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Evaluation of prokaryotic and eukaryotic microbial communities on microplastic‐associated biofilms in marine and freshwater environments Eng. Life Sci. (IF 2.7) Pub Date : 2024-03-09 Şuheda Reisoglu, Ceren Cati, Meral Yurtsever, Sevcan Aydin
Microplastics (MPs) are major concern due to their potential harm to ecosystems and most research has focused on their presence and fate, with limited attention to their biodegradation in aquatic ecosystems. Nevertheless, MPs act as hotspots for the colonization by a diverse range of microorganisms that can adhere to plastic surfaces, resulting in the subsequent formation of biofilms—a potential threat
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Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization Eng. Life Sci. (IF 2.7) Pub Date : 2024-03-08 Thomas Hahn, Melissa Ortega Alzate, Steven Leonhardt, Pravesh Tamang, Susanne Zibek
Polyhydroxyalkanoates (PHAs) have gained interest recently due to their biodegradability and versatility. In particular, the chemical compositions of medium‐chain‐length (mcl)‐PHAs are highly diverse, comprising different monomers containing 6–14 carbon atoms. This review summarizes different feedstocks and fermentation strategies to enhance mcl‐PHA production and briefly discusses the downstream processing
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Rapid exometabolome footprinting combined with multivariate statistics: A powerful tool for bioprocess optimization Eng. Life Sci. (IF 2.7) Pub Date : 2024-03-06 Alexander Reiter, Lars Wesseling, Wolfgang Wiechert, Marco Oldiges
Corynebacterium glutamicum is used as an industrial platform organism for amino acid production. Previously, the organism was utilized to produce l‐histidine with research focusing on metabolic engineering approaches to increase titer and yield. Only a few studies have been published that provide information on bioprocess development, with media optimization and fed‐batch cultivation procedure being
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From single‐cell cloning to high‐yield influenza virus production – implementing advanced technologies in vaccine process development Eng. Life Sci. (IF 2.7) Pub Date : 2024-02-19 Tilia Zinnecker, Najd Badri, Diogo Araujo, Kristin Thiele, Udo Reichl, Yvonne Genzel
Innovations in viral vaccine manufacturing are crucial for pandemic preparedness and to meet ever‐rising global demands. For influenza, however, production still mainly relies on technologies established decades ago. Although modern production shifts from egg‐based towards cell culture technologies, the full potential has not yet been fully exploited. Here, we evaluate whether implementation of state‐of‐the‐art
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Digitalization concepts in academic bioprocess development Eng. Life Sci. (IF 2.7) Pub Date : 2024-02-09 Tessa Habich, Sascha Beutel
Digitalization with integrated devices, digital and physical assistants, automation, and simulation is setting a new direction for laboratory work. Even with complex research workflows, high staff turnover, and a limited budget some laboratories have already shown that digitalization is indeed possible. However, academic bioprocess laboratories often struggle to follow the trend of digitalization.
