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Scaffold-free BMSC sheets enhance bone formation in a weight-bearing rat critical bone defect model. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-29 Kazuaki Mito,Jordan Lachnish,Wei Le,Calvin Chan,Yun-Liang Chang,Jeff Yao
Researchers have been exploring alternative methods for bone tissue engineering, as current management of large bone defects may be a significant challenge for both patient and surgeon with conventional surgical treatments leading to high complication and revision rates. Recent studies have shown mesenchymal stem cell sheets may enhance bone regeneration in different animal models. We investigated
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Smart Design for Hybrid Bioprinting of Scalable and Viable Tissue Constructs. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-29 Niji Nandakumar,Subramania Iyyer,Thadi Mohan,Shantikumar V Nair,Binulal N Sathy
Hybrid bioprinting uses sequential printing of melt-extruded biodegradable thermoplastic polymer and cell-encapsulated bioink in a predesigned manner using high- and low-temperature print heads for the fabrication of robust three-dimensional (3D) biological constructs. However, the high-temperature print head and melt-extruded polymer cause irreversible thermal damage to the bioprinted cells, and it
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Biopatterning of 3D Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-17 Rabia Onbas,Ahu Arslan Yıldız
Patterning cells to create 3D cell culture models by magnetic manipulation is a promising technique, which is rapid, simple, and cost-effective. This study introduces a new biopatterning approach based on magnetic manipulation of cells with a bio-ink that consists alginate, cells, and magnetic nanoparticles (MNPs). Plackett-Burman and Box-Behnken experimental design models were used to optimize bio-ink
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4D Printed Nerve Conduit with In Situ Neurogenic Guidance for Nerve Regeneration. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-15 Haitao Cui,Wei Zhu,Shida Miao,Kausik Sarkar,Lijie Grace Zhang
Nerve repair poses a significant challenge in the field of tissue regeneration. As a bioengineered therapeutic method, nerve conduits have been developed to address damaged nerve repair. However, despite their remarkable potential, it is still challenging to encompass complex physiologically microenvironmental cues (both biophysical and biochemical factors) to synergistically regulate stem cell differentiation
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Production of a Bioink Containing Decellularized Spinal Cord Tissue for 3D Bioprinting. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-15 Marcelo Garrido Dos Santos,Fernanda Stapenhorst França,João Pedro Prestes,Cristian Teixeira,Luiz Carlos Sommer,Laura Elena Sperling,Patricia Pranke
For the past few years, three-dimensional (3D) bioprinting has emerged as a promising approach in the field of regenerative medicine. This technique allows for the production of 3D scaffolds to support cell transplantation due to its ability to mimic the extracellular environment. One alternative to enhancing cell adhesion, survival, and proliferation is the use of decellularized extracellular matrix
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Effect of Cell Density of an MAA-Based Hydrogel Implant on Embedded Islet Function and Viability. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-14 Krystal M Ortaleza,Michael V Sefton
Subcutaneous delivery of islets in an methacrylic acid (MAA)-based hydrogel may offer a functional cure for type 1 diabetes (T1D). Here we show in mice that the hydrogel is able to provide sufficient vasculature to support islet function and viability, when islets are used at a low islet volume fraction (i.e., cell density). The Krogh Cylinder model was used to mathematically estimate the effect of
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Bacterial cellulose electrospun fiber mesh coated with chitin nanofibrils for eardrum repair. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-14 Bahareh Azimi,Atefeh Rasti,Alessandra Fusco,Teresa Macchi,Claudio Ricci,Mohammad Hosseinifard,Lorenzo Guazzelli,Giovanna Donnarumma,Roohollah Bagherzadeh,Masoud Latifi,Ipsita Roy,Serena Danti,Andrea Lazzeri
In this study, we develop a bio-based and bioactive nanofibrous patch based on bacterial cellulose (BC) and chitin nanofibrils (CNs) using an ionic liquid as a solvent for BC, aimed at tympanic membrane (TM) repair. Electrospun BC nanofiber meshes were produced via electrospinning, and surface-modified with CNs using electrospray. The rheology of the BC/ionic liquid system was investigated. The obtained
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Comparative study of immunodeficient rat strains in engraftment of hiPSC-derived airway epithelia. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-11 Yasuyuki Hayashi,Hiroe Ohnishi,Masayuki Kitano,Yo Kishimoto,Toshiaki Takezawa,Hideaki Okuyama,Masayoshi Yoshimatsu,Fumihiko Kuwata,Takeshi Tada,Keisuke Mizuno,Koichi Omori
