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Impact of Dissolved Oxygen Levels on N2O Emissions and Metabolically Active Bacterial Community in Biological Nitrogen Removal Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-14 Wanli Yan, Jianguo Li, Jieping Liang, Chengsong Ye, Xin Yu
Biological nutrient removal (BNR) is an effective process for removing nitrogen from wastewater using microorganisms under different environmental conditions. However, these processes can result in nitrous oxide (NO) emission. Dissolved oxygen (DO) levels shape bacterial communities and affect NO emissions in BNR. Ammonia-oxidizing bacteria (AOB), the main NO producers in the BNR process, are significantly
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Zinc oxide-gold nanocomposite modification on dielectrode for diagnosing osteosarcoma by dual probes Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-13 Tian Zhoubin, Wang Yanyan, Wu Wenqiang, Hou Chengtai, Wu Shuai
Osteosarcoma is a malignant bone tumor, originating and developing from mesenchymal tissues. Diagnosis of osteosarcoma is generally carried out by tissue biopsy and radiological investigations, which are painful and expensive. Therefore, researchers are focusing on identifying the biomarkers for osteosarcoma diagnosis. Matrix metalloproteinases (MMP) have been proven to be involved in the regulation
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Dual cytoplasmic-mitochondrial engineering for improved patchoulol production in Komagataella phaffii Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-13 Guangjuan Luo, Ying Lin, Shupeng Ruan, Ruiming Xiao, Xinying Zhang, Shuli Liang
Mitochondria are a potential sub-organelle for the synthesis of terpenoids due to its abundant acetyl-CoA, cofactors, and compact compartment for targeting metabolic pathways and enzymes. Hence, conducting systematic investigations and harnessing the subcellular compartment of mitochondria may offer a viable avenue for enhancing terpenoids biosynthesis in yeast and other eukaryotic cells. Previous
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Biobased propylene glycol production in a sugarcane biorefinery through lactic acid, glycerol, or sorbitol: A techno-economic and environmental evaluation of intermediates and downstream processing methods Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-11 Mensah S. Brobbey, Jeanne Louw, Johann F. Görgens
Propylene glycol (PG) is a versatile chemical which can be produced by direct fermentation of sugars or through intermediates like lactic acid (LA), glycerol and sorbitol. Few studies have examined producing PG from different intermediates in terms of economic feasibility. In this study, seven process scenarios were assessed in a biorefinery annexed to an existing sugar mill. Aspen simulations were
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The impact of reduced sulfur compounds on aerobic granular sludge formation and biological phosphorus removal Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-09 Karina Seguel Suazo, Thomas Dobbeleers, Jan Dries
Biological sulfur conversion combined with phosphorus removal represents an innovative biotechnology for activated sludge (AS). However, its effect on the formation of aerobic granular sludge (AGS) remains unclear. This study aimed at determining the impact of sulfide and thiosulfate on the performance of enhanced biological phosphorus removal (EBPR) and on AGS formation. Using two lab-scale sequencing
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Microwave-assisted synthesis of porous graphitized biochar as a super sorbent for tetracycline separation Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-07 Huihao Wang, C. Srinivasakannan, Meijing Liu, Gang Xue, Li Wang, Yaping Wang, Xin Wang, Xinhui Duan
Porous graphitized biochar was synthesized from waste coconut shell utilizing microwave-assisted pyrolysis, with combination of KOH and KCl as activators. The activators s not only facilitated creation of porous and graphitized structure with high surface area, but also reduced the KOH corrosion as an impeding factor against KOH activation. The process further facilitates easy recovery and reuse of
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Enhanced biomass and lipid production from olive processing wastewater using Scenedesmus obliquus in a two-stage cultivation strategy under salt stress Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-07 Andonia Nicodemou, Despoina Konstantinou, Michalis Koutinas
The study enhanced cultivation via optimization of C/N and C/P ratios in synthetic media and evaluation of a two-stage cultivation strategy through application of salinity stress in olive processing wastewater-based feedstocks. under 40/1 C/N ratio performed biomass and lipid productivity that reached 0.15 and 34.1 mg L d respectively, demonstrating high glucose removal efficiency. Application of a
