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Tripartite collaboration of blood‐derived endothelial cells, next generation RNA sequencing and bioengineered vessel‐chip may distinguish vasculopathy and thrombosis among sickle cell disease patients Bioeng. Transl. Med. (IF 6.091) Pub Date : 2021-01-06 Tanmay Mathur; Jonathan M. Flanagan; Abhishek Jain
Sickle cell disease (SCD) is the most prevalent inherited blood disorder in the world. But the clinical manifestations of the disease are highly variable. In particular, it is currently difficult to predict the adverse outcomes within patients with SCD, such as, vasculopathy, thrombosis, and stroke. Therefore, for most effective and timely interventions, a predictive analytic strategy is desirable
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BioTM Buzz (Volume 6, Issue 1) Bioeng. Transl. Med. (IF 6.091) Pub Date : 2021-01-08 Aaron C. Anselmo
1 NANOPARTICLE FOR TARGETING CARTILAGE IN OSTEOARTHRITIS Current treatments for osteoarthritis are limited in their ability to penetrate and persist within the cartilage matrix; furthermore, existing therapies primarily focus on managing pain and modulating joint function as opposed to therapeutic treatment. As such, there is a considerable need for the development of new therapeutics and delivery
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Mitochondrial transplantation therapy inhibit carbon tetrachloride‐induced liver injury through scavenging free radicals and protecting hepatocytes Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-12-17 Zizhen Zhao; Yixue Hou; Wei Zhou; Rajendiran Keerthiga; Ailing Fu
Carbon tetrachloride (CCl4)‐induced liver injury is predominantly caused by free radicals, in which mitochondrial function of hepatocytes is impaired, accompanying with the production of ROS and decreased ATP energy supply in animals intoxicated with CCl4. Here we explored a novel therapeutic approach, mitochondrial transplantation therapy, for treating the liver injury. The results showed that mitochondria
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Microscopy‐guided laser ablation for the creation of complex skin models with folliculoid appendages Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-30 Carla M. Abreu; Luca Gasperini; Manuela E. L. Lago; Rui L. Reis; Alexandra P. Marques
Engineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue's 3D microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle (HF) appendages have highlighted the importance of facilitating direct interaction between dermal papilla (DP) cells
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A review of biomimetic scaffolds for bone regeneration: Toward a cell‐free strategy Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-12-02 Sijing Jiang; Mohan Wang; Jiacai He
In clinical terms, bone grafting currently involves the application of autogenous, allogeneic, or xenogeneic bone grafts, as well as natural or artificially synthesized materials, such as polymers, bioceramics, and other composites. Many of these are associated with limitations. The ideal scaffold for bone tissue engineering should provide mechanical support while promoting osteogenesis, osteoconduction
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Intradermal delivery of receptor‐binding domain of SARS‐CoV‐2 spike protein with dissolvable microneedles to induce humoral and cellular responses in mice Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-25 Chaiyaporn Kuwentrai; Jinming Yu; Li Rong; Bao‐Zhong Zhang; Ye‐Fan Hu; Hua‐Rui Gong; Ying Dou; Jian Deng; Jian‐Dong Huang; Chenjie Xu
The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike protein contains an immunogenic receptor‐binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S‐RBD vaccine using a dissolvable microneedle skin patch can induce both significant B‐cell and significant T‐cell responses
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Targeted systemic dendrimer delivery of CSF‐1R inhibitor to tumor‐associated macrophages improves outcomes in orthotopic glioblastoma Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-12-01 Kevin Liaw; Rajsekhar Reddy; Anjali Sharma; Jiangyu Li; Michelle Chang; Rishi Sharma; Sebastian Salazar; Sujatha Kannan; Rangaramanujam M. Kannan
Glioblastoma is the most common and aggressive form of primary brain cancer, with median survival of 16–20 months and a 5‐year survival rates of <5%. Recent advances in immunotherapies have shown that addressing the tumor immune profile by targeting the colony‐stimulating factor 1 (CSF‐1) signaling pathway of tumor‐associated macrophages (TAMs) has the potential to improve glioblastoma therapy. However
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Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-30 Kunal S. Parikh; Revaz Omiadze; Aditya Josyula; Richard Shi; Nicole M. Anders; Ping He; Youseph Yazdi; Peter J. McDonnell; Laura M. Ensign; Justin Hanes