The airway epithelia (AE) play a role in the clearance of foreign substances through ciliary motility and mucus secreted. We developed an artificial trachea that is made of collagen sponges and polypropylene mesh for the regeneration of the tracheal defect, and it was used for a clinical study. Then, a model in which the luminal surface of an artificial trachea covered with a human-induced pluripotent
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Deep Learning augmented osteoarthritis grading standardization. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-11 Lacksaya Nagarajan,Aadyant Khatri,Arnav Sudan,Raju Vaishya,Sourabh Ghosh
Manual grading of cartilage histology images for investigating the extent and severity of osteoarthritis (OA) involves critical examination of the cell characteristics, which makes this task tiresome, tedious and error-prone. This results in wide inter-observer variation, causing ambiguities in OA grade prediction. Such drawbacks of manual assessment can be overcome by implementing artificial intelligence
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Progenitor cell sources for 3D bioprinting of lymphatic vessels and potential clinical application. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-11 Inazio Arriola-Alvarez,Ibon Jaunarena,Ander Izeta,Hector Lafuente
The lymphatic system maintains tissue fluid homeostasis and is involved in transport of nutrients and immunosurveillance. It also plays a pivotal role in both pathological and regenerative processes. Lymphatic development in the embryo occurs by polarization and proliferation of lymphatic endothelial cells from the lymph sacs, i.e. lymphangiogenesis. Alternatively, lymphvasculogenesis further contributes
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Cell therapy by mesenchymal stromal cells versus myoblasts in a pig model of urinary incontinence. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-07 Jasmin Knoll,Bastian Amend,Niklas Harland,Simon Isser,Nicolas Beziere,Udo Kraushaar,Arnulf Stenzl,Wilhelm Aicher
The leading cause of stress urinary incontinence in women is the urethral sphincter muscle deficiency caused by mechanical stress during pregnancy and vaginal delivery. In men, prostate cancer surgery and injury of local nerves and muscles are associated with incontinence. Current treatment often fails to satisfy the patient's needs. Cell therapy may improve the situation. We therefore investigated
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LATS1/YAP1 Axis Controls Bone Regeneration on Distraction Osteogenesis by Activating Wnt/β-catenin. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-06 Kehan Li,Linan Liu,Hanghang Liu,Jiawei Xing,Pei Hu,Jian Song
The Hippo signaling pathway inhibits cell growth, and its components and functions are highly conserved in mammals. LATS1 is a core component of the Hippo signaling pathway associated with lymphatic invasion, astrogliosis, apoptosis, and autophagy. Nevertheless, the role of Hippo/LATS1 in osteogenesis remains unclear. Here, we used ribonucleic acid (RNA) lentiviruses to inhibit the expression of Lats1
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E2-loaded Microcapsules and BMSCs with Injectable Scaffolds for Endometrial Regeneration Application. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-06 Yuelin Wu,Shengyi Gu,Jonathan M Cobb,Griffin H Dunn,Taylor A Muth,Chloe J Simchick,Baoguo Li,Wujie Zhang,Xiaolin Hua
Bone marrow-derived mesenchymal stem cells (BMSCs) have been recognized as new candidates for the treatment of serious endometrial injuries. However, due to the local microenvironment of damaged endometrium, transplantation of BMSCs yielded disappointing results. In this study, Pectin-Pluronic® F-127 hydrogel as scaffolds were fabricated to provide three-dimensional architecture for the attachment
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Bioprinted Human Lung Cancer-Mimics for Tissue Diagnostics Applications. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-06 Mian Wang,Wanlu Li,Regina Sanchez Flores,Ling Cai,Carlos Ezio Garciamendez-Mijares,Scott Gill,David Snyder,Jasmine Millabas,David Chafin,Yu Shrike Zhang,Azita Djalilvand
Developing a reproducible and secure supply of customizable control tissues that standardizes for the cell type, tissue architecture, and preanalytics of interest for usage in applications including diagnostic, prognostic, and predictive assays, is critical for improving our patient care and welfare. The conventionally adopted control tissues directly obtained from patients are not ideal because they
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Impact of Human Recombinant Irisin on Tissue-Engineered Skeletal Muscle Structure and Function. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-06 Matthew H Nguyen,Christopher S Kennedy,Olga M Wroblewski,Eileen Su,Derek H Hwang,Lisa M Larkin
