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Correlation between plasma PSGL-1 and FIGO stage, tumor metastasis, and survival in epithelial ovarian cancer Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-17 WenHui Li, Cheng Fang, Ya Gao, Yan Gao, FengShang Yan, BiLiang Chen, MingJuan Xu
Plasma circulating P-selectin glycoprotein ligand-1 (PSGL-1) levels and its clinical correlation in patients with epithelial ovarian cancer (EOC) are unknown. The study determined plasma PSGL-1 levels in EOC patients and investigated its relationship with clinicopathological factors and prognosis. Plasma PSGL-1 levels were measured using ELISA in 69 patients with EOC, 34 patients with benign ovarian
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Optimization of hematite nanoparticles from natural ore as novel imaging agents: A Green Chemistry approach Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-15 Albert Asha, Munusamy Chamundeeswari, Rayappan Mary Nancy Flora, Kuppusamy Ravichandran Preethy
In this research, we propose an environmentally friendly method for producing hematite nanoparticles (H‐NPs) from natural hematite ore, focusing on their application as efficient contrast agents in x‐ray and computed tomography (CT) imaging for medical purposes. The process involves the reduction of iron oxide within the ore to attain the desired hematite phase, crucial for synthesizing H‐NPs. To ensure
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Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-14 Parastoo Pourali, Milan Svoboda, Eva Neuhöferová, Volha Dzmitruk, Veronika Benson
The biologically produced gold nanoparticles (AuNPs) are novel carriers with promising use in targeted tumor therapy. Still, there are no studies regarding the efficacy of nanoparticle internalization by cancer and noncancer cells. In this study, AuNPs were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), energy dispersive x‐ray spectroscopy
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Development of a prognostic model for lung adenocarcinoma polarity‐related genes and analysis of immune landscape Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-13 Hongqiu Xu, Wenqiang Du, Xuelong Jing, Jingen Xie, Pengfei Li
Despite the progress made in the management of lung adenocarcinoma (LUAD), the overall prognosis for LUAD individuals remains suboptimal. While the role of cell polarity in tumor invasion and metastasis is well established, its prognostic significance in LUAD is still unknown. Differential analysis was performed on the Cancer Genome Atlas (TCGA)‐LUAD and normal lung tissue, and candidate genes were
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Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-13 Baby Shakila P, Tamilmani Periasamy, Abdullah A. Alarfaj, Palanisamy Arulselvan, Rajeswari Ravindran, Jagadeesh Suriyaprakash, Indumathi Thangavelu
Supramolecular nanoparticles containing peptides and drugs have recently gained recognition as an effective tumor treatment drug delivery system. A multitarget drug termed pemetrexed is effective against various cancers, including nonsmall cell lung cancer. The work aims to establish the capability of pemetrexed gold nanoparticles (PEM‐AuNPs) to induce apoptosis and explore molecular changes. X‐ray
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A novel graphene oxide‐based fluorescence method for detection of urine glycosaminoglycans Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-07 Ceren Bakır Kösoğlu, Süreyya Dede, Emine Karakuş, Ali Erdoğmuş, Bahadır Keskin, Hasan Önal
Glycosaminoglycans (GAGs) serve as a biomarker for mucopolysaccharidoses disease. In this study, a novel fluorometric method was developed to measure total GAGs in urine. Graphene oxide (GO) and rhodamine B (RhB), a cationic fluorescent dye, were employed in the development of the method. RhB attaches to the GO surface via electrostatic attraction, leading to the quenching of its fluorescence upon
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Metabolite profiling of Piper longum L. fruit volatiles by two‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-06 Manaswini Dash, Subhashree Singh, Suprava Sahoo, Malvika Dutt, Basudeba Kar
Piper longum L. (long pepper) is an economically and industrially important medicinal plant. However, the characterization of its volatiles has only been analyzed by gas chromatography–mass spectrometry (GC–MS). In the present study, precise characterization of P. longum fruit volatiles has been performed for the first time through advanced two‐dimensional gas chromatography–time‐of‐flight spectrometry
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Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-03-04 Guilherme Schwingel Henn, Larissa Zago Cima, Beatriz Fabris Bettanin, Cláudia Schlabitz, Daniel Neutzling Lehn, Claucia Fernanda Volken de Souza, Lucélia Hoehne
This study aimed to assess the technical feasibility of incorporating selenized Lactobacillus spp. microencapsulated via spray drying into cattle feed. Gum Arabic and maltodextrin were used as encapsulating agents. The encapsulation process was carried out with a drying air flow rate of 1.75 m3/min, inlet air temperature of 90°C, and outlet air temperature of 75°C. The viability of the encapsulated
