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Uncovering the presence of CVPD disease in citrus varieties of South Sulawesi, Indonesia: A molecular approach J. Genet. Eng. Biotechnol. Pub Date : 2024-02-27 Mustika Tuwo, Tutik Kuswinanti, Andi Nasruddin, Elis Tambaru
The citrus vein phloem degeneration (CVPD) disease is one of important diseases that infects citrus plants and threatens global citrus production and quality due to its severe symptoms and rapid spread. In the 2000s, South Sulawesi Province as one of the citrus producers in Indonesia reported CVPD infection. To date, it is still uncertain as to whether the citrus production center has already been
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Enhancing in vitro regeneration via somatic embryogenesis and Fusarium wilt resistance of Egyptian cucumber (Cucumis sativus L.) cultivars J. Genet. Eng. Biotechnol. Pub Date : 2024-02-23 Hamdy M. Hamza, Rana H. Diab, Ismael A. Khatab, Reda M. Gaafar, Mohamed Elhiti
Somatic embryogenesis offers a reliable method for cucumber ( L.) regeneration and genetic enhancement against Fusarium wilt. This study aimed to establish a tailored somatic embryogenesis system for Egyptian cultivars, fostering genetic improvements and Fusarium wilt-resistance lines. Employing the Optimal Arbitrary Design (OAD) approach, we optimized the induction medium, initiating prolific embryogenic
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Genetic diversity and population structure of natural provenances of Sonneratia caseolaris in Vietnam J. Genet. Eng. Biotechnol. Pub Date : 2024-02-23 Son Le, Thanh Van Le
is considered the most important mangrove species for reforestation and conservation programs. Therefore, the knowledge of genetic diversity and the population structure of the species has important implications both for the conservation of existing genetic resources and development programs. In the present study, the genetic diversity and structure population of eight populations of from the Northern
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Human exons and introns classification using pre-trained Resnet-50 and GoogleNet models and 13-layers CNN model J. Genet. Eng. Biotechnol. Pub Date : 2024-02-21 Feriel Ben Nasr Barber, Afef Elloumi Oueslati
Examining functions and characteristics of DNA sequences is a highly challenging task. When it comes to the human genome, which is made up of exons and introns, this task is more challenging. Human exons and introns contain millions to billions of nucleotides, which contributes to the complexity observed in this sequences. Considering how complicated the subject of genomics is, it is obvious that using
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Foliar application of plant-derived peptides decreases the severity of leaf rust (Puccinia triticina) infection in bread wheat (Triticum aestivum L.) J. Genet. Eng. Biotechnol. Pub Date : 2024-02-15 Urbashi Panthi, Brent McCallum, Igor Kovalchuk, Christof Rampitsch, Ana Badea, Zhen Yao, Andriy Bilichak
Screening and developing novel antifungal agents with minimal environmental impact are needed to maintain and increase crop production, which is constantly threatened by various pathogens. Small peptides with antimicrobial and antifungal activities have been known to play an important role in plant defense both at the pathogen level by suppressing its growth and proliferation as well as at the host
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Characterization of phenylalanine ammonia lyase and revealing flavonoid biosynthesis in Gymnema sylvestre R. Br through transcriptomic approach J. Genet. Eng. Biotechnol. Pub Date : 2024-02-13 Kuldeepsingh A. Kalariya, Ravina R. Mevada, Manish Das
R.Br. is famous medicinal plant among diabetics for its gymnemic acid content. It also contains flavonoids, which are an essential component in various other products. Though some molecular information on the biosynthesis of gymnemic acid, polyoxypregnane, micro RNAs and photosynthetic efficiency is available, there is no gene level information available on the biosynthesis of flavonoids in this plant
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Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha J. Genet. Eng. Biotechnol. Pub Date : 2024-02-12 Sheila Chairunnisa, Apon Zaenal Mustopa, Budiman Bela, Moh Egy Rahman Firdaus, Shasmita Irawan, Rosyida Khusniatul Arifah, Herman Irawan, Maritsa Nurfatwa, Rifqiyah Nur Umami, Nurlaili Ekawati, Ai Hertati, Nurhasni Hasan
Human papillomavirus (HPV) vaccination is one of the crucial national vaccination programs aimed at reducing the prevalence of the diseases associated with HPV infections, which continue to pose a global health concern. However, a significant disparity exists in the distribution of HPV vaccine, particularly in low-middle income countries where the cost of HPV vaccine becomes a major obstacle. Thus
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Multi-epitope vaccine design against leishmaniasis using IFN-γ inducing epitopes from immunodominant gp46 and gp63 proteins J. Genet. Eng. Biotechnol. Pub Date : 2024-02-02 Amir Dehghani, Mina Mamizadeh, Atena Karimi, Seyyed Amir Hosseini, Davood Siamian, Morteza Shams, Shadan Ghiabi, Gholam Basati, Amir Abaszadeh