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Metabolic Engineering of Bcat1, Adh5 and Hahdb Towards Controlling Metabolic Inhibitors and Improving Performance in CHO Cell-Cultures Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-05 Bingyu Kuang, Duc Hoang, Qiang Fu, SoYoung Park, George Liang, Seongkyu Yoon
CHO cells are known to secrete growth inhibitory metabolites during growth and production phases, which hampers cellular performance and negatively impacts final productivity and product quality attributes. Previous studies have identified different metabolic by-products derived from CHO metabolism and demonstrated their negative impacts on growth and titer productivity. This work presents a control
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Odorant-binding proteins as recognition elements for smell exploration Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-03 Yi Wang, Chuanting Qin, Xiaoxue Wang, Ting Wang, Jian Dong, Yuan Lu
The sense of smell in animals enables them to detect and discriminate among many volatile odor molecules in complex environments. This task is accomplished by the family of G-proteins (olfactory receptor proteins, odorant-binding proteins, and chemosensory proteins). Here, we mechanistically elucidate how odors bind to odorant-binding proteins (OBPs) by studying the binding properties of 50 odorant
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Nano-Differential Scanning Fluorimetry as a tool for assessment of refolded antibody fragments: a case study for anti-Pfs25 single-chain antibodies. Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-02 Harvinder Khera, Ashwathi Valiyaparambil, Deepak K Jagannath, Vysakh K Viswanath, Naveen Kumar, Jay Prakash Shukla, Sabyasachi Pradhan, Anirudha Lakshminarasimhan
Differential Scanning Fluorimetry (DSF) is a valuable and versatile tool in the field of protein and antibody stability studies, providing valuable insights into their thermal stability and aids in the optimization of experimental conditions for varied applications. Antibody fragments provide armamentarium for researchers and practitioners across various fields, enabling advancements in medicine, diagnostics
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Functional enzyme analysis and metabolic regulation mechanism of the combined microflora LXB in the degradation of lignocellulose Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-02 Yuhao Hao, Mengling Lu, Yu Zhang, Binfeng Lin, Liang Chen, Zhi Huang, Kai Li, Jianbin Li
Biodegradation of lignocellulosic biomass is a practical and effective method. A random combination of six isolates and two purchased strains was constructed to obtain LXB that exhibited a lignocellulose degradation rate of 36.4% after 168hours. The Carbohydrate-Active enZYmes Database identified 1,872 genes that were differentially expressed and involved in the degradation of lignocellulose in LXB
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A Model-Based Performance Analysis of Integrated Chromatography-Ultrafilter Separation Systems for Monoclonal Antibody (mAb) Manufacturing Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-02 Wil Jones, Dimitrios I. Gerogiorgis
Robust and accurate modelling of bioprocess systems offers the opportunity to visualise strategies for rapid-scale up of production platforms from benchtop to industrial scale, but few studies investigate how mechanistic and hybrid models can be used to design integrated bio-manufacturing. This is especially true for downstream purification technologies used to separate mAbs from culture by-products
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Separation and enrichment of multiple bile acid micelle-disrupting peptides by adsorption/desorption process with heat-treated porous silica gels Biochem. Eng. J. (IF 3.9) Pub Date : 2024-03-02 Momoha Iriyama, Hitomi Hagawa, Shota Shimizu, Hirokazu Akiyama, Kazunori Shimizu, Hiroyuki Honda
Bile acid (BA) micelle-disrupting peptides are bioactive peptides (BPs) that suppress intestinal cholesterol adsorption and ameliorate blood cholesterol levels. To facilitate their application in the food industry, an effective and straightforward technology for BP separation and enrichment of BPs is required. For this purpose, we assessed the potential of heat-treated (HT) porous silica gels with
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Evolutionary adaptation in the laboratory and the process of retrograde engineering augment autotrophic proliferation in Saccharomyces cerevisiae Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-28 Seyed Mehrdad Mirsalami, Mahsa Mirsalami
