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SIAH1 promotes the pyroptosis of cardiomyocytes in diabetic cardiomyopathy via regulating IκB-α/NF-κB signaling Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-02-01 Jinbin Wu, Yaoming Yan
Inflammation-mediated dysfunction of cardiomyocytes is the main cause of diabetic cardiomyopathy (DCM). The present study aimed to investigate the roles of SIAH1 in DCM. RT-qPCR was conducted to detect mRNA levels. ELISA was performed to detect cytokine release. Western blot was used to detect protein expression. LDH assay was used to determine cytotoxicity. In vitro ubiquitination assay was applied
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SLC7A2-mediated lysine catabolism inhibits immunosuppression in triple negative breast cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-02-01 Yuanyuan Sun, Yaqing Li, Chengying Jiang, Chenying Liu, Yuanming Song
Breast cancer is one of the most common malignancy worldwide. SLC7A2 is abnormally expressed in multi-type cancers. However, the potentials of SLC7A2 in tripe negative breast cancer (TNBC) are still unclear. This study aimed to investigate the roles of SLC7A2 and the underlying molecular mechanisms. mRNA expression was detected by RT-qPCR. The release of cytokines was detected using ELISA. Protein
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SIAH2-mediated degradation of ACSL4 inhibits the anti-tumor activity of CD8+ T cells in hepatocellular carcinoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-02-01 Fangzheng Shu, Yuhua Shi, Xiangxiang Shan, Wenzhang Zha, Rengen Fan, Wanjiang Xue
SIAH2 function as an oncogene in various cancer. However, the roles of SIAH2 in hepatocellular carcinoma (HCC) is still unknown. This study aimed to investigate the roles of SIAH2 in HCC. Immunohistochemistry was used determine SIAH2 and ACSL4 expression in clinical samples. RT-qPCR was used to determine mRNA expression. Western blot assay was applied for determined protein expression. Ubiquitination
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RBM15-mediated N6-methyl adenosine (m6A) modification of EZH2 drives the epithelial-mesenchymal transition of cervical cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-02-01 Ruixue Wang, Wenhua Tan
RBM15 functions as an oncogene in multi-type cancers. However, the report on the roles of RBM15 in cervical cancer is limited. The purpose of this study was to investigate the potentials of RBM15 in cervical cancer. RT-qPCR was conducted to determine mRNA levels. Western was carried out to detect protein expression. CCK-8 and colony formation assays were carried out to determine cell proliferation
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microRNA-486-5p Regulates DNA Damage Inhibition and Cisplatin Resistance in Lung Adenocarcinoma by Targeting AURKB Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Gaozhong Sun, Kewei Ni, Jian Shen, Dongdong Liu, Haitao Wang
Lung adenocarcinoma (LUAD) severely affects human health, and cisplatin (DDP) resistance is the main obstacle in LUAD treatment, the mechanism of which is unknown. Bioinformatics methods were utilized to predict expression and related pathways of AURKB in LUAD tissues, as well as the upstream regulated microRNAs. qRT-PCR assayed expression of AURKB and microRNA-486-5p. RIP and dual-luciferase experiments
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miR-26b-5p Affects the Progression of Acute Myeloid Leukemia by Regulating the USP48-Mediated Wnt/β-Catenin Pathway Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Yu Xie, Lin Tan, Kun Wu, Deyun Li, Chengping Li
Acute myeloid leukemia (AML) is a highly heterogeneous disease. Exploring the pathogenesis of AML is still an important topic in the treatment of AML. The expression levels of miR-26b-5p and USP48 were measured by qRT-PCR. The expression levels of related proteins were detected by Western blot. Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry, respectively. Coimmunoprecipitation
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Evidence-Based Storytelling for a Strategic Roadmap to Promote Cancer Prevention via Adolescent HPV Vaccination in Northern New England Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Matthew Dugan, Gary S. Stein, Jan Kirk Carney, Sheila Clifford-Bova
Vaccination rates for the human papillomavirus (HPV) among rural youth in northern New England lag those of more urbanized areas. Reasons include a lack of available medical offices, time constraints, perceptions of vaccines and HPV, and, to a smaller degree, delays caused by the COVID-19 pandemic. We have a responsibility to increase vaccinations in these communities. To do so, vaccination experts
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KDM4A-AS1 Promotes Cell Proliferation, Migration, and Invasion via the miR-4306/STX6 Axis in Hepatocellular Carcinoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Wei Cao, Yuhan Ren, Ying Liu, Guoshu Cao, Zhen Chen, Fan Wang
As a primary liver malignancy, hepatocellular carcinoma (HCC) is commonly induced by chronic liver disease and cirrhosis. Bioinformatics analysis reveals that long noncoding RNA KDM4A antisense RNA 1 (KDM4A-AS1) may be aberrantly expressed in HCC and its abnormal expression might influence prognosis in patients. We conducted this study to illustrate the functions and mechanism of KDM4A-AS1 in regulating