Tissue engineering of exogenous skeletal muscle units (SMUs) through isolation of muscle satellite cells from muscle biopsies is a potential treatment method for acute volumetric muscle loss (VML). A current issue with this treatment process is the limited capacity for muscle stem cell (satellite cell) expansion in cell culture, resulting in a decreased ability to obtain enough cells to fabricate SMUs
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Cellular Interactions in Cell Sheets Enhance Mesenchymal Stromal Cell Immunomodulatory Properties. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-01 Celia M Dunn,Sumako Kameishi,Tavie Parker,Yun-Kyoung Cho,Sun U Song,David W Grainger,Teruo Okano
Immune-related applications of mesenchymal stromal cells (MSCs) in cell therapy seek to exploit immunomodulatory paracrine signaling pathways to reduce inflammation. A key MSC therapeutic challenge is reducing patient outcome variabilities attributed to insufficient engraftment/retention of injected heterogenous MSCs. To address this, we propose directly transplantable human single-cell-derived clonal
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Bone marrow mobilization & local SDF-1α delivery enhances nascent supraspinatus muscle fiber growth. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-28 Leah E Anderson,Liane E Tellier,Keshav Rasesh Shah,Joseph J Pearson,Alexandra L Brimeyer,Edward A Botchwey,Johnna S Temenoff
Rotator cuff tear is a significant problem that leads to poor clinical outcomes due to muscle degeneration after injury. The objective of this study was to synergistically increase the number of pro-regenerative cells recruited to injured rotator cuff muscle through a novel dual treatment system, consisting of a bone marrow mobilizing agent (VPC01091), hypothesized to "push" pro-healing cells into
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Increasing Collagen to Bioink Drives Mesenchymal Stromal Cells-Chondrogenesis from Hyaline to Calcified Layers. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-30 Océane Messaoudi,Christel Henrionnet,Edwin-Joffrey Courtial,Laurent Grossin,Didier Mainard,Laurent Galois,Damien Loeuille,Christophe Marquette,Pierre Gillet,Astrid Pinzano
The bioextrusion of mesenchymal stromal cells (MSCs) directly seeded in a bioink enables the production of three-dimensional (3D) constructs, promoting their chondrogenic differentiation. Our study aimed to evaluate the effect of different type I collagen concentrations in the bioink on MSCs' chondrogenic differentiation. We printed 3D constructs using an alginate, gelatin, and fibrinogen-based bioink
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Nutrient Rescue of Early Maturing and Deteriorating Satellite Cell-Derived Engineered Muscle Tissue. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-19 Hironobu Takahashi,Kaho Ishiyama,Naoya Takeda,Tatsuya Shimizu
Engineered human muscle tissue is a promising tool for tissue models to better understand muscle physiology and diseases, since they can replicate many biomimetic structures and functions of skeletal muscle in vitro. We have developed a method to produce contractile muscle sheet tissues from human myoblasts, based on our cell sheet fabrication technique. This study reports that our tissue engineering
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Ultrasound-Targeted Microbubble Destruction Assisted Delivery of Platelet-Rich Plasma-Derived Exosomes Promoting Peripheral Nerve Regeneration. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-19 Dan Yi,Yongyi Zhang,Molin Li,Jian Chen,Xianghui Chen,Li Wang,Guanghui Xing,Siming Chen,Yaqiong Zhu,Yuexiang Wang
Peripheral nerve injury is prevalent and has a high disability rate in clinical settings. Current therapeutic methods have not achieved satisfactory efficacy, underscoring the need for novel approaches to nerve restoration that remains an active area of research in neuroscience and regenerative medicine. In this study, we isolated platelet-rich plasma-derived exosomes (PRP-exos) and found that they
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Decellularization of Massive Bone Allografts By Perfusion: A New Protocol for Tissue Engineering. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-12 Robin Evrard,Julie Manon,Louis Maistriaux,Chiara Rafferty,Lies Fieve,Ugo Heller,Olivier Cornu,Pierre Gianello,Thomas Schubert,Benoit Lengele
In terms of large bone defect reconstructions, massive bone allografts may sometimes be the only solution. However, they are still burdened with a high postoperative complication rate. Our hypothesis is that the immunogenicity of residual cells in the graft is involved in this issue. Decellularization by perfusion might therefore be the answer to process and create more biologically effective massive
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Deciphering the Cell-Specific Effect of Osteoblast-Macrophage Crosstalk in Periodontitis. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-06 Diego Jacho,Parto Babaniamansour,Raquel Osorio,Manuel Toledano,Agustín Rabino,Rafael Garcia-Mata,Eda Yildirim-Ayan
