet Alver,Sukran Geze Saatci,Ali Cay
We investigated the effects of increased intra-abdominal pressure during laparoscopy on the endocrine and exocrine functions, oxidative stress and histopathology of the pancreas in rats. We established three experimental groups of eight animals. Group 1 was the untreated control. Forth other two groups, pneumoperitoneum with CO2 was established for 60 min at 6 mm Hg for group 2 and 12 mm Hg for group
-
Silencing Stat3 inhibits viability and induces apoptosis in BGC-823 human gastric cancer cell line. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-03 Yaming Xu,Shini Feng,Bing Niu
ABSTRACT Gastric cancer (GC) is characterized by infrequent early diagnosis, poor prognosis and high mortality. Signal transducer and activator of transcription 3 (Stat3) and signal transducer and activator of transcription 5b (Stat5b) play multiple roles in the development and progression of many human cancers. We investigated the effects of silencing Stat3 and Stat5b on the viability and apoptosis
-
Physiochemical properties of copper doped calcium sulfate in vitro and angiogenesis in vivo. Biotech. Histochem. (IF 1.203) Pub Date : 2020-07-02 Lei Huang,Yong Heng Xie,Hai Bo Xiang,Yi Long Hou,Bin Yu
ABSTRACT Bone cements were prepared by mixing calcium sulfate and copper sulfate in various proportions. We examined physical and physicochemical properties of the copper doped calcium sulfates and the effects of the cements on angiogenesis in vivo. Rod shaped calcium sulfate crystals were visible by scanning electron microscopy in the cement that contained no copper sulfate, whereas plate-like crystals
-
Micro- and macroanatomical features of the uropygial gland of duck (Anas platyrhynchos) and pigeon (Columba livia) Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-30 Amin Hassanin; Mahmoud Shoeib; Diaa Massoud
We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was
-
Morin treatment for acute ethanol exposure in rats. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-29 Anbu Singaravelu,Karthikkumar Venkatachalam,Richard L Jayaraj,Padma Jayabalan,Saravanan Nadanam
Ethanol intoxication increases oxidative stress and pro-inflammatory proteins, which cause neurodegeneration. Morin is a natural flavonoid obtained from plants of the Moraceae family that exhibits antioxidant, anti-inflammatory, hepatoprotective, anticancer and cardioprotective properties. We investigated the neuroprotective effect of morin on ethanol intoxicated rats. Rats exposed to ethanol exhibit
-
Early morphological and apoptotic responses of bird erythrocytes to thermal stress. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-29 Aleksandra Szabelak,Adam Bownik,Sebastian Knaga,Kornel Kasperek
Little is known about early indicators of heat stress in bird erythrocytes. We investigated the effects of elevated temperatures on the morphology and cellular responses of avian erythrocytes. Hen red blood cells were subjected to 22–45 °C temperatures for 1 h and 4 h, then stained and examined by light microscopy to assess morphological alterations. Cell viability, cytotoxicity and caspases 3 and
-
Investigation of EGFR/pi3k/Akt signaling pathway in seminomas. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-29 F Guerra,S Quintana,S Giustina,G Mendeluk,L Jufe,M A Avagnina,L B Díaz,L A Palaoro
ABSTRACT Activation of the receptor for epidermal growth factor (EGFR) in some testicular tumors activates several signaling pathways. Some components of these pathways are phosphorylated or mutated in testicular germ tumors (TCGT), including EGFR, Kirstein ras oncogen (KRAS) and cell surface protein of the germ cell (KIT). The latter two activate RAF ⁄MEK⁄ERK and PI3 K⁄AKT, and interconnect with the
-
Effects of karanjin on dimethylhydrazine induced colon carcinoma and aberrant crypt foci are facilitated by alteration of the p53/Bcl2/BAX pathway for apoptosis. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-25 Jingyu Zhang,Yandong Xie,Qingling Fan,Cheng Wang
We investigated the effects of karanjin on dimethylhydrazine (DMH) induced colon cancer in rats. Male Wistar rats were injected with DMH followed by dextran sodium sulfate in drinking water for 7 days. Karanjin at doses of 50,100 and 200 mg/kg was administered orally for 18 weeks. Colon tissues were investigated using TUNEL analysis of apoptosis; histopathological assessment including number of aberrant
-
An immunohistochemical study of the effects of orexin receptor blockade on phospholipase C-β3 level in rat hippocampal dentate gyrus neurons. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-25 Zeinab Mousavi,Masoumeh Kourosh-Arami,Monireh Mohsenzadegan,Alireza Komaki
Orexin-A (hypocretin-1) is a neuropeptide that is produced in the lateral hypothalamic area (LHA) and promotes widespread cortical activation. We investigated the effect of SB-334867, a selective orexin receptor 1 (OXR1) antagonist, on phospholipase C-β3 (PLCβ3) level using slices of rat hippocampus preparations and immunohistochemistry. We used three Wistar rats in each of three groups. The control
