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Biological characteristics of Muse cells derived from MenSCs and their application in acute liver injury and intracerebral hemorrhage diseases Regen. Ther. (IF 4.3) Pub Date : 2024-03-10 Han Li, Jinghui Wei, Mingzhi Li, Yaoqiang Li, Tong Zhang, Jialu Tian, Xuejia Liu, Kangjia Li, Juntang Lin
The increasing interest in multilineage differentiating stress-enduring (Muse) cells within the field of regenerative medicine is attributed to their exceptional homing capabilities, prolonged viability in adverse conditions, and enhanced three-germ-layer differentiate ability, surpassing their parent mesenchymal stem cells. Given their abundant sources, non-invasive collection procedure, and periodic
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Determining the recurrence rate of premature ventricular complexes and idiopathic ventricular tachycardia after radiofrequency catheter ablation with the help of designing a machine-learning model Regen. Ther. (IF 4.3) Pub Date : 2024-03-10 Entezar Mehrabi Nasab, Saeed Sadeghian, Ali Vasheghani Farahani, Ahmad Yamini Sharif, Farzad Masoud Kabir, Houshang Bavanpour Karvane, Ahora Zahedi, Ali Bozorgi
Ventricular arrhythmias increase cardiovascular morbidity and mortality. Recurrent PVCs and IVT are generally considered benign in the absence of structural heart abnormalities. Artificial intelligence is a rapidly growing field. In recent years, medical professionals have shown great interest in the potential use of ML, an integral part of AI, in various disciplines, including diagnostic applications
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A passage-free, simplified, and scalable novel method for iPSC generation in three-dimensional culture Regen. Ther. (IF 4.3) Pub Date : 2024-03-10 Masaya Tsukamoto, Tomoyuki Kawasaki, Mohan C. Vemuri, Akihiro Umezawa, Hidenori Akutsu
Induced pluripotent stem cells (iPSCs) have immense potential for use in disease modeling, etiological studies, and drug discovery. However, the current workflow for iPSC generation and maintenance poses challenges particularly during the establishment phase when specialized skills are required. Although three-dimensional culture systems offer scalability for maintaining established iPSCs, the enzymatic
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Improving cardiac differentiation of human pluripotent stem cells by targeting ferroptosis Regen. Ther. (IF 4.3) Pub Date : 2024-03-08 Jeffrey Aalders, Laurens Léger, Behrouz Hassannia, Vera Goossens, Tom Vanden Berghe, Jolanda van Hengel
Generation of cardiomyocytes from human pluripotent stem cells (hPSCs) is of high interest for disease modelling and regenerative medicine. hPSCs can provide an unlimited source of patient-specific cardiomyocytes that are otherwise difficult to obtain from individuals. Moreover, the low proliferation rate of adult cardiomyocytes and low viability limits the quantity of study material. Most protocols
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Clustering human dental pulp fibroblasts spontaneously activate NLRP3 and AIM2 inflammasomes and induce IL-1β secretion Regen. Ther. (IF 4.3) Pub Date : 2024-03-07 Shafei Zhai, Lihui Zhang, Xue Li, Qi Yu, Changkui Liu
The objective of the present study was to investigate whether NOD-like receptor family pyrin domain-containing 3 (NLRP3) and absent in melanoma 2 (AIM2) inflammasomes pathways were involved in an experimental model of fibroblast activation named nemosis, which was used to mimic circumstances without bacteria stimulation. Nemosis of human dental pulp fibroblast (DPFs) was induced by three-dimensional
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Human umbilical cord mesenchymal stem cells protect MC3T3-E1 osteoblasts from dexamethasone-induced apoptosis via induction of the Nrf2-ARE signaling pathway Regen. Ther. (IF 4.3) Pub Date : 2024-03-05 Chen Qiu, Zhaowen Li, Puji Peng
To investigate the protective effect human umbilical cord mesenchymal stem cells (hUC-MSCs) have on Dexamethasone (Dex)-induced apoptosis in osteogenesis via the Nrf2-ARE signaling pathway. Glucocorticoid-induced osteonecrosis of the femoral head (GC-ONFH) was developed in rats through the administration of lipopolysaccharide and methylprednisolone. The incidence of femoral head necrosis, cavity notch
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Effect of basic fibroblast growth factor with collagen/gelatin fixture in a rabbit model of nasal septum perforation Regen. Ther. (IF 4.3) Pub Date : 2024-02-23 Yuki Takizawa, Tsunetaro Morino, Ryo Takagi, Nobuyoshi Otori, Hiromi Kojima, Masayuki Yamato