Genetic engineering provides various avenues for CO transformation into biological matter or bio-substances, aiding in mitigating the unsustainable utilization of organic raw materials that contribute detrimentally to global warming. The employment of widely recognized industrial microorganisms, like , for the creation of innovative carbon (C) biomanufacturing frameworks may detach biological technology
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Biomethane recovery performance and microbial community dynamics of a high-biomass submerged AnMBR (HBSAnMBR) treating abattoir wastewater Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-28 Rajneesh Kumar Gautam, Nuwan Vithanage, Nandkishor More, Shobha Muthukumaran, Dimuth Navaratna
This study investigated the treatment and biomethane recovery performance of a high-biomass submerged anaerobic membrane bioreactor (HBSAnMBR) treating abattoir wastewater in six operational phases (Phases 1 – 6) at an organic loading rate (OLR) range of 1.05 – 7 kg-COD/m/d. The HBSAnMBR system demonstrated a biomethane recovery of 75.5 ± 2.0% and COD removal efficiency of 98.8 ± 0.71% during the most
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Effect of zero-valent iron addition on ammonia inhibition alleviation and fecal indicators reduction in anaerobic digestion of pig manure Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-28 Hongyuan Sun, Rui Tang, Kuizu Su, Shoujun Yuan, Jingwei Feng, Wei Wang, Zhen-Hu Hu
Anaerobic digestion (AD) is widely used for the treatment of pig manure, but is easily suffered from ammonia inhibition. The residual pathogenic bacteria and antibiotic resistance genes in the digestate pose potential risks to human health and the environment. Zero-valent iron (ZVI) addition in AD has been extensively investigated to enhance AD performance, but little is known about the impacts of
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Current strategies for the development of high-yield HEK293 cell lines Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-24 Liao Zhang, Jianhui Gao, Xi Zhang, Xiaoyin Wang, Tianyun Wang, Junhe Zhang
At present, approximately 80% of the currently approved recombinant therapeutic proteins are produced by Chinese hamster ovary cells. However, considering that the human embryonic kidney (HEK) 293 cell line comes from human sources and has the advantages of high transfection rate, fast growth rate, good post-translational modifications, and growth rate in serum-free suspension culture, the HEK293 cell
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Electric-field-assisted in situ separation of mandelic acid in an enzymatic milli-reactor improves the control of reaction conversion and enhances the enantiomeric ratio Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-24 Lukáš Sauer, Dominik Kralik, Zdeněk Slouka, Michal Přibyl
Enantiopure or enantiomerically enriched chemicals are often used as active pharmaceutical ingredients (APIs). However, API synthesis and purification on a preparative scale is usually costly, time-consuming, and difficult to scale up in conventional batch reactors. To develop an alternative solution, we applied kinetic enantioselective synthesis with simultaneous product separation. More specifically
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Increasing biosensor-based cell selection pressure improves microbial biosynthesis of 4-hydroxybenzoate Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-23 Yuxin Liu, Xiaonan Wang, Lei Zhuang, Mia Stapleton, Haoran Zhang
Biosensors are powerful tools for improving microbial biosynthesis. For example, biosensors can be used to establish feedback control mechanisms to inhibit the growth of low performing cells in a microbial population. In this study, a previously developed biosensor-assisted cell selection system was further engineered to increase the cell selection pressure for improving the bioproduction of 4-hydroxybenzoate
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Unravelling the role of coumarin on anaerobic digestion of waste activated sludge: Determination of operational dynamics and microbial community structure Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-23 Hongmei Ye, Shuai Tian, Zuwen Liu, Yichun Zhu, Jincai Luo, Qing Mao
Anaerobic digestion, as an effective method for sludge treatment, still has excellent potential for development in energy recovery. Therefore, this study proposed a method to increase methane production by adding coumarin (a green antibacterial agent). The results indicated that coumarin can significantly improve the methane production performance of sludge, but high coumarin concentrations delay methane
