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A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure Biotech. Histochem. (IF 1.6) Pub Date : 2024-03-14 Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak
Myoglobinuric acute renal failure (MARF) is a structural and functional disorder that occurs in the kidney following the release of muscle cell contents into the circulation. In this present study,...
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Diazoxide attenuates DOX-induced cardiotoxicity in cultured rat myocytes Biotech. Histochem. (IF 1.6) Pub Date : 2024-03-05 Celal Guven, Eylem Taskin, Özgül Aydın, Salih Tunç Kaya, Yusuf Sevgiler
Doxorubicin (DOX)-induced cardiotoxicity is a well known clinical problem, and many investigations have been made of its possible amelioration. We have investigated whether diazoxide (DIA), an agon...
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Effects of an energy drink on myonectin in the liver, kidney and skeletal muscle of exercised rats Biotech. Histochem. (IF 1.6) Pub Date : 2024-01-31 Emsal Cagla Avcu, Vedat Çınar, Yavuz Yasul, Taner Akbulut, Zarife Pancar, İ̇sa Aydemir, Suna Aydin, Mehmet Hanifi Yalcin, Suleyman Aydin
Myonectin is a hormone that is produced mainly by skeletal muscle. We investigated the effects of exercise and energy drink (ED) administration on myonectin expression in skeletal muscle, liver and...
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Role of asprosin and meteorin-like peptide in progression of actinic keratosis to squamous cell carcinoma Biotech. Histochem. (IF 1.6) Pub Date : 2024-01-09 Esma Inan Yuksel, Demet Cicek, Betul Demir, Nevin Kocaman, Ilknur Calik, Tuncay Kuloglu
Squamous cell carcinoma (SCC) often develops from an underlying premalignant lesion. Factors that affect the progression of actinic keratosis (AK) to invasive SCC are not fully known. Asprosin (ASP...
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Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice Biotech. Histochem. (IF 1.6) Pub Date : 2023-11-29 Hao Hao, Youmei Xu, Rui Chen, Shanshan Qi, Xiang Liu, Beibei Lin, Xiaohua Chen, Xiaoying Zhang, Lijuan Yue, Chen Chen
We investigated possible protective effects of chlorogenic acid (CGA) against cyclophosphamide (CP) induced hepatic injury in mice. We measured aminotransferase alanine transaminase (ALT) and aspar...
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Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model Biotech. Histochem. (IF 1.6) Pub Date : 2023-11-07 Suna Aydin, Faruk Kilinc, Kader Ugur, Mustafa Ata Aydin, Mehmet Hanifi Yalcin, Tuncay Kuloglu, Nalan Kaya Tektemur, Serdal Albayrak, Elif Emre, Meltem Yardim, Ramazan Fazil Akkoc, Serhat Hancer, İbrahim Sahin, Vedat Cinar, Taner Akbulut, Selcuk Demircan, Bahri Evren, Berrin Tarakci Gencer, Aziz Aksoy, Merve Yilmaz Bozoglan, İsa Aydemir, Suleyman Aydin
Metabolic syndrome (MetS) is a prevalent public health problem. Uric acid (UA) is increased by MetS. We investigated whether administration of UA and 10% fructose (F) would accelerate MetS formatio...
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Using extract from alkanet (Alkanna tinctoria) as a source of both a red lipid stain and a blue counterstain for histology Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Hayfaa A. Alshamar, Richard W. Dapson
Alkanet (Alkanna tinctoria) is a plant native to and cultivated in parts of Europe, Asia and the Middle East. It has been used for thousands of years as a medicinal agent and as a colorant for text...
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Pravastatin attenuates isoprenaline induced cardiac fibrosis in a mouse model Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Abhinav Rana, Thakur Uttam Singh, Meemansha Sharma, Manju Gari, Tarun Kumar, Subhashree Parida, Madhu Cholenahalli Lingaraju, Asok Kumar Mariappan, Akhilesh Kumar, Dinesh Kumar
We investigated the effects of pravastatin (PRAVA) on isoprenaline (ISP) induced cardiac fibrosis using four groups of mice: untreated control, PRAVA, ISP, ISP + PRAVA groups. ISP, 20 mg/kg, was ad...