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Bacoside‐A repressed the differentiation and lipid accumulation of 3T3‐L1 preadipocytes by modulating the expression of adipogenic genes Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-29 Thiyagarajan Ramesh, Mohammad Shahid
Obesity is one of the more complicated diseases, it can induce numerous life‐threatening diseases mainly diabetes mellitus, cardiovascular disease, hypertension, and certain cancers. In this study, we assessed the efficacy of bacoside‐A (a dammarane‐type triterpenoid saponin derived from the plant Bacopa monniera Linn.) on the adipogenesis of 3T3‐L1 preadipocytes. Results of this study illustrated
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Waste cooking oil and molasses for the sustainable production of extracellular lipase by Saitozyma flava Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-27 Silvia Donzella, Andrea Fumagalli, Martina Letizia Contente, Francesco Molinari, Concetta Compagno
Organic waste valorization is one of the principal goals of the circular economy. Bioprocesses offer a promising approach to achieve this goal by employing microorganisms to convert organic feedstocks into high value products through their metabolic activities. In this study, a fermentation process for yeast cultivation and extracellular lipase production was developed by utilizing food waste. Lipases
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Hsa_circ_0001480 affects osteosarcoma progression by regulating the miR‐363‐3p/IBSP pathway Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-27 Guijuan Bi, Li Zhang
Osteosarcoma (OS) is a malignant bone tumor that commonly affects young individuals. Circular RNAs (circRNAs) are associated with OS progression. In this study, we aimed to determine the role of hsa_circ_0001480 (circ_0001480) in OS development. OS cell invasion, viability, and colony numbers were assessed via transwell, cell counting kit‐8, and colony formation assays, respectively. Tumor growth in
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Analysis of human epidermal growth factor receptor 2 interaction on aptamer‐probed interdigitated electrode for breast cancer diagnosis Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-27 Yingxin Hao, Subash C. B. Gopinath
Breast cancer has been reported to be high in its incidence with women, and early identification of breast cancer helps to improve and provide an effective treatment. Tumor markers are active substances; in particular, human epidermal growth factor receptor 2 (HER2) is over‐expressed at the level of 20%–30%. This research work developed a highly sensitive HER2 biosensor on the interdigitated electrode
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Skin wound healing in diabetic rat model using low‐dose photodynamic therapy Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-27 Khatereh Khorsandi, Reza Fekrazad
Chronic wound is one of the major challenges in medicine and imposes a heavy financial burden on the healthcare of different countries. Diabetic foot ulcers as one of the important examples for chronic wounds can lead to lower limb amputation, disability, and death in diabetics. In this regard, novel technology with low side effects got attention in recent years. Low‐dose photodynamic therapy (LDPDT)
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Neuroprotective effects of annexin A1 tripeptide in rats with sepsis‐associated encephalopathy Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-27 Qiao Cui, Nannan Qin, Yonghan Zhang, Yanmei Miao, Leiyu Xie, Xinglong Ma, Zhiquan Zhang, Peng Xie
Sepsis‐associated encephalopathy (SAE) is characterized by high incidence and mortality rates, with limited treatment options available. The underlying mechanisms and pathogenesis of SAE remain unclear. Annexin A1 (ANXA1), a membrane‐associated protein, is involved in various in vivo pathophysiological processes. This study aimed to explore the neuroprotective effects and mechanisms of a novel bioactive
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Nanoliposome-loaded phenolics from Salvia leriifolia Benth and its anticancer effects against induced colorectal cancer in mice Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-07 Niusha Shojaee Nasrabadi, Arezoo Vedad, Kimia Asadi, Mahsa Rastegar Moghaddam Poorbagher, Nadia Abdollahian Tabrizi, Kiyana Dorooki, Roghayeh Sabouhi Sabouni, Mahla barati moghadam, Negin Shafaei, Ehsan Karimi, Ehsan Oskoueian
Colon cancer is one of the leading causes of death among various types of cancer. Despite the significant progress made in cancer treatment, chemotherapy resistance and various side effects are still prevalent. The objective of this study is to assess the therapeutic potential of phenolic-rich fraction encapsulated nanoliposome (PRF-NLs) of Salvia leriifolia Benth in the treatment of colon cancer in
