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Semi-continuous production of xanthan in biofilm reactor using Xanthomonas campestris J. Biotechnol. (IF 3.503) Pub Date : 2021-01-13 Maryam Nejadmansouri; Maryam Razmjooei; Reza Safdarianghomsheh; Ehsan Shad; Frank Delvigne; Mohammadreza Khalesi
Semi-continuous production of xanthan gum using self-immobilized Xanthomonas campestris cells in biofilm reactors was studied. Fermentation was carried out using two different designs of biofilm reactor equipped with a) stainless-steel support (SSS) and b) polyethylene support (PES). Fermentation was performed in three cycles with refreshing the media at the beginning of each: cycle 1, 0−27 h; cycle
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Production of lipids by Chaetoceros affinis in media based on palm oil mill effluent J. Biotechnol. (IF 3.503) Pub Date : 2021-01-06 Karthick Murugan Palanisamy; Primilla Paramasivam; Gaanty Pragas Maniam; Mohd Hasbi Ab. Rahim; Natanamurugaraj Govindan; Yusuf Chisti
Biomass and lipid production by the marine diatom Chaetoceros affinis were characterized under continuous light with aeration. Media based on palm oil mill effluent (POME; 10, 20 and 30 % v/v in distilled water) were used together with a standard control medium. The maximum biomass concentration on day 12 of batch cultures in control medium was 821 ± 71 mg L–1. Under identical conditions, in the best
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Expression of Ice Recrystallization Inhibition protein in transgenic potato lines associated with reduced electrolyte leakage and efficient recovery post freezing injury J. Biotechnol. (IF 3.503) Pub Date : 2021-01-13 Khadija Aaliya; Idrees Ahmad Nasir; Anwar Khan; Nida Toufiq; Iqra Yousaf; Olawale Samuel Adeyinka; Sehrish Iftikhar; Abdul Munim Farooq; Bushra Tabassum
Potato (Solanum tuberosum L.) is considered to be frost-susceptible as short spells of frost can reduce the tuber yield and quality. Ice recrystallization inhibition (IRI) protein helps prevent growth of ice crystals in the cell apoplast during frost and help prevent damage associated with freezing stress. In this study, we investigated the in planta potential of Lolium perenne derived IRI3 transgene
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Investigating the pre-lethal cytotoxic effects of Bis(2,4-di-tert-butylphenyl)Phosphate on Chinese hamster ovary cells using high content analysis J. Biotechnol. (IF 3.503) Pub Date : 2021-01-13 Emma Harper; Yuling Xie; Lisa Connolly
Bis(2,4-di-tert-butylphenyl)Phosphate (bDtBPP) leaches out of polyethylene films used by the biopharmaceutical industry in single-use systems (SUS) for the culturing of drug producing cell lines. Previous studies found bDtBPP (0.025 – 0.110 mg/L) negatively affects Chinese hamster ovary (CHO) cell growth and productivity. Less information is known about the potential early stages of subtle pre-lethal
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Bacillus cereus liquid fertilizer was produced from Agaricus bisporus industrial wastewater J. Biotechnol. (IF 3.503) Pub Date : 2021-01-10 Jiafu Huang; Yizhen Zhuo; Jinqiu Lu; Qingyu Lai; Yawen Zhang
During the Agaricus bisporus canning processes, a large number of water-soluble elements were dissolved into the processing hot water. This study was conducted to use the industrial wastewater of A. bisporus to prepare agricultural microbial fertilizer. In the work, the influence of 6 different liquid fermentation factors on the total biomass of living Bacillus cereus was evaluated with the one-factor-at-a-time
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Biotechnological approaches to dissect climate-resilient traits in millets and their application in crop improvement J. Biotechnol. (IF 3.503) Pub Date : 2021-01-07 Roshan Kumar Singh; Mehanathan Muthamilarasan; Manoj Prasad
‘Small millets’ is a generic term that includes all the millets except pearl millet and sorghum. These small or minor millets constitute eleven species that are marginally cultivated and consumed worldwide. These small millets possess excellent agronomic-, climate-resilient, and nutritional traits, although they lack popularity. Small millets withstand a broad spectrum of environmental stresses and
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Protein cost allocation explains metabolic strategies in Escherichia coli J. Biotechnol. (IF 3.503) Pub Date : 2020-12-10 Pranas Grigaitis; Brett G. Olivier; Tomas Fiedler; Bas Teusink; Ursula Kummer; Nadine Veith
In-depth understanding of microbial growth is crucial for the development of new advances in biotechnology and for combating microbial pathogens. Condition-specific proteome expression is central to microbial physiology and growth. A multitude of processes are dependent on the protein expression, thus, whole-cell analysis of microbial metabolism using genome-scale metabolic models is an attractive