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Disulfiram ameliorates bleomycin induced pulmonary inflammation and fibrosis in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Negar Hamidi, Farideh Feizi, Abbas Azadmehr, Ebrahim Zabihi, Soraya Khafri, Zeinab Zarei-Behjani, Zahra Babazadeh
Bleomycin (BL) is a widely used anticancer drug that can cause pulmonary fibrosis due to increased fibroblast proliferation and increased secretion of extracellular matrix. RASSF1A is a tumor suppr...
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Classification and naming of polymethine dyes used as staining agents for microscopy. A short guide for biomedical investigators Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Heinz Mustroph, Richard W. Horobin
The scientific literature contains many accounts of application of polymethine dyes, including cyanine dyes, as imaging agents, i.e., “biological stains,” for microscopic investigation of biologica...
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Role of canagliflozin in ameliorating isoprenaline induced cardiomyocyte oxidative stress via the heme oxygenase-1 mediated pathway Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Ahmed Ahmed, Dina Abdel-Rahman, Ehab M. Hantash
Canagliflozin (CZ) is commonly prescribed for management of type-2 diabetes mellitus (T2DM); it also can reduce the risk of myocardial infarction. We used 80 albino Wistar rats to investigate the c...
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Immunohistochemical evaluation of hormones secreted by pancreatic endocrine tumors Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31 Anne Mones, Sheila Criswell
The endocrine component of the pancreas is located primarily in the islets of Langerhans, but is also found as single cells among the acinar cells and duct epithelium. It currently is thought that ...
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-10-31
Published in Biotechnic & Histochemistry (Vol. 98, No. 8, 2023)
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-09-28
Published in Biotechnic & Histochemistry (Vol. 98, No. 7, 2023)
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Selenium protection from DNA damage and regulation of apoptosis signaling following cyclophosphamide induced cardiotoxicity in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-09-11 Emrah İpek, Mehmet Hesapçıoğlu, Mehmet Karaboğa, Hamdi Avcı
We investigated the mechanism of the cardioprotective effect of selenium (Se) against cyclophosphamide (CPA) induced cardiotoxicity in rats. We divided 24 female Wistar albino rats into four groups...
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Effects of adriamycin on cell differentiation and proliferation in rat testis Biotech. Histochem. (IF 1.6) Pub Date : 2023-09-01 Ali Tugrul Akin, Ayse Toluk, Saim Ozdamar, Serpil Taheri, Emin Kaymak, Ecmel Mehmetbeyoglu
Although adriamycin (ADR) is used to treat many cancers, it can be toxic to healthy organs including the testis. We investigated the effects of ADR on pluripotency in rat testis. Testicular damage ...
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Use of hyaluronic acid matrix in dorsal augmentation rhinoplasty Biotech. Histochem. (IF 1.6) Pub Date : 2023-08-30 Zuleyha Erisgin, Omer Hizli, Guven Yildirim, Cengiz Sivrikaya, Ahmet Burcin Sarisoy, Yonca Avci, Kursat Murat Ozcan
Augmentation rhinoplasty sometimes is required for patients with saddle nose deformity caused by failed rhinoplasty or facial trauma; finding appropriate grafting material remains a significant pro...
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Cocaine and amphetamine regulated transcript (CART): a newly characterized neuropeptide in human prostate Biotech. Histochem. (IF 1.6) Pub Date : 2023-08-24 Cyprian Weaver, Marie Antony, Jack Fite, Paari Murugan, Andrew C. Nelson, Juan C. Manivel
Cocaine and amphetamine regulated transcript (CART) is a somatostatin-like polypeptide. CART has been localized in the CNS, hypothalamo-pituitary-adrenocortical (HPA) axis, pancreatic islets and en...
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Effects of PKCε knockdown on mitochondrial membrane potential of human glioma cells in vitro and growth of U251 cell-derived tumors in vivo Biotech. Histochem. (IF 1.6) Pub Date : 2023-07-28 Yaming Xu, Jie Tao, Yafei Hou, Shuzhang Zhang
Glioma is the most common type of primary brain tumor; it exhibits great invasive capacity, morbidity and mortality. Protein kinase Cε (PKCε), a serine/threonine kinase, contributes to the developm...