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HDAC1-Mediated Downregulation of NEU1 Exacerbates the Aggressiveness of Cervical Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Nanzi Xie, Sisi Mei, Changlan Dai, Wei Chen
HDAC1 functions as an oncogene in multi-type cancers. This study aimed to investigate the roles of histone deacetylase 1 (HDAC1) in cervical cancer (CC). mRNA expression was determined using reverse transcription quantitative polymerase chain reaction. The protein−protein complexes was analyzed using co-immunoprecipitation assay. The binding sites between NRF2 and NEU1 were confirmed by chromatin immunoprecipitation
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Prototype Salivary Assay for Quantification of Two Biomarkers for In Vitro Diagnosis of Endometriosis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Pietro Giulio Signorile, Sabrina Dominici, Rosa Viceconte, Alfonso Baldi
Endometriosis, a very common disease in women, is characterized by endometrial structures outside the uterine cavity. The lack of a reliable noninvasive diagnostic test and the often nonspecific symptoms of this pathology are responsible for the delay in definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers
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KRT6A Inhibits IL-1β-Mediated Pyroptosis of Keratinocytes via Blocking IL-17 Signaling Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Yuan Li, Qi Wu
Keratin 6A (KRT6A) is involved in the pathogenesis of various skin diseases. However, the reports on the roles of KRT6A in atopic dermatitis (AD) are limited. This study aimed to investigate the potentials of KRT6A in AD. mRNA levels were detected by RT-PCR. Cytokine release was determined by ELISA. Protein expression was determined using Western blot. Cell viability was determined by CCK-8. Cytotoxicity
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Pathogens in Crohn’s Disease: The Role of Adherent Invasive Escherichia coli Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Gaetano Iaquinto, Vera Rotondi Aufiero, Giuseppe Mazzarella, Angela Lucariello, Luigi Panico, Raffaele Melina, Salvatore Iaquinto, Antonio De Luca, Carmine Sellitto
n Crohn's disease (CD), gut dysbiosis is marked by the prevalence of pathogenic bacterial species. Although several microbes have been reported as risk factors or causative agents of CD, it is not yet clear which is the real trigger of the disease. Thirty years ago, a new pathovar of Escherichia coli strain was isolated in the ileal mucosa of CD patients. This strain, called adherent invasive E. coli
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DLEU2/EZH2/GFI1 Axis Regulates the Proliferation and Apoptosis of Human Bone Marrow Mesenchymal Stem Cells Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Qing Yao, Xuezhi He, Jing Wang, Juan Liu, Qing Zhang, Jie Zhang, Yawen Bo, Lin Lu
Long non-coding RNAs (lncRNAs) has become a vital regulator in the pathogenesis of osteoporosis (OP). This study aimed to investigate the role of lncRNA DLEU2 in the development of proliferation and apoptosis of human bone marrow mesenchymal stem cells (hBMSCs). High-throughput sequencing in bone tissues from 3 pairs of healthy donors and OP patients was used to search for differential lncRNAs. The
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KIF20B Correlates with LUAD Progression and Is an Independent Risk Factor Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Jianye Yang, Liang Xu, Xiaoliang Han
Objective: Kinesin family proteins (KIFs) play crucial roles in human tumorigenesis and progression. This study aimed to investigate the expression and association of Kinesin family member 20B (KIF20B) with lung adenocarcinoma (LUAD). Methods: RNA-seq data from LUAD patients (n = 535) were extracted from TCGA. KIF20B expression was compared between tumor tissues and controls, and between different
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Ectopic Expression of TIGIT in Lung Adenocarcinoma and Its Clinical Significance Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Shuyan He, Ning Qian, Shujuan Ren, Chenglong Ma, Dong Shen, Ye Tian
This study aimed to investigate the T cell immunoreceptor with ITIM and Ig domains (TIGIT) expression in lung adenocarcinoma (LUAD). TIGIT expression was measured by western blot, reverse transcription quantitative polymerase chain reaction. Seventy-two paired surgical specimens were collected from patients with stage I-IV LUAD. The expression of TIGIT in surgical specimens was determined using immunohistochemistry
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Down-Regulation of CPEB4 Alleviates Preeclampsia through the Inhibition of Ferroptosis by PFKFB3 Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Jiao Song, Hailan Yang
Gestational diabetes mellitus (GDM) complicated with preeclampsia can lead to polyhydramnios, ketosis. Herein, we explored that CPEB4 in cancer progression of preeclampsia and its underlying mechanism. All the serum samples were collected from patients with preeclampsia. These was the induction of CPEB4 in patients with preeclampsia. The serum of CPEB4 mRNA expression was positive correlation with