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Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building Eng. Life Sci. (IF 2.7) Pub Date : 2023-12-11 Hannah Marienberg, Nicole Desch, Vitalii Mozin, Lorenz Sykora-Mirle, Anja Müller, Andreas Roth, Mathias Käfer, Rüdiger Neef
Process Analytical Technologies (PATs) are taking a key role in the run for automatization in the biopharmaceutical industry. Spectroscopic methods such as Raman spectroscopy or mid-infrared (MIR) spectroscopy are getting more recognition in the recent years for inline monitoring of bioprocesses due to their ability to measure various molecules simultaneously. However, their dependency on laborious
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Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale Eng. Life Sci. (IF 2.7) Pub Date : 2023-12-08 Vedran Vučić, Hauke Harms, Susann Müller
Most recent advances for phosphorus (P) recovery using brewery yeast on laboratory scale were used to scale up to a pilot-scale process (BioP-Rec module) and applied in a full-scale wastewater treatment plant (WWTP). A P balance was established for WWTP Markranstädt according to two thresholds: (1) the economic feasibility threshold for P recovery of 0.05 kg/m3 of free P, and (2) the German Sewage
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Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification Eng. Life Sci. (IF 2.7) Pub Date : 2023-12-06 Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, Alper Şişman
We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans
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Cultivated meat manufacturing: Technology, trends, and challenges Eng. Life Sci. (IF 2.7) Pub Date : 2023-11-20 Marline Kirsch, Jordi Morales-Dalmau, Antonina Lavrentieva
The growing world population, public awareness of animal welfare, environmental impacts and changes in meat consumption leads to the search for novel approaches to food production. Novel foods include products with a new or specifically modified molecular structure, foods made from microorganisms, fungi, algae or insects, as well as from animal cell or tissue cultures. The latter approach is known
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Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives Eng. Life Sci. (IF 2.7) Pub Date : 2023-11-13 Xinyu Zhu, Ping Li, Feng Ju
Biowaste fermentation is a promising technology for low-carbon print bioenergy and biochemical production. Although it is believed that the microbiome determines both the fermentation efficiency and the product profiles of biowastes, the explicit mechanisms of how microbial activity controls fermentation processes remained to be unexplored. The current study investigated the microbiome dynamics and
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Flavonoids, gut microbiota, and host lipid metabolism Eng. Life Sci. (IF 2.7) Pub Date : 2023-11-13 Miao Zhou, Jie Ma, Meng Kang, Wenjie Tang, Siting Xia, Jie Yin, Yulong Yin
Flavonoids are widely distributed in nature and have a variety of beneficial biological effects, including antioxidant, anti-inflammatory, and anti-obesity effects. All of these are related to gut microbiota, and flavonoids also serve as a bridge between the host and gut microbiota. Flavonoids are commonly used to modify the composition of the gut microbiota by promoting or inhibiting specific microbial
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Endospore production of Bacillus spp. for industrial use Eng. Life Sci. (IF 2.7) Pub Date : 2023-10-12 Riekje Biermann, Sascha Beutel
The increased occurrence of antibiotic resistance and the harmful use of pesticides are a major problem of modern times. A ban on the use of antibiotics as growth promoters in animal breeding has put a focus on the probiotics market. Probiotic food supplements are versatile and show promising results in animal and human nutrition. Chemical pesticides can be substituted by biopesticides, which are very
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Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions Eng. Life Sci. (IF 2.7) Pub Date : 2023-10-05 Sevda Zoghi, Fatemah Sadeghpour Heravi, Zeinab Nikniaz, Masoud Shirmohamadi, Seyed Yaghoub Moaddab, Hamed Ebrahimzadeh Leylabadlo
Childhood malnutrition is a metabolic condition that affects the physical and mental well-being of children and leads to resultant disorders in maturity. The development of childhood malnutrition is influenced by a number of physiological and environmental factors including metabolic stress, infections, diet, genetic variables, and gut microbiota. The imbalanced gut microbiota is one of the main environmental
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Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium Eng. Life Sci. (IF 2.7) Pub Date : 2023-09-15 Riekje Biermann, Laura Rösner, Lisa-Marie Beyer, Laura Niemeyer, Sascha Beutel
