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Shikonin Induces Glioma Necroptosis, Stemness Decline, and Impedes (Immuno)Proteasome Activity Stem Cells Int. (IF 4.3) Pub Date : 2024-3-14 Xianyun Qin, Lu Zhang, Jilan Liu, Yan Lu, Fuyao Zhou, Feng Jin
Gliomas, the most prevalent primary intracranial tumors, exhibit notable features such as heightened malignancy, rapid recurrence, and elevated mortality rates. Presently, standard therapeutic approaches yield limited curative outcomes. Shikonin, an extract derived from traditional Chinese medicine, demonstrates notable bioactivity against various tumors, including gliomas. This study elucidates Shikonin’s
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Stimulated Human Umbilical Cord Mesenchymal Stem Cells Enhance the Osteogenesis and Cranial Bone Regeneration through IL-32 Mediated P38 Signaling Pathway Stem Cells Int. (IF 4.3) Pub Date : 2024-3-13 Xiaru Zhang, Ying Zheng, Gang Wang, Yuanlin Liu, Yang Wang, Xueyi Jiang, Yueqing Liang, Xinfeng Zhao, Ping Li, Yi Zhang
Objective. Our previous study found that it could significantly increase the expression of IL32 after stimulating the human umbilical cord mesenchymal stem cells (S-HuMSCs). However, its role on the osteogenesis and cranial bone regeneration is still largely unknown. Here, we investigated the possible mechanism of this effect. Material and Methods. A series of experiments, including single-cell sequencing
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Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium Stem Cells Int. (IF 4.3) Pub Date : 2024-2-23 Shintarou Sakatoku, Yuki Hayashi, Taku Futenma, Yoshihiko Sugita, Ryo Ishizaka, Hiroyuki Nawa, Koichiro Iohara
Purpose. The microenvironment is required for tissues to maintain their properties in vivo. This microenvironment encompasses the types and three-dimensional arrangement of cells forming the tissues, and their interactions with neighboring cells and extracellular matrices, as represented by the stem cell niche. Tissue regeneration depends not on the original tissue source of the transplanted cells
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Different Levels of Autophagy Activity in Mesenchymal Stem Cells Are Involved in the Progression of Idiopathic Pulmonary Fibrosis Stem Cells Int. (IF 4.3) Pub Date : 2024-2-15 Hongxia Tao, Qin Lv, Jing Zhang, Lijuan Chen, Yang Yang, Wei Sun
Idiopathic pulmonary fibrosis (IPF) is an age-related lung interstitial disease that occurs predominantly in people over 65 years of age and for which there is a lack of effective therapeutic agents. It has demonstrated that mesenchymal stem cells (MSCs) including alveolar epithelial cells (AECs) can perform repair functions. However, MSCs lose their repair functions due to their distinctive aging
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Effects of Extracellular Vesicles Derived from Human Umbilical Cord Blood Mesenchymal Stem Cells on Cell Immunity in Nonobese Mice Stem Cells Int. (IF 4.3) Pub Date : 2024-2-2 Yang Ou, Yang Yang, Yan Wang, Heng Su, Yi-kun Zhou
Autoimmune responses are the most important pathogenic mechanisms underlying type 1 diabetes (T1D). Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have immunomodulatory effects. In this study, we investigated whether EVs derived from human umbilical cord MSCs (HucMSC-EVs) have treatment effects on nonobese diabetic (NOD) mice as model of T1D. HucMSC-EVs were isolated from human
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METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients Stem Cells Int. (IF 4.3) Pub Date : 2024-1-19 Xin Chen, Yuan Qin, Xian Wang, Hao Lei, Xiaochen Zhang, Houzhuo Luo, Changgang Guo, Weifu Sun, Shishu Fang, Wen Qin, Zuolin Jin
Objective. Periodontitis is a chronic inflammatory disease that causes loss of periodontal support tissue. Our objective was to investigate the mechanism by which METTL3-mediated N6-methyladenosine modification regulates the osteogenic differentiation through lncRNA in periodontal mesenchymal stem cells in patients with periodontitis (pPDLSCs). Material and Methods. We carried out a series of experiments
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The Neural Progenitor Cell-Associated Transcription Factor FoxG1 Regulates Cardiac Epicardial Cell Proliferation Stem Cells Int. (IF 4.3) Pub Date : 2024-1-11 Lucy Pilcher, Lara Solomon, Julie A. Dragon, Dhananjay Gupta, Jeffrey L. Spees
The epicardium is a layer of mesothelial cells that covers the surface of the heart. During development, epicardial cells undergo epithelial-to-mesenchymal transition (EMT) to form multipotent precursors that migrate into the heart and contribute to the coronary vasculature by differentiating into adventitial fibroblasts, smooth muscle cells, and endothelial cells. Epicardial cells also provide paracrine
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Development of an m6A-Related lncRNAs Signature Predicts Tumor Stemness and Prognosis for Low-Grade Glioma Patients Stem Cells Int. (IF 4.3) Pub Date : 2024-1-9 Dahua Xu, Peihu Li, Chunrui Zhang, Yutong Shen, Jiale Cai, Qingchen Wei, Meng Cao, Zhizhou Xu, Deng Wu, Hong Wang, Xiaoman Bi, Bo Wang, Kongning Li
