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Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells BMC Biotechnol. (IF 3.5) Pub Date : 2024-03-15 Mona Elradi, Ahmed I. Ahmed, Ahmed M. Saleh, Khaled M. A. Abdel-Raouf, Lina Berika, Yara Daoud, Asma Amleh
Cancer associated drug resistance is a major cause for cancer aggravation, particularly as conventional therapies have presented limited efficiency, low specificity, resulting in long term deleterious side effects. Peptide based drugs have emerged as potential alternative cancer treatment tools due to their selectivity, ease of design and synthesis, safety profile, and low cost of manufacturing. In
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Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice BMC Biotechnol. (IF 3.5) Pub Date : 2024-03-08 Qing Wang, Zhiyi He, Jinqi Zhu, Mengyun Hu, Liu Yang, Hongzhong Yang
Smoking was a major risk factor for chronic obstructive pulmonary disease (COPD). This study plan to explore the mechanism of Polyphyllin B in lung injury induced by cigarette smoke (CSE) in COPD. Network pharmacology and molecular docking were applied to analyze the potential binding targets for Polyphyllin B and COPD. Commercial unfiltered CSE and LPS were used to construct BEAS-2B cell injury in
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Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry BMC Biotechnol. (IF 3.5) Pub Date : 2024-03-07 Daguang Wang, Junshan Wang, Xinghong Liu, Kehe Du, Hongjun Liu, Xiaofeng Yang, Tianyi Liu, Qian Liu, Meng Wang, Jian Guo
The objective of this study was to establish a methodology for determining carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) concentrations in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The test results were also used for clinical aging research. Human plasma samples were incubated with aqueous perfluorovaleric acid (NFPA), succeeded by precipitation utilizing
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Iron/Copper/Phosphate nanocomposite as antimicrobial, antisnail, and wheat growth-promoting agent BMC Biotechnol. (IF 3.5) Pub Date : 2024-03-05 Nashwa H. Abdullah, Nouran A. Elbialy, Mohamed Abdelnaser Amer, Mostafa Kh. Gabr, Amira Salah El-Din Youssef, Mohamed H. Sharaf, M. E. Shehata, Mohamed H. Kalaba, Elham R. S. Soliman
One of the current challenges is to secure wheat crop production to meet the increasing global food demand and to face the increase in its purchasing power. Therefore, the current study aimed to exploit a new synthesized nanocomposite to enhance wheat growth under both normal and drought regime. The effectiveness of this nanocomposite in improving the microbiological quality of irrigation water and
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Staphopain mediated virulence and antibiotic resistance alteration in co-infection of Staphylococcus aureus and Pseudomonas aeruginosa: an animal model BMC Biotechnol. (IF 3.5) Pub Date : 2024-03-04 Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Yousef Alikhani, Mohammad-Ali Shahbazi, Mohammad Reza Arabestani
Polymicrobial communities lead to worsen the wound infections, due to mixed biofilms, increased antibiotic resistance, and altered virulence production. Promising approaches, including enzymes, may overcome the complicated condition of polymicrobial infections. Therefore, this study aimed to investigate Staphopain A-mediated virulence and resistance alteration in an animal model of Staphylococcus aureus
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Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities BMC Biotechnol. (IF 3.5) Pub Date : 2024-02-08 Tetiana Krupodorova, Victor Barshteyn, Victoria Tsygankova, Mustafa Sevindik, Yaroslav Blume
The production of Pleurotus ostreatus mycelium as a promising object for use in food and other industries is hampered by a lack of information about the strain-specificity of this fungus mycelium growth and its acquisition of various biological activities. Therefore, this research aimed to investigate mycelial growth of different P. ostreatus strains on varies solid and liquid media as well as to evaluate
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Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis BMC Biotechnol. (IF 3.5) Pub Date : 2024-02-06 Mohamed T. Shaaban, Briksam S. Mohamed, Muhammad Zayed, Sabha M. El-Sabbagh
Silver nanoparticles (Ag-NPs) have a unique mode of action as antibacterial agents in addition to their anticancer and antioxidant properties. In this study, microbial nanotechnology is employed to synthesize Ag-NPs using the cell filtrate of Streptomyces enissocaesilis BS1. The synthesized Ag-NPs are confirmed by ultraviolet-visible (UV-Vis), Fourier transform infrared (FT-IR), X-ray diffraction (XRD)
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Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata BMC Biotechnol. (IF 3.5) Pub Date : 2024-02-01 Germano Sollazzo, Katerina Nikolouli, Georgia Gouvi, Roswitha A. Aumann, Marc F. Schetelig, Kostas Bourtzis
