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Human induced pluripotent stem cells‐derived liver organoids grown on a Biomimesys® hyaluronic acid‐based hydroscaffold as a new model for studying human lipoprotein metabolism Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-16 Meryl Roudaut, Amandine Caillaud, Zied Souguir, Lise Bray, Aurore Girardeau, Antoine Rimbert, Mikaël Croyal, Gilles Lambert, Murielle Patitucci, Gaspard Delpouve, Élodie Vandenhaute, Cédric Le May, Nathalie Maubon, Bertrand Cariou, Karim Si‐Tayeb
The liver plays a key role in the metabolism of lipoproteins, controlling both production and catabolism. To accelerate the development of new lipid‐lowering therapies in humans, it is essential to have a relevant in vitro study model available. The current hepatocyte‐like cells (HLCs) models derived from hiPSC can be used to model many genetically driven diseases but require further improvement to
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Tolerability of a piezoelectric microneedle electroporator in human subjects Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-15 Chao‐Yi Lu, Pankaj Rohilla, Eric I. Felner, Gaurav Byagathvalli, Erkan Azizoglu, M. Saad Bhamla, Mark R. Prausnitz
Electroporation, or the use of electric pulses to facilitate the intracellular delivery of DNA, RNA, and other molecules, is a well‐established technique, that has been demonstrated to significantly augment the immunogenicity of DNA/mRNA vaccines and therapeutics. However, the clinical translation of traditional electroporators has been limited due to high costs, large size, complex user operation
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Evaluation of a novel vaginal cells self‐sampling device for human papillomavirus testing in cervical cancer screening: A clinical trial assessing reliability and acceptability Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-13 Chung‐Yao Yang, Ting‐Chang Chang, Hung‐Hsueh Chou, Angel Chao, Shih‐Tien Hsu, Yu‐Hsiang Shih, Huei‐Jean Huang, Cheng‐Tao Lin, Min‐Yu Chen, Lou Sun, Kuan‐Gen Huang, Kai‐Yun Wu, Wu‐Chiao Hsieh, Yi‐Ting Huang, Liang‐Hsuan Chen, Chien‐Hsing Lu, Hao Lin, Chao‐Min Cheng
Cervical cancer is a significant public health concern, particularly in low‐ and middle‐income countries where resources for prevention and treatment are limited. Routine screening, such as the Papanicolaou test (Pap smears) and human papillomavirus (HPV) testing, plays a crucial role in the early detection and prevention of cervical cancer. However, the participation rate in cervical cancer screening
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Synchronously in vivo real‐time monitoring bacterial load and temperature with evaluating immune response to decipher bacterial infection Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-13 Huaixuan Sheng, Huizhu Li, Shunyao Li, Chengxuan Yu, Yueming Wang, Haichen Hu, Lu Fang, Fuchun Chen, Yanyan Lu, Xiaogang Xu, Xing Yang, Shiyi Chen, Yuefeng Hao, Yunxia Li, Sijia Feng, Jun Chen
Determining the precise course of bacterial infection requires abundant in vivo real‐time data. Synchronous monitoring of the bacterial load, temperature, and immune response can satisfy the shortage of real‐time in vivo data. Here, we conducted a study in the joint‐infected mouse model to synchronously monitor the bacterial load, temperature, and immune response using the second near‐infrared (NIR‐II)
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Siglec15/TGF‐β bispecific antibody mediates synergistic anti‐tumor response against 4T1 triple negative breast cancer in mice Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-12 Limei Shen, Alison M. Schaefer, Karthik Tiruthani, Whitney Wolf, Samuel K. Lai
An ideal tumor‐specific immunomodulatory therapy should both preferentially target the tumor, while simultaneously reduce the immunosuppressive environment within the tumor. This guiding principle led us to explore engineering Siglec‐15 (S15) targeted bispecific antibody (bsAb) to enhance therapy against triple negative breast cancer (TNBC). S15 appears to be exclusively expressed on macrophages and
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Correction to “Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins” Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-03-11
Radhakrishnan H, Newmyer SL, Ssemadaali MA, Javitz HS, Bhatnagar P. Primary T-cell-based delivery platform for in vivo synthesis of engineered proteins. Bioeng Transl Med. 2024; 9(1):e10605. doi:10.1002/btm2.10605 Unlike the remainder of the manuscript where the data have been developed using pan CD3 T cells, Figure 4d–f used CD4 T cells. We apologize for this error. Corrections have been disclosed
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Supramolecular dye nanoassemblies for advanced diagnostics and therapies Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-02-13 Pouria Ramezani, Stefaan C. De Smedt, Félix Sauvage
Dyes have conventionally been used in medicine for staining cells, tissues, and organelles. Since these compounds are also known as photosensitizers (PSs) which exhibit photoresponsivity upon photon illumination, there is a high desire towards formulating these molecules into nanoparticles (NPs) to achieve improved delivery efficiency and enhanced stability for novel imaging and therapeutic applications
