-
Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L- xylulose biotransformation from xylitol Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-22 Mesfin Angaw Tesfay, Xin Wen, Yujie Liu, Huibin Lin, Linxu Chen, Jianqiang Lin, Jianqun Lin
L-Xylulose is a rare ketopentose which inhibits α-glucosidase and is an indicator of hepatitis or liver cirrhosis. This pentose is also a precursor of other rare sugars such as L-xylose, L-ribose or L-lyxose. Recombinant E. coli expressing xylitol-4-dehydrogenase gene of Pantoea ananatis was constructed. A cost-effective culture media were used for L-xylulose production using the recombinant E. coli
-
Model-assisted DoE software: optimization of growth and biocatalysis in Saccharomyces cerevisiae bioprocesses Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-20 André Moser, Kim B. Kuchemüller, Sahar Deppe, Tanja Hernández Rodríguez, Björn Frahm, Ralf Pörtner, Volker C. Hass, Johannes Möller
Bioprocess development and optimization are still cost- and time-intensive due to the enormous number of experiments involved. In this study, the recently introduced model-assisted Design of Experiments (mDoE) concept (Möller et al. in Bioproc Biosyst Eng 42(5):867, https://doi.org/10.1007/s00449-019-02089-7, 2019) was extended and implemented into a software (“mDoE-toolbox”) to significantly reduce
-
Formation of electroactive biofilms derived by nanostructured anodes surfaces Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-09 Rehab H. Mahmoud, Farag A. Samhan, Mohamed K. Ibrahim, Gamila H. Ali, Rabeay Y. A. Hassan
-
Immobilization of Amano lipase AK from Pseudomonas fluorescens on different types of chitosan-containing supports: use in the kinetic resolution of rac -indanol Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Thiago de Sousa Fonseca, Ulisses Marcondes Freire de Oliveira, Maria da Conceição Ferreira de Oliveira, Telma Leda Gomes de Lemos, Marcos Reinaldo da Silva, Nathalia Saraiva Rios, Luciana Rocha Barros Gonçalves, Marcos Carlos de Mattos
Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY) of 81, 81 and 83%, respectively. Best activity results were achieved using chitosan
-
High level production of itaconic acid at low pH by Ustilago maydi s with fed-batch fermentation Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Hatice Taşpınar Demir, Emine Bezirci, Johanna Becker, Hamed Hosseinpour Tehrani, Emrah Nikerel, Nick Wierck, Mustafa Türker
The metabolically engineered plant pathogen Ustilago maydis MB215 Δcyp3 Petefria1 has been cultivated to produce more than 80 g/L itaconate in 16 L scale pH and temperature controlled fermentation, in fed-batch mode with two successive feedings. The effect of pH as well as successive rounds of feeding has been quantified via elemental balances. Volumetric itaconic acid productivity gradually decreased
-
Cross-feeding and wheat straw extractives enhance growth of Clostridium thermocellum -containing co-cultures for consolidated bioprocessing Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Alan G. Froese, Richard Sparling
Co-cultures consisting of three thermophilic and lignocellulolytic bacteria, namely Clostridium thermocellum, C. stercorarium, and Thermoanaerobacter thermohydrosulfuricus, degrade lignocellulosic material in a synergistic manner. When cultured in a defined minimal medium two of the members appeared to be auxotrophic and unable to grow, but the growth of all species was observed in all co-culture combinations
-
Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Luciana Luft, Tássia C. Confortin, Izelmar Todero, José R. Chaves Neto, Marcus V. Tres, Giovani L. Zabot, Marcio A. Mazutti
Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass. These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield. A central composite rotational design was performed for each extraction method
-
Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Patrícia M. B. Nunes, Jully L. Fraga, Rafael B. Ratier, Maria Helena M. Rocha-Leão, Ana I. S. Brígida, Patrick Fickers, Priscilla F. F. Amaral
The lipolytic yeast Yarrowia lipolytica produces cell-wall-associated lipases, namely Lip7p and Lip8p, that could have interesting properties as catalyst either in free (released lipase fraction—RLF) or cell-associated (cell-bound lipase fraction—CBLF) forms. Herein, a mixture of waste soybean frying oil, yeast extract and bactopeptone was found to favor the enzyme production. Best parameters for lipase
-
Cell-surface engineering of yeasts for whole-cell biocatalysts Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-03 Mengqi Ye, Yuqi Ye, Zongjun Du, Guanjun Chen
Due to the unique advantages comparing with traditional free enzymes and chemical catalysis, whole-cell biocatalysts have been widely used to catalyze reactions effectively, simply and environment friendly. Cell-surface display technology provides a novel and effective approach for improved whole-cell biocatalysts expressing heterologous enzymes on the cell surface. They can overcome the substrate
-
Simultaneous nitrification and denitrification in moving bed bioreactor and other biological systems Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-02 Roumi Bhattacharya, Debabrata Mazumder