In periodontitis, the bone remodeling process is disrupted by the prevalent involvement of bacteria-induced proinflammatory macrophage cells and their interaction with osteoblast cells residing within the infected bone tissue. The complex interaction between the cells needs to be deciphered to understand the dominant player in tipping the balance from osteogenesis to osteoclastogenesis. Yet, only a
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Subcutaneously Transplanted Fresh Cartilage in Allogeneic and Xenogeneic Immunocompetent Mouse. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-10-01 Sakura Uto,Atsuhiko Hikita,Daisuke Mori,Tomoaki Sakamoto,Fumiko Yano,Shinsuke Ohba,Taku Saito,Tsuyoshi Takato,Kazuto Hoshi
Cartilage is considered to be immune privileged in general. Clinically, live cells are removed from subcutaneously transplanted allogeneic cartilage mainly for preservation and for infection control. However, because maintaining cartilage feature requires live chondrocyte, it would be beneficial to subcutaneously transplant cartilage with live chondrocyte even if it was allogeneic. We harvested femoral
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Assessing Efficacy of Afatinib towards Elastic Matrix Repair in Aortic Aneurysms. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-29 Simran Dayal,Anand Ramamurthi
Abdominal aortic aneurysm (AAA) is a critical, multifactorial cardiovascular disorder marked by localized dilatation of the abdominal aorta. A major challenge to countering the pathophysiology of AAAs lies in the naturally irreversible breakdown of elastic fibers in the aorta wall, which is linked to the poor elastogenicity of adult and diseased vascular smooth muscle cells (SMCs) and their impaired
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Macrophage Mechanoresponsiveness within 3D Tissue Matrix upon Mechanotherapy- Associated Strains. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-19 Parto Babaniamansour,Diego Jacho,Ashley Teow,Agustin Rabino,Rafael Garcia-Mata,Eda Yildirim-Ayan
Mechano-rehabilitation, also known as mechanotherapy, represents the forefront of noninvasive treatment for musculoskeletal tissue (MSK) disorders, encompassing conditions affecting tendons, cartilage, ligaments, and muscles. Recent emphasis has underscored the significance of macrophage presence in the healing of MSK tissues. However, a considerable gap still exists in comprehending how mechanical
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Development of a Self-Assembled Dermal Substitute from Human Fibroblasts Using Long-term Three-Dimensional Culture. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-13 Takashi Nakano,Hiroki Yamanaka,Michiharu Sakamoto,Itaru Tsuge,Yasuhiro Katayama,Susumu Saito,Jiro Ono,Tetsuji Yamaoka,Naoki Morimoto
Skin substitutes have emerged as an alternative to autografts for the treatment of skin defects. Among them, scaffold-based dermal substitutes have been extensively studied; however, they have certain limitations, such as delayed vascularization, limited elasticity, and the inability to achieve permanent engraftment. Self-assembled, cell-based dermal substitutes are a promising alternative that may
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A Novel Magnetic Manipulation Promotes Directional Growth of Periodontal Ligament Stem Cells. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-08 Weiguang Li,Weidong Tian,Yafei Wu,Shujuan Guo
Periodontium is the rally of soft and hard tissues, which will be devastated continuously by the compromise of periodontitis. Current periodontal therapeutic methods cannot effectively reconstruct periodontal ligament (PDL), which is oriented at an angle with tooth root and combined hard tissues to form cementum-PDL-alveolar bone complex. Hence, it is urgent to find new techniques for PDL reconstruction
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Tumor Necrosis Factor Alpha Preconditioned Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Enhance the Inhibition of Necroptosis of Acinar cells in Severe Acute Pancreatitis. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-05 Wangcheng Xie,Tingyi Luo,Zhilong Ma,Shaobo Xue,Xuyang Jia,Tingsong Yang,Zhenshun Song
Severe acute pancreatitis (SAP) is a common abdominal emergency with a high mortality rate and a lack of effective therapeutic options. Although mesenchymal stem cell (MSC) transplantation is a potential treatment for SAP, the mechanism remains unclear. It has been suggested that MSCs may act mainly through paracrine effects; therefore, we aimed to demonstrate the therapeutic efficacy of extracellular
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Cell-Derived Extracellular Matrix Fiber Scaffolds Improve Recovery from Volumetric Muscle Loss. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-11-21 Cassandra Reed,Tai Huynh,Jacob Schluns,Payton Phelps,Jamie Hestekin,Jeffrey C Wolchok
There are currently no surgical procedures that effectively address the treatment of volumetric muscle loss (VML) injuries that has motivated the development of implantable scaffolding. In this study, the effectiveness of an allogenic scaffold fabricated using fibers built from the extracellular matrix (ECM) collected from muscle fibroblast cells during growth in culture was explored using a hindlimb
