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Engineered Aptamers for Enhanced COVID-19 Theranostics Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2021-01-15 Caleb Acquah, Jaison Jeevanandam, Kei Xian Tan, Michael K. Danquah
Introduction The 2019-novel coronavirus disease (COVID-19) is an intractable global health challenge resulting in an aberrant rate of morbidity and mortality worldwide. The mode of entry for SARS-CoV-2 into host cells occurs through clathrin-mediated endocytosis. As part of the efforts to mitigate COVID-19 infections, rapid and accurate detection methods, as well as smart vaccine and drug designs with
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Correction to: A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-11-30 Rachel R. Besser, Annie C. Bowles, Ahmad Alassaf, Daniel Carbonero, Renata Maciel, Mario Saporta, Ashutosh Agarwal
This Erratum is to add the 2nd affiliation for Ahmad Alassaf.
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General Characteristics of Microbubble-Adenovirus Vectors Carrying Genes Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-11-30 Lingjie Yang, Juan Ma, Lina Guan, Yuming Mu
Introduction Transferring genes safely, targeting cells and achieving efficient transfection are urgent problems in gene therapy that need to be solved. Combining microbubbles (MBs) and viruses to construct double vectors has become a promising approach for gene delivery. Understanding the characteristic performance of MBs that carry genes is key to promoting effective gene transfer. Therefore, in
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Interstitial Hypertension Suppresses Escape of Human Breast Tumor Cells Via Convection of Interstitial Fluid Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-11-09 Joe Tien, Yoseph W. Dance, Usman Ghani, Alex J. Seibel, Celeste M. Nelson
Introduction Interstitial hypertension, a rise in interstitial fluid pressure, is a common feature of many solid tumors as they progress to an invasive state. It is currently unclear whether this elevated pressure alters the probability that tumor cells eventually escape into a neighboring blood or lymphatic vessel. Methods In this study, we analyze the escape of MDA-MB-231 human breast tumor cells
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Cutting and Bonding Parafilm ® to Fast Prototyping Flexible Hanging Drop Chips for 3D Spheroid Cultures Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-27 Jing Jing Fu, Xiao Hui Lv, Lin Xiang Wang, Xiu He, Yuan Li, Ling Yu, Chang Ming Li
A fast and low-cost fabrication process of flexible hanging drop chips for 3D spheroid cultures was proposed by cutting and bonding Parafilm®, a cohesive thermoplastic. The Parafilm® Hanging Drop Chip (PHDC) was assembled by two-layer of Parafilm® sheet with different sizes of holes. The hole on the upper layer of the Parafilm® is smaller than the hole on the bottom layer. The impact of hole size and
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Investigating the Interaction Between Circulating Tumor Cells and Local Hydrodynamics via Experiment and Simulations Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-21 Marianna Pepona, Peter Balogh, Daniel F. Puleri, William F. Hynes, Claire Robertson, Karen Dubbin, Javier Alvarado, Monica L. Moya, Amanda Randles
Introduction The biological and mechanical properties of circulating tumor cells (CTCs) in combination with the hemodynamics affect the preference of metastatic sites in the vasculature. Despite the extensive literature on the effects of biological properties on cell adhesion, the effects of hydrodynamic forces on primary attachment remains an active area of research. Using simulations in conjunction
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Development of Coagulation Factor XII Antibodies for Inhibiting Vascular Device-Related Thrombosis Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-13 T. C. L. Kohs, C. U. Lorentz, J. Johnson, C. Puy, S. R. Olson, J. J. Shatzel, D. Gailani, M. T. Hinds, E. I. Tucker, A. Gruber, O. J. T. McCarty, M. Wallisch
Introduction Vascular devices such as stents, hemodialyzers, and membrane oxygenators can activate blood coagulation and often require the use of systemic anticoagulants to selectively prevent intravascular thrombotic/embolic events or extracorporeal device failure. Coagulation factor (F)XII of the contact activation system has been shown to play an important role in initiating vascular device surface-initiated
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Immune Cell Paracrine Signaling Drives the Neutrophil Response to A. fumigatus in an Infection-on-a-Chip Model Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-13 Laurel E. Hind, Morgan A. Giese, Taylor J. Schoen, David J. Beebe, Nancy Keller, Anna Huttenlocher
Introduction Neutrophils act as first responders during an infection, following signals from the pathogen as well as other host cells to migrate from blood vessels to the site of infection. This tightly regulated process is critical for pathogen clearance and, in many cases, eliminates the pathogen without the need for an additional immune response. It is, therefore, critical to understand what signals
