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Regulatory effects of transition metals supplementation/deficiency on the gut microbiota Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Cheng-Yu Li, Xin-Yu Li, Liang Shen, Hong-Fang Ji
Abstract Transition metal ions are essential micronutrients for all living organisms and exert a wide range of effects on human health. The uptake of transition metal ions occurs primarily in the gastrointestinal tract, which is colonized by trillions of bacterial cells. In recent years, increasing studies have indicated that transition metals have regulatory effects on the gut microbiota. In view
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Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Ewelina Celińska, Jean-Marc Nicaud, Wojciech Białas
Abstract Consolidated bioprocessing (CBP) featuring concomitant hydrolysis of renewable substrates and microbial conversion into value-added biomolecules is considered to bring substantial benefits to the overall process efficiency. The biggest challenge in developing an economically feasible CBP process is identification of bifunctional biocatalyst merging the ability to utilize the substrate and
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Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Wojciech Makowski, Aleksandra Królicka, Anna Nowicka, Jana Zwyrtková, Barbara Tokarz, Ales Pecinka, Rafał Banasiuk, Krzysztof Michał Tokarz
Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in
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Enhanced human lysozyme production by Pichia pastoris via periodic glycerol and dissolved oxygen concentrations control Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Luqiang Jia, Teng Li, Yixuan Wu, Chunsen Wu, Huaxiang Li, Agen Huang
Abstract In human lysozyme (hLYZ) production by Pichia pastoris, the glycerol fed-batch phase was generally implemented under the environment of “oxygen sufficient-glycerol limited” to achieve high cell-density cultivation during the cell growth phase. However, the structural and functional components in P. pastoris cells were irreversible damaged with more and more reactive oxygen species (ROS) accumulation
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Probiotics ameliorate chronic low-grade inflammation and fat accumulation with gut microbiota composition change in diet-induced obese mice models Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Hyunchae Joung, Jaeryang Chu, Byoung-Kook Kim, In-Suk Choi, Woosang Kim, Tae-Sik Park
Recent reports suggest that obesity is caused by dysbiosis of gut microbiota and that it could be prevented or treated through improvement in the composition and diversity of gut microbiota. In this study, high-fat diet (HFD)-induced obese mice were orally administered with Lactobacillus plantarum K50 (K50) isolated from kimchi and Lactobacillus rhamnosus GG (LGG) as a positive control for 12 weeks
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Microbial consortia capable of reducing selenate in the presence of nitrate enriched from coalmining-impacted environments Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Frank Nkansah-Boadu, Ido Hatam, Susan A. Baldwin
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Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3) Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Christopher M. Jones, Travis C. Korosh, David R. Nielsen, Brian F. Pfleger
Abstract With the goal of expanding the diversity of tools available for controlling gene expression in cyanobacteria, the T7-RNA polymerase gene expression system from E. coli BL21(DE3) was adapted and systematically engineered for robust function Synechococcus sp. PCC 7002, a fast-growing saltwater strain. Expression of T7-RNA polymerase was controlled via LacI regulation, while functionality was
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MrPEX33 is involved in infection-related morphogenesis and pathogenicity of Metarhizium robertsii Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Zhangxun Wang, Jianyu Feng, Yuanyuan Jiang, Xiuzhen Xu, Liuyi Xu, Quan Zhou, Bo Huang
Abstract Peroxisomes, being indispensable organelles, play an important role in different biological processes in eukaryotes. PEX33, a filamentous fungus-specific peroxin of the docking machinery of peroxisomes, is involved in the virulence and development of other fungal pathogens. However, it is not clear whether PEX33 is necessary for the pathogenicity and development of an insect pathogenic fungus
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Simultaneous elucidation of antibiotic mechanism of action and potency with high-throughput Fourier-transform infrared (FTIR) spectroscopy and machine learning Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Bernardo Ribeiro da Cunha, Luís P. Fonseca, Cecília R. C. Calado
Abstract The low rate of discovery and rapid spread of resistant pathogens have made antibiotic discovery a worldwide priority. In cell-based screening, the mechanism of action (MOA) is identified after antimicrobial activity. This increases rediscovery, impairs low potency candidate detection, and does not guide lead optimization. In this study, high-throughput Fourier-transform infrared (FTIR) spectroscopy