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A Ready-to-Use Purified Exosome Product for Volumetric Muscle Loss and Functional Recovery. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-09-01 Lorenzo Mazzucchelli,Aida K Sarcon,Tony C T Huang,Jialun Li,Charlotte E Berry,Matthew T Houdek,Atta Behfar,Chunfeng Zhao,Steven L Moran
Large skeletal muscle defects owing to trauma or following tumor extirpation can result in substantial functional impairment. Purified exosomes are now available clinically and have been used for wound healing. The objective of this study was to evaluate the regenerative capacity of commercially available exosomes on an animal model of volumetric muscle loss (VML) and its potential translation to human
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Engineered Tissue Graft for Repair of Injured Infraspinatus Rotator Cuff Tendon. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-08-31 Christopher S Kennedy,Carla N Villacís Núñez,Andrea Poli,Emmanuel Vega-Soto,Ellen M Arruda,Lisa M Larkin
Rotator cuff tears constitute a vast majority of shoulder-related injuries, occurring in a wide population range and increasing in incidence with age. Current treatments for full thickness tears use suture to secure the ruptured tendon back to its native attachment site and often retear due to improper enthesis regeneration. To reduce the occurrence of retear, our laboratory developed an engineered
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Advancing Perfusion Models: Dual-Vessel Ex Vivo Rat Liver Perfusion Based on a Clinical Setup. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-08-31 Felix Strobl,Julian Michelotto,Vanessa Muth,Simon Moosburner,Kristina Knaub,Maximilian Zimmer,Madhukar S Patel,Johann Pratschke,Igor M Sauer,Nathanael Raschzok,Joseph M G V Gassner
Normothermic ex vivo liver machine perfusion (NEVLP) has been developed to address the increasing organ shortage in liver transplantation, through optimal preservation, assessment, and conditioning of grafts from extended criteria donors. There remains a need to establish simple and standardized animal models that simulate clinical NEVLP to test novel therapies. Liver grafts from 36 Sprague-Dawley
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Cryopreservation Alters Tissue Structure and Improves Differentiation of Engineered Skeletal Muscle. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-08-10 Lauren Gapinske,Lindsay Clark,Lourdes Marinna Caro-Rivera,Rashid Bashir
Tissue-engineered skeletal muscle can play an important role in regenerative medicine, disease modeling, drug testing, as well as the actuation of biohybrid machines. As the applications of engineered muscle tissues expand, there exists a growing need to cryopreserve and store these tissues without impairing function. In a previous study, we developed a cryopreservation protocol in which engineered
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Call for Special Issue Papers: Artificial Intelligence in Tissue Engineering and Biology. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-07-19 Jason L Guo,Michael Januszyk,Michael T Longaker
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Reconstruction of Fibrocartilage with Fibrous Alignment of Type I Collagen in Scaffold-Free Manner. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-07-24 Seoyoung Jang,Jisoo Lee,Jin Gil Jeong,Tong In Oh,EunAh Lee
For functional reconstruction of fibrocartilage, it is necessary to reproduce the essential mechanical property exhibited by natural fibrocartilage. The distinctive mechanical property of fibrocartilage is originated from the specific histological features of fibrocartilage composed of highly aligned type I collagen (Col I) and an abundant cartilaginous matrix. While the application of tensile stimulation
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Aspects of a Suspended Bioprinting System Affect Cell Viability and Support Bath Properties. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-07-20 Adam M Navara,Yilan Xu,Marissa R Perez,Antonios G Mikos
Suspended hydrogel printing is a growing method for fabricating bioprinted hydrogel constructs, largely due to how it enables nonviscous hydrogel inks to be used in extrusion printing. In this work, a previously developed poly(N-isopropylacrylamide)-based thermogelling suspended bioprinting system was examined in the context of chondrocyte-laden printing. Material factors such as ink concentration
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The Inhibitory Effect of RADKPS on Pyroptosis of Nucleus Pulposus-Derived Mesenchymal Stem Cells. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-07-11 Chao Zhu,Qing Zhou,Liang Tang,Anwu Xuan,Cheng Xu,Zuqiang Wang,Dike Ruan
Intervertebral disc (IVD) degeneration (IDD) is a primary cause of low-back pain in people, which is associated with nucleus pulposus-derived mesenchymal stem cells (NPMSCs). In this study, the involvement of lipopolysaccharide (LPS) in the pyroptosis of NPMSCs was investigated. The effect of RADKPS on the pyroptosis of NPMSCs and the underlying mechanism behind the impact of RADKPS on the proliferative
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Call for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-24 Laura Bracaglia,Themis Kyriakides
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Triply Periodic Minimal Surface-Based Scaffolds for Bone Tissue Engineering: A Mechanical, In Vitro and In Vivo Study. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-06-19 Ekaterina Maevskaia,Julien Guerrero,Chafik Ghayor,Indranil Bhattacharya,Franz E Weber
Triply periodic minimal surfaces (TPMSs) are found to be promising microarchitectures for bone substitutes owing to their low weight and superior mechanical characteristics. However, existing studies on their application are incomplete because they focus solely on biomechanical or in vitro aspects. Hardly any in vivo studies where different TPMS microarchitectures are compared have been reported. Therefore
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An Engineered Dermal Substitute with Mesenchymal Stem Cells Enhances Cutaneous Wound Healing. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-06-15 Jundong Xie,Jinmei Wang,Xiaoxiao Wang,Min Chen,Bin Yao,Yankai Dong,Xiaosong Li,Qingyang Yang,Edward E Tredget,Ren-He Xu,Yaojiong Wu
The treatment of refractory cutaneous wounds remains to be a clinical challenge. There is growing evidence to show that mesenchymal stem cells (MSCs) have great potential in promoting wound healing. However, the therapeutic effects of MSCs are greatly dampened by their poor survival and engraftment in the wounds. To address this limitation, in this study, MSCs were grown into a collagen-glycosaminoglycan
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Combined Growth Factor Hydrogel Enhances Rotator Cuff Enthesis Healing in Rat But Not Sheep Model. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-06-16 Scott M Bolam,Mark F Zhu,Khoon S Lim,Subhajit Konar,Mark H Oliver,Emma Buckels,Brya G Matthews,Karen E Callon,Tim Woodfield,Josh Workman,A Paul Monk,Brendan Coleman,Jillian Cornish,Jacob T Munro,David S Musson
We hypothesized that a combined growth factor hydrogel would improve chronic rotator cuff tear healing in a rat and sheep model. Insulin-like growth factor 1, transforming growth factor β1, and parathyroid hormone were combined into a tyraminated poly-vinyl-alcohol (PVA-Tyr) hydrogel and applied directly at the enthesis. In total, 30 Sprague-Dawley rats and 16 Romney ewes underwent unilateral rotator
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Genome Engineering of Human Urine-Derived Stem Cells to Express Lactoferrin and Deoxyribonuclease. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-06-02 Zara Kenigsberg,Richard C Welch,Julie Bejoy,Felisha M Williams,Ruth Ann Veach,Isria Jarrett,Trevor K Thompson,Matthew H Wilson,Lauren E Woodard
Urine-derived stem cells (USCs) are adult kidney cells that have been isolated from a urine sample and propagated in tissue culture on gelatin-coated plates. Urine is a practical and completely painless source of cells for gene and cell therapy applications. We have isolated, expanded, and optimized transfection of USCs to develop regenerative therapies based on piggyBac transposon modification. USCs
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Magnetic and Biocompatible Polyurethane Nanofiber Biomaterial for Tissue Engineering. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-30 Joshua A Choe,Susheil Uthamaraj,Dan Dragomir-Daescu,Gurpreet S Sandhu,Brandon J Tefft
Recruitment of endothelial cells to cardiovascular device surfaces could solve issues of thrombosis, neointimal hyperplasia, and restenosis. Since current targeting strategies are often nonspecific, new technologies to allow for site-specific cell localization and capture in vivo are needed. The development of cytocompatible superparamagnetic iron oxide nanoparticles has allowed for the use of magnetism
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In Vivo Efficacy of a Novel, Sutureless Coaptation Device for Repairing Peripheral Nerve Defects. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-06-02 Geetanjali Bendale,Matt Smith,Lida Daniel,Isabelle deBruler,Mariana Fernandes Gragnani,Ryan Clement,Jules McNeice,Fletcher Griffitts,Maximilian Sonntag,Jack Griffis,Isaac Clements,Jonathan Isaacs
Although microsuture neurorrhaphy is the accepted clinical standard treatment for severed peripheral nerves, this technique requires microsurgical proficiency and still often fails to provide adequate nerve approximation for effective regeneration. Entubulation utilizing commercially available conduits may enhance the technical quality of the nerve coaptation and potentially provide a proregenerative