Bacillus coagulans is a promising probiotic, because it combines probiotic properties of Lactobacillus and the ability of Bacillus to form endospores. Due to this hybrid relationship, cultivation of this organism is challenging. As the probiotics market continues to grow, there is a new focus on the production of these microorganisms. In this work, a strain-specific bioprocess for B. coagulans was
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Integration of a perfusion reactor and continuous precipitation in an entirely membrane-based process for antibody capture Eng. Life Sci. (IF 2.7) Pub Date : 2023-09-07 Gabriele Recanati, Magdalena Pappenreiter, Christoph Gstoettner, Patrick Scheidl, Elena Domínguez Vega, Bernhard Sissolak, Alois Jungbauer
Continuous precipitation coupled with continuous tangential flow filtration is a cost-effective alternative for the capture of recombinant antibodies from crude cell culture supernatant. The removal of surge tanks between unit operations, by the adoption of tubular reactors, maintains a continuous harvest and mass flow of product with the advantage of a narrow residence time distribution (RTD). We
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Investigation and evaluation of a 3D-printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms Eng. Life Sci. (IF 2.7) Pub Date : 2023-08-23 Johanna S. Rehfeld, Louis M. Kuhnke, Christian Ude, Gernot T. John, Sascha Beutel
In the field of bioprocess development miniaturization, parallelization and flexibility play a key role reducing costs and time. To precisely meet these requirements, additive manufacturing (3D-printing) is an ideal technology. 3D-printing enables rapid prototyping and cost-effective fabrication of individually designed devices with complex geometries on demand. For successful bioprocess development
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Meta-omics assisted microbial gene and strain resources mining in contaminant environment Eng. Life Sci. (IF 2.7) Pub Date : 2023-08-18 Yiqun Huang, Haiyang Hu, Tingting Zhang, Weiwei Wang, Wenzhao Liu, Hongzhi Tang
Human activities have led to the release of various environmental pollutants, triggering ecological challenges. In situ, microbial communities in these contaminated environments are usually assumed to possess the potential capacity of pollutant degradation. However, the majority of genes and microorganisms in these environments remain uncharacterized and uncultured. The advent of meta-omics provided
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Evaluation of restricted access media for the purification of cell culture-derived Orf viruses Eng. Life Sci. (IF 2.7) Pub Date : 2023-07-14 Keven Lothert, Yasmina M. J. Harsy, Patrick Endres, Egbert Müller, Michael W. Wolff
Recently, multimodal chromatography using restricted access media (RAM) for the purification of nanoparticles, such as viruses has regained increasing attention. These chromatography resins combine size exclusion on the particle shell and adsorptive interaction within the core. Accordingly, smaller process-related impurities, for example, DNA and proteins, can be retained, while larger product viruses
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Identification and characterization of inulinases by bioinformatics analysis of bacterial glycoside hydrolases family 32 (GH32) Eng. Life Sci. (IF 2.7) Pub Date : 2023-07-09 Fatemeh Khosravi, Ehsan Mohseni Fard, Marzieh Hosseininezhad, Hadi Shoorideh
The glycoside hydrolase family contains enzymes that break the glycosidic bonds of carbohydrates by hydrolysis. Inulinase is one of the most important industrial enzymes in the family of Glycoside Hydrolases 32 (GH32). In this study, to identify and classify bacterial inulinases initially, 16,002 protein sequences belonging to the GH32 family were obtained using various databases. The inulin-effective
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Multi-objective Bayesian algorithm automatically discovers low-cost high-growth serum-free media for cellular agriculture application Eng. Life Sci. (IF 2.7) Pub Date : 2023-06-28 Zachary Cosenza, David E. Block, Keith Baar, Xingyu Chen
In this work, we applied a multi-information source modeling technique to solve a multi-objective Bayesian optimization problem involving the simultaneous minimization of cost and maximization of growth for serum-free C2C12 cells using a hyper-volume improvement acquisition function. In sequential batches of custom media experiments designed using our Bayesian criteria, collected using multiple assays
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Evaluating scaling of capillary photo-biofilm reactors for high cell density cultivation of mixed trophies artificial microbial consortia Eng. Life Sci. (IF 2.7) Pub Date : 2023-06-23 Amelie Kenkel, Rohan Karande, Katja Bühler