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Effect of temperature on the production of a recombinant antivenom in fed-batch mode Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-14 Susana María Alonso Villela, Hazar Ghezal-Kraïem, Balkiss Bouhaouala-Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
In the pharmaceutical industry, nanobodies show promising properties for its application in serotherapy targeting the highly diffusible scorpion toxins. The production of recombinant nanobodies in Escherichia coli has been widely studied in shake flask cultures in rich medium. However, there are no upstream bioprocess studies of nanobody production in defined minimal medium and the effect of the induction
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Oxygen availability affects the synthesis of quorum sensing signal in the facultative anaerobe Novosphingobium pentaromativorans US6-1 Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-13 Yili Huang, Zejia Lu, Tingting Jiang, Yonghui Zeng, Yanhua Zeng, Baoliang Chen
Abstract Bacterial populations rely on quorum sensing (QS) to coordinate their behaviors and are often challenged by the fluctuation in oxygen concentrations in their habitats. Oxygen is a crucial factor that affects bacterial metabolism in multiple ways. However, little is known about whether and how oxygen availability affects QS activities. To fill this gap, we used the facultative anaerobe Novosphingobium
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Multifunctional cationic surfactants with a labile amide linker as efficient antifungal agents—mechanisms of action Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 E. Paluch, J. Szperlik, T. Czuj, M. Cal, Ł. Lamch, K. A. Wilk, E. Obłąk
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Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 Pengzhi Wang, Yi Yang, Tianqi Hong, Guoqiang Zhu
Abstract It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is developed for the sensitive protein detection and also can be used for the detection of pathogens. PLA employs aptamer or monoclonal/polyclonal
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New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 Veera Hämäläinen, Juan De Dios Barajas-López, Yana Berlina, Rafael Álvarez-Rafael, Klara Birikh
Abstract Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production. In all of these applications, the desired function of the endoglucanase is to create nicks in the amorphous regions of the cellulose. However, endoglucanase can be diverted
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Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC β-galactosidase Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 Daniel Mehabie Mulualem, Christy Agbavwe, Lesley A. Ogilvie, Brian V. Jones, Michelle Kilcoyne, Conor O’Byrne, Aoife Boyd
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Current status on the biodegradability of acrylic polymers: microorganisms, enzymes and metabolic pathways involved Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 Itzel Gaytán, Manuel Burelo, Herminia Loza-Tavera
Abstract Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most used AP is around 9 MT/year, which gives an idea of the amount of waste
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Effect of sample type and the use of high or low fishmeal diets on bacterial communities in the gastrointestinal tract of Penaeus monodon Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 T. H. Noble, H. H. Truong, A. Rombenso, C. J. Simon, N. M. Wade, J. W. Wynne
Abstract In shrimp aquaculture, manufactured diets that include various supplements and alternative fishmeal ingredients are increasingly being used and their effect on the gastrointestinal (GI) microbiota studied. However, dietary effects on different shrimp GI samples are not known. We investigated how a high (HFM) or low (LFM) fishmeal diet affects bacterial communities from different sample types
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Psychrophilic enzymes: structural adaptation, pharmaceutical and industrial applications Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-11 Sepideh Parvizpour, Nurulfarhana Hussin, Mohd Shahir Shamsir, Jafar Razmara
Abstract Psychrophiles are cold-living microorganisms synthesizing enzymes that are permanently active at almost near-zero temperatures. Psychrozymes are supposed to be structurally more flexible than their homologous proteins. This structural flexibility enables these proteins to undergo conformational changes during catalysis and improve catalytic efficiency at low temperatures. The outstanding characteristics
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Selection and characterization of a SpaCBA pilus-secreting food-grade derivative of Lacticaseibacillus rhamnosus GG Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-08 Hanne L. P. Tytgat, Pia Rasinkangas, Jarmo Ritari, Justus Reunanen, Steven Aalvink, Chia-wei Lin, Airi Palva, François P. Douillard, Willem M. de Vos