The treatment of nasal septum perforation solely by surgical intervention presents significant challenges. This study evaluated the effect of basic fibroblast growth factor (bFGF) in combination with collagen/gelatin on wound healing of nasal septum perforation in a rabbit animal model. A nasal septum perforation rabbit model was created. bFGF was added to a collagen/gelatin fixture and placed adjacent
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Bibliometric and visualized analysis of hydrogels in organoids research Regen. Ther. (IF 4.3) Pub Date : 2024-02-23 Jia-bo Wang, Jie Wu, Jian Zhang, Li-an Guan, Hai-bo Feng, Ke-yan Zhu, Yu Zhang, Wen-jie Zhao, Qing Peng, Bo Meng, Sheng Yang, Hua Sun, Ye-dong Cheng, Liang Zhang
Over the past decades, there has been ongoing effort to develop complex biomimetic tissue engineering strategies for in vitro cultivation and maintenance of organoids. The defined hydrogels can create organoid models for various organs by changing their properties and various active molecules. An increasing number of researches has been done on the application of hydrogels in organoids, and a large
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Therapeutic potential of mesenchymal stem cells for cerebral small vessel disease Regen. Ther. (IF 4.3) Pub Date : 2024-02-22 Dong-hua Chen, Jia-rong Huang, Shuo-lei Su, Qiong Chen, Bing-yi Wu
Cerebral small vessel disease (CSVD), as the most common, chronic and progressive vascular disease on the brain, is a serious neurological disease, whose pathogenesis remains unclear. The disease is a leading cause of stroke and vascular cognitive impairment and dementia, and contributes to about 20% of strokes, including 25% of ischemic strokes and 45% of dementias. Undoubtedly, the high incidence
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Bulk RNA sequencing reveals the comprehensive genetic characteristics of human cord blood-derived natural killer cells Regen. Ther. (IF 4.3) Pub Date : 2024-02-20 Takayuki Morimoto, Tsutomu Nakazawa, Ryosuke Maeoka, Ryosuke Matsuda, Mitsutoshi Nakamura, Fumihiko Nishimura, Shuichi Yamada, Ichiro Nakagawa, Young-Soo Park, Takahiro Tsujimura
Innate immune cells are important in tumor immunotherapy. Natural killer cells (NKCs) are also categorized as innate immune cells and can control tumor growth and metastatic spread. Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. NKC-based immunotherapy is a promising treatment strategy against GBM. We previously reported a feeder-free expansion system that yielded
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Exosomes from preconditioned mesenchymal stem cells: Tissue repair and regeneration Regen. Ther. (IF 4.3) Pub Date : 2024-02-14 Ruili Long, Shuai Wang
As a prominent research area in tissue repair and regeneration, mesenchymal stem cells (MSCs) have garnered substantial attention for their potential in the treatment of various diseases. It is now widely recognized that the therapeutic effects of MSCs primarily occur through paracrine mechanisms. Among these mechanisms, exosomes play a crucial role by exerting a series of regulatory effects on surrounding
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Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis Regen. Ther. (IF 4.3) Pub Date : 2024-02-09 Xiaofeng Chen, Dongling Cai, Hao Li, Qipeng Wei, Xi Li, Zhuangxun Han, Jinjun Liang, Junxian Xie, Jiajian Ruan, Jincheng Liu, Zhen Xiang, Wenxuan Dong, Weijun Guo
Intervertebral disc degeneration (IDD) is one of the major leading causes of back pain affecting the patient's quality of life. However, the roles of circular RNA (circRNA) in IDD remains unclear. This study aimed to explore the function and underlying mechanism of circ_0036763 in IDD. In this study, expressions of circ_0036763, U2 small nuclear RNA auxiliary factor 2 (U2AF2), miR-583 and aggrecan
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High pressure pasteurization: Simultaneous native tissue decellularization and sterilization Regen. Ther. (IF 4.3) Pub Date : 2024-02-08 Akihisa Otaka, Takashi Yamamoto, Tetsuji Yamaoka
Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated. This study investigated the disinfection efficacy of the HHP process at physiologically relevant 36 °C against difficult-to-kill
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Preface to the special issue of “Regenerative therapy”: “Future of regenerative medicine viewed by young scientists” Regen. Ther. (IF 4.3) Pub Date : 2024-02-08 Satoshi Hosoya, Kotaro Mimori
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Effect of acidosis on adipose-derived stem cell impairment and gene expression Regen. Ther. (IF 4.3) Pub Date : 2024-02-05 Kun Huang, Qinqin Wang, Huilong Qu, Xinyu Hu, Wenhao Niu, Anna Hultgårdh-Nilsson, Jan Nilsson, Chun Liang, Yihong Chen