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Structure-Based Design of Novel Peptidomimetics Targeting the SARS-CoV-2 Spike Protein Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-13 Manikandan Alagumuthu, Sajjan Rajpoot, Mirza S. Baig
Purpose SARS-CoV-2 is a SARS-like novel coronavirus strain first identified in December 2019 in Wuhan, China. The virus has since spread globally, resulting in the current ongoing coronavirus disease 19 (COVID-19) pandemic. SARS-CoV-2 spike protein is a critical factor in the COVID-19 pathogenesis via interactions with the host cell angiotensin-converting enzyme 2 (ACE2) PD domain. Worldwide, numerous
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Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-10-06 Sandeep Kadekar, Laurent Barbe, Martin Stoddart, Oommen P. Varghese, Maria Tenje, Gemma Mestres
Introduction Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The aim of this work was to evaluate the effect of 5-AzaC addition frequency on hMSCs priming (i.e., indicating
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Effect of Heat Level and Expose Time on Denaturation of Collagen Tissues Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-09-28 İrem Deniz Derman, Esat Can Şenel, Onur Ferhanoğlu, İnci Çilesiz, Murat Kazanci
Introduction The applied heat level and expose time are main issues in certain operations/applications, such as a laser assisted tissue welding, preparation of collagen-based biomaterials (films, implants). Therefore, the precise investigation of these parameters is crucial. The results can serve as a guideline to assess potential effects while maintaining the functionality of the collagen structures
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Lactate Exposure Promotes Immunosuppressive Phenotypes in Innate Immune Cells Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-09-21 Rapeepat Sangsuwan, Bhasirie Thuamsang, Noah Pacifici, Riley Allen, Hyunsoo Han, Svetlana Miakicheva, Jamal S. Lewis
Introduction Lactate secreted by tumors is not just a byproduct, but rather an active modulator of immune cells. There are few studies aimed at investigating the true effect of lactate, which is normally confounded by pH. Such a knowledge gap needs to be addressed. Herein, we studied the immunomodulatory effects of lactate on dendritic cells (DCs) and macrophages (MΦs). Methods Bone marrow-derived
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CaliPro : A Calibration Protocol That Utilizes Parameter Density Estimation to Explore Parameter Space and Calibrate Complex Biological Models Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-09-15 Louis R. Joslyn, Denise E. Kirschner, Jennifer J. Linderman
Introduction Mathematical and computational modeling have a long history of uncovering mechanisms and making predictions for biological systems. However, to create a model that can provide relevant quantitative predictions, models must first be calibrated by recapitulating existing biological datasets from that system. Current calibration approaches may not be appropriate for complex biological models
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CRISPR-Mediated Isogenic Cell-SELEX Approach for Generating Highly Specific Aptamers Against Native Membrane Proteins Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-09-11 Jonah C. Rosch, Emma H. Neal, Daniel A. Balikov, Mohsin Rahim, Ethan S. Lippmann
Introduction The generation of affinity reagents that bind native membrane proteins with high specificity remains challenging. Most in vitro selection paradigms utilize different cell types for positive and negative rounds of selection (where the positive selection is against a cell that expresses the desired membrane protein and the negative selection is against a cell that lacks the protein). However
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Dynamic Regulation of JAK-STAT Signaling Through the Prolactin Receptor Predicted by Computational Modeling Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-09-08 Ryland D. Mortlock, Senta K. Georgia, Stacey D. Finley
Introduction The expansion of insulin-producing beta cells during pregnancy is critical to maintain glucose homeostasis in the face of increasing insulin resistance. Prolactin receptor (PRLR) signaling is one of the primary mediators of beta cell expansion during pregnancy, and loss of PRLR signaling results in reduced beta cell mass and gestational diabetes. Harnessing the proliferative potential
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Potential Applications of Microfluidics to Acute Kidney Injury Associated with Viral Infection. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-31 Holly Ryan,Chelsey S Simmons