Sutures are applied almost universally at the site of trauma or surgery, making them an ideal platform to modulate the local, postoperative biological response, and improve surgical outcomes. To date, the only globally marketed drug‐eluting sutures are coated with triclosan for antibacterial application in general surgery. Loading drug directly into the suture rather than coating the surface offers
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A review of biomarkers in the context of type 1 diabetes: Biological sensing for enhanced glucose control Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-16 Kelilah L. Wolkowicz; Eleonora M. Aiello; Eva Vargas; Hazhir Teymourian; Farshad Tehrani; Joseph Wang; Jordan E. Pinsker; Francis J. Doyle; Mary‐Elizabeth Patti; Lori M. Laffel; Eyal Dassau
As wearable healthcare monitoring systems advance, there is immense potential for biological sensing to enhance the management of type 1 diabetes (T1D). The aim of this work is to describe the ongoing development of biomarker analytes in the context of T1D. Technological advances in transdermal biosensing offer remarkable opportunities to move from research laboratories to clinical point‐of‐care applications
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A Method to Improve Quantitative Radiotracing‐Based Analysis of the In Vivo Biodistribution of Drug Carriers Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-12-06 Nikša Roki; Melani Solomon; Lou Casta; Jessica Bowers; Robert C. Getts; Silvia Muro
Biodistribution studies are essential in drug carrier design and translation, and radiotracing provides a sensitive quantitation for this purpose. Yet, for biodegradable formulations, small amounts of free‐label signal may arise prior to‐ or immediately after injection in animal models, causing potentially confounding biodistribution results. In this study, we refined a method to overcome this obstacle
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Biofabricated 3D in vitro model of fibrosis‐induced abnormal hepatoblast/biliary progenitors’ expansion of the developing liver Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-12-06 Matthew Brovold; Dale Keller; Mahesh Devarasetty; Anthony Dominijanni; Rohan Shirwaiker; Shay Soker
Congenital disorders of the biliary tract are the primary reason for pediatric liver failure and ultimately for pediatric liver transplant needs. Not all causes of these disorders are well understood, but it is known that liver fibrosis occurs in many of those afflicted. The goal of this study is to develop a simple yet robust model that recapitulates physico‐mechanical and cellular aspects of fibrosis
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Analysis of aqueous humor total antioxidant capacity and its correlation with corneal endothelial health Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-12 Yu‐Ting Tsao; Wei‐Chi Wu; Kuan‐Jen Chen; Lung‐Kun Yeh; Yih‐Shiou Hwang; Yi‐Jen Hsueh; Hung‐Chi Chen; Chao‐Min Cheng
Corneal endothelial decompensation is a serious condition that frequently requires treatment via corneal transplantation which contributes to a global shortage in donor corneas. Therefore, the purpose of this study was to analyze the influence of aqueous humor total antioxidant capacity (TAC) on corneal endothelial health. There is an urgent need for discovering protective factors to combat corneal
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Doxorubicin‐loaded nanoparticle coated with endothelial cells‐derived exosomes for immunogenic chemotherapy of glioblastoma Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-26 Chao Zhang; Jian Song; Lei Lou; Xuejiao Qi; Lei Zhao; Bo Fan; Guozhu Sun; Zhongqiang Lv; Zhenzeng Fan; Baohua Jiao; Jiankai Yang
Treatments of glioblastoma (GBM) have not been very effective, largely due to the inefficiency of drugs in penetrating the blood brain barrier (BBB). In this study, we investigated the potential of exosome‐coated doxorubicin (DOX)‐loaded nanoparticles (ENPDOX) in BBB penetration, inducing immunogenic cell death (ICD) and promoting survival of GBM‐bearing mice. DOX‐loaded nanoparticles (NPDOX) were
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IDentif.AI: Rapidly optimizing combination therapy design against severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐Cov‐2) with digital drug development Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-10 Agata Blasiak; Jhin Jieh Lim; Shirley Gek Kheng Seah; Theodore Kee; Alexandria Remus; De Hoe Chye; Pui San Wong; Lissa Hooi; Anh T. L. Truong; Nguyen Le; Conrad E. Z. Chan; Rishi Desai; Xianting Ding; Brendon J. Hanson; Edward Kai‐Hua Chow; Dean Ho
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) led to multiple drug repurposing clinical trials that have yielded largely uncertain outcomes. To overcome this challenge, we used IDentif.AI, a platform that pairs experimental validation with artificial intelligence (AI) and digital drug development to rapidly pinpoint unpredictable drug interactions and optimize infectious