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Role of viral suppressors governing asymmetric synergism between tomato-infecting begomoviruses Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-08 Saumik Basu, Ashish Kumar Singh, Divya Singh, Sanjeeb Kumar Sahu, Supriya Chakraborty
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Recent advancements for microorganisms and their natural compounds useful in agriculture Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-08 Aurelio Ortiz, Estibaliz Sansinenea
Abstract During the past years, microorganisms have been the cause of many problems for human’s health. However, today with the development of many techniques of microbiology, the researchers have studied several roles of microorganisms which may help the society. Microbial-based products are expected to play important role in agriculture-enhancing plant production and therefore increasing crop’s yieldeswani
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Removal of pharmaceuticals by ammonia oxidizers during nitrification Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-07 Gang Wu, Jinju Geng, Ke Xu, Hongqiang Ren
Abstract The adverse effect of pharmaceuticals on ecosystem and human health raises great interest for the removal of pharmaceuticals in wastewater treatment plants (WWTPs). Enhanced removal of pharmaceuticals by ammonia oxidizers (AOs) has been observed during nitrification. This review provides a comprehensive summary on the removal of pharmaceuticals by AOs—ammonia oxidizing bacteria (AOB), ammonia
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Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-07 Hongli Wu, Takehito Nakazawa, Haibo Xu, Ruiheng Yang, Dapeng Bao, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda
Distinct wood degraders occupying their preferred habitats have biased enzyme repertoires that are well fitted to their colonized substrates. Pleurotus ostreatus, commonly found on wood, has evolved its own enzyme-producing traits. In our previous study, transcriptional shifts in several P. ostreatus delignification-defective mutants, including Δhir1 and Δgat1 strains, were analyzed, which revealed
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Bacterial association observations in Lucilia sericata and Lucilia cuprina organs through 16S rRNA gene sequencing Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-07 N. E. Gasz, M. J. Geary, S. L. Doggett, M. L. Harvey
Abstract Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debridement therapy (MDT) where the larvae ingest necrotic tissue and bacteria
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Application of electrical treatment on Euglena gracilis for increasing paramylon production Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-07 Jee Young Kim, Jeong-Joo Oh, Da Hee Kim, Hyun Soo Kim, Changsu Lee, Jaewon Park, Yoon-E Choi
Abstract Paramylon also called β-1,3-glucan is a value-added product produced from Euglena gracilis. Recently, researchers have developed various strategies for the enhanced paramylon production, among which electrical treatment for microbial stimulation can be an alternative owing to the applicability to large-scale cultivation. In this study, we applied the electrical treatment for enhanced paramylon
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A mitogen-activated protein kinase PoxMK1 mediates regulation of the production of plant-biomass-degrading enzymes, vegetative growth, and pigment biosynthesis in Penicillium oxalicum Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Bo Ma, Yuan-Ni Ning, Cheng-Xi Li, Di Tian, Hao Guo, Xiao-Ming Pang, Xue-Mei Luo, Shuai Zhao, Jia-Xun Feng
Mitogen-activated protein kinase (MAPK) cascades are broadly conserved and play essential roles in multiple cellular processes, including fungal development, pathogenicity, and secondary metabolism. Their function, however, also exhibits species and strain specificity. Penicillium oxalicum secretes plant-biomass-degrading enzymes (PBDEs) that contribute to the carbon cycle in the natural environment
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Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Barbora Branska, Maryna Vasylkivska, Hana Raschmanova, Katerina Jureckova, Karel Sedlar, Ivo Provaznik, Petra Patakova
Abstract Pumping toxic substances through a cytoplasmic membrane by protein transporters known as efflux pumps represents one bacterial mechanism involved in the stress response to the presence of toxic compounds. The active efflux might also take part in exporting low-molecular-weight alcohols produced by intrinsic cell metabolism; in the case of solventogenic clostridia, predominantly acetone, butanol
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Gut microbiome analysis as a predictive marker for the gastric cancer patients Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Yangyang Zhang, Jian Shen, Xinwei Shi, Yaoqiang Du, Yaofang Niu, Gulei Jin, Zhen Wang, Jianxin Lyu