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Engineering a “muco-trapping” ACE2-immunoglobulin hybrid with picomolar affinity as an inhaled, pan-variant immunotherapy for COVID-19 Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-02-07 Karthik Tiruthani, Carlos Cruz-Teran, Jasper F. W. Chan, Alice Ma, Morgan McSweeney, Whitney Wolf, Shoufeng Yuan, Vincent K. M. Poon, Chris C. S. Chan, Lakshmi Botta, Brian Farrer, Ian Stewart, Alison Schaefer, Jasmine Edelstein, Priya Kumar, Harendra Arora, Jeff T. Hutchins, Anthony J. Hickey, Kwok-Yung Yuen, Samuel K. Lai
Soluble angiotensin-converting enzyme 2 (ACE2) can act as a decoy molecule that neutralizes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by blocking spike (S) proteins on virions from binding ACE2 on host cells. Based on structural insights of ACE2 and S proteins, we designed a “muco-trapping” ACE2-Fc conjugate, termed ACE2-(G4S)6-Fc, comprised of the extracellular segment of ACE2 (lacking
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Sex-dependent improvement in traumatic brain injury outcomes after liposomal delivery of dexamethasone in mice Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-02-04 Gherardo Baudo, Hannah Flinn, Morgan Holcomb, Anjana Tiwari, Sirena Soriano, Francesca Taraballi, Biana Godin, Assaf Zinger, Sonia Villapol
Traumatic brain injury (TBI) can have long-lasting physical, emotional, and cognitive consequences due to the neurodegeneration caused by its robust inflammatory response. Despite advances in rehabilitation care, effective neuroprotective treatments for TBI patients are lacking. Furthermore, current drug delivery methods for TBI treatment are inefficient in targeting inflamed brain areas. To address
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Tricaprylin-based drug crystalline suspension for intramuscular long-acting delivery of entecavir with alleviated local inflammation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-29 Min Young Jeong, Myoung Jin Ho, Joon Soo Park, Hoetaek Jeong, Jin Hee Kim, Yong Jin Jang, Doe Myung Shin, In Gyu Yang, Hye Rim Kim, Woo Heon Song, Sangkil Lee, Seh Hyon Song, Yong Seok Choi, Young Taek Han, Myung Joo Kang
In order to ensure prolonged pharmacokinetic profile along with local tolerability at the injection site, tricaprylin-based drug crystalline suspension (TS) was designed and its local distribution, pharmacokinetics, and inflammatory response, were evaluated with conventional aqueous suspension (AS). As model drug particles, entecavir 3-palmitate (EV-P), an ester lipidic prodrug for entecavir (EV),
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Engineered in vivo and in vitro tumor model recapitulates vasculogenic mimicry signatures in melanoma Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-27 Qizhi Shuai, Xinrui Xu, Yuxiang Liang, Zulala Halbiyat, Xin Lu, Zixuan Hu, Zhiwei Peng, Jie An, Zhiwei Feng, Tingjuan Huang, Hong Zhao, Zhizhen Liu, Jun Xu, Jun Xie
Vasculogenic mimicry (VM) describes a process by which tumor cells formed a novel microcirculation pattern in an endothelial cell-free manner. Clinically, VM is associated with aggressive phenotype and poor patient survival. However, the current models for investigating VM include 2D monolayer cultures, Matrigel-based cultures, and animal models, each of which has limitations. Matrigel-based models
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AI-organoid integrated systems for biomedical studies and applications Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-20 Sudhiksha Maramraju, Andrew Kowalczewski, Anirudh Kaza, Xiyuan Liu, Jathin Pranav Singaraju, Mark V. Albert, Zhen Ma, Huaxiao Yang
In this review, we explore the growing role of artificial intelligence (AI) in advancing the biomedical applications of human pluripotent stem cell (hPSC)-derived organoids. Stem cell-derived organoids, these miniature organ replicas, have become essential tools for disease modeling, drug discovery, and regenerative medicine. However, analyzing the vast and intricate datasets generated from these organoids
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Highly efficient CRISPR/Cas9-mediated exon skipping for recessive dystrophic epidermolysis bullosa Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-17 Alex du Rand, John Hunt, Christopher Samson, Evert Loef, Chloe Malhi, Sarah Meidinger, Chun-Jen Jennifer Chen, Ashley Nutsford, John Taylor, Rod Dunbar, Diana Purvis, Vaughan Feisst, Hilary Sheppard
Gene therapy based on the CRISPR/Cas9 system has emerged as a promising strategy for treating the monogenic fragile skin disorder recessive dystrophic epidermolysis bullosa (RDEB). With this approach problematic wounds could be grafted with gene edited, patient-specific skin equivalents. Precise gene editing using homology-directed repair (HDR) is the ultimate goal, however low efficiencies have hindered
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In situ light-activated materials for skin wound healing and repair: A narrative review Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-16 Jordan R. Yaron, Mallikarjun Gosangi, Shubham Pallod, Kaushal Rege