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Influence of type 2 diabetes on immunohistochemical detection of TRAF6, cFos and NFATC1 in the gingiva in cases of chronic periodontitis Biotech. Histochem. (IF 1.6) Pub Date : 2023-07-24 R. Mathangi, Vidyarani Shyamsundar, A. Meenakshi, N. Aravindha Babu, Yaongamphi Vashum, S. Shila
Type 2 diabetes (T2D) and chronic periodontitis (CP) are common diseases worldwide. Although T2D increases the severity of CP and alveolar bone loss, the mechanism of this is not well understood. W...
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Effects of nicorandil on QT prolongation and myocardial damage caused by citalopram in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-07-19 Gozde Akturk, Serap Cilaker Micili, Ozlem Gursoy Doruk, Nil Hocaoglu, Pinar Akan, Bekir Ugur Ergur, Samar Ahmed, Sule Kalkan
Citalopram is a selective serotonin re-uptake inhibitor (SSRI) antidepressant; it exhibits the greatest cardiotoxic effect among SSRIs. Citalopram can cause drug-induced long QT syndrome (LQTS) and...
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An immunohistochemical study of the vasculature and stem cells and carbonic anhydrase IV in the ducts of irradiated and normal human submandibular salivary glands Biotech. Histochem. (IF 1.6) Pub Date : 2023-07-07 Robert S. Redman
ABSTRACT Therapeutic irradiation for cancers of the head and neck causes serious and irreversible damage to the salivary glands; the resulting adverse effects on salivary quality and quantity produce detrimental effects on teeth and oral mucosa. The salivary effects have been related mostly to loss of serous acini; damage to the ducts is relatively minor. Other radiation effects include fibrosis, adiposis
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Protective effect of Arum maculatum against dextran sulfate sodium induced colitis in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-07-03 Gülsüm Toparlı Doğan, Remziye Aysun Kepekçi, Nuray Bostancıeri, Mehmet Tarakçıoğlu
Ulcerative colitis (UC) is an inflammatory disease of the large intestine that is characterized by diarrhea, bloody stools, abdominal pain and mucosal ulceration. UC is treated with nonsteroidal an...
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KLF2 regulates stemness of human mesenchymal stem cells by targeting FGFR3 Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-28 Zhiyuan Gong, Zhanhao Shu, Ying Zhou, Yin Chen, Huiyong Zhu
Mesenchymal stem cells (MSCs) are an attractive source of pluripotent cells for regenerative therapy; however, maintaining stemness and self-renewal of MSCs during expansion ex vivo is challenging....
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Protective effect of ursodeoxycholic acid and resveratrol against tacrolimus induced hepatotoxicity Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-28 Suleyman Koc, Ahmet Aktas, Bilal Sahin, Hatice Ozer, Gozde Erturk Zararsiz
Tacrolimus (TAC) is a potent and well-tolerated immunosuppressive drug, but serious side effects including nephrotoxicity and hepatotoxicity have been reported. Ursodeoxycholic acid (UDCA) and resv...
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A filtration medium replacement method that increases the efficiency of immunofluorescence staining of oocytes Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-28 Bo Yao, Feng Liu, Xiaolong Mo, Qiongyou Liu, Yanping Ren
Immunofluorescence staining is used to investigate proteins and protein interactions in oocytes. In typical protocols, the medium that suspends the oocytes requires replacement more than ten times ...