The kidneys are susceptible to adverse effects from many diseases, including several that are not tissue-specific. Acute kidney injury is a common complication of systemic diseases such as diabetes, lupus, and certain infections including the novel coronavirus (SARS-CoV-2). Microfluidic devices are an attractive option for disease modeling, offering the opportunity to utilize human cells, control experimental
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3D Microwell Platforms for Control of Single Cell 3D Geometry and Intracellular Organization Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-20 Robin E. Wilson, Aleksandra K. Denisin, Alexander R. Dunn, Beth L. Pruitt
Introduction Cell structure and migration is impacted by the mechanical properties and geometry of the cell adhesive environment. Most studies to date investigating the effects of 3D environments on cells have not controlled geometry at the single-cell level, making it difficult to understand the influence of 3D environmental cues on single cells. Here, we developed microwell platforms to investigate
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Scaffold Architecture and Matrix Strain Modulate Mesenchymal Cell and Microvascular Growth and Development in a Time Dependent Manner Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-18 Gennifer Chiou, Elysa Jui, Allison C. Rhea, Aparna Gorthi, Solaleh Miar, Francisca M. Acosta, Cynthia Perez, Yasir Suhail, Kshitiz, Yidong Chen, Joo L. Ong, Rena Bizios, Christopher Rathbone, Teja Guda
Background Volumetric tissue-engineered constructs are limited in development due to the dependence on well-formed vascular networks. Scaffold pore size and the mechanical properties of the matrix dictates cell attachment, proliferation and successive tissue morphogenesis. We hypothesize scaffold pore architecture also controls stromal-vessel interactions during morphogenesis. Methods The interaction
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A Theme Series on Emerging Technologies for Use in the Study, Diagnosis and Treatment of Patients with COVID-19. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-11 Chelsey S Simmons,Owen J T McCarty,Anubhav Tripathi
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Scalable COVID-19 Detection Enabled by Lab-on-Chip Biosensors. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-10 Carly Tymm,Junhu Zhou,Amogha Tadimety,Alison Burklund,John X J Zhang
IntroductionThe emergence of a novel coronavirus, SARS-CoV-2, has highlighted the need for rapid, accurate, and point-of-care diagnostic testing. As of now, there is not enough testing capacity in the world to meet the stated testing targets, which are expected to skyrocket globally for broader testing during reopening AimThis review focuses on the development of lab-on-chip biosensing platforms for
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Stromal Cell-Derived Factor-1a Autocrine/Paracrine Signaling Contributes to Spatiotemporal Gradients in the Brain Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-07 Kassondra N. Hickey, Shannon M. Grassi, Michael R. Caplan, Sarah E. Stabenfeldt
Introduction Stromal cell derived factor-1a (SDF-1a) and its receptor CXCR4 modulate stem cell recruitment to neural injury sites. SDF-1a gradients originating from injury sites contribute to chemotactic cellular recruitment. To capitalize on this injury-induced cell recruitment, further investigation of SDF-1a/CXCR4 signaling dynamics are warranted. Here, we studied how exogenous SDF-1a delivery strategies
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Supercharged eGFP-TRAIL Decorated NETs to Ensnare and Kill Disseminated Tumor Cells. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-06 Thong M Cao,Michael R King
BackgroundNETosis is an innate immune response elicited by activated neutrophils to fight microbial infections. Activated neutrophils release DNA fibers decorated with anti-microbial proteins called neutrophil extracellular traps (NETs) into the extracellular space to trap and kill surrounding microbes. MethodsHere, we show that tumor-derived IL-8 released by cancer cells also activates the release
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Design of a Microfluidic Bleeding Chip to Evaluate Antithrombotic Agents for Use in COVID-19 Patients. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-08-06 Hari Hara Sudhan Lakshmanan,Adity A Pore,Tia C L Kohs,Feyza Yazar,Rachel M Thompson,Patrick L Jurney,Jeevan Maddala,Sven R Olson,Joseph J Shatzel,Siva A Vanapalli,Owen J T McCarty
IntroductionInterventions that could prevent thrombosis, clinical decompensation, and respiratory compromise in patients with novel coronavirus disease (COVID-19) are key to decrease mortality rate. Studies show that profound cytokine release and excessive activation of blood coagulation appear to be key drivers of COVID-19 associated mortality. Since limited in vitro methods exist for assessing the
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Structure-Guided Molecular Engineering of a Vascular Endothelial Growth Factor Antagonist to Treat Retinal Diseases Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-31 Rakeeb Kureshi, Angela Zhu, Jikui Shen, Stephany Y. Tzeng, Leilani R. Astrab, Paul R. Sargunas, Jordan J. Green, Peter A. Campochiaro, Jamie B. Spangler