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Evaluation of antimicrobial effect of bacteriocin produced by Lactobacillus acidophilus utilizing molasses and corn steep liquor Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-17 Sayyid Zulhelmie Shahrir, Phei Er Kee, Hui Suan Ng, Joo Shun Tan, John Chi-Wei Lan
Bacteriocins are antimicrobial peptides produced by lactic acid bacteria (LAB) which have emerged as safe bio-preservatives in the food industry. However, the conventional commercial medium used to promote bacteriocin production is often costly and impractical for industrial-scale production. This study aimed to explore the utilization of industrial by-products, specifically molasses and corn steep
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Cordyceps fungus-mediated enantioselective synthesis of chiral alcohol and enhanced biocatalytic efficiency in cyclodextrin/surfactant-buffer system Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-17 Qian Gui, Hai-Min Zhang, Feng Qian, Han-Yu Liu, Xiang-Xin Kong, Guo-Ying Lv, Pu Wang
Asymmetric synthesis of chiral alcohols by biocatalysts holds promising prospect. In this study, a medicinal fungus, namely , was adopted as a novel biocatalyst for the synthesis of chiral alcohols. Among the assessed prochiral ketone substrates, exhibited the best catalytic activity towards 1-(4-bromophenyl)-2,2,2-trifluoroethanone (BPFO), yielding a valuable pharmaceutical intermediate ()-1-(4-bromophenyl)-2
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Characterization, production optimization and ecotoxicity of a lipopeptide biosurfactant by Pseudomonas citronellolis using oily wastewater Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-15 Constantina K. Varnava, Paola Grenni, Livia Mariani, Anna Barra Caracciolo, Haria Hadjipakkou, George Lefkaritis, Eftychia Pinakoulaki, Nikos Chronakis, Nikolaos Kalogerakis, Argyro Tsipa
Considering both fundamental principles and application perspectives, it is imperative to characterize biosurfactants (BSFs) for replacing their synthetic counterparts. In this study, a BSF produced by the 620 C bacterium, using oily wastewater, was identified as a lipopeptide based on ultraviolet spectroscopy, thin layer chromatography, high-pressure liquid chromatography and nuclear magnetic resonance
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Dual coiled-coil protein domain mimic and drug delivery vehicle for SARS-CoV-2 Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-15 Dustin Britton, Chengliang Liu, Sihan Jia, Deven Paul, Jakub Legocki, Yingxin Xiao, Xunqing Jiang, Xiang-Peng Kong, Jin Kim Montclare
SARS-CoV-2 has emerged as a strong target for the development of protein domain mimics (PDMs) as it relies on the helical protein-protein interaction (PPI) between the N-terminal α-helix of ACE2, ACE, with the SARS-CoV-2 receptor binding domain (RBD). We have recently developed an ACE-based multivalent assembled protein (ACE-MAP) that relies on fusion of a binding domain to the cartilage oligomeric
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Synergistic fermenter-electrolytic cell integration and phenomena-based process intensification for competitive bio-succinic acid production Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-15 Alina Anamaria Malanca, Ioannis V. Skiadas, Hariklia N. Gavala, Manuel Pinelo, Seyed Soheil Mansouri
The production of bio-succinic acid through fermentation presents a promising strategy for obtaining building-block chemicals from renewable sources. However, the limited yield of bio-succinic acid from fermentation and the complex purification procedures have hindered its competitiveness against petroleum-based succinic acid. One way to tackle this challenge is to develop a more efficient and sustainable
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Different inhibition patterns of ammonia and salt revealed by kinetic analysis of their combined inhibitory effect on methanogenesis of hydrogen: A preliminary study Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-15 Zi-Yan Li, Shintaro Nagao, Daisuke Inoue, Michihiko Ike
Kinetic parameters of the inhibitory effect of ammonia and salt on anaerobic digestion remain unclear. We investigated the combined inhibition effect of ammonia and salt on anaerobic digestion through hydrogen-fed specific methanogenetic activity (SMA) tests. The kinetic parameters estimated using mathematical models indicated a concentration-dependent monotonical SMA decrease caused by both ammonia
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A paradigm shift for biocatalytic microreactors: Decoupling application from reactor design Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-14 Marijan Bajić, Sansanee Khiawjan, Stephen T. Hilton, Gary J. Lye, Marco P.C. Marques, Nicolas Szita