The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT programs, which can be achieved through the development of genetic sexing strains (GSS). The most successful GSS developed to date
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Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2 BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-25 Manal S. Selim, Marwa M. Mounier, Sayeda A. Abdelhamid, Ahmed Abdelghani Hamed, Mostafa M. Abo Elsoud, Sahar S. Mohamed
L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our study is based on isolating, purifying, and screening the marine bacteria that can produce arginase. The highest arginase producing bacteria will be identified by using microbiological and molecular
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Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-23 Samira Asefian, Mansureh Ghavam
Recently there have been a variety of methods to synthesize silver nanoparticles, among which the biosynthesis method is more noticeable due to features like being eco-friendly, simple, and cost-efficient. The present study aims for the green synthesis of silver nanoparticles from the extract of the three plants A. wilhelmsi, M. chamomilla, and C. longa; moreover, it pledges to measure the antibacterial
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Reaping the benefits of liquid handlers for high-throughput gene expression profiling in a marine model invertebrate BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-19 Giovanni Annona, Assunta Liberti, Carla Pollastro, Antonietta Spagnuolo, Paolo Sordino, Pasquale De Luca
Modern high-throughput technologies enable the processing of a large number of samples simultaneously, while also providing rapid and accurate procedures. In recent years, automated liquid handling workstations have emerged as an established technology for reproducible sample preparation. They offer flexibility, making them suitable for an expanding range of applications. Commonly, such approaches
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Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21 BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-17 Ahmed A. Hamed, Mosad A. Ghareeb, Ayda K. Kelany, Mohamed Abdelraof, Hoda A. Kabary, Nariman R. Soliman, Mohamed E. Elawady
The growing spread of infectious diseases has become a potential global health threat to human beings. According to WHO reports, in this study, we investigated the impact of co-cultivating the isolated endophytic fungus Aspergillus sp. CO2 and Bacillus sp. COBZ21 as a method to stimulate the production of natural bioactive substances. (GC/MS)-based metabolomics profiling of two sponge-associated microbes
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A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-10 Meijuan Zhang, Xiaoping Fu, Rongrong Gu, Bohua Zhao, Xingya Zhao, Hui Song, Hongchen Zheng, Jianyong Xu, Wenqin Bai
Lytic polysaccharide monooxygenases (LPMOs) catalyzing the oxidative cleavage of different types of polysaccharides have potential to be used in various industries. However, AA13 family LPMOs which specifically catalyze starch substrates have relatively less members than AA9 and AA10 families to limit their application range. Amylase has been used in enzymatic desizing treatment of cotton fabric for
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Tuning spacer length improves the functionality of the nanobody-based VEGFR2 CAR T cell BMC Biotechnol. (IF 3.5) Pub Date : 2024-01-04 Fatemeh Hajari Taheri, Mahmoud Hassani, Zahra Sharifzadeh, Mahdi Behdani, Shahryar Abdoli, Mahtab Sayadi, Kowsar Bagherzadeh, Arash Arashkia, Mohsen Abolhassani
The chimeric antigen receptor-expressing T (CAR-T) cells for cancer immunotherapy have obtained considerable clinical importance. CAR T cells need an optimized intracellular signaling domain to get appropriately activated and also for the proper antigen recognition, the length and composition of the extracellular spacer are critical factors. We constructed two third-generation nanobody-based VEGFR2-CARs
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Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine BMC Biotechnol. (IF 3.5) Pub Date : 2023-12-19 Akram Firouzi Amandi, Seyed Abbas Shahrtash, Shaylan Kalavi, Afshin Moliani, Hanieh Mousazadeh, Mehdi Rezai Seghin Sara, Mehdi Dadashpour
In tissue engineering (TE) and regenerative medicine, the accessibility of engineered scaffolds that modulate inflammatory states is extremely necessary. The aim of the current work was to assess the efficacy of metformin (MET) incorporated in PLGA/Collagen nanofibers (Met-PLGA/Col NFs) to modulate RAW264.7 macrophage phenotype from pro-inflammatory status (M1) to anti-inflammatory status (M2). Given