Background Ocular neovascularization is a hallmark of retinal diseases including neovascular age-related macular degeneration and diabetic retinopathy, two leading causes of blindness in adults. Neovascularization is driven by the interaction of soluble vascular endothelial growth factor (VEGF) ligands with transmembrane VEGF receptors (VEGFR), and inhibition of the VEGF pathway has shown tremendous
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Pre-existing Cell States Control Heterogeneity of Both EGFR and CXCR4 Signaling Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-27 Phillip C. Spinosa, Patrick C. Kinnunen, Brock A. Humphries, Gary D. Luker, Kathryn E. Luker, Jennifer J. Linderman
Introduction CXCR4 and epidermal growth factor receptor (EGFR) represent two major families of receptors, G-protein coupled receptors and receptor tyrosine kinases, with central functions in cancer. While utilizing different upstream signaling molecules, both CXCR4 and EGFR activate kinases ERK and Akt, although single-cell activation of these kinases is markedly heterogeneous. One hypothesis regarding
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Gelatin Promotes Cell Retention Within Decellularized Heart Extracellular Matrix Vasculature and Parenchyma Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-27 Karis R. Tang-Quan, Yutao Xi, Camila Hochman-Mendez, Qian Xiang, Po-Feng Lee, Luiz C. Sampaio, Doris A. Taylor
Introduction Recellularization of organ decellularized extracellular matrix (dECM) offers a potential solution for organ shortage in allograft transplantation. Cell retention rates have ranged from 10 to 54% in varying approaches for reseeding cells in whole organ dECM scaffolds. We aimed to improve recellularization by using soluble gelatin as a cell carrier to deliver endothelial cells to the coronary
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Animal Models to Study Emerging Technologies Against SARS-CoV-2. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-27 Jhinuk Basu Mullick,Chelsey S Simmons,Janak Gaire
New technologies are being developed toward the novel coronavirus SARS-CoV-2 to understand its pathogenesis and transmission, to develop therapeutics and vaccines, and to formulate preventive strategies. Animal models are indispensable to understand these processes and develop and test emerging technologies; however, the mechanism of infection for SARS-CoV-2 requires certain similarities to humans
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Significant Unresolved Questions and Opportunities for Bioengineering in Understanding and Treating COVID-19 Disease Progression. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-27 Jasmine Shirazi,Michael J Donzanti,Katherine M Nelson,Ryan Zurakowski,Catherine A Fromen,Jason P Gleghorn
COVID-19 is a disease that manifests itself in a multitude of ways across a wide range of tissues. Many factors are involved, and though impressive strides have been made in studying this novel disease in a very short time, there is still a great deal that is unknown about how the virus functions. Clinical data has been crucial for providing information on COVID-19 progression and determining risk
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Pathologic Shear and Elongation Rates Do Not Cause Cleavage of Von Willebrand Factor by ADAMTS13 in a Purified System. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-17 Maria Bortot,Alireza Sharifi,Katrina Ashworth,Faye Walker,Allaura Cox,Katherine Ruegg,Nathan Clendenen,Keith B Neeves,David Bark,Jorge Di Paola
IntroductionPathological flows in patients with severe aortic stenosis are associated with acquired von Willebrand syndrome. This syndrome is characterized by excessive cleavage of von Willebrand factor by its main protease, A Disintegrin and Metalloproteinase with a Thrombospondin Type 1 Motif, Member 13 (ADAMTS13) leading to decreased VWF function and mucocutaneous bleeding. Aortic valve replacement
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Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-14 Shangping Wang, Pei-Chih Lee, Amanda Elsayed, Fan Zhang, Yong Zhang, Pierre Comizzoli, Gloria D. Elliott
Introduction Maintaining a stable dry state is critical for long-term preservation of live biomaterials at suprazero temperatures. The objective of the study was to characterize the effect of moisture content on DNA integrity within the germinal vesicle (GV) of feline oocytes following dehydration and storage at 22–24 °C. Methods Using microwave-assisted drying, conditions that led to a predictable
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Towards an Insulin Resistant Adipose Model on a Chip Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-14 Nida Tanataweethum, Franklin Zhong, Allyson Trang, Chaeeun Lee, Ronald N. Cohen, Abhinav Bhushan
