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Increasing glycolysis by deletion of kcs1 and arg82 improved S-adenosyl- l -methionine production in Saccharomyces cerevisiae AMB Express (IF 2.499) Pub Date : 2021-01-19 Hailong Chen, Nianqing Zhu, Yan Wang, Xinxin Gao, Yuhe Song, Jia Zheng, Jiaping Peng, Xin Zhang
Reprogramming glycolysis for directing glycolytic metabolites to a specific metabolic pathway is expected to be useful for increasing microbial production of certain metabolites, such as amino acids, lipids or considerable secondary metabolites. In this report, a strategy of increasing glycolysis by altering the metabolism of inositol pyrophosphates (IPs) for improving the production of S-adenosyl-l-methionine
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Feruloyl esterase (FAE-1) sourced from a termite hindgut and GH10 xylanases synergy improves degradation of arabinoxylan AMB Express (IF 2.499) Pub Date : 2021-01-19 Mpho S. Mafa, Samkelo Malgas, Brett I. Pletschke
Cereal feedstocks have high arabinoxylan content as their main hemicellulose, which is linked to lignin by hydroxycinnamic acids such as ferulic acid. The ferulic acid is linked to arabinoxylan by ester bonds, and generally, the high substitution of ferulic acid leads to a loss of activity of xylanases targeting the arabinoxylan. In the current study, a feruloyl esterase (FAE-1) from a termite hindgut
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Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota AMB Express (IF 2.499) Pub Date : 2021-01-19 Jia Wang, Jiawei Liang, Yonghong Li, Lingmin Tian, Yongjun Wei
Xylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to
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New evidence for a hydroxylation pathway for anaerobic alkane degradation supported by analyses of functional genes and signature metabolites in oil reservoirs AMB Express (IF 2.499) Pub Date : 2021-01-12 Li-Bin Shou, Yi-Fan Liu, Jing Zhou, Zhong-Lin Liu, Lei Zhou, Jin-Feng Liu, Shi-Zhong Yang, Ji-Dong Gu, Bo-Zhong Mu
Microbial degradation of recalcitrant alkanes under anaerobic conditions results in the accumulation of heavy oil fraction in oil reservoirs. Hydroxylation of alkanes is an important activation mechanism under anaerobic conditions, but the diversity and distribution of the responsible microorganisms in the subsurface environment are still unclear. The lack of functional gene polymerase chain reaction
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Development of Chinese chestnut whiskey: yeast strains isolation, fermentation system optimization, and scale-up fermentation AMB Express (IF 2.499) Pub Date : 2021-01-11 Wanzhen Li, Cuie Shi, Jiaquan Guang, Fei Ge, Shoubao Yan
In this study, we used Chinese chestnut as the main raw material to develop a novel type of whiskey. First, 16 yeasts were isolated and identified for producing aroma using olfactory plate assay. Of these, we screened nine yeast strains based on their fermentation capacity, aroma profile, and sensory evaluation. The results demonstrated the combination of strains HN006 (Saccharomyces cerevisiae) and
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Application of a roller conveyor type plasma disinfection device with fungus-contaminated citrus fruits AMB Express (IF 2.499) Pub Date : 2021-01-09 Akikazu Sakudo, Yoshihito Yagyu
Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressure dielectric barrier discharge (APDBP) plasma on a set of rollers. Penicillium venetum
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Stress tolerance of Xerocomus badius and its promotion effect on seed germination and seedling growth of annual ryegrass under salt and drought stresses AMB Express (IF 2.499) Pub Date : 2021-01-07 Binghua Liu, Xinghong Liu, Fangchun Liu, Hailin Ma, Bingyao Ma, Lin Peng
Comparative evaluations were conducted to assess the effects of different pH levels, NaCl-induced salt stress, and PEG-induced drought stress on the mycelial growth of Xerocomus badius. The results showed that X. badius mycelium grew well at a wide pH range of 5.00 ~ 9.00. Although the mycelium remained viable, mycelial growth of X. badius was significantly inhibited with increasing salt and drought
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Acceleration of emergence of E. coli antibiotic resistance in a simulated sublethal concentration of copper and tetracycline co-contaminated environment AMB Express (IF 2.499) Pub Date : 2021-01-07 Jinmei Li, Irfan Ali Phulpoto, Guilong Zhang, Zhisheng Yu
An environment co-contaminated with metals and antibiotics ultimately exposes bacteria to these metals and antibiotics simultaneously. This study aims to explore the efficacy of sublethal concentrations of copper ions contaminated with tetracycline regarding antibiotic resistance in a sensitive strain of E. coli K12. The study proved that a copper ions and tetracycline co-contaminated environment could