Based on disappointing results of stem cell-based application in clinical trials for patients with critical limb ischemia, we hypothesized that the acidic environment might be the key factor limiting cell survival and function. In the present study, we used microdialysis to determine presence of acidosis and metabolic imbalance in critical ischemia. Moreover, we explored the effect of extracellular
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Extracellular vesicles derived from HuMSCs alleviate daunorubicin-induced cardiac microvascular injury via miR-186-5p/PARP9/STAT1 signal pathway Regen. Ther. (IF 4.3) Pub Date : 2024-02-03 Shule Zhang, Dong Li, Linghong Liu, Qing Shi, Xiuli Ju
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Fabrication of novel nanofiber composed of gelatin/alginate with zirconium oxide NPs regulate orthodontic progression of cartilage degeneration on Wnt/β-catenin signaling axis in MC3T3-E1 cells Regen. Ther. (IF 4.3) Pub Date : 2024-02-02 Hua Zhao
Natural macromolecules like alginate and gelatin are employed to create medication delivery systems that are both safe and effective. Zirconium nanoparticles (ZrO NPs) have been proposed as a means of enhancing the alginate-gelatin hydrogel's physical and biological properties. This study combines the synthesis of the biopolymers gelatin and alginate nanofibers with nanoparticles of zirconium oxide
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Minimally invasive scar release by autologous adipose tissue transfer for post-traumatic neuropathic pain Regen. Ther. (IF 4.3) Pub Date : 2024-02-01 Giorgio Pietramaggiori, Federico Ricci, Stefano L'Erario, Franco Bassetto, Saja Scherer
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circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation Regen. Ther. (IF 4.3) Pub Date : 2024-01-25 Dazhou Wu, Shengyi Chen, Dongdong Huang, Zhipeng Huang, Na Zhen, Zhenxu Zhou, Jicai Chen
This study aims to explore the mechanism of circ- AMOT-like protein 1 (Amotl1) in extracellular vesicles (Evs) derived from adipose-derived stromal cells (ADSCs) regulating SPARC translation in wound healing process. The morphology, wound healing rate of the wounds and Ki67 positive rate in mouse wound healing models were assessed by H&E staining and immunohistochemistry (IHC). The binding of IGF2BP2
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Effects of plasma rich in growth factors on wound healing in patients with venous ulcers Regen. Ther. (IF 4.3) Pub Date : 2024-01-25 Joyce B.A. Castro, Beatriz G.R. B. Oliveira, Gutemberg G. Alves, Alexis Trott, Lucien J. Houneau, Hye Chung Kang, Márcia A. Ferreira
Significant evidence suggests that plasma-rich in growth factors (PRGF) favor the repair of chronic wounds, enabling a rapid return to functionality. However, components of PRGF and their effects on persistent ulcers and epithelial tissues are not well characterized. The goals of this research were to analyze the biological properties of platelet-derived factors, to examine their effectiveness on healing
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Dynamic tracking of human umbilical cord mesenchymal stem cells (hUC-MSCs) following intravenous administration in mice model Regen. Ther. (IF 4.3) Pub Date : 2024-01-24 Sze-Piaw Chin, Marini Marzuki, Lihui Tai, Nurul Ashikin Mohamed Shahrehan, Christine Ricky, Audrey Fanty, Annas Salleh, Chui Thean Low, Kong-Yong Then, Susan Ling Ling Hoe, Soon Keng Cheong
In the past decades, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have sparked interest in cellular therapy due to their immunomodulatory properties. Nevertheless, the fate of hUC-MSCs in the body remains poorly understood. This study aimed to investigate the biodistribution, homing and clearance of systemically administered hUC-MSCs in healthy BALB/c mice model. hUC-MSCs were labelled
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Operator-derived particles and falling bacteria in biosafety cabinets Regen. Ther. (IF 4.3) Pub Date : 2024-01-19 Mitsuru Mizuno, Koki Abe, Takashi Kakimoto, Hisashi Hasebe, Naoki Kagi, Ichiro Sekiya
To ensure the sterility of cell products that cannot undergo conventional sterilization processes, it is imperative to establish and maintain a clean room environment, regulated through environmental monitoring, including particle counts. Nevertheless, the impact of particles generated by operators as potential contaminants remains uncertain. Thus, in this study, we conducted an accelerated test to
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Estradiol enhanced neuronal plasticity and ameliorated astrogliosis in human iPSC-derived neural models Regen. Ther. (IF 4.3) Pub Date : 2024-01-12 Sopak Supakul, Chisato Oyama, Yuki Hatakeyama, Sumihiro Maeda, Hideyuki Okano