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Piriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus carica J. Biotechnol. (IF 3.503) Pub Date : 2020-12-30 Shahla Amani; Mehdi Mohebodini; Shahram Khademvatan; Morad Jafari; Vinod Kumar
Ficus carica L. is an important source of phenolic and flavonoid compounds with valuable pharmaceutical application across various diseases. The current study was carried out to investigate the influence of Piriformospora indica elicitation on growth, production of phenolic compounds, antioxidant capacity, and expression level of flavonoid biosynthetic pathway genes in hairy root (HR) cultures of F
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Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures J. Biotechnol. (IF 3.503) Pub Date : 2021-01-09 Simone Barera; Luca Dall’Osto; Roberto Bassi
Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contrasting reports have been published on the relation between photoprotective energy dissipation
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Selection and characterization of structure-switching DNA aptamers for the salivary peptide histatin 3 J. Biotechnol. (IF 3.503) Pub Date : 2020-12-30 Yagya R. Ojha; David R. Giovannucci; Brent D. Cameron
In this study, single-stranded DNA aptamers that switch structural conformation upon binding to the salivary peptide histatin 3 have been reported for the first time. Histatin 3 is an antimicrobial peptide that possesses the capability of being a therapeutic agent against oral candidiasis and has recently been linked as a novel biomarker for acute stress. The aptamers were identified through a library
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Bifunctional GM-CSF-derived peptides as tools for O-glycoengineering and protein tagging J. Biotechnol. (IF 3.503) Pub Date : 2020-12-30 María de los Milagros Sales; Ricardo Kratje; Marcos Oggero; Natalia Ceaglio
Rapid development of effective biotherapeutics has been a concern during the last couple decades. In our work we designed two novel peptide tags, GMOP and mGMOP, derived from the N-terminal region of human granulocyte and macrophage colony stimulating factor (hGM-CSF), which contain four and six potential O-glycosylation sites, respectively. These peptide tags were fused to the N-terminus of human
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Streptomyces griseus: A new biocatalyst with N-oxygenase activity J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Matías L. Nóbile; Abigail M. Stricker; Adolfo M. Iribarren; Elizabeth S. Lewkowicz
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Bacilysin within the Bacillus subtilis group: gene prevalence versus antagonistic activity against Gram-negative foodborne pathogens J. Biotechnol. (IF 3.503) Pub Date : 2020-12-30 Catherine Nannan; Huong Quynh Vu; Annika Gillis; Simon Caulier; Thuy Thanh Thi Nguyen; Jacques Mahillon
The Bacillus subtilis group comprises species known for their ability to produce a wide variety of antimicrobial peptides. This work focuses on bacilysin, a broad-spectrum active dipeptide, and its prevalence in the B. subtilis group. In silico genome analysis of strains from Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus licheniformis, Bacillus pumilus and B. subtilis subspecies inaquosorum
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A new synthetic biology approach for the production of curcumin and its glucoside in Atropa belladonna hairy roots J. Biotechnol. (IF 3.503) Pub Date : 2021-01-07 Sailendra Singh; Pallavi Pandey; Md. Qussen Akhtar; Arvind Singh Negi; Suchitra Banerjee
Curcumin has ignited global interest as an elite drugable molecule, owing to its time-honoured pharmacological activities against diverse human ailments. Limited natural accessibility and poor oral bioavailability caused major hurdles in the curcumin-based drug development process. We report the first successful testimony of curcumin and its glucoside synthesis in Atropa belladonna hairy roots (HR)
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Use of novel cystine analogs to decrease oxidative stress and control product quality J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Valentine Chevallier; Marvin Zoller; Nadine Kochanowski; Mikael R. Andersen; Christopher T. Workman; Laetitia Malphettes
Continuous improvements of cell culture media are required in order to ensure high yield and product quality. However, some components can be instable and lead to detrimental effects on bioprocess performances. l-cysteine is an essential amino acid commonly used in cell culture media. Despite its beneficial effect on recombinant protein production, in some cases, this component can be responsible for