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Differential Expression of KIF18B in Gastric Cancer and Its Role in Chemotherapy Sensitivity Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Li-Hong Gan, Ling Yao, Jin-Hua Yan, Ya-Qin Huang, Li Zheng, Peng Liu, Ling Lei
Gastric cancer (GC) is a main cause of cancer death in the world, and improving the chemotherapy sensitivity can enhance the chemotherapy efficacy of GC. The study objective is to explore the differential KIF18B expression in GC and its effect on GC chemotherapy sensitivity. The KIF18B expression in GC tissues and adjacent normal tissues was analyzed by real-time quantitative polymerase chain reaction
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UTP23 Is a Promising Prognostic Biomarker and Is Associated with Immune Infiltration in Breast Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Jindong Li, Siman Xie, Benteng Zhang, Weiping He, Yan Zhang, Jun Wang, Li Yang
Breast cancer is one of the malignant tumors with a high incidence and mortality rate among women worldwide, and its prevalence is increasing year by year, posing a serious health risk to women. UTP23 (UTP23 Small Subunit Processome Component) is a nucleolar protein that is essential for ribosome production. As we all know, disruption of ribosome structure and function results in improper protein function
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MIR222HG/LIN28B/ATG5 Axis Drives M2 Macrophage Polarization and Proliferation of Hepatocellular Carcinoma Cells Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Xiao Zuo, Yan Shao, Yuhang Liang, Chenglong Huo, Shuai Wang
Long non-coding RNAs (lncRNAs) are involved in the pathogenesis of hepatocellular carcinoma (HCC). This study aimed to investigate the potential of MIR222HG in HCC. HCC cells were co-cultured with U937 cells. Gene expression was determined using reverse transcription-quantitative (RT-q) PCR and western blot. Functional analysis was performed using Cell Counting Kit 8 (CCK-8), colony formation, and
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Arboviruses: Transmission and Host Resistance Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-12-01 Sidra Anwar, Bilal Ahmed, Muhammad Imran Qadir
In this review, there is a complete description of the classes of arboviruses, their evolutionary process, virus characterization, disease transmission methods; it also describes about the vectors involved in transmission and their mood of transmission, both biologically as well as non-biologically and, about host, the resistance mechanism in host, and artificial methods of preventing those viral transmissions
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Methylation Regulation of LPCAT3 Improves Osteoarthritis by Regulating ACSL4 to Inhibit Chondrocyte Ferroptosis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Kaken Habaxi, Wei Wang, Maimaitiaili Taximaimaiti, Li Wang
With the increasing aging population in China, the incidence rate of knee osteoarthritis is expected to rise annually. Therefore, we conducted a study to investigate the crucial role of LPCAT3 in osteoarthritis and its underlying mechanisms. We collected samples from normal volunteers (n = 12) and patients with osteoarthritis (n = 12) at our hospital. It was observed that LPCAT3 mRNA expression was
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Pan-Cancer Analysis of the LOX Family Reveals that LOX Affects Tumor Prognosis by Affecting Immune Infiltration Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Chunlong Zheng, Yan Chu, Nian Zhang, Ting Jia, Yuanyuan Li, Tao Jiang, Jianyong Sun
The lysyl oxidase (LOX) gene family encodes for a group of copper-dependent enzymes that play a crucial role in the cross-linking of collagen and elastin fibers in the extracellular matrix (ECM). Dysregulation of LOX gene expression has been implicated in various pathological conditions, including cancer. Several studies have shown that the LOX gene family is involved in cancer progression and metastasis
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Novel Pathways between Invasiveness Modulators in Breast Cancer Single Cells Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 George L. Eliceiri
Individual cells are known to behave differently than their whole populations of cells. The present work focused on proteins that control cancer invasiveness. Individual Dicer siRNA knockdown of HER4, CDC42, and E-cadherin decreased MMP1 mRNA levels in SCP2, a cancer single-cell progeny that is highly metastatic to bone and adrenal gland. Individual knockdown of β-catenin, CDC42, HER3, and the γ catalytic
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lncRNA799/TBL1XR1/ZEB1 Axis Forms a Feedback Loop to Promote the Epithelial-Mesenchymal Transition of Cervical Cancer Cells Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Lingmin Liao, Peng Huang, Jiali Zhao, Ziying Wang, He Chen, Chunquan Zhang, Long Huang
Cervical cancer is a common malignancy among women worldwide. Long non-coding RNAs (lncRNAs) are frequently involved in the pathogenesis of cervical cancer. Therefore, the present study aimed to investigate the potentials of lncRNA799 in cervical cancer. mRNA and protein expression were detected by reverse transcription-quantitative polymerase chain reaction and Western blot analysis, respectively