Gut microbiota have been implicated in the development of cancer. Colorectal and gastric cancers, the major gastrointestinal tract cancers, are closely connected with the gut microbiome. Nevertheless, the characteristics of gut microbiota composition that correlate with gastric cancer are unclear. In this study, we investigated gut microbiota alterations during the progression of gastric cancer to
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Characterization of Bp GH16A of Bacteroides plebeius , a key enzyme initiating the depolymerization of agarose in the human gut Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Na Jung Park, Sora Yu, Dong Hyun Kim, Eun Ju Yun, Kyoung Heon Kim
Abstract Seaweeds have received considerable attention as sources of dietary fiber and biomass for manufacturing valuable products. The major polysaccharides of red seaweeds include agar and porphyran. In a marine environment, marine bacteria utilize agar and porphyran through the agarase and porphyranase genes encoded in their genomes. Most of these enzymes identified and characterized so far originate
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Adaptive laboratory evolution of methylotrophic Escherichia coli enables synthesis of all amino acids from methanol-derived carbon Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Jie Ren Gerald Har, Alec Agee, R. Kyle Bennett, Eleftherios T. Papoutsakis, Maciek R. Antoniewicz
Abstract Recent attempts to create synthetic Escherichia coli methylotrophs identified that de novo biosynthesis of amino acids, in the presence of methanol, presents significant challenges in achieving autonomous methylotrophic growth. Previously engineered methanol-dependent strains required co-utilization of stoichiometric amounts of co-substrates and methanol. As such, these strains could not be
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Gut microbiota affect the formation of calcium oxalate renal calculi caused by high daily tea consumption Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Feng Chen, Xuan Bao, Shiyu Liu, Kun Ye, Shasha Xiang, Liting Yu, Qingkang Xu, Yuehong Zhang, Xiu Wang, Xuan Zhu, Jian Ying, Yubiao Shen, Wei Ji, Shufeng Si
Abstract Kidney stones are a common and frequently occurring disease worldwide. Stones can cause urinary tract obstruction, pain, haematuria, and other symptoms. In this study, the relationship between calcium oxalate renal calculi and gut microbiota was considered. The dietary habits of 30 patients with calcium oxalate kidney stones and 30 healthy people were investigated. The 16S rDNA sequences and
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Bioinformatics: new tools and applications in life science and personalized medicine Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Iuliia Branco, Altino Choupina
Abstract While we have a basic understanding of the functioning of the gene when coding sequences of specific proteins, we feel the lack of information on the role that DNA has on specific diseases or functions of thousands of proteins that are produced. Bioinformatics combines the methods used in the collection, storage, identification, analysis, and correlation of this huge and complex information
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Tropical and temperate wastewater treatment plants assemble different and diverse microbiomes Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Yang Song, Wuttichai Mhuantong, Shuang-Yuan Liu, Nipon Pisutpaisal, Sarunyou Wongwilaiwalin, Pattanop Kanokratana, Ai-Jie Wang, Cheng-Ying Jiang, Verawat Champreda, Dong-Ru Qiu, Shuang-Jiang Liu
Abstract The diversity and assembly of activated sludge microbiomes play a key role in the performances of municipal wastewater treatment plants (WWTPs), which are the most widely applied biotechnological process systems. In this study, we investigated the microbiomes of municipal WWTPs in Bangkok, Wuhan, and Beijing that respectively represent tropical, subtropical, and temperate climate regions,
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Changes in planktonic and sediment bacterial communities under the highly regulated dam in the mid-part of the Three Gorges Reservoir Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Yu Qin, Qiong Tang, Lunhui Lu, Yuchun Wang, Irina Izaguirre, Zhe Li
Abstract Bacterial communities play an important role in the biogeochemical cycle in reservoir ecosystems. However, the dynamic changes in both planktonic and sediment bacterial communities in a highly regulated dam reservoir remain unclear. This study investigated the temporal distribution patterns of bacterial communities in a transition section of the Three Gorges Reservoir (TGR) using Illumina
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Thiamine: a key nutrient for yeasts during wine alcoholic fermentation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 PWJ Labuschagne, B Divol