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Protective effects of scutellarin in experimental colitis in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-20 Hasan Aksit, Dilek Aksit, Eren Altun
ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease of unknown etiology for which effective treatments are lacking. Scutellarin is a flavonoid with anti-apoptotic, antioxidant and anti-inflammatory effects. We investigated the possible protective effects of scutellarin on UC induced by acetic acid in rats. We used five groups of male rats: control, scutellarin, UC, UC + scutellarin, UC
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Effects of Polygonum cognatum Meissn. extract on indomethacin induced gastric damage in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-09 Yasin Bayir, Nermin Tagiyeva, Abdulmecit Albayrak, Abbas Ismayilov, Erol Akpinar, Erdem Toktay, Esen Sezen Karaoglan, Kushver Memmedova, Zekai Halici, Nilufar Rustemova
ABSTRACT We investigated the anti-ulcer activity of ethanol extracts of Polygonum cognatum on indomethacin induced gastric damage in rats. We evaluated the number of ulcer areas, oxidant and antioxidant parameters as well as histopathologic features in rat stomach. We measured the total antioxidant status of P. cognatum in concentrations from 1.56–100 mg/ml. P. cognatum extract inhibited indomethacin
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Silymarin protects against doxorubicin induced cardiotoxicity by down-regulating topoisomerase IIβ expression in mice Biotech. Histochem. (IF 1.6) Pub Date : 2023-06-05 Emrah İpek, Recai Tunca
ABSTRACT We investigated the potential protective effects of silymarin (SLY) on doxorubicin (DOX) induced chronic cardiotoxicity in mice. We used 30 male BALB/c mice assigned randomly to four experimental groups: control group administered normal saline orally daily and intraperitoneally (i.p.) twice/week during weeks 1, 2, 5 and 6; SLY group (was administered 100 mg/kg SLY daily by oral gavage for
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Modeling the extent of conjugation in histochemical dyes and fluorescent probes: clarification in calculating conjugated bond number (CBN) and introduction of a new parameter, resonance energy Biotech. Histochem. (IF 1.6) Pub Date : 2023-05-25 Richard W. Dapson, Richard W. Horobin
ABSTRACT Determining the extent of conjugation in dyes and fluorochromes is a helpful tool for understanding or predicting the behavior of these compounds when used as stains for microscopy. One measure that has been used repeatedly is conjugated bond number (CBN), which is the number of bonds in a conjugated system. CBN can be obtained by inspection of the structure of a compound, but the rules for
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Evaluation of DNA damage in obese children using the chromatin dispersion test Biotech. Histochem. (IF 1.6) Pub Date : 2023-05-23 Martha I. Dávila-Rodríguez, Francisco González-Salazar, Manuel López-Cabanillas, Ricardo M. Cerda-Flores, Elva I. Cortés-Gutiérrez
ABSTRACT Childhood obesity predicts adult obesity and may increase the lifetime risk of adverse health outcomes. Obesity is characterized by oxidative stress that can induce DNA damage; however, studies of childhood and adolescent obesity are scarce. We investigated DNA damage due to obesity in Mexican children using the chromatin dispersion test (CDT). We evaluated DNA damage to peripheral lymphocytes
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Role of p38 MAPK, Akt and NFκB in renoprotective effects of nebivolol on renal ischemia-reperfusion injury in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-05-22 Zahide Cavdar, Ayse Kocak, Cemre Ural, Aysan Afagh, Sibel Ersan, Seda Ozbal, Merve Tatli, Asli Celik, Sevki Arslan, Caner Cavdar
ABSTRACT Renal ischemia-reperfusion (I-R) injury is a complex pathophysiologic condition characterized by oxidative stress, inflammation and apoptosis. We investigated the potential renoprotective effect of nebivolol, a β1 adrenergic receptor blocker, against renal I-R injury. We focused on the role of nebivolol in activating p38 mitogen-activated protein kinase (MAPK) signaling, Akt (protein kinase
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Charles T. Willis April 17, 1929–December 17, 2022 Biotech. Histochem. (IF 1.6) Pub Date : 2023-05-11 Chad Fagan
Published in Biotechnic & Histochemistry (Vol. 98, No. 4, 2023)
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A tribute to Charles (Chuck) Willis Biotech. Histochem. (IF 1.6) Pub Date : 2023-05-11 David Penney