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Investigation of the effectiveness of gelatin hydrolysate in human iPS-RPE cell suspension transplantation Regen. Ther. (IF 4.3) Pub Date : 2024-01-11 Shohei Kitahata, Michiko Mandai, Hinako Ichikawa, Yuji Tanaka, Toshika Senba, Keisuke Kajita, Sunao Sugita, Kazuaki Kadonosono, Masayo Takahashi
The retinal pigment epithelium (RPE) plays essential roles in maintaining retinal functions as well as choroidal capillaries and can lead to visual disorders if dysfunctional. Transplantation of human-induced pluripotent stem cell-derived RPE (hiPSC-RPE) is a promising therapy for such RPE impaired conditions including age-related macular degeneration. The challenge with cell suspension transplantation
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Host-to-graft propagation of inoculated α-synuclein into transplanted human induced pluripotent stem cell-derived midbrain dopaminergic neurons Regen. Ther. (IF 4.3) Pub Date : 2024-01-06 Serina Gima, Kazuya Oe, Kaneyasu Nishimura, Takashi Ohgita, Haruka Ito, Hiroyuki Kimura, Hiroyuki Saito, Kazuyuki Takata
Cell therapeutic clinical trials using fetal mesencephalic tissue provided a proof-of-concept for regenerative therapy in patients with Parkinson's disease. Postmortem studies of patients with fetal grafts revealed that α-synuclein Lewy body (LB)-like inclusions emerged in long-term transplantation and might worsen clinical outcomes even if the grafts survived and innervated in the recipients. Various
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Evaluation of adipogenesis over time using a novel bioabsorbable implant without the addition of exogenous cells or growth factors Regen. Ther. (IF 4.3) Pub Date : 2024-01-04 Sunghee Lee, Shuichi Ogino, Yoshihiro Sowa, Kenta Yamamoto, Yuki Kato, Maria Chiara Munisso, Susumu Saito, Manabu Shirai, Tetsuji Yamaoka, Naoki Morimoto
Breast reconstruction is crucial for patients who have undergone mastectomy for breast cancer. Our bioabsorbable implants comprising an outer poly--lactic acid mesh and an inner component filled with collagen sponge promote and retain adipogenesis without the addition of exogenous cells or growth factors. In this study, we evaluated adipogenesis over time histologically and at the gene expression level
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Mass production of iPSC-derived platelets toward the clinical application Regen. Ther. (IF 4.3) Pub Date : 2024-01-04 Akihiro Kayama, Koji Eto
The production of platelets from induced pluripotent cells (iPSCs) may offer a safer and sustainable alternative for transfusions and drug delivery systems (DDS). However, the mass production of the clinically required number of iPSC-derived platelets (iPSC-PLTs) is challenging. Here, we introduce recent technologies for mass production and the first-in-human clinical trial using iPSC-PLTs. To this
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Long term improvement of knee osteoarthritis after injection of single high/very high volume of very pure PRP: A retrospective analysis of patients optimally managed in dedicated centers Regen. Ther. (IF 4.3) Pub Date : 2024-01-03 Didier Prost, Thomas Bardot, Alexandre Baud, Anthony Calvo, Stephane Aumont, Herve Collado, Julien Borne, Olivier Rajon, Antoine Ponsot, Alexandra Malaterre, Yannis Dahak, Guy Magalon, Florence Sabatier, Jeremy Magalon
PRP is gaining increasing interest for pain relief and improvement of joint function in patients with knee osteoarthritis (KOA) but practices and results remain heterogeneous limiting its adoption as standard of care. Current international recommendations are to collect real-life evidence of efficacy with a systematic monitoring of PRP quality and patients' outcomes. We aimed to analyze the response
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In vitro and functional investigation reveals the curative effect of thymoquinone from black cumin-loaded chitosan nanoparticles on streptozotocin induced paediatric diabetes Regen. Ther. (IF 4.3) Pub Date : 2023-12-31 Qiuyan Xue, Yingrong Lin
Diabetic ketoacidosis (DKA) is regarded to be a communal complication of both type 1 and type 2 diabetes mellitus in children and adolescents. Successful therapy of DKA in children requires prompt diagnosis, strict monitoring of medical indicators, and prompt action. Thymoquinone (Tq) from black cumin loaded chitosan nanoparticles (ChNPs) intend to assess an effective agent to overcome this problem
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The essential role of Mkx in periodontal ligament on the metabolism of alveolar bone and cementum Regen. Ther. (IF 4.3) Pub Date : 2023-12-29 Lisa Yagasaki, Tomoki Chiba, Ryota Kurimoto, Mitsuyo Nakajima, Takanori Iwata, Hiroshi Asahara