Dermal wounds are a major global health burden made worse by common comorbidities such as diabetes and infection. Appropriate wound closure relies on a highly coordinated series of cellular events, ultimately bridging tissue gaps and regenerating normal physiological structures. Wound dressings are an important component of wound care management, providing a barrier against external insults while preserving
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Micro Trojan horses: Engineering extracellular vesicles crossing biological barriers for drug delivery Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-11 Bin Zeng, Ying Li, Jiang Xia, Yin Xiao, Nawaz Khan, Bin Jiang, Yujie Liang, Li Duan
The biological barriers of the body, such as the blood–brain, placental, intestinal, skin, and air-blood, protect against invading viruses and bacteria while providing necessary physical support. However, these barriers also hinder the delivery of drugs to target tissues, reducing their therapeutic efficacy. Extracellular vesicles (EVs), nanostructures with a diameter ranging from 30 nm to 10 μm secreted
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Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-03 Ling Ren, Yuzhuo Zhao, Tiantian Wang, Yan Tong, Ping Zhao, Fang Nie, Yukun Luo, Lianhua Zhu
Microcirculatory perfusion disorder and inflammatory response are critical links in acute kidney injury (AKI). We aim to construct anti-vascular cell adhesion molecule-1(VCAM-1) targeted microbubbles (TM) to monitor renal microcirculatory perfusion and inflammatory response.
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Feasibility of engineered Bacillus subtilis for use as a microbiome-based topical drug delivery platform Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-02 Veronica A. Montgomery, Amy J. Wood-Yang, Mark P. Styczynski, Mark R. Prausnitz
Non-adherence to medication is a major challenge in healthcare that results in worsened treatment outcomes for patients. Reducing the frequency of required administrations could improve adherence but is challenging for topical drug delivery due to the generally short residence time of topical formulations on the skin. In this study, we sought to determine the feasibility of developing a microbiome-based
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An AI-assisted integrated, scalable, single-cell phenomic-transcriptomic platform to elucidate intratumor heterogeneity against immune response Bioeng. Transl. Med. (IF 7.4) Pub Date : 2024-01-02 Christopher P. Tostado, Lucas Xian Da Ong, Joel Jia Wei Heng, Carlo Miccolis, Shumei Chia, Justine Jia Wen Seow, Yi-Chin Toh, Ramanuj DasGupta
We present a novel framework combining single-cell phenotypic data with single-cell transcriptomic analysis to identify factors underpinning heterogeneity in antitumor immune response. We developed a pairwise, tumor-immune discretized interaction assay between natural killer (NK-92MI) cells and patient-derived head and neck squamous cell carcinoma (HNSCC) cell lines on a microfluidic cell-trapping
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Trafficking through the blood–brain barrier is directed by core and outer surface components of layer-by-layer nanoparticles Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-28 Nicholas G. Lamson, Andrew J. Pickering, Jeffrey Wyckoff, Priya Ganesh, Elizabeth A. Calle, Joelle P. Straehla, Paula T. Hammond
Drug-carrying nanoparticles are a promising strategy to deliver therapeutics into the brain, but their translation requires better characterization of interactions between nanomaterials and endothelial cells of the blood–brain barrier (BBB). Here, we use a library of 18 layer-by-layer electrostatically assembled nanoparticles (NPs) to independently assess the impact of NP core and surface materials
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Growth factors and growth factor gene therapies for treating chronic wounds Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-28 James A. Mullin, Erfan Rahmani, Kristi L. Kiick, Millicent O. Sullivan
Chronic wounds are an unmet clinical need affecting millions of patients globally, and current standards of care fail to consistently promote complete wound closure and prevent recurrence. Disruptions in growth factor signaling, a hallmark of chronic wounds, have led researchers to pursue growth factor therapies as potential supplements to standards of care. Initial studies delivering growth factors
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Differential sensitivity to hypoxia enables shape-based classification of sickle cell disease and trait blood samples at point of care Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-27 Claudy D'Costa, Oshin Sharma, Riddha Manna, Minakshi Singh, Samrat Singh, Srushti Singh, Anish Mahto, Pratiksha Govil, Sampath Satti, Ninad Mehendale, Yazdi Italia, Debjani Paul
Red blood cells (RBCs) become sickle-shaped and stiff under hypoxia as a consequence of hemoglobin (Hb) polymerization in sickle cell anemia. Distinguishing between sickle cell disease and trait is crucial during the diagnosis of sickle cell disease. While genetic analysis or high-performance liquid chromatography (HPLC) can accurately differentiate between these two genotypes, these tests are unsuitable