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Transcriptomic response of brain tissue to focused ultrasound‐mediated blood–brain barrier disruption depends strongly on anesthesia Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-10 Alexander S. Mathew; Catherine M. Gorick; E. Andrew Thim; William J. Garrison; Alexander L. Klibanov; G. Wilson Miller; Natasha D. Sheybani; Richard J. Price
Focused ultrasound (FUS) mediated blood–brain barrier disruption (BBBD) targets the delivery of systemically‐administered therapeutics to the central nervous system. Preclinical investigations of BBBD have been performed on different anesthetic backgrounds; however, the influence of the choice of anesthetic on the molecular response to BBBD is unknown, despite its potential to critically affect interpretation
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Engineered macrophage membrane‐enveloped nanomedicine for ameliorating myocardial infarction in a mouse model Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-10 Yugang Xue; Guangwei Zeng; Jin Cheng; Jianqiang Hu; Mingming Zhang; Yan Li
Myocardial infarction (MI) is the serious condition causing lots of death over the world. Myocytes apoptosis, inflammation, and fibrosis are three important factors implicated in pathogenesis of MI. Targeting these three factors has been shown to ameliorate MI and rescue cardiac function. Previous studies have demonstrated that microRNA (miR) 199a‐3p protect against MI. In this study, we prepare macrophage
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Screening of important metabolites and KRAS genotypes in colon cancer using secondary ion mass spectrometry Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-11-11 Kookrae Cho; Eun‐Sook Choi; Sung Young Lee; Jung‐Hee Kim; Dae Won Moon; Jong‐Wuk Son; Eunjoo Kim
Time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) is an imaging‐based analytical technique that can characterize the surfaces of biomaterials. We used TOF‐SIMS to identify important metabolites and oncogenic KRAS mutation expressed in human colorectal cancer (CRC). We obtained 540 TOF‐SIMS spectra from 180 tissue samples by scanning cryo‐sections and selected discriminatory molecules using
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Validation of the 1,4‐butanediol thermoplastic polyurethane as a novel material for 3D bioprinting applications Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-20 Carlos Chocarro‐Wrona; Juan de Vicente; Cristina Antich; Gema Jiménez; Daniel Martínez‐Moreno; Esmeralda Carrillo; Elvira Montañez; Patricia Gálvez‐Martín; Macarena Perán; Elena López‐Ruiz; Juan Antonio Marchal
Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires the development of functional personalized implants with cartilage‐like mechanical properties capable of sustaining high load‐bearing environments to integrate into the surrounding tissue of the cartilage defect. In this study, we evaluated the
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Effect of an alkyl spacer on the morphology and internalization of MUC1 aptamer‐naphthalimide amphiphiles for targeting and imaging triple negative breast cancer cells Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-21 Huihui Kuang; Zachary Schneiderman; Ahmed M. Shabana; Gabriella C. Russo; Jun Guo; Denis Wirtz; Efrosini Kokkoli
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Clinical translation of choline and geranic acid deep eutectic solvent Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-16 Justin Ko; Abhirup Mandal; Sunil Dhawan; Marina Shevachman; Samir Mitragotri; Nitin Joshi
Choline geranate deep eutectic solvent/ionic liquid (CAGE) has shown several desirable therapeutic properties including antimicrobial activity and ability to deliver drugs transdermally in research laboratories. Here, we describe the first report of clinical translation of CAGE from the lab into the clinic for the treatment of rosacea, a common chronic inflammatory skin disorder that affects the face
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Sonodynamic therapy reduces iron retention of hemorrhagic plaque Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-20 Bicheng Li; Jie Gong; Siqi Sheng; Minqiao Lu; Shuyuan Guo; Jianting Yao; Haiyu Zhang; Xuezhu Zhao; Zhengyu Cao; Xin Sun; Huan Wang; Yang Cao; Yongxing Jiang; Zhen Tian; Bin Liu; Hua Zhao; Zhiguo Zhang; Hong Jin; Ye Tian
Intraplaque hemorrhage (IPH) plays a major role in the aggressive progression of vulnerable plaque, leading to acute cardiovascular events. We previously demonstrated that sonodynamic therapy (SDT) inhibits atherosclerotic plaque progression. In this study, we investigated whether SDT could also be applied to treat more advanced hemorrhagic plaque and addressed the underlying mechanism. SDT decreased
