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A long-term storable gel-laden chip composite built in a multi-well plate enabling in situ cell encapsulation for high-throughput liver model Biofabrication (IF 9.0) Pub Date : 2024-02-23 Min Kyeong Kim, Jubin Park, Sungho Tak, Kyurim Paek, Geul Bang, Sang-Mi Woo, Naresh Kumar Ravichandran, Won Gi Hong, Hyun-Wook Kang, Hyang Kim, Ji Yong Bae, Jeong Ah Kim
Hydrogels are widely used as scaffold materials for constructing in vitro three-dimensional microphysiological systems. However, their high sensitivity to various external cues hinders the development of hydrogel-laden, microscale, and high-throughput chips. Here, we have developed a long-term storable gel-laden chip composite built in a multi-well plate, which enables in situ cell encapsulation and
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Biofabrication of biomimetic undulating microtopography at the dermal-epidermal junction and its effects on the growth and differentiation of epidermal cells Biofabrication (IF 9.0) Pub Date : 2024-02-22 Chuang Gao, Chunxiang Lu, Huazhen Liu, Yi Zhang, Hao Qiao, Aoxiang Jin, Qiqi Dai, Yuanyuan Liu
The undulating microtopography located at the junction of the dermis and epidermis of the native skin is called rete ridges (RRs), which plays an important role in enhancing keratinocyte function, improving skin structure and stability, and providing three-dimensional (3D) microenvironment for skin cells. Despite some progress in recent years, most currently designed and manufactured tissue-engineered
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Analysis of bioprinting strategies for skin diseases and injuries through structural and temporal dynamics: historical perspectives, research hotspots, and emerging trends Biofabrication (IF 9.0) Pub Date : 2024-02-22 Fei Teng, Wei Wang, Zhi-Qiang Wang, Gui-Xue Wang
This study endeavors to investigate the progression, research focal points, and budding trends in the realm of skin bioprinting over the past decade from a structural and temporal dynamics standpoint. Scholarly articles on skin bioprinting were obtained from WoSCC. A series of bibliometric tools comprising R software, CiteSpace, HistCite, and an alluvial generator were employed to discern historical
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Co-axial hydrogel spinning for facile biofabrication of prostate cancer-like 3D models Biofabrication (IF 9.0) Pub Date : 2024-02-20 Carlos F Guimarães, Shiqin Liu, Jie Wang, Emma Purcell, Tugba Ozedirne, Tanchen Ren, Merve Aslan, Qingqing Yin, Rui L Reis, Tanya Stoyanova, Utkan Demirci
Glandular cancers are amongst the most prevalent types of cancer, which can develop in many different organs, presenting challenges in their detection as well as high treatment variability and failure rates. For that purpose, anticancer drugs are commonly tested in cancer cell lines grown in 2D tissue culture on plastic dishes in vitro, or in animal models in vivo. However, 2D culture models diverge
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Cell viability prediction and optimization in extrusion-based bioprinting via neural network-based Bayesian optimization models Biofabrication (IF 9.0) Pub Date : 2024-02-15 Dorsa Mohammadrezaei, Lena Podina, Johanna De Silva, Mohammad Kohandel
The fields of regenerative medicine and cancer modeling have witnessed tremendous growth in the application of 3D bioprinting. Maintaining high cell viability throughout the bioprinting process is crucial for the success of this technology, as it directly affects the accuracy of the 3D bioprinted models, the validity of experimental results, and the discovery of new therapeutic approaches. Therefore
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Hydrodynamic shear stress’ impact on mammalian cell properties and its applications in 3D bioprinting Biofabrication (IF 9.0) Pub Date : 2024-02-15 Yani Zhang, Aidan O’Mahony, Yong He, Tracie Barber
As an effective cell assembly method, three-dimensional bioprinting has been widely used in building organ models and tissue repair over the past decade. However, different shear stresses induced throughout the entire printing process can cause complex impacts on cell integrity, including reducing cell viability, provoking morphological changes and altering cellular functionalities. The potential effects
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Directing ligament-mimetic bi-directional cell organization in scaffolds through zone-specific microarchitecture for ligament tissue engineering Biofabrication (IF 9.0) Pub Date : 2024-02-14 M Sriram, Smriti Priya, Aman Mahajan, Dhirendra S Katti
Ligament tissues exhibit zone-specific anisotropic cell organization. The cells in ligament-proper are longitudinally oriented, whereas, the cells in epiligament are circumferentially oriented. Therefore, scaffolds developed to regenerate ligament tissues should possess adequate architectural features to govern ligament-mimetic bi-directional cell organization. The scaffold architectural features along
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BMP-2-immobilized PCL 3D printing scaffold with a leaf-stacked structure as a physically and biologically activated bone graft Biofabrication (IF 9.0) Pub Date : 2024-02-09 Min Ji Kim, Jin-Ho Park, Ji Min Seok, Jiwoon Jung, Tae Sung Hwang, Hee-Chun Lee, Jin Ho Lee, Su A Park, June-Ho Byun, Se Heang Oh