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Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-26 Shih-Heng Chen, Hsin-Wen Wang, Pei-Ching Yang, Shih-Shien Chen, Chia-Hsin Ho, Pei-Ching Yang, Ying-Chi Kao, Shao-Wen Liu, Han Chiu, Yu-Jie Lin, Er-Yuan Chuang, Jen-Huang Huang, Huang-Kai Kao, Chieh-Cheng Huang
The prognosis for postinjury peripheral nerve regeneration remains suboptimal. Although transplantation of exogenous Schwann cells (SCs) has been considered a promising treatment to promote nerve repair, this strategy has been hampered in practice by the limited availability of SC sources and an insufficient postengraftment cell retention rate. In this study, to address these challenges, SCs were aggregated
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Photothermal Prussian blue nanoparticles generate potent multi-targeted tumor-specific T cells as an adoptive cell therapy Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-22 Elizabeth E. Sweeney, Palak Sekhri, Nethaji Muniraj, Jie Chen, Sally Feng, Joshua Terao, Samantha J. Chin, Danielle E. Schmidt, Catherine M. Bollard, Conrad Russell Y. Cruz, Rohan Fernandes
Prussian blue nanoparticle-based photothermal therapy (PBNP-PTT) is an effective tumor treatment capable of eliciting an antitumor immune response. Motivated by the ability of PBNP-PTT to potentiate endogenous immune responses, we recently demonstrated that PBNP-PTT could be used ex vivo to generate tumor-specific T cells against glioblastoma (GBM) cell lines as an adoptive T cell therapy (ATCT). In
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A bioengineered anti-VEGF protein with high affinity and high concentration for intravitreal treatment of wet age-related macular degeneration Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-19 Chengnan Huang, Yuelin Wang, Jinliang Huang, Huiqin Liu, Zhidong Chen, Yang Jiang, Youxin Chen, Feng Qian
Intravitreal (IVT) injection of anti-vascular endothelial growth factor (anti-VEGF) has greatly improved the treatment of many retinal disorders, including wet age-related macular degeneration (wAMD), which is the third leading cause of blindness. However, frequent injections can be difficult for patients and may lead to various risks such as elevated intraocular pressure, infection, and retinal detachment
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Fibroblasts and endothelial cells interplay drives hypertrophic scar formation: Insights from in vitro and in vivo models Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-20 Yaxin Tan, Mengde Zhang, Yi Kong, Fanliang Zhang, Yuzhen Wang, Yuyan Huang, Wei Song, Zhao Li, Linhao Hou, Liting Liang, Xu Guo, Qinghua Liu, Yu Feng, Chao Zhang, Xiaobing Fu, Sha Huang
Hypertrophic scar formation is influenced by the intricate interplay between fibroblasts and endothelial cells. In this study, we investigated this relationship using in vitro and in vivo models. Clinical observations revealed distinct morphological changes and increased vascularity at pathological scar sites. Further analysis using OCTA, immunohistochemistry, and immunofluorescence confirmed the involvement
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Efficacy of catheter-based drug delivery in a hybrid in vitro model of cardiac microvascular obstruction with porcine microthrombi Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-08 Yannick Rösch, Thorald Stolte, Miriam Weisskopf, Sabrina Frey, Rob Schwartz, Nikola Cesarovic, Dominik Obrist
Microvascular obstruction (MVO) often occurs in ST-elevation myocardial infarction (STEMI) patients after percutaneous coronary intervention (PCI). Diagnosis and treatment of MVO lack appropriate and established procedures. This study focused on two major points by using an in vitro multiscale flow model, which comprised an aortic root model with physiological blood flow and a microfluidic model of
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Immunogenicity of an adjuvanted broadly active influenza vaccine in immunocompromised and diverse populations Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-08 Dylan A. Hendy, Erik S. Pena, Luis Ontiveros-Padilla, Timothy A. Dixon, Denzel D. Middleton, Grace L. Williamson, Nicole Rose Lukesh, Sean R. Simpson, Rebeca T. Stiepel, Md Jahirul Islam, Michael A. Carlock, Ted M. Ross, Eric M. Bachelder, Kristy M. Ainslie
Influenza virus outbreaks are a major burden worldwide each year. Current vaccination strategies are inadequate due to antigenic drift/shift of the virus and the elicitation of low immune responses. The use of computationally optimized broadly reactive antigen (COBRA) hemagglutinin (HA) immunogens subvert the constantly mutating viruses; however, they are poorly immunogenic on their own. To increase
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Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-07 Wei-Wen Hu, Yen-Chi Chen, Chia-Wen Tsao, Shen-Liang Chen, Chung-Yuh Tzeng
A multifunctional bioreactor was fabricated in this study to investigate the facilitation efficiency of electrical and mechanical stimulations on myogenic differentiation. This bioreactor consisted of a highly stretchable conductive membrane prepared by depositing polypyrrole (PPy) on a flexible polydimethylsiloxane (PDMS) film. The tensile deformation of the PPy/PDMS membrane can be tuned by adjusting