The periodontium is a connective tissue which consists of periodontal ligament, alveolar bone, cementum and gingiva. Periodontal ligament (PDL) is a specialized connective tissue that connects the cementum – coating the surface of the tooth – to the alveolar bone. Mohawk homeobox ) is a transcription factor that is expressed in PDL, that is known to play a vital role in the development and homeostasis
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Bi-functional KIT-PR1P peptides combine with VEGF to protect ischemic kidney in rats by targeting to Kim-1 Regen. Ther. (IF 4.3) Pub Date : 2023-12-28 Runxue Zhou, Hang Liu, Xianglin Hou, Qi Liu, Shuwei Sun, Xiaoge Li, Wenxuan Cao, Weihong Nie, Chunying Shi, Wei Chen
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Advancements in drug-loaded hydrogel systems for bone defect repair Regen. Ther. (IF 4.3) Pub Date : 2023-12-28 Weipeng Shi, Yaping Jiang, Tingyu Wu, Yingze Zhang, Tao Li
Bone defects are primarily the result of high-energy trauma, pathological fractures, bone tumor resection, or infection debridement. The treatment of bone defects remains a huge clinical challenge. The current treatment options for bone defects include bone traction, autologous/allogeneic bone transplantation, gene therapy, and bone tissue engineering amongst others. With recent developments in the
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Investigating the effect of mesenchymal stem cells on the rate of clinical and pathological improvement of asthmatic lung in mouse model Regen. Ther. (IF 4.3) Pub Date : 2023-12-27 Kambiz Moghaddasi, Saeed Hesaraki, Farnoosh Arfaee, Seyyed Shamsadin Athari
Asthma is a pulmonary disease and its pathophysiology includes inflammation, obstruction, edema of the airways, and mucus secretions in the airways. Mesenchymal stem cells (MSCs) are self-renewal that use the therapeutic potential of these cells can be applied as treatments of asthma. In this study, the effect of Mesenchyme stem cells on asthma was investigated.
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The construction of a microenvironment with the vascular network by co-culturing fibroblasts and endothelial cells Regen. Ther. (IF 4.3) Pub Date : 2023-12-26 Tatsuwo Fujita, Taigo Yuki, Michiyo Honda
Extracellular matrix (ECM) synthesis and deposition in fibroblasts, and vascularization via endothelial cells are essential for successful tissue regeneration. Fibroblasts can produce both ECM, physical support for maintaining homeostasis, and bioactive molecules, such as growth factors and cytokines. Endothelial cells can secrete growth factors and form vascular networks that enable the supply of
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Synergistic effect of metformin and vitamin D3 on osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells under high d-glucose conditions Regen. Ther. (IF 4.3) Pub Date : 2023-12-26 Nhi Nguyen-Yen Ha, Thi Kim Tan Huynh, Ngoc Uyen Phuong Phan, Thi-Hiep Nguyen, Long Binh Vong, Nhu-Thuy Trinh
Vitamin D plays a vital role in bone health, with low levels of vitamin D being related to skeletal fragility, fractures, and metabolic disorders such as diabetes. Metformin is known as an antihyperglycemic agent for regulating blood sugar. A correlation between diabetes mellitus and osteoporosis is attracting considerable interest, and research to find the prevention and treatment is gradually being
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Exosomal microRNA-Based therapies for skin diseases Regen. Ther. (IF 4.3) Pub Date : 2023-12-23 Chen Jibing, Liang Weiping, Yang Yuwei, Feng Bingzheng, Xu Zhiran
Based on engineered cell/exosome technology and various skin-related animal models, exosomal microRNA (miRNA)-based therapies derived from natural exosomes have shown good therapeutic effects on nine skin diseases, including full-thickness skin defects, diabetic ulcers, skin burns, hypertrophic scars, psoriasis, systemic sclerosis, atopic dermatitis, skin aging, and hair loss. Comparative experimental
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Liver organoids and their application in liver cancer research Regen. Ther. (IF 4.3) Pub Date : 2023-12-23 Xinyu Song, Kaifei Hou, Hongyan Zhou, Jingyi Yang, Ting Cao, Jiayu Zhang
Liver cancer, a common and intractable liver-related disease, is a malignant tumor with a high morbidity, which needs a high treatment cost but still lacks perfect clinical treatment methods. Looking for an effective platform for liver cancer study and drug screening is urgent and important. Traditional analytical methods for liver disease studies mainly rely on the 2D cell culture and animal experiments