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Magnetic microgels and nanogels: Physical mechanisms and biomedical applications Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-10-09 Baeckkyoung Sung; Min‐Ho Kim; Leon Abelmann
Soft micro‐ and nanostructures have been extensively developed for biomedical applications. The main focus has been on multifunctional composite materials that combine the advantages of hydrogels and colloidal particles. Magnetic microgels and nanogels can be realized by hybridizing stimuli‐sensitive gels and magnetic nanoparticles. They are of particular interest since they can be controlled in a
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Ultrasound‐microbubble cavitation facilitates adeno‐associated virus mediated cochlear gene transfection across the round‐window membrane Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-09-18 Zhen Zhang; Zhengnong Chen; Liqiang Fan; Thomas Landry; Jeremy Brown; Zhiping Yu; Shankai Yin; Jian Wang
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Bioengineering & Translational Medicine: Year 2020 in review Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-26 Samir Mitragotri
2020 marks an important year in the evolution of BioTM. We received our first impact factor of 6.091 this year. This is a massive accomplishment for all stakeholders of the journal. The journal was formally launched in 2015 and we published our first issue in 2016. Within a short period of 4 years, we were able to reach this significant milestone. Huge thanks to all BioTM authors who trusted the journal
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Introduction to Editorial Board Member: Professor W. Mark Saltzman Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-07-29 Steven M. Jay
In this issue of Bioengineering and Translational Medicine, we are pleased to introduce our Editorial Board Member, Professor W. Mark Saltzman. Professor Saltzman is the Goizueta Foundation Professor of Biomedical Engineering, Chemical and Environmental Engineering, and Physiology at Yale University. Professor Saltzman received his BS in chemical engineering from Iowa State University before earning
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BioTM Buzz (Volume 5, Issue 3): The Future is Bright Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-29 Samir Mitragotri; Aaron C. Anselmo
No one can predict the future, but that is not going to stop us from taking a shot at it. At BioTM, we do wonder what the future holds for our key scientific areas, including drug delivery, tissue engineering, cell therapy, and biosensors, among many others. Ultimately, the future will become what the future scientists aspire to do. With this realization, we figured that our best shot at predicting
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Current diagnostic tools for coronaviruses-From laboratory diagnosis to POC diagnosis for COVID-19. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-03 Yung-Chih Wang,Yi-Tzu Lee,Ting Yang,Jun-Ren Sun,Ching-Fen Shen,Chao-Min Cheng
The Coronavirus‐2019 (COVID‐19) pandemic has put tremendous strain on healthcare systems worldwide. It is challenging for clinicians to differentiate COVID‐19 from other acute respiratory tract infections via clinical symptoms because those who are infected display a wide range of symptoms. An effective, point‐of‐care (POC) diagnostic tool could mitigate healthcare system strain, protect healthcare
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Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-20 Dhananjay V. Deshmukh; Nils Pasquero; Gajraj Rathore; Joel Zvick; Ori Bar‐Nur; Jurg Dual; Mark W. Tibbitt
Hydrogel‐based three‐dimensional (3D) cellular models are attractive for bioengineering and pharmaceutical development as they can more closely resemble the cellular function of native tissue outside of the body. In general, these models are composed of tissue specific cells embedded within a support material, such as a hydrogel. As hydrogel properties directly affect cell function, hydrogel composition
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The effect of size, charge, and peptide ligand length on kidney targeting by small, organic nanoparticles Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-07-21 Yi Huang; Kairui Jiang; Xuting Zhang; Eun Ji Chung
Chronic kidney disease (CKD) affects 15% of the US adult population. However, most clinically available drugs for CKD show low bioavailability to the kidneys and non‐specific uptake by other organs which results in adverse side effects. Hence, a targeted, drug delivery strategy to enhance kidney drug delivery is highly desired. Recently, our group developed small, organic nanoparticles called peptide
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Nanoparticle‐microglial interaction in the ischemic brain is modulated by injury duration and treatment Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-01 Andrea Joseph; Rick Liao; Mengying Zhang; Hawley Helmbrecht; Michael McKenna; Jeremy R. Filteau; Elizabeth Nance