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Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-04 Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Cellular phenotypes and functional responses are modulated by the signals present in their microenvironment, including extracellular matrix (ECM) proteins, tissue mechanical properties, soluble signals and nutrients, and cell–cell interactions. To better recapitulate and analyze these complex signals within the framework of more physiologically relevant culture models, high throughput culture platforms
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Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-12-04 Qiao Xiong, Ting Liu, Yidie Ying, Xiaowen Yu, Ziwei Wang, Hongliang Gao, Tianhai Lin, Weihua Fan, Zhensheng Zhang, Qiang Wei, Yuqing Ge, Shuxiong Zeng, Chuanliang Xu
Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully
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Deconvolution of synthetic mRNA expression: Nucleoside chemistry alters translatability Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-28 Hanieh Moradian, Marko Schwestka, Toralf Roch, Manfred Gossen
Recent technological advances in the production of in vitro transcribed messenger RNA (IVT-mRNA) facilitate its clinical use as well as its application in basic research. In this regard, numerous chemical modifications, which are not naturally observed in endogenous mRNA, have been implemented primarily to address the issue of immunogenicity and improve its biological performance. However, recent findings
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First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-29 Xin Feng, Pengli Gao, Yabin Li, Hui Hui, Jingying Jiang, Fei Xie, Jie Tian
Increased pulmonary vascular permeability is a characteristic feature of lung injury. However, there are no established methods that allow the three-dimensional visualization and quantification of pulmonary vascular permeability in vivo. Evans blue extravasation test and total protein test of bronchoalveolar lavage fluid (BALF) are permeability assays commonly used in research settings. However, they
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Therapeutic potential of mesenchymal stem cells from human iPSC-derived teratomas for osteochondral defect regeneration Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-29 Jiseong Kim, Jin-Su Kim, Dohyun Kim, Alvin Bacero Bello, Byoung Ju Kim, Byung-Hyun Cha, Soo-Hong Lee
Human induced pluripotent stem cells (iPSCs) hold great promise for personalized medicine, as they can be differentiated into specific cell types, especially mesenchymal stem cells (MSCs). Therefore, our study sought to assess the feasibility of deriving MSCs from teratomas generated from human iPSCs. Teratomas serve as a model to mimic multilineage human development, thus enriching specific somatic
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Microfluidic chip as a promising evaluation method in assisted reproduction: A systematic review Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-24 Tong Wu, Yangyang Wu, Jinfeng Yan, Jinjin Zhang, Shixuan Wang
The aim of assisted reproductive technology (ART) is to select the high-quality sperm, oocytes, and embryos, and finally achieve a successful pregnancy. However, functional evaluation is hindered by intra- and inter-operator variability. Microfluidic chips emerge as the one of the most powerful tools to analyze biological samples for reduced size, precise control, and flexible extension. Herein, a
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Hydraulic conductivity of human cancer tissue: A hybrid study Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-23 Hooman Salavati, Pim Pullens, Charlotte Debbaut, Wim Ceelen
Elevated tumor tissue interstitial fluid pressure (IFP) is an adverse biomechanical biomarker that predicts poor therapy response and an aggressive phenotype. Advances in functional imaging have opened the prospect of measuring IFP non-invasively. Image-based estimation of the IFP requires knowledge of the tissue hydraulic conductivity (K), a measure for the ease of bulk flow through the interstitium
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Liver-tumor mimics as a potential translational framework for planning and testing irreversible electroporation with multiple electrodes Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-23 Adriana Leticia Vera-Tizatl, Regine van der Hee, Jeroen Cornelissen, Claudia Elizabeth Vera-Tizatl, Momen Abayazid, Jurgen J. Fütterer
Irreversible electroporation (IRE) has emerged as an appealing non-ionizing, non-thermal ablation therapy, independent of antineoplastic drugs. Limited but successful outcomes in IRE conducted in vivo, in small focal hepatocellular carcinomas (HCC), have been reported. Nonetheless, the electric parameters of IRE are usually delivered in an unplanned manner. This work investigates the integration of
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Advanced technologies for biomedical applications by emerging researchers in Asia-Pacific Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-19 Weiping Wang, Chenjie Xu, Jin-Wook Yoo