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Characterization of the Temporomandibular Joint Disc Complex in the Yucatan Minipig. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-29 Ryan P Donahue,Eston G Kallins,Jerry C Hu,Kyriacos A Athanasiou
The temporomandibular joint (TMJ) disc complex (i.e., the TMJ disc and its six attachments) is crucial to everyday functions such as mastication and speaking. The TMJ can be afflicted by many conditions, including disc displacement and defects. Pathologies of the TMJ disc complex most commonly present first as anterior disc displacement, which the field hypothesizes may implicate the two posterior
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A Biomimetic Approach Utilizing Pulsatile Perfusion Generates Contractile Vascular Grafts. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-23 Cole Knox,Keren Garcia,Jade Tran,Sean M Wilson,Arlin B Blood,Mary Kearns-Jonker,Timothy P Martens
Surgical implantation of decellularized cadaveric arteries is routinely used to treat right-sided congenital cardiac lesions. These acellular conduits lack the capacity for somatic growth and are prone to stenosis and calcification, necessitating multiple operations throughout childhood. Islet-1+ cardiovascular progenitor cells (CPCs) have demonstrated the capacity for differentiation into all cell
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Tissue-Engineered Hepatocyte Sheets Supplemented with Adipose-Derived Stem Cells. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-12 Seiko Suzushino,Naoya Sato,Teruhide Ishigame,Ryo Okada,Yasuhide Kofunato,Junichiro Watanabe,Makoto Muto,Shigeyuki Tsukida,Atsushi Nishimagi,Takashi Kimura,Akira Kenjo,Satoshi Waguri,Shigeru Marubashi
The ability to engineer biologically viable hepatocytes and tissue matrices with long-term functional maintenance has attracted considerable interest in the fields of hepatocyte transplantation and liver tissue engineering. Here, newly developed hepatocyte sheets supplemented with adipose-derived stem cells (ADSCs) were evaluated to assess the effects of ADSCs on hepatocyte function and engraftment
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Cultivation and Transplantation of Three-Dimensional Skins with Laser-Processed Biodegradable Membranes. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-12 Yasuyuki Tsunoi,Izumi Takayama,Naonari Kondo,Yo Nagano,Hiromi Miyazaki,Satoko Kawauchi,Mitsuru Akashi,Daizoh Saitoh,Mitsuhiro Terakawa,Shunichi Sato
For the treatment of irreversible, extensive skin damage, artificial skins or cultured skins are useful when allogeneic skins are unavailable. However, most of them lack vasculature, causing delayed perfusion and hence delay or failure in engraftment of the tissues. We previously developed a prevascularized three-dimensional (3D) cultured skin based on the layer-by-layer cell coating technique (LbL-3D
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Small-Caliber Vascular Grafts Engineered from Decellularized Leaves and Cross-Linked Gelatin. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-05 Nicole Gorbenko,Gianna Rinaldi,Amalia Sanchez,Nick Merna
Despite advances in vascular replacement and repair, fabricating small-diameter vascular grafts with low thrombogenicity and appropriate tissue mechanics remains a challenge. A wide range of platforms have been developed to use plant-derived scaffolds for various applications. Unlike animal tissue, plants are primarily composed of cellulose which can offer a promising, nonthrombogenic alternative capable
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Enzyme-Mediated Nerve Growth Factor Release from Nanofibers Using Gelatin Microspheres. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-05-09 Elizabeth A Mays,Eric B Ellis,Zahin Hussain,Prahlad Parajuli,Harini G Sundararaghavan
Spinal cord injury is a complex environment, with many conflicting growth factors present at different times throughout the injury timeline. Delivery of multiple growth factors has received mixed results, highlighting a need to consider the timing of delivery for possibly antagonistic growth factors. Cell-mediated degradation of delivery vehicles for delayed release of growth factors offers an attractive
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Engineering Alignment Has Mixed Effects on Human Induced Pluripotent Stem Cell Differentiated Cardiomyocyte Maturation. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-04-04 Nikhith G Kalkunte,Talia E Delambre,Sogu Sohn,Madison Pickett,Sapun Parekh,Janet Zoldan
The potential of human induced pluripotent stem cell differentiated cardiomyocytes (hiPSC-CMs) is greatly limited by their functional immaturity. Strong relationships exist between cardiomyocyte (CM) structure and function, leading many in the field to seek ways to mature hiPSC-CMs by culturing on biomimetic substrates, specifically those that promote alignment. However, these in vitro models have
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Exosome Cell Origin Affects In Vitro Markers of Tendon Repair in Ovine Macrophages and Tenocytes. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-03-27 Devin von Stade,Melinda Meyers,James Johnson,Ted T Schlegel,Anthony Romeo,Daniel Regan,Kirk McGilvray