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Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae AMB Express (IF 2.499) Pub Date : 2021-01-07 Yoko Hirono-Hara, Miyuu Yui, Kiyotaka Y. Hara
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Microbial keratinase and the bio-economy: a three-decade meta-analysis of research exploit AMB Express (IF 2.499) Pub Date : 2021-01-07 Nonso E. Nnolim, Uchechukwu U. Nwodo
Microbial keratinase research has been on an upward trajectory due to the robustness and efficiency of the enzyme toward various green technological processes that promote economic development and environmental sustainability. A compendium of research progression and advancement within the domain was achieved through a bibliometric study to understand the trend of research productivity, scientific
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Effect of different sweeteners on the oral microbiota and immune system of Sprague Dawley rats AMB Express (IF 2.499) Pub Date : 2021-01-06 Xi Cheng, Xiurong Guo, Feihong Huang, Hui Lei, Quan Zhou, Can Song
Sucrose, xylose, and saccharin are commonly used beverage additives and long-term consumption of these compounds inevitably affects the oral immune system and the composition of oral microbiomes. In this study, we used 24 Sprague Dawley rats divided into four groups, i.e., sucrose, saccharin, xylose, or pure water treated over an eight week period to evaluate any changes in the composition, community
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Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies AMB Express (IF 2.499) Pub Date : 2021-01-06 Manyuan Bai, Rui Wang, Shiqi Sun, Yun Zhang, Hu Dong, Huichen Guo
Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens.
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Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing AMB Express (IF 2.499) Pub Date : 2021-01-06 Zongfu Hu, Deying Ma, Huaxin Niu, Jie Chang, Jianhua Yu, Qing Tong, Shuguo Li
The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling
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Visual multiple cross displacement amplification for the rapid identification of S. agalactiae immediately from vaginal and rectal swabs AMB Express (IF 2.499) Pub Date : 2021-01-06 Xueqin Cheng, Zhiqian Dou, Jing Yang, Dexi Liu, Yulong Gu, Fenglin Cai, Xiaobing Li, Meifang Wang, Yijun Tang
Streptococcus agalactiae (S. agalactiae) is an important pathogen that can lead to neonatus and mother infection. The current existing techniques for the identification of S. agalactiae are limited by accuracy, speed and high-cost. Therefore, a new multiple cross displacement amplification (MCDA) assay was developed for test of the target pathogen immediately from vaginal and rectal swabs. MCDA primers
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Isolation and characterisation of Methylocystis spp. for poly-3-hydroxybutyrate production using waste methane feedstocks AMB Express (IF 2.499) Pub Date : 2021-01-06 Bashir L. Rumah, Christopher E. Stead, Benedict H. Claxton Stevens, Nigel P. Minton, Alexander Grosse-Honebrink, Ying Zhang
Waste plastic and methane emissions are two anthropogenic by-products exacerbating environmental pollution. Methane-oxidizing bacteria (methanotrophs) hold the key to solving these problems simultaneously by utilising otherwise wasted methane gas as carbon source and accumulating the carbon as poly-3-hydroxybutyrate, a biodegradable plastic polymer. Here we present the isolation and characterisation
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Agricultural by-products and oyster shell as alternative nutrient sources for microbial sealing of early age cracks in mortar AMB Express (IF 2.499) Pub Date : 2021-01-06 Minyoung Hong, Indong Jang, Yongjun Son, Chongku Yi, Woojun Park
Bio-concrete using bacterially produced calcium carbonate can repair microcracks but is still relatively expensive due to the addition of bacteria, nutrients, and calcium sources. Agricultural by-products and oyster shells were used to produce economical bio-concrete. Sesame meal was the optimal agricultural by-product for low-cost spore production of the alkaliphilic Bacillus miscanthi strain AK13
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Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum AMB Express (IF 2.499) Pub Date : 2021-01-06 Daniel P. Roberts, Kaitlyn Selmer, Robert Lupitskyy, Clifford Rice, Jeffrey S. Buyer, Jude E. Maul, Dilip K. Lakshman, Jorge DeSouza
Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass
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Ammonium nitrate regulated the color characteristic changes of pigments in Monascus purpureus M9 AMB Express (IF 2.499) Pub Date : 2021-01-04 Di Chen, Yurong Wang, Mianhua Chen, Pei Fan, Guiling Li, Changlu Wang
Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concentration of intracellular pigments was significantly decreased when subjected to AN
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The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum ( Sciaenops ocellatus ) using PCR AMB Express (IF 2.499) Pub Date : 2021-01-04 Pham Thi Hai Yen, Nguyen Quang Linh, Nguyen Duy Quynh Tram
Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%
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Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors AMB Express (IF 2.499) Pub Date : 2021-01-03 Barbara G. Paes, Andrei Stecca Steindorff, Eduardo F. Formighieri, Ildinete Silva Pereira, João Ricardo M. Almeida
The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance
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Screening and selection strategy for the establishment of biosimilar to trastuzumab-expressing CHO-K1 cell lines AMB Express (IF 2.499) Pub Date : 2021-01-03 Thailin Lao-Gonzalez, Alexi Bueno-Soler, Arnelys Duran-Hernandez, Katya Sosa-Aguiar, Luis Eduardo Hinojosa-Puerta, Tays Hernandez-Garcia, Kathya Rashida de la Luz-Hernandez, Julio Palacios-Oliva, Tammy Boggiano-Ayo
The high prices of biopharmaceuticals or biologics used in the treatment of many diseases limit the access of patients to these novel therapies. One example is the monoclonal antibody trastuzumab, successfully used for breast cancer treatment. An economic alternative is the generation of biosimilars to these expensive biopharmaceuticals. Since antibody therapies may require large doses over a long
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Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi AMB Express (IF 2.499) Pub Date : 2020-12-21 Antonia Werner, Kolja L. Otte, Gertrud Stahlhut, Stefanie Pöggeler
The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell biology and biochemical applications in filamentous fungi. We report the generation
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The protective role of intracellular glutathione in Saccharomyces cerevisiae during lignocellulosic ethanol production AMB Express (IF 2.499) Pub Date : 2020-12-17 Vijayendran Raghavendran, Christian Marx, Lisbeth Olsson, Maurizio Bettiga
To enhance the competitiveness of industrial lignocellulose ethanol production, robust enzymes and cell factories are vital. Lignocellulose derived streams contain a cocktail of inhibitors that drain the cell of its redox power and ATP, leading to a decrease in overall ethanol productivity. Many studies have attempted to address this issue, and we have shown that increasing the glutathione (GSH) content
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Gα3 subunit Thga3 positively regulates conidiation, mycoparasitism, chitinase activity, and hydrophobicity of Trichoderma harzianum AMB Express (IF 2.499) Pub Date : 2020-12-17 Jie Ding, Jie Mei, Pei Huang, Ying Tian, Yao Liang, Xiliang Jiang, Mei Li
Heterotrimeric G-proteins are key elements of signal transduction pathways, which participate in regulating multiple biological processes in fungi including growth, conidiation, antagonism, and mycoparasitism. Among G protein subunits, Gα3 showed diverse regulatory functions in different fungi. In this study, we cloned a Gα3 subunit coding gene thga3 from T. harzianum Th33 that can antagonize Rhizoctonia
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Pretreatment with probiotics ameliorate gut health and necrotic enteritis in broiler chickens, a substitute to antibiotics AMB Express (IF 2.499) Pub Date : 2020-12-17 Danish Sharafat Rajput, Dong Zeng, Abdul Khalique, Samia Sharafat Rajput, Hesong Wang, Ying Zhao, Ning Sun, Xueqin Ni
Necrotic enteritis (NE) is being considered as one of the most important intestinal diseases in the recent poultry production systems, which causes huge economic losses globally. NE is caused by Clostridium perfringens, a pathogenic bacterium, and normal resident of the intestinal microflora of healthy broiler chickens. Gastrointestinal tract (GIT) of broiler chicken is considered as the most integral
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Correction to: Synergistic interaction of eugenol and antimicrobial drugs in eradication of single and mixed biofilms of Candida albicans and Streptococcus mutans AMB Express (IF 2.499) Pub Date : 2020-12-14 Huma Jafri, Gopa Banerjee, Mohd Sajjad Ahmad Khan, Iqbal Ahmad, Hussein Hasan Abulreesh, Abdulla Safar Althubiani
An amendment to this paper has been published and can be accessed via the original article.