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Molecular Expression and Prognostic Implications of Krüppel-Like Factor 3 (KLF3) in Clear Cell Renal Cell Carcinoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Bin Wan, Wensheng Zhang, Xinxi Deng, Yigang Lu, Zhuo Zhang, Yang Yang
A major subtype of renal cancer is clear cell renal cell carcinoma (ccRCC). Krüppel-like factor 3 (KLF3) dysfunction is also revealed leading to poor prognosis in multiple cancer types. However, dysregulation and molecular dynamics of KLF3 underlying ccRCC progression still remains elusive. Here KLF3 gene and protein expressions in ccRCC were explored using data cohorts from The Cancer Genome Atlas
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Bromodomain Proteins Epigenetically Regulate the Mitotically Associated lncRNA MANCR in Triple Negative Breast Cancer Cells Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Kirsten M. Tracy, Shannon Prior, Willem T. Trowbridge, Joseph R. Boyd, Prachi N. Ghule, Seth Frietze, Janet L. Stein, Gary S. Stein, Jane B. Lian
Long non-coding RNA (lncRNA)−mediated control of gene expression contributes to regulation of biological processes that include proliferation and phenotype, as well as compromised expression of genes that are functionally linked to cancer initiation and tumor progression. lncRNAs have emerged as novel targets and biomarkers in breast cancer. We have shown that mitotically associated lncRNA MANCR is
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Advances in the Management of Neuroblastoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Muhammad Imran Qadir, Bilal Ahmed, Sumaira Noreen
Neuroblastoma is a malignant tumor of neuroblasts, immature nerve cells found in several areas of the body. It usually affects children under age of 5. As usual, the tumor has ability to grow rapidly and to expand vastly which ultimately leads to death. Mostly, management decisions can be drawn by the prediction of the stage of the disease as well as age at the time of its diagnosis. There are four
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Multispectral Imaging of Metabolic Fluorophores: Comparing In Vivo and Fresh Ex Vivo Tissue Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Gary E. Carver, Sarah A. Locknar, Prachi N. Ghule, Christopher J. Pung, Donald L. Weaver, Janet L. Stein, Gary S. Stein
The enhanced uptake of glucose by cancer cells via aerobic glycolysis occurs when the lactic acid pathway is favored over the citric acid cycle. The lactic acid cycle in cancer cells influences the cytosolic concentration of metabolic fluorophores including NADH (the reduced form of nicotinamide adenine dinucleotide) and flavin adenine dinucleotide (FAD). In particular, the literature has shown that
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K-Homology Type Splicing Regulatory Protein: Mechanism of Action in Cancer and Immune Disorders Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Jonathan D. Leavenworth, Nabiha Yusuf, Quamarul Hassan
K homology-type splicing regulatory protein (KSRP) is emerging as a key player in cancer biology, and immunology. As a single-strand nucleic acid binding protein it functions in both transcriptional and post-transcriptional regulation, while facilitating multiple stages of RNA metabolism to affect proliferation and control cell fate. However, it must interact with other proteins to determine the fate
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Analysis of Transgenic Cotton Plants Containing Universal Stress Protein (GaUSP-1, GaUSP-2) and Zinc Finger Transcriptional Factor (GaZnF) Genes under Drought Stress Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Muhammad Hamza Basit, Bushra Rasheed, Kausar Malik, Zoha Masood, Samia Afzal
Water is the most limiting factor for plant growth and crop productivity. Drought stress adversely affects crop yield throughout the world. Up to 50% of crop yield in Pakistan is severely affected by the shortage of water. Cotton is an important cash crop for Pakistan known as "white gold." It accounts for 8.2% of the value added in agriculture and about 3.2% of GDP. Besides, being the world's fourth-largest
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miRNA-27b-3p/TPX2 Axis Regulates Clear Cell Renal Cell Carcinoma Cell Proliferation, Invasion and Migration Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-10-01 Nana Liu, Yicheng Jiang, Shiyuan Chen, Fang Pan, Yao Tang, Xingping Tan
There is a wide variety of cancer cells that can be linked to the presence of TPX2. However, there is not a lot of evidence regarding its role in the development and maintenance of clear cell renal cell carcinoma (ccRCC). In our study, bioinformatics analysis was performed to obtain differentially expressed mRNAs and miR-NAs in ccRCC. Survival curves predicted correlation of TPX2 expression with patient
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Long Noncoding RNA lnc-TCEA1-3 Affects Osteoclastic Function by Regulating ATP6V1H Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Yuzhuan Hou, Shaoqing Yang, Zanyan Zhao, Yongqing Huang, Yanli Zhang, Wenyan Ruan, Xiaohong Duan