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Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-04 Asad Ullah, Xian Yin, Muhammad Naveed, Sadar Aslam, Malik Wajid Hussain Chan, Sun Bo, Fenghuan Wang, Bo Xu, Baocai Xu, Zhou Yu
In this study, the transcriptome analysis was practiced to identify potential genes of probiotic Bacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation of the strain was deliberated in presence of three different sodium selenite concentrations (0, 3, and 20 μg/mL). The samples were taken at 1 and 13 h subsequent to inoculation of selenite and gene expression
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HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC-MS/MS peptide mass fingerprinting Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-04 Dwarakanath P. Radhakrishnan, Abinaya Kanakaraja, Nagasathiya Krishnan, Meenakumari Sakthivelu, Subash C. B. Gopinath, Raman Pachaiappan
Parmotrema perlatum, a lichen belonging to the family Parmeliaceae, is well known for its culinary benefits and aroma used as a condiment in Indian homes is also known as the “black stone flower” or “kalpasi” in India. This research intends to analyze the antioxidant power of the crude extracts using four pH-based buffers solubilized proteins/peptides and RP-HPLC fractions of P. perlatum obtained by
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Optimization and chemical free fabrication of green synthesized iron nanoparticles as potential MRI contrast agent Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-02-01 Yuwvaranni S, Punitha N, Chamundeeswari M
The current research article has investigated the synthesis and characterization of novel iron nanoparticles (INPs) from neem and betel leaves extract combination using response surface methodology–central composite design and coated with chitosan–curcumin (CCINPs) as a biocompatible and contrast agent for magnetic resonance imaging (MRI). The coating of INPs with chitosan and curcumin (CCINPs) was
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Improving the substrate binding of acetyl-CoA carboxylase (AccB) from Streptomyces antibioticus through computational enzyme engineering Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-29 Imtiaz Ali, Dong-Qing Wei, Abbas Khan, Yuanyuan Feng, Muhammad Waseem, Zahid Hussain, Arshad Iqbal, Syed Shujait Ali, Anwar Mohammad, Jianting Zheng
Malonyl-CoA serves as the main building block for the biosynthesis of many important polyketides, as well as fatty acid–derived compounds, such as biofuel. Escherichia coli, Corynebacterium gultamicum, and Saccharomyces cerevisiae have recently been engineered for the biosynthesis of such compounds. However, the developed processes and strains often have insufficient productivity. In the current study
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Analysis of genes specific to the early maturation stage of Sesamum indicum seeds by subtraction method*,** Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-28 Yu Okuno, Akihiro Kishikawa, Hisashi Imakouji, Motonobu Yoshida
The mechanisms regulating the content ratio of unsaturated fatty acid in sesame oil need to be clarified in order to breed novel varieties with high contents of unsaturated fatty acids. Full-length cDNA libraries prepared from sesame seeds 1 to 3 weeks after flowering were subtracted with cDNAs from plantlets of 4 weeks after germination. A total of 1545 cDNA clones was sequenced. The functions of
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Spontaneous nanoemulsification of cinnamon essential oil: Formulation, characterization, and antibacterial and antibiofilm activity against fish spoilage caused by Serratia rubidaea BFMO8 Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-22 Jasmin Suriya Arul Raj, Sheena Aliyas, Yolin Angel Poomany Arul Soundara Rajan, Kasi Murugan, Ponmurugan Karuppiah, Natarajan Arumugam, Abdulrahman I. Almansour, Perumal Karthikeyan
The contemporary food industry's uses of nanoemulsions (NEs) include food processing, effective nutraceutical delivery, the development of functional chemicals, and the synthesis of natural preservatives, such as phytocompounds. Although cinnamon essential oil (CEO) is widely used in the cosmetic, pharmaceutical, and food industries, it is difficult to add to aqueous-based food formulations due to
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Intramyocardial delivery of injectable hydrogel with arctigenin alleviated myocardial ischemia–reperfusion injury in rats Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-21 Chengyin Liu, Xuejun Jiang, Lanyu Liang, Han Liu, Li Li, Qing Shan
Arctigenin belongs to a major bioactive component of Fructus arctii and has been found with cardioprotective effects on rats with ischemia‒reperfusion (I/R) injury. The application of arctigenin is limited due to poor water solubility and low bioavailability. Hydrogel drug delivery systems can improve the efficacy and safety of drugs, increase drug utilization, and reduce side effects. We hypothesized
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Poly-γ-glutamic acid overproduction of Bacillus licheniformis ATCC 9945a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-21 Mehrdad Ebrahimzadeh Kouchesfahani, Ali Bahrami, Valiollah Babaeipour