-
Effects of carnosine on apoptosis, transient receptor potential melastatin 2, and betatrophin in rats exposed to formaldehyde. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-25 R F Akkoc,M Ogeturk,S Aydin,T Kuloglu,S Aydin
We investigated the effects of exposure to formaldehyde on transient receptor potential melastatin 2, betatrophin, total oxidant status and total antioxidant status in rat liver and kidney tissues. We also investigated the effects of carnosine on formaldehyde treated animals. We used 28 male rats divided ramdomly into four groups of seven: untreated control group, carnosine treated group, formaldehyde
-
Rapid, practical and safe isolation of adipose derived stem cells. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-23 Ece Çerçi,Hatice Erdost
ABSTRACT We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group
-
Effect of geldanamycin on the expression of the matrix molecules and angiogenetic factors in a gastric cancer cell line. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-23 T Gürpınar,F Kosova,F O Kurt,S U Cambaz,A T Yücel,N Umur,M I Tuğlu
ABSTRACT Angiogenesis is the formation of new blood vessels. Angiogenesis affects cancer growth and is a useful target for cancer therapeutics. The effects of geldanamycin on angiogenesis in cases of gastric cancer are poorly understood. We investigated the effects of different doses of 17-allylamino-17-demethoxygeldanamycin (17-AGG), a semi-synthetic derivative of geldanamycin, on the interactions
-
Efficacy of modified cell block cytology compared to fine needle aspiration cytology for diagnostic oral cytopathology. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-18 M S Sale,V V Kulkarni,P V Kulkarni,C A Patil
Cell blocks and fine needle aspirations can be used for cytopathological diagnosis. Conventional fine needle aspiration smears provide limited material for diagnosis. The cell block technique provides more tissue, which improves diagnostic accuracy. We compared a modified cell block cytology to fine needle aspiration for providing optimal preservation of histochemical and immunocytochemical properties
-
Copy number variation, gene expression and histological localization of human beta-defensin 2 in patients with adeno-tonsillar hypertrophy. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-18 Fulvio Celsi,Luisa Zupin,Emmanouil Athanasakis,Eva Orzan,Domenico Leonardo Grasso,Sergio Crovella
ABSTRACT Both bacterial infections and innate oral immunity response participate in development of adeno-tonsillar hypertrophy (ATH). ATH can lead to obstructive sleep apnea. We investigated the beta-defensin 2 (hBD-2) encoding gene, DEFB4, by analyzing the copy number variations (CNVs) of the defensin gene cluster in patients with ATH and by correlating CNV with DEFB4 gene expression. We enrolled
-
Effects of cannabinoid receptor 2 synthetic agonist, AM1241, on bleomycin induced pulmonary fibrosis Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-17 Ali Parlar; Seyfullah Oktay Arslan; Onder Yumrutas; Ebru Elibol; Alper Yalcin; Fatih Uckardes; Hasan Aydin; Muhammed Fatih Dogan; Elif Kayhan Kustepe; Mehmet Kaya Ozer
ABSTRACT Bleomycin (BLM) is a chemotherapeutic agent that can cause pulmonary fibrosis. Little is known about the possible protective role of the CB2 receptor agonist, AM1241. We investigated the effects of CB2 receptor activation by AM1241 on BLM induced lung fibrosis in a rat model. BLM was administered via the trachea. Adult female Wistar rats were divided into five groups: saline (control group)
-
Does melamine exposure during infancy cause rhabdomyolysis? Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-12 Zuleyha Erisgin,Murat Usta
ABSTRACT We investigated the possible toxic effects of melamine on muscle tissue in rats using biochemical, hematological and histopathological methods. We used three groups of female albino Wistar rats. The first group was given 0.1 ml saline. The second and third groups were given 50 and 75 mg/kg melamine dissolved in 0.1 ml saline, respectively, daily for 21 days. On day 45, all rats were sacrificed
-
Fast acetone tissue processing of human organs provides tissue characteristics equal to conventional processing. Biotech. Histochem. (IF 1.203) Pub Date : 2020-06-12 R García-Garza,D Salas-Treviño,F A Soto-Patiño,E A González-Murillo,E E Castelán-Maldonado,O Saucedo-Cárdenas,V J Romero-Díaz,S E Velázquez-Gauna,A Soto-Domínguez
ABSTRACT Routine preparation of paraffin embedded tissue for histopathological diagnosis, here termed conventional histological technique (CT), whether performed manually or using an automated system, requires approximately 12 h. We developed earlier a rapid acetone dehydration technique (AT) for processing biopsies of nervous tissue that meets requirements for preserving tissue morphology and staining
Contents have been reproduced by permission of the publishers.