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Simultaneous binding of bFGF to both FGFR and integrin maintains properties of primed human induced pluripotent stem cells Regen. Ther. (IF 4.3) Pub Date : 2023-12-23 Yu-Shen Cheng, Yukimasa Taniguchi, Yasuhiro Yunoki, Satomi Masai, Mizuho Nogi, Hatsuki Doi, Kiyotoshi Sekiguchi, Masato Nakagawa
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Mesenchymal cells support the early retention of primary alveolar type 2 cells on acellular mouse lung scaffolds Regen. Ther. (IF 4.3) Pub Date : 2023-12-20 Daisuke Taniguchi, Mohammadali Ahmadipour, Anthony L. Eiliazadeh, Pascal Duchesneau, Takeshi Nagayasu, Siba Haykal, Golnaz Karoubi, Thomas K. Waddell
Tissue engineering approaches via repopulation of acellular biological grafts provide an exciting opportunity to generate lung grafts for transplantation. Alveolar type 2 (AT2) cells are a promising cell source for re-epithelialization. There are however inherent limitations with respect to their survival and growth, thus impeding their usability for tissue engineering applications. This study investigates
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Remote audit practice for inspection of structural and equipment standards for cell processing facilities under the act on the safety of regenerative medicine in Japan Regen. Ther. (IF 4.3) Pub Date : 2023-12-16 Masao Sasai, Mina Okamoto, Shunsuke Tanigawa, Satoshi Okada, Daisuke Sugiyama, Akira Myoui
The Act on the Safety of Regenerative Medicine enforced in Japan in 2014, regulates the manufacture of cellular processed products. However, with regards to the manufacturing facilities at medical institutions, only the submission of necessary documents is required for a license, and the need for third-party inspection has been highlighted. Remote activities are becoming more prominent with the spread
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CD73-positive pediatric urethral mesenchymal stem-like cell-derived small extracellular vesicles stimulate angiogenesis Regen. Ther. (IF 4.3) Pub Date : 2023-12-14 Shilin Zhang, Jierong Li, Chunjing Li, Xumin Xie, Jun He, Fengsheng Ling, Bowei Li, Huayan Wu, Zhilin Li, Jianwei Zhen, Guoqing Liu
Angiogenesis plays an important role in the repair of urethral injury, and stem cells and their secretomes can promote angiogenesis. We obtained pediatric urethral mesenchymal stem-like cells (PU-MSLCs) in an earlier study. This project studied the pro-angiogenic effect of PU-MSLC-derived small extracellular vesicles (PUMSLC-sEVs) and the underlying mechanisms. PUMSLCs and PUMSLC-sEVs were cultivated
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Enhanced axon outgrowth of spinal motor neurons in co-culturing with dorsal root ganglions antagonizes the growth inhibitory environment Regen. Ther. (IF 4.3) Pub Date : 2023-12-10 Zi-Xing Xu, Dan Xu, Fang Fang, Ying-Juan Fan, Bing Wu, Yu-Fan Chen, Hao-En Huang, Xin-Hao Huang, Yue-Hong Zhuang, Wei-Hong Xu
Forming a bridge made of functional axons to span the lesion is essential to reconstruct the motor circuitry following spinal cord injury (SCI). Dorsal root ganglion (DRG) axons are robust in axon growth and have been proved to facilitate the growth of cortical neurons in a process of axon-facilitated axon regeneration. However, whether DRG transplantation affects the axon outgrowth of spinal motor
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Safety and feasibility of locoregional platelet-rich plasma injection for iliopsoas impingement after total hip arthroplasty: A phase 1 prospective observational study Regen. Ther. (IF 4.3) Pub Date : 2023-11-29 Yasuhiro Homma, Sayuri Uchino, Masashi Nagao, Takanori Wakayama, Shin Fukusato, Tomonori Baba, Taiji Watari, Koju Hayashi, Yoshitomo Saita, Muneaki Ishijima
Although numerous studies have reported outcomes with various conservative approaches for the iliopsoas impingement after total hip arthroplasty (THA), reports on the use of locoregional autologous platelet rich plasma (PRP) injections for the iliopsoas impingement after THA are lacking. This phase 1 study therefore aimed to investigate the safety and feasibility of locoregional PRP injection for iliopsoas
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Development of triple-functionalized calcium phosphate nanoparticles as an advanced drug delivery system for bone tissue repair Regen. Ther. (IF 4.3) Pub Date : 2023-11-28 Taichi Tenkumo, Benedikt Kruse, Kathrin Kostka, Viktoriya Sokolova, Toru Ogawa, Nobuhiro Yoda, Oleg Prymak, Osamu Suzuki, Keiichi Sasaki, Matthias Epple
During tissue repair or regeneration, several bioactive molecules are released and interact with each other and act as complex additives or inhibitors for tissue reconstruction. In this study, the bone-healing effects of the combination treatment with tumor necrosis factor-α (TNF-α) inhibition, vascular endothelial growth factor A (VEGF-A) and bone morphogenetic protein-7 (BMP-7) release by gene silencing