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Production of a potential multistrain probiotic in co-culture conditions using agro-industrial by-products-based medium for fish nutrition BMC Biotechnol. (IF 3.5) Pub Date : 2023-12-15 Marcelo Fernando Valle-Vargas, Ruth Yolanda Ruiz-Pardo, Luisa Villamil-Díaz, María Ximena Quintanilla-Carvajal
Probiotics are viable microorganisms that when administered in adequate amounts confer health benefits to the host. In fish, probiotic administration has improved growth, and immunological parameters. For this reason, it is necessary production of probiotic bacteria, however, commercial culture mediums used for probiotic growth are expensive, so the design of a “low” cost culture medium is necessary
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Research on the targeted improvement of the yield of a new VB12-producing strain, Ensifer adhaerens S305, based on genomic and transcriptomic analysis BMC Biotechnol. (IF 3.5) Pub Date : 2023-12-11 Yongheng Liu, Wei Huang, Qi Wang, Cilang Ma, Yongyong Chang, Jianyu Su
Vitamin B12 (VB12) has a wide range of applications and high economic value. In this study, a new strain with high VB12 production potential, Ensifer adhaerens S305, was identified in sewage. Because E. adhaerens strains have become the main strains for VB12 production via fermentation in recent years, the directional modification of the S305 strain to obtain a strain suitable for the industrial production
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Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+ BMC Biotechnol. (IF 3.5) Pub Date : 2023-12-08 H. M. L. P. B. Herath, W. R. M. de Silva, R. S. Dassanayake, Y. I. N. S. Gunawardene, J. R. P. Jayasingha, M. K. Gayashan, L. O. B. Afonso, K. M. N. de Silva
In this study, we designed a novel genetic circuit sensitive to Cd2+, Zn2+ and Pb2+ by mimicking the CadA/CadR operon system mediated heavy metal homeostasis mechanism of Pseudomonas aeruginosa. The regular DNA motifs on natural operon were reconfigured and coupled with the enhanced Green Fluorescent Protein (eGFP) reporter to develop a novel basic NOT type logic gate CadA/CadR-eGFP to respond metal
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Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach BMC Biotechnol. (IF 3.5) Pub Date : 2023-12-04 Santosh Thapa, Suping Zhou, Joshua O’Hair, Kamal Al Nasr, Alexander Ropelewski, Hui Li
Goat rumen microbial communities are perceived as one of the most potential biochemical reservoirs of multi-functional enzymes, which are applicable to enhance wide array of bioprocesses such as the hydrolysis of cellulose and hemi-cellulose into fermentable sugar for biofuel and other value-added biochemical production. Even though, the limited understanding of rumen microbial genetic diversity and
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The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa BMC Biotechnol. (IF 3.5) Pub Date : 2023-11-29 Fanglei Xue, Zhen Zhao, Shuying Gu, Meixin Chen, Jing Xu, Xuegang Luo, Jingen Li, Chaoguang Tian
Filamentous fungi are efficient degraders of plant biomass and the primary producers of commercial cellulolytic enzymes. While the transcriptional regulation mechanisms of cellulases have been continuously explored in lignocellulolytic fungi, the induction of cellulase production remains a complex multifactorial system, with several aspects still largely elusive. In this study, we identified a Zn2Cys6
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An online soft sensor method for biochemical reaction process based on JS-ISSA-XGBoost BMC Biotechnol. (IF 3.5) Pub Date : 2023-11-08 Ligang Zhang, Bo Wang, Yao Shen, Yongxin Nie
A method combining offline techniques and the just-in-time learning strategy (JITL) is proposed, because the biochemical reaction process often encounters changing features and parameters over time. Firstly, multiple sub-databases in the fermentation process are constructed offline by an improved fuzzy C-means algorithm and the sample data are adaptively pruned by a similarity query threshold. Secondly
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Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application BMC Biotechnol. (IF 3.5) Pub Date : 2023-11-03 Xinran Duan, Wei Zhai, Xintian Li, Sicheng Wu, Ye Wang, Lixia Wang, Wangdui Basang, Yanbin Zhu, Yunhang Gao
A lot of kitchen waste oil is produced every day worldwide, leading to serious environmental pollution. As one of the environmental protection methods, microorganisms are widely used treating of various wastes. Lipase, as one of the cleaning agents can effectively degrade kitchen waste oil. The composting process of pig carcasses produces many lipase producing microorganisms, rendering compost products
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Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers BMC Biotechnol. (IF 3.5) Pub Date : 2023-10-31 Zhao Jianbo, Wei Jun, Wang Xuanlin, Cao Hui