Moving bed bioreactor (MBBR), used for treatment of municipal and industrial wastewater, is a completely mixed attached growth type system that involves microorganisms which grow as biofilm on the surface of the suspended carriers within the reactor. If the biofilm is thick enough, dissolved oxygen in the reactor would not diffuse into deeper strata and thus anoxic/anaerobic condition develops in those
-
Feeding strategies to optimize vanillin production by Amycolatopsis sp. ATCC 39116 Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-02 Rita Valério, Ana R. S. Bernardino, Cristiana A. V. Torres, Carla Brazinha, Maria L. Tavares, João G. Crespo, Maria A. M. Reis
The growing consumer demand for natural products led to an increasing interest in vanillin production by biotechnological routes. In this work, the biotechnological vanillin production by Amycolatopsis sp. ATCC 39116 is studied using ferulic acid as precursor, aiming to achieve maximized vanillin productivities. During biotech-vanillin production, the effects of glucose, vanillin and ferulic acid concentrations
-
Development of a purification process via crystallization of xylitol produced for bioprocess using a hemicellulosic hydrolysate from the cashew apple bagasse as feedstock Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2021-01-02 José Edvan Marques Júnior, Maria Valderez Ponte Rocha
Xylitol was biotechnologically produced by Kluyveromyces marxianus ATCC36907 using the hemicellulosic hydrolysate of the cashew apple bagasse (CABHH). Sequentially, the present study investigated the recovery and purification of xylitol evaluating different antisolvents [ethanol, isopropanol and the ionic liquid 2-hydroxyl-ethylammonium acetate (2-HEAA)], their proportion in the medium (10–90% v/v)
-
Dynamic parameter estimation and prediction over consecutive scales, based on moving horizon estimation: applied to an industrial cell culture seed train Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-12-29 Tanja Hernández Rodríguez, Christoph Posch, Ralf Pörtner, Björn Frahm
Bioprocess modeling has become a useful tool for prediction of the process future with the aim to deduce operating decisions (e.g. transfer or feeds). Due to variabilities, which often occur between and within batches, updating (re-estimation) of model parameters is required at certain time intervals (dynamic parameter estimation) to obtain reliable predictions. This can be challenging in the presence
-
Preparation and synthetic dye decolorization ability of magnetic cross-linked enzyme aggregates of laccase from Bacillus amyloliquefaciens Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-27 Hongrao Wang, Shuran Han, Jiayi Wang, Shuyu Yu, Xiaoyan Li, Lei Lu
Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during the synthesis of M-CLEAs. With ammonium sulfate as precipitant, maximum activity was
-
Influence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-25 Marieke E. Klijn, Jürgen Hubbuch
The protein cloud-point temperature (TCloud) is a known representative of protein–protein interaction strength and provides valuable information during the development and characterization of protein-based products, such as biopharmaceutics. A high-throughput low volume TCloud detection method was introduced in preceding work, where it was concluded that the extracted value is an apparent TCloud (TCloud
-
The application portfolio empowerment method and ELECTRE-I for optimising the control of ammonia release during the aerobic fermentation process Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-24 Yuanyuan Wu, Haoming Li, Shuai Zou, Guomin Wang, Haifu Jiang, Fuchuan Huang
Aerobic fermentation composting can transform solid organic waste into biological organic fertiliser, while reducing resource wastage and ecological damage. However, in the composting process, a serious loss of nitrogen occurs, primarily in the form of the release of ammonia gas. The release of ammonia gas not only pollutes the environment, but also diminishes the presence of nutrient elements, resulting
-
The impact of clearance on mixing time for interface-added substrate Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-23 Poorya Mirzavand Borujeni, Ehsan Ebrahimpoor, Navid Mostoufi
This study was carried out to find the optimum clearance (impeller to bottom distance) for Rushton and pitch-blade turbine impellers in a stirred tank bioreactor for improved substrate mixing time added at interface, taking advantage of computational fluid dynamics. In this regard, the time needed for a thin layer of liquid, resembling substrate-rich or poor part, getting homogenously dispersed within
-
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-22 Caroline Lopes Perez, Fernanda Perpétua Casciatori, João Cláudio Thoméo
Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale
-
Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-20 Xiumei Wang, Xiaoxu Zhao, Xiaoli Qin, Zexin Zhao, Bo Yang, Yonghua Wang
This study reports the properties of immobilized MAS1-H108A lipase from marine Streptomyces sp. strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively.