Introduction Adipose tissue and adipocytes are primary regulators of insulin sensitivity and energy homeostasis. Defects in insulin sensitivity of the adipocytes predispose the body to insulin resistance (IR) that could lead to diabetes. However, the mechanisms mediating adipocyte IR remain elusive, which emphasizes the need to develop experimental models that can validate the insulin signaling pathways
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Shear Stress Increases V–H $$^{+}$$ + -ATPase and Acidic Vesicle Number Density, and p-mTORC2 Activation in Prostate Cancer Cells Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-07-02 Zeina S. Khan, Fazle Hussain
Introduction Cells in the tumor microenvironment experience mechanical stresses, such as compression generated by uncontrolled cell growth within a tissue, increased substrate stiffness due to tumor cell extracellular matrix (ECM) remodeling, and leaky angiogenic vessels which involve low fluid shear stress. With our hypothesis that shear stress increases V–H\(^+\)-ATPase number density in prostate
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The Impact of COVID-19 on Cancer Risk and Treatment. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-29 Nidhi Jyotsana,Michael R King
Millions of people are being infected with COVID-19 around the globe. Though the majority of them will recover, cancer patients remain at a higher risk to SARS-CoV-2 infection and its related severe outcomes. Understanding how viruses contribute to human cancers provides us with new opportunities for preventing or treating virus-associated cancers. However, a limited amount of research has been done
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Tissue-Engineered Stromal Reticula to Study Lymph Node Fibroblastic Reticular Cells in Type I Diabetes Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-26 Freddy Gonzalez Badillo, Flavia Zisi Tegou, Riccardo Masina, Shane Wright, Mackenzie Scully, Laura Harwell, Michael Lupp, Jorge Postigo-Fernandez, Remi J. Creusot, Alice A. Tomei
Introduction Fibroblastic reticular cells (FRCs) support and remodel the lymph node (LN), express and present self-antigens to T cells to promote tolerance. In Type 1 diabetes (T1D), decrease in FRC frequency and in their expression of T1D-related self-antigens may hinder tolerogenic engagement of autoreactive T cells. FRC reticular organization in LNs is critical for adaptive immunity. Thus, we engineered
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Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-α Induced Injury Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-24 Sanjin Hosic, Will Lake, Eric Stas, Ryan Koppes, David T. Breault, Shashi K. Murthy, Abigail N. Koppes
Introduction The intestinal epithelium contains specialized cells including enterocytes, goblet, Paneth, enteroendocrine, and stem cells. Impaired barrier integrity in Inflammatory Bowel Disease is characterized by elevated levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Prior studies in immortalized lines such as Caco-2, without native epithelial heterogeneity
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Re-engineering Antimicrobial Peptides into Oncolytics Targeting Drug-Resistant Ovarian Cancers Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-24 Matthew R. Aronson, Erika S. Dahl, Jacob A. Halle, Andrew W. Simonson, Rose A. Gogal, Adam B. Glick, Katherine M. Aird, Scott H. Medina
Introduction Bacteria and cancer cells share a common trait—both possess an electronegative surface that distinguishes them from healthy mammalian counterparts. This opens opportunities to repurpose antimicrobial peptides (AMPs), which are cationic amphiphiles that kill bacteria by disrupting their anionic cell envelope, into anticancer peptides (ACPs). To test this assertion, we investigate the mechanisms
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Emerging Technologies for Use in the Study, Diagnosis, and Treatment of Patients with COVID-19. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-24 Maria Tsikala Vafea,Eleftheria Atalla,Joanna Georgakas,Fadi Shehadeh,Evangelia K Mylona,Markos Kalligeros,Eleftherios Mylonakis
IntroductionThe COVID-19 pandemic has caused an unprecedented health and economic worldwide crisis. Innovative solutions are imperative given limited resources and immediate need for medical supplies, healthcare support and treatments. AimThe purpose of this review is to summarize emerging technologies being implemented in the study, diagnosis, and treatment of COVID-19. ResultsKey focus areas include
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Shear-Induced Platelet Activation is Sensitive to Age and Calcium Availability: A Comparison of Adult and Cord Blood Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-22 Jawaad Sheriff, Lisa E. Malone, Cecilia Avila, Amanda Zigomalas, Danny Bluestein, Wadie F. Bahou
Introduction Antiplatelet therapy for neonates and infants is often extrapolated from the adult experience, based on limited observation of agonist-induced neonatal platelet hypoactivity and poor understanding of flow shear-mediated platelet activation. Therefore, thrombotic events due to device-associated disturbed flow are inadequately mitigated in critically ill neonates with indwelling umbilical