The commercial production of multifunctional, biocompatible, and biodegradable biopolymers such as poly-γ-glutamic acid via microbial fermentation requires the development of simple and cheap methods for mass production. This study optimized the poly-γ-glutamic acid production of Bacillus licheniformis ATCC 9945a in several steps. At first, the most critical components of the culture medium, including
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The importance, benefits, and future of nanobiosensors for infectious diseases Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-18 Th. S. Dhahi, Alaa Kamal Yousif Dafhalla, Sawsan Ali Saad, Duria Mohammed Ibrahim Zayan, Amira Elsir Tayfour Ahmed, Mohamed Elshaikh Elobaid, Tijjani Adam, Subash C. B. Gopinath
Infectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates and controlling disease spread. Traditional laboratory-based diagnostic methods face challenges such as high costs, time consumption, and a lack of trained personnel in resource-poor settings. Diagnostic biosensors
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Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-17 Sirwan Khanchezar, Valiolah Babaeipour, Atiyeh Sadat Mostafa
In this study, a cost-effective complex culture media containing molasses and corn steep liquor (CSL) was developed for the high production of bacterial cellulose (BC) by investigating the effect of four effective factors on BC production at three levels using Taguchi and combined methods. The predicted and actual values of BC production in optimal conditions by Taguchi and combined methods were 8
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Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-15 Shadan Raza, Renu Poria, Deepak Kala, Nishant Sharma, Anil K. Sharma, Nkurunziza Florien, Hardeep S. Tuli, Ankur Kaushal, Shagun Gupta
Globally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100–300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild
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Construction and characterization of a promoter library with varying strengths to enhance acetoin production from xylose in Serratia marcescens Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-15 Jie Liu, Di Liu, Tingting Sun, Tai-Ping Fan, Yujie Cai
Serratia marcescens is utilized as a significant enterobacteria in the production of various high-value secondary metabolites. Acetoin serves as a crucial foundational compound of development and finds application in a broad range of fields. Furthermore, S. marcescens HBQA-7 is capable of utilizing xylose as its exclusive carbon source for acetoin production. The objective of this study was to utilize
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Biotechnological insights into extracellular enzyme production by thermotolerant fungi from hot springs and caves: Morphology, pellets formation, and protease production Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-15 Adriana Jazmín Legorreta-Castañeda, Guadalupe Guerra-Sánchez, Karina García-Gutiérrez, Dario Rafael Olicón-Hernández
This study investigates the thermotolerant fungal biodiversity in caves and hot springs, focusing on their potential for extracellular enzyme production, specifically proteases. Samples were collected from the Cardonal region in Hidalgo, Mexico, using three different isolation methods. The study characterizes the morphological diversity of the isolated fungi and identifies various genera, including
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Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496 Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-15 Qian Tan, Linbo Gou, Tai-Ping Fan, Yujie Cai
Clostridium aceticum DSM1496 is an acid-resistant strain in which ornithine decarboxylase (ODC) plays a crucial role in acid resistance. In this study, we expressed ODC derived from C. aceticum DSM1496 in Escherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27 kDa and uses pyridoxal-5′-phosphate (PLP) as a coenzyme with a Km = 0.31 mM
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Production of the prolyl endoprotease (PEP) from Aspergillus sp. FSDE 16 by solid-state fermentation (SSF) and use for producing a gluten-free beer Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-11 Thaís Cartaxo de Almeida, Sharline Florentino de Melo Santos, Everaldo Silvino dos Santos
Beer is a beverage that contains gluten and cannot be consumed by people with celiac disease. In this context, the enzyme prolyl endoprotease (PEP) can be used to reduce the gluten content in beer. The present study aimed to produce the PEP from Aspergillus sp. FSDE 16 using solid-state fermentation with 5 conditions and comparing with a similar commercial enzyme produced from Aspergillus niger in
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In vitro and in vivo assessment of antimicrobial, enzymatic, and antifouling properties of self-potent lichen symbiotic bacteria Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2024-01-07 Rubavathi Subbaiyan, Ayyappadasan Ganesan