-
Bacterial cellulose as an oleaginous yeast cell carrier for soybean oil refinery effluent treatment and pyrolysis oil production Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-19 Nan Qiao, Xue Fan, Shuang Hu, Xiuzhen Zhang, Ling Wang, Yundi Du, Lei Wang, Xiaojun Zhang, Dayu Yu
Bacterial cellulose produced from soybean oil refinery effluent is a good immobilization carrier because of the large pores in its fiber network, its high water-holding capacity, and its good biocompatibility. In this study, it was applied to immobilization of oleaginous yeasts for treating soybean oil refinery effluent. The immobilization percentage reached 50%, and the removal of chemical oxygen
-
Effect of baffle structure on flow field characteristics of orbitally shaken bioreactor Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-16 Zhiming Lu, Chengtuo Li, Liangqi Fei, Hongliang Zhang, Yuhui Pan
Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were simulated, and the turbulence, dissolved oxygen and shear strain rate of the bioreactor were analyzed
-
High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-16 Mianhui Chen, Jingjing Wang, Lin Lin, Wei Wei, Yaling Shen, Dongzhi Wei
-
Effect of the tetracycline antibiotics on performance and microbial community of microbial fuel cell Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-12 Şeyho Topcu, Ergin Taşkan
-
Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-10 Chadathorn Inyai, Gorawit Yusakul, Jukrapun Komaikul, Tharita Kitisripanya, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Waraporn Putalun
Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be promising anti-COVID-19 agents. However, conventional cultivation of MA does not meet the demand for its stilbenes, and root quality usually varies between crops. This
-
Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP–Abs and Prussian blue nanocomposite for organophosphorus pesticide detection Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-08 Haowei Dong, Qingxue Zhao, Jiansen Li, Yaodong Xiang, Huimin Liu, Yemin Guo, Qingqing Yang, Xia Sun
Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs
-
Two-step enzymatic synthesis of α-linolenic acid-enriched diacylglycerols with high purities from silkworm pupae oil Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-07 Xuan Liu, Wei Shi, Long Xu, Bo Yang, Sentai Liao, Dongming Lan, Weifei Wang, Yonghua Wang
In this study, α-linolenic acid-enriched diacylglycerols (ALA-DAGs) were prepared via a two-step enzymatic way by combi-lipase using silkworm pupae oils as substrates. Firstly, several factors including temperature, mass ratio of water to oil, pH and enzyme loading were optimized for the hydrolysis of silkworm pupae oil. The maximum fatty acid content (96.51%) was obtained under the conditions: temperature
-
Transcriptome–metabolome analysis of fatty acid of Bamei pork and Gansu Black pork in China Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-07 Bin Xi, Jin Luo, Ya-qin Gao, Xiao-ling Yang, Tian-fen Guo, Wei-hong Li, Tian-qing Du
To study the difference in transcriptome level of fatty acid metabolism pathway in Bamei pork and the difference of pork quality caused by the difference. In this study, Bamei pigs breeding in Huzhu farm of QingHai province were selected as the test object, compared with Gansu Black pigs. Four indexes of nutmeg acid (DX1), palmitic acid (DX2), stearic acid (DX3) and linoleic acid (DX4) were set. The
-
Status of the application of exogenous enzyme technology for the development of natural plant resources Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-04 Bin Yuan, Shiyu Zhou, Changwei Liu, Sheng Zhang, Jiayin Li, Ailing Liu
Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich source of enzymes. Amylase, cellulase, protease, pectinase, glycosidase, tannase, and polyphenol
-
CeO 2 foam-like nanostructure: biosynthesis and their efficient removal of hazardous dye Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-11-02 Aliakbar Alinaghi Langari, Simin Soltaninezhad, Niloofar Zafarnia, Mohammadreza Heidari, Rajender S. Varma, Zahra Ebrahimi, Sara Azhdari, Fariba Borhani, Mehrdad Khatami