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Repeatability and reproducibility of the wzi high resolution melting-based clustering analysis for Klebsiella pneumoniae typing AMB Express (IF 2.499) Pub Date : 2020-12-14 Ajay Ratan Pasala, Matteo Perini, Aurora Piazza, Simona Panelli, Domenico Di Carlo, Cristian Loretelli, Alessandra Cafiso, Sonia Inglese, Floriana Gona, Daniela Maria Cirillo, Gian Vincenzo Zuccotti, Francesco Comandatore
High resolution melting (HRM) is a fast closed-tube method for nucleotide variant scanning applicable for bacterial species identification or molecular typing. Recently a novel HRM-based method for Klebsiella pneumoniae typing has been proposed: it consists of an HRM protocol designed on the capsular wzi gene and an HRM-based algorithm of strains clustering. In this study, we evaluated the repeatability
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A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea AMB Express (IF 2.499) Pub Date : 2020-12-14 Yue Zhang, Youhui Gao, Zehui Zheng, Xingyao Meng, Yafan Cai, Jianbin Liu, Yuegao Hu, Shuangdui Yan, Xiaofen Wang
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Oral administration of recombinant Bacillus subtilis spores expressing mutant staphylococcal enterotoxin B provides potent protection against lethal enterotoxin challenge AMB Express (IF 2.499) Pub Date : 2020-12-14 Zhile Xiong, Jialiang Mai, Fei Li, Bingshao Liang, Shuwen Yao, Zhuwei Liang, Chao Zhang, Fei Gao, Xiaolan Ai, Jielin Wang, Yan Long, Min Yang, Sitang Gong, Zhenwen Zhou
Pathogenicity of Staphylococcus aureus is induced by staphylococcal enterotoxin B (SEB). A mutant form of SEB (mSEB) is immunogenic as well as less toxic. Recombinant mSEB and SEB were expressed in pET28a prokaryotic plasmids. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in mSEB-stimulated macrophages were lower than those in SEB-stimulated macrophages (p < 0.001, p < 0.01 respectively)
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High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli AMB Express (IF 2.499) Pub Date : 2020-12-11 Bing Yan Liu, Bin Gao, Meng Zhi Liu, Ting Ting Zhang, Bao Shan Liu, Ze Liang Chen
Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results
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Construction of a mApple-D6A3-mediated biosensor for detection of heavy metal ions AMB Express (IF 2.499) Pub Date : 2020-12-07 Yangyang Ji, Feifei Guan, Xin Zhou, Xiaoqing Liu, Ningfeng Wu, Daling Liu, Jian Tian
Pollution of heavy metals in agricultural environments is a growing problem to the health of the world’s human population. Green, low-cost, and efficient detection methods can help control such pollution. In this study, a protein biosensor, mApple-D6A3, was built from rice-derived Cd2+-binding protein D6A3 fused with the red fluorescent protein mApple at the N-terminus to detect the contents of heavy
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Effects of Bacillus subtilis on growth performance, serum parameters, digestive enzyme, intestinal morphology, and colonic microbiota in piglets AMB Express (IF 2.499) Pub Date : 2020-12-02 Bo Deng, Jie Wu, Xiaohui Li, Cheng Zhang, Xiaoming Men, Ziwei Xu
The present study was conducted to investigate effects of Bacillus subtilis on growth performance, serum parameters, digestive enzymes, intestinal morphology, and colonic microbiota in piglets. A total of 72 piglets were weighed and randomly allotted into three treatments (four replication pens per treatment with six piglets/pen) for a 28-day experiment. The dietary treatments were as follows: basal
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GermiX: A skin friendly hand sanitizer with prolonged effectivity against pathogenic bacteria AMB Express (IF 2.499) Pub Date : 2020-12-01 Acharya Balkrishna, Kanchan Singh, Hoshiyar Singh, Swati Haldar, Anurag Varshney
COVID-19 pandemic has almost made hand sanitization a ritual resulting in a steep increase in the frequency of hand sanitization and an unprecedented surge in demand for hand sanitizers. In fact, several governments had to ration hand sanitizers in the retail outlets and over the counter chemist shops. Additionally, Indian government has put a cap on the prices of hand sanitizers. Currently, large
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Effects of corn steep liquor on β-poly( l -malic acid) production in Aureobasidium melanogenum AMB Express (IF 2.499) Pub Date : 2020-12-01 Genan Wang, Bingyi Shi, Pan Zhang, Tingbin Zhao, Haisong Yin, Changsheng Qiao
β-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production
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Correction to: Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel‑based DNA profiling method AMB Express (IF 2.499) Pub Date : 2020-11-26 Ayomi Dilhari, Asanga Sampath, Chinthika Gunasekara, Neluka Fernando, Deepaka Weerasekara, Chris Sissons, Andrew McBain, Manjula Weerasekera
An amendment to this paper has been published and can be accessed via the original article.