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Transcriptome Analysis of Salt Stress Responsiveness in the Seedlings of Wild and Cultivated Ricinus communis L J. Biotechnol. (IF 3.503) Pub Date : 2021-01-06 Pei Lei; Zhi Liu; Yanbo Hu; HyokChol Kim; Shuo Liu; Jiaqi Liu; Liping Xu; Jianxin Li; Yong Zhao; Zhenliang Yu; Yanting Qu; Fenglang Huang; Fanjuan Meng
Soil salinity is one of the major environmental factors, influencing agricultural productivity of crops. As a non-edible and ideal oilseed crop, castor (Ricinus communis L.) has great industrial value in biofuel, but molecular mechanisms of salt stress regulation are still unknown. In this study, the differentially expressed genes (DEGs) for differential salt tolerance in two castor cultivar (wild
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Understanding the role of miRNAs for improvement of tea quality and stress tolerance J. Biotechnol. (IF 3.503) Pub Date : 2021-01-06 Debasish B. Krishnatreya; Niraj Agarwala; Sarvajeet Singh Gill; Tirthankar Bandyopadhyay
MicroRNAs (miRNAs) are an emerging class of small non-coding RNAs that exhibit important role in the regulation of gene expression, mostly through the mechanism of cleavage and/or inhibition of translation of the target mRNAs during or after transcription. Although much has been unravelled about the role of miRNAs in diverse biological processes like maintenance of functional integrity of genes and
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Biological validation of a novel process and product for quantitating western blots J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Thomas Diller; Jordan Thompson; Brian Steer
Protein normalization of western blots has relied upon housekeeping proteins which exhibit signal saturation and varied cellular expression level variations. These issues can produce spurious results leading to erroneous conclusions. A superior method to protein normalization using housekeeping proteins is Total Protein Normalization, a method now recognized as the gold standard for quantitative westerns
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The effect on single and combined stresses for biomass and lipid production from Nannochloris atomus using two phase culture system J. Biotechnol. (IF 3.503) Pub Date : 2020-12-29 So Hee Kim; Clovis Awah Che; Gwi-Taek Jeong; Sung-Koo Kim
The optimal conditions for high biomass and lipid production from Nannochloris atomus were evaluated. The parameters used in this study were light emitting diode (LED) wavelength mixing ratio, the photoperiod, salinity tolerance, and single and combined stresses. Biomass production was monitored in the first phase using red LED (625 nm), followed by lipid production by green LED (520 nm) in the second
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An engineered toluene dioxygenase for a single step biocatalytical production of (-)-(1S,2R)-cis-1,2-dihydro-1,2-naphthalenediol J. Biotechnol. (IF 3.503) Pub Date : 2020-12-28 Julian L. Wissner; Wendy Escobedo-Hinojosa; Andreas Vogel; Bernhard Hauer
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Production and antimicrobial activity of trans-resveratrol, trans-arachidin-1 and trans-arachidin-3 from elicited peanut hairy root cultures in shake flasks compared with bioreactors J. Biotechnol. (IF 3.503) Pub Date : 2020-12-28 Nichanan Eungsuwan; Phadtraphorn Chayjarung; Jintana Pankam; Vijakhana Pilaisangsuree; Pakwuan Wongshaya; Anupan Kongbangkerd; Chayaphon Sriphannam; Apinun Limmongkon
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Comparative genomic analysis reveals evolutionary and structural attributes of MCM gene family in Arabidopsis thaliana and Oryza sativa J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Sarvajeet Singh Gill; Priyanka Chahar; Anca Macovei; Sandeep Yadav; Abid A. Ansari; Narendra Tuteja; Ritu Gill
The mini-chromosome maintenance (MCM) family, a large and functionally diverse protein family belonging to the AAA + superfamily, is essential for DNA replication in all eukaryotic organisms. The MCM2-7 form a hetero-hexameric complex which serves as licensing factor necessary to ensure the proper genomic DNA replication during the S phase of cell cycle. MCM8-10 are also associated with the DNA replication
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Inducible plasmid display system for high-throughput selection of proteins with improved solubility J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Chakhee Kim; Myungseo Park; Jinkyeong Yang; Jonghyeok Shin; Yong-Cheol Park; Sun-Ki Kim; Dae-Hyuk Kweon
Soluble expression of enzymes inside the cell is a prerequisite for the successful biotransformation of valuable products. Some key enzymes involved in biotransformation processes, however, are hardly expressed in their soluble forms. Here, we propose an inducible plasmid display, which is a molecular evolution strategy coupled with a high-throughput screening and/or selection method, as a simple and