H subunit of V-ATPase (ATP6V1H) is specifically expressed in osteoclasts and its deficiency lead to osteoporosis. Our group previously found four intronic SNPs of ATP6V1H related to reduced bone mineral density, but the mechanisms was not clear. In this study, we found that the above four SNPs were located at lncRNA lnc-TCEA1-3 by using bioinformatics analysis. We further detected the function of lnc-TCEA1-3
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Comprehensive Characterization of HATs and HDACs in Human Cancers Reveals Their Role in Immune Checkpoint Blockade Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Rong Sun, Zike Chen, Xuanhao Qu, Jie Zhang, Lehan Liu, Zhuheng Zhong, Weibing Zhang, Yihui Fan
Histone acetylation that controlled by two mutually antagonistic enzyme families, histone acetyl transferases (HATs) and histone deacetylases (HDACs), as one of major epigenetic mechanisms controls transcription and its abnormal regulation was implicated in various aspects of cancer. However, the comprehensive understanding of HDACs and HATs in cancer is still lacking. Systematically analysis through
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The ceRNA Mechanism of lncRNA MEG3/miR-21-5p/SPRY2 in Cell Proliferation and Apoptosis in Bladder Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Yangchun Hong, Zhen Li, Yixin Su, Hexian Pu, Xiuxiu Zhang
Bladder cancer (BC) is the second most common genitourinary malignancy. Long noncoding RNA (lncRNA) is implicated in BC progression. This study delved into the underlying mechanism of lncRNA MEG3 in BC. Bioinformatics analysis predicted the expression of lncRNA MEG3, its association with the survival of BC patients, its subcellular localization, and its binding sites with miR-21-5p. Differentially
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Oncolytic Virotherapy: An Advanced Microbial Approach for the Management of Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2024-01-01 Muhammad Farhan Siddiq Rao, Bilal Ahmed, Muhammad Imran Qadir
Destruction of the tumor (cancerous) cells may be caused by live viruses, which have replicative ability and replicate selectively in tumor cells, known as oncolytic virotherapy. In comparison of conservative cancer therapy, tumor-selective replicating viruses have more advantages. These viruses have introduced new methodologies for the human cancer treatment. Numerous strategies are used in development
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Long Noncoding RNA MAGI2-AS3 Represses Cell Progression in Clear Cell Renal Cell Carcinoma by Modulating the miR-629-5p/PRDM16 Axis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-08-01 Chengquan Yan, Pengfei Wang, Chaofei Zhao, Guangwei Yin, Xin Meng, Lin Li, Shengyong Cai, Bin Meng
The objective of this study was to determine the regulatory mechanism of MAGI2-AS3 in clear cell renal cell carcinoma (ccRCC), thereby supplying a new insight for ccRCC treatment. Expression data in TCGA-KIRC were obtained. Target gene lncRNA for research was determined using expression analysis and clinical analysis. lncRNA's downstream regulatory miRNA and mRNA were predicted by bioinformatics databases
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Prognosis prediction of disulfidptosis-related genes in bladder cancer and comprehensive analysis of immunotherapy Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-05-01 Yonggui Xiao, Chonghao Jiang, Danping Xu, Youlong Huili, Shiwen Nie, Hubo Li, Xiaohai Guan, Fenghong Cao
Bladder cancer is one of the three major tumors in urology, and its diagnosis and treatment is a global problem. As a newly discovered mechanism of cell death, disulfidptosis is expected to help diagnose and treat bladder cancer patients. Firstly, we obtained the data required for this study in an open database through a common process. Next, we analyzed this study through bioinformatics techniques
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BAG2 is a novel prognostic biomarker and promising immunotherapy target in uveal melanoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-05-01 Chaolin Li, Hao Shi
ABSTRACT Background: The importance of BAG2 in malignancy is gradually being recognized, however, information on its role in uveal melanoma (UVM) is limited. We aimed to elucidate its function and potential mechanism of action in UVM. Methods: Using The Cancer Genome Atlas (TCGA) and GEO-related datasets, we analyzed the differential expression of BAG2 in tumors, combined with clinical information
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Chloroquine sensitizes esophageal carcinoma EC109 cells to paclitaxel by inhibiting autopahgy Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-03-01 Zichun Yuan, Jiajing Cai, Qin Du, Qiang Ma, Lei Xu, Yan Cai, Xiaowu Zhong, Xiaolan Guo
As an autophagy inhibitor, Chloroquine (CQ) showed anti-tumor effect on several types of cancer and paclitaxel (PTX) is widely used in the treatment of esophageal carcinoma patients, but chemoresistance remains a major hurdle for PTX application due to the cytoprotective autophagy. Therefore, the aim of this study was to investigate whether CQ could elevate the anti-tumor effect of PTX on esophageal
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Construction of miRNA–mRNA Regulatory Network to identify potential biomarkers in Infantile hemangioma by integrated Bioinformatics Analysis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-03-01 LiaoBin Chen, Boming Zhao, Bin Li