Capillary biofilm reactors (CBRs) are attractive for growing photoautotrophic bacteria as they allow high cell-density cultivation. Here, we evaluated the CBR system's suitability to grow an artificial consortium composed of Synechocystis sp. PCC 6803 and Pseudomonas sp. VBL120. The impact of reactor material, flow rate, pH, O2, and medium composition on biomass development and long-term biofilm stability
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Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering Eng. Life Sci. (IF 2.7) Pub Date : 2023-06-13 Fatemeh Sabet Sarvestani, Ali-Mohammad Tamaddon, Ramin Yaghoobi, Bita Geramizadeh, Negar Azarpira
Angiogenesis is a vital step in tissue regeneration. Hence, the current study aimed to prepare oxidized dextran (Odex)/collagen (Col)-hydrogels with laminin (LMN), as an angiogenic extracellular matrix (ECM) component, for promoting human umbilical vein endothelial cell (HUVEC) proliferation and function. Odex/Col scaffolds were constructed at various concentrations and temperatures. Using oscillatory
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Gut microbiota induced epigenetic modifications in the non-alcoholic fatty liver disease pathogenesis Eng. Life Sci. (IF 2.7) Pub Date : 2023-06-02 Ruiqi Tang, Rongrong Liu, Hua Zha, Yiwen Cheng, Zongxin Ling, Lanjuan Li
Non-alcoholic fatty liver disease (NAFLD) represents a growing global health concern that can lead to liver disease and cancer. It is characterized by an excessive accumulation of fat in the liver, unrelated to excessive alcohol consumption. Studies indicate that the gut microbiota-host crosstalk may play a causal role in NAFLD pathogenesis, with epigenetic modification serving as a key mechanism for
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Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression Eng. Life Sci. (IF 2.7) Pub Date : 2023-05-24 Patrick Diep, Heping Leo Shen, Julian A. Wiesner, Nadia Mykytczuk, Vladimiros Papangelakis, Alexander F. Yakunin, Radhakrishnan Mahadevan
Mine wastewater often contains dissolved metals at concentrations too low to be economically extracted by existing technologies, yet too high for environmental discharge. The most common treatment is chemical precipitation of the dissolved metals using limestone and subsequent disposal of the sludge in tailing impoundments. While it is a cost-effective solution to meet regulatory standards, it represents
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Salting-out extraction of recombinant κ-carrageenase and phage T7 released from Escherichia coli cells Eng. Life Sci. (IF 2.7) Pub Date : 2023-05-17 Da Chen, Yue-Sheng Dong, Yong-Ming Bao, Zhi-Long Xiu
Traditional technology of cell disruption has become one of the bottlenecks restricting the industrialization of genetic engineering products due to its high cost and low efficiency. In this study, a novel bioprocess of phage lysis coupled with salting-out extraction (SOE) was evaluated. The lysis effect of T7 phage on genetically engineered Escherichia coli expressing κ-carrageenase was investigated
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Online deployment of an O-PLS model for dielectric spectroscopy-based inline monitoring of viable cell concentrations in Chinese hamster ovary cell perfusion cultivations Eng. Life Sci. (IF 2.7) Pub Date : 2023-05-17 Johannes Lemke, Robert Söldner, Jonas Austerjost
Viable cell concentration (VCC) is an essential parameter that is required to support the efficient cultivation of mammalian cells. Although commonly determined using at-line or off-line analytics, in-line capacitance measurements represent a suitable alternative method for the determination of VCC. In addition, these latter efforts are complimentary with the Food and Drug Administration's initiative
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Evaluating microbial contaminations of alternative heating oils Eng. Life Sci. (IF 2.7) Pub Date : 2023-05-05 Maximilian J. Surger, Katharina Mayer, Karthik Shivaram, Felix Stibany, Wilfried Plum, Andreas Schäffer, Simon Eiden, Lars M. Blank
Since 2008, European and German legislative initiatives for climate protection and reduced dependency on fossil resources led to the introduction of biofuels as CO2-reduced alternatives in the heating oil sector. In the case of biodiesel, customers were confronted with accelerated microbial contaminations during storage. Since then, other fuel alternatives, like hydrogenated vegetable oils (HVOs),
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Design of cell expansion processes for adherent-growing cells with mDoE-workflow Eng. Life Sci. (IF 2.7) Pub Date : 2023-04-25 Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller
Adherent cells, mammalian or human, are ubiquitous for production of viral vaccines, in gene therapy and in immuno-oncology. The development of a cell-expansion process with adherent cells is challenging as scale-up requires the expansion of the cell culture surface. Microcarrier (MC)-based cultures are still predominate. However, the development of MC processes from scratch possesses particular challenges
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Application of Artificial Neural Network for predicting biomass growth during domestic wastewater treatment through a biological process Eng. Life Sci. (IF 2.7) Pub Date : 2023-04-13 Mpho Muloiwa, Megersa Dinka, Stephen Nyende-Byakika
The biological treatment process is responsible for removing organic and inorganic matter in wastewater. This process relies heavily on microorganisms to successfully remove organic and inorganic matter. The aim of the study was to model biomass growth in the biological treatment process. Multilayer perceptron (MLP) Artificial Neural Network (ANN) algorithm was used to model biomass growth. Three metrics:
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Site-directed mutagenesis improves the practical application of L-glutamic acid decarboxylase in Escherichia coli Eng. Life Sci. (IF 2.7) Pub Date : 2023-03-09 Liu Fengmin, Zhang Heng, Zhang Xiangjun, Wei Xiaobo, Liu Huiyan, Fang Haitian
γ-Aminobutyric acid (GABA) is a kind of non-proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries. Enzymatic conversion is an efficient method to produce GABA, whereby glutamic acid decarboxylase (GAD) is the key enzyme that catalyzes the process. The activity of wild-type GAD is usually limited by temperature, pH or biotin concentration
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Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9-mediated knock-in in mammalian cells Eng. Life Sci. (IF 2.7) Pub Date : 2023-03-09 Seul Mi Kim, Jaejin Lee, Jae Seong Lee
CRISPR/Cas9-mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock-in (KI) efficiency is critical for fully implementing TI-mediated cell line development (CLD); however, such identification is cumbersome. In this study, we developed
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Membrane inlet—ion mobility spectrometry with automatic spectra evaluation as online monitoring tool for the process control of microalgae cultivation Eng. Life Sci. (IF 2.7) Pub Date : 2023-02-28 Malcolm Cämmerer, Thomas Mayer, Carolin Schott, Juliane Steingroewer, Ralf Petrich, Helko Borsdorf
The cultivation of algae either in open raceway ponds or in closed bioreactors could allow the renewable production of biomass for food, pharmaceutical, cosmetic, or chemical industries. Optimal cultivation conditions are however required to ensure that the production of these compounds is both efficient and economical. Therefore, high-frequency analytical measurements are required to allow timely
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Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed-batch (CFB) mode Eng. Life Sci. (IF 2.7) Pub Date : 2023-02-07 Yiling Lu, Jun Lin, Tianze Bian, Jin Chen, Dan Liu, Mingjun Ma, Zhen Gao, Jiemin Chen, Dianwen Ju, Xing Wang
Multiple control strategies, including a downstream purification process with well-controlled parameters and a comprehensive release or characterization for intermediates or drug substances, were implemented to mitigate the potential risk of host cell proteins (HCPs) in one concentrated fed-batch (CFB) mode manufactured product. A host cell process specific enzyme-linked immunosorbent assay (ELISA)
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An optimized live bacterial delivery vehicle safely and efficaciously delivers bacterially transcribed therapeutic nucleic acids Eng. Life Sci. (IF 2.7) Pub Date : 2023-02-05 Darcy S. O. Mora, Madeline Cox, Forgivemore Magunda, Ashley B. Williams, Lyndsey Linke
There is an unmet need for delivery platforms that realize the full potential of next-generation nucleic acid therapeutics. The in vivo usefulness of current delivery systems is limited by numerous weaknesses, including poor targeting specificity, inefficient access to target cell cytoplasm, immune activation, off-target effects, small therapeutic windows, limited genetic encoding and cargo capacity
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Rapid in-situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome Eng. Life Sci. (IF 2.7) Pub Date : 2023-02-02 Song Xu, Lidan Tao, Jingjing Wang, Xiaoxia Zhang, Zhiyong Huang
The high salt content of food waste (FW) severely limits microbial physiological activity and reduces its biodegradability. In this study, a salt-tolerant thermophilic bacterial agent that consists of four different substrate degradation functional strains was evaluated for efficient high salt and oily FW in solid-state aerobic biodegradation disposers. The phy-chemical properties, enzyme activities