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Nanoparticle-based genetic transformation of Cannabis sativa J. Biotechnol. (IF 3.503) Pub Date : 2020-12-26 Sajjad Ahmed; Xuefei Gao; Md. Asraful Jahan; Maxwell Adams; Nianqiang Wu; Nik Kovinich
Cannabis sativa (Cannabis) is a multipurpose plant species consisting of specific lineages that for centuries has either been artificially selected for the production of fiber or the psychoactive drug Δ9-tetrahydrocannabinol (THC). With the recent lifting of previous legal restrictions on consuming Cannabis, there has been a resurgence of interest in understanding and manipulating Cannabis genetics
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Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells J. Biotechnol. (IF 3.503) Pub Date : 2020-12-07 Matthew Stuible; Christian Gervais; Simon Lord-Dufour; Sylvie Perret; Denis L’Abbé; Joseph Schrag; Gilles St-Laurent; Yves Durocher
Recombinant forms of the spike protein of SARS-CoV-2 and related viruses have proven difficult to produce with good yields in mammalian cells. Given the panoply of potential COVID-19 diagnostic tools and therapeutic candidates that require purified spike protein and its importance for ongoing SARS-CoV-2 research, we have explored new approaches for spike production and purification. Three transient
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Multi-assignment clustering: Machine learning from a biological perspective J. Biotechnol. (IF 3.503) Pub Date : 2020-12-04 Benjamin Ulfenborg; Alexander Karlsson; Maria Riveiro; Christian X. Andersson; Peter Sartipy; Jane Synnergren
A common approach for analyzing large-scale molecular data is to cluster objects sharing similar characteristics. This assumes that genes with highly similar expression profiles are likely participating in a common molecular process. Biological systems are extremely complex and challenging to understand, with proteins having multiple functions that sometimes need to be activated or expressed in a time-dependent
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A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling J. Biotechnol. (IF 3.503) Pub Date : 2020-12-07 Jennifer J. Labisch; Franziska Bollmann; Michael W. Wolff; Karl Pflanz
Lentiviral vectors have proven their great potential to serve as a DNA delivery tool for gene modified cell therapy and gene therapy applications. The downstream processing of these vectors is however still a great challenge, particularly because of the low stability of the virus. Harvesting and clarification are critical and until now insufficiently characterized steps for lentivirus processing. To
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Biocompatibility of magnetic nanoparticles coating with polycations using A549 cells J. Biotechnol. (IF 3.503) Pub Date : 2020-12-04 Elvira Rozhina; Anna Danilushkina; Farida Akhatova; Ramil Fakhrullin; Artem Rozhin; Svetlana Batasheva
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Flashing light emitting diodes (LEDs) induce proteins, polyunsaturated fatty acids and pigments in three microalgae J. Biotechnol. (IF 3.503) Pub Date : 2020-11-25 Serena Lima; Peter S.C. Schulze; Lisa M. Schüler; Ralf Rautenberger; Daniela Morales-Sánchez; Tamára F. Santos; Hugo Pereira; João C.S. Varela; Francesca Scargiali; René H. Wijffels; Viswanath Kiron
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Genome-wide identification and expression analysis of Arabidopsis GRAM-domain containing gene family in response to abiotic stresses and PGPR treatment J. Biotechnol. (IF 3.503) Pub Date : 2020-12-03 Shalini Tiwari; Mehanathan Muthamilarasan; Charu Lata
Characterization of stress-responsive genes is important to understand the genomics perspective of stress tolerance. In this purview, several gene-families are being identified and characterized in the model and non-model plant species, which has greatly enhanced the knowledge of molecular intricacies associated with stress tolerance. One such gene family is the GRAM-domain containing which have been
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Screening of endogenous strong promoters of Leuconostoc citreum EFEL2700 based on transcriptome analysis and its application for food-grade production of β-galactosidase J. Biotechnol. (IF 3.503) Pub Date : 2020-12-02 Seo Yeon Kim; Seul-Ah Kim; Ye-ji Jang; Seung-Oh Seo; Nam Soo Han
Leuconostoc citreum is a heterofermentative lactic acid bacterium frequently found in the various fermented foods. L. citreum EFEL2700 isolated from Korean kimchi has been used as a host strain for biotechnological applications. For the use as a food-grade host to over-produce food ingredients or enzymes, strong endogenous promoters guarantying high expression levels of target genes are necessary.
