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Catalytic flash pyrolysis of Monoraphidium sp. before and after lipid extraction Algal. Res. (IF 4.008) Pub Date : 2021-01-21 Thalita M. Delmiro; Rebecca R.Y.O.V. Wilson; Dulce M.A. Melo; Graco A.C.M. Viana; Leonardo B.B. Mendes; Renata M. Braga
The use of biomass from Monoraphidium sp. microalgae before and after lipid extraction was investigated using flash catalytic pyrolysis to generate a bio-oil with improved characteristics. The biomass was obtained from intensive outdoor crops and exhibited an attractive biochemical composition to be used in thermochemical processes with high protein and carbohydrate content remaining after lipid extraction
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Driving toward sustainable algal fuels: A harmonization of techno-economic and life cycle assessments Algal. Res. (IF 4.008) Pub Date : 2021-01-21 Jesse R. Cruce; Audrey Beattie; Peter Chen; David Quiroz; Michael Somers; Samuel Compton; Katherine DeRose; Braden Beckstrom; Jason C. Quinn
Research efforts have investigated a range of system designs for algae growth and conversion into biofuels. The economic feasibility and environmental impact of these systems are frequently evaluated through techno-economic analyses (TEA) and life-cycle assessment (LCA), which typically determine the levelized cost of fuel production in gallons of gasoline equivalent (MFSP, in $-gge−1), global warming
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Mixotrophic cultivation of Thalassiosira pseudonana with pure and crude glycerol: Impact on lipid profile Algal. Res. (IF 4.008) Pub Date : 2021-01-20 Costanza Baldisserotto; Alessandra Sabia; Alessandra Guerrini; Sara Demaria; Michele Maglie; Lorenzo Ferroni; Simonetta Pancaldi
The mixotrophic cultivation of microalgae often couples high biomass production with lipid accumulation, and thus it is considered a useful approach to obtain high quantities of added value microalgal biomass. In this study, the marine diatom Thalassiosira pseudonana was supplied with glycerol to determine the potential of the mixotrophic alga for the accumulation of lipids. In preliminarily experiments
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Osmotic shock pre-treatment of Chaetoceros muelleri wet biomass enhanced solvent-free lipid extraction and biogas production Algal. Res. (IF 4.008) Pub Date : 2021-01-20 Lina María González-González; Sergi Astals; Steven Pratt; Paul D. Jensen; Peer M. Schenk
An integrated system for biodiesel and biogas production for the diatom Chaetoceros muelleri was evaluated. We assessed the suitability of osmotic shock pre-treatment of wet microalgal biomass for lipid extraction and biogas production. Osmotic shock pre-treatment released up to 72% of C. muelleri lipid content. Biogas production of pre-treated and lipid-spent microalgae was evaluated by biomethane
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Biological and chemical characterization of new isolated halophilic microorganisms from saltern ponds of Trapani, Sicily Algal. Res. (IF 4.008) Pub Date : 2021-01-19 Valeria Villanova; Christian Galasso; Federica Fiorini; Serena Lima; Mark Brönstrup; Clementina Sansone; Christophe Brunet; Alberto Brucato; Francesca Scargiali
Halophilic microorganisms inhabiting hypersaline environments such as salt lakes, Dead Sea, or salt evaporation ponds, have acquired specific cell adaptation to grow within stressful conditions. In this study, we isolated heterotrophic and autotrophic microorganisms from several saltern ponds located at the Natural Reserve “Saline di Trapani e Paceco”, Sicily, Italy. The aim of the study was to investigate
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Evaluation of Galdieria sulphuraria for nitrogen removal and biomass production from raw landfill leachate Algal. Res. (IF 4.008) Pub Date : 2021-01-16 Shanglei Pan; Kyleigh L. Dixon; Tabish Nawaz; Ashiqur Rahman; Thinesh Selvaratnam