Translational Impact Statement Researchers from the Asia-Pacific region are making significant contributions to the progress of biomedical engineering. In this special issue, we have collected innovative research articles from emerging investigators in the Asia-Pacific region. These articles showcase the latest advancements in technology for biomedical applications, focusing on drug delivery, disease
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Circular RNA MAP2K2-modified immunosuppressive dendritic cells for preventing alloimmune rejection in organ transplantation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-13 Shuailong Li, Amal Abu Omar, Adam Greasley, Bowen Wang, Tan Ze Wang, Serina Chahal, Raj Kumar Thapa, Douglas Quan, Anton Skaro, Kexiang Liu, Xiufen Zheng
Long-term patient and graft survival has been achieved in organ transplantation but at the expense of toxic side effects that are associated with long-term use of nonspecific immunosuppressive drugs. Discovering new regulators of dendritic cells is the key for development of an ideal treatment to prevent immune rejection. We hypothesized that knockdown of circMAP2K2 induces immunosuppressive DCs and
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Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-10 Wei Wang, Liang Chen, Yuxin Zhang, Heng Wang, Dong Dong, Jingjing Zhu, Wei Fu, Tianyi Liu
Skin wound healing often leads to scar formation, presenting physical and psychological challenges for patients. Advancements in messenger RNA (mRNA) modifications offer a potential solution for pulsatile cytokine delivery to create a favorable wound-healing microenvironment, thereby preventing cutaneous fibrosis. This study aimed to investigate the effectiveness of human adipose-derived stem cells
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A landscape of recent advances in lipid nanoparticles and their translational potential for the treatment of solid tumors Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-09 Radu A. Paun, Sarah Jurchuk, Maryam Tabrizian
Lipid nanoparticles (LNPs) are biocompatible drug delivery systems that have found numerous applications in medicine. Their versatile nature enables the encapsulation and targeting of various types of medically relevant molecular cargo, including oligonucleotides, proteins, and small molecules for the treatment of diseases, such as cancer. Cancers that form solid tumors are particularly relevant for
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Self-assembly of PEG–PPS polymers and LL-37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-08 Rong Shi, Jianxiong Qiao, Quanwu Sun, Biao Hou, Bo Li, Ji Zheng, Zhenzhen Zhang, Zhenxue Peng, Jing Zhou, Bingbing Shen, Jun Deng, Xuanfen Zhang
Refractory diabetic wounds are associated with high incidence, mortality, and recurrence rates and are a devastating and rapidly growing clinical problem. However, treating these wounds is difficult owing to uncontrolled inflammatory microenvironments and defective angiogenesis in the affected areas, with no established effective treatment to the best of our knowledge. Herein, we optimized a dual functional
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Design and use of an ex vivo peripheral simulating bioreactor system for pharmacokinetic analysis of a drug coated stent Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-11-02 Danyi Chen, Colin Krinsky, Mollie Phillips, Catherine Allred, Ava Khan, Linda B. Liu, Uwe Christians, Saami K. Yazdani
Currently, there are no ex vivo systems that can model the motion of peripheral arteries and allow for the evaluation of pharmacokinetics (PK) of endovascular devices. The objective of this study was to develop a novel peripheral simulating bioreactor system to evaluate drug pharmacokinetics of stents. We utilized 3D-printed and off-the-shelf components to construct a peripheral-simulating bioreactor
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Hydrophobic ion pairing and microfluidic nanoprecipitation enable efficient nanoformulation of a small molecule indolamine 2, 3-dioxygenase inhibitor immunotherapeutic Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-28 Parisa Badiee, Michelle F. Maritz, Pouya Dehghankelishadi, Nicole Dmochowska, Benjamin Thierry
Blockade of programmed cell death-1 (PD-1) is a transformative immunotherapy. However, only a fraction of patients benefit, and there is a critical need for broad-spectrum checkpoint inhibition approaches that both enhance the recruitment of cytotoxic immune cells in cold tumors and target resistance pathways. Indoleamine 2, 3-dioxygenase (IDO) small molecule inhibitors are promising but suboptimal
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Cancer vaccines in the clinic Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-27 Morgan E. Janes, Alexander P. Gottlieb, Kyung Soo Park, Zongmin Zhao, Samir Mitragotri
Vaccines are an important tool in the rapidly evolving repertoire of immunotherapies in oncology. Although cancer vaccines have been investigated for over 30 years, very few have achieved meaningful clinical success. However, recent advances in areas such antigen identification, formulation development and manufacturing, combination therapy regimens, and indication and patient selection hold promise