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Effect of red light on photosynthetic acclimation and the gene expression of certain light signalling components involved in the microRNA biogenesis in the extremophile Eutrema salsugineum J. Biotechnol. (IF 3.503) Pub Date : 2020-12-07 P. Pashkovskiy; S. Ryazansky; A. Kartashov; R. Voloshin; A. Khudyakova; A.A. Kosobryukhov; V.D. Kreslavski; Vl.V. Kuznetsov; S.I. Allakhverdiev
The photosynthetic acclimation of extremophile Eutrema salsugineum plants to red light (RL) (14 days, 150 µmol photons m-2 s-1, 660 nm) and the expression of the key photoreceptor apoprotein genes, transcription factors (TFs) and associated with phytochrome system MIR (microRNA) genes were studied. RL exposure induced an increase in the content of anthocyanin and total phenolic compounds and the level
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Recent progress in metabolic engineering of Saccharomyces cerevisiae for the production of malonyl-CoA derivatives J. Biotechnol. (IF 3.503) Pub Date : 2020-12-02 Shiyun Li; Qiyue Zhang; Jing Wang; Yingli Liu; Yunying Zhao; Yu Deng
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Genome-wide transcriptome variation landscape in Ruta chalepensis organs revealed potential genes responsible for rutin biosynthesis J. Biotechnol. (IF 3.503) Pub Date : 2020-11-30 Eslam M. Abdel-Salam; Mohammad Faisal; Abdulrahman A. Alatar; Ahmed A. Qahtan; Perwez Alam
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Capture of enzyme aggregates by covalent immobilization on solid supports. Relevant stabilization of enzymes by aggregation J. Biotechnol. (IF 3.503) Pub Date : 2020-11-26 Paz García-García; Gloria Fernandez-Lorente; Jose M. Guisan
In this paper, a novel procedure for the immobilization and stabilization of enzymes is proposed: the multipoint covalent attachment of bi-molecular enzyme aggregates. This immobilization protocol allows the “capture” and fixation of the enzyme aggregate on the support surface. In addition to stabilization by multipoint attachment, enzyme aggregation promotes very interesting stabilizing effects. In
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Harnessing High-throughput Phenotyping and Genotyping for Enhanced Drought Tolerance in Crop Plants J. Biotechnol. (IF 3.503) Pub Date : 2020-11-10 Javaid Akhter Bhat; Rupesh Deshmukh; Tuanjie Zhao; Gunvant Patil; Amit Deokar; Suhas Shinde; Juhi Chaudhary
Development of drought-tolerant cultivars is one of the challenging tasks for the plant breeders due to its complex inheritance and polygenic regulation. Evaluating genetic material for drought tolerance is a complex process due to its spatiotemporal interactions with environmental factors. The conventional breeding approaches are costly, lengthy, and inefficient to achieve the expected gain in drought
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The E3 ubiquitin ligase gene SlRING1 is essential for plant tolerance to cadmium stress in Solanum lycopersicum J. Biotechnol. (IF 3.503) Pub Date : 2020-11-10 Zhen-Yu Qi; Golam Jalal Ahammed; Chen-Yuan Jiang; Cai-Xia Li; Jie Zhou
The E3 ubiquitin ligases participate in the degradation of plant proteins and play a regulatory role in stress response. However, the role of tomato E3 ubiquitin ligase genes in plant response to heavy metal stress remains elusive. Here, we identified 17 tomato E3 ubiquitin ligase genes using blast analysis of highly expressed E3 ubiquitin ligase genes of Arabidopsis thaliana. Through organ expression
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Lectin-based assay for the determination of the inhibition activity of small molecule inhibitors of neuraminidases J. Biotechnol. (IF 3.503) Pub Date : 2020-11-18 Zuzana Hľasová; Lucia Pažitná; Miroslav Ondrejovič; Jaroslav Katrlík
Small molecules inhibitors of neuraminidases (NAs) are ones of the most prospective molecules proposed for the treatment of influenza viruses. The determination of their inhibition activity in vitro is an important step during the development of antiviral drugs. However, the analytical methods typically used for the evaluation of NA activity and inhibition (fluorescence-based assays using MUNANA substrate