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SIRT1 Inhibits High Shear Stress-Induced Apoptosis in Rat Cortical Neurons Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-17 Wei Song, Mei-Li Liu, Zhi-Jun Zhao, Chong-Quan Huang, Jun-Wei Xu, An-Qing Wang, Ping Li, Yu-Bo Fan
Introduction Sirtuin1 (SIRT1), one of NAD+-dependent protein deacetylases, is proved to be neuroprotective in aging diseases, but its effect on neuronal apoptosis has not been clarified. To investigate the role of SIRT1 in inhibiting neuronal apoptosis, SIRT1 was interfered or overexpressed in cortical neurons. Methods We exerted overloading laminar shear stress with 10 dyn/cm2 for 4, 8, and 12 h on
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Organoids as Complex In Vitro Models for Studying Radiation-Induced Cell Recruitment. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-15 Benjamin C Hacker,Marjan Rafat
Patients with triple negative breast cancer (TNBC) typically receive chemotherapy, surgery, and radiation therapy. Although this treatment improves prognosis for most patients, some patients continue to experience recurrence within 5 years. Preclinical studies have shown that immune cell infiltration at the irradiated site may play a significant role in tumor cell recruitment; however, little is known
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A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-10 Rachel R. Besser, Annie C. Bowles, Ahmad Alassaf, Daniel Carbonero, Renata Maciel, Mario Saporta, Ashutosh Agarwal
Introduction Multicellular platforms and linked multi organ on chip devices are powerful tools for drug discovery, and basic mechanistic studies. Often, a critical constraint is defining a culture medium optimal for all cells present in the system. In this study, we focused on the key cells of the neuromuscular junction i.e., skeletal muscle and motor neurons. Methods Formulation of a chemically defined
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Transdermal Delivery of Kidney-Targeting Nanoparticles Using Dissolvable Microneedles Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-06-01 Nirmalya Tripathy, Jonathan Wang, Madelynn Tung, Claire Conway, Eun Ji Chung
Introduction Chronic kidney disease (CKD) affects approximately 13% of the world’s population and will lead to dialysis or kidney transplantation. Unfortunately, clinically available drugs for CKD show limited efficacy and toxic extrarenal side effects. Hence, there is a need to develop targeted delivery systems with enhanced kidney specificity that can also be combined with a patient-compliant administration
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Early Intervention in Ischemic Tissue with Oxygen Nanocarriers Enables Successful Implementation of Restorative Cell Therapies Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-29 Ludmila Diaz-Starokozheva, Devleena Das, Xiangming Gu, Jordan T. Moore, Luke R. Lemmerman, Ian Valerio, Heather M. Powell, Natalia Higuita-Castro, Michael R. Go, Andre F. Palmer, Daniel Gallego-Perez
Background Tissue ischemia contributes to necrosis and infection. While angiogenic cell therapies have emerged as a promising strategy against ischemia, current approaches to cell therapies face multiple hurdles. Recent advances in nuclear reprogramming could potentially overcome some of these limitations. However, under severely ischemic conditions necrosis could outpace reprogramming-based repair
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Neonatal Fibrin Scaffolds Promote Enhanced Cell Adhesion, Migration, and Wound Healing In Vivo Compared to Adult Fibrin Scaffolds Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-27 Kimberly Nellenbach, Seema Nandi, Christopher Peeler, Alexander Kyu, Ashley C. Brown
Introduction Fibrin scaffolds are often utilized to treat chronic wounds. The monomer fibrinogen used to create such scaffolds is typically derived from adult human or porcine plasma. However, our previous studies have identified extensive differences in fibrin network properties between adults and neonates, including higher fiber alignment in neonatal networks. Wound healing outcomes have been linked
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Naturally Derived Membrane Lipids Impact Nanoparticle-Based Messenger RNA Delivery. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-26 Jeonghwan Kim,Antony Jozic,Gaurav Sahay
Introduction Lipid based nanoparticles (LNPs) are clinically successful vectors for hepatic delivery of nucleic acids. These systems are being developed for non-hepatic delivery of mRNA for the treatment of diseases like cystic fibrosis or retinal degeneration as well as infectious diseases. Localized delivery to the lungs requires aerosolization. We hypothesized that structural lipids within LNPs
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Endothelial Cells Morphology in Response to Combined WSS and Biaxial CS: Introduction of Effective Strain Ratio Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-12 Hossein Ali Pakravan, Mohammad Said Saidi, Bahar Firoozabadi