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Machine learning-directed electrical impedance tomography to predict metabolically vulnerable plaques Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-20 Justin Chen, Shaolei Wang, Kaidong Wang, Parinaz Abiri, Zi-Yu Huang, Junyi Yin, Alejandro M. Jabalera, Brian Arianpour, Mehrdad Roustaei, Enbo Zhu, Peng Zhao, Susana Cavallero, Sandra Duarte-Vogel, Elena Stark, Yuan Luo, Peyman Benharash, Yu-Chong Tai, Qingyu Cui, Tzung K. Hsiai
The characterization of atherosclerotic plaques to predict their vulnerability to rupture remains a diagnostic challenge. Despite existing imaging modalities, none have proven their abilities to identify metabolically active oxidized low-density lipoprotein (oxLDL), a marker of plaque vulnerability. To this end, we developed a machine learning-directed electrochemical impedance spectroscopy (EIS) platform
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Sensor-integrated brain-on-a-chip platforms: Improving the predictive validity in neurodegenerative research Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-18 Sarah Spitz, Silvia Schobesberger, Konstanze Brandauer, Peter Ertl
Affecting millions of individuals worldwide, neurodegenerative diseases (NDDs) pose a significant and growing health concern in people over the age of 60 years. Contributing to this trend are the steady increase in the aging population coupled with a persistent lack of disease-altering treatment strategies targeting NDDs. The absence of efficient therapeutics can be attributed to high failure rates
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Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-19 Mylène de Ruijter, Paweena Diloksumpan, Inge Dokter, Harold Brommer, Ineke H. Smit, Riccardo Levato, P. René van Weeren, Miguel Castilho, Jos Malda
In articular cartilage (AC), the collagen arcades provide the tissue with its extraordinary mechanical properties. As these structures cannot be restored once damaged, functional restoration of AC defects remains a major challenge. We report that the use of a converged bioprinted, osteochondral implant, based on a gelatin methacryloyl cartilage phase, reinforced with precisely patterned melt electrowritten
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Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-18 Liyuan Zhang, Mengqiao Chi, Yue Cheng, Zhongrong Chen, Yunxia Cao, Gang Zhao
Ovarian tissue cryopreservation is considered to be the only means to preserve fertility for prepubertal girls and women whose cancer treatment cannot be postponed. However, ovarian tissues are inevitably damaged by oxidative stress during cryopreservation, which threatens follicle survival and development, and thus affects female fertility. Therefore, reducing tissue oxidative stress injury is one
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Branched poly-l-lysine for cartilage penetrating carriers Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-16 Gavin Gonzales, Jiaul Hoque, Anna Gilpin, Biswanath Maity, Stefan Zauscher, Shyni Varghese
Joint diseases, such as osteoarthritis, often require delivery of drugs to chondrocytes residing within the cartilage. However, intra-articular delivery of drugs to cartilage remains a challenge due to their rapid clearance within the joint. This problem is further exacerbated by the dense and negatively charged cartilage extracellular matrix (ECM). Cationic nanocarriers that form reversible electrostatic
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Extracellular vesicles from normal tissues orchestrate the homeostasis of macrophages and attenuate inflammatory injury of sepsis Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-14 Xinyu Ge, Qingshu Meng, Xuan Liu, Shanshan Shi, Xuedi Geng, Enhao Wang, Mimi Li, Xiaoxue Ma, Fang Lin, Qianqian Zhang, Yinzhen Li, Lunxian Tang, Xiaohui Zhou
Extracellular vesicles (EVs) exist throughout our bodies. We recently revealed the important role of intracardiac EVs induced by myocardial ischemia/reperfusion on cardiac injury and dysfunction. However, the role of EVs isolated from normal tissues remains unclear. Here we found that EVs, derived from murine heart, lung, liver and kidney have similar effects on macrophages and regulate the inflammation
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Multimodal nanoparticle-containing modified suberoylanilide hydroxamic acid polymer conjugates to mitigate immune dysfunction in severe inflammation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-14 Nhu Truong, Andrea L. Cottingham, Shruti Dharmaraj, Jacob R. Shaw, Jackline Joy Martin Lasola, Christopher C. Goodis, Steven Fletcher, Ryan M. Pearson
Excessive immune activation and immunosuppression are opposing factors that contribute to the dysregulated innate and adaptive immune responses seen in severe inflammation and sepsis. Here, a novel analog of the histone deacetylase inhibitor (HDACi), suberoylanilide hydroxamic acid (SAHA-OH), was incorporated into immunomodulatory poly(lactic acid)-based nanoparticles (iNP-SAHA) by employing a prodrug
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Curcumin/L-OHP co-loaded HAP for cGAS-STING pathway activation to enhance the natural immune response in colorectal cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-14 Yao Xiao, Guohu Guo, Huaiming Wang, Bin Peng, Yinglin Lin, Gaowen Qu, Ben Li, Zhaojun Jiang, Fan Zhang, Jiaming Wu, Min Liang