This study evaluates the potential of thermophilic mixotrophic alga, Galdieria sulphuraria, to efficiently perform on-site treatment of landfill leachate (LL). Nutrient removal and algae growth experiments using various dilutions (20%, 40%, 60%, 80%, 100%) of actual municipal LL were carried out in a 14-day lab scale set-up. The effect of N/P mass ratio (255:1; 100:1; 50:1; 25:1; 10:1; 4.55:1) was
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Choreography of multiple omics reveals the mechanism of lipid turnover in Schizochytrium sp. S31 Algal. Res. (IF 4.008) Pub Date : 2021-01-16 Ming Chang; Tao Zhang; Leilei Li; Fei Lou; Meimei Ma; Ruijie Liu; Qingzhe Jin; Xingguo Wang
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Microalgal-bacterial granular sludge for municipal wastewater treatment under simulated natural diel cycles: Performances-metabolic pathways-microbial community nexus Algal. Res. (IF 4.008) Pub Date : 2021-01-14 Bin Ji; Shulian Wang; Mahamalage Rochelle Udani Silva; Meng Zhang; Yu Liu
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Role of melatonin in regulation of lipid accumulation, autophagy and salinity-induced oxidative stress in microalga Monoraphidium sp. QLY-1 Algal. Res. (IF 4.008) Pub Date : 2021-01-14 Yongteng Zhao; Xueting Song; Peng Zhao; Tao Li; Jun-Wei Xu; Xuya Yu
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The effects of illumination and trophic strategy on gene expression in Chlamydomonas reinhardtii Algal. Res. (IF 4.008) Pub Date : 2021-01-14 Victor Sanchez-Tarre; Alexandros Kiparissides
Microalgae are of substantial biotechnological interest due their polyphyletic nature which grants them access to a wide array of high-value metabolites. The inherent genetic diversity of microalgae combined with their metabolic plasticity when grown using different trophic and illumination strategies necessitate the establishment of a reference knowledge base. In the present study we present a detailed
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Astaxanthin as a microalgal metabolite for aquaculture: A review on the synthetic mechanisms, production techniques, and practical application Algal. Res. (IF 4.008) Pub Date : 2021-01-13 Qian Lu; Huankai Li; Yao Zou; Hui Liu; Limin Yang
Astaxanthin, which is an important metabolite with anti-oxidative property, has been discovered in some fish, shrimp, and microorganisms. Owing to the great advantages of microalgae-based astaxanthin in the aspects of sustainability and safety, microalgal astaxanthin is emerging into the limelight. In the past years, researchers isolated a couple of microalgal species and explored the synthesis pathways
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Sargassum blooms in the Atlantic Ocean – From a burden to an asset Algal. Res. (IF 4.008) Pub Date : 2021-01-14 Ute C. Marx; John Roles; Ben Hankamer
Recently there has been a significant increase in the amount and frequency of seaweed blooms of the holopelagic brown macroalgae Sargassum fluitans and natans (hereafter Sargassum) in the Atlantic Ocean. These blooms impose a major burden on residents (e.g. rotting beached Sargassum, unpleasant odor, toxic gases), the local economy of countries with affected coast lines (e.g. impacting tourism and
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A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae Algal. Res. (IF 4.008) Pub Date : 2021-01-11 Ayat J.S. Al-Azab; Dwiyantari Widyaningrum; Haruna Hirakawa; Yashuko Hayashi; Satoshi Tanaka; Takeshi Ohama
We examined the potency of resin nanoparticles (NPs) to induce cell mortality in diverse non-green algal species (e.g., species in SAR and Hacrobia clades) and the difference in sensitivity to NPs among the species. In total, 18 non-green algal species were co-incubated with laboratory-prepared NPs (mean diameter, 180 nm) composed of poly (isobutylcyanoacrylate) resin NPs (iBCA-NPs). Exposure to 100 mg L−1
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Biomolecule composition and draft genome of a novel, high-lipid producing Scenedesmaceae microalga Algal. Res. (IF 4.008) Pub Date : 2021-01-09 Vilmos Soós; Prateek Shetty; Gergely Maróti; Norbert Incze; Eszter Badics; Péter Bálint; Vince Ördög; Ervin Balázs