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Escherichia coli aceE variants coding pyruvate dehydrogenase improve the generation of pyruvate-derived acetoin Eng. Life Sci. (IF 2.7) Pub Date : 2023-01-31 W. Chris Moxley, Rachel E. Brown, Mark A. Eiteman
Several chromosomally expressed AceE variants were constructed in Escherichia coli ΔldhA ΔpoxB ΔppsA and compared using glucose as the sole carbon source. These variants were examined in shake flask cultures for growth rate, pyruvate accumulation, and acetoin production via heterologous expression of the budA and budB genes from Enterobacter cloacae ssp. dissolvens. The best acetoin-producing strains
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Cell-free synthesis of silver nanoparticles in spent media of different Aspergillus species Eng. Life Sci. (IF 2.7) Pub Date : 2023-01-12 Alexander Boldt, Jan Walter, Fabian Hofbauer, Karen Stetter, Ines Aubel, Martin Bertau, Christof M. Jäger, Thomas Walther
The recovery and valorization of metals and rare earth metals from wastewater are of great importance to prevent environmental pollution and recover valuable resources. Certain bacterial and fungal species are capable of removing metal ions from the environment by facilitating their reduction and precipitation. Even though the phenomenon is well documented, little is known about the mechanism. Therefore
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Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement Eng. Life Sci. (IF 2.7) Pub Date : 2023-01-10 You Wang, Yushu Wang, Yuqi Wu, Yang Suo, Huaqing Guo, Yineng Yu, Ruonan Yin, Rui Xi, Jiajie Wu, Nan Hua, Yuehan Zhang, Shaobo Zhang, Zhenming Jin, Lin He, Gang Ma
Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid
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Editorial for the special issue ‘cell-to-cell’ and ‘cell-to-bioreactor’ interactions Eng. Life Sci. (IF 2.7) Pub Date : 2023-01-04 Ralf Takors
Shifting the current fossil economy to a carbon neutral supply is an enormous challenge. Current geopolitical issues that severely endanger long-term established fossil supply chains function as catalysts fostering novel solutions. In this context, the goals for establishing a resilient economy meet climate protection demands for reducing the human footprint in atmosphere. Biotechnological processes
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Mobile SARS‑CoV‑2 screening facilities for rapid deployment and university-based diagnostic laboratory Eng. Life Sci. (IF 2.7) Pub Date : 2023-01-03 Nils Stanislawski, Ferdinand Lange, Christian Fahnemann, Christoph Riggers, Marc-Nils Wahalla, Marc Porr, Fabian Cholewa, Rebecca Jonczyk, Stefanie Thoms, Martin Witt, Frank Stahl, Sascha Beutel, Andreas Winkel, Philipp-Cornelius Pott, Meike Stiesch, Mira Paulsen, Anette Melk, Henning Lucas, Stefanie Heiden, Holger Blume, Cornelia Blume
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created a public crisis. Many medical and public institutions and businesses went into isolation in response to the pandemic. Because SARS-CoV-2 can spread irrespective of a patient's course of disease, these institutions’ continued operation or reopening based on the assessment and control of virus spread can be supported
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In-line monitoring of protein concentration with MIR spectroscopy during UFDF Eng. Life Sci. (IF 2.7) Pub Date : 2022-12-23 Adrianna Milewska, Géraldine Baekelandt, Sarra Boutaieb, Vitalii Mozin, Andrew Falconbridge
Rapid increase of product titers in upstream processes has presented challenges for downstream processing, where purification costs increase linearly with the increase of the product yield. Hence, innovative solutions are becoming increasingly popular. Process Analytical Technology (PAT) tools, such as spectroscopic techniques, are on the rise due to their capacity to provide real-time, precise analytics
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Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies Eng. Life Sci. (IF 2.7) Pub Date : 2022-12-23 Ole Jacob Wohlenberg, Carlotta Kortmann, Katharina V. Meyer, Thomas Scheper, Dörte Solle
Quality by Design (QbD) is one of the most important tools for the implementation of Process Analytical Technology (PAT) in biopharmaceutical production. For optimal characterization of a monoclonal antibody (mAb) upstream process a stepwise approach was implemented. The upstream was divided into three process stages, namely inoculum expansion, production, and primary recovery, which were investigated
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Regulation on C2-C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation Eng. Life Sci. (IF 2.7) Pub Date : 2022-12-13 Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang
Bioconversion of CO2 into liquid fuels or chemicals, preferred medium chain carboxylic acids (caproic and caprylic acid), is an attractive CO2 utilization technology. The present study aims to investigate the effects of different ratios of H2/CO2 on regulating the distribution of C2-C8 carboxylic acid products, while the headspace pressure of 1.5 bar was set to amplify the effect of different ratios
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Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production Eng. Life Sci. (IF 2.7) Pub Date : 2022-12-07 Yaxue He, Chiara Cassarini, Piet N.L. Lens
An anaerobic granular sludge was enriched to utilize H2/CO2 in a continuous gas-fed up-flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H2/CO2 was
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Retraction Statement: Comparative molecular docking and molecular-dynamic simulation of wild-type- and mutant carboxylesterase with BTA-hydrolase for enhanced binding to plastic Eng. Life Sci. (IF 2.7) Pub Date : 2022-11-23
Retraction: ‘Comparative molecular docking and molecular-dynamic simulation of wild-type- and mutant carboxylesterase with BTA-hydrolase for enhanced binding to plastic’, by Fatana Lameh, Abdul Qadeer Baseer, and Abubakar Garba Ashiru, Eng Life Sci. 2021; 13-29: The above article, published online on 15 November 2021 in Wiley Online Library (https://doi.org/10.1002/elsc.202100083), has been retracted
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Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large-eddy simulation Eng. Life Sci. (IF 2.7) Pub Date : 2022-11-11 Navraj Hanspal, Brian DeVincentis, John A. Thomas
We present a transient large eddy simulation (LES) modeling approach for simulating the interlinked physics describing free surface hydrodynamics, multiphase mixing, reaction kinetics, and mass transport in bioreactor systems. Presented case-studies include non-reacting and reacting bioreactor systems, modeled through the inclusion of uniform reaction rates and more complex biochemical reactions described
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Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures Eng. Life Sci. (IF 2.7) Pub Date : 2022-09-13 Sofia Ribeiro, Eugenia Pugliese, Stefanie H. Korntner, Emanuel M. Fernandes, Manuela E. Gomes, Rui L. Reis, Alan O'Riordan, Yves Bayon, Dimitrios I. Zeugolis
The combined effect of surface topography and substrate rigidity in stem cell cultures is still under-investigated, especially when biodegradable polymers are used. Herein, we assessed human bone marrow stem cell response on aliphatic polyester substrates as a function of anisotropic grooved topography and rigidity (7 and 12 kPa). Planar tissue culture plastic (TCP, 3 GPa) and aliphatic polyester substrates
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Preparation of a pH-responsive chitosan-montmorillonite-nitrogen-doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy Eng. Life Sci. (IF 2.7) Pub Date : 2022-09-13 Erfan Rahmani, Mehrab Pourmadadi, Sohrab Ali Ghorbanian, Fatemeh Yazdian, Hamid Rashedi, Mona Navaee
Despite its widespread usage as a chemotherapy drug in cancer treatment, doxorubicin (DOX) has limitations such as short in vivo circulation time, low solubility, and poor permeability. In this regard, a pH-responsive chitosan (CS)- montmorillonite (MMT)- nitrogen-doped carbon quantum dots (NCQDs) nanocomposite was first developed, loaded with DOX, and then incorporated into a double emulsion to further
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Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-30 Omprakash Sarkar, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas
The present study reports the mixed culture acidogenic production of biohydrogen and carboxylic acids (CA) from brewery spent grains (BSG) in the presence of high concentrations of cobalt, iron, nickel, and zinc. The metals enhanced biohydrogen output by 2.39 times along with CA biosynthesis by 1.73 times. Cobalt and iron promoted the acetate and butyrate pathways, leading to the accumulation of 5
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Evaluation of SDS-coated iron nanostructure on the gene expression of bio surfactant-producing genes by Pseudomonas aeruginosa Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-24 Yaser Ahsani Arani, Zahra Noormohammadi, Behnam Rasekh, Fatemeh Yazdian, Hojjat kazemi
Bio surfactants are natural surfactants that induce emulsification, displacement, increased solubility, and mobility of hydrophobic organic compounds. In this study, the gene expression of biosurfactant production genes by Pseudomonas aeruginosa in the presence of sodium dodecyl sulfate coated iron nanostructure (Fe/SDS) were evaluated. Emulsification Index and Surface Tension reduction test to check