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In situ forming microporous gelatin methacryloyl hydrogel scaffolds from thermostable microgels for tissue engineering Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-10 Nicole Zoratto; Donatella Di Lisa; Joseph de Rutte; Md Nurus Sakib; Angelo Roncalli Alves e Silva; Ali Tamayol; Dino Di Carlo; Ali Khademhosseini; Amir Sheikhi
Converting biopolymers to extracellular matrix (ECM)‐mimetic hydrogel‐based scaffolds has provided invaluable opportunities to design in vitro models of tissues/diseases and develop regenerative therapies for damaged tissues. Among biopolymers, gelatin and its crosslinkable derivatives, such as gelatin methacryloyl (GelMA), have gained significant importance for biomedical applications due to their
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Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial NADPH pool under mitochondrial oxidative stress Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-24 Sun Jin Moon; Wentao Dong; Gregory N. Stephanopoulos; Hadley D. Sikes
Mitochondrial NADPH protects cells against mitochondrial oxidative stress by serving as an electron donor to antioxidant defense systems. However, due to technical challenges, it still remains unknown as to the pool size of mitochondrial NADPH, its dynamics, and NADPH/NADP+ ratio. Here, we have systemically modulated production rates of H2O2 in mitochondria and assessed mitochondrial NADPH metabolism
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The secretome of stressed peripheral blood mononuclear cells increases tissue survival in a rodent epigastric flap model Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-30 Stefan Hacker; Rainer Mittermayr; Denise Traxler; Claudia Keibl; Annika Resch; Stefan Salminger; Harald Leiss; Philipp Hacker; Christian Gabriel; Bahar Golabi; Reinhard Pauzenberger; Paul Slezak; Maria Laggner; Michael Mildner; Wolfgang Michlits; Hendrik J. Ankersmit
Reconstructive surgery transfers viable tissue to cover defects and to restore aesthetic and functional properties. Failure rates after free flap surgery range from 3 to 7%. Co‐morbidities such as diabetes mellitus or peripheral vascular disease increase the risk of flap failure up to 4.5‐fold. Experimental therapeutic concepts commonly use a monocausal approach by applying single growth factors. The
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Gemcitabine and doxorubicin in immunostimulatory monophosphoryl lipid A liposomes for treating breast cancer Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-09-08 Debra Wu; Zongmin Zhao; Jayoung Kim; Amaya Razmi; Lily Li‐Wen Wang; Neha Kapate; Yongsheng Gao; Kevin Peng; Anvay Ukidve; Samir Mitragotri
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Cartilage‐targeting ultrasmall lipid‐polymer hybrid nanoparticles for the prevention of cartilage degradation Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-09-01 Xiangzhao Ai; Yaou Duan; Qiangzhe Zhang; Derrick Sun; Ronnie H. Fang; Ru Liu‐Bryan; Weiwei Gao; Liangfang Zhang
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Current status of targeted microbubbles in diagnostic molecular imaging of pancreatic cancer Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-22 Natacha Jugniot; Rakesh Bam; Emmanuelle J. Meuillet; Evan C. Unger; Ramasamy Paulmurugan
Pancreatic ductal adenocarcinoma (PDAC) is often associated with a poor prognosis due to silent onset, resistance to therapies, and rapid spreading. Most patients are ineligible for curable surgery as they present with advanced disease at the time of diagnosis. Present diagnostic methods relying on anatomical changes have various limitations including difficulty to discriminate between benign and malignant
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Resection and survival data from a clinical trial of glioblastoma multiforme‐specific IRDye800‐BBN fluorescence‐guided surgery Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-23 Kunshan He; Chongwei Chi; Deling Li; Jingjing Zhang; Gang Niu; Fangqiao Lv; Junmei Wang; Wenqiang Che; Liwei Zhang; Nan Ji; Zhaohui Zhu; Jie Tian; Xiaoyuan Chen
Supra‐maximum surgical tumor resection without neurological damage is highly valuable for treatment and prognosis of patients with glioblastoma multiforme (GBM). We developed a GBM‐specific fluorescence probe using IRDye800CW (peak absorption/emission, 778/795 nm) and bombesin (BBN), which (IRDye800‐BBN) targets the gastrin‐releasing peptide receptor, and evaluated the image‐guided resection efficiency
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A novel bilayered expanded polytetrafluoroethylene glaucoma implant creates a permeable thin capsule independent of aqueous humor exposure Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-08-07 Amanda Kiely Bicket; Julia Szeto; Peter Roeber; Jeff Towler; Mitch Troutman; E. Randy Craven; Anup Khatana; Ike Ahmed; Harry Quigley; Pradeep Ramulu; Ian F. Pitha