Microreactors have been successfully applied to execute a broad range of biotransformations in flow. However, microreactors have typically been designed with a specific biotransformation or a specific biocatalyst immobilization method in mind, constraining their wider applicability. Furthermore, their design is typically either applicable for whole-cell or for enzyme biocatalysis, but not for both
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A precision miRNA-21 detection method based on self-powered biosensors Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-14 Hui Li, Jing Xu, Guanghui Chen, Huiling Wang, Zongshan Zhang
Early detection and treatment are effective strategies to reduce cancer mortality. Self-powered biosensors based on enzyme biofuel cells are rapid, high-sensitivity, and low-cost analysis methods with various detection modes, demonstrating great advantages as detection platforms for liquid biopsy. Aiming to further improve the detection sensitivity of self-powered biosensor platforms, this study adopted
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Influence of initial glucose and lactic acid concentration on hyaluronic acid production by Streptococcus zooepidemicus: Kinetic and modeling Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-13 David Antonio Flores-Méndez, Miriam Granados-Vallejo, Guadalupe María Guatemala-Morales, Rosa Isela Corona-González, Carlos Pelayo-Ortiz, Enrique Arriola-Guevara
Hyaluronic acid (HA) is a molecule of significant commercial value with applications in the pharmaceutical, cosmetic, and medical industries. It is typically produced through microbial fermentation. However, the efficiency of this type of process can be affected by inhibition phenomena due to the substrate or the product. In this sense, the effect of different initial concentrations of glucose (9.3
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Renewable gases production coupled to synthetic wastewater treatment through a microbial electrolysis cell Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-12 Lorenzo Cristiani, Marco Zeppilli, Giuliano Fazi, Clara Marandola, Marianna Villano
This study describes the use of a microbial electrolysis cells for the production of gaseous biofuels sustained by the oxidation of a synthetic wastewater. During the overall experimental investigation, the MEC’s bioanode removed on average 855 ± 57 mgCOD/d producing an average electric current of 66 ± 7mA which was diverted into gaseous biofuels like biomethane, biohydrogen and biohythane. Three different
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Immobilization of poly(hexamethylene biguanide) to cellulose acetate- and cellulose-based nanofiber membranes for antibacterial and cytotoxic studies Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-12 Dinh Thi My Huong, Chi-Yun Wang, Pin-Yi Chen, Chien Wei Ooi, Xue Er Crystal Thew, Bing-Lan Liu, Chen-Yaw Chiu, Shen-Long Tsai, Kuei-Hsiang Chen, Yu-Kaung Chang
To develop the environmental antibacterial membrane, the physical attachments of poly (hexamethylene biguanide) hydrochloride (PHMB) on the cellulose acetate (CA) and regenerated cellulose (RC) electrospun nanofiber membranes were employed. The immobilization of PHMB increased the antibacterial efficacy (, %) of nanofiber membranes from 65.67% to approximately 86.13% for CA-PHMB and from 35.09% to
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Characterization, degradation pathway and microbial community of aerobic granular sludge with ciprofloxacin at environmentally relevant concentrations Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-10 Xia Zhao, Xiran Li, Weina Pei, Xinyi Li, Qian Wei, Weijing Liu
The removal effect of domesticated aerobic granular sludge on ciprofloxacin (CIP) was studied in SBR system. The morphology and microstructure of cultivated and mature granular sludge were detected by SEM and XRD, and the "crystal nucleus hypothesis" was confirmed as the main reason for the formation of granular sludge. The efficiency of granular sludge to treat conventional pollutant’s COD, NH- N
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Methanogenesis performance and microbial characteristics in An-IFFAS process filled with conductive suspended carriers at ambient temperature Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-10 Xiangqian Guan, Tao Liu, Nan Wu, Xie Quan
Direct interspecies electron transfer (DIET) has been proven to be established efficiently on conductive suspended carriers, and thus enhances methane production at thermophilic condition. However, the methanogenesis performance and relevant microbial characteristics in the anaerobic integrated floating-film activated sludge (An-IFFAS) reactor filled with conductive suspended carriers when feeding