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Utilization of a recombinant defensin from Maize ( Zea mays L.) as a potential antimicrobial peptide AMB Express (IF 2.499) Pub Date : 2020-11-25 Najla Amin T. Al Kashgry, Hussein H. Abulreesh, Iman A. El-Sheikh, Yaser A. Almaroai, Reda Salem, Ismail Mohamed, Fatma R. Waly, Gamal Osman, Mahmoud S. M. Mohamed
The search for effective and bioactive antimicrobial molecules to encounter the medical need for new antibiotics is an encouraging area of research. Plant defensins are small cationic, cysteine-rich peptides with a stabilized tertiary structure by disulfide-bridges and characterized by a wide range of biological functions. The heterologous expression of Egyptian maize defensin (MzDef) in Escherichia
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Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal AMB Express (IF 2.499) Pub Date : 2020-11-24 Jinghua Ruan, Wujun Wang, Tiyin Zhang, Teng Zheng, Jing Zheng, Shiyu Yu, Daojin Yu, Yifan Huang
Salmonella spp. is a high-risk bacterial pathogen that is monitored in imported animal-derived feedstuffs. Serratia fonticola is the bacterial species most frequently confused with Salmonella spp. in traditional identification methods based on biochemical characteristics, which are time-consuming and labor-intensive, and thus unsuitable for daily inspection and quarantine work. In this study, we established
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Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera AMB Express (IF 2.499) Pub Date : 2020-11-11 Navdeep Kaur, Daljit Singh Arora
The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial potential and is optimized for physiochemical parameters to enhance the antimicrobial
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LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1 AMB Express (IF 2.499) Pub Date : 2020-11-11 Jun-Fang He, Du-Xin Jin, Xue-Gang Luo, Tong-Cun Zhang
Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range
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Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli AMB Express (IF 2.499) Pub Date : 2020-11-11 Yanfeng Chen, Wenjie Ke, Huabin Qin, Siwei Chen, Limei Qin, Ying Yang, Hui Yu, Yuansheng Tan
This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the
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HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways AMB Express (IF 2.499) Pub Date : 2020-11-10 Xin Zhou, Ningou Huang, Wenxin Chen, Tang Xiaoling, Behnam Mahdavi, Amir Raoofi, Davood Mahdian, Hadi Atabati
Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content
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Reclassification of Chromobacterium violaceum ATCC 31532 and its quorum biosensor mutant CV026 to Chromobacterium subtsugae AMB Express (IF 2.499) Pub Date : 2020-11-07 Alisha M. Harrison, Scott D. Soby
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Structural and Physicochemical Characterization of Rhamnolipids produced by Pseudomonas aeruginosa P6 AMB Express (IF 2.499) Pub Date : 2020-11-04 Ghadir S. El-Housseiny, Khaled M. Aboshanab, Mohammad M. Aboulwafa, Nadia A. Hassouna
Rhamnolipids are important biosurfactants for application in bioremediation, enhanced oil recovery, pharmaceutical, and detergent industry. In this study, rhamnolipids extracted from P. aeruginosa P6 were characterized to determine their potential fields of application. Thin-layer chromatographic analysis of the produced rhamnolipids indicated the production of two homologues: mono- and di-rhamnolipids
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Response of alfalfa growth to arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria under different phosphorus application levels AMB Express (IF 2.499) Pub Date : 2020-11-03 Junying Liu, Xuanshuai Liu, Qianbing Zhang, Shengyi Li, Yanliang Sun, Weihua Lu, Chunhui Ma
Alfalfa (Medicago sativa L.) is an important forage legume in farming and animal husbandry systems. This study assessed the effects of arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing bacteria (PSB) on alfalfa growth under different phosphorus application levels. In this experiment, a complete randomized block design was used. The following four bacterial applications were used: inoculation