There is no currently approved human vaccine against leishmaniasis. Utilization of immunogenic antigens and their epitopes capable of enhancing immune responses against leishmaniasis is a crucial step for rational vaccine design. The objective of this study was to generate and evaluate a potential vaccine candidate against leishmaniasis, designed by immunodominant proteins from gp46 and gp63 of , which
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Bioinformatics approach for prediction and analysis of the Non-Structural Protein 4B (NSP4B) of the Zika virus J. Genet. Eng. Biotechnol. Pub Date : 2024-02-02 Mohamed E. Hasan, Aya Samir, Magdy M. Khalil, Medhat W. Shafaa
The Nonstructural Protein (NSP) 4B of Zika virus of 251 amino acids from (ZIKV/Human/POLG_ZIKVF) with accession number , Induces the production of Endoplasmic Reticulum ER-derived membrane vesicles, which are the sites of viral replication. To understand the physical basis of how proteins fold in nature and to solve the challenge of protein structure prediction, Ab-initio and comparative modeling are
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Preserving the adaptive salt stress response activity of a tissue-specific promoter with modulating activity J. Genet. Eng. Biotechnol. Pub Date : 2024-02-01 Elham R.S. Soliman
The “Redox Responsive Transcription Factor1” () promoter is transiently activated by salt stress in roots over 6 h period, followed by an adaptation phase during which its activity returns to baseline levels, even if the salt stress is prolonged. This enables the short-term production of genes that, while initially advantageous to the plant, will have long-term detrimental effects if expressed at high
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Efficacy of Event MON 87460 in drought-tolerant maize hybrids under optimal and managed drought-stress in eastern and southern africa J. Genet. Eng. Biotechnol. Pub Date : 2024-02-01 Caleb O. Obunyali, Kiru Pillay, Barbara Meisel, Eric N. Ndou, Kingstone Mashingaidze, Julius Pyton Sserumaga, Godfrey Asea, Murenga Mwimali, Regina Tende, Yoseph Beyene, Stephen Mugo, Emmanuel Okogbenin, Sylvester O. Oikeh
Frequent drought events due to climate change have become a major threat to maize ( L.) production and food security in Africa. Genetic engineering is one of the ways of improving drought tolerance through gene introgression to reduce the impact of drought stress in maize production. This study aimed to evaluate the efficacy of Event MON 87460 (; ) gene in more than 120 conventional drought-tolerant
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Role of gene interactions in the pathophysiology of skeletal dysplasias: A case report in Colombia J. Genet. Eng. Biotechnol. Pub Date : 2024-02-01 Nathalie Yepes Madrid, Lina Johanna Moreno Giraldo
Genome association studies have shown that gene-gene interactions or epistasis play a crucial role in identifying the etiology, prognosis, and treatment response of many complex diseases beyond their main effects. Skeletal dysplasias are a heterogeneous group of congenital bone and cartilage disorders with a genetic and gen-gen interaction etiology. The current classification of skeletal dysplasias
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Phylogenetic and genetic variation of common mudskippers (Periophthalmus kalolo Lesson, 1831) from the southern coast of Java, Indonesia inferred from the COI mitochondrial gene J. Genet. Eng. Biotechnol. Pub Date : 2024-02-01 Tuty Arisuryanti, Katon Waskito Aji, Faizah Nur Shabrina, Diana Febriyanti, Budi Setiadi Daryono, Dwi Sendi Priyono
The common mudskipper ( Lesson, 1831) belongs to a group of fish species that exhibit amphibious lifestyles during specific daily periods. However, identifying this species poses a challenge due to its morphological similarities with other mudskipper species. These similarities have occasionally caused misidentifications of mudskippers. In Indonesia, previous studies have examined the genetic variation
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Aggrecan-related bone disorders; a novel heterozygous ACAN variant associated with spondyloepimetaphyseal dysplasia expanding the phenotypic spectrum and review of literature J. Genet. Eng. Biotechnol. Pub Date : 2024-01-30 Hoda A. Ahmed, R. Elhossini, M. Aglan, Khalda Amr
Spondyloepimetaphyseal dysplasias (SEMD) are a large group of skeletal disorders represented by abnormalities of vertebrae in addition to epiphyseal and metaphyseal areas of bones. Several genes have been identified underlying different forms. gene mutations were found to cause Aggrecan-related bone disorders (spondyloepimetaphyseal dysplasias,spondyloepiphyseal dysplasias, familial osteochondritis
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Identification of cuproptosis-related lncRNAs signature for predicting the prognosis in patients with kidney renal clear cell carcinoma J. Genet. Eng. Biotechnol. Pub Date : 2024-01-30 Ya He, Hongxia Zhang, Jingang Li, Hui Zhou, Fei Wang, Guangliang Zhang, Yuetao Wen
Kidney renal clear cell carcinoma (KIRC), with low survival rate, is the most frequent subtype of renal cell carcinoma. Recently, more and more studies indicate that cuproptosis-related genes (CRGs) and long non-coding RNAs (lncRNAs) play a vital role in the occurrence and development of many types of cancers. However, the roles of cuproptosis-related lncRNAs (CRlncRNAs) in the KIRC was uncertain.