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Two-step cultivation of Neochloris oleoabundans in a novel horizontal thin-layer algal reactor: Interplay of pH and dissolved inorganic carbon Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-09 Hongying Zhou, Ju Wang, Zitong Xu, Xinyue Wang, Zisheng Zhang, Christopher Q. Lan
This study explored the impacts of culture pH and addition of NaHCO on the growth of an oil-rich green alga It was observed that in the absence of aeration, the culture pH fluctuated over wide ranges during the day/night cycles without additional DIC, which was flatted significantly by adding DIC in culture, demonstrating the effectiveness of DIC as a pH buffer. Addition of DIC in the range of 80-320mM
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Engineering artificial fusion naringinase for enhancing naringenin biosynthesis Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-09 Chen-Mu Luo, Wen-Ting Zhang, Song-Yi Xie, Xiao-Yan Zhuang, Ze-Wang Guo, Qiong Xiao, Jun Chen, Fu-Quan Chen, Qiu-Ming Yang, Yi Ru, Hui-Fen Weng, An-Feng Xiao, Yong-Hui Zhang
Naringinase (NGase) is a multi-compound enzyme with the subunits of α-L-rhamnosidase (Rha) and β-D-glucosidase (BGL). The biotransformation of naringin into the more valuable naringenin was impeded by the uncoordinated activity and instability of natural naringinases. In this study, the efficient artificial fusion naringinases of Rha from (StRha) and BGL from (PfBGL) were constructed and utilized in
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Application of ensemble learning to augment fluorescence-based PAT and enable real-time monitoring of protein refolding Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-08 Rashmi Sharma, Naveen G Jesubalan, Anurag S Rathore
Recently, there has been a noticeable increase in the attention given to Process Analytical Technology (PAT) within biopharmaceutical manufacturing. Specifically, there has been a focus on using spectroscopic tools in conjunction with chemometrics analysis. The emergence of computational advancements, such as artificial intelligence and machine learning, has resulted in a shift away from traditional
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Numerical simulation on vortex flow and CO2 bubble aggregation behavior in a raceway pond with diaphragm aerators Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-07 Xiangbo Zou, Mumin Rao, Ji Ye, Cao Kuang, Cong Huang, Gongda Chen, Shiwei Qin, Yanmei Song, Dongwei Jia, Jun Cheng
To optimize flow field in a raceway pond with diaphragm aerators to promote microalgal CO fixation, numerical simulation on three-phases (CO bubbles-culture medium-microalgae cells) with CFD (Computational Fluid Dynamics) - PBM (population balance model) was conducted to investigate effects of dynamic bubble aggregation/fragmentation on flow field distribution and mixing performance. The inhomogeneity
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Production of poly-γ-glutamic acid from lignocellulosic biomass: Exploring the state of the art Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-07 Somiame Itseme Okuofu, Vincent O’Flaherty, Olivia McAuliffe
Poly-γ-glutamic acid is an important biopolymer with attractive properties of commercial value across several industries. However, its high market price prohibits its widespread application. The utilisation of lignocellulosic biomass as an alternative carbon source for poly-γ-glutamic acid production could be beneficial from a cost and sustainability perspective. This review examines the state of the
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Mycelium-bound lipases of fungi as biocatalysts for biodiesel synthesis – A proposal to use FTIR as a rapid screening method Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-07 Mateusz Kutyła, Natalia Jaszek, Wiktoria Jędrys, Sandra Graba, Ewelina Pluta, Katarzyna Gdula, Aleksandra Batyra, Amelia Szczepańska, Alicja Śliwa, Laura Cieślak, Anna Marzec-Grządziel, Mariusz Trytek
In this study, biocatalysts in the form of mycelium-bound lipases of psychrotrophic and mesophilic fungi were screened for their ability to synthesize fatty acid methyl (FAME) and ethyl esters (FAEE). It has been shown that the hydrolytic activity of biocatalysts is not accompanied by their biodiesel synthetic activity. Preliminary studies showed for the first time, that the FTIR technique can be used
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Statistical optimization of cassava starch medium for D-lactic acid production using Leuconostoc mesenteroides ZL01 Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-06 Zixing Wang, Huixiong Zhong, Jianing Li, Mengyuan Jiang, Anqi Li, Keke Cheng