Background. Growing evidence has revealed that m6A modification of long noncoding RNAs (lncRNAs) dynamically controls tumor stemness and tumorigenesis-related processes. However, the prognostic significance of m6A-related lncRNAs and their associations with stemness in low-grade glioma (LGG) remain to be clarified. Methods. A multicenter transcriptome analysis of lncRNA expression in 1,247 LGG samples
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Role of Angiogenesis and Its Biomarkers in Development of Targeted Tumor Therapies Stem Cells Int. (IF 4.3) Pub Date : 2024-1-4 Anchal Pathak, Ajay Kumar Pal, Subhadeep Roy, Mukesh Nandave, Keerti Jain
Angiogenesis plays a significant role in the human body, from wound healing to tumor progression. “Angiogenic switch” indicates a time-restricted event where the imbalance between pro- and antiangiogenic factors results in the transition from prevascular hyperplasia to outgrowing vascularized tumor, which eventually leads to the malignant cancer progression. In the last decade, molecular players, i
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Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid-Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis Stem Cells Int. (IF 4.3) Pub Date : 2024-1-3 Feiyue Zhao, Zhibin Fan, Ruikang Jia, Qichao Liu, Menglei Wang, Jianliang Sui, Huiyun Liu
Chronic gastric ulcer (CGU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Mesenchymal stem cells (MSCs) have been proven to have beneficial therapeutic effects in many human diseases. Here, a CGU model induced by acetic acid in mice was used to evaluate the repair effects and potential mechanism of human umbilical cord-derived MSCs (hUC-MSCs) and hUC-MSCs
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Adipose Tissue-Derived Extracellular Vesicles: A Promising Biomarker and Therapeutic Strategy for Metabolic Disorders Stem Cells Int. (IF 4.3) Pub Date : 2023-12-26 Wenhui Liu, Tianyan Liu, Qingyu Zhao, Junqiu Ma, Jiajia Jiang, Hui Shi
Adipose tissue plays an important role in systemic energy metabolism, and its dysfunction can lead to severe metabolic disorders. Various cells in adipose tissue communicate with each other to maintain metabolic homeostasis. Extracellular vesicles (EVs) are recognized as novel medium for remote intercellular communication by transferring various bioactive molecules from parental cells to distant target
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Combination Therapy with Platelet-Rich Plasma and Epidermal Neural Crest Stem Cells Increases Treatment Efficacy in Vascular Dementia Stem Cells Int. (IF 4.3) Pub Date : 2023-12-18 Somayeh Akbari, Masoud Haghani, Mojtaba Ghobadi, Etrat Hooshmandi, Afshin Borhani Haghighi, Mohammad Saied Salehi, Sareh Pandamooz, Negar Azarpira, Afsoon Afshari, Shahrbanoo Zabihi, Marzieh Nemati, Mahnaz Bayat
This study aimed to evaluate the efficacy and treatment mechanism of platelet-rich plasma (PRP) and neural crest-derived epidermal stem cells (ESCs) in their administration alone and combination in vascular dementia (VaD) model by two-vessel occlusion (2VO). Methods. Sixty-six rats were divided into six groups: the control, sham, 2VO + vehicle, 2VO + PRP, 2VO + ESC, and 2VO + ESC + PRP. The treated
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Advances and Challenges in Cancer Stem Cells for Onco-Therapeutics Stem Cells Int. (IF 4.3) Pub Date : 2023-12-6 Sulaiman Mohammed Alnasser
Six decades have passed since the foundational recognition of the primary properties of the stem cells. Research on stem cells has since remained at the forefront of efforts to combat a spectrum of diseases, most notably cancer. Despite remarkable progress in medical science, a definitive cure for cancer has remained elusive, spurring the pursuit of diverse therapeutic strategies, among which stem
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner In Vitro Stem Cells Int. (IF 4.3) Pub Date : 2023-11-30 Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach
First trimester (FTM) and term human umbilical cord perivascular cells are promising mesenchymal stromal cell candidates to mitigate side effects of oncotherapy, but their safety for cancer patients remains to be determined. This study was designed to determine if human umbilical cord perivascular cells modulate tumor growth when injected systemically in a tumor-bearing mouse model. Immunodeficient
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Immunization with Embryonic Stem Cells/Induced Pluripotent Stem Cells Induces Effective Immunity against Ovarian Tumor-Initiating Cells in Mice Stem Cells Int. (IF 4.3) Pub Date : 2023-11-27 Fengsheng Yu, Zujuan Zhang, Xiaohong Chang, Xue Ye, Hongyan Cheng, Yi Li, Heng Cui
Cancer stem cells (CSCs) express pluripotent markers and share many features with normal pluripotent stem cells. It is possible that immunity induced by embryonic stem cells (ESCs) and induced pluripotent stem cells- (IPSCs-) based vaccines may selectively target CSCs. In our study, cells expressing the pluripotent marker CD133 in the murine ovarian cancer cell-line ID8 were isolated and identified