Published in Biotechnic & Histochemistry (Vol. 98, No. 4, 2023)
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Transmission electron microscopy assessment of a novel method for isolating pure exosomes from serum Biotech. Histochem. (IF 1.6) Pub Date : 2023-04-26 Jin Wen, Zhijun Zhang, Gang Feng, Yang Zhang, Hanzhong Li, Claude Lambert, Nora Mallouk, Guorong Li
ABSTRACT Serum exosomes frequently are used for liquid biopsy. Serum exosomes normally are isolated using ultracentrifugation; however, ultracentrifugation is time-consuming, labor intensive and requires a high-speed centrifuge. Many commercial kits use a precipitation-based method; however, this process can result in substantial contamination. We developed a new method to isolate pure serum exosomes
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Tribute to Chuck Willis Biotech. Histochem. (IF 1.6) Pub Date : 2023-04-25 Richard Dapson
Published in Biotechnic & Histochemistry (Vol. 98, No. 4, 2023)
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Effect of melatonin on low and high dose radiotherapy induced thyroid injury Biotech. Histochem. (IF 1.6) Pub Date : 2023-04-19 Serhat Aras, İ̇hsan Oğuz Tanzer, Seyhan Karaçavuş, Neslihan Sayir, Esra Erdem, Fatih Hacımustafaoğlu, Ceren Ezgi Erdoğan, Tansel Sapmaz, Türkan İkizceli, Halime Hanım Pençe, Kürşad Nuri Baydili, Tolga Katmer
ABSTRACT We investigated the radioprotective effect of melatonin (MEL) against thyroid gland damage in rats caused by flattening filter (FF) and flattening filter free (FFF) single dose X-ray beams. We used 48 female rats divided into six groups of eight: group 1, untreated control group; group 2, MEL treated group; group 3, FF-low dose rate radiotherapy (FF-LDR) group; group 4, FF-LDR + MEL group;
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Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult Biotech. Histochem. (IF 1.6) Pub Date : 2023-04-06 Flávia R. M. da Silva, Joyce R. Zapaterini, Tony F. Grassi, Lucas T. Bidinotto, Ana Angélica H. Fernandes, Luis F. Barbisan
ABSTRACT We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group
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RAS gene mutations and histomorphometric measurements in oral squamous cell carcinoma Biotech. Histochem. (IF 1.6) Pub Date : 2023-04-04 Tazeen Mustansar, Talat Mirza, Mushtaq Hussain
ABSTRACT Members of the RAS gene family frequently are mutated in cancers including oral squamous cell carcinoma (OSCC). We investigated the correlation of histological characteristics of OSCC with RAS gene mutations. We graded tumors and extracted genomic DNA from OSCC. The first two exons of KRAS, HRAS and NRAS genes were subjected to PCR amplification and DNA sequencing followed by bioinformatic
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Synergistic apoptotic effects of ethanolic extracts of ginger and Ganoderma lucidum in a colorectal cancer cell line Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-27 Amir Mohammad Saeedifar, Ali Ghazavi, Ghasem Mosayebi, Ali Ganji
ABSTRACT Current conventional therapy for colorectal cancer includes surgery, radiation and chemotherapy, all of which produce side effects. Herbal medicine can control the side effects of conventional treatments. We investigated the synergistic effect of a mixture of Zingiber officinale Roscoe (Ginger) and Ganoderma lucidum extracts on colorectal cancer cell apoptosis in vitro. We prepared ethanolic
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Effects of noopept on ocular, pancreatic and renal histopathology in streptozotocin induced prepubertal diabetic rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-22 Perihan Gurbuz, Halil Duzova, Asli Cetin Taslidere, Cemile Ceren Gul
ABSTRACT Diabetes mellitus (DM) is a chronic disease at all ages including childhood and puberty. Failure to treat DM can cause retinopathy, nephropathy and neuropathy. Endocrine and metabolic changes during the pubertal period complicate management of DM. Noopept is a cognitive enhancer that exhibits antidiabetic properties. We investigated the effect of noopept on the histopathology of the cornea
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Angiotensin II type 2 receptor agonist treatment of doxorubicin induced heart failure Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-20 Necip Ermis, Zeynep Ulutas, Onural Ozhan, Azibe Yildiz, Nigar Vardi, Cemil Colak, Hakan Parlakpinar
ABSTRACT Doxorubicin (DOX) is an anthracycline derivative used for treatment of malignancies; however, its clinical use is limited by its cardiotoxicity. We investigated the effects of angiotensin II type 2 receptor agonist compound 21 (C21) on DOX induced heart failure in rat heart. We compared C21 with losartan (LOS), an AT 1 receptor antagonist used for treating heart failure. We allocated 40 rats