Although three-dimensional (3D) printing techniques are used to mimic macro- and micro-structures as well as multi-structural human tissues in tissue engineering, efficient target tissue regeneration requires bioactive 3D printing scaffolds. In this study, we developed a bone morphogenetic protein-2 (BMP-2)-immobilized polycaprolactone (PCL) 3D printing scaffold with leaf-stacked structure (LSS) (3D-PLSS-BMP)
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4D printed shape-shifting biomaterials for tissue engineering and regenerative medicine applications Biofabrication (IF 9.0) Pub Date : 2024-02-09 Maria Kalogeropoulou, Pedro J Díaz-Payno, Mohammad J Mirzaali, Gerjo J V M van Osch, Lidy E Fratila-Apachitei, Amir A Zadpoor
The existing 3D printing methods exhibit certain fabrication-dependent limitations for printing curved constructs that are relevant for many tissues. Four-dimensional (4D) printing is an emerging technology that is expected to revolutionize the field of tissue engineering and regenerative medicine (TERM). 4D printing is based on 3D printing, featuring the introduction of time as the fourth dimension
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Cancer-mediated axonal guidance of sensory neurons in a microelectrode-based innervation MPS Biofabrication (IF 9.0) Pub Date : 2024-02-09 Matthijs van der Moolen, Andrea Lovera, Fulya Ersoy, Sacha Mommo, Peter Loskill, Paolo Cesare
Despite recent advances in the field of microphysiological systems (MPSs), availability of models capable of mimicking the interactions between the nervous system and innervated tissues is still limited. This represents a significant challenge in identifying the underlying processes of various pathological conditions, including neuropathic, cardiovascular and metabolic disorders. In this novel study
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UV laser-processed microstructure for building biohybrid actuators with anisotropic movement Biofabrication (IF 9.0) Pub Date : 2024-02-09 Hiroaki Mita, Yosuke Mizuno, Hiroto Tanaka, Toshinori Fujie
Fabrication of a biohybrid actuator requires muscle cells anisotropically aligned in a line, curve, or combination of lines and curves (similar to the microstructure of living muscle tissue) to replicate lifelike movements, in addition to considering the arrangement of skeletal structure or muscular structure with anisotropic straight patterns. Here, we report a UV laser-processed microstructure for
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Multilayer nanodrug delivery system with spatiotemporal drug release improves tumor microenvironment for synergistic anticancer therapy Biofabrication (IF 9.0) Pub Date : 2024-02-07 Jiahui Cai, Yibo Yang, Jia Zhang, Zhimin Bai, Xin Zhang, Kun Li, Ming Shi, Zhiwei Liu, Liming Gao, Jidong Wang, Jian Li
The inflammatory response is one of the general symptoms that accompany tumorigenesis, the pro-inflammatory factors cyclooxygenase-2 (COX-2) and COX-2-derived prostaglandin-2 (PGE-2) in the inflammatory environment surrounding tumors possess promoting tumor development, metastasis and angiogenesis effects. In addition, the hypoxic environment of tumors severely limits the effectiveness of photodynamic
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A microscale 3D organ on a chip for recapitulating reciprocal neuroendocrine crosstalk between the hypothalamus and the pituitary gland Biofabrication (IF 9.0) Pub Date : 2024-02-06 Se-Ra Park, Myung Geun Kook, Soo-Rim Kim, Jin Woo Lee, Young Soo Yu, Chan Hum Park, Soyi Lim, Byung-Chul Oh, YunJae Jung, In-Sun Hong
Conventional 2D or even recently developed 3D in vitro culture models for hypothalamus and pituitary gland cannot successfully recapitulate reciprocal neuroendocrine communications between these two pivotal neuroendocrine tissues known to play an essential role in controlling the body’s endocrine system, survival, and reproduction. In addition, most current vitro culture models for neuroendocrine tissues
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Enhancing quality control in bioprinting through machine learning Biofabrication (IF 9.0) Pub Date : 2024-02-02 Amedeo Franco Bonatti, Giovanni Vozzi, Carmelo De Maria
Bioprinting technologies have been extensively studied in literature to fabricate three-dimensional constructs for tissue engineering applications. However, very few examples are currently available on clinical trials using bioprinted products, due to a combination of technological challenges (i.e. difficulties in replicating the native tissue complexity, long printing times, limited choice of printable
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Cation-crosslinked κ-carrageenan sub-microgel medium for high-quality embedded bioprinting Biofabrication (IF 9.0) Pub Date : 2024-02-01 Hua Zhang, Yang Luo, Zeming Hu, Mengxi Chen, Shang Chen, Yudong Yao, Jie Yao, Xiaoqi Shao, Kerong Wu, Yabin Zhu, Jun Fu
Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as a promising strategy for creating intricate biomimetic scaffolds. However, it remains a great challenge to construct tissue-scale structures with high resolution by using embedded 3D bioprinting due to the large particle size and polydispersity of the microgel medium, as well as its limited cytocompatibility. To address
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Enhancement of lacrimal gland cell function by decellularized lacrimal gland derived hydrogel Biofabrication (IF 9.0) Pub Date : 2024-02-01 Katharina E Wiebe-Ben Zakour, Sema Kaya, Julia C Matros, Michael C Hacker, Amina Cheikh-Rouhou, Kristina Spaniol, Gerd Geerling, Joana Witt
Sustainable treatment of aqueous deficient dry eye (ADDE) represents an unmet medical need and therefore requires new curative and regenerative approaches based on appropriate in vitro models. Tissue specific hydrogels retain the individual biochemical composition of the extracellular matrix and thus promote the inherent cell´s physiological function. Hence, we created a decellularized lacrimal gland
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3D-printed bioink loading with stem cells and cellular vesicles for periodontitis-derived bone defect repair Biofabrication (IF 9.0) Pub Date : 2024-01-31 Guang-Tao Yu, Wen-Xiang Zhu, Yu-Yue Zhao, Hao Cui, Hao Chen, Yan Chen, Ting-Ting Ning, Ming-Deng Rong, Lang Rao, Dan-Dan Ma
The suitable microenvironment of bone regeneration is critically important for periodontitis-derived bone defect repair. Three major challenges in achieving a robust osteogenic reaction are the exist of oral inflammation, pathogenic bacteria invasion and unaffluent seed cells. Herein, a customizable and multifunctional 3D-printing module was designed with glycidyl methacrylate (GMA) modified epsilon-poly-L-lysine
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Bioassembly of multicellular spheroids to mimic complex tissue structure using surface-modified magnetized nanofibers Biofabrication (IF 9.0) Pub Date : 2024-01-22 Hayeon Byun, Sangmin Lee, Heungsoo Shin
Advancements in biofabrication have led to major strides toward creating authentic organ models; however, replicating intricate organ structures without scaffolds remains challenging. In this study, we introduce a method utilizing surface-modifiable magnetic nanofibers to achieve precise control over spheroid functions and geometrical features, allowing the creation of multiple functional domains within
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Development of hybrid 3D printing approach for fabrication of high-strength hydroxyapatite bioscaffold using FDM and DLP techniques Biofabrication (IF 9.0) Pub Date : 2024-01-16 Yu-Jui Cheng, Tsung-Han Wu, Yu-Sheng Tseng, Wen-Fan Chen
This study develops a hybrid 3D printing approach that combines fused deposition modeling (FDM) and digital light processing (DLP) techniques for fabricating bioscaffolds, enabling rapid mass production. The FDM technique fabricates outer molds, while DLP prints struts for creating penetrating channels. By combining these components, hydroxyapatite (HA) bioscaffolds with different channel sizes (600
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Ionically annealed zwitterionic microgels for bioprinting of cartilaginous constructs Biofabrication (IF 9.0) Pub Date : 2024-01-16 František Surman, Maryam Asadikorayem, Patrick Weber, Daniel Weber, Marcy Zenobi-Wong
Foreign body response (FBR) is a pervasive problem for biomaterials used in tissue engineering. Zwitterionic hydrogels have emerged as an effective solution to this problem, due to their ultra-low fouling properties, which enable them to effectively inhibit FBR in vivo. However, no versatile zwitterionic bioink that allows for high resolution extrusion bioprinting of tissue implants has thus far been
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Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate Biofabrication (IF 9.0) Pub Date : 2024-01-16 Prabha Acharya, Pranav Joshi, Sunil Shrestha, Na Young Choi, Sehoon Jeong, Moo-Yeal Lee
Human induced pluripotent stem cell (iPSC)-derived brain organoids have potential to recapitulate the earliest stages of brain development, serving as an effective in vitro model for studying both normal brain development and disorders. However, current brain organoid culture methods face several challenges, including low throughput, high variability in organoid generation, and time-consuming, multiple
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Proteolytic remodeling of 3D bioprinted tumor microenvironments Biofabrication (IF 9.0) Pub Date : 2024-01-12 Fatemeh Rasti Boroojeni, Sajjad Naeimipour, Philip Lifwergren, Annelie Abrahamsson, Charlotta Dabrosin, Robert Selegård, Daniel Aili
In native tissue, remodeling of the pericellular space is essential for cellular activities and is mediated by tightly regulated proteases. Protease activity is dysregulated in many diseases, including many forms of cancer. Increased proteolytic activity is directly linked to tumor invasion into stroma, metastasis, and angiogenesis as well as all other hallmarks of cancer. Here we show a strategy for