This study aimed to comprehensively assess the antimicrobial, antifouling, and antibiofilm-forming potential of lichen symbiotic bacteria against marine fouling bacterial strains. A total of 50 lichen-associated bacteria (LAB) isolates were successfully characterized and evaluated for their effectiveness in mitigating biofouling caused by various marine biofoulers. Through a battery of biological assays
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Extraction process, physicochemical properties, and digestive performance of red yeast rice starch Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-21 Mengting Bai, Kai Liu, Yulin Wang, Shuguang Hou, Xiaofang Li, Jia Luo
In the present study, taking red yeast rice (RYR) as the raw material, the optimum extraction process of RYR starch was investigated through a single-factor experiment and the Box–Behnken design: The liquid–to-solid ratio was 5 mL/g, the concentration of sodium hydroxide solution was 0.075 mol/L, and the extraction time was 3.1 h. Under these extraction conditions, the extraction rate of starch reached
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Mucilage-assisted fabrication of molybdenum trioxide nanostructures for photothermal ablation of breast cancer cells Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-19 Reem A. Alomari, Jegan Athinarayanan, Vaiyapuri Subbarayan Periasamy, Ali A. Alshatwi
Nanostructures have been used for various biomedical applications due to their optical, antibacterial, magnetic, antioxidant, and biocompatible properties. Cancer is a prevalent disease that severely threatens human life and health. Thus, innovative and effective therapeutic approaches are urgently required for cancer. Photothermal therapy (PTT) is a promising approach to killing cancer cells. In this
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Improving the capturing ability of swirl-based microfluidic chip by introducing baffle wall Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-11 Yanping Dang, Qin Zhang, Zhiming Ou, Shuai Hu
Microfluidics technology is promising in developing microparticle manipulation technology due to its nondestructive control and notable adaptability. The manipulation of microparticle based on swirling stagnation point is one of the feasible microfluidics biotechnologies. Aiming to improve the regulation and control of microparticle, baffle wall is introduced into the 2-microchannel flow field. The
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Resveratrol-loaded decellularized ovine pericardium: ECM introduced for tissue engineering Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-11 Mozafar Khazaei, Morteza Alizadeh, Leila Rezakhani
An ideal scaffold for skin tissue engineering should have a suitable potential for antibacterial activity, no hemolysis, sufficient porosity for air exchange, water retention capacity, and a suitable swelling rate to maintain tissue moisture. Considering this issue, our study used decellularized ovine pericardial tissue's extracellular matrix (ECM). These scaffolds were decellularized with sodium dodecyl
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Inhibition of biofilm, quorum-sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-07 Yasemin İspirli Doğaç, Alfred Ngenge Tamfu, Selahattin Bozkurt, Mehmet Kayhan, Mustafa Teke, Ozgur Ceylan
Resistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe3O4 np), manganese ferrite (MnFe2O4 np) and nickel ferrite (NiFe3O4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red
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Evaluation of the relationship of cytokines concentrations tumor necrosis factor-alpha, interleukin-6, and C-reactive protein in obese diabetics and obese non-diabetics: A comparative study Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-06 Imad Jassim Mohammad, Soheila Kashanian, Ronak Rafipour, Husam Aljwaid, Sadegh Hashemi
Obesity has been linked to a low-grade inflammatory process in the white adipose tissue. Our study aims to detect the relationship between cytokine levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP) in obese diabetics, compared to obese non-diabetics, Iraqi individuals. Ninety Iraqi adults, 45 type 2 diabetic and 45 non-diabetic obese, were selected as
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Analysis of highly frequent point mutations in glycoprotein C, glycoprotein N, and nucleoprotein of CCHFV Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-06 Neha Kaushal, Manoj Baranwal
Crimean-Congo hemorrhagic fever virus (CCHFV) is classified among top 10 priority pathogens by World Health Organization. CCHFV belongs to Bunyaviridae family and negative sense ssRNA genome composed of three RNA segments: L, M, and S. RNA viruses show higher mutation rate as compared to DNA viruses. To gain deeper understanding of impact of point mutations in CCHFV M and S segment, mutation profiling
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High-sensitivity immunoassay on interdigitated electrode to detect osteoporosis biological marker Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-12-06 Jianwen Liu, Xiaohai Chen, Hanlin Lv, Shuming He, Yong Fan