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Carvacrol protects rat liver exposed to formaldehyde by regulating oxidative stress, and asprosin and subfatin hormones Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-13 E. Emre, M. Ogeturk, S. Aydın, T. Kuloglu, F. Aksu, A. Kavakli
ABSTRACT Toxic doses of formaldehyde (FA) can cause oxidative damage and impair energy metabolism. Asprosin (ASP) and subfatin (SUB) are adipokines produced by adipose tissue that help regulate energy metabolism. We investigated the effects of carvacrol (CAR), an antioxidant with hepatoprotective properties, on ASP and SUB in rats exposed to FA using immunohistochemistry and biochemistry. We used 42
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A fluorescence method to detect cyanophycin in the symbiotic cyanobacterium, Anabaena azollae Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-07 Ana L. Pereira, Francisco Carrapiço
ABSTRACT The cyanophycin content of the heterocystous nitrogen-fixing symbiotic cyanobacterium, Anabaena azollae, which inhabits an ovoid cavity in the dorsal leaf lobes of the fern, Azolla filiculoides, is seldom analyzed. To study the cyanophycin content in vegetative cells and heterocysts of A. azollae, we used three fluorochromes: aluminum trichloride, lead citrate and Wilson citroboric solution
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Correction Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-01
Published in Biotechnic & Histochemistry (Vol. 98, No. 5, 2023)
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-03-01
Published in Biotechnic & Histochemistry (Vol. 98, No. 6, 2023)
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Use of AgNOR staining to determine the effect of metoclopramide on neural tube development in early chick embryos Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-27 Abdulkadir Bilir, Esra Aslan, Erdal Horata, Hilal Guzel, Emre Atay, Ozan Turamanlar, Tolga Ertekin
ABSTRACT Nausea and vomiting during pregnancy are common problems and prolonged pharmacological treatment often is needed; however, the teratogenic effects of anti-emetic drugs on neural tube (NT) development are not clear. We investigated the effects of different doses of metoclopramide on NT development in 48 and 72 h chick embryos using an argyrophilic nucleolar organizing region (AgNOR) staining
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Betatrophin, elabela, asprosin, glucagon and subfatin peptides in breast tissue, blood and milk in gestational diabetes Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-24 Adem Yavuz, Mustafa Ata Aydin, Kader Ugur, Suna Aydin, Arzu Senol, Yakup Baykus, Rulin Deniz, İbrahim Sahin, Mehmet Hanifi Yalcin, Berrin Tarakci Gencer, Yaprak Kandemir Deniz, Sefer Ustebay, Zuhal Karaca Karagoz, Elif Emre, Suleyman Aydin
ABSTRACT We investigated the presence of asprosin (ASP), betatrophin, elabela (ELA), glucagon and subfatin (SUB) in the milk of mothers with gestational diabetes mellitus (GDM) and compared their levels with blood levels. We also investigated whether these peptides are synthesized by the breast. We investigated 12 volunteer mothers with GDM and 14 pregnant non-GDM control mothers. The peptides were
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Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-23 Ozlem Ozden Akkaya, Tayfun Dikmen, Shah Nawaz, ASM Golam Kibria, Korhan Altunbaş, Artay Yağci, Metin Erdoğan, Mustafa Volkan Yaprakci
ABSTRACT Bone marrow derived stem cells (BMSC) are the most utilized cell type in the field of bone regeneration. Although BMSC are both safe and efficacious, the search for alternative sources for stem cells continues. We investigated bovine BMSC and adipose tissue derived mesenchymal stem cells (ATSC) using immunofluorescence and PCR. We further compared the osteogenic differentiation potentials
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Inula viscosa ameliorates acetic acid induced ulcerative colitis in rats Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-22 Mustafa Cellat, İbrahim Ozan Tekeli, Erdinç Türk, Tuba Aydin, Ahmet Uyar, Cafer Tayer İşler, İshak Gökçek, Muhammed Etyemez, Mehmet Güvenç
ABSTRACT Increased pro-inflammatory cytokines and oxidative stress contribute to the pathophysiology of ulcerative colitis (UC). Inula viscosa is a plant with antioxidant and anti-inflammatory properties. We investigated the effect of an ethanolic extract of I. viscosa on an experimental UC model created using acetic acid. Rats were divided into four groups of eight: group 1, control; group 2, 3% acetic