γ-polyglutamic acid (γ-PGA) is a biomarker that can be directly obtained by microbial fermentation. Poly(amino acid) superabsorbent polymers (SAPs) were prepared with purified γ-PGA as raw material and ethylene glycol diglycidyl ether (EGDGE) as a cross-linking agent. However, γ-PGA fermentation broth has a high viscosity, requires complex extraction and separation processes, and entails high energy
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Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen BMC Biotechnol. (IF 3.5) Pub Date : 2023-10-24 M. Golchin, S. Mollayi, E. Mohammadi, N. Eskandarzade
Brucellosis remains one of the global health concerns that reemerges in recent years. Delayed or inaccurate diagnosis end to a long treatment duration and financial burden; therefore, finding a good antigen for detection of specific anti-Brucella antibodies is crucial. We intended to evaluate the serodiagnosis value of recombinant Brucella outer membrane protein 19 kDa (rOMP19) using indirect ELISA
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A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum BMC Biotechnol. (IF 3.5) Pub Date : 2023-10-11 Jian He, Ping Liang, Tingting Wang, Shuang Han
A chemiluminescent immunoassay for human serum Cystatin C (Cys C) was established using a direct-antibody sandwich model. The immunoassay kit uses magnetic separation technology, using magnetic particles as the reaction solid phase, alkaline phosphatase as the marker enzyme, and a new chemiluminescent substrate APLS as the substrate. It has the characteristics of high sensitivity and short reaction
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Target identification of small molecules: an overview of the current applications in drug discovery BMC Biotechnol. (IF 3.5) Pub Date : 2023-10-10 Yasser Tabana, Dinesh Babu, Richard Fahlman, Arno G. Siraki, Khaled Barakat
Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification research can be challenging, two main approaches can help researchers make significant discoveries: affinity-based pull-down and label-free methods. Affinity-based pull-down methods use small molecules
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Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model BMC Biotechnol. (IF 3.5) Pub Date : 2023-10-03 Lu Shi, Tiantian Zhao, Lei Huang, Xiaomin Pan, Tianzhen Wu, Xin Feng, Taoli Chen, Jiamin Wu, Jianlou Niu
The major safety concern of the clinical application of wild type FGF19 (FGF19WT) emerges given that its extended treatment causes hepatocellular carcinoma. Therefore, we previously generated a safer FGF19 variant - FGF19ΔKLB, which have same effects on glycemic control and bile acid production but much less mitogenic activity. However, it remains unclear as to whether FGF19ΔKLB ameliorates intrahepatic
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Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-27 Zahra Ahmadzadeh Chaleshtori, Ali Asghar Rastegari, Hashem Nayeri, Abbas Doosti
Helicobacter pylori cause a variety of gastric malignancies, gastric ulcers, and cause erosive diseases. The extreme nature of the bacterium and the implantation of this bacterium protects it against designing a potent drug against it. Therefore, employing a precise and effective design for a more safe and stable antigenic vaccine against this pathogen can effectively control its associated infections
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Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-27 Bahareh Nowruzi, Bilal Saad Jalil, James S Metcalf
Metal nanoparticles exhibit excellent antifungal abilities and are seen as a good substitute for controlling different kinds of fungi. Of all known taxa, cyanobacteria have received significant consideration as nanobiofactories, as a result of the cellular assimilation of heavy metals from the environment. The cellular bioactive enzymes, polysaccharides and pigments can be used as reducers and coatings
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Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and a novel antibiotic resistance marker BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-18 Nikolaus I. Stellner, Zora S. Rerop, Norbert Mehlmer, Mahmoud Masri, Marion Ringel, Thomas B. Brück
Cutaneotrichosporon oleaginosus is an oleaginous yeast that can produce up to 80% lipid per dry weight. Its high capacity for the biosynthesis of single cell oil makes it highly interesting for the production of engineered lipids or oleochemicals for industrial applications. However, the genetic toolbox for metabolic engineering of this non-conventional yeast has not yet been systematically expanded
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Effect of poly (lactic-co-glycolic acid) polymer nanoparticles loaded with vancomycin against Staphylococcus aureus biofilm BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-18 Ellahe Nouruzi, Seyed Mostafa Hosseini, Babak Asghari, Reza Mahjoub, Ehsan Nazarzadeh Zare, Mohammad-Ali Shahbazi, Fereshte Kalhori, Mohammad Reza Arabestani