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Elucidating the evolution of monkeypox virus genomes through phylo-geo-network and haplogroup analysis J. Genet. Eng. Biotechnol. Pub Date : 2024-01-28 Taslima Nasrin, Md Samim Hassan, Muzaffar Iqbal, Amar Yousif, Mehboob Hoque, Nemat Ali, Safdar Ali
As the world settles down from the COVID-19 pandemic, many countries are faced with an unexpected outbreak of monkeypox infection. Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is an enveloped, double stranded DNA virus belonging to the Poxviridae family. Presently, we construct and analyze the phylo-geo-network and the corresponding haplogroups. Presently, we performed the
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Meta-analysis of biodynamic (BD) preparations reveal the bacterial population involved in improving soil health, crop yield and quality J. Genet. Eng. Biotechnol. Pub Date : 2024-01-24 Supriya Vaish, Sumit K. Soni, Balvindra Singh, Neelima Garg, Iffat Zareen Ahmad, Muthukumar Manoharan, Ajaya Kumar Trivedi
Bacterial community found in biodynamic preparations (BD500–BD507) can help improve soil health, plant development, yield, and quality. The current work describes a metagenomic investigation of these preparations to identify the bacterial communities along with the functional diversity present within them. Metagenome sequencing was performed using the Illumina MiSeq platform, which employs next-generation
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Screening of miRNAs as prognostic biomarkers and their associated hub targets across Hepatocellular carcinoma using survival-based bioinformatics approach J. Genet. Eng. Biotechnol. Pub Date : 2024-01-24 Prithvi Singh, Rubi Solanki, Alvea Tasneem, Simran Suri, Harleen Kaur, Sapna Ratan Shah, Ravins Dohare
The hepatocellular carcinoma (HCC) incident rate is gradually increasing yearly despite all the research and efforts taken by scientific communities and governing bodies. Approximately of all liver cancer cases belong to HCC. Usually, HCC patients approach the treatment in the late stages of this malignancy which becomes the primary cause of high mortality rate. The knowledge about molecular pathogenesis
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Subtractive genomics study of Xanthomonas oryzae pv. Oryzae reveals repurposable drug candidate for the treatment of bacterial leaf blight in rice J. Genet. Eng. Biotechnol. Pub Date : 2024-01-23 Ishtiaque Ahammad, Tabassum Binte Jamal, Anika Bushra Lamisa, Arittra Bhattacharjee, Nayeematul Zinan, Md. Zahid Hasan Chowdhury, Shah Mohammad Naimul Islam, Kazi Md. Omar Faruque, Zeshan Mahmud Chowdhury, Mohammad Uzzal Hossain, Keshob Chandra Das, Chaman Ara Keya, Md Salimullah
pv. is a plant pathogen responsible for causing one of the most severe bacterial diseases in rice, known as bacterial leaf blight that poses a major threat to global rice production. Even though several experimental compounds and chemical agents have been tested against pv. , still no approved drug is available. In this study, a subtractive genomic approach was used to identify potential therapeutic
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The role of zygotic genome activation in genetic−related reproductive medicine: Technological perspective, religious and bioethical concerns, challenges and benefits J. Genet. Eng. Biotechnol. Pub Date : 2024-01-23 Nameer Hashim Qasim, Abzal Zhumagaliuly, Rabiga Khozhamkul, Fakher Rahim
Zygotic Genome Activation (ZGA) is a crucial developmental milestone in early embryogenesis, marking the transition from maternal to embryonic control of development. This process, which varies in timing across species, involves the activation of the embryonic genome, paving the way for subsequent cell differentiation and organismal development. Recent advances in genomics and reproductive medicine
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Unveiling the structure and interactions of SOG1, a NAC domain transcription factor: An in-silico perspective J. Genet. Eng. Biotechnol. Pub Date : 2024-01-23 Kalyan Mahapatra
SOG1 is a crucial plant-specific NAC domain family transcription factor and functions as the central regulator of DNA damage response, acting downstream of ATM and ATR kinases. In this study, various approaches have been employed for the characterization of SOG1 transcription factor in a comparative manner with its orthologues from various plant species. Amino acid sequences of more than a hundred
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Emergence of carbapenem resistant gram-negative pathogens with high rate of colistin resistance in Egypt: A cross sectional study to assess resistance trends during the COVID-19 pandemic J. Genet. Eng. Biotechnol. Pub Date : 2024-01-23 Fatma A. Afify, Ahmed H. Shata, Nirmeen Aboelnaga, Dina Osama, Salma W. Elsayed, Nehal A. Saif, Shaimaa F. Mouftah, Sherine M. Shawky, Ahmed A. Mohamed, Omar Loay, Mohamed Elhadidy
The current study investigated the temporal phenotypic and genotypic antimicrobial resistance (AMR) trends among multi-drug resistant and carbapenem-resistant , and recovered from Egyptian clinical settings between 2020 and 2021. Bacterial identification and antimicrobial sensitivity of 111 clinical isolates against a panel of antibiotics were performed. Molecular screening for antibiotic resistance
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AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Desouky Abd-El-Haleem
The objective of this study was to identify genes associated with the biodegradation of phenol by sp. strain DF4 through the use of differential display (DD) methodology. The bacteria were grown in YEPG medium, and total RNA was extracted and analyzed using labeled primers to detect gene expression differences. Three distinctively expressed cDNA bands (ph1, ph2, and ph3) were identified, cloned, and
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The in-silico study of the structural changes in the Arthrobacter globiformis choline oxidase induced by high temperature J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Sonia Kaushik, Rashmi Rameshwari, Shilpa S. Chapadgaonkar