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Identification of stress-responsive transcription factors with protein-bound Escherichia coli genomic DNA libraries AMB Express (IF 2.499) Pub Date : 2020-11-02 Xianqiang Li, Xin Jiang, Meiying Xu, Yun Fang, Yan Wang, Guoping Sun, Jun Guo
Bacteria promoters along with operators are crucial elements in the control of gene expression in microbes in response to environmental stress changes. A genome-wide promoter DNA regulatory library is in demand to be developed for a microbe reporter method to monitor the existence of any given environmental stress substance. In this study, we utilized Escherichia coli (E. coli) as a model system for
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Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia AMB Express (IF 2.499) Pub Date : 2020-11-01 Maroua Oueslati, Magdalena Mulet, Mohamed Zouaoui, Charlotte Chandeysson, Jorge Lalucat, Mohamed Rabeh Hajlaoui, Odile Berge, Elena García-Valdés, Najla Sadfi-Zouaoui
The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping
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Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20 AMB Express (IF 2.499) Pub Date : 2020-10-31 Zhiyong Liu, Kai Dang, Cunzhi Li, Junhong Gao, Hong Wang, Yongchao Gao, Bin Zhao, Peng Fan, Airong Qian
Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade CL-20 with desirable efficiency. Metagenomic sequencing methods were performed to
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Effect of Broussonetia papyrifera silage on the serum indicators, hindgut parameters and fecal bacterial community of Holstein heifers AMB Express (IF 2.499) Pub Date : 2020-10-31 Hanchen Tian, Yiye Chen, Ni Zhu, Yongqing Guo, Ming Deng, Guangbin Liu, Yaokun Li, Dewu Liu, Baoli Sun
This study investigated the effects of substitution of whole corn silage (WCS) with Broussonetia papyrifera silage (BPS) in different ratios on the serum indicators, hindgut fermentation parameters (pH, ammoniacal nitrogen, and volatile fatty acids), and fecal bacterial community of Holstein heifers. Sixteen heifers (8-month-old, 220 ± 30 kg) were randomly divided into four treatments according to
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Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2 AMB Express (IF 2.499) Pub Date : 2020-10-30 Xiaomeng Pei, Junchi Liu, Mingxing Liu, Hong Zhou, Xiaomin Wang, Hongjie Fan
Streptococcus suis serotype 2 (SS2) is a serious zoonotic pathogen; it can lead to symptoms of streptococcal toxic shock syndrome (STSS) in humans and sepsis in pigs, and poses a great threat to public health. The SS2 MetQ gene deletion strain has attenuated antiphagocytosis, although the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope
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Biodegradation and metabolic pathway of fenvalerate by Citrobacter freundii CD-9 AMB Express (IF 2.499) Pub Date : 2020-10-30 Jie Tang, Dan Lei, Min Wu, Qiong Hu, Qing Zhang
Fenvalerate is a pyrethroid insecticide with rapid action, strong targeting, broad spectrum, and high efficiency. However, continued use of fenvalerate has resulted in its widespread presence as a pollutant in surface streams and soils, causing serious environmental pollution. Pesticide residues in the soil are closely related to food safety, yet little is known regarding the kinetics and metabolic
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Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses AMB Express (IF 2.499) Pub Date : 2020-10-29 Xin-Jian Li, Mingyu Wang, Yahui Xue, Dongdong Duan, Cong Li, Xuelei Han, Kejun Wang, Ruimin Qiao, Xiu-Ling Li
Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via
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Pseudomonas species isolated via high-throughput screening significantly protect cotton plants against verticillium wilt AMB Express (IF 2.499) Pub Date : 2020-10-28 Xiaoyuan Tao, Hailin Zhang, Mengtao Gao, Menglin Li, Ting Zhao, Xueying Guan
Verticillium wilt (VW) caused by Verticillium dahliae is a devastating soil-borne disease that causes severe yield losses in cotton and other major crops worldwide. Here we conducted a high-throughput screening of isolates recovered from 886 plant rhizosphere samples taken from the three main cotton-producing areas of China. Fifteen isolates distributed in different genera of bacteria that showed inhibitory