Staphylococcus aureus is a unique challenge for the healthcare system because it can form biofilms, is resistant to the host's immune system, and is resistant to numerous antimicrobial therapies. The aim of this study was to investigate the effect of poly (lactic-co-glycolic acid) (PLGA) polymer nanoparticles loaded with vancomycin and conjugated with lysostaphin (PLGA-VAN-LYS) on inhibiting S. aureus
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A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-14 Huiming Jiang, Jun Lu, Jiawei Li, Zizheng Liu, Fufei Chen, Rui Wu, Xingquan Xu, Yuan Liu, Yiqiu Jiang, Dongquan Shi
Cartilage defects are common sports injuries without significant treatment. Articular cartilage with inferior regenerative potential resulted in the poor formation of hyaline cartilage in defects. Acellular matrix scaffolds provide a microenvironment and biochemical properties similar to those of native tissues and are widely used for tissue regeneration. Therefore, we aimed to design a novel acellular
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Development of short hairpin RNA expression vectors targeting the internal ribosomal entry site of the classical swine fever virus genomic RNA BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-08 Riai Okamoto, Nobumasa Ito, Yutaro Ide, Bouchra Kitab, Yoshihiro Sakoda, Kyoko Tsukiyama-Kohara
Classical swine fever (CSF) is a fatal contagious disease affecting pigs caused by classical swine fever virus (CSFV). The disease can be transmitted by pigs and wild boars, and it is difficult to prevent and control. To obtain necessary information to establish the CSFV resistant animals in a future study, we designed lentiviral vector-delivered short hairpin RNAs (shRNAs) targeting the conserved
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Solubility of lamotrigine in age-specific biorelevant media that simulated the fasted- and fed-conditions of the gastric and intestinal environments in pediatrics and adults: implications for traditional, re-formulated, modified, and new oral formulations BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-08 Ramzi Shawahna, Hala Saba’aneh, Amal Daraghmeh, Yara Qassarwi, Valentina Franco, Xavier Declèves
Lamotrigine is an effective antiseizure medication that can be used in the management of focal and generalized epilepsies in pediatric patients. This study was conducted to quantify and compare the solubility of lamotrigine in age-specific biorelevant media that simulated the fasted and fed conditions of the gastric and intestinal environments in pediatrics and adults. Another aim was to predict how
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Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6 BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-08 David I. Gonzalez, Ruby A. Ynalvez
Biofuel research that aims to optimize growth conditions in microalgae is critically important. Chlamydomonas reinhardtii is a green microalga that offers advantages for biofuel production research. This study compares the effects of nitrogen-, sulfur-, and nitrogen and sulfur- deprivations on the C. reinhardtii starchless mutant cc5373-sta6. Specifically, it compares growth, lipid body accumulation
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Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks BMC Biotechnol. (IF 3.5) Pub Date : 2023-09-03 Paul-Joachim Niehoff, Waldemar Müller, Johannes Pastoors, Katharina Miebach, Philipp Ernst, Johannes Hemmerich, Stephan Noack, Nick Wierckx, Jochen Büchs
Currently, Aspergillus terreus is used for the industrial production of itaconic acid. Although, alternative feedstock use in fermentations is crucial for cost-efficient and sustainable itaconic acid production, their utilisation with A. terreus most often requires expensive pretreatment. Ustilaginacea are robust alternatives for itaconic acid production, evading the challenges, including the pretreatment
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Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-29 Siyuan Gao, Ding Ma, Yongtao Wang, Alei Zhang, Xin Wang, Kequan Chen
Dopamine is high-value compound of pharmaceutical interest, but its industrial scale production mostly focuses on chemical synthesis, possessing environment pollution. Bio-manufacturing has caused much attention for its environmental characteristic. Resting cells were employed to as biocatalysts with extraordinary advantages like offering stable surroundings, the inherent presence of expensive cofactors
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An effective device to enable consistent scratches for in vitro scratch assays BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-28 Sixun Chen, Ahmad Amirul bin Abdul Rahim, Pamela Mok, Dan Liu