Choline oxidase, a flavoprotein, is an enzyme that catalyzes the reaction which converts choline into glycine betaine. Choline oxidase started its journey way back in 1933. However, the impact of the high temperature on its structure has not been explored despite the long history and availability of its crystal structure. Both choline oxidase and its product, glycine betaine, have enormous applications
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SARS-CoV-2 Omicron Spike shows strong binding affinity and favourable interaction landscape with the TLR4/MD2 compared to other variants J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Chiranjib Chakraborty, Bidyut Mallick, Manojit Bhattacharya, Siddappa N. Byrareddy
Emergences of SARS-CoV-2 variants have made the pandemic more critical. Toll-like receptor 4 (TLR4) recognizes the molecular patterns of pathogens and activates the production of proinflammatory cytokines to restrain the infection. We have identified a molecular basis of interaction between the Spike and TLR4 of SARS-CoV-2 and its present and past VOCs (variant- of concern) through analysis. The interaction
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Assessment of vitamin D status and vitamin D receptor polymorphism in Egyptian children with Type 1 diabetes J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Eman A. Mostafa, Maha M.A. Abo Hashish, Nagwa Abdallah Ismail, Hasanin M. Hasanin, Rasha M. Hasanin, Aliaa Ahmed Wahby, Ingy Ashmawy, Shereen Hamdy Abd El Aziz, Mai Magdy Abdel Wahed
The endocrine system of vitamin D regulates about 3 % of the human genome. Vitamin D exerts its actions via a nuclear vitamin D receptor (VDR) which in turn regulates insulin secretion from the pancreas. VDR gene polymorphisms could have an impact on how autoimmune illnesses like Type 1 diabetes mellitus (T1DM) develop. We aimed to explore the relation between T1DM and VDR gene polymorphisms in Egyptian
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Phytocompounds-based therapeutic approach: Investigating curcumin and green tea extracts on MCF-7 breast cancer cell line J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Radwa M. Fawzy, Amal A. Abdel-Aziz, Khalid Bassiouny, Aysam M. Fayed
Breast cancer (BC) has transcended lung cancer as the most common cancer in the world. Due to the disease's aggressiveness, rapid growth, and heterogeneity, it is crucial to investigate different therapeutic approaches for treatment. According to the World Health Organization (WHO), Plant-based therapeutics continue to be utilized as safe/non-toxic complementary or alternative treatments for cancer
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Identification of potential antiviral compounds from Egyptian sea stars against seasonal influenza A/H1N1 virus J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Nadia I. Okasha, Mohamed Abdel Rahman, Mohammed S. Nafie, Noura M. Abo Shama, Ahmed Mostafa, Dalia A. El-Ebeedy, Ahmed Z. Abdel Azeiz
One of the most dangerous problems that the world faced recently is viral respiratory pathogens. Marine creatures, including specially class (starfish) have been extensively studied due to their miscellaneous bioactivities, excellent pharmacological properties, and complex secondary metabolites, including steroids, steroidal glycosides, anthraquinones, alkaloids, phospholipids, peptides, and fatty
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Ameliorating orthodontic relapse using laser bio-stimulation and mesenchymal stem cells in rats J. Genet. Eng. Biotechnol. Pub Date : 2024-01-22 Samer S. Othman, Ali Saafan, Mohammad M.F. Al-Halbosiy, Iman Fathy, Mohamed Khursheed Alam, Amr R. El-Beialy, Hanady S. Al-Shmgani, Ghassan M. Sulaiman
Orthodontic relapse is a frequent problem that many patients experience. Although orthodontic therapy has advanced, recurrence rates can still reach 90%. We undertook a study to look at the possibilities of laser bio-stimulation and stem cells because they have showed promising outcomes in lowering recurrence rates. Our objective was to analyze the effects of Low-level laser therapy (LLLT) and Mesenchymal
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Physiochemical analyses and molecular characterization of heavy metal-resistant bacteria from Ilesha gold mining sites in Nigeria J. Genet. Eng. Biotechnol. Pub Date : 2023-12-22 Glory Jesutomisin Ojo, Olugbenga Samson Onile, Abdul Onoruoiza Momoh, Bolaji Fatai Oyeyemi, Victor Omoboyede, Adeyinka Ignatius Fadahunsi, Tolulope Onile
The contribution of the processes involved and waste generated during gold mining to the increment of heavy metals concentration in the environment has been well established. While certain heavy metals are required for the normal functioning of an organism, certain heavy metals have been identified for their deleterious effects on the ecosystem and non-physiological roles in organisms. Hence, efforts
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Acetaminophen-traces bioremediation with novel phenotypically and genotypically characterized 2 Streptomyces strains using chemo-informatics, in vivo, and in vitro experiments for cytotoxicity and biological activity J. Genet. Eng. Biotechnol. Pub Date : 2023-12-19 Donia H. Embarez, Ahmed S. Abdel Razek, Emad B. Basalious, Magdi Mahmoud, Nadia M. Hamdy
We isolated two novel bacterial strains, active against the environmental pollutant acetaminophen/Paracetamol®. Streptomyces chrestomyceticus (symbol RS2) and Flavofuscus (symbol M33) collected from El-Natrun Valley, Egypt—water, sediment, and sand samples, taxonomically characterized using a transmission electron microscope (TEM). Genotypic identification, based on 16S rRNA gene sequence analysis
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Biosoftening of banana pseudostem fiber using cellulase and pectinase enzyme isolated from Aspergillus niger for textile industry J. Genet. Eng. Biotechnol. Pub Date : 2023-12-18 Manjusha W. A., Josphine J. S., Deepa P. K., Sujatha S., Ahil Raj S.