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Pushing the limits: Cyclodextrin-based intensification of bioreductions J. Biotechnol. (IF 3.503) Pub Date : 2020-11-18 Christian Rapp; Bernd Nidetzky; Regina Kratzer
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Adhesion of fibroblast cells on thin films representing surfaces of polymeric scaffolds of human urethra rationalized by molecular models of integrin binding J. Biotechnol. (IF 3.503) Pub Date : 2020-11-04 Simona Braccini; Gianni Pecorini; Federica Chiellini; Dusan Bakos; Stanislav Miertus; Vladimir Frecer
This work combines experimental and computational study of Balb/3T3 clone A31 mouse embryo fibroblasts cell line adhesion and proliferation on fourteen different polymeric surfaces prepared from poly(dioxanone) (PDO), poly(glycolic acid) (PGA), poly(hydroxybutyrate) (PHB), and poly(L-lactic acid) (PLA), and their 1:1 mixtures. The study was done with the aim to explore the attractive interactions between
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Exogenous supplementation of melatonin alters representative organic acids and enzymes of respiratory cycle as well as sugar metabolism during arsenic stress in two contrasting indica rice cultivars J. Biotechnol. (IF 3.503) Pub Date : 2020-10-14 Santanu Samanta; Ankur Singh; Aditya Banerjee; Aryadeep Roychoudhury
The objective of the present investigation was to understand the impact of exogenously applied melatonin on mitochondrial respiration and sugar metabolism in two contrasting rice cultivars, viz., Khitish (arsenic-susceptible) and Muktashri (arsenic-tolerant) under arsenic-stress. Melatonin effectively restored the level of organic acids like pyruvic acid, malic acid and more particularly citric acid
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GABA reverses salt-inhibited photosynthetic and growth responses through its influence on NO-mediated nitrogen-sulfur assimilation and antioxidant system in wheat J. Biotechnol. (IF 3.503) Pub Date : 2020-11-12 Risheek Rahul Khanna; Badar Jahan; Noushina Iqbal; Nafees A. Khan; Mohamed F. AlAjmi; Md Tabish Rehman; M. Iqbal R. Khan
Gamma-aminobutyric acid (GABA) is a recently recognized signaling molecule participating in physiological processes, growth, and development of plants under optimal and stressful environments. In the present reported research, we investigated the role of GABA in imparting salt stress tolerance in wheat (Triticum aestivum L.). Exposure of wheat plants to 100 mM NaCl resulted in increased oxidative stress
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Glutathione improves rice tolerance to submergence: insights into its physiological and biochemical mechanisms J. Biotechnol. (IF 3.503) Pub Date : 2020-11-11 Nurealam Siddiqui; Mohammad Golam Mostofa; Mezanur Rahman; Tahjib-Ul-Arif; Ashim Kumar Das; Mohammed Mohi-Ud-Din; M. Motiar Rohman; Hafizur Rahman Hafiz; Mesbah Uddin Ansary; Lam-Son Phan Tran
Complete submergence (Sub) imposes detrimental effects on growth and survival of crop plants, including rice. Here, we investigated the beneficial effects of reduced glutathione (GSH) in mitigating Sub-induced adverse effects in two high-yielding rice cultivars BRRI dhan29 and dhan52. Both cultivars experienced growth defects, severe yellowing, necrosis and chlorosis, when they were completely immersed
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Sustainable synthesis of branched-chain diesters J. Biotechnol. (IF 3.503) Pub Date : 2020-11-11 Mar Serrano-Arnaldos; Salvadora Ortega-Requena; José Ángel Sánchez; Adrián Hernández; María Claudia Montiel; Fuensanta Máximo; Josefa Bastida
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Effects of metabolic pathway gene copy numbers on the biosynthesis of (2S)-naringenin in Saccharomyces cerevisiae J. Biotechnol. (IF 3.503) Pub Date : 2020-11-11 Hongbiao Li; Song Gao; Siqi Zhang; Weizhu Zeng; Jingwen Zhou
Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin biosynthetic pathway genes in S. cerevisiae. The efficiency of the naringenin metabolic
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Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin J. Biotechnol. (IF 3.503) Pub Date : 2020-11-10 Zahra Choolaei; Robert Flick; Anna N. Khusnutdinova; Elizabeth A. Edwards; Alexander F. Yakunin