The in-vitro scratch assay is a useful method in wound healing research to assess cell migration. In this assay, a scratch is created in a confluent cell layer by mechanically removing cells through manual scraping with a sharp-edged tool. This step is traditionally done with pipette tips and is unsuitable for high-throughput assays, as the created scratches are highly variable in width and position
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Highly multiplexed targeted sequencing strategy for infectious disease surveillance BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-23 Iván Hernández-Neuta, Anastasia Magoulopoulou, Flor Pineiro, Jan Gorm Lisby, Mats Gulberg, Mats Nilsson
Global efforts to characterize diseases of poverty are hampered by lack of affordable and comprehensive detection platforms, resulting in suboptimal allocation of health care resources and inefficient disease control. Next generation sequencing (NGS) can provide accurate data and high throughput. However, shotgun and metagenome-based NGS approaches are limited by low concentrations of microbial DNA
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Determination of serum CA724 levels using fluorescence immunochromatography BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-18 Chang Liu, Cuicui Chen, Fenglan Peng, Huankun Liang, Shuhai Zhong, Tiancai Liu, Laiqing Li, Alexander Boronin, Wenqi Dong
Carbohydrate antigen 724 (CA724) is a sensitive and specific indicator for multiple malignant tumors. The aim of this study was to establish a Eu-time resolved fluorescence immunochromatography (Eu-TRFICO) method for quantitative detection of CA724 in serum. Eu-TRFICO strips were optimized and assembled. The sensitivity, specificity and precision were evaluated using CA724 standard dilutions and matrix
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Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-16 Weiwei Liu, Xiaoguo Wang, Ruirong Liu, Yaya Liao, Zhiwei Peng, Haoyun Jiang, Qiqi Jing, Yuyun Xing
Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered. In this study, we aimed to optimize the transfection conditions for delivery of a large-size vector in PFFs using Lonza 4D-Nucleofector™ vessels and strips. We firstly delivered
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A nano-Liposomal formulation potentiates antioxidant, anti-inflammatory, and fibrinolytic activities of Allolobophora caliginosa coelomic fluid: formulation and characterization BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-03 Asmaa E. Farouk, Sohair R. Fahmy, Amel M. Soliman, Sherif Abdelaziz Ibrahim, Shimaa A. Sadek
Coelomic fluid, a pharmacologically active compound in earthworms, exhibits a range of biological activities, including antioxidant, anti-inflammatory, and anticancer. However, the biological activities exerted by the coelomic fluid can be restrained by its low bioavailability and stability. Liposomes are progressively utilized as an entrapment system for natural bioactive compounds with poor bioavailability
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Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms BMC Biotechnol. (IF 3.5) Pub Date : 2023-08-01 Marziyeh Jafari, Mohammad Hosein Daneshvar
Optimization of indirect shoot regeneration protocols is one of the key prerequisites for the development of Agrobacterium-mediated genetic transformation and/or genome editing in Passiflora caerulea. Comprehensive knowledge of indirect shoot regeneration and optimized protocol can be obtained by the application of a combination of machine learning (ML) and optimization algorithms. In the present investigation
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The impact of applying various de novo assembly and correction tools on the identification of genome characterization, drug resistance, and virulence factors of clinical isolates using ONT sequencing BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-31 Hussain A. Safar, Fatemah Alatar, Kother Nasser, Rehab Al-Ajmi, Wadha Alfouzan, Abu Salim Mustafa
Oxford Nanopore sequencing technology (ONT) is currently widely used due to its affordability, simplicity, and reliability. Despite the advantage ONT has over next-generation sequencing in detecting resistance genes in mobile genetic elements, its relatively high error rate (10–15%) is still a deterrent. Several bioinformatic tools are freely available for raw data processing and obtaining complete
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Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-28 Jennifer Goldmanns, Georg Andreas Röhling, Marie Kristine Lipa, Theresa Scholand, Alexander Deitert, Tobias May, Evangeline Priya Haas, Matthias Boy, Andrea Herold, Jochen Büchs
One critical parameter in microbial cultivations is the composition of the cultivation medium. Nowadays, the application of chemically defined media increases, due to a more defined and reproducible fermentation performance than in complex media. In order, to improve cost-effectiveness of fermentation processes using chemically defined media, the media should not contain nutrients in large excess.