Introduction Endothelial cells (ECs) morphology strongly depends on the imposed mechanical stimuli. These mechanical stimuli include wall shear stress (WSS) and biaxial cyclic stretches (CS). Under combined loading, the effect of CS is not as simple as pure CS. The present study investigates the morphological response of ECs to the realistic mechanical stimuli. Methods The cell population is theoretically
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Cyclic Strain Promotes H19 Expression and Vascular Tube Formation in iPSC-Derived Endothelial Cells. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-07 Mark J Vander Roest,W David Merryman
IntroductionInduced pluripotent stem cell (iPSC)-derived endothelial cells (ECs) have the potential for therapeutic application in several cardiovascular diseases. Mechanical strain is known to regulate EC behavior and stem cell differentiation and may play a role in directing EC differentiation of iPSCs. H19, a long non-coding RNA (lncRNA), is known to affect ECs in several mechanically relevant pathologies
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A Multiscale Model to Predict Neuronal Cell Deformation with Varying Extracellular Matrix Stiffness and Topography. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-04 Mohan Yasodharababu,Arun K Nair
IntroductionNeuronal cells are sensitive to mechanical properties of extracellular matrix (ECM) such as stiffness and topography. Cells contract and exert a force on ECM to detect the microenvironment, which activates the signaling pathway to influence the cell functions such as differentiation, migration, and proliferation. There are numerous transmembrane proteins that transmit signals; however,
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Targeting Dysfunctional Vascular Endothelial Cells Using Immunoliposomes Under Flow Conditions. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-05-04 Mahsa Kheradmandi,Ian Ackers,Monica M Burdick,Ramiro Malgor,Amir M Farnoud
IntroductionAtherosclerosis (ATH), the build up of fat in the arteries, is a principal cause of heart attack and stroke. Drug instability and lack of target specificity are major drawbacks of current clinical therapeutics. These undesirable effects can be eliminated by site-specific drug delivery. The endothelial surface over ATH lesions has been shown to overexpress vascular cell adhesion molecule1
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Effect of Sintering Temperature of Bioactive Glass Nanoceramics on the Hemolytic Activity and Oxidative Stress Biomarkers in Erythrocytes. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-04-03 Ioannis Tsamesidis,Konstantina Kazeli,Evgenia Lymperaki,Georgia K Pouroutzidou,Ilias M Oikonomou,Philomela Komninou,George Zachariadis,Karine Reybier,Antonella Pantaleo,Eleana Kontonasaki
IntroductionThe nature of the surface is critical in determining the biological activity of silica powders. A novel correlation between toxicity and surface properties of bioactive glass ceramics (BGCs) synthesized via the sol–gel method was attempted in this study. MethodsThe behavior of BGCs after their attachment to the surface of red blood cells (RBCs) was evaluated and their toxic effects were
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Evaluation of the Antihemostatic and Antithrombotic Effects of Lowering Coagulation Factor VII Levels in a Non-human Primate. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-03-23 Michael Wallisch,Sven R Olson,Jeffrey Crosby,Jennifer Johnson,Susan F Murray,Joseph J Shatzel,Erik I Tucker,Owen J T McCarty,Monica T Hinds,Brett P Monia,András Gruber
IntroductionTissue factor (TF) and factor (F) VII, components of the extrinsic pathway of blood coagulation, are essential for hemostatic plug formation in response to injury; less clear are their roles in propagating thrombosis, as observational data in humans with congenital FVII deficiency suggests persistent thrombotic and bleeding risk even at significantly decreased FVII levels. We aimed to define
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Machine Learning Classification of Articular Cartilage Integrity Using Near Infrared Spectroscopy. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-03-09 Isaac O Afara,Jaakko K Sarin,Simo Ojanen,Mikko A J Finnilä,Walter Herzog,Simo Saarakkala,Rami K Korhonen,Juha Töyräs
IntroductionAssessment of cartilage integrity during arthroscopy is limited by the subjective visual nature of the technique. To address this shortcoming in diagnostic evaluation of articular cartilage, near infrared spectroscopy (NIRS) has been proposed. In this study, we evaluated the capacity of NIRS, combined with machine learning techniques, to classify cartilage integrity. MethodsRabbit (n = 14)
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Correction to: Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2019-09-17 Wafa Altalhi,Rupal Hatkar,James B Hoying,Yasaman Aghazadeh,Sara S Nunes
[This corrects the article DOI: 10.1007/s12195-019-00574-3.].