Lipid biosynthesis in microalgae can be stimulated by cultivation in low nitrogen medium. MACC-401 was isolated from the soil surface in Tres Marias (MG-Brazil). The strain shows the morphological characteristics of the Scenedesmaceae green algae. The daily biomass and lipid production of MACC-401 is remarkable, 0.36 g L−1 and 110 mg L−1, respectively. Exploration of the genetic background of this
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Transcriptome dynamics and hub genes of green alga Nannochloris sp. JB17 under NaHCO3 stress Algal. Res. (IF 4.008) Pub Date : 2021-01-09 Min Wang; Xiaoxue Ye; Yao Wang; Dan Su; Shenkui Liu; Yuanyuan Bu
Soil salinization and alkalization are major problems in crop production. To gain insights into the mechanisms of saline-alkaline tolerance, we investigated the molecular response of a highly stress tolerant green alga, Nannochloris sp. JB17 (JB17) to NaHCO3. Results indicated that JB17 cells retain a high level of cell viability when subjected to a high concentration (300 mM) of NaHCO3. A transcriptome
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Enhancing resistance of Chlamydomonas reinhardtii to oxidative stress fusing constructs of heterologous antioxidant peptides into D1 protein Algal. Res. (IF 4.008) Pub Date : 2021-01-09 Amina Antonacci; Ivo Bertalan; Maria Teresa Giardi; Viviana Scognamiglio; Mehmet Turemis; Dirk Fisher; Udo Johanningmeier
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Photosynthetic performance of Chlorella vulgaris R117 mass culture is moderated by diurnal oxygen gradients in an outdoor thin layer cascade Algal. Res. (IF 4.008) Pub Date : 2021-01-10 Tomás Agustín Rearte; Paula S.M. Celis-Plá; Amir Neori; Jiří Masojídek; Giuseppe Torzillo; Cintia Gómez-Serrano; Ana Margarita Silva Benavides; Félix Álvarez-Gómez; R.T. Abdala-Díaz; Karolína Ranglová; Martín Caporgno; Thaís Fávero Massocato; Jaqueline Carmo da Silva; Hafidh Al Mahrouqui; Richard Atzmüller; Félix L. Figueroa
Dissolved oxygen concentration is a critical point for microalgae in large scale cultivation systems. Highly productive cultures inevitably generate a build-up of oxygen gradients along the reactor which can affect photosynthetic performance. In this study, a fast-growing strain of Chlorella vulgaris R117 was cultured outdoors in a thin-layer cascade during a one-week trial reaching a biomass productivity
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Harvesting microalgae for health beneficial dietary supplements Algal. Res. (IF 4.008) Pub Date : 2021-01-10 C.A. Laamanen; S.M. Desjardins; G.N.A. Senhorinho; J.A. Scott
Microalgae are an abundant and diverse resource that can help ease the growing pressure on the food and dietary supplements industry. Microalgae can be used either as whole biomass to increase the overall food content of lipids, carbohydrates, and proteins. They can be also grown to produce high levels of specific metabolites that are extracted and purified, such as omega-3 fatty acids, antioxidants
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Cultivation of heterotrophic algae on paper waste material and digestate Algal. Res. (IF 4.008) Pub Date : 2021-01-09 Oskar Haske-Cornelius; Sonja Gierlinger; Robert Vielnascher; Wolfgang Gabauer; Katharina Prall; Alessandro Pellis; Georg M. Guebitz
This study investigates the cellulases-catalyzed hydrolysis of newspaper waste and paper slurries as carbon source in combination with digestate from anaerobic digestion as nitrogen source for the heterotrophic cultivation of Chlorella sorokiniana. The enzymatic hydrolysis of paper slurries resulted in a hydrolysate containing 27.2 g l−1 glucose reflecting a 78% cellulose conversion. A maximum Chlorella
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Acclimation potential and biochemical response of four temperate macroalgae to light and future seasonal temperature scenarios Algal. Res. (IF 4.008) Pub Date : 2021-01-07 Matthias Schmid; Freddy Guihéneuf; Udo Nitschke; Dagmar B. Stengel
Resilience and biochemical response to predicted future North Atlantic Ocean temperature conditions (+2 °C of current seasonal observations) and different light conditions were investigated in four commercially valuable macroalgae that naturally inhabit different shore levels (Ascophyllum nodosum, Fucus serratus, Laminaria digitata and Palmaria palmata). Increased irradiances had a positive effect
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Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis Algal. Res. (IF 4.008) Pub Date : 2021-01-05 Xiaodong Wang; Xuexia Miao; Gao Chen; Yulin Cui; Fengjie Sun; Jianhua Fan; Zhengquan Gao; Chunxiao Meng
The growth and astaxanthin production of unicellular Haematococcus pluvialis is affected by both the high light and sodium acetate (NaAC) stresses. There are sparse on studies of the regulatory pathways of microRNAs (miRNAs) and their target genes in response to high light and NaAC stresses in H. pluvialis. In this study, transcriptomic analysis was used to identify the key target genes of miRNA regulation
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Photoautotrophic production of p-Coumaric acid using genetically engineered Synechocystis sp. Pasteur Culture Collection 6803 Algal. Res. (IF 4.008) Pub Date : 2021-01-06 E-Bin Gao; Kwasi Kyere-Yeboah; Junhua Wu; Haiyan Qiu
In view of the environmental problems associated with climate change and greenhouse gas emissions, the potential of carbon sequestration has emerged as a research hotspot. Cyanobacteria can use solar energy and carbon dioxide to produce organic molecules bypassing the need for carbohydrate feedstock. This valuable trait has spearheaded its use in the field of bioproduction and ultimately metabolic
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Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa: Subcellular localization and role in triacylglycerol synthesis Algal. Res. (IF 4.008) Pub Date : 2021-01-06 Li-Ping Sun; Long-Ling Ouyang; Hong Bao; Jian-Guo Liu; Zheng Sun; Zhi-Gang Zhou
Microalgae represent a rich and naturally occurring source of triacylglycerol (TAG), and the microalgae-derived TAG has enormous potential in bioenergy, bio-based materials and other agricultural innovations. Glycerol-3-phosphate acyltransferase (GPAT) is a key enzyme that catalyzes the biosynthesis of TAG. Previously our lab has reported the first GPAT in the oleaginous alga Myrmecia incisa, and in
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Consumer knowledge and attitudes towards microalgae as food: The case of Spain Algal. Res. (IF 4.008) Pub Date : 2021-01-05 Tomás Lafarga; Ruth Rodríguez-Bermúdez; Ainoa Morillas-España; Silvia Villaró; Marco García-Vaquero; Lara Morán; Ana Sánchez-Zurano; Cynthia Victoria González-López; Francisco Gabriel Acién-Fernández
Spanish consumers' knowledge and attitudes towards microalgae as food were assessed using a specific questionnaire - a total of 3084 Spanish consumers over 18 years old were considered in the current study. Overall, the questionnaire revealed that there is a huge lack of knowledge on basic aspects of microalgae, especially within the youngest respondents (18–24 years old) and for those aged over 65 years
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Simultaneous improvement of astaxanthin and lipid production of Haematococcus pluvialis by using walnut shell extracts Algal. Res. (IF 4.008) Pub Date : 2020-12-29 Chunli Yu; Xingyu Li; Benyong Han; Yongteng Zhao; Shuxiang Geng; Delu Ning; Ting Ma; Xuya Yu
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Are we out of the infancy of microalgae-based drug discovery? Algal. Res. (IF 4.008) Pub Date : 2020-12-26 Saverio Savio; Roberta Congestri; Carlo Rodolfo
In the last 20 years, we have witnessed increased interest in prospecting novel drugs from microalgae-derived bioactive metabolites. However, despite the conspicuous amount of literature about bioactivity screening of microalgal extracts, we have found few investigations focusing on the subsequent phases of the drug discovery process. We identify here the main critical aspects of the reported approaches
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The impact of seasonal variations about temperature and photoperiod on the treatment of municipal wastewater by algae-bacteria system in lab-scale Algal. Res. (IF 4.008) Pub Date : 2020-12-26 Kaiwei Xu; Xiaotong Zou; Yating Xue; Yanhui Qu; Yanpeng Li
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Production of hydroxy fatty acids and its effects on photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803 Algal. Res. (IF 4.008) Pub Date : 2020-12-24 Takashi Inada; Shuntaro Machida; Koichiro Awai; Iwane Suzuki
Microalgal lipids and fatty acids are important components for achieving biofuel because of their potential high productivity. Although fatty acids that have a hydroxy group adjacent to the end of the acyl chain might be an important chemical feedstock, most algae do not accumulate it. To produce (ω−1)-hydroxy fatty acids from 3-hydroxybutylyl-CoA, an intermediate for polyhydroxybutylate biosynthesis
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Effects of seeding method, timing and site selection on the production and quality of sugar kelp, Saccharina latissima: A Danish case study Algal. Res. (IF 4.008) Pub Date : 2020-12-24 Teis Boderskov; Mette Møller Nielsen; Michael Bo Rasmussen; Thorsten Johannes Skovbjerg Balsby; Adrian Macleod; Susan Løvstad Holdt; Jens Jørgen Sloth; Annette Bruhn
In recent years, research projects and enterprises have documented that the sugar kelp, Saccharina latissima, can be successfully cultivated in Northern European waters. There is a need however, for optimizing production methods to achieve an economically viable and competitive business. A novel direct seeding method, applying juvenile sporophytes directly onto textiles immediately before deployment
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Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum Algal. Res. (IF 4.008) Pub Date : 2020-12-23 Matthias Windhagauer; Raffaela M. Abbriano; Justin Ashworth; Lorenzo Barolo; Ana Cristina Jaramillo-Madrid; Mathieu Pernice; Martina A. Doblin
Phaeodactylum tricornutum is a model pennate diatom for molecular studies due to its sequenced genome, genetic tractability, and capacity to produce a myriad of compounds of biotechnological interest. While tools for genetic engineering and heterologous gene expression have been developed for this species, the available set of characterised promoters remains limited. A diverse and well-understood set
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Comparative transcriptome analysis of short-term responses to salt and glycerol hyperosmotic stress in the green alga Dunaliella salina Algal. Res. (IF 4.008) Pub Date : 2020-12-22 Hexin Lv; Minjae Kim; Seunghye Park; Kwangryul Baek; Hyeonjun Oh; Juergen E.W. Polle; EonSeon Jin
Dunaliella salina is a model photosynthetic organism for studying osmoregulation. To further understand the molecular basis of short-term hyperosmotic-stress responses, gene expression was investigated by RNA-Seq of D. salina cells sampled at 15, 30, 60, and 120 min following exposure to salt and glycerol stress. De novo reconstruction of the transcriptome using Illumina paired-end reads generated
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Activation of MAPK signaling in response to nitrogen deficiency in Ulva prolifera (Chlorophyta) Algal. Res. (IF 4.008) Pub Date : 2020-12-18 Juanjuan Yang; Yi Yin; Dachun Yu; Lihong He; Songdong Shen
Ulva prolifera (Ulvophyceae, Chlorophyta), one of the main seaweeds species that cause “green tides”, suffers changes in nitrogen levels during drifting. However, the mechanisms of how this alga resist to nitrogen deficiency remains unclear. Transcripts encoding nitrate reductase, nitrite reductase, and glutamine synthase involved in nitrogen assimilation were amplified by RT-PCR, suggesting that their
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Effects of algae subtype and extraction condition on extracted fucoxanthin antioxidant property: A 20-year meta-analysis Algal. Res. (IF 4.008) Pub Date : 2020-12-18 Shuang Qiu; Yeting Shen; Zhengshuai Wu; Xingchen Zhang; Shijian Ge
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