Tendon injuries and disease are resistant to surgical repair; thus, adjunct therapies are widely investigated, especially mesenchymal stromal cells (MSCs) and, more recently, their extracellular vesicles (MSCdEVs), for example, exosomes. Thought to act on resident and infiltrating immune cells, the role of MSCdEVs in paracrine signaling is of great interest. This study investigated how MSCdEVs differ
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2023 Lifetime Achievement Award of Tissue Engineering and Regenerative Medicine International Society-Americas: Kyriacos A. Athanasiou, PhD. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-03-01 Kyriacos A Athanasiou
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Highly Porous Gas-Blown Hydrogels for Direct Cell Encapsulation with High Cell Viability. Tissue Eng. Part A (IF 4.1) Pub Date : 2023-03-27 Henry T Beaman,Mary Beth B Monroe
Cell transplant therapies show potential as treatments for a large number of diseases. The encapsulation of cells within hydrogels is often used to mimic the extracellular matrix and protect cells from the body's immune response. However, cell encapsulation can be limited in terms of both scaffold size and cell viability due to poor nutrient and waste transport throughout the bulk of larger volume
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Hyperglycemic Conditions Enhance the Mechanosensitivity of Proinflammatory RAW264.7 Macrophages Tissue Eng. Part A (IF 4.1) Pub Date : 2023-02-03 Courtney D. Johnson, Decklan Fischer, Ian Michael Smith, Helim Aranda-Espinoza, John P. Fisher
Macrophages are a primary contributor to the orchestration and severity of the foreign body response. As phagocytes and antigen-presenting cells, macrophages engage foreign objects, producing chemokines, degrading enzymes, and proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Encapsulated islet transplantation (EIT) is a return of function therapy in
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The UCMSC-bFGF/Scaffold System Accelerates the Healing of the Uterine Full-Thickness Injury Tissue Eng. Part A (IF 4.1) Pub Date : 2023-02-02 Limin Wang, Simin Yao, Feifei Huang, Haining Lv, Dan Liu, Tianyun Gao, Bin Wang, Zhenhua Zhou, Chenrui Cao, Qi Zhu, Qiao Weng, Guangfeng Zhao, Yali Hu
Severe uterine injury is a major cause of endometrial scar formation and female infertility. At present, the methods for accelerating injured uterine healing are still lacking. Genetic engineering modification of mesenchymal stem cells (MSCs) has been shown great promise in preclinical studies on regeneration. Here, we constructed a type of umbilical cord MSCs (UC-MSCs) with overexpressed basic fibroblast
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The Effect of Dosing of Stromal Cell-Derived Factor 1 on Anal Sphincter Regeneration Tissue Eng. Part A (IF 4.1) Pub Date : 2023-01-27 Li Sun, Alanna Billups, Anna Rietsch, Margot S. Damaser, Massarat Zutshi
Aim: The aim of this study is to investigate if a high dose of the stromal cell-derived factor-1 (SDF-1) plasmid improves outcome in a minipig model of chronic anal sphincter injury.
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ALK-5 Inhibitors for Efficient Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells Tissue Eng. Part A (IF 4.1) Pub Date : 2023-01-27 Shawn P. Grogan, Nicholas E. Glembotski, Darryl D. D'Lima
Objectives: Successful tissue regeneration requires a clinically viable source of mesenchymal stem cells (MSCs). We explored activin receptor-like kinase (ALK)-5 inhibitors to rapidly derive an MSC-like phenotype with high cartilage forming capacity from a xeno-free human embryonic cell line.
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Tricultured Cell Sheets Develop into Functional Pancreatic Islet Tissue with a Vascular Network Tissue Eng. Part A (IF 4.1) Pub Date : 2023-01-27 Jun Homma, Hidekazu Sekine, Tatsuya Shimizu
Methods to induce islet β-cells from induced pluripotent stem cells or embryonic stem cells have been established. However, islet β-cells are susceptible to apoptosis under hypoxic conditions, so the technique used to transplant β-cells must maintain the viability of cells in vivo. This study describes the development of a tricultured cell sheet, which was made by coculturing islet β-cells, vascular
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Fast Degradable Calcium Phosphate Cement for Maxillofacial Bone Regeneration Tissue Eng. Part A (IF 4.1) Pub Date : 2023-01-27 Bart van Oirschot, Antonios G. Mikos, Qian Liu, Jeroen J.J.P. van den Beucken, John A. Jansen
The aim of this preclinical study was to test the applicability of calcium phosphate cement (CPC)-poly(lactic-co-glycolic acid) (PLGA)-carboxymethylcellulose (CMC) as a bone substitute material for guided bone regeneration (GBR) procedures in a clinically relevant mandibular defect model in minipigs. In the study, a predicate device (i.e., BioOss®) was included for comparison. Critical-sized circular