The purpose of these studies was to evaluate clinical, functional, and histopathological features of glaucoma drainage implants (GDIs) fabricated from novel, custom‐tailored expanded polytetrafluoroethylene (ePTFE). Implants of matching footprints were fabricated from silicone (Control) and novel, bilayered ePTFE. ePTFE implants included: (a) one that inflated with aqueous humor (AH) (High), (b) one
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Point‐of‐care semen analysis of patients with infertility via smartphone and colorimetric paper‐based diagnostic device Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-07-29 Yu‐Ting Tsao; Chung‐Yao Yang; Yun‐Chiao Wen; Ting‐Chang Chang; Koji Matsuura; Yu Chen; Chao‐Min Cheng
Male infertility affects millions of males worldwide and is rising in prevalence due to social and environmental conditions. However, men often feel too embarrassed to receive a semen analysis in the hospital due to social stigmas. To overcome this problem, we developed a 3‐(4,5‐Dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide test strip to distinguish semen samples with low total motile sperm
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Personalized‐induced neural stem cell therapy: Generation, transplant, and safety in a large animal model Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-07-01 Hunter N. Bomba; Kevin T. Sheets; Alain Valdivia; Simon Khagi; Laura Ruterbories; Christopher L. Mariani; Luke B. Borst; Debra A. Tokarz; Shawn D. Hingtgen
In this study, we take an important step toward clinical translation by generating the first canine‐induced neural stem cells (iNSCs). We explore key aspects of scale‐up, persistence, and safety of personalized iNSC therapy in autologous canine surgery models. iNSCs are a promising new approach to treat aggressive cancers of the brain, including the deadly glioblastoma. Created by direct transdifferentiation
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Therapeutic potential of extracellular vesicle‐associated long noncoding RNA Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-07-09 Louis J. Born; John W. Harmon; Steven M. Jay
Both extracellular vesicles (EVs) and long noncoding RNAs (lncRNAs) have been increasingly investigated as biomarkers, pathophysiological mediators, and potential therapeutics. While these two entities have often been studied separately, there are increasing reports of EV‐associated lncRNA activity in processes such as oncogenesis as well as tissue repair and regeneration. Given the powerful nature
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The wasp venom antimicrobial peptide polybia‐CP and its synthetic derivatives display antiplasmodial and anticancer properties Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-05-16 Marcelo D. T. Torres; Adriana F. Silva; Gislaine P. Andrade; Cibele N. Pedron; Giselle Cerchiaro; Anderson O. Ribeiro; Vani X. Oliveira; Cesar de la Fuente‐Nunez
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Hyaluronic acid–doxorubicin nanoparticles for targeted treatment of colorectal cancer Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-05-12 Daniel C. Pan; Vinu Krishnan; Alyssa K. Salinas; Jayoung Kim; Tao Sun; Sagi Ravid; Kevin Peng; Debra Wu; Md Nurunnabi; Jeffery A. Nelson; Zachary Niziolek; Junling Guo; Samir Mitragotri
Colorectal cancer, common in both men and women, occurs when tumors form in the linings of the colon. Common treatments of colorectal cancer include surgery, chemotherapy, and radiation therapy; however, many colorectal cancer treatments often damage healthy tissues and cells, inducing severe side effects. Conventional chemotherapeutic agents such as doxorubicin (Dox) can be potentially used for the
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PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-05-06 Xiaona Wen; Yu‐Chuan Ou; Holly F. Zarick; Xin Zhang; Anthony B. Hmelo; Quinton J. Victor; Eden P. Paul; Joseph M. Slocik; Rajesh R. Naik; Leon M. Bellan; Eugene C. Lin; Rizia Bardhan
Precise monitoring of specific biomarkers in biological fluids with accurate biodiagnostic sensors is critical for early diagnosis of diseases and subsequent treatment planning. In this work, we demonstrated an innovative biodiagnostic sensor, portable reusable accurate diagnostics with nanostar antennas (PRADA), for multiplexed biomarker detection in small volumes (~50 μl) enabled in a microfluidic
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Employing drug delivery strategies to create safe and effective pharmaceuticals for COVID-19. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-29 Kevin J McHugh