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A vascularized 3D model of the human pancreatic islet for ex vivo study of immune cell-islet interaction Biofabrication (IF 9.0) Pub Date : 2024-01-11 R Hugh F Bender, Benjamen T O’Donnell, Bhupinder Shergill, Brittany Q Pham, Sima Tahmouresie, Celeste N Sanchez, Damie J Juat, Michaela M S Hatch, Venktesh S Shirure, Matthew Wortham, Kim-Vy Nguyen-Ngoc, Yesl Jun, Roberto Gaetani, Karen L Christman, Luc Teyton, Steven C George, Maike Sander, Christopher C W Hughes
Insulin is an essential regulator of blood glucose homeostasis that is produced exclusively by β cells within the pancreatic islets of healthy individuals. In those affected by diabetes, immune inflammation, damage, and destruction of islet β cells leads to insulin deficiency and hyperglycemia. Current efforts to understand the mechanisms underlying β cell damage in diabetes rely on in vitro-cultured
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Micro–nano hierarchical scaffold providing temporal-matched biological constraints for tendon reconstruction Biofabrication (IF 9.0) Pub Date : 2023-12-27 Xiaojing Xie, Junjie Xu, Jing Lin, Liang Chen, Danzhi Ding, Yage Hu, Kang Han, Chaojing Li, Fujun Wang, Jinzhong Zhao, Lu Wang
Due to the limitations of tendon biology, high-quality tendon repair remains a clinical and scientific challenge. Here, a micro–nano hierarchical scaffold is developed to promote orderly tendon regeneration by providing temporal-matched biological constraints. In short, fibrin (Fb), which provides biological constraints, is loaded into poly (DL-lactide-co-glycolide) nanoyarns with suitable degradation
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Highly compliant biomimetic scaffolds for small diameter tissue-engineered vascular grafts (TEVGs) produced via melt electrowriting (MEW) Biofabrication (IF 9.0) Pub Date : 2023-12-12 Angus Weekes, Gabrielle Wehr, Nigel Pinto, Jason Jenkins, Zhiyong Li, Christoph Meinert, Travis J Klein
Biofabrication approaches toward the development of tissue-engineered vascular grafts (TEVGs) have been widely investigated. However, successful translation has been limited to large diameter applications, with small diameter grafts frequently failing due to poor mechanical performance, in particular mismatched radial compliance. Herein, melt electrowriting (MEW) of poly(ϵ-caprolactone) has enabled
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Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness Biofabrication (IF 9.0) Pub Date : 2023-12-11 Seungmi Ryu, Claire Weber, Pei-Hsuan Chu, Ben Ernest, Vukasin M Jovanovic, Tao Deng, Jaroslav Slamecka, Hyenjong Hong, Yogita Jethmalani, Hannah M Baskir, Jason Inman, John Braisted, Marissa B Hirst, Anton Simeonov, Ty C Voss, Carlos A Tristan, Ilyas Singeç
Embryoid bodies (EBs) and self-organizing organoids derived from human pluripotent stem cells (hPSCs) recapitulate tissue development in a dish and hold great promise for disease modeling and drug development. However, current protocols are hampered by cellular stress and apoptosis during cell aggregation, resulting in variability and impaired cell differentiation. Here, we demonstrate that EBs and
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Microfluidic-based platforms for cell-to-cell communication studies Biofabrication (IF 9.0) Pub Date : 2023-12-07 Lvyang Zhu, Qu Tang, Zhenzhen Mao, Huanhuan Chen, Li Wu, Yuling Qin
Intercellular communication is critical to the understanding of human health and disease progression. However, compared to traditional methods with inefficient analysis, microfluidic co-culture technologies developed for cell–cell communication research can reliably analyze crucial biological processes, such as cell signaling, and monitor dynamic intercellular interactions under reproducible physiological
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Advances in volumetric bioprinting Biofabrication (IF 9.0) Pub Date : 2023-11-28 Sibo Jing, Liming Lian, Yingying Hou, Zeqing Li, Zihao Zheng, Gang Li, Guosheng Tang, Guoxi Xie, Maobin Xie
The three-dimensional (3D) bioprinting technologies are suitable for biomedical applications owing to their ability to manufacture complex and high-precision tissue constructs. However, the slow printing speed of current layer-by-layer (bio)printing modality is the major limitation in biofabrication field. To overcome this issue, volumetric bioprinting (VBP) is developed. VBP changes the layer-wise
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Effects of weaving parameters on the properties of completely biological tissue-engineered vascular grafts Biofabrication (IF 9.0) Pub Date : 2023-11-24 Gaëtan Roudier, Marie Hourques, Nicolas Da Silva, Maude Gluais, Emmanuel Binyet, Jean-Marc Olive, Nicolas L’Heureux
Tissue-engineered vascular grafts (TEVGs) made of human textiles have been recently introduced and offer remarkable biocompatibility as well as tunable mechanical properties. The approach combines the use of cell-assembled extracellular matrix (CAM) threads, produced by cultured cells in vitro, with weaving, a versatile assembly method that gives fine control over graft properties. Herein, we investigated
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Modular 3D printed platform for fluidically connected human brain organoid culture Biofabrication (IF 9.0) Pub Date : 2023-11-20 Babak Rezaei, Jessica Giacomoni, Fredrik Nilsson, Edoardo Sozzi, Alessandro Fiorenzano, Malin Parmar, Stephan S Keller, Janko Kajtez
Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely
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Synergistic coupling between 3D bioprinting and vascularization strategies Biofabrication (IF 9.0) Pub Date : 2023-11-20 Miji Yeo, Anwita Sarkar, Yogendra Pratap Singh, Irem Deniz Derman, Pallab Datta, Ibrahim T Ozbolat
Three-dimensional (3D) bioprinting offers promising solutions to the complex challenge of vascularization in biofabrication, thereby enhancing the prospects for clinical translation of engineered tissues and organs. While existing reviews have touched upon 3D bioprinting in vascularized tissue contexts, the current review offers a more holistic perspective, encompassing recent technical advancements
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Bioprinting of scaled-up meniscal grafts by spatially patterning phenotypically distinct meniscus progenitor cells within melt electrowritten scaffolds Biofabrication (IF 9.0) Pub Date : 2023-11-17 Xavier Barceló, Kian Eichholz, Inês Gonçalves, Gabriela S Kronemberger, Alexandre Dufour, Orquidea Garcia, Daniel J Kelly
Meniscus injuries are a common problem in orthopedic medicine and are associated with a significantly increased risk of developing osteoarthritis. While developments have been made in the field of meniscus regeneration, the engineering of cell-laden constructs that mimic the complex structure, composition and biomechanics of the native tissue remains a significant challenge. This can be linked to the
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Micropatterning the organization of multicellular structures in 3D biological hydrogels; insights into collective cellular mechanical interactions Biofabrication (IF 9.0) Pub Date : 2023-11-16 Bar Ergaz, Shahar Goren, Ayelet Lesman
Control over the organization of cells at the microscale level within supporting biomaterials can push forward the construction of complex tissue architectures for tissue engineering applications and enable fundamental studies of how tissue structure relates to its function. While cells patterning on 2D substrates is a relatively established and available procedure, micropatterning cells in biomimetic
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Long-term in vitro culture of 3D brain tissue model based on chitosan thermogel Biofabrication (IF 9.0) Pub Date : 2023-11-10 Donatella Di Lisa, Lorenzo Muzzi, Alberto Lagazzo, Andrea Andolfi, Sergio Martinoia, Laura Pastorino
Methods for studying brain function and disease heavily rely on in vivo animal models, ex-vivo tissue slices, and 2D cell culture platforms. These methods all have limitations that significantly impact the clinical translatability of results. Consequently, models able to better recapitulate some aspects of in vivo human brain are needed as additional preclinical tools. In this context, 3D hydrogel-based
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A novel perfusion bioreactor promotes the expansion of pluripotent stem cells in a 3D-bioprinted tissue chamber Biofabrication (IF 9.0) Pub Date : 2023-11-10 Elizabeth R Komosa, Wei-Han Lin, Bhushan Mahadik, Marisa S Bazzi, DeWayne Townsend, John P Fisher, Brenda M Ogle
While the field of tissue engineering has progressed rapidly with the advent of 3D bioprinting and human induced pluripotent stem cells (hiPSCs), impact is limited by a lack of functional, thick tissues. One way around this limitation is to 3D bioprint tissues laden with hiPSCs. In this way, the iPSCs can proliferate to populate the thick tissue mass prior to parenchymal cell specification. Here we
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Enhancement of properties of a cell-laden GelMA hydrogel-based bioink via calcium phosphate phase transition Biofabrication (IF 9.0) Pub Date : 2023-11-07 Jueun Kim, Naren Raja, Yeong-Jin Choi, Chang-Woo Gal, Aram Sung, Honghyun Park, Hui-suk Yun
To improve the properties of the hydrogel-based bioinks, a calcium phosphate phase transition was applied, and the products were examined. We successfully enhanced the mechanical properties of the hydrogels by adding small amounts (< 0.5 wt%) of alpha-tricalcium phosphate (α-TCP) to photo-crosslinkable gelatin methacrylate (GelMA). As a result of the hydrolyzing calcium phosphate phase transition involving
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Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation Biofabrication (IF 9.0) Pub Date : 2023-11-06 Huong Thi Le, Huu Lam Phan, Andreas Lenshof, Van Thuy Duong, Cholong Choi, Chaenyung Cha, Thomas Laurell, Kyo-in Koo
Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material
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Novel 3D-printing bilayer GelMA-based hydrogel containing BP, β-TCP and exosomes for cartilage–bone integrated repair Biofabrication (IF 9.0) Pub Date : 2023-10-31 Ting Sun, Zhiqiang Feng, Wenpeng He, Chufeng Li, Songning Han, Zejian Li, Rui Guo
The integrated repair of cartilage and bone involves the migration and differentiation of cells, which has always been a difficult problem to be solved. We utilize the natural biomaterial gelatin to construct gelatin methacryloyl (GelMA), a hydrogel scaffold with high cell affinity. GelMA is mixed with different components to print a bi-layer porous hydrogel scaffold with different modulus and composition