Nowadays, farmers are facing a lot of problems for the disposal of banana pseudostem waste after the harvesting of banana. Banana pseudostem is a rich source of fiber, which is an alternative source of other natural and synthetic fibers. The banana fibers are biodegradable, and they are expected to be in great demand in the international market. For the textile industry, fibers were extracted using
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FolE gene expression for folic acid productivity from optimized and characterized probiotic Lactobacillus delbrueckii J. Genet. Eng. Biotechnol. Pub Date : 2023-12-18 Mohamed Khedr, Fady Sayed Youssef, Noura El-kattan, Mahmoud S. Abozahra, Mohammed N. Selim, Abdullah Yousef, Kamal M. A. Khalil, Alsayed E. Mekky
Lactobacillus delbrueckii was one of the most common milk lactic acid bacterial strains (LAB) which characterized as probiotic with many health influencing properties. Among seven isolates, KH1 isolate was the best producer of folic acid with 100 µg/ml after 48 h of incubation; FolE gene expression after 24 h of incubation was in the highest value in case of KH1 with three folds. Lactose was the best
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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primer design based on Indonesia SARS-CoV-2 RNA sequence J. Genet. Eng. Biotechnol. Pub Date : 2023-12-18 Irsyad Ibadurrahman, Suryani, Desriani
The COVID-19 pandemic has highlighted the importance of tracking cases by using various methods such as the Reverse transcription loop-mediated isothermal amplification (RT-LAMP) which is a fast, simple, inexpensive, and accurate mass tracker. However, there have been no reports about the development of RT-LAMP primer designs that use genome sequences of viruses from Indonesia. Therefore, this study
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In silico analysis of HLA-1 and HLA-2 recognition of a designed recombinant human papillomavirus vaccine based on L1 protein HPV subtype 45 J. Genet. Eng. Biotechnol. Pub Date : 2023-12-13 Asri Sulfianti, Nihayatul Karimah, Astutiati Nurhasanah
Human leukocyte antigen (HLA) can bind and present the processed antigenic peptide derived from the vaccine to the T cell receptor, and this capability is crucial in determining the effectivity of the vaccine to terminate virus-infected cells, activate macrophages, and induce B cells to produce antibodies. A recombinant vaccine candidate based on protein L1 HPV45 was designed and analysed whether it
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In silico design of an epitope-based vaccine against PspC in Streptococcus pneumoniae using reverse vaccinology J. Genet. Eng. Biotechnol. Pub Date : 2023-12-12 Md. Nahian, Muhammad Shahab, Lincon Mazumder, Jonas Ivan Nobre Oliveira, Tanjina Akhtar Banu, Murshed Hasan Sarkar, Barna Goswami, Ahashan Habib, Shamima Begum, Shahina Akter
Streptococcus pneumoniae is a major pathogen that poses a significant hazard to global health, causing a variety of infections including pneumonia, meningitis, and sepsis. The emergence of antibiotic-resistant strains has increased the difficulty of conventional antibiotic treatment, highlighting the need for alternative therapies such as multi-epitope vaccines. In this study, immunoinformatics algorithms
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A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling J. Genet. Eng. Biotechnol. Pub Date : 2023-12-12 Nadia A. Soliman, Safaa M. Ali, Mahmoud E. A. Duab, Yasser R. Abdel-Fattah
As a white biotechnological trend, esterases are thought to be among the most active enzymes’ classes in biocatalysis and synthesis of industrially importance organic compounds. Esterases are used in many applications such as the manufacture of pharmaceuticals, cosmetics, leather, textile, paper, food, dairy products, detergents, and treatment of some environmental pollutants. A poly-histidine moiety
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Correction: Mycosynthesis of silver nanoparticles using marine fungi and their antimicrobial activity against pathogenic microorganisms J. Genet. Eng. Biotechnol. Pub Date : 2023-12-08 Manar A. Basheer, Khaled Abutaleb, Nermine N. Abed, Amal A. I. Mekawey
Correction: J Genet Eng Biotechnol 21, 127 (2023) https://doi.org/10.1186/s43141-023-00572-z In the original version of this article [1], affiliations 3 and 4 were transposed due to a typesetting error. The affiliations appear correctly in this erratum, and the original article has been corrected. Basheer MA, Abutaleb K, Abed NN et al (2023) Mycosynthesis of silver nanoparticles using marine fungi
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Whole genome sequence and comparative genomics analysis of multidrug-resistant Staphylococcus xylosus NM36 isolated from a cow with mastitis in Basrah city J. Genet. Eng. Biotechnol. Pub Date : 2023-12-07 Hassan M. Al-Tameemi, Husam Al-Hraishawi, Murtakab Y. Al-Hejjaj, Noor S. Abdulah, Haider R. Alrafas, Yessar A. Dawood
