To establish cholyglycine (CG) detection via enzyme multiplied immunoassay technique (EMIT), glucose‐6‐phosphate dehydrogenase (G6PD) was used as a reporter enzyme to prepare hapten‐enzyme conjugate. Gel electrophoresis and UV scanning demonstrated that G6PD was successfully labeled with cholyglycine and CG‐G6PD conjugate was obtained. Furthermore, the effects of various parameters on the preparation

A high‐performance interdigitated electrode (IDE) biosensing surface was reported here by utilizing self‐assembled silica nanoparticle (SiNP). The modified surface was used to evaluate the complementation of hairpin forming region from Mitoxantrone resistance gene 7 (MXR7; liver cancer related short gene). The conjugated SiNPs on 3‐Aminopropyl Triethoxysilane functionalization were captured with probe

Urinary C‐terminal telopeptide fragment of type II collagen (uCTX‐II) has been reported as the efficient blood‐based biomarker for osteoarthritis, affects knees, hands, spine and hips. This study reports a sensing strategy with antibody conjugated gold nanoparticle (GNP) on Interdigitated electrode (IDE) to determine uCTX‐II. GNP‐antibody complex was chemically immobilized on IDE surface through the

The aim of this paper is to make the point on the fortieth years study on the β‐glycosidase from S. solfataricus. This enzyme represents one of the thermophilic biocatalysts the more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studies highlighted its broad substrate specificity for β‐D‐galacto‐, gluco‐ and fuco‐sides and showed

Gap junctional intercellular communication (GJIC) is the transfer of ions, metabolites and second messengers between neighboring cells through intercellular junctions. Connexin 43 (Cx43) was found to be the type of gap junction protein responsible for human granulosa cells (GCs) and oocyte communication, which is required for folliculogenesis and oocyte maturation. Bisphenol A (BPA), an estrogenic‐like

C‐Phycocyanin (C‐Pc), a photosynthetic pigment for use as a fluorescent indicator or in pharmaceutical, food, and cosmetic products, exists in a phycobilisome complex with allophycocyanin (APC), phycoerythrin (PE), and linker polypeptides. This heterogeneity makes it difficult to quantify phycobilisome composition in an ultraviolet‐visible (UV‐vis) spectrum. In this study, derivative analysis of UV‐vis

Recent years have witnessed a rapid increase in the application of enzymes for chemical synthesis and manufacturing, including the industrial‐scale synthesis of pharmaceuticals using multi‐enzyme processes. From an operational standpoint, these bioprocesses often require robust biocatalysts capable of tolerating high concentrations of organic solvents and possessing long shelf‐life stability. In this

As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV‐2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV‐2 diseases. However, there is currently a lack of simple and effective method for quantitative detection of CPV‐2 in vaccine. Therefore, this study aims to prepare an accurate method to

To prevent foodborne diseases and minimize their impacts, it is extremely important to develop a cost‐effective and efficient bacterial detection assay for diagnostics, particularly in resource‐poor settings. In this study, 16S rRNA from foodborne Salmonella was coupled with multiple HCR (hybridization chain reaction) concatemers and functionalized in a signal structure for lateral flow nucleic acid

COVID-19 threatens millions of lives, especially elderly population and people with chronic diseases including diabetes, hypertension, cancer, and cardiovascular diseases. Rapid and effective diagnoses are vital for the isolation of infected people and starting treatment immediately to stop the spread of COVID-19 virus. Bioinformatics techniques such as artificial intelligence should be used for collecting

Chronic obstructive pulmonary disease (COPD) is a chronic and devastating condition characterized by poor airflow and breath. Smoking and other environmental factors-caused inflammations triggered excessive autophagy of normal lung epithelial cells, eventually leading to impaired lung functions. Previous studies showed that ghrelin exhibited beneficial effects on patients with COPD. However, the mechanisms

Mathematical modeling represents and predicts biological systems, explains underlying mechanisms, constituting one of the key focus points for fundamental and applied research to improve our understanding and to decrease costs. Organic acids are used in several industries such as monomers for bioplastics, food preservatives and additives, pharmaceuticals, agriculture. Non-petrochemical, sustainable