The purpose of this study was to investigate a D-lactic acid production process from cassava with high optical purity, high yield, and without by-products. In order to improve the lactic acid production capability of ZL01, the Plackett-Burman and Box-Behnken Design with Response Surface Methodology approach were employed to optimize the media composition. The lactic acid concentration in shake flask
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Transcription factor based whole-cell biosensor for inosinic acid in Corynebacterium stationis Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-06 Shibo Jiang, Zhilin Ouyang, Youhua Cai, Ying Lin, Suiping Zheng
IMP(Inosinic acid) is a raw material with extremely important uses in food manufacturing and agriculture. In the selection process of IMP producing strains, there has been no tool available to assist in high-throughput screening, which greatly affects the speed of strain development. To address this issue, we have developed a whole cell IMP biosensor based on the characteristics of , a member of the
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Removing bacteria contaminants from raw surface water using multiple barriers pilot-scale reactor: sand filtration, activated carbon, and advanced oxidation processes Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-05 Kassim Chabi, Chengsong Ye, Wanli Yan, Jianguo Li, Claude Kiki, Li Xianzhong, Zheng Shikan, Mahmoud Gad, Xin Yu
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Generation of a hetero-dye-labeled Quenchbody against programmed cell death-ligand 1 Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-03 Sun-Hee Kim, Hae-Min Park, Hee-Jin Jeong
The development of a simple and rapid technique for detecting programmed cell death-ligand 1 (PDL1) is crucial for cancer diagnosis and therapeutic monitoring. In this study, a novel Quenchbody (Q-body) was developed for rapid and high-throughput one-pot detection of PDL1. Specifically, a new labeling method using both Cystagging and Sortagging was employed to conjugate two dyes of different colors
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Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-02 Sebahat Ascioglu, Elif Ozyilmaz, Ayse Yildirim, Serkan Sayin, Mustafa Yilmaz
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Enzymatic synthesis of TMP esters based on pelargonic acid from the cleavage of oleic acid: Evaluation of synthetic process, physicochemical properties and lubrication performance Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-02 Qian Mao, Changliu He, Biqiang Chen, Xu Zhang
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Hydrothermal Liquefaction of Chlamydomonas nivalis and Nannochloropsis gaditana Microalgae Under Different Operating Conditions Over Copper-Exchanged Zeolites Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-02 H. Yousefzadeh, E. Borhan, B.Z. Haznedaroglu, A. Uzun, C. Erkey
In this study, two different green microalgae, Chlamydomonas nivalis (C. nivalis) and Nannochloropsis gaditana (N. gaditana), were cultivated in open ponds and the harvested wet biomass was converted to bio-crude by hydrothermal liquefaction (HTL) with/without catalyst. Catalytic HTL experiments were performed by using copper-exchanged zeolites including Cu-MOR, Cu-ZSM-5, and Cu-SSZ13, synthesized
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An innovative strategy for regenerating anammox granule sludge: Nitrogen removal performance, sludge characteristics, and microbial community Biochem. Eng. J. (IF 3.9) Pub Date : 2024-02-02 Jianbo Wu, Yafeng Li, Chi Zhang, Zhibo Wang, Siding Xu
To prevent sludge floating, a new strategy known as "breaking-collecting-recovering" has been proposed, which involves adding filler to the reactor, breaking the floating granules, and recycling them into the reactor. This allows the broken granules to be recovered and settle on the surface of the filler. In this study, granules were divided into three categories according to location: granules that
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Comparative study of chemical and enzymatic pre-treatments of Kitchen waste (KW) to generate fermentable sugars for the production of polyhydroxyalkanoates (PHA) Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-29 Shraddha Chavan, Bhoomika Yadav, R.D. Tyagi, Jonathan W.C. Wong, Patrick Drogui
Kitchen waste (KW) production is anticipated to rise because of accelerated urban and economic growth, particularly in Asian nations. Therefore, applications/recycling of KW should be explored for its effective utilization to generate a sustainable source of energy that will ultimately avoid massive economic and energy losses. The main goal of this study was to investigate the combinational effect