Abstract Alcoholic fermentation is a crucial step of winemaking, during which yeasts convert sugars to alcohol and also produce or biotransform numerous flavour compounds. In this context, nutrients are essential compounds to support yeast growth and ultimately ensure complete fermentation, as well as optimized production of flavour compounds over that of off-flavour compounds. In particular, the vitamin
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Immunogenic characterization and protective efficacy of recombinant CsgA, major subunit of curli fibers, against Vibrio parahaemolyticus Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Sweta Karan, Devapriya Choudhury, Aparna Dixit
Abstract Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environment necessitate the development of safe and effective subunit vaccine against
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Isolation and screening of stress-resistant endophytic fungus strains from wild and cultivated soybeans in cold region of China Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Jia-lei Xiao, Jian-Guang Sun, Bo Pang, Xin Zhou, Yuan Gong, Lichao Jiang, Luan Zhang, Xiaodong Ding, Jing Yin
Abstract In this study, we firstly reported the large-scale screening and isolation of endophytic fungi from nine wild and six cultivated soybeans in the cold regions of China. We totally isolated 302 endophytic fungal strains, of which 215 strains are isolated from the wild soybeans and 87 are identified from cultivated soybeans. Among these endophytic fungal strains, in the roots, stems, and leaves
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Biotechnological strategies for the sustainable production of diosgenin from Dioscorea spp. Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Romaan Nazir, Vijay Kumar, Suphala Gupta, Padmanabh Dwivedi, Devendra Kumar Pandey, Abhijit Dey
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Biosynthesis and prebiotic activity of a linear levan from a new Paenibacillus isolate Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-06 Rui Cheng, Long Cheng, Yang Zhao, Lei Wang, Shiming Wang, Jianfa Zhang
Levan, a type of β (2→6)-linked fructan, is a promising biopolymer with distinct properties and extensive applications in the fields of food, pharmaceutical, cosmetics, etc. However, the commercial availability of levan is still limited due to the relatively high production costs. Here, a new Paenibacillus sp. strain FP01 was isolated and identified as an efficient fructan producer with high yield
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Skin tests for the detection of Mycobacterial infections: achievements, current perspectives, and implications for other diseases Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Malcolm S. Duthie, Steven G. Reed
Abstract Immunological and molecular advances have modernized diagnostic testing for many diseases. Although interferon gamma-release and polymerase chain reaction assays have been developed to detect Mycobacterium tuberculosis (Mtb) infection, purified protein derivative (PPD)–based tuberculin skin testing (TST) remains the most widely used method. Indeed, the TST is a simple and cost-effective tool
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Novel mycosynthesis of Co 3 O 4 , CuO, Fe 3 O 4 , NiO, and ZnO nanoparticles by the endophytic Aspergillus terreus and evaluation of their antioxidant and antimicrobial activities Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Shaimaa A. Mousa, El-Sayed R. El-Sayed, Samar S. Mohamed, Mohamed A. Abo El-Seoud, Adel A. Elmehlawy, Dalia A. M. Abdou
Abstract Several fungal endophytes were isolated and screened for their ability to biosynthesize a variety of nanoparticles (NPs), as a potentially simple and eco-friendly method with low cost. Among these fungi, a promising isolate named ORG-1 was found able to synthesize five different NPs types: Co3O4NPs, CuONPs, Fe3O4NPs, NiONPs, and ZnONPs. The ORG-1 strain was identified as Aspergillus terreus
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Involvement of phospholipase PLA 2 in production of cellulase and xylanase by Penicillium oxalicum Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Shi-Huan Li, Li-Sha Gu, Xiao-Yi Qu, Ting Zhang, Cheng-Xi Li, Rong-Ming Mai, Lu-Sheng Liao, Feng-Fei Zhang, Xue-Mei Luo, Shuai Zhao, Jia-Xun Feng
Abstract Phospholipases play vital roles in immune and inflammatory responses in mammals and plants; however, knowledge of phospholipase functions in fungi is limited. In this study, we investigated the effects of deleting predicted phospholipase genes on cellulase and xylanase production, and morphological phenotype, in Penicillium oxalicum. Individual deletion of nine of the ten predicted phospholipase
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From the vineyard to the cellar: new insights of Starmerella bacillaris (synonym Candida zemplinina ) technological properties and genomic perspective Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Wilson José Fernandes Lemos Junior, Vanessa Sales de Oliveira, Andre Fioravante Guerra, Alessio Giacomini, Viviana Corich