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Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury Regen. Ther. (IF 4.3) Pub Date : 2023-11-27 Jinghui Xu, Zhenxiao Ren, Tianzuo Niu, Siyuan Li
Mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) are promising therapies for the treatment of spinal cord injury (SCI). This study sought to explore the epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs in SCI. MSCs and MSCs-EVs were isolated and characterized. The SCI rat model was established, followed by Basso-Beattie-Bresnahan scoring and H&E staining. In vitro cell models were
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Injectable phase-separated tetra-armed poly(ethylene glycol) hydrogel scaffold allows sustained release of growth factors to enhance the repair of critical bone defects Regen. Ther. (IF 4.3) Pub Date : 2023-11-25 Shant Nepal, Jinyan Si, Shohei Ishikawa, Masaki Nishikawa, Yasuyuki Sakai, Aya M. Akimoto, Hiroyuki Okada, Shinsuke Ohba, Ung-il Chung, Takamasa Sakai, Hironori Hojo
With the rising prevalence of bone-related injuries, it is crucial to improve treatments for fractures and defects. Tissue engineering offers a promising solution in the form of injectable hydrogel scaffolds that can sustain the release of growth factors like bone morphogenetic protein-2 (BMP-2) for bone repair. Recently, we discovered that tetra-PEG hydrogels (Tetra gels) undergo gel-gel phase separation
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Application and challenges of stem cells in cardiovascular aging Regen. Ther. (IF 4.3) Pub Date : 2023-11-25 He-Ling Yuan, Le Chang, Wei-Wen Fan, Xin Liu, Qiang Li, Chuan Tian, Jing Zhao, Zi-An Li, Xing-Hua Pan, Xiang-Qing Zhu
With the rapid development of society and the economy, population aging has become a common challenge faced by many countries in the world today. Structural and functional changes in the cardiovascular system can occur with age, increasing the incidence and severity of cardiovascular diseases in older adults. Due to the limited regenerative capacity of myocardial cells, myocardial infarction and its
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Tissue engineering and stem cell-based therapeutic strategies for premature ovarian insufficiency Regen. Ther. (IF 4.3) Pub Date : 2023-11-25 Fatemeh Kuchakzadeh, Jafar Ai, Somayeh Ebrahimi-Barough
Premature ovarian insufficiency (POI), also known as premature ovarian failure (POF), is a complex endocrine disease that commonly affects women under the age of 40. It is characterized by the cessation of ovarian function before the age of 40, leading to infertility and hormonal imbalances. The currently available treatment options for POI are limited and often ineffective. Tissue engineering and
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Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability. Regen. Ther. (IF 4.3) Pub Date : 2023-11-21 Ryoko Inaki,Yoshihiko Sato,Daisuke Nakamura,Yoshiyuki Aikawa,Tsuyoshi Takato,Kazuto Hoshi,Atsuhiko Hikita
Background Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In
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Characterization of the cellular heterogeneity and bone regenerative potential of cultured human periosteal cells Regen. Ther. (IF 4.3) Pub Date : 2023-11-17 Takahiro Tsuzuno, Naoki Takahashi, Masaki Nagata, Yuta Ueda, Shunya Motosugi, Aoi Yamada, Mai Mizuguchi, Tran Thi Thuy Diep, Yukari Aoki-Nonaka, Koh Nakata, Koichi Tabeta
Introduction Cell-based bone regenerative therapy exhibits considerable potential in the treatment of bone defects caused by trauma, disease, and congenital anomalies. The periosteum, a fibrous membrane covering the outer surface of bone, plays a crucial role in bone formation and regeneration by sourcing osteoprogenitor cells. The remarkable osteogenic potential of periosteal cells (PCs) has led to
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Co-overexpression of OPN, IGF-1 and CNTF augment the therapeutic effect of DPSC on spinal cord injury Regen. Ther. (IF 4.3) Pub Date : 2023-11-17 Ning Tao, Xiwen Dong, Chao Liu, Lin Lv, Fanqi Hu, Huan Zhang, Xue Li, Panpan Geng, Han Duan, Chu-Tse Wu, Hua Wang
Background aims Spinal cord injury (SCI) is one of the most complex and destructive diseases of the nervous system, which can lead to permanent loss of tactile perception. But existing treatment methods have limited effects. To establish a novel method that may be therapeutic in repairing the injured spinal cord, gene-modified dental pulp stem cells (DPSCs) were injected in situ. Methods Adenovirus