Osteoporosis is with porous bones, which refers to a decrease in the bone mineral density and weakens the bones to become brittle. Osteoporosis often progresses without any pain or symptoms until the bone fractures. Monitoring the condition of bone regularly helps to identify the bone that weakens at its earlier stages. In general, radiological techniques have been used to measure bone mineral density
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Tetrahydropalmatine from medicinal plants activates human glucokinase to regulate glucose homeostasis Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-30 Sweta Singh, Payel Ghosh, Shilpy Sharma, Shobha Bhargava, Ameeta Ravi Kumar
Many synthetic glucokinase activators (GKAs), modulating glucokinase (GK), an important therapeutic target in diabetes have failed to clear clinical trials. In this study, an in silico structural similarity search with differing scaffolds of reference GKAs have been used to identify derivatives from natural product databases. Ten molecules with good binding score and similar interactions to that in
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Immunogenicity of different nanoparticle adjuvants containing recombinant RBD coronavirus antigen in animal model Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-30 Fatemeh Ataei, Ali Ahmadi, Mahdi Fasihi-R, Reza Kachoei, Jafar Amani, Ali Najafi
Ongoing mutations of SARS-CoV-2 present challenges for vaccine development, promising renewed global efforts to create more effective vaccines against coronavirus disease (COVID-19). One approach is to target highly immunogenic viral proteins, such as the spike receptor binding domain (RBD), which can stimulate the production of potent neutralizing antibodies. This study aimed to design and test a
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Cationic ribosomal proteins can inhibit pro-inflammatory action stimulated by LPS+HMGB1 and are hindered by advanced glycation end products Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-27 Masahiro Watanabe, Takao Toyomura, Hidenori Wake, Takashi Nishinaka, Omer Faruk Hatipoglu, Hideo Takahashi, Masahiro Nishibori, Shuji Mori
We previously found that ribosomal protein L9 (RPL9) is a novel advanced glycation end product (AGE)-binding protein that can decrease pro-inflammatory TNF-α expression stimulated by lipopolysaccharide (LPS) plus high-mobility group box 1 (HMGB1), suggesting that RPL9 has a role in regulating LPS+HMGB1-stimulated inflammatory reactions. Among the various ribosomal proteins, it was found that RPS5 reproduced
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FTO-mediated ZNF687 accelerates tumor growth, metastasis, and angiogenesis in colorectal cancer through the Wnt/β-catenin pathway. Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-20 Junyi Li,Shixin Li,Xiaoxiao Xing,Nini Liu,Suyu Lai,Daixiang Liao,Jun Li
Colorectal cancer (CRC) is a common and lethal cancer. ZNF687 has been disclosed to take part in diversified cancers' progression by serving as a facilitator. However, the detailed regulatory functions of ZNF687 in the CRC have not been investigated. This work is planned to probe the impacts of ZNF687 on CRC progression. The IHC, RT-qPCR, and western blot assays were used to examine mRNA and protein
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Sulfonamide derivatives with benzothiazole scaffold: Synthesis and carbonic anhydrase I–II inhibition properties Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-14 Cansu Öztürk, Erbay Kalay, Serpil Gerni, Neslihan Balci, Feyzi Sinan Tokali, Osman Nuri Aslan, Emrah Polat
The secondary sulfonamide derivatives containing benzothiazole scaffold (1–10) were synthesized to determine their inhibition properties on two physiologically essential human carbonic anhydrases isoforms (hCAs, EC, 4.2.1.1), hCA I, and hCA II. The inhibitory effects of the compounds on hCA I and hCA II isoenzymes were investigated by comparing their IC50 and Ki values. The Ki values of compounds (1–10)
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Inhibition of USP21 leads to ovarian carcinoma cell death by suppressing MAPK signaling Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-14 Xin Sun, Jia Yu, Xiaorong Cui, Yujie Tang, Yani Yu
Ovarian cancer is the most aggressive and lethal of all gynecologic malignancies. Although the overexpression (OE) of ubiquitin-specific peptidase 21 (USP21) has been observed in multiple cancers, its expression profile and biological function in ovarian cancer remain unknown. The expression levels of USP21 in ovarian cancer cells and tissues as well as adjacent normal tissues were assessed by qRT-PCR
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Sequence identification and in silico characterization of novel thermophilic lipases from Geobacillus species Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-11-01 Yusuf Sürmeli, Hasan Cihad Tekedar, Gülşah Şanlı-Mohamed