Efficient biotransformation of lignin requires the activity of different oxidative enzymes. In this work, 19 bacterial multi-copper oxidases were screened for oxidase activity against 19 soluble substrates and revealed the highest activity in the laccase CotABsu (BSU0630) from Bacillus subtilis. Structure-based site-directed mutagenesis of CotABsu identified four conserved residues (His419, Cys492
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Magnesium oxide induces immunity against Fusarium wilt by triggering the jasmonic acid signaling pathway in tomato J. Biotechnol. (IF 3.503) Pub Date : 2020-11-10 Isamu Fujikawa; Yushi Takehara; Makiko Ota; Kiyoshi Imada; Kazunori Sasaki; Hiroshi Kajihara; Shoji Sakai; Sudisha Jogaiah; Shin-ichi Ito
Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (FOL), is a worldwide tomato disease. Although Fusarium wilt management remains unsuccessful, enhancing host FOL resistance using magnesium oxide to activate plant immunity may enable effective control. We demonstrated that MgO-pretreatment of roots induced FOL resistance in susceptible tomato plants. Resistance was not induced in tomato
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Nitric oxide: A radical molecule with potential biotechnological applications in fruit ripening J. Biotechnol. (IF 3.503) Pub Date : 2020-10-25 Francisco J. Corpas; Salvador González-Gordo; José M. Palma
Nitric oxide (NO) is a short-life and free radical molecule involved in a wide range of cellular, physiological and stressful processes in higher plants. In recent years it has been observed that exogenous NO application can palliate adverse damages against abiotic and biotic stresses. Conversely, there is accumulating information indicating that endogenous NO participates significantly in the mechanism
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NT*-HRV3CP: an optimized construct of human rhinovirus 14 3C protease for high-yield expression and fast affinity-tag cleavage J. Biotechnol. (IF 3.503) Pub Date : 2020-11-06 Elwy H. Abdelkader; Gottfried Otting
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Diversity analysis of ACC deaminase producing bacteria associated with rhizosphere of coconut tree (Cocos nucifera L.) grown in Lakshadweep islands of India and their ability to promote plant growth under saline conditions J. Biotechnol. (IF 3.503) Pub Date : 2020-10-23 Sangeeta Pandey; Shikha Gupta
ACC deaminase producing Plant growth promoting rhizobacteria (PGPR) offers a great promise for ameliorating the negative impacts of salinity stress manifested on plants. In this context, 28 rhizospheric bacteria associated with ACC deaminase potential (198−1069 nmol α-ketobutyrate mg protein−1 h−1) were isolated from 5 different islands of Lakshadweep, union territory, India- Agatti, Kavaratti, Bangaram
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Potassium: A key modulator for cell homeostasis J. Biotechnol. (IF 3.503) Pub Date : 2020-10-17 Prasann Kumar; Tapan Kumar; Simranjeet Singh; Narendra Tuteja; Ram Prasad; Joginder Singh
Potassium (K) is the most vital and abundant macro element for the overall growth of plants and its deficiency or, excess concentration results in many diseases in plants. It is involved in regulation of many crucial roles in plant development. Depending on soil-root interactions, complex soil dynamics often results in unpredictable availability of the elements. Based on the importance index, K is
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Role of Nano-Selenium in Health and Environment J. Biotechnol. (IF 3.503) Pub Date : 2020-11-04 Awanish Kumar; Kumar Suranjit Prasad
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O-Succinyl-L-homoserine overproduction with enhancement of the precursor succinyl-CoA supply by engineered Escherichia coli J. Biotechnol. (IF 3.503) Pub Date : 2020-11-04 Peng Liu; Ji-Song Liu; Wen-Yuan Zhu; Bo Zhang; Zhi-Qiang Liu; Yu-Guo Zheng
O-Succinyl-L-homoserine (OSH) is an important platform chemical in production of C4 chemicals such as succinic acid, homoserine lactone, γ‑butyrolactone, and 1,4‑butanediol. The production of OSH through chemical method or the current engineering strain is difficult and not optimal, and thereby there remains a need to develop new engineering strategy. Here, we engineered an OSH overproducing Escherichia