The outbreak of the novel SARS‐CoV‐2 pathogen and corresponding coronavirus disease 2019 (COVID‐19) have had an enormous impact on both global health and the daily lives of billions of people worldwide. With a proven vaccine at least a year from being fully tested for safety and efficacy, there may be an opportunity to rapidly repurpose existing drugs in order to prevent SARS‐CoV‐2 infections and improve
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BioTM buzz (volume 5, issue 2). Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-28 Aaron C Anselmo
1 DENDRIMER SIZE AND BRAIN TARGETING Nanotechnologies have demonstrated considerable success in targeting specific tissues and breaching biological barriers for the treatment of various cancers. As such, improving nanoparticle performance through optimization of their physicochemical properties represents one of the most widely investigated topics in preclinical nanotechnology research. In this issue
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Introduction to Editorial Board Member: Professor David J. Mooney Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-27 Ovijit Chaudhuri
In this issue of Bioengineering and Translational Medicine , we are pleased to introduce our Editorial Board Member, Professor David J. Mooney (Figure 1). Professor Mooney is the Robert P. Pinkas Family Professor of Bioengineering at the John A. Paulson School of Engineering and Applied Sciences at Harvard University. He is a founding member of the Wyss Institute for Biologically Inspired Engineering
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Nanoparticles containing constrained phospholipids deliver mRNA to liver immune cells in vivo without targeting ligands Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-26 Zubao Gan; Melissa P. Lokugamage; Marine Z. C. Hatit; David Loughrey; Kalina Paunovska; Manaka Sato; Ana Cristian; James E. Dahlman
Once inside the cytoplasm of a cell, mRNA can be used to treat disease by upregulating the expression of any gene. Lipid nanoparticles (LNPs) can deliver mRNA to hepatocytes in humans, yet systemic non‐hepatocyte delivery at clinical doses remains difficult. We noted that LNPs have historically been formulated with phospholipids containing unconstrained alkyl tails. Based on evidence that constrained
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Dendrimer size effects on the selective brain tumor targeting in orthotopic tumor models upon systemic administration. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-08 Kevin Liaw,Fan Zhang,Antonella Mangraviti,Sujatha Kannan,Betty Tyler,Rangaramanujam M Kannan
Malignant gliomas are the most common and aggressive form of primary brain tumors, with a median survival of 15–20 months for patients receiving maximal interventions. Advances in nanomedicine have provided tumor‐specific delivery of chemotherapeutics to potentially overcome their off‐target toxicities. Recent advances in dendrimer‐based nanomedicines have established that hydroxyl‐terminated poly(amidoamine)
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Inhalable bacteriophage powders: Glass transition temperature and bioactivity stabilization. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-04-07 Rachel Yoon Kyung Chang,Philip Chi Lip Kwok,Dipesh Khanal,Sandra Morales,Elizabeth Kutter,Jian Li,Hak-Kim Chan
Recent heightened interest in inhaled bacteriophage (phage) therapy for combating antibacterial resistance in pulmonary infections has led to the development of phage powder formulations. Although phages have been successfully bioengineered into inhalable powders with preserved bioactivity, the stabilization mechanism is yet unknown. This paper reports the first study investigating the stabilization
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Hydrogels in the clinic. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-03-26 Abhirup Mandal,John R Clegg,Aaron C Anselmo,Samir Mitragotri
Injectable hydrogels are one of the most widely investigated and versatile technologies for drug delivery and tissue engineering applications. Hydrogels’ versatility arises from their tunable structure, which has been enabled by considerable advances in fields such as materials engineering, polymer science, and chemistry. Advances in these fields continue to lead to invention of new polymers, new approaches
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A self-adhesive microneedle patch with drug loading capability through swelling effect. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-02-21 Sharon W T Chew,Ankur H Shah,Mengjia Zheng,Hao Chang,Christian Wiraja,Terry W J Steele,Chenjie Xu
Microneedles (MNs) offer a rapid method of transdermal drug delivery through penetration of the stratum corneum. However, commercial translation has been limited by fabrication techniques unique to each drug. Herein, a broadly applicable platform is explored by drug‐loading via swelling effect of a hydrogel MN patch. A range of small molecule hydrophilic, hydrophobic, and biomacromolecule therapeutics