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Development of a mammalian cell-based ZZ display system for IgG quantification BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-28 Lingzhi Bao, Aizhen Yang, Ziqing Liu, Jie Ma, Jiajie Pan, Yi Zhu, Ying Tang, Pu Dong, Guoping Zhao, Shaopeng Chen
Biological laboratories and companies involved in antibody development need convenient and versatile methods to detect highly active antibodies. To develop a mammalian cell-based ZZ display system for antibody quantification, the eukaryotic ZZ-displayed plasmid was constructed and transfected into CHO cells. After screening by flow cytometric sorting, the stable ZZ display cells were incubated with
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Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-20 Elsayed Elgazzar, H. A. Ayoub, Z. A. El-Wahab, Wageha A. Mostafa
Infectious diseases prompted by micro-organisms such as fungi, parasites, or microbes, have influenced many countries’ public health causing death. Scientists declared that metal oxide composites have various advantages in the medical field such as the antimicrobial feature has freshly been revealed as well as its role in suppressing mosquito population. In this work silver doped zinc oxide nanorods
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Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-14 Nan Li, Jie Lu, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang
L-lysine is widely used for feed and special diet products. The transformation of fermentation strains plays a decisive role in the development of these industries. Based on the mutation breeding theory and metabolic engineering methods, this study aimed to improve the regeneration rate of high-lethality protoplasts by combining multiple mutagenesis and homologous cell fusion techniques to efficiently
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Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-11 Nahideh Asadi, Hadi Sadeghzadeh, Azizeh Rahmani Del Bakhshayesh, Amir Nezami Asl, Mehdi Dadashpour, Negar Karimi Hajishoreh, Sharif Kaamyabi, Abolfazl Akbarzadeh
Gelatin methacrylate-based hydrogels (GelMA) were widely used in tissue engineering and regenerative medicine. However, to manipulate their various chemical and physical properties and create high-efficiency hydrogels, different materials have been used in their structure. Eggshell membrane (ESM) and propolis are two nature-derived materials that could be used to improve the various characteristics
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Lipid extraction and analysis of microalgae strain pectinodesmus PHM3 for biodiesel production BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-10 Dania Akram Kiyani, Shafia Maryam, Sundus Jabeen Amina, Abdullah Ahmad, Muhammad Waqas Alam Chattha, Hussnain Ahmed Janjua
The current study is focused on the lipid extract of microalgae; Pectinodesmus strain PHM3 and its general analysis in terms of chemical contents. Combinations of both chemical and mechanistic approaches were applied to obtain the maximum yield of lipids which was recorded to be 23% per gram through continuous agitation using Folch solution. The extraction methods used in this study included: Bligh
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Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-08 Sven Reislöhner, Geza Schermann, Max Kilian, Daniela Santamaría-Muñoz, Christian Zimmerli, Nikola Kellner, Jochen Baßler, Michael Brunner, Ed Hurt
The thermophilic fungus Chaetomium thermophilum has been used extensively for biochemical and high-resolution structural studies of protein complexes. However, subsequent functional analyses of these assemblies have been hindered owing to the lack of genetic tools compatible with this thermophile, which are typically suited to other mesophilic eukaryotic model organisms, in particular the yeast Saccharomyces
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A computational approach to optimising laccase-mediated polyethylene oxidation through carbohydrate-binding module fusion BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-06 Michael Gollan, Gary Black, Jose Munoz-Munoz
Plastic pollution is a major global concern to the health and wellbeing of all terrestrial and marine life. However, no sustainable method for waste management is currently viable. This study addresses the optimisation of microbial enzymatic polyethylene oxidation through rational engineering of laccases with carbohydrate-binding module (CBM) domains. An explorative bioinformatic approach was taken
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Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) BMC Biotechnol. (IF 3.5) Pub Date : 2023-07-04 Snigdha Samanta, Mritunjoy Barman, Himanshu Thakur, Swati Chakraborty, Gouranga Upadhyaya, Deepayan Roy, Amitava Banerjee, Arunava Samanta, Jayanta Tarafdar