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Unveiling Cellular Traits of Osteosarcoma Cancer Stem Cells via Bulk and Single-Cell RNA-Sequencing Analysis Stem Cells Int. (IF 4.3) Pub Date : 2023-11-22 Zhen Zhao, Shun Niu, Zhixiang Yu, Hongtao Zhang, Lizhuo Liang, Kui Xu, Chuan Dong, Hua Long
Osteosarcoma (OS) is a prototypical sarcoma, predominantly affecting adolescents. The hallmark of cancer stem cell (CSC) behavior pervades OS, invariably signifying an unfavorable prognosis. However, the intricacies underlying OS metastasis remain incompletely comprehended. As the frontiers of the scientific investigation push forward, encompassing both bulk sequencing and single-cell sequencing (scRNA)
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Mechanisms and Optimization Strategies of Paracrine Exosomes from Mesenchymal Stem Cells in Ischemic Heart Disease Stem Cells Int. (IF 4.3) Pub Date : 2023-11-22 Xiaorong Yin, Lizhi Lin, Fang Fang, Bin Zhang, Cheng Shen
The morbidity and mortality of myocardial infarction (MI) are increasing worldwide. Mesenchymal stem cells (MSCs) are multipotent stem cells with self-renewal and differentiation capabilities that are essential in tissue healing and regenerative medicine. However, the low implantation and survival rates of transplanted cells hinder the widespread clinical use of stem cells. Exosomes are naturally occurring
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Photobiomodulation Can Enhance Stem Cell Viability in Cochlea with Auditory Neuropathy but Does Not Restore Hearing Stem Cells Int. (IF 4.3) Pub Date : 2023-11-15 So-Young Chang, Eunjeong Kim, Nathaniel T. Carpena, Jae-Hun Lee, Doo Hee Kim, Min Young Lee
Sensorineural hearing loss is very difficult to treat. Currently, one of the techniques used for hearing rehabilitation is a cochlear implant that can transform sound into electrical signals instead of inner ear hair cells. However, the prognosis remains very poor if sufficient auditory nerve cells are not secured. In this study, the effect of mouse embryonic stem cells (mESC) and photobiomodulation
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Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation Stem Cells Int. (IF 4.3) Pub Date : 2023-11-14 Qiongqiong Gao, Zhentao Gao, Minzhi Su, Yong Huang, Chi Zhang, Cuiping Li, Hailun Zhan, Bolong Liu, Xiangfu Zhou
Interstitial cystitis/bladder pain syndrome (IC/BPS) seriously reduces the patient’s quality of life, yet current therapies only provide partial relief. In the spinal dorsal horn (SDH), neuroinflammation plays a pivotal role in the development of IC. Injection of human umbilical cord mesenchymal stem cells (hUMSCs) to reduce inflammation is an effective strategy, and heme oxygenase-1 (HO-1) exhibits
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CRISPR/Cas9 Genome Editing in LGMD2A/R1 Patient-Derived Induced Pluripotent Stem and Skeletal Muscle Progenitor Cells Stem Cells Int. (IF 4.3) Pub Date : 2023-11-9 Lampros Mavrommatis, Abdul Zaben, Urs Kindler, Marie-Cécile Kienitz, Julienne Dietz, Hyun-Woo Jeong, Pierre Böhme, Beate Brand-Saberi, Matthias Vorgerd, Holm Zaehres
Large numbers of Calpain 3 (CAPN3) mutations cause recessive forms of limb-girdle muscular dystrophy (LGMD2A/LGMDR1) with selective atrophy of the proximal limb muscles. We have generated induced pluripotent stem cells (iPSC) from a patient with two mutations in exon 3 and exon 4 at the calpain 3 locus (W130C, 550delA). Two different strategies to rescue these mutations are devised: (i) on the level
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Adipose-Derived Mesenchymal Stem Cell Facilitate Hematopoietic Stem Cell Proliferation via the Jagged-1/Notch-1/Hes Signaling Pathway Stem Cells Int. (IF 4.3) Pub Date : 2023-11-9 Hongbo Wang, Xiaojuan Bi, Rongyao Zhang, Hailong Yuan, Jianli Xu, Kaile Zhang, Songqing Qi, Xue Zhang, Ming Jiang
Background. Poor graft function (PGF) is a life-threatening complication following hematopoietic stem cell transplantation (HSCT). Current therapies, such as CD34+ cell infusion, have shown limited effectiveness. Conversely, mesenchymal stem cells (MSCs) show potential in addressing PGF. Adipose-derived mesenchymal stem cells (ADSCs) effectively support long-term hematopoietic stem cell proliferation
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Stem Cell-Based Therapy and Cell-Free Therapy as an Alternative Approach for Cardiac Regeneration Stem Cells Int. (IF 4.3) Pub Date : 2023-11-2 Iwona Deszcz
The World Health Organization reports that cardiovascular diseases (CVDs) represent 32% of all global deaths. The ineffectiveness of conventional therapies in CVDs encourages the development of novel, minimally invasive therapeutic strategies for the healing and regeneration of damaged tissue. The self-renewal capacity, multilineage differentiation, lack of immunogenicity, and immunosuppressive properties
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Curative Effect of AD-MSCs against Cisplatin-Induced Hepatotoxicity in Rats is Potentiated by Azilsartan: Targeting Oxidative Stress, MAPK, and Apoptosis Signaling Pathways Stem Cells Int. (IF 4.3) Pub Date : 2023-10-23 Amany Abdlrehim Bekhit, Olivia N. Beshay, Michael A. Fawzy, Sara Mohamed Naguib Abdel-Hafez, Gaber El-Saber Batiha, Farid S. Ataya, Moustafa Fathy