Insufficient immune cell infiltration into the tumor microenvironment (TME) greatly compromises the clinical application of immune-checkpoint inhibitors (ICIs)-based immunotherapy. Recent findings have shown that activation of the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway can enhance natural immunity and increase lymphocyte infiltration into the TME, which presents
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Next-generation agents for fluorescence-guided glioblastoma surgery Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-11 Cristina Chirizzi, Serena Pellegatta, Alessandro Gori, Jacopo Falco, Emanuele Rubiu, Francesco Acerbi, Francesca Baldelli Bombelli
Glioblastoma is a fast-growing and aggressive form of brain cancer. Even with maximal treatment, patients show a low median survival and are often subjected to a high recurrence incidence. The currently available treatments require multimodal management, including maximal safe surgical resection, followed by radiation and chemotherapy. Because of the infiltrative glioblastoma nature, intraoperative
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Mechanochemical coupling of MGF mediates periodontal regeneration Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-07 Ying Zhao, Songbai Zhang, Bo Cheng, Fan Feng, Yue Zhu, Yanli Liu, Junjun Wang, Dehui Zou, Heng Ma, Feng Xu, Min Zhang
Clinical evidence shows that the mechanical stimulation obtained from occlusion could enhance periodontal ligament (PDL) remodeling. Mechano-growth factor (MGF) is a growth factor produced specifically following mechanical stimulus Here, we aim to investigate the mechanical enhancement potential and mechanism of the MGF in PDL regeneration. In vivo study found that MGF produced from the PDL under occlusion
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Primary T-cell-based delivery platform for in vivo synthesis of engineered proteins Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-10-07 Harikrishnan Radhakrishnan, Sherri L. Newmyer, Marvin A. Ssemadaali, Harold S. Javitz, Parijat Bhatnagar
Primary T cell has been transformed into a cell-based delivery platform that synthesizes complex biologics at the disease site with spatiotemporal resolution. This broadly applicable technology can circumvent toxicities due to systemic administration of biologics that necessitates the use of high doses and may diffuse to the healthy tissues. Its clinical translation, however, has been impeded by manufacturing
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An engineered niche delineates metastatic potential of breast cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-29 Sophia M. Orbach, Christian Y. DeVaull, Elizabeth J. Bealer, Brian C. Ross, Jacqueline S. Jeruss, Lonnie D. Shea
Metastatic breast cancer is often not diagnosed until secondary tumors have become macroscopically visible and millions of tumor cells have invaded distant tissues. Yet, metastasis is initiated by a cascade of events leading to formation of the pre-metastatic niche, which can precede tumor formation by a matter of years. We aimed to distinguish the potential for metastatic disease from nonmetastatic
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Personalized additive manufacturing of devices for the management of enteroatmospheric fistulas Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-26 Francisco José Calero Castro, Andrés Padillo Eguía, Virginia Durán Muñoz-Cruzado, Luis Tallón Aguilar, José Tinoco González, Imán Laga, Fernando de la Portilla de Juan, Felipe Pareja Ciuró, Javier Padillo Ruiz
Additive manufacturing techniques allow the customized design of medical devices according to the patient's requirements. Enteroatmospheric fistula is a pathology that benefits from this personalization due to its extensive clinical variability since the size and morphology of the wound differ extensively among patients. Standard prosthetics do not achieve proper isolation of the wound, leading to
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Rapid, label-free enrichment of lymphocytes in a closed system using a flow-through microfluidic device Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-25 Anton Mukhamedshin, Riley C. Reddington, Mai T. P. Dinh, Kumar Abhishek, Mubasher Iqbal, Marc Manheim, Sean C. Gifford, Sergey S. Shevkoplyas
The majority of adoptive cellular therapies are produced from peripheral mononuclear cells obtained via leukapheresis and further enriched for the cells of interest (e.g., T cells). Here, we present a first-of-its-kind closed system, which effectively removes ~85% of monocytes and ~88% of platelets, while recovering ~88% of concentrated T cells in a separate output stream, as the leukapheresis sample
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PEGylated therapeutics in the clinic Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-22 Yongsheng Gao, Maithili Joshi, Zongmin Zhao, Samir Mitragotri
The covalent attachment of polyethylene glycol (PEG) to therapeutic agents, termed PEGylation, is a well-established and clinically proven drug delivery approach to improve the pharmacokinetics and pharmacodynamics of drugs. Specifically, PEGylation can improve the parent drug's solubility, extend its circulation time, and reduce its immunogenicity, with minimal undesirable properties. PEGylation technology