The invasive and calamitous polyphagous pest Spodoptera frugiperda or commonly known as fall armyworm (FAW) poses serious menace to the global agricultural production. Owing to the revamped invasion of FAW in 2018 in India, present study was undertaken for precise assessment of its genetic identity and pesticide resistance to aid in pest-management strategies. To evaluate the diversity in FAW population
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The feasibility of sodium hydroxide pretreatment of rice straw for solid substrate preparation to enhance laccase production by solid state fermentation BMC Biotechnol. (IF 3.5) Pub Date : 2023-06-30 Lulu Wang, Ni An, Junting Gao, Huiting Xue, Guanhua Li
Currently, broad industrial application of laccases is commonly restricted by the high-cost related production. Solid state fermentation (SSF) using agricultural waste is an attractively economic strategy for laccase production, yet its efficiency is low. Pretreatment of cellulosic substrate might be a vital breakpoint to solve the problem in solid state fermentation (SSF). In this study, sodium hydroxide
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Expression and characterization of recombinant IL-1Ra in Aspergillus oryzae as a system BMC Biotechnol. (IF 3.5) Pub Date : 2023-06-20 Lena Mahmoudi Azar, Elif Karaman, Burcu Beyaz, Işılay Göktan, Alp Ertunga Eyüpoğlu, Seda Kizilel, Batu Erman, Ahmet Gül, Serdar Uysal
The interleukin-1 receptor antagonist (IL-1Ra) is a crucial molecule that counteracts the effects of interleukin-1 (IL-1) by binding to its receptor. A high concentration of IL-1Ra is required for complete inhibition of IL-1 activity. However, the currently available Escherichia coli-expressed IL-1Ra (E. coli IL-1Ra, Anakinra) has a limited half-life. This study aims to produce a cost-effective, functional
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Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter BMC Biotechnol. (IF 3.5) Pub Date : 2023-06-07 Matthias Knödler, Paul Winman Reunious, Johannes Felix Buyel
Large-scale transient expression of recombinant proteins in plants is increasingly used and requires the multi-liter cultivation of Agrobacterium tumefaciens transformed with an expression vector, which is often cloned in Escherichia coli first. Depending on the promoter, unintentional activity can occur in both bacteria, which could pose a safety risk to the environment and operators if the protein
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Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds BMC Biotechnol. (IF 3.5) Pub Date : 2023-06-06 Lin Jin, Jiali Shan, Yanhong Hao, Yingchun Wang, Liping Liu
To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed scaffolds. BMSCs were irradiated with different LIPUS parameters and microbubble concentrations, and the best acoustic excitation
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A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility BMC Biotechnol. (IF 3.5) Pub Date : 2023-05-01 Prem Kumar Govindappa, Mosammat Begom, Yash Gupta, John C. Elfar, Manmeet Rawat, Walaa Elfar
Dysmotility and postoperative ileus (POI) are frequent major clinical problems post-abdominal surgery. Erythropoietin (EPO) is a multifunctional tissue-protective cytokine that promotes recovery of the intestine in various injury models. While EPO receptors (EPOR) are present in vagal Schwann cells, the role of EPOR in POI recovery is unknown because of the lack of EPOR antagonists or Schwann-cell
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Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins BMC Biotechnol. (IF 3.5) Pub Date : 2023-04-05 Tess Lamer, John C. Vederas
Some peptides are targets for degradation when heterologously expressed as fusion proteins in E. coli, which can limit yields after isolation and purification. We recently reported that peptide degradation may be prevented by production of a “sandwiched” SUMO-peptide-intein (SPI) fusion protein, which protects the target peptide sequence from truncation and improves yield. This initial system required
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Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis, infesting hot peppers BMC Biotechnol. (IF 3.5) Pub Date : 2023-04-04 Falguni Khan, Minlee Kim, Yonggyun Kim
The western flower thrips Frankliniella occidentalis is an insect pest that damages various crops, including hot peppers. It is a vector of a plant pathogen, tomato spotted wilt virus. To control this pest, chemical insecticides have been used in the past, but the control efficacy is unsatisfactory owing to rapid resistance development by F. occidentalis. : This study reports a novel control technology