Abstract A large diversity of yeasts can be involved in alcoholic fermentation; however, Starmerella bacillaris strains have gained great attention due to their relevant and particular characteristics. S. bacillaris is commonly known as an osmotolerant, acidogenic, psychrotolerant, and fructophilic yeast. Most strains of this species are high producers of glycerol and show low ethanol production rates
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Efflux identification and engineering for ansamitocin P-3 production in Actinosynnema pretiosum Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Xinran Wang, Jianhua Wei, Yifan Xiao, Shuhui Luan, Xinjuan Ning, Linquan Bai
Abstract Ansamitocin P-3 (AP-3) exhibits potent biological activities against various tumor cells. As an important drug precursor, reliable supply of AP-3 is limited by low fermentation yield. Although different strategies have been implemented to improve AP-3 yield, few have investigated the impact of efflux on AP-3 production. In this study, AP-3 efflux genes were identified through combined analysis
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Natural bacterial isolates as an inexhaustible source of new bacteriocins Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Jelena Lozo, Ljubisa Topisirovic, Milan Kojic
Abstract Microorganisms isolated from various traditionally fermented food products prepared in households without commercial starter cultures are designated as natural isolates. In addition, this term is also used for microorganisms collected from various natural habitats or products (silage, soil, manure, plant and animal material, etc.) that do not contain any commercial starters or bacterial formulations
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Interactive effects of light quality and culturing temperature on algal cell size, biomass doubling time, protein content, and carbohydrate content Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Xiangpeng Li, Jacob Manuel, Shelyn Slavens, Daniel W. Crunkleton, Tyler W. Johannes
Abstract Light management strategy can be used to improve algal biomass and nutrient production. However, the response of algal metabolism to different light qualities, especially their interaction with other environmental factors, is not well understood. This study focuses on the interactive effects of light quality and culturing temperature on algal protein content and carbohydrate content of C.
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Progress of cationic gene delivery reagents for non-viral vector Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang
Gene delivery systems play a vital role in gene therapy and recombinant protein production. The advantages of using gene delivery reagents for non-viral vector include the capacity to accommodate a large packaging load and their low or absent immunogenicity. Furthermore, they are easy to produce at a large scale and preserve. Gene delivery reagents for non-viral vector are commonly used for transfecting
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Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Hengtao Tang, Xin Ju, Jing Zhao, Liangzhi Li
Abstract Ribose-5-phosphate isomerase B (RpiB) was first identified in the pentose phosphate pathway responsible for the inter-conversion of ribose-5-phosphate and ribulose-5-phosphate. Though there are seldom key enzymes in central carbon metabolic system developed as useful biocatalysts, RpiB with the advantages of wide substrate scope and high stereoselectivity has become a potential biotechnological
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Development and optimization of a pepino mosaic virus-based vector for rapid expression of heterologous proteins in plants Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Peter Abrahamian, John Hammond, Rosemarie W. Hammond
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Modeling the dynamic kinetics of microbial disinfection with dissipating chemical agents—a theoretical investigation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Micha Peleg
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Detection technologies and recent developments in the diagnosis of COVID-19 infection Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Praveen Rai, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit, Indrani Karunasagar, Iddya Karunasagar
Abstract COVID-19 is a disease caused by SARS-CoV-2 capable of causing mild to severe infections in humans. Since its first appearance in China in December 2019, the pandemic has spread rapidly throughout the world. Despite considerable efforts made to contain the disease, the virus has continued its prevalence in many countries with varying degrees of clinical manifestations. To contain this pandemic
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Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
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Streptomycetes as platform for biotechnological production processes of drugs Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Simona Barbuto Ferraiuolo, Marcella Cammarota, Chiara Schiraldi, Odile Francesca Restaino