Introduction Infantile hemangioma (IH) is the most common vascular tumor among infants and children. However, the understanding of pathogenesis about IH has not been fully elucidated, and the potential diagnostic maker remains further explored. In this study, we aimed to find miRNAs as potential biomarkers of IH through bioinformatic analysis. Methods The microarray datasets GSE69136, GSE100682 were
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Long non-coding RNA LPP-AS2 plays an anti-tumor role in thyroid carcinoma by regulating the miR-132-3p/OLFM1 axis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-03-01 Bowei Zhang, Tong Liu, Yi Gu, Li Ren, Jinju Wang, Chao Feng, Zhe Song
Background: The cancer-promoting function of the long non-coding RNA (lncRNA) LPP-AS2 has been documented in different cancers. Nonetheless, its role in thyroid carcinoma (THCA) remains unestablished. Methods: RT-qPCR and Western blotting were conducted to estimate the expressions of lncRNA LPP-AS2, miR-132-3p, and OLFM1. The THCA cells’ functions were assessed through CCK8 assays, Transwell invasion
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TPP1 Inhibits DNA Damage Response and Chemosensitivity in Esophageal Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Jilin Wen, Xiaowu Zhong, Chuanli Gao, Miyuan Yang, Maoju Tang, Zichun Yuan, Qin Wang, Lei Xu, Qiang Ma, Xiaolan Guo, Li Fang
TPP1, as one of the telomere-protective protein complex, functions to maintain telomere stability. In this study, we found that TPP1 was significantly upregulated in esophageal cancer (EC). We found that the proliferation and migration ability were significantly inhibited, while the results of flow cytometry assay indicated that the growth was hindered in the G1 phase after TPP1 knockdown. However
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A Pan-Cancer Analysis to Provide Insight into the Immunological Role and Prognostic Value of HTRA3 Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Qi Xu, Jiale Wang, Jing Wang, Yukuan Zhu, Baihui Chen, Runzhao Guo, Yao Qian, Haiyu Yuan, Feng Liu
High-temperature requirement factor A3 (HTRA3), a member of the HTRA protein family, is closely associated with apoptosis and plays a crucial role in controlling signal transmission and cancer development. However, the regulatory pathways of HTRA3 in tumors are not fully understood, necessitating a comprehensive analysis of HTRA3 in cancers. In this study, we conducted a multi-omics analysis of HTRA3
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N6-Methyladenosine Modification and Prognostic Analysis of UBE2K in Hepatocellular Carcinoma: A Potential Target Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Jinhui Guo, Jie Zhao
The treatment of hepatocellular carcinoma (HCC) is still relatively lacking, the prognosis is poor, and the potential mechanism of carcinogenesis has not been thoroughly studied. In this study, Ubiquitin-conjugating enzyme E2K (UBE2K) transcript levels in HCC patients were up-regulated in two databases, GEO and TCGA. External validation was performed using Western blot experiments. Compared to normal
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ZCCHC4 Promotes Osteosarcoma Progression by Upregulating ITGB1 Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Lingli Luo, Xiaojun Tang, Linghua Liu, Guojun Tang, Liangyuan Chen, Guiping Chang, Zhihong Xiao
Zinc finger CCHC-type containing 4 (ZCCHC4), RNA binding protein, has been reported to mediate rRNA methylation and affect tumor cell proliferation. However, the role of ZCCHC4 in the regulation of osteosarcoma (OS) remains unknown. ZCCHC4 was highly expressed in OS tissues and cell lines. Overexpression or silencing of ZCCHC4 promoted or inhibited cell proliferation, epithelial-mesenchymal transition
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Identification of a Diagnosis and Therapeutic Inflammatory Response-Related Gene Signature Associated with Esophageal Adenocarcinoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Yang Xie, Jun Li, Qing Tao, Chunyan Zeng, Youxiang Chen
The purpose of this study is to identify the key regulatory genes related to the inflammatory response of esophageal adenocarcinoma (EAC) and to find new diagnosis and therapeutic options. We downloaded the dataset GSE72874 from the Gene Expression Omnibus database for this study. Weighted gene co-expression network analysis (WGCNA) and differentially expressed genes (DEGs) analysis were used to find
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Identification and Validation of Key Gene Modules and Pathways in Coronary Artery Disease Development and Progression Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Ewnji Yoon, Wenjing Zhang, Yunpeng Cai, Changnong Peng, Daxin Zhou
The development and progression of atherosclerosis represent a chronic process involving complex molecular interactions. Therefore, identifying the potential hub genes and pathways contributing to coronary artery disease (CAD) development is essential for understanding its underlying molecular mechanisms. To this end, we performed transcriptome analysis of peripheral venous blood collected from 100