Microbial lipases are utilized in various biotechnological areas, including pharmaceuticals, food, biodiesel, and detergents. In this study, we cloned and sequenced Lip21 and Lip33 genes from Geobacillus sp. GS21 and Geobacillus sp. GS33, then we in silico and experimentally analyzed the encoded lipases. For this purpose, Lip21 and Lip33 were cloned, sequenced, and their amino acid sequences were investigated
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Homology modeling, virtual screening, molecular docking, and ADME approaches to identify a potent agent targeting NK2R protein Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-30 Arash Soltani, Seyed Isaac Hashemy
Neurokinin/tachykinin receptors are classified as the G-protein coupled receptor superfamily. The neurokinin 2 receptor (NK2R) is widely expressed in different tissues. NK2R is associated with a range of biological events, such as inflammation, smooth muscle contraction, intestinal motor functions, and asthma. Despite these diverse activities, no approved drugs targeting NK2R have been developed yet
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A two-step electrochemical biosensor based on Tetrazyme for the detection of fibrin Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-30 Jiayi Jiang, Bin Wang, Linghuan Luo, Na Ying, Gaofan Shi, Mengmeng Zhang, Haoyuan Su, Dongdong Zeng
In this study, an electrochemical biosensor was constructed for the detection of fibrin, specifically by a simple two-step approach, with a novel artificial enzyme (Tetrazyme) based on the DNA tetrahedral framework as signal probe. The multichannel screen-printed electrode with the activated surface cannot only remove some biological impurities, but also serve as a carrier to immobilize a large number
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Development of a new affinity chromatography method for purification of horseradish peroxidase enzyme Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-30 Serpil Gerni, Hasan Özdemir
In this study, benzohydroxamic acid molecules were synthesized from methyl 4-amino-2-methoxy, methyl 4-amino-3-nitro, methyl 4-amino-3-methyl, and methyl 4-amino-3-chloro benzoate molecules, and the horseradish peroxidase (HRP) enzyme was purified in one step using the affinity chromatography technique for the first time. The IC50 and Ki values for the 4-amino 3-methyl benzohydroxamic acid molecule
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Protective effect of human epicardial adipose-derived stem cells on myocardial injury driven by poly-lactic acid nanopillar array Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-30 Wenju Yan, Yan Li, Jie Yin, Qian Liu, Yugen Shi, Jiayu Tan, Yu Wang, Shan Zhang, Junyi Zhang, Jingxin Li, Suhua Yan
We investigated if poly-lactic acid (PLA) nanopillar array can trigger the differentiation of human epicardial (ADSCs) (heADSCs) into cardiomyocyte-like cells and explored the effects of these cardiomyocyte-like cells on myocardial infarction (MI) in vivo. PLA nanopillar array (200 nm diameter) and plain PLA film (PLA planar) induced heADSCs were marked with carboxyfluorescein. After 7 days, the expressions
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Identification of angiogenesis-related subtypes, the development of a prognosis model, and features of tumor microenvironment in colon cancer Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-25 Feifei Wang, Changjing Wang, Baokun Li, Guanglin Wang, Zesong Meng, Jiachao Han, Ganlin Guo, Bin Yu, Guiying Wang
Angiogenesis is associated with tumor progression, prognosis, and treatment effect. However, the angiogenesis’ underlying mechanisms in the tumor microenvironment (TME) still remain unclear. Understanding the dynamic interactions between angiogenesis and TME in colon adenocarcinoma (COAD) is necessary. We downloaded the transcriptome data and corresponding clinical data of colon cancer patients from
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Purification and characterization of thioredoxin reductase enzyme from commercial Spirulina platensis tablets by affinity chromatography and investigation of the effects of some chemicals and drugs on enzyme activity Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-20 Eda Dagsuyu, Refiye Yanardag
Thioredoxin reductase (TrxR, enzyme code [E.C.] 1.6.4.5) is a widely distributed flavoenzyme that catalyzes nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of thioredoxin and many other physiologically important substrates. Spirulina platensis is a blue–green algae that is often used as a dietary supplement. S. platensis is rich in protein, lipid, polysaccharide, pigment, carotenoid
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The impact of oleuropein on miRNAs regulating cell death signaling pathway in human cervical cancer cells Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-17 Zeinab Amini-Farsani, Somayeh Hashemi Sheikhshabani, Nasibeh Shaygan, Samira Asgharzade
Cervical cancer is known as the second most pervasive malignancy in women across the globe. The role played by microRNAs (miRNAs) in the initiation, progression, and metastasis of this cancer has received specific attention. The use of natural compounds leading cancer cells toward apoptosis is a feasible strategy for cancer therapy. Oleuropein, an olive-extracted phenolic substance, displays anticancer