Abstract Streptomyces is one of the most versatile genera for biotechnological applications, widely employed as platform in the production of drugs. Although streptomycetes have a complex life cycle and metabolism that would need multidisciplinary approaches, review papers have generally reported only studies on single aspects like the isolation of new strains and metabolites, morphology investigations
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Datamining and functional environmental genomics reassess the phylogenetics and functional diversity of fungal monosaccharide transporters Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Florian Barbi, Laurent Vallon, Carmen Guerrero-Galán, Sabine D. Zimmermann, Delphine Melayah, Danis Abrouk, Jeanne Doré, Marc Lemaire, Laurence Fraissinet-Tachet, Patricia Luis, Roland Marmeisse
Abstract Sugar transporters are essential components of carbon metabolism and have been extensively studied to control sugar uptake by yeasts and filamentous fungi used in fermentation processes. Based on published information on characterized fungal sugar porters, we show that this protein family encompasses phylogenetically distinct clades. While several clades encompass transporters that seemingly
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Biohybrid membranes for effective bacterial vehiculation and simultaneous removal of hexavalent chromium (CrVI) and phenol Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-04 Paola P. Pereira, Marilina Fernandez, Jonathan Cimadoro, Paola S. González, Gustavo M. Morales, Silvia Goyanes, Elizabeth Agostini
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Toxin-antitoxin HicAB regulates the formation of persister cells responsible for the acid stress resistance in Acetobacter pasteurianus Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-02 Kai Xia, Chengcheng Han, Jun Xu, Xinle Liang
Abstract Elucidation of the acetic acid resistance (AAR) mechanisms is of great significance to the development of industrial microbial species, specifically to the acetic acid bacteria (AAB) in vinegar industry. Currently, the role of population heterogeneity in the AAR of AAB is still unclear. In this study, we investigated the persister formation in AAB and the physiological role of HicAB in Acetobacter
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Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-02 Hongkai Liao, Haoxin Fan, Yaying Li, Huaiying Yao
Abstract Root-associated microorganisms play an important role in plant nutrition and productivity. However, our understanding of how a plant-microbiome system responds to pre-planting soil management remains limited. Here, continuous labeling with 13CO2 gas combined with stable isotope probing (SIP) was applied to explore bacterial utilization of plant-derived carbon (C) in the tomato rhizosphere
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A highly efficient protein degradation system in Bacillus sp. CN2: a functional-degradomics study Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-02 Yuhong Lai, Weiguang Li, Xiuyun Wu, Lushan Wang
Abstract A novel protease-producing Bacillus sp. CN2 isolated from chicken manure composts exhibited a relatively high proteolytic specific activity. The strain CN2 degradome consisted of at least 149 proteases and homolog candidates, which were distributed into 4 aspartic, 30 cysteine, 55 metallo, 56 serine, and 4 threonine proteases. Extracellular proteolytic activity was almost completely inhibited
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Catabolite responsive elements as a strategy for the control of heterologous gene expression in lactobacilli Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2020-11-20 Susana Langa, Ángela Peirotén, Juan Luis Arqués, José María Landete
Abstract Genes involved in the transport and catabolism of carbohydrates are usually controlled through the binding of the catabolite control protein A (CcpA) to the catabolite-responsive elements (cre) of target genes in Gram-positive bacteria. In this work, we show how the elimination of the cre sites in Lactobacillus casei BL23 promoters induced by sorbitol (PgutF), maltose (PmalL), and myo-inositol
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Supplementation of Bacillus sp. DU-106 reduces hypercholesterolemia and ameliorates gut dysbiosis in high-fat diet rats Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2020-10-31 Jianzhao Huang, Nan Xiao, Yiying Sun, Shanshan Wu, Wenni Tian, Yujian Lai, Pan Li, Bing Du
Abstract Gut microbiota modulation by a probiotic is a novel therapy for hypercholesterolemia mitigation. This study initially investigated the potential hypocholesterolemic effect of Bacillus sp. DU-106 in hypercholesterolemic rats and explored its potential relation with gut microbiota. Sprague–Dawley rats received a high-fat diet, or a high-fat diet supplemented with 7.5 × 109 and 1.5 × 1010 CFU/kg
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