Staphylococcus xylosus is a coagulase-negative, gram-positive coccus that is found in the environment and as a commensal organism on the skin and mucosal surfaces of animals. Despite the fact that S. xylosus is considered a nonpathogenic bacterium, several studies have linked S. xylosus to opportunistic infections in both animals and humans. During an investigation of mastitis-causing agents in the
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Immunoinformatics-aided rational design of multiepitope-based peptide vaccine (MEBV) targeting human parainfluenza virus 3 (HPIV-3) stable proteins J. Genet. Eng. Biotechnol. Pub Date : 2023-12-06 Md Sakib Hossen, Md. Nazmul Hasan, Munima Haque, Tawsif Al Arian, Sajal Kumar Halder, Md. Jasim Uddin, M. Abdullah-Al-Mamun, Md Salman Shakil
Human parainfluenza viruses (HPIVs) are common RNA viruses responsible for respiratory tract infections. Human parainfluenza virus 3 (HPIV-3) is particularly pathogenic, causing severe illnesses with no effective vaccine or therapy available. The current study employed a systematic immunoinformatic/reverse vaccinology approach to design a multiple epitope-based peptide vaccine against HPIV-3 by analyzing
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Isolation of plant growth-promoting rhizobacteria from the agricultural fields of Tattiannaram, Telangana J. Genet. Eng. Biotechnol. Pub Date : 2023-12-06 Gottumukkala Hiranmayee, Debankona Marik, Ayan Sadhukhan, Golamari Siva Reddy
Plant probiotics bacteria are live microbes that promote soil health and plant growth and build the stress-tolerant capacity to the plants. They benefit the plants by increasing nutrient absorption and release of stress-related phytohormones. These plant probiotic bacteria serve a better purpose to the plant when compared to chemical fertilizers. Use of chemical fertilizers such as arsenic and cadmium
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Exploring structural antigens of yellow fever virus to design multi-epitope subunit vaccine candidate by utilizing an immuno-informatics approach J. Genet. Eng. Biotechnol. Pub Date : 2023-12-05 Kiran Sura, Himanshi Rohilla, Dev Kumar, Ritu Jakhar, Vaishali Ahlawat, Deepshikha Kaushik, Mehak Dangi, Anil Kumar Chhillar
Yellow fever is a mosquito-borne viral hemorrhagic disease transmitted by several species of virus-infected mosquitoes endemic to tropical regions of Central and South America and Africa. Earlier in the twentieth century, mass vaccination integrated with mosquito control was implemented to eradicate the yellow fever virus. However, regular outbreaks occur in these regions which pose a threat to travelers
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Short tandem repeat (STR) variation from 6 cities in Iraq based on 15 loci J. Genet. Eng. Biotechnol. Pub Date : 2023-12-05 Majeed A. Sabbah, Mohammed M. Al-Zubaidi, Thooalnoon Y. Al-janabi, Dhuha S. Namaa, Haider K. Al-rubai, Hala K. Ibrahem
One thousand sixty-one individuals were sampled from the cities of Anbar, Baghdad, Basra, Diyala, Najaf, and Wasit in Iraq and typed for 15 forensic STRs to explore the genetic structure of Iraq and develop a forensic DNA database. The total number of alleles that were identified was 203. Analyses of molecular variance (AMOVA) were then conducted Baghdad provides a good representation of the rest of
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The hepato- and neuroprotective effect of gold Casuarina equisetifolia bark nano-extract against Chlorpyrifos-induced toxicity in rats J. Genet. Eng. Biotechnol. Pub Date : 2023-12-01 Wael Mahmoud Aboulthana, Noha El-Sayed Ibrahim, Amgad Kamal Hassan, Wagdy Khalil Bassaly, Hassan Abdel-Gawad, Hamdy Ahmed Taha, Kawkab A. Ahmed
The bark of Casuarina equisetifolia contains several active phytoconstituents that are suitable for the biosynthesis of gold nanoparticles (Au-NPs). These nanoparticles were subsequently evaluated for their effectiveness in reducing the toxicity induced by Chlorpyrifos (CPF) in rats. Various hematological and biochemical measurements were conducted in this study. In addition, markers of oxidative stress
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Cloning and characterization of an acidic lipase from a lipolytic bacterium in tempeh J. Genet. Eng. Biotechnol. Pub Date : 2023-12-01 Naswandi Nur, Antonius Suwanto, Anja Meryandini, Maggy Thenawidjaja Suhartono, Esti Puspitasari, Hyung Kwoun Kim
Lipases have emerged as essential biocatalysts, having the ability to contribute to a wide range of industrial applications. Microbial lipases have garnered significant industrial attention due to their stability, selectivity, and broad substrate specificity. In the previous study, a unique lipolytic bacterium (Micrococcus luteus EMP48-D) was isolated from tempeh. It turns out the bacteria produce
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Recent advances in genome annotation and synthetic biology for the development of microbial chassis J. Genet. Eng. Biotechnol. Pub Date : 2023-12-01 Saltiel Hamese, Kanganwiro Mugwanda, Mutsa Takundwa, Earl Prinsloo, Deepak B. Thimiri Govinda Raj