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An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects Biofabrication (IF 9.0) Pub Date : 2023-10-27 Donagh G O’Shea, Tom Hodgkinson, Caroline M Curtin, Fergal J O’Brien
Current treatments for repairing articular cartilage defects are limited. However, pro-chondrogenic hydrogels formulated using articular cartilage matrix components (such as hyaluronic acid (HA) and collagen type II (Col II)), offer a potential solution if they could be injected into the defect via minimally invasive arthroscopic procedures, or used as bioinks to 3D print patient-specific customised
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Microfabrication and lab-on-a-chip devices promote in vitro modeling of neural interfaces for neuroscience researches and preclinical applications Biofabrication (IF 9.0) Pub Date : 2023-10-26 Yang Liu, Xiangyun Yao, Cunyi Fan, Guifeng Zhang, Xi Luo, Yun Qian
Neural tissues react to injuries through the orchestration of cellular reprogramming, generating specialized cells and activating gene expression that helps with tissue remodeling and homeostasis. Simplified biomimetic models are encouraged to amplify the physiological and morphological changes during neural regeneration at cellular and molecular levels. Recent years have witnessed growing interest
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A 3D-printed blood-brain barrier model with tunable topology and cell-matrix interactions Biofabrication (IF 9.0) Pub Date : 2023-10-20 Louis S Paone, Mohammed Mehdi Benmassaoud, Aidan Curran, Sebastián L Vega, Peter A Galie
Recent developments in digital light processing (DLP) can advance the structural and biochemical complexity of perfusable in vitro models of the blood–brain barrier. Here, we describe a strategy to functionalize complex, DLP-printed vascular models with multiple peptide motifs in a single hydrogel. Different peptides can be clicked into the walls of distinct topologies, or the peptide motifs lining
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Human vascularized bile duct-on-a chip: a multi-cellular micro-physiological system for studying cholestatic liver disease Biofabrication (IF 9.0) Pub Date : 2023-10-20 Yu Du, Iris E M de Jong, Kapish Gupta, Orit Waisbourd-Zinman, Adi Har-Zahav, Carol J Soroka, James L Boyer, Jessica Llewellyn, Chengyang Liu, Ali Naji, William J Polacheck, Rebecca G Wells
Exploring the pathogenesis of and developing therapies for cholestatic liver diseases such as primary sclerosing cholangitis (PSC) remains challenging, partly due to a paucity of in vitro models that capture the complex environments contributing to disease progression and partly due to difficulty in obtaining cholangiocytes. Here we report the development of a human vascularized bile duct-on-a-chip
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Biomimetic human skin model patterned with rete ridges Biofabrication (IF 9.0) Pub Date : 2023-10-20 Maxwell B Nagarajan, Alexander J Ainscough, Daniel S Reynolds, Sebastien G M Uzel, Jason W Bjork, Bryan A Baker, Amy K McNulty, Susan L Woulfe, Jennifer A Lewis
Rete ridges consist of undulations between the epidermis and dermis that enhance the mechanical properties and biological function of human skin. However, most human skin models are fabricated with a flat interface between the epidermal and dermal layers. Here, we report a micro-stamping method for producing human skin models patterned with rete ridges of controlled geometry. To mitigate keratinocyte-induced
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3D-printed biomimetic scaffolds with precisely controlled and tunable structures guide cell migration and promote regeneration of osteochondral defect Biofabrication (IF 9.0) Pub Date : 2023-10-18 Yuqing Gu, Yiwei Zou, Yuxuan Huang, Renjie Liang, Yicong Wu, Yifan Hu, Yi Hong, Xianzhu Zhang, Yi-Chin Toh, Hongwei Ouyang, Shufang Zhang
Untreated osteochondral defects will develop into osteoarthritis, affecting patients’ quality of life. Since articular cartilage and subchondral bone exhibit distinct biological characteristics, repairing osteochondral defects remains a major challenge. Previous studies have tried to fabricate multilayer scaffolds with traditional methods or 3D printing technology. However, the efficacy is unsatisfactory
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On the reproducibility of extrusion-based bioprinting: round robin study on standardization in the field Biofabrication (IF 9.0) Pub Date : 2023-10-11 David Grijalva Garces, Svenja Strauß, Sarah Gretzinger, Barbara Schmieg, Tomasz Jüngst, Jürgen Groll, Lorenz Meinel, Isabelle Schmidt, Hanna Hartmann, Katja Schenke-Layland, Nico Brandt, Michael Selzer, Stefan Zimmermann, Peter Koltay, Alexander Southan, Günter E M Tovar, Sarah Schmidt, Achim Weber, Tilman Ahlfeld, Michael Gelinsky, Thomas Scheibel, Rainer Detsch, Aldo R Boccaccini, Toufik Naolou,
The outcome of three-dimensional (3D) bioprinting heavily depends, amongst others, on the interaction between the developed bioink, the printing process, and the printing equipment. However, if this interplay is ensured, bioprinting promises unmatched possibilities in the health care area. To pave the way for comparing newly developed biomaterials, clinical studies, and medical applications (i.e. printed