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An overview to nanocellulose clinical application: Biocompatibility and opportunities in disease treatment Regen. Ther. (IF 4.3) Pub Date : 2023-11-16 Kosar Malekpour, Ali Hazrati, Arezou Khosrojerdi, Leila Roshangar, Majid Ahmadi
Recently, the demand for organ transplantation has promptly increased due to the enhanced incidence of body organ failure, the increasing efficiency of transplantation, and the improvement in post-transplant outcomes. However, due to a lack of suitable organs for transplantation to fulfill current demand, significant organ shortage problems have emerged. Developing efficient technologies in combination
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3D printed PLGA/MgO/PDA composite scaffold by low-temperature deposition manufacturing for bone tissue engineering applications Regen. Ther. (IF 4.3) Pub Date : 2023-11-11 Liang Tan, Zhuofeng Ye, Weida Zhuang, Beini Mao, Hetong Li, Xiuwang Li, Jiachang Wu, Hongxun Sang
Introduction Bones are easily damaged. Biomimetic scaffolds are involved in tissue engineering. This study explored polydopamine (PDA)-coated poly lactic-co-glycolic acid (PLGA)-magnesium oxide (MgO) scaffold properties and its effects on bone marrow mesenchymal stem cells (BMSCs) osteogenic differentiation. Methods PLGA/MgO scaffolds were prepared by low-temperature 3D printing technology and PDA
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Non-apoptotic regulated cell death in Fuchs endothelial corneal dystrophy Regen. Ther. (IF 4.3) Pub Date : 2023-11-10 Saki Sakakura, Emi Inagaki, Tomoko Sayano, Risa Yamazaki, Noemi Fusaki, Shin Hatou, Masatoshi Hirayama, Kazuo Tsubota, Kazuno Negishi, Hideyuki Okano, Shigeto Shimmura
Introduction Fuchs endothelial corneal dystrophy (FECD) is the leading cause of corneal blindness in developed countries. Corneal endothelial cells in FECD are susceptive to oxidative stress, leading to mitochondrial dysfunction and cell death. Oxidative stress causes many forms of cell death including parthanatos, which is characterized by translocation of apoptosis-inducing factor (AIF) to the nucleus
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Enhancing osteogenic differentiation in adipose-derived mesenchymal stem cells with Near Infra-Red and Green Photobiomodulation Regen. Ther. (IF 4.3) Pub Date : 2023-11-10 Daniella Da Silva, Anine Crous, Heidi Abrahamse
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Early revascularization activates quiescent dental pulp stem cells following tooth replantation in mice Regen. Ther. (IF 4.3) Pub Date : 2023-11-06 Hiroto Sano, Kuniko Nakakura-Ohshima, Angela Quispe-Salcedo, Yasuo Okada, Takuichi Sato, Hayato Ohshima
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Transplantation of autologous bone marrow–derived mononuclear cells into cerebrospinal fluid in a canine model of spinal cord injury Regen. Ther. (IF 4.3) Pub Date : 2023-11-04 Yuya Nakamoto, Tatsuo Nakamura, Ryusuke Nakai, Takashi Azuma, Koichi Omori
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Enhanced recellularization by using albumin coating with roller bottle cell culture Regen. Ther. (IF 4.3) Pub Date : 2023-10-31 Boyun Kim, Hyunwoo Jo, Bo Young Choi, Jina Ryu
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Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet Regen. Ther. (IF 4.3) Pub Date : 2023-10-13 Shutaro Yamamoto, Kenji Matsui, Yoshitaka Kinoshita, , Hidekazu Sekine, Yatsumu Saito, Yasuhide Nakayama, Haruki Kume, Takahiro Kimura, Takashi Yokoo, Eiji Kobayashi
Introduction Ureteral injuries require surgical intervention as they lead to loss of renal function. The current reconstructive techniques for long ureteral defects are problematic. Consequently, this study aimed to reconstruct the ureter in a rat model using subcutaneously prepared autologous collagen tubes (Biotubes). Methods The lower ureter of LEW/SsNSlc rats was ligated to dilate the ureter to
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Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives Regen. Ther. (IF 4.3) Pub Date : 2023-10-10 Yuki Ikeda, Shoichiro Tani, Takeshi Moriishi, Aiko Kuroda, Yuki Matsuo, Naoya Saeki, Chizuko Inui-Yamamoto, Makoto Abe, Takashi Maeda, David W. Rowe, Ung-il Chung, Hironori Hojo, Yuki Matsushita, Takashi Sawase, Shinsuke Ohba
Vertebrates form their skeletal tissues from three distinct origins (the neural crest, paraxial mesoderm, and lateral plate mesoderm) through two distinct modes of ossification (intramembranous and endochondral ossification). Since the paraxial mesoderm generates both intramembranous and endochondral bones, it is thought to give rise to both osteoprogenitors and osteo-chondroprogenitors. However, it