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Fermentation Strategies for PHB Production in a Novel Membrane Bioreactor: Investigating Batch and Fed-Batch Operations Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-29 Burcu Akkoyunlu, Sorcha Daly, Eoin Syron, Eoin Casey
Gas-transfer membranes have been successfully deployed as efficient aeration devices in wastewater treatment. There is an increasing interest in using such membrane technology in industrial biotechnology. This study proposes membrane bioreactors as a novel bioreactor setup for polyhydroxybutyrate (PHB) production using Cupriavidus necator, whereby gas-transfer membranes are used for aeration. A proof-of-concept
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Preparation of nitrogen-doped biocarbon derived from shrimp shell and its utilization in glucose biofuel cell Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-28 Yanhong Nie, Tianwen Huan, Jianrong Jiang, Yuhan Wang, Xiaoyan Huang, Decheng Bi, Xu Xu, Chaogang Wang, Zhangli Hu, Hong Xu
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A comparison of dual-enzyme immobilization by magnetic nanoparticles and magnetic enzyme aggregates for cascade enzyme reactions Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-22 Guanya He, Hairong Liu, Chengli Yang, Kaishun Hu, Xuchun Zhai, Beibei Fang, Kaixuan Liu, Dali Li Zulekha
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Rapid degradation of poly(butylene succinate-co-butylene terephthalate)s by microbial communities at high-temperature Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-24 Hongyan Shen, Ye Yuan, Chuang Liu, Maohua Yang, Jianmin Xing
Poly(butylene succinate-co-butylene terephthalate)s (PBST) is a partially biobased biodegradable plastic, prepared by polymerizing polybutylene succinate (PBS) and polybutylene terephthalate (PBT). Though confirmed to be biodegradable, research on its degradation, particularly at high temperatures, is limited. In this study, microbial communities obtained from diverse environmental samples were used
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Crucial roles of microbial community and in-situ synthetic Bio-Ru0 on diclofenac degradation and bioelectricity generation in a microbial fuel cell integrated electrocatalytic process Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-24 Chaoyang Tang, Bing Qiu, Yongyou Hu, Donghui Liang, Yuancai Chen, Jianhua Cheng
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Microbial community composition from full-scale reactors treating mature landfill leachate through innovative biological processes and its importance in mathematical modeling Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-19 Michela Langone, Roberta Ferrentino, Willy Mertz, Davide Mattioli, Luigi Petta, Gianni Andreottola
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Performance and biofilm characteristics of an anammox biofilter for treating low nitrogen wastewater at room temperature Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-15 Huilong OuYang, Yi Wang, Shirong Dong, Yuening Wang, Ting Yang, Lijuan Gao, Baohua Chai, Pengyu Zhuang, Xiaomei Kou, Wentao Li
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A synergistic association between iron reduction and enhanced hydrogen production in Clostridium pasteurianum Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-13 Parinita Mishra, Nilotpala Pradhan
Clostridium pasteurianum is one of the most promising naturally occurring microbes, which produces bio-hydrogen. The iron-reducing property of the bacteria is an advantageous process that enhances its hydrogen production efficiency. This review highlights the metabolic pathways encompassing the iron-reducing, carbon-oxidizing and hydrogen producing features of the bacteria. The structural and functional
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Heterologous expression and activity of α-helical antimicrobial peptide SW in Bacillus subtilis Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-14 Lu Zhao, Ling Li, Yinghan Xu, Mingyang Hu, Yuxin Fang, Na Dong
The antimicrobial peptide Cecropin P1 (CP1) was isolated from the pig-intestinal parasitic nematode Ascaris lumbricoides and had potent antimicrobial activity. However, its cytotoxicity is high. Herein, Using CP1 as the parent peptide, we designed a derivative peptide SW by replacing amino acids based on the structure and function relationship of antimicrobial peptides (AMPs) to reduce the toxicity
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Biorefinery-based sustainability assessment of macroalgae waste valorization to polylactic acid: Exergy and exergoeconomic perspectives Biochem. Eng. J. (IF 3.9) Pub Date : 2024-01-15 Yew Lun Tan, Inn Shi Tan, Henry Chee Yew Foo, Jibrail Kansedo, Man Kee Lam, Steven Lim, Pau Loke Show, Mee Kee Wong