Despite its clinical value, cisplatin (CISP) is complicated by marked hepatotoxicity via inducing oxidative stress, inflammatory, and apoptotic pathways. This study aims to explore the protective impact of azilsartan (AZIL), an antihypertensive drug, in addition to adipose tissue-derived mesenchymal stem cells (AD-MSCs) on CISP-induced hepatotoxicity. After characterization and labeling of AD-MSCs
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Transglutaminase 2 Prevents Premature Senescence and Promotes Osteoblastic Differentiation of Mesenchymal Stem Cells through NRF2 Activation Stem Cells Int. (IF 4.3) Pub Date : 2023-10-20 Soo-Jin Lee, Ji-Woong Shin, Mee-Ae Kwon, Ki Baek Lee, Hyo-Jun Kim, Jin-Haeng Lee, Heun-Soo Kang, Jong Kwan Jun, Sung-Yup Cho, In-Gyu Kim
Transglutaminase 2 (TG2) is a multifunctional enzyme that exhibits transamidase, GTPase, kinase, and protein disulfide isomerase (PDI) activities. Of these, transamidase-mediated modification of proteins regulates apoptosis, differentiation, inflammation, and fibrosis. TG2 is highly expressed in mesenchymal stem cells (MSCs) compared with differentiated cells, suggesting a role of TG2 specific for
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Umbilical Cord-Derived Mesenchymal Stem Cells Attenuate S100-Induced Autoimmune Hepatitis via Modulating Th1 and Th17 Cell Responses in Mice Stem Cells Int. (IF 4.3) Pub Date : 2023-10-17 Xiaofeng Wei, Xinhong Cheng, Yang Luo, Xun Li
Currently, the first-line treatment for autoimmune hepatitis (AIH) is still the combination of glucocorticoids or immunosuppressants. However, hormone and immunosuppressive therapy can cause serious side effects, such as Cushing syndrome and bone marrow suppression. Previous studies reported on the applicability and safety of mesenchymal stem cells (MSCs) to ameliorate liver inflammation and fibrosis
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VCAM-1 Promotes Angiogenesis of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Trauma-Induced Osteonecrosis of the Femoral Head by Regulating the Apelin/CCN2 Pathway Stem Cells Int. (IF 4.3) Pub Date : 2023-10-12 Yiming Shao, Lei Sun, Baodong Ma, Ranran Jin, Yueyao Ban, Ruibo Li, Jianfa Wang, Hongkai Lian, Han Yue
Trauma-induced osteonecrosis of the femoral head (TI-ONFH) is a pathological process in which the destruction of blood vessels supplying blood to the femoral head causes the death of bone tissue cells. Vascular cell adhesion molecule 1 (VCAM-1) has been shown to have potent proangiogenic activity, but the role in angiogenesis of TI-ONFH is unclear. In this work, we discovered that VCAM-1 was significantly
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Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review Stem Cells Int. (IF 4.3) Pub Date : 2023-10-11 Xiaolei Bai, Ruijue Cao, Danni Wu, Huicong Zhang, Fan Yang, Linhong Wang
Bone tissue engineering (BTE) is a promising approach for repairing and regenerating damaged bone tissue, using stem cells and scaffold structures. Among various stem cell sources, dental pulp stem cells (DPSCs) have emerged as a potential candidate due to their multipotential capabilities, ability to undergo osteogenic differentiation, low immunogenicity, and ease of isolation. This article reviews
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Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells Stem Cells Int. (IF 4.3) Pub Date : 2023-9-29 Xuerui Yao, Ji Hyun Kang, Kee-Pyo Kim, Hyogeun Shin, Zhe-Long Jin, Hao Guo, Yong-Nan Xu, Ying-Hua Li, Sai Hali, Jeongwoo Kwon, Hyeonwoo La, Chanhyeok Park, Yong-June Kim, Lin Wang, Kwonho Hong, Qilong Cao, Il-Joo Cho, Nam-Hyung Kim, Dong Wook Han
Brain organoids have been considered as an advanced platform for in vitro disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoid technology. Here, we describe the high-speed and large-scale production of midbrain organoids using
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Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/β-Catenin Pathway Stem Cells Int. (IF 4.3) Pub Date : 2023-9-27 Xin Tang, Cuixiang Cao, Yunxiao Liang, Le Han, Bin Tu, Miao Yu, Miaojian Wan
The most prevalent type of alopecia is androgenetic alopecia (AGA), which has a high prevalence but no effective treatment. Elevated dihydrotestosterone (DHT) level in the balding area was usually thought to be critical in the pathophysiology of AGA. The canonical Wnt/β-catenin signaling pathway plays a key role in promoting hair follicle development and sustaining the hair follicle cycle. Adipose-derived
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Human Mesenchymal Stem Cells-Derived Exosome Mimetic Vesicles Regulation of the MAPK Pathway and ROS Levels Inhibits Glucocorticoid-Induced Apoptosis in Osteoblasts Stem Cells Int. (IF 4.3) Pub Date : 2023-9-20 Hongxu Lu, Zhaoxia Zhang, Zhaoying Wang, Jinkui Wang, Tao Mi, Liming Jin, Xin Wu, Junyi Luo, Yimeng Liu, Junhong Liu, Wenquan Cai, Peng Guo, Dawei He
Background. Long-term extensive use of glucocorticoids will lead to hormonal necrosis of the femoral head, and osteoblasts play an important role in the prevention of osteonecrosis. However, there is no complete cure for necrosis of the femoral head. Mesenchymal stem cell- (MSCs-) derived exosomes are widely used for the repair of various tissue lesions. Therefore, the aim of this study was to investigate
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Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties Stem Cells Int. (IF 4.3) Pub Date : 2023-9-20 Agnieszka Smolinska, Aleksandra Bzinkowska, Paulina Rybkowska, Magdalena Chodkowska, Anna Sarnowska
The heterogeneity of the mesenchymal stem/stromal cells (MSCs) population poses a challenge to researchers and clinicians, especially those observed at the population level. What is more, the lack of precise evidences regarding MSCs developmental origin even further complicate this issue. As the available evidences indicate several possible pathways of MSCs formation, this diverse origin may be reflected
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The Functions and Mechanisms of Tendon Stem/Progenitor Cells in Tendon Healing Stem Cells Int. (IF 4.3) Pub Date : 2023-9-12 Jingwei Lu, Hui Chen, Kexin Lyu, Li Jiang, Yixuan Chen, Longhai Long, Xiaoqiang Wang, Houyin Shi, Sen Li
Tendon injury is one of the prevalent disorders of the musculoskeletal system in orthopedics and is characterized by pain and limitation of joint function. Due to the difficulty of spontaneous tendon healing, and the scar tissue and low mechanical properties that usually develops after healing. Therefore, the healing of tendon injury remains a clinical challenge. Although there are a multitude of approaches
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Retracted: Severity Assessment of COVID-19 Using a CT-Based Radiomics Model. Stem Cells Int. (IF 4.3) Pub Date : 2023-09-14 Stem Cells International
[This retracts the article DOI: 10.1155/2021/2263469.].
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Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung Stem Cells Int. (IF 4.3) Pub Date : 2023-9-5 Alejandra Hernandez Pichardo, Bettina Wilm, Neill J. Liptrott, Patricia Murray
Mesenchymal stromal cells (MSCs) administered intravenously (IV) have shown efficacy in preclinical models of various diseases. This is despite the cells not reaching the site of injury due to entrapment in the lungs. The immunomodulatory properties of MSCs are thought to underlie their therapeutic effects, irrespective of whether they are sourced from bone marrow, adipose tissue, or umbilical cord
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Chromosomal Instability in Various Generations of Human Mesenchymal Stem Cells Following the Therapeutic Radiation Stem Cells Int. (IF 4.3) Pub Date : 2023-8-29 Majid Sadeghi Moghadam, Hosein Azimian, Jalil Tavakol Afshari, Mohammad Taghi Bahreyni Toossi, Najmeh Kaffash Farkhad, Seyed Hamid Aghaee-Bakhtiari
Background. Radiotherapy is a crucial treatment for most malignancies. However, it can cause several side effects, including the development of secondary malignancies due to radiation-induced genomic instability (RIGI). The aim of this study was to evaluate genomic instability in human mesenchymal stem cells (hMSCs) at different X-ray radiation doses. Additionally, the study aimed to examine the relative
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Use of Multiple Doses of Intravenous Infusion of Umbilical Cord-Mesenchymal Stem Cells for the Treatment of Adult Patients with Severe COVID-19-Related Acute Respiratory Distress Syndrome: Literature Review Stem Cells Int. (IF 4.3) Pub Date : 2023-8-18 Po-Ren Hsueh, Sung-Jung Ho, Po-Chuen Hsieh, I-Min Liu, Shio-Shin Jean
Objectives. Acute respiratory distress syndrome (ARDS) is a critical complication in severe COVID-19 patients. The intravenous infusion (IVF) of umbilical cord- (UC-) mesenchymal stem cells (MSCs), validated to substantially reduce the release of several inflammatory cytokines in vivo, was also shown to exhibit benefits in improving hypoxemia among severe COVID-19 patients. A single dose of IVF-UC-MSCs
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Identification of Functional Modules and Key Pathways Associated with Innervation in Graft Bone—CGRP Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via p38 MAPK and Wnt6/β-Catenin Stem Cells Int. (IF 4.3) Pub Date : 2023-8-16 Ziqian Wu, Xudong Wang, Jingcun Shi, Anand Gupta, Yuhan Zhang, Bingqing Zhang, Yang Cao, Lei Wang
Bone resorption occurs after bone grafting, however, contemporaneous reconstruction of the innervation of the bone graft is a potential treatment to maintain the bone mass of the graft. The innervation of bone is an emerging research topic. To understand the potential molecular mechanisms of bone innervation after bone grafting, we collected normal iliac bone tissue as well as bone grafts with or without
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miR-4780 Derived from N2-Like Neutrophil Exosome Aggravates Epithelial-Mesenchymal Transition and Angiogenesis in Colorectal Cancer Stem Cells Int. (IF 4.3) Pub Date : 2023-8-4 Liang Wang, Yuqiang Shan, Sixin Zheng, Jiangtao Li, Peng Cui
Despite significant advances in diagnostic methods and treatment strategies, the prognosis for patients with advanced colon cancer remains poor, and mortality rates are often high due to metastasis. Increasing evidence showed that it is of significant importance to investigate how the tumor microenvironment participates in the development of colorectal cancer (CRC). In this manuscript, neutrophils
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The Effect of Mesenchymal Stem Cell-Derived Exosomes and miR17-5p Inhibitor on Multicellular Liver Fibrosis Microtissues Stem Cells Int. (IF 4.3) Pub Date : 2023-8-4 Farnaz Sani, Mina Soufi Zomorrod, Negar Azarpira, Masoud Soleimani
Background. Although several studies have been conducted on modeling human liver disease, it is still challenging to mimic nonalcoholic fatty liver disease in vitro. Here, we aimed to develop a fibrotic liver microtissue composed of hepatocytes, hepatic stellate, and endothelial cells. In addition, the therapeutic effects of umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-EXO) and anti-miR17-5p
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Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia Stem Cells Int. (IF 4.3) Pub Date : 2023-8-1 Ying Chen, Chenchen Zhou, Xiaobo Zhao, Ronghua Che, Yuelin Wu, Sheng Wan, Jinda Pei, Liping Yao, Xiaolin Hua
Preeclampsia is a pregnancy disorder characterized by systemic organ damage and high blood pressure. It has been reported that microRNA-195 (miR-195) is associated with preeclampsia. In this study, we discovered the target of miR-195 in regulating human extravillous cytotrophoblast-derived transformed cell proliferation and migration. We analyzed the clinicopathological factors of preeclampsia and
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Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice Stem Cells Int. (IF 4.3) Pub Date : 2023-7-28 Shutaro Habata, Ramanaiah Mamillapalli, Abdullah Ucar, Hugh S. Taylor
Endometriosis is a chronic inflammatory gynecological disorder regulated by estrogen and characterized by the growth of endometrial tissue outside the uterus. We have previously demonstrated that mesenchymal stem cells (MSCs) contribute directly to endometriosis. Here, we investigated an indirect effect; we hypothesized that MSCs may also impact the bone marrow (BM) by regulating bone marrow-derived
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Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1α Branch of the Endoplasmic Reticulum Stress Response Stem Cells Int. (IF 4.3) Pub Date : 2023-7-26 Ruixi Luo, Yaqiong Wei, Peng Chen, Jing Zhang, La Wang, Wenjia Wang, Ping Wang, Weiyi Tian
Background. Idiopathic pulmonary fibrosis (IPF) is the most common idiopathic interstitial lung disease, and it carries a poor prognosis due to a lack of efficient diagnosis methods and treatments. Epithelial-mesenchymal transition (EMT) plays a key role in IPF pathogenesis. Endoplasmic reticulum (ER) stress contributes to fibrosis via EMT-mediated pathways. Mesenchymal stem cell (MSC) transplantation
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The m6A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway Stem Cells Int. (IF 4.3) Pub Date : 2023-7-25 Xiang Gao, Jian Wang, Yibo Wang, Weixu Li, Zhijun Pan
N6-methyladenosine (m6A) mRNA methylation has emerged as an important player in many biological processes by regulating gene expression. As a crucial reader, YTHDF1 usually improves the translation efficiency of its target mRNAs. However, its roles in bone marrow mesenchymal stem cells (BMSCs) osteogenesis remain largely unknown. Here, we reported that YTHDF1, an m6A reader, is highly expressed during
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Abnormal Decrease of Macrophage ALKBH5 Expression Causes Abnormal Polarization and Inhibits Osteoblast Differentiation Stem Cells Int. (IF 4.3) Pub Date : 2023-7-19 Junquan Weng, Haidong Fan, Huijuan Liu, Su Tang, Yuyan Zheng
Peri-implant tissue inflammation is an inflammatory injury that occurs in the soft and hard tissues surrounding the implant and is the main cause of short- or long-term failure of implant prosthetic restorations, which is compounded by bone loss and bone destruction in the alveolar bone of diabetes patients with peri-implantitis. However, the mechanisms underlying the persistence of diabetic peri-implantitis
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Human Umbilical Mesenchymal Stem Cells-Derived Microvesicles Attenuate Formation of Hypertrophic Scar through Multiple Mechanisms Stem Cells Int. (IF 4.3) Pub Date : 2023-7-15 Qun Qian, Ni Zhu, Wenzhe Li, Songlin Wan, Dongcheng Wu, Yunhua Wu, Weicheng Liu
Mesenchymal stem cells and the derived extracellular microvesicles are potential promising therapy for many disease conditions, including wound healing. Since current therapeutic approaches do not satisfactorily attenuate or ameliorate formation of hypertrophic scars, it is necessary to develop novel drugs to achieve better outcomes. In this study, we investigated the effects and the underlying mechanisms
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Galectin-3 Plays an Important Role in BMP7-Induced Cementoblastic Differentiation of Human Periodontal Ligament Cells by Interacting with Extracellular Components Stem Cells Int. (IF 4.3) Pub Date : 2023-6-23 Min-Jeong Choi, Tae Min You, Young-Joo Jang
Human periodontal ligament stem cells (hPDLSCs) contain multipotent postnatal stem cells that differentiate into PDL progenitors, osteoblasts, and cementoblasts. Previously, we obtained cementoblast-like cells from hPDLSCs using bone morphogenetic protein 7 (BMP7) treatment. Differentiation into appropriate progenitor cells requires interactions and changes between stem or progenitor cells and their
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Comparison of Biological Properties and Clinical Application of Mesenchymal Stem Cells from the Mesoderm and Ectoderm Stem Cells Int. (IF 4.3) Pub Date : 2023-6-10 Zhenning Wang, Meng Huang, Yu Zhang, Xiaoxia Jiang, Lulu Xu
Since the discovery of mesenchymal stem cells (MSCs) in the 1970s, they have been widely used in the treatment of a variety of diseases because of their wide sources, strong differentiation potential, rapid expansion in vitro, low immunogenicity, and so on. At present, most of the related research is on mesoderm-derived MSCs (M-MSCs) such as bone marrow MSCs and adipose-derived MSCs. As a type of MSC
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METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation Stem Cells Int. (IF 4.3) Pub Date : 2023-6-7 Xiao Han, Guoyue Li, Haoqing Yang, Chen Zhang, Yangyang Cao, Ning Wang, Lihua Ge, Zhipeng Fan
Mesenchymal stem cells (MSCs) have been considered a potential method for the regeneration of tooth and maxillofacial bone defects based on the multidirectional differentiation characteristics of MSCs. miRNAs have been found to play a key role in the differentiation of MSCs. However, its effectiveness still needs to be improved, and its internal mechanism is still unclear. In the present study, our
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MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines Stem Cells Int. (IF 4.3) Pub Date : 2023-6-2 Xueheng Zhao, Zenghui Huang, Yongzhe Chen, Qianyin Zhou, Fang Zhu, Huan Zhang, Dai Zhou
Spermatogonial stem cells are committed to initiating and maintaining male spermatogenesis, which is the foundation of male fertility. Understanding the mechanisms underlying SSC fate decisions is critical for controlling spermatogenesis and male fertility. However, the key molecules and mechanisms responsible for regulating human SSC development are not clearly understood. Here, we analyzed normal
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SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular Quiescence Stem Cells Int. (IF 4.3) Pub Date : 2023-5-27 Yuehong Wang, Yuman Yu, Weijun Yang, Linying Wu, Yaoshun Yang, Qianyun Lu, Jianying Zhou
Increasing evidence indicates that quiescent cancer stem cells (CSCs) are a root cause of chemoresistance. SET domain-containing protein 4 (SETD4) epigenetically regulates cell quiescence in breast cancer stem cells (BCSCs), and SETD4-positive BCSCs are chemoradioresistant. However, the role of SETD4 in chemoresistance, tumor progression, and prognosis in nonsmall cell lung cancer (NSCLC) patients
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Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway Stem Cells Int. (IF 4.3) Pub Date : 2023-5-26 Min Liu, Rui Chen, Yunxuan Xu, Jiawen Zheng, Min Wang, Ping Wang
Human periodontal ligament stem cells (PDLSCs) are the most promising stem cells for periodontal tissue engineering. Senescent PDLSCs have diminished abilities to proliferate and differentiate, affecting the efficiency of periodontal tissue repair and regeneration. Stem cell-derived exosomes are important participants in intercellular information exchange and can help ameliorate senescence. In this
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Bone Marrow-Derived Mesenchymal Stem Cells and γ-Secretase Inhibitor Treatments Suppress Amyloid-β25–35-Induced Cognitive Impairment in Rat Dams and Cortical Degeneration in Offspring Stem Cells Int. (IF 4.3) Pub Date : 2023-5-25 Asmaa Gaber, Ahlam M. Elbakry, Rabab M. Aljarari, Fatima A. Jaber, Yasser A. Khadrawy, Dina Sabry, Rasha E. Abo-ELeneen, Osama M. Ahmed
Alzheimer’s disease (AD) is the most frequent cause of age-related neurodegeneration and ensuing cognitive impairment. Progressive deposition of extracellular amyloid beta (Aβ) aggregates (plaques) and intracellular hyperphosphorylated Tau protein (p-Tau) are the core pathological markers of AD but may precede clinical symptoms by many years, presenting a therapeutic window of opportunity. Females
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Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-κB Pathways Stem Cells Int. (IF 4.3) Pub Date : 2023-5-24 Yiqiang Hu, Yuan Xiong, Kangkang Zha, Rangyang Tao, Lang Chen, Hang Xue, Chenchen Yan, Ze Lin, Yori Endo, Faqi Cao, Wu Zhou, Guohui Liu
Bone mesenchymal stem cells (BMSCs) play an important role in maintaining the dynamic balance of bone metabolism. Recent studies have reported that a decrease in the osteogenic function of MSCs is strongly associated with osteoporosis. Melatonin is a neuroendocrine hormone produced in the pineal gland and is essential in the physiological regulation. This study is aimed at exploring the effect of melatonin
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Fabrication of ECM Mimicking Bioactive Scaffold: A Regenerative Approach for MSC Mediated Applications Stem Cells Int. (IF 4.3) Pub Date : 2023-5-20 Pavani Koka, Yamini Chandramohan, Elumalai Perumal, Avinash Kavarthapu, Anuradha Dhanasekaran, Anusha Chandran, Krishnamoorthy Gunasekaran
Biomaterials are feasible resources that aids to replace damaged structures in our bodies. The most biologically active flora is Aloe vera which has many bioactive compounds that are anti-inflammatory, antimicrobial, and have ECM mimicking protein content which helps in the healing of wounds and also acts as an ECM factor for stem cell homing and differentiation. The Aloe vera containing 10 of gelatin
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Spermidine Retarded the Senescence of Multipotent Mesenchymal Stromal Cells In Vitro and In Vivo through SIRT3-Mediated Antioxidation Stem Cells Int. (IF 4.3) Pub Date : 2023-5-17 Hua Huang, Wen Zhang, Junjie Su, Bisheng Zhou, Qingjiang Han
Multipotent mesenchymal stromal cells (MSCs) expand in vitro and undergo replicative senescence, thereby restricting their clinical utilization. Thus, an effective strategy is required to impede MSC senescence. Since spermidine (SPD) supplementation can prolong the lifespan of yeast by inhibiting oxidative stress, spermidine is a potential option for delaying MSC senescence. In this study, to test
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Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins Stem Cells Int. (IF 4.3) Pub Date : 2023-5-15 Alessia Parascandolo, Michele Francesco Di Tolla, Domenico Liguoro, Manuela Lecce, Saverio Misso, Fabiana Micieli, Maria Rosaria Ambrosio, Serena Cabaro, Francesco Beguinot, Alessandra Pelagalli, Vittoria D’Esposito, Pietro Formisano
Platelet products are commonly used in regenerative medicine due to their effects on the acceleration and promotion of wound healing, reduction of bleeding, synthesis of new connective tissue, and revascularization. Furthermore, a novel approach for the treatment of damaged tissues, following trauma or other pathological damages, is represented by the use of mesenchymal stem cells (MSCs). In dogs,
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Pancancer Analysis Revealed the Value of RAC2 in Immunotherapy and Cancer Stem Cell Stem Cells Int. (IF 4.3) Pub Date : 2023-5-12 Ranran Liu, Tianyu Li, Guohong Zhang, Yejuan Jia, Jingxuan Liu, Lijia Pan, Yunfeng Li, Chunsheng Jia
Objective. To investigate the oncogenic effect and clinical significance of RAC2 in pancarcinoma from the perspective of tumor immunity and cancer stem cell. Methods. After in-depth mining of TCGA, GEO, UCSC, and other databases, basic information of the RAC2 gene and its expression in tumor tissues as well as the relationship between RAC2 and tumor were analyzed based on survival, mutation, immune
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The Effects of Programmed Cell Death of Mesenchymal Stem Cells on the Development of Liver Fibrosis Stem Cells Int. (IF 4.3) Pub Date : 2023-5-11 Hong-wei Wu, He-dan Chen, Ya-hong Chen, Xin-li Mao, Yu-yi Feng, Shao-wei Li, Xian-bin Zhou
Mesenchymal stem cells have shown noticeable potential for unlimited self-renewal. They can differentiate into specific somatic cells, integrate into target tissues via cell-cell contact, paracrine effects, exosomes, and other processes and then regulate the target cells and tissues. Studies have demonstrated that transplantation of MSCs could decrease the expression and concentration of collagen in
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Vascularization of a Bone Organoid Using Dental Pulp Stem Cells Stem Cells Int. (IF 4.3) Pub Date : 2023-5-9 Aonan Li, Jun-Ichi Sasaki, Gabriela L. Abe, Chihiro Katata, Hirohiko Sakai, Satoshi Imazato
Bone organoids offer a novel path for the reconstruction and repair of bone defects. We previously fabricated scaffold-free bone organoids using cell constructs comprising only bone marrow-derived mesenchymal stem cells (BMSCs). However, the cells in the millimetre-scale constructs were likely to undergo necrosis because of difficult oxygen diffusion and nutrient delivery. Dental pulp stem cells (DPSCs)
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MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T Cells Stem Cells Int. (IF 4.3) Pub Date : 2023-5-9 Min Dong, Guozhen Zhang, Jie Meng, Biou Liu, Duanfeng Jiang, Feng Liu
Purpose. Dendritic cells (DC) are specialized antigen-presenting cells, and cytokine-induced killer (CIK) cells have a specific killing activity to a variety of tumors. However, the underlining mechanism and function of DC-CIK cells in acute myeloid leukemia (AML) remain largely elusive. Methods. Gene expression profiles of leukemia patients were obtained from TCGA, DC cell components were evaluated