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lncRNA EGFEM1P Drives the Progression of Papillary Thyroid Cancer by Regulating miR-6867-5p/CHI3L1 Axis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Zhanwu Ma, Guoxian Wang, Lin Hu
Long noncoding RNA (lncRNA), a subgroup of noncoding RNA with > 200 nt, plays critical roles in cancer progression. Here, we aimed to explore the detailed biological function of lncRNA EGFEM1P during papillary thyroid cancer (PTC) progression. RT-qPCR and Western blot were used to analyze the expression of lncRNA EGFEM1P, miR-6867-5p, and CHI3L1. CCK8, colony formation, and Transwell migration assays
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Raising the HPV Vaccination Rate in Rural Northern New England Using Local Opinion Leaders Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Matthew Dugan, Gary S. Stein, Shamima Khan, Sheila Clifford-Bova Clifford-Bova, Finlay Pilcher, Jan Kirk Carney
The human papillomavirus is associated with a range of cancers. A vaccine introduced in 2006 has dramatically decreased the incidence of these cancers, but Americans still experience over 47,000 new cases of HPV-related cancers each year. The situation is worse in rural areas, where vaccination rates lag the national average, making HPV a significant health disparity issue. This article lays out an
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AIDS Dementia Complex: Neurotoxicity in AIDS Patients Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Muhammad Imran Qadir, Bilal Ahmed, Sumaira Noreen
AIDS dementia complex (ADC) is a nervous system disorder that harms the neurons in different parts of the brain. Various features are involved in altering the normal activities of neurons. Neurotoxicity is induced due to HIV viral proteins such as gp120, SDF, Tat, etc. These proteins target macrophages, glial cells, astrocytes, and release neurotoxins. These neurotoxins proved harmful for the neurons
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Changes of Oral Flora, Inflammatory Factors, and Immune Function Indicators in Patients with Chronic Periodontitis and Their Clinical Significance Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Guodong Jia, Zhenhu Ren
This research focuses on investigating the changes of oral flora, inflammatory factors, and immune function indicators in patients with chronic periodontitis (CP) and their clinical significances. Clinical indices such as gingival index (GI) and sulcus bleeding index (SBI) of the study subjects were recorded. The levels of oral flora, inflammatory factors and T lymphocyte subsets in gingival crevicular
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Exosome-Mediated lncRNA LINC01140 Attenuates Breast Cancer Progression by Regulating the Wnt/β-Catenin Pathway Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Zhe Guo, Yang Li, Wenhuan Li, Hai Li, Zhiyong Wu
Exosome-delivered long non-coding RNAs have a role in the cancer control. It is unknown how exosomal LINC01140 contributes to the breast cancer (BC) growth. The purpose of this investigation is to identify exosomal LINC01140's function in the development of breast cancer. Using quantitative reverse transcripion polymerase chain reaction, the expression of LINC01140 was measured. To investigate how
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linc00511 Knockdown Inhibits Lung Cancer Progression by Regulating miR-16-5p/MMP11 Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Zhengyi Song, Jing Luo, Ming Wu, Zelin Zhang
Lung cancer (LC) is a malignant tumor that extremely impairs people. According to numerous studies, long non-coding RNA (lncRNA) was inextricably involved in the advancement of LC. The work aspired to identify linc00511 expression in LC and to dig for the underlying mechanisms linc00511 regulated LC progression. Experimental outcomes revealed that linc00511 was obviously upregulated in LC, and linc00511
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System Analysis Based on Pancreatic Cancer Progression Identifies BRINP2 as a Novel Prognostic Biomarker Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Yixing Kang, Xiangwen Xu, Jikui Liu
Pancreatic adenocarcinoma (PAAD) is a malignant tumor of the digestive system, which develops rapidly and has no obvious early symptoms. This study aims to discover the biomarkers associated with PAAD development. We obtained RNA expression of PAAD patient samples and corresponding clinical data from The cancer genome atlas (TCGA), and screened out BMP/RA-inducible neural-specific protein 2 (BRINP2)
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Adeno-Associated Virus−Mediated Gene Therapy Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Hassan Zaman, Aakif Khan, Khalid Khan, Shazma Toheed, Muhammad Abdullah, Hafiz Muhammad Zeeshan, Abdul Hameed, Muhammad Umar, Muhammad Shahid, Kausar Malik, Samia Afzal
Choice of vector is the most critical step in gene therapy. Adeno-associated viruses (AAV); third generation vectors, are getting much attention of scientists to be used as vehicles due to their non-pathogenicity, excellent safety profile, low immune responses, great efficiency to transduce non-dividing cells, large capacity to transfer genetic material and long-term expression of genetic payload.