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Microvascular Mimetics for the Study of Leukocyte-Endothelial Interactions. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-01-31 Tejas S Khire,Alec T Salminen,Harsha Swamy,Kilean S Lucas,Molly C McCloskey,Raquel E Ajalik,Henry H Chung,Thomas R Gaborski,Richard E Waugh,Angela J Glading,James L McGrath
IntroductionThe pathophysiological increase in microvascular permeability plays a well-known role in the onset and progression of diseases like sepsis and atherosclerosis. However, how interactions between neutrophils and the endothelium alter vessel permeability is often debated. MethodsIn this study, we introduce a microfluidic, silicon-membrane enabled vascular mimetic (μSiM-MVM) for investigating
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Localization of Rolling and Firm-Adhesive Interactions Between Circulating Tumor Cells and the Microvasculature Wall. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-01-24 Mahsa Dabagh,John Gounley,Amanda Randles
IntroductionThe adhesion of tumor cells to vessel wall is a critical stage in cancer metastasis. Firm adhesion of cancer cells is usually followed by their extravasation through the endothelium. Despite previous studies identifying the influential parameters in the adhesive behavior of the cancer cell to a planer substrate, less is known about the interactions between the cancer cell and microvasculature
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Sticking to the Problem: Engineering Adhesion in Molecular Endoscopic Imaging. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2020-01-21 Mahboubeh S Noori,Sarah J Bodle,Christian A Showalter,Evan S Streator,David S Drozek,Monica M Burdick,Douglas J Goetz
Cancers of the digestive tract cause nearly one quarter of the cancer deaths worldwide, and nearly half of these are due to cancers of the esophagus and colon. Early detection of cancer significantly increases the rate of survival, and thus it is critical that cancer within these organs is detected early. In this regard, endoscopy is routinely used to screen for transforming/cancerous (i.e. dysplastic
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Transplantation of IL-10-Overexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorates Diabetic-Induced Impaired Fracture Healing in Mice. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2019-12-23 Keze Cui,Yuanliang Chen,Haibo Zhong,Nan Wang,Lihui Zhou,Fusong Jiang
BackgroundDiabetes mellitus is characterized by hyperglycemia which displays insufficiency or resistance to insulin. One of the complications of diabetes is the increased risk of fracture and the impairment of bone repair and regulation. There have been evidences from previous studies that mesenchymal stem cells (MSCs) from bone marrow promote cartilage and callous formation. In addition, IL-10, an
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Engineering of Exosomes to Target Cancer Metastasis. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2019-12-23 Zhenjiang Zhang,Jenna A Dombroski,Michael R King
As a nanoscale subset of extracellular vehicles, exosomes represent a new pathway of intercellular communication by delivering cargos such as proteins and nucleic acids to recipient cells. Importantly, it has been well documented that exosome-mediated delivery of such cargo is involved in many pathological processes such as tumor progression, cancer metastasis, and development of drug resistance. Innately
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A Stable Pep2-proapoptotic Peptide Inducing Apoptosis of Acute Myeloid Leukemia Cells by Down-Regulating EZH2. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2019-12-04 Yanli Sun,Jiaqiu Li,Yanhua Sun,Ronglan Zhao,Lujuan Wang,Wei Song,Zhanzhao Wang,Jialing Wang,Liuya Wei,Yao Zhao,Yang Song,Zhenbo Hu
IntroductionProapoptotic peptide, (KLAKLAK)2, exhibits strong anti-tumor effect with the help of cell-penetrating peptides such as Pep2, targeting TLR2 with high expression in acute myeloid leukemia (AML). However, the applications are limited due to the peptide’s instability and high cost of synthesis. Recombinant PP7 bacteriophage-like particles (VLPs) can protect the peptides from degradation by
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Synergistic Adhesiveness of Fibronectin with PHSRN Peptide in Gelatin Mixture Promotes the Therapeutic Potential of Human ES-Derived MSCs. Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2019-12-02 Hye-Seon Kim,Sung Hyun Choi,Mi-Lan Kang,Ki-Won Lee,Ki Nam Kim,Hak-Joon Sung