In this study, CeO2 (cerium oxide) nanoparticles were synthesized using Pinus halepensis pollen and were characterized by field emission scanning electron microscopy (FESEM), powder X-ray diffraction (PXRD) and Raman spectroscopy. The results showed that the ensuing CeO2 nanostructures, ranging in size from 5 to 25 nm, had high porosity. Synthesized CeO2 showed the effective catalytic activity towards
-
Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite-β for enzyme recycling applications Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-16 Surya R. Jampana, Linjing Jia, Bandaru V. Ramarao, Deepak Kumar
-
Expression of porcine interferon-α and its bioactivity analysis in vitro and in vivo Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-13 Pengtao Wang, Lu Xia, Xiuli Liang, Fangfang Han, Haojie Ren, Yixuan Zhang, Zhanyong Wei
Interferon α (IFN-α) plays a crucial role in the host’s immune response. In this study, the amino acid sequence of porcine interferon α (PoIFN-α) was analyzed. Seven substitutions, S38F, H40Q, F43L, N78D, Y86C, S151A, and R156T, were mutated and obtained by aligning the sequences of PoIFN-α subtypes. The PoIFN-α mutants were designed, expressed, and purified in E. coli. The antiviral activities of
-
Amino modified magnetic halloysite nanotube supporting chloroperoxidase immobilization: enhanced stability, reusability, and efficient degradation of pesticide residue in wastewater Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-12 Xuefang Zhu, Xueting Fan, Yuting Wang, Quanguo Zhai, Mancheng Hu, Shuni Li, Yucheng Jiang
-
Biogenic preparation and characterization of Pyropia yezoensis silver nanoparticles ( P.y AgNPs) and their antibacterial activity against Pseudomonas aeruginosa Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-11 Selvakumari Ulagesan, Taek-Jeong Nam, Youn-Hee Choi
Marine algae play key roles in several medical, pharmaceutical, agricultural, and aquacultural applications. Furthermore, biosynthesized nanomaterials are becoming an alternative to conventional antibiotics in cost-effective, biocompatible, and non-toxic treatments for bacterial infections. This study features biogenic synthesis of silver nanoparticles using an aqueous extract of the marine red algae
-
Biological assisted organic sulfur removal from low rank indigenous coal using airlift bioreactor Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-09 Abdul Sattar Jatoi, Shaheen Aziz, Suhail Ahmed Soomro
Combustion of coal create many harmful gases which effect on human health as well as on environment. The sulfur in coal limits its own use, and bio-desulfurization (BDS) shows enormous development potential and the prospects for the application of coal desulfurization. Present study highlights the bioprocess strategies for reduction of sulfur content from coal before combustion. The bioprocess involved
-
Environmental pollution reducing strategy for scouring of undegummed sisal fibers using xylanase and pectinase enzymes Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-08 Avtar Singh, Libin Mathew Varghese, Bindu Battan, Arun Kumar Patra, Rishi Pal Mandhan, Ritu Mahajan
This study was undertaken to investigate the potential of bioscouring in the processing of undegummed sisal fibers, using xylano-pectinolytic enzymes. Optimum bioscouring was obtained at pH 8.5 and 50 mM buffer molarity, using xylanase (10 IU) and pectinase (8 IU), with a material to liquor proportion of 1:25 (g:ml), EDTA (2 mM) and Tween 80 (0.5%), at 50 °C temperature with agitation rate of 55 rpm
-
Limited oxygen conditions as an approach to scale-up and improve d and l -lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-10-07 Estefanía Sierra-Ibarra, Laura J. Leal-Reyes, Gerardo Huerta-Beristain, Ana L. Hernández-Orihuela, Guillermo Gosset, Agustino Martínez-Antonio, Alfredo Martinez
The effectiveness of micro-aeration on lactate (LA) production by metabolically engineered Escherichia coli was evaluated in 1 L bioreactors containing mineral media and glucose (70 g/L). Volumetric oxygen transfer coefficients (kLa) between 12.6 and 28.7 h−1 increased the specific growth rate (µ) and volumetric productivity (QLA) by 300 and 400%, respectively, without a significant decrease in lactate
-
Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-27 Ying Wang,Yu-Lan Zou,Hu Chen,Yong-Kang Lv