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Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing Biofabrication (IF 9.0) Pub Date : 2023-10-10 Walaa M Metwally, Salma E El-Habashy, Lobna S El-Hosseiny, Marwa M Essawy, Hoda M Eltaher, Labiba K El-Khordagui
There is a constant demand for novel materials/biomedical devices to accelerate the healing of hard-to-heal wounds. Herein, an innovative 3D-printed bioinspired construct was developed as an antibacterial/regenerative scaffold for diabetic wound healing. Hyaluronic/chitosan (HA/CS) ink was used to fabricate a bilayer scaffold comprising a dense plain hydrogel layer topping an antibacterial/regenerative
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Translational biomaterials of four-dimensional bioprinting for tissue regeneration Biofabrication (IF 9.0) Pub Date : 2023-10-09 Leah Faber, Anne Yau, Yupeng Chen
Bioprinting is an additive manufacturing technique that combines living cells, biomaterials, and biological molecules to develop biologically functional constructs. Three-dimensional (3D) bioprinting is commonly used as an in vitro modeling system and is a more accurate representation of in vivo conditions in comparison to two-dimensional cell culture. Although 3D bioprinting has been utilized in various
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3D-bioprintable endothelial cell-laden sacrificial ink for fabrication of microvessel networks Biofabrication (IF 9.0) Pub Date : 2023-09-28 Kun-Chih Cheng, Patrick Theato, Shan-hui Hsu
Although various research efforts have been made to produce a vascular-like network structure as scaffolds for tissue engineering, there are still several limitations. Meanwhile, no articles have been published on the direct embedding of cells within a glucose sensitive sacrificial hydrogel followed by three-dimensional (3D) bioprinting to fabricate vascular structures. In this study, the hydrogel
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3D bioprinted endothelial cell-microglia coculture for diabetic retinopathy modeling Biofabrication (IF 9.0) Pub Date : 2023-09-27 Haixiang Wu, Fangcheng Xu, Yunfang Luo, Yibao Zhang, Min Tang
Diabetic retinopathy (DR) is a common diabetes complication leading to vision impairment or blindness due to retinal vasculature alterations. Hyperglycemia induces structural alterations, inflammation, and angiogenic factor upregulation. Current treatments targeting vascular endothelial growth factor are insufficient for approximately 20% of DR patients, necessitating alternative approaches. Microglia
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Mimicking sarcolemmal damage in vitro: a contractile 3D model of skeletal muscle for drug testing in Duchenne muscular dystrophy Biofabrication (IF 9.0) Pub Date : 2023-09-27 Ainoa Tejedera-Villafranca, Marisol Montolio, Javier Ramón-Azcón, Juan M Fernández-Costa
Duchenne muscular dystrophy (DMD) is the most prevalent neuromuscular disease diagnosed in childhood. It is a progressive and wasting disease, characterized by a degeneration of skeletal and cardiac muscles caused by the lack of dystrophin protein. The absence of this crucial structural protein leads to sarcolemmal fragility, resulting in muscle fiber damage during contraction. Despite ongoing efforts
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Biofabrication of an in-vitro bone model for Gaucher disease Biofabrication (IF 9.0) Pub Date : 2023-09-22 Dishary Banerjee, Margarita M Ivanova, Nazmiye Celik, Myoung Hwan Kim, Irem Deniz Derman, Renuka Pudi Limgala, Ibrahim T Ozbolat, Ozlem Goker-Alpan
Gaucher disease (GD), the most prevalent lysosomal disorder, is caused by GBA1 gene mutations, leading to deficiency of glucocerebrosidase, and accumulation of glycosphingolipids in cells of the mononuclear phagocyte system. While skeletal diseases are the leading cause of morbidity and reduced quality of life in GD, the pathophysiology of bone involvement is not yet fully understood, partly due to
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Gastrointestinal organs and organoids-on-a-chip: advances and translation into the clinics Biofabrication (IF 9.0) Pub Date : 2023-09-22 Mariana R Carvalho, Le-Ping Yan, Bo Li, Chang-Hua Zhang, Yu-Long He, Rui L Reis, Joaquim M Oliveira
Microfluidic organs and organoids-on-a-chip models of human gastrointestinal systems have been established to recreate adequate microenvironments to study physiology and pathophysiology. In the effort to find more emulating systems and less costly models for drugs screening or fundamental studies, gastrointestinal system organoids-on-a-chip have arisen as promising pre-clinical in vitro model. This
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Double-layered blood vessels over 3 mm in diameter extruded by the inverse-gravity technique Biofabrication (IF 9.0) Pub Date : 2023-09-22 Van Thuy Duong, Chanh Trung Nguyen, Huu Lam Phan, Van Phu Le, Thao Thi Dang, Cholong Choi, Jongmo Seo, Chaenyung Cha, Sung Hoon Back, Kyo-in Koo