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CRISPR/Cas9-mediated mutagenesis to validate the synergy between PARP1 inhibition and chemotherapy in BRCA1-mutated breast cancer cells. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2020-01-02 Rachel L Mintz,Yeh-Hsing Lao,Chun-Wei Chi,Siyu He,Mingqiang Li,Chai Hoon Quek,Dan Shao,Boyuan Chen,Jing Han,Sihong Wang,Kam W Leong
For patients carrying BRCA1 mutations, at least one-third develop triple negative breast cancer (TNBC). Not only is TNBC difficult to treat due to the lack of molecular target receptors, but BRCA1 mutations (BRCA1m) also result in chemotherapeutic resistance, making disease recurrence more likely. Although BRCA1m are highly heterogeneous and therefore difficult to target, BRCA1 gene's synthetic lethal
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Ex vivo isolated human vessel perfusion system for the design and assessment of nanomedicines targeted to the endothelium. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-18 Taras Lysyy,Laura G Bracaglia,Lingfeng Qin,Claire Albert,Jordan S Pober,George Tellides,W Mark Saltzman,Gregory T Tietjen
Endothelial cells play a central role in the process of inflammation. Their biologic relevance, as well as their accessibility to IV injected therapeutics, make them a strong candidate for treatment with molecularly‐targeted nanomedicines. Typically, the properties of targeted nanomedicines are first optimized in vitro in cell culture and then in vivo in rodent models. While cultured cells are readily
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Size, shape, and flexibility influence nanoparticle transport across brain endothelium under flow. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-15 Maksymilian Nowak,Tyler D Brown,Adam Graham,Matthew E Helgeson,Samir Mitragotri
Nanoparticle‐based therapeutic formulations are being increasingly explored for the treatment of various ailments. Despite numerous advances, the success of nanoparticle‐based technologies in treating brain diseases has been limited. Translational hurdles of nanoparticle therapies are attributed primarily to their limited ability to cross the blood–brain barrier (BBB), which is one of the body's most
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Sprayable and biodegradable, intrinsically adhesive wound dressing with antimicrobial properties. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-13 John L Daristotle,Lung W Lau,Metecan Erdi,Joseph Hunter,Albert Djoum,Priya Srinivasan,Xiaofang Wu,Mousumi Basu,Omar B Ayyub,Anthony D Sandler,Peter Kofinas
Conventional wound dressings are difficult to apply to large total body surface area (TBSA) wounds, as they typically are prefabricated, require a layer of adhesive coating for fixation, and need frequent replacement for entrapped exudate. Large TBSA wounds as well as orthopedic trauma and low-resource surgery also have a high risk of infection. In this report, a sprayable and intrinsically adhesive
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2D or 3D? How cell motility measurements are conserved across dimensions in vitro and translate in vivo. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-09 Sualyneth Galarza,Hyuna Kim,Naciye Atay,Shelly R Peyton,Jennifer M Munson
Cell motility is a critical aspect of several processes, such as wound healing and immunity; however, it is dysregulated in cancer. Current limitations of imaging tools make it difficult to study cell migration in vivo. To overcome this, and to identify drivers from the microenvironment that regulate cell migration, bioengineers have developed 2D (two-dimensional) and 3D (three-dimensional) tissue
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Targeting functionalized nanoparticles to activated endothelial cells under high wall shear stress. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-06 Hila Zukerman,Maria Khoury,Yosi Shammay,Josué Sznitman,Noah Lotan,Netanel Korin
Local inflammation of the endothelium is associated with a plethora of cardiovascular diseases. Vascular‐targeted carriers (VTCs) have been advocated to provide focal effective therapeutics to these disease sites. Here, we examine the design of functionalized nanoparticles (NPs) as VTCs that can specifically localize at an inflamed vessel wall under pathological levels of high shear stress, associated
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Remodeling adipose tissue inflammasome for type 2 diabetes mellitus treatment: Current perspective and translational strategies. Bioeng. Transl. Med. (IF 6.091) Pub Date : 2019-12-04 Amrita Banerjee,Jagdish Singh
Obesity‐associated type 2 diabetes mellitus (T2DM) is characterized by low‐grade chronic systemic inflammation that arises primarily from the white adipose tissue. The interplay between various adipose tissue‐derived chemokines drives insulin resistance in T2DM and has therefore become a subje