A new aerobic denitrifying bacterium ZYL was isolated from domestic wastewater sludge and identified as Acinetobacter haemolyticus (similarity 99%) by the 16S rDNA sequencing analysis. The strain could use nitrate, nitrite and ammonium as the sole N-source for growth with a final product of N2, demonstrating its good abilities for aerobic denitrification and heterotrophic nitrification. Single-factor
-
Biohydrogen production in an AFBR using sugarcane molasses. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-26 Taciana Carneiro Chaves,Georgia Nayane Silva Belo Gois,Fernanda Santana Peiter,Daniele Vital Vich,Eduardo Lucena Cavalcante de Amorim
This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L-1. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic retention times of 12, 6, 4 and 3 h. The maximum hydrogen production rate (1.44 L-H2 h-1
-
Enhancing electrochemical sensing for catechol by biomimetic oxidase covalently functionalized graphene oxide. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-23 Xia Jiaojiao,Wang Pengyun,Zou Bin,Adesanya Idowu Onyinye
Catechol level is an important indicator for evaluating the quality of tea. Therefore, the exploration of a simple and efficient quantitative detection method for catechol has an important significance. In this study, functionalized graphene oxide was synthesized by chemically modifying the surface of graphene oxide. The prepared carrier was covalently combined with biomimetic oxidase iron porphyrin
-
Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-21 Punniyakotti Elumalai,Mohamad S AlSalhi,Sanjeet Mehariya,Obulisamy Parthiba Karthikeyan,Sandhanasamy Devanesan,Punniyakotti Parthipan,Aruliah Rajasekar
This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, including Marinobacter
-
Directed evolution of Aspergillus oryzae lipase for the efficient resolution of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-21 Mengjie Zhang,Qi Li,Xing Lan,Xiaojun Li,Yinjun Zhang,Zhao Wang,Jianyong Zheng
Aspergillus oryzae lipase (AOL) is a potential biocatalyst for industrial application. In this study, a mutant lipase AOL-3F38N/V230R was screened through two rounds of directed evolution, resulting in a fourfold increase in lipase activity, and threefold in catalytic efficiency (kcat/Km), while maintaining its excellent stereoselectivity. AOL-3F38N/V230R enzyme activity was maximum at pH 7.5 and also
-
Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-21 Alireza Habibi,Salar Karami,Kambiz Varmira,Malihe Hadadi
-
Enhancement of schizophyllan production in Schizophyllum commune using microparticles in medium. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-21 Vahid Alizadeh,Seyed Abbas Shojaosadati,Seyed Morteza Zamir
Schizophyllum commune is a wood-rotting filamentous fungus that secrets a homopolysaccharide called as schizophyllan. Schizophyllan has several applications such as enhanced oil recovery, pharmaceutical materials and an anti-cancer drug carrier. Biomass growth and schizophyllan production increase the viscosity of the cultivation medium, thus resulting in mass transfer limitation for the substrate
-
Deconstruction of banana peel for carbohydrate fractionation. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-18 Maria Angélica F Pereira,Carla Roana M Monteiro,Gabriela N Pereira,Sidnei E Bordignon Júnior,Eduardo Zanella,Patrícia F Ávila,Boris U Stambuk,Rosana Goldbeck,Débora de Oliveira,Patrícia Poletto
The deconstruction of banana peel for carbohydrate recovery was performed by sequential treatment (acid, alkaline, and enzymatic). The pretreatment with citric acid promoted the extraction of pectin, resulting in a yield of 8%. In addition, xylose and XOS, 348.5 and 17.3 mg/g xylan, respectively, were also quantified in acidic liquor as a result of partial depolymerization of hemicellulose. The spent
-
Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-17 Ping Zhu,Yijing Zhan,Cheng Wang,Xiaoliu Liu,Liming Liu,Hong Xu
Cell growth and product formation are two critical processes in polysaccharide welan biosynthesis, but the conflict between them is often encountered. In this study, a temperature-dependent strategy was designed for two-stage welan production through overexpressing heat shock proteins in Sphingomonas sp. The first stage was cell growth phase with higher TCA cycle activity at 42 °C; the second stage