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Neuroprotective effect of biogenically synthesized ZnO nanoparticles against oxidative stress and β-amyloid toxicity in transgenic Caenorhabditis elegans Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-17 Renuka Mani, Dhineshkumar Ezhumalai, Ganesan Muthusamy, Elangovan Namasivayam
Amyloid β (Aβ) plaque accumulation-mediated neuronal toxicity has been suggested to cause synaptic damage and consequent degeneration of brain cells in Alzheimer's disease (AD). With the increasing prerequisite of eco-friendly nanoparticles (NPs), research investigators are utilizing green approaches for the synthesis of zinc oxide (ZnO) NPs for pharmaceutical applications. In this present study, ZnO
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Development of a continuous fermentation process for the production of recombinant uricase enzyme by Pichia pastoris Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-16 Sanaz Mahboudi, Seyed Abbas Shojaosadati, Amir Maghsoudi, Behrouz Mahmoudi
Recent studies in the biopharmaceutical industry have shown an increase in the productivity and production efficiency of recombinant proteins by continuous culture. In this research, a new upstream fermentation process was developed for the production of recombinant uricase in the methylotrophic yeast Pichia pastoris. Expression of recombinant protein in this system is under the control of the AOX1
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Strategies on aroma formation in Chardonnay sparkling base wine: Different Saccharomyces cerevisiae strains, co-inoculation with Torulaspora delbrueckii and utilization of bentonite Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-16 Lilian Raquel Hickert, Andressa Cattani, Luciano Manfroi, Roger Wagner, Junior Mendes Furlan, Voltaire Sant'Anna
The worldwide production of sparkling wines has been growing annually, driven by a market demand for high quality and more complex products. The present study aimed to evaluate the fermentation of Chardonnay must using two different Saccharomyces cerevisiae yeasts, either alone (from commercial brands A and B) or in combination with Torulaspora delbrueckii (ScA + Td and ScB + Td, respectively), as
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Response surface methodology use in construction of polianiline-coated carbon paste electrode–based biosensor: Modification and characterization Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-16 Esra Yağız, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
In this study, the effect of amperometric glucose biosensor construction and using conditions on the current response was investigated in detail applying experimental design. Polyaniline (PANI) was synthesized on the carbon paste electrode (CPE) surface using the cyclic voltammetry technique in sodium oxalate (NaOx) electrolyte medium, and an amperometric biosensor was constructed by immobilizing glucose
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Fusion proteins development strategies and their role as cancer therapeutic agents Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-11 Shakira Aslam, Faisal Zulfiqar, Warda Hameed, Shahnila Qureshi, Zaroon, Hamid Bashir
Cancer continues to be leading cause of morbidity and mortality despite decades of research and advancement in chemotherapy. Most tumors can be reduced via standard oncology treatments, such as chemotherapy, radiotherapy, and surgical resection, and they frequently recur. Significant progress has been made since targeted cancer therapy inception in creation of medications that exhibit improved tumor-selective
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Evaluation of the effect of designed PLGA-arctiin nanoparticles modified with folic acid and chitosan on colon cancer cells Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-10 Mohammed Hussein Ajel Alhadidi, Masoud Homayouni Tabrizi, Maryam Ghobeh
In this study, we designed nanoparticles (NPs) based on polylactic acid glycolic acid modified with chitosan and folic acid to optimize the anti-cancer, anti-inflammatory, and antioxidant effects of arctiin (ARC), and we measured its effects on cancer cells, including colon cancer. NPs were synthesized using the W1/O/W2 double-emulsion solvent evaporation method. Physicochemical characteristics of
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Pharmamedia and pectinase production by Bacillus subtilis Mz-12P Biotechnol. Appl. Bioc. (IF 2.8) Pub Date : 2023-10-02 Muaaz Mutaz Alajlani
Bacterial isolates collected from the environment were screened for pectinolytic activity, and a strain with the highest activity was selected and identified as Bacillus subtilis Mz-12. The presence of pectin hydrolase, lyase, and esterase activities was confirmed. Pectinase was purified and characterized. Enzyme production was optimized with respect to temperature, pH, and growth medium. Enzyme stability