One of the most promising techniques for treating severe peripheral artery disease is the use of cellular tissue-engineered vascular grafts (TEVGs). This study proposes an inverse-gravity (IG) extrusion technique for creating long double-layered cellular TEVGs with diameters over 3 mm. A three-layered coaxial laminar hydrogel flow in an 8 mm-diameter pipe was realised simply by changing the extrusion
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Is it possible to 3D bioprint load-bearing bone implants? A critical review Biofabrication (IF 9.0) Pub Date : 2023-09-19 Tanmay Gupta, Subrata Bandhu Ghosh, Sanchita Bandyopadhyay-Ghosh, Mohini Sain
Rehabilitative capabilities of any tissue engineered scaffold rely primarily on the triad of (i) biomechanical properties such as mechanical properties and architecture, (ii) chemical behavior such as regulation of cytokine expression, and (iii) cellular response modulation (including their recruitment and differentiation). The closer the implant can mimic the native tissue, the better it can rehabilitate
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Physically-based simulation of elastic-plastic fusion of 3D bioprinted spheroids Biofabrication (IF 9.0) Pub Date : 2023-09-15 Hassan Bahrami, Federico Sichetti, Enrico Puppo, Laura Vettori, Clara Liu Chung Ming, Stuart Perry, Carmine Gentile, Nico Pietroni
Spheroids are microtissues containing cells organized in a spherical shape whose diameter is usually less than a millimetre. Depending on the properties of the environment they are placed in, some nearby spheroids spontaneously fuse and generate a tissue. Given their potential to mimic features typical of body parts and their ability to assemble by fusing in permissive hydrogels, they have been used
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A scalable human iPSC-based neuromuscular disease model on suspended biobased elastomer nanofiber scaffolds Biofabrication (IF 9.0) Pub Date : 2023-09-05 Aimee Cheesbrough, Peter Harley, Federica Riccio, Lei Wu, Wenhui Song, Ivo Lieberam
Many devastating neuromuscular diseases currently lack effective treatments. This is in part due to a lack of drug discovery platforms capable of assessing complex human neuromuscular disease phenotypes in a scalable manner. A major obstacle has been generating scaffolds to stabilise mature contractile myofibers in a multi-well assay format amenable to high content image (HCI) analysis. This study
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Bone on-a-chip: a 3D dendritic network in a screening platform for osteocyte-targeted drugs Biofabrication (IF 9.0) Pub Date : 2023-09-01 Maria Veronica Lipreri, Gemma Di Pompo, Elisa Boanini, Gabriela Graziani, Enrico Sassoni, Nicola Baldini, Sofia Avnet
Age-related musculoskeletal disorders, including osteoporosis, are frequent and associated with long lasting morbidity, in turn significantly impacting on healthcare system sustainability. There is therefore a compelling need to develop reliable preclinical models of disease and drug screening to validate novel drugs possibly on a personalized basis, without the need of in vivo assay. In the context
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Development and characterization of biological sutures made of cell-assembled extracellular matrix Biofabrication (IF 9.0) Pub Date : 2023-08-31 Paul Borchiellini, Adeline Rames, François Roubertie, Nicolas L’Heureux, Fabien Kawecki
Most vascular surgical repair procedures, such as vessel anastomoses, requires using suture materials that are mechanically efficient and accepted by the patient’s body. These materials are essentially composed of synthetic polymers, such as polypropylene (ProleneTM) or polyglactin (VicrylTM). However, once implanted in patients, they are recognized as foreign bodies, and the patient’s immune system
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3D vascularized microphysiological system for investigation of tumor-endothelial crosstalk in anti-cancer drug resistance Biofabrication (IF 9.0) Pub Date : 2023-08-24 Seunggyu Kim, Joonha Park, Jin-Nyoung Ho, Danhyo Kim, Sangchul Lee, Jessie S Jeon
Despite the advantages of microfluidic system in drug screening, vascular systems responsible for the transport of drugs and nutrients have been hardly considered in the microfluidic-based chemotherapeutic screening. Considering the physiological characteristics of highly vascularized urinary tumors, we here investigated the chemotherapeutic response of bladder tumor cells using a vascularized tumor
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Sequentially suspended 3D bioprinting of multiple-layered vascular models with tunable geometries for in vitro modeling of arterial disorders initiation Biofabrication (IF 9.0) Pub Date : 2023-08-24 Chen Pan, Jingwen Xu, Qiqi Gao, Wei Li, Tao Sun, Jiping Lu, Qing Shi, Yafeng Han, Ge Gao, Jinhua Li
As the main precursor of arterial disorders, endothelial dysfunction preferentially occurs in regions of arteries prone to generating turbulent flow, particularly in branched regions of vasculatures. Although various diseased models have been engineered to investigate arterial pathology, producing a multiple-layered vascular model with branched geometries that can recapitulate the critical physiological