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Construction of miRNA-mRNA Regulatory Network to Identify Potential Biomarkers in Infantile Hemangioma by Integrated Bioinformatics Analysis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Boming Zhao, Bin Li, Jun Zhang, Yongjian Qi, Biao Chen, Liaobin Chen
Infantile hemangioma (IH) is the most common vascular tumor among infants and children. However, the understanding of pathogenesis about IH has not been fully elucidated, and the potential diagnostic maker remains further explored. In this study, we aimed to find miRNAs as potential biomarkers of IH through bioinformatic analysis. The microarray datasets GSE69136, GSE100682 were downloaded from the
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Long Non-Coding RNA LPP-AS2 Plays an Anti-Tumor Role in Thyroid Carcinoma by Regulating the miR-132-3p/OLFM1 Axis Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Bowei Zhang, Tong Liu, Yi Gu, Li Ren, Jinju Wang, Chao Feng, Zhe Song
The cancer-promoting function of the long non-coding RNA (lncRNA) LPP-AS2 has been documented in different cancers. Nonetheless, its role in thyroid carcinoma (THCA) remains unestablished. Reverse transcription quantitative polymerase chain reaction and Western blotting were conducted to estimate the expressions of lncRNA LPP-AS2, miR-132-3p, and OLFM1. The THCA cells' functions were assessed through
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Sympathetic Activation Promotes Cardiomyocyte Apoptosis in a Rabbit Susceptibility Model of Hyperthyroidism-Induced Atrial Fibrillation via the p38 MAPK Signaling Pathway Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Jialin Zheng, Shijian Zhao, Qishi Yang, Yantao Wei, Jianmei Li, Tao Guo
Excess thyroid hormone secretion can cause endocrine metabolic disorders, which can lead to cardiovascular diseases, including heart enlargement, atrial fibrillation (AF), and heart failure. The present study investigated the molecular mechanisms of hyperthyroidism-induced AF. A rabbit susceptibility model of hyperthyroidism-induced AF was constructed, and metoprolol treatment was administered. Norepinephrine
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CCT3 as a Diagnostic and Prognostic Biomarker in Cervical Cancer Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Man Li, Jianmin Zeng, Yuhuan Chang, Lili Lv, Guoliu Ye
The chaperonin-containing TCP1 complex subunit 3 (CCT3) has been reported to be involved in the development and prognosis of many tumors, including cervical cancer (CC). This study aimed to analyze the expression and prognostic value of CCT3 in CC by bioinformatics and retrospective study. CCT3 gene expression profiles and clinical information in CC were downloaded from the cancer genome atlas (TCGA)
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Analysis of Angiogenesis-Related Signatures in the Tumor Immune Microenvironment and Identification of Clinical Prognostic Regulators in Lung Adenocarcinoma Crit. Rev. Eukaryot. Gene Expr. (IF 1.6) Pub Date : 2023-01-01 Qing Zhou, Xi Chen, Qiuyan Chen, Lu Hao
Tumor angiogenesis is considered to be an important part of the mechanism of tumor progression and metastasis, and its specific function in lung adenocarcinoma has not been fully studied. In this study, we used the transcriptome and genome data of lung adenocarcinoma patients to analyze the expression of 36 angiogenesis regulators in lung adenocarcinoma. Consensus clustering analysis divided lung adenocarcinoma