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Biohydrogen production by Chlorella vulgaris and Scenedesmus obliquus immobilized cultivated in artificial wastewater under different light quality AMB Express (IF 2.499) Pub Date : 2020-10-27 Alejandro Ruiz-Marin, Yunuen Canedo-López, Paolah Chávez-Fuentes
The algal biotechnology together with the wastewater treatment can contribute to the production of renewable energies such as bioethanol, biodiesel and biohydrogen and solve many of the challenges currently facing the shortage of fossil fuels and environmental impacts. Hydrogen as the cleanest source of energy is a promising alternative to conventional fossil fuels. Among different technologies for
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Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer AMB Express (IF 2.499) Pub Date : 2020-10-23 Dayana Morales-Borrell, Nemecio González-Fernández, Néstor Mora-González, Carlos Pérez-Heredia, Ana Campal-Espinosa, Eddy Bover-Fuentes, Eladio Salazar-Gómez, Yissel Morales-Espinosa
Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica
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Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt AMB Express (IF 2.499) Pub Date : 2020-10-23 Yi Zhang, Cheng Tian, Jiling Xiao, Lin Wei, Yun Tian, Zhihuai Liang
Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. niveum (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, Trichoderma asperellum (T. asperellum) M45a was shown to be an effective biocontrol agent against FW. In a pot experiment, the application of 105 cfu/g of T. asperellum M45a granules had an improved control effect on FW during the blooming period
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Production and composition of Pleurotus ostreatus cultivated on Lithovit ® -Amino25 supplemented spent substrate AMB Express (IF 2.499) Pub Date : 2020-10-21 Layla Naim, Mohammed A. Alsanad, Nidal Shaban, Zeina El Sebaaly, Sami Abou Fayssal, Youssef N. Sassine
Supplementation of the spent oyster substrate enhances its nutritional properties to produce a new mushroom cropping cycle. The study investigated the potential of a nano-fertilizer (Lithovit®-Amino25) with an admixture of 25% l-amino acids on Pleurotus ostreatus production, proteins, and amino acid contents. The product applied at spawning (t1), after the first harvest (t2), and at both timings (t3)
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Enhancement of the antioxidant and skin permeation properties of eugenol by the esterification of eugenol to new derivatives AMB Express (IF 2.499) Pub Date : 2020-10-19 Edyta Makuch, Anna Nowak, Andrzej Günther, Robert Pełech, Łukasz Kucharski, Wiktoria Duchnik, Adam Klimowicz
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rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay AMB Express (IF 2.499) Pub Date : 2020-10-19 Antonio Pedro Fróes de Farias, José Tadeu Raynal Rocha Filho, Silvana Beutinger Marchioro, Luan Santana Moreira, Andressa Souza Marques, Maria da Conceição Aquino de Sá, Antonio Anderson dos Santos Oliveira, Maria Emília Alcântara, Ricardo Barros Mariutti, Raghuvir Krishnaswamy Arni, Soraya Castro Trindade, Roberto Meyer
Caseous lymphadenitis (CL) is a chronic infectious disease that affects sheep and goats. Many serological tests have been developed to detect the disease; one of the most widely used is the enzyme-linked immunosorbent assay (ELISA), due to its advantages, which include acceptable cost-effectiveness, applicability, sensitivity and specificity. ELISA formulations using recombinant proteins can exhibit
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Synergistic interaction of eugenol and antimicrobial drugs in eradication of single and mixed biofilms of Candida albicans and Streptococcus mutans AMB Express (IF 2.499) Pub Date : 2020-10-19 Huma Jafri, Gopa Banerjee, Mohd Sajjad Ahmad Khan, Iqbal Ahmad, Hussein Hasan Abulreesh, Abdullah Safar Althubiani
In vitro eradication of the C. albicans and S. mutans mixed biofilms by eugenol alone and in combination with the antimicrobial drugs. Previously characterized strains of C. albicans (CAJ-01 and CAJ-12) and S. mutans MTCC497 were used to evaluate the eradication of biofilms using XTT reduction assay, viability assay, time dependent killing assay and scanning electron microscopy (SEM). Synergistic interaction
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Characterization of volatile fatty-acid utilization in Escherichia coli aiming for robust valorisation of food residues AMB Express (IF 2.499) Pub Date : 2020-10-17