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Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae J. Biotechnol. (IF 3.503) Pub Date : 2020-11-03 Vera S. Efimova; Ludmila V. Isaeva; Philipp S. Orekhov; Marine E. Bozdaganyan; Mikhail A. Rubtsov; Ludmila A. Novikova
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Azospirillum brasilense reduces oxidative stress in the green microalgae Chlorella sorokiniana under different stressors J. Biotechnol. (IF 3.503) Pub Date : 2020-11-02 Haixin Peng; Luz E. de-Bashan; Brendan T. Higgins
In this study, we investigated oxidative stress in the green microalgae, Chlorella sorokiniana, in co-culture with the plant growth promoting bacteria (PGPB), Azospirillum brasilense. This relationship was studied in the absence of an exogenous stressor, under copper stress, and under nitrogen limitation stress. We confirmed that copper and nitrogen limitation induced algal oxidative stress and reductions
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Genetic regulation of water and nutrient transport in water stress tolerance in roots J. Biotechnol. (IF 3.503) Pub Date : 2020-10-07 Gloria Bárzana; Micaela Carvajal
Drought stress is one of the major abiotic factors affecting the growth and development of crops. The primary effect of drought is the alteration of water and nutrient uptake and transport by roots, related essentially with aquaporins and ion transporters of the plasma membrane. Therefore, the efficiency of water and nutrient transport across cell layers is a main factor in tolerance mechanisms. The
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Comprehensive proteomic analysis revealing multifaceted regulatory network of the xero-halophyte Haloxylon salicornicum involved in salt tolerance J. Biotechnol. (IF 3.503) Pub Date : 2020-10-15 Ashok Panda; Jaykumar Rangani; Asish Kumar Parida
Haloxylon salicornicum is a xero-halophyte which grow predominantly in dry saline areas. However, the proteomic approach for revealing the regulatory network involved in salt adaptation of this important xerohalophyte has not been studied so far. In the present investigation, the label-free quantitative proteomic analysis was carried out in shoot of H. salicornicum to get an insight into the functional
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Prospects for the creation of a waste-free technology for wastewater treatment and utilization of carbon dioxide based on cyanobacteria for biodiesel production J. Biotechnol. (IF 3.503) Pub Date : 2020-10-10 Kenzhegul Bolatkhan; Assem K. Sadvakasova; Bolatkhan K. Zayadan; Ardak B. Kakimova; Fariza K. Sarsekeyeva; Bekzhan D. Kossalbayev; Ayshat M. Bozieva; Saleh Alwasel; Suleyman I. Allakhverdiev
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Response to stress and allergen production caused by metal ions (Ni, Cu and Zn) in oregano (Origanum vulgare L.) plants J. Biotechnol. (IF 3.503) Pub Date : 2020-10-23 Kamila Kulbat-Warycha; Egli C. Georgiadou; Dorota Mańkowska; Beata Smolińska; Vasileios Fotopoulos; Joanna Leszczyńska
Heavy metals are the cause of one of the most significant biosphere contamination problems worldwide, as they can be highly reactive and toxic according to their oxidation levels. Their toxic effects are correlated with the elevated production of reactive oxygen species (ROS) and oxidative cellular damage occurring in plants. The aim of the present study was the investigation of the effects of three
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An NADH preferring acetoacetyl-CoA reductase is engaged in poly-3-hydroxybutyrate accumulation in Escherichia coli J. Biotechnol. (IF 3.503) Pub Date : 2020-10-26 Karel Olavarria; Alexandre Carnet; Joachim van Ranselaar; Caspar Quakkelaar; Ricardo Cabrera; Leonor Guedes da Silva; Aron L. Smids; Pablo Villalobos; Mark C.M. van Loosdrecht; S. Aljoscha Wahl
Oxygen supply implies higher production cost and reduction of maximum theoretical yields. Thus, generation of fermentation products is more cost-effective. Aiming to find a key piece for the production of (poly)-3-hydroxybutyrate (PHB) as a fermentation product, here we characterize an acetoacetyl-CoA reductase, isolated from a Candidatus Accumulibacter phosphatis-enriched mixed culture, showing a
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