This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on
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In-silico analysis of potent Mosquirix vaccine adjuvant leads J. Genet. Eng. Biotechnol. Pub Date : 2023-11-30 Okello Harrison Onyango, Cynthia Mugo Mwenda, Grace Gitau, John Muoma, Patrick Okoth
World Health Organization recommend the use of malaria vaccine, Mosquirix, as a malaria prevention strategy. However, Mosquirix has failed to reduce the global burden of malaria because of its inefficacy. The Mosquirix vaccine’s modest effectiveness against malaria, 36% among kids aged 5 to 17 months who need at least four doses, fails to aid malaria eradication. Therefore, highly effective and efficacious
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Influenza vaccine: a review on current scenario and future prospects J. Genet. Eng. Biotechnol. Pub Date : 2023-11-30 Dipanshi Gupta, Sumedha Mohan
Vaccination is a crucial tool in preventing influenza, but it requires annual updates in vaccine composition due to the ever-changing nature of the flu virus. While healthcare and economic burdens have reduced, the virus remains a challenge. Research conducted over the past decade has revealed pathways for improvement through both basic and clinical studies. Viral surveillance plays a vital role in
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Endophytic bacteria Klebsiella spp. and Bacillus spp. from Alternanthera philoxeroides in Madiwala Lake exhibit additive plant growth-promoting and biocontrol activities J. Genet. Eng. Biotechnol. Pub Date : 2023-11-30 Soma Biswas, Indhu Philip, Saranya Jayaram, Suma Sarojini
The worldwide increase in human population and environmental damage has put immense pressure on the overall global crop production making it inadequate to feed the entire population. Therefore, the need for sustainable and environment-friendly practices to enhance agricultural productivity is a pressing priority. Endophytic bacteria with plant growth-promoting ability and biocontrol activity can strongly
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Immunoinformatics analysis of Brucella melitensis to approach a suitable vaccine against brucellosis J. Genet. Eng. Biotechnol. Pub Date : 2023-11-29 Pejman Hashemzadeh, Saba Asgari nezhad, Hossein Khoshkhabar
Brucellosis caused by B. melitensis is one of the most important common diseases between humans and livestock. Currently, live attenuated vaccines are used for this disease, which causes many problems, and unfortunately, there is no effective vaccine for human brucellosis. The aim of our research was to design a recombinant vaccine containing potential immunogenic epitopes against B. melitensis. In
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Enhancement effect of AgO nanoparticles on fermentative cellulase activity from thermophilic Bacillus subtilis Ag-PQ J. Genet. Eng. Biotechnol. Pub Date : 2023-11-29 Saddam Hussain, Muhammad Talha Yasin, Khurshid Ahmad, Suleman Khan, Rasheed Ahmad, Jallat Khan, Abdul Ghani, Muhammad Musaddiq Shah, Muzzamil Ahmed, Hasnat Tariq, Hamid Rehman, Adil Hussain, Muhammad Faheem, Syed Ali Imran Bokhari
Cellulase is an important bioprocessing enzyme used in various industries. This study was conducted with the aim of improving the biodegradation activity of cellulase obtained from the Bacillus subtilis AG-PQ strain. For this purpose, AgO and FeO NPs were fabricated using AgNO3 and FeSO4·7H2O salt respectively through a hydro-thermal method based on five major steps; selection of research-grade materials
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Studying the pathogenicity of 26 variants characterized in the first molecular analyses of Egyptian aplastic anemia patients J. Genet. Eng. Biotechnol. Pub Date : 2023-11-29 Mona F. Sokkar, Mona Hamdy, Peter SF Erian, Rehab M. Mosaad, Nesma M. Elaraby, Mohamed B. Taher, Heba El-Sayed, Mohammed Al Komy, Maha M. Eid, Amal M. Mohamed, Khalda S. Amr, Ghada Y. El-Kamah
Aplastic anemia (AA) is a bone marrow disorder characterized by peripheral pancytopenia and marrow hypoplasia which can lead to life-threatening complications. Our objective was to study the telomerase genes (TERT and TERC) variants, explore their relationship to telomere shortening and TERT gene expression, and to identify variants in the MPL gene within Egyptian AA patients. Forty AA patients and
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Optimizing the generation of mature bone marrow-derived dendritic cells in vitro: a factorial study design J. Genet. Eng. Biotechnol. Pub Date : 2023-11-29 Najla Alotaibi, Alia Aldahlawi, Kawther Zaher, Fatemah Basingab, Jehan Alrahimi
Factorial design is a simple, yet elegant method to investigate the effect of multiple factors and their interaction on a specific response simultaneously. Hence, this type of study design reaches the best optimization conditions of a process. Although the interaction between the variables is widely prevalent in cell culture procedures, factorial design per se is infrequently utilized in improving
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Biodiversity and biological applications of marine actinomycetes—Abu-Qir Bay, Mediterranean Sea, Egypt J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Ghada E. Hegazy, Zakia A. Olama, G. M. Abou-Elela, Heba S. Ramadan, Walaa M. Ibrahim, Dalia El S. El Badan
The ability of actinomycetes to produce bioactive secondary metabolites makes them one of the most important prokaryotes. Marine actinomycetes are one of the most important secondary metabolites producers used for pharmaceuticals and other different industries. In this study, the promising actinomycetes were isolated from Abu-Qir Bay. Four different media named as starch nitrate, starch casein, glycerol
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A computational simulation appraisal of banana lectin as a potential anti-SARS-CoV-2 candidate by targeting the receptor-binding domain J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Sofia Safitri Hessel, Fenny Martha Dwivany, Ima Mulyama Zainuddin, Ketut Wikantika, Ismail Celik, Talha Bin Emran, Trina Ekawati Tallei
The ongoing concern surrounding coronavirus disease 2019 (COVID-19) primarily stems from continuous mutations in the genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), leading to the emergence of numerous variants. The receptor-binding domain (RBD) in the S1 subunit of the S protein of the virus plays a crucial role in recognizing the host’s angiotensin-converting enzyme 2
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Metagenomic analysis reveals diverse microbial community and potential functional roles in Baner rivulet, India J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Bhavna Brar, Ravi Kumar, Dixit Sharma, Amit Kumar Sharma, Kushal Thakur, Danish Mahajan, Rakesh Kumar
The health index of any population is directly correlated with the water quality, which in turn depends upon physicochemical characteristics and the microbiome of that aquatic source. For maintaining the water quality, knowledge of microbial diversity is a must. The present investigation attempts to evaluate the microflora of Baner. Metagenomics has been proven to be the technique for examining the
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Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Harish Babu Kolla, Shivakiran Satyanarayan Makam, Prakash Narayana Reddy
Members of Enterobacteriaceae such as Escherichia coli O 157:H7, Salmonella sp., Shigella sp., Klebsiella sp., and Citrobacter freundii are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of Enterobacteriaceae are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly
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Dual action of epigallocatechin-3-gallate in virus-induced cell Injury J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Ahmed Mostafa, Gomaa Mostafa-Hedeab, Hany Abdelfattah Elhady, Esraa Ahmed Mohamed, Abozer Y. Eledrdery, Sager Holyl Alruwaili, Ahmed Mohamed Al-Abd, Abdou Kamal Allayeh
Viral infections cause damage and long-term injury to infected human tissues, demanding therapy with antiviral and wound healing medications. Consequently, safe phytochemical molecules that may control viral infections with an ability to provide wound healing to viral-induced tissue injuries, either topically or systemically, are advantageous. Herein, we hypothesized that epigallocatechin-3-gallate
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Designing a novel and combinatorial multi-antigenic epitope-based vaccine “MarVax” against Marburg virus—a reverse vaccinology and immunoinformatics approach J. Genet. Eng. Biotechnol. Pub Date : 2023-11-28 Bishal Debroy, Sribas Chowdhury, Kuntal Pal
Marburg virus (MARV) is a member of the Filoviridae family and causes Marburg virus disease (MVD) among humans and primates. With fatality rates going up to 88%, there is currently no commercialized cure or vaccine to combat the infection. The National Institute of Allergy and Infectious Diseases (NIAID) classified MARV as priority pathogen A, which presages the need for a vaccine candidate which can
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Bioinformatics study of phytase from Aspergillus niger for use as feed additive in livestock feed J. Genet. Eng. Biotechnol. Pub Date : 2023-11-27 Hamdan Maulana, Yantyati Widyastuti, Nina Herlina, Abun Hasbuna, Aas Syiarudin Hasbi Al-Islahi, Lita Triratna, Novi Mayasari
Phytase supplementation in rations can reduce their phytic acid composition in order to enhance their nutritional value. Aspergillus niger is a fungus that can encode phytase. This study aims to determine the characteristics of its DNA sequences and amino acid composition that encode the phytase enzyme, as well as to determine the primer designs. This study used gene sequence data and protein-encoding