-
A kinetic study on carboxylic acids production using bovine slaughterhouse wastewater: a promising substrate for resource recovery in biotechnological processes. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-11 Naassom Wagner Sales Morais,Milena Maciel Holanda Coelho,Tasso Jorge Tavares Ferreira,Erlon Lopes Pereira,Renato Carrhá Leitão,André Bezerra Dos Santos
Carboxylic acids (CA) are considered high added-value compounds, and their production from wastes has gained economic and environmental notoriety. However, the CA production and kinetic modeling using some agro-industrial wastewaters, such as bovine slaughterhouse wastewater (SHW), are not well reported in the literature. Therefore, the objective of this work was to evaluate the CA production potential
-
Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-07 Mengxing Li,Mark R Wilkins
Polyhydroxybutyrate (PHB) is a biodegradable bioplastic that is comparable with many petroleum-based plastics in terms of mechanical properties and is highly biocompatible. Lignocellulosic biomass conversion into PHB can increase profit and add sustainability. Glucose, xylose and arabinose are the main monomer sugars derived from upstream lignocellulosic biomass processing. The sugar mixture ratios
-
Evaluation of the production of alginate-like exopolysaccharides (ALE) and tryptophan in aerobic granular sludge systems. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-05 Silvio Luiz de Sousa Rollemberg,Amanda Ferreira Dos Santos,Tasso Jorge Tavares Ferreira,Paulo Igor Milen Firmino,André Bezerra Dos Santos
The engineering and microbiological aspects involved in the production of alginate-like exopolysaccharides (ALE) and tryptophan (TRY) in aerobic granular sludge systems were evaluated. The inclusion of short anoxic phase (A/O/A cycle–anaerobic, oxic, and anoxic phase) and the control of sludge retention time (SRT ≈ 10 days) proved to be an important strategy to increase the content of these bioproducts
-
A selected bacterial strain for the self-healing process in cementitious specimens without cell immobilization steps. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-05 Romario P Santos,Tatiane M Ramos,Brendo M Borges,Luciana M Hollanda,Álvaro S Lima,Cleide M F Soares,Ranyere L Souza
The use of microorganisms capable of mediating the bioprecipitation process can be an important application in the self-healing processes of cement specimens. Thus, the present study identified and evaluated five Bacillus strains for potential application in the protocol of self-healing via bioprecipitation. Cell growth, enzyme production, and kinetic parameters conditions were evaluated during the
-
Efficacy of red light for enhanced cell disruption and fluorescence intensity of phycocyanin. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-04 Sivaprakasam Sivasankari,Mani Vinoth,David Ravindran,Kathirvelu Baskar,Abdulaziz A Alqarawi,Elsayed Fathi Abd Allah
In this study, red LED and urea used as light and nitrogen sources, respectively, for the cultivation of Spirulina to enhance the fluorescence property and purity of phycocyanin. Besides, there is a high concentration of phycocyanin leached out from red light (RL) grown cells than white light (WL) without cell disruption. This type of cultivation reduces the complexity of extraction methods and cost
-
Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05T extracellular proteases. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-04 Nelda L Olivera,Cynthia Sequeiros,Martín Iglesias,Marina Nievas
-
Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-04 Xiao Guo,Ailing Guo,Erhu Li
Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside
-
Statistical approach to evaluate effect of temperature and moisture content on the production of antioxidant naphtho-gamma-pyrones and hydroxycinnamic acids by Aspergillus tubingensis in solid-state fermentation. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-03 Quentin Carboué,Catherine Rébufa,Rayhane Hamrouni,Sevastianos Roussos,Isabelle Bombarda