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Novel application of anti-human Fc nanobody for screening high-producing CHO cells for monoclonal antibody Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-23 Di Zhu, Zheng Wang, Yunxia Xu, Jing Lin, Mei Qiu, Jianghai Liu, Xinlei Li
Animal-derived anti-IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)-producing cells, but using animal-derived antibodies may raise the concerns of high cost, complicated operations and biological safety issues in biopharmaceutical manufacturing. Nanobodies(VHHs) are attractive forms of antibodies for their straightforward engineering and expression
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3D-printed autoclavable plant holders to facilitate large-scale protein production in plants Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-15 Ling Chuang, Anton Enders, Sascha Offermann, Janina Bahnemann, Jakob Franke
The Australian tobacco plant Nicotiana benthamiana is becoming increasingly popular as a platform for protein production and metabolic engineering. In this system, gene expression is achieved transiently by infiltrating N. benthamiana plants with suspensions of Agrobacterium tumefaciens carrying vectors with the target genes. To infiltrate larger numbers of plants, vacuum infiltration is the most efficient
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Removal of heavy metals from industrial wastewater using microbial fuel cell Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-03 Sameer Al-Asheh, Marzieh Bagheri, Ahmad Aidan
Removal efficiency of gold from a solution of pure tetrachloroaurate ions was investigated using microbial fuel cell (MFC) technology. The effects of type of catholyte solution and initial gold concentration on the removal efficiency were considered. Due to its presence at high levels in the gold wastewater, the effect of copper ions on the removal efficiency of the gold ions was also studied. The
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Corrigendum Eng. Life Sci. (IF 2.7) Pub Date : 2022-08-03
Large-scale production, purification, and function of a tumor multi-epitope vaccine: Peptibody with bFGF/VEGFA Ligang Zhang, Chengcheng Jiang, Xi Chen, Jiangtao Gu, Qifang Song, Hui Zhong, Sheng Xiong, Qingfeng Dong, Jin-Chen Yu, Ning Deng Eng. Life Sci. 2020, 20, 422–436 https://doi.org/10.1002/elsc.202000020 First published: 29 July 2020 For the above-mentioned article, concerns about image integrity
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Real-time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process Eng. Life Sci. (IF 2.7) Pub Date : 2022-07-27 Xiaoping Wang, Ying Tian, Sheng Zhang, Haibin Qu
Process analysis and monitoring during the manufacturing of the dripping pills are essential. However, research on developing sensor-based technology or process analytical technology (PAT) tools to analyze and monitor the dripping process is minimal. The purpose of this work is to develop a fast and non-destructive laser detection system for quantitative visualization of droplets, which involves detecting
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A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation Eng. Life Sci. (IF 2.7) Pub Date : 2022-07-18 Manuela Cassotta, Joanna Julia Bartnicka, Francesca Pistollato, Surat Parvatam, Tilo Weber, Vito D'Alessandro, Luisa Ferreira Bastos, Sandra Coecke
The use of cell and tissue-based methods in basic, applied and regulatory science has been increasing exponentially. Animal-derived components, including serum, coating materials, growth factors and antibodies are routinely used in cell/tissue cultures and in general laboratory practices. In addition to ethical issues, the use and production of animal-derived materials and reagents raises many scientific
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Flexible biofilm monitoring device Eng. Life Sci. (IF 2.7) Pub Date : 2022-07-12 Viktoria Maria Reiferth, Dirk Holtmann, Daniela Müller
Biofilms and their analysis are increasingly attracting the attention of the scientific community due to the immense importance and impact of biofilms in various natural, technical and medical fields. For these purposes, an optimized and extended antibiofilm assay system based on the Calgary Biofilm Device (MBEC Assay® system) consisting of microtiter plate and PCR tubes was established. Its implementation
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A UV-C LED-based unit for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter Eng. Life Sci. (IF 2.7) Pub Date : 2022-07-08 Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad v. Volkmann, Toralf Kaiser
Aseptic cell sorting is challenging, especially when a flow-cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi. Thus, a regular chemical cleaning procedure is required to prepare a sorter for aseptic cell sorting by flushing the fluidic system. However, this procedure is