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Seed coating with micronutrients improves germination, growth, yield and microelement nutrients of maize (Zea mays L.) Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-22 Fa-Bo Chen, Yun-Chao Feng, Shi-Ping Huo
ABSTRACT Soil and foliar application are the most widely used methods for adding micronutrients to maize. High quality micronutrient fertilizers, however, are difficult to obtain in developing countries; micronutrient seed coatings are an attractive and practical alternative. We applied this approach to maize (Zea mays L.) to demonstrate the effects of boron (B), iron (Fe), manganese (Mn), molybdenum
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Identifying stem cells in the main excretory ducts of rat major salivary glands: adventures with commercial antibodies Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-13 Robert S. Redman, José C. Alvarez-Martinez
ABSTRACT We investigated the entire length of the main excretory ducts (MED) of the major sublingual, parotid and submandibular salivary glands of mature laboratory rats for mucous (goblet) and luminal ciliated cells, biomarkers of cell proliferation, apoptosis, and five biomarkers of stem cells. Spleen and testis were used as positive controls. We used formalin fixed, paraffin embedded tissues. No
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Effects of silibinin on apoptosis and insulin secretion in rat RINm5F pancreatic β-cells Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-10 Maryam Mazraesefidi, Mehdi Mahmoodi, Mohammadreza Hajizadeh
ABSTRACT We investigated whether silibinin, a flavonoid, might be useful for treating diabetes mellitus by treating five groups of rat RINm5F β-insulinemia cells as follows: control streptozotocin (STZ) group administered citrate buffer and dimethyl sulfoxide; STZ group administered 20 mM STZ; silibinin group administered 50 µM silibinin; pre-silibinin group administered 50 µM silibinin 5 h before
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Quercetin ameliorates acute acrylamide induced spleen injury Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-08 Piyush Shukla, Naresh Kumar Sahu, Raj Kumar, Deep Kaur Dhalla, Samrat Rakshit, Monika Bhadauria, Narottam Das Agrawal, Sadhana Shrivastava, Sangeeta Shukla, Satendra Kumar Nirala
ABSTRACT Acrylamide is used for industrial and laboratory purposes; it also is produced during cooking of carbohydrate-rich food at high temperature. We investigated the therapeutic potential of quercetin for treatment of acute acrylamide induced injury to the spleen. We used female albino rats treated with acrylamide for 10 days followed by oral administration of quercetin in three doses for 5 days
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-08
Published in Biotechnic & Histochemistry (Vol. 98, No. 5, 2023)
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-07
Published in Biotechnic & Histochemistry (Vol. 98, No. 3, 2023)
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-07
Published in Biotechnic & Histochemistry (Vol. 98, No. 4, 2023)
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Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-06 Salih Tunc Kaya
ABSTRACT I investigated the effects of diazoxide, a mitochondrial potassium channel opener, on streptozotocin (STZ) induced pancreatic β cell damage via the HSP70/HSP90/TLR4/AMPK signaling pathways in vitro. I used the pancreatic β cell line, 1.1B4, to create four groups: control, STZ treated, diazoxide treated, STZ + diazoxide treated. The STZ treated cells were exposed to 20 µM STZ for 2 h with or
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Stains recently certified Biotech. Histochem. (IF 1.6) Pub Date : 2023-02-01
Published in Biotechnic & Histochemistry (Vol. 98, No. 2, 2023)
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Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab Biotech. Histochem. (IF 1.6) Pub Date : 2022-12-09 Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman
ABSTRACT Bevacizumab is a recombinant humanized monoclonal antibody whose adverse effects include cardiotoxicity. We investigated whether using adenosine triphosphate (ATP) or benidipine either separately or together protects against cardiac damage induced by bevacizumab in rats. Forty Wistar albino male rats were allocated to five groups of eight: bevacizumab (Bv), ATP + bevacizumab (ABv), benidipine + bevacizumab