Non-ochratoxigenic Aspergillus tubingensis G131 is a filamentous fungus that can produce naphtho-gamma-pyrones (NγPs), polyketide pigments that exhibit interesting antioxidant properties. This study aims to investigate the effect of two critical parameters, temperature and moisture content on the fungus grown in solid-state fermentation using agricultural by-products (vine shoots and wheat bran) as
-
Investigating the potential of locally sourced wastewater as a feedstock of microbial desalination cell (MDC) for bioenergy production. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-09-01 Rabia Liaquat,Tariq Mehmood,Asif Hussain Khoja,Naseem Iqbal,Haider Ejaz,Sadia Mumtaz
Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells (MDCs) represent a sustainable option that has added benefit of solving the ever-increasing
-
The effect of Np-magnetite on the granulation process of an SBR reactor used for domestic wastewater treatment. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-08-29 Dayane Gonzaga Domingos,Nelson Libardi,Rosana Oliveira Henriques,Jéssica Antunes Xavier,Rejane Helena Ribeiro da Costa
This study investigated the effect of magnetite nanoparticles (Np-magnetite) added to a pilot-scale sequencing batch reactor (SBR) treating domestic wastewater, to improve aerobic granular sludge (AGS) formation and the effects of granule disintegration. Np-magnetite additions (75 mg L-1) were made during the start-up of the reactor and repeated after 100 and 170 days, when granule disintegration was
-
Bioprocessing and integration of a high flux screening systematic platform based on isothermal amplification for the detection on 8 common pathogens. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-08-29 Huamin Zhong,Hongwei Deng,Ming Li,Huahong Zhong
During a large variety of common pathogens, E. coli, P. aeruginosa, MRSA, MRCNS, V. parahaemolyticus, L. monocytogenes and Salmonella are the leading pathogens responsible for large number of human infections and diseases. In this study, a high flux screening based on nucleic acid isothermal amplification technique has been developed. For the 8 common pathogens, species-specific targets had been selected
-
Bioprocess strategies for enhancing the outdoor production of Nannochloropsis gaditana: an evaluation of the effects of pH on culture performance in tubular photobioreactors. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-06-25 L Moraes,G M Rosa,I M Cara,L O Santos,M G Morais,E Molina Grima,J A V Costa,F G Acién Fernández
A priority of the industrial applications of microalgae is the reduction of production costs while maximizing algae biomass productivity. The purpose of this study was to carry out a comprehensive evaluation of the effects of pH control on the production of Nannochloropsis gaditana in tubular photobioreactors under external conditions while considering the environmental, biological, and operational
-
Reaction coupling separation for isosteviol production from stevioside catalyzed by acidic ion-exchange resin. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-08-28 Xueyi Hu,Zhuoyu Zhou,Zongying Zhang,Xiaoxia Wang,Xiaochen Sui,Junming Chen,Yongmei Xia,Jue Zhang,Jianguo Lin
-
Assessment and evaluation of cellulase production using ragi (Eleusine coracana) husk as a substrate from thermo-acidophilic Aspergillus fumigatus JCM 10253. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-08-26 Paramjeet Saroj,Manasa P,Korrapati Narasimhulu
The cellulase production by filamentous fungi Aspergillus fumigatus JCM 10253 was carried out using agro-industrial waste ragi husk as a substrate in the microbial fermentation. The effect of the process parameters such as temperature, substrate concentration, pH, and incubation process time and their interdependence was studied using response surface methodology. The optimum cellulase activities were
-
Mass transfer in aerated culture media combining mixed electrolytes and glucose. Bioprocess Biosyst. Eng. (IF 2.419) Pub Date : 2020-08-25 Oscar R Góngora-García,Gloria Aca-Aca,Sergio A Baz-Rodríguez
The combined effects of mixed electrolyte species and glucose on oxygen transfer were studied in a bubble column with aqueous solutions. Of particular interest was the presence of electrolytes containing ions which are prone to present solute–solute interactions or to crystallize. Without and at low concentration of glucose (≤ 5 g/L), the increasing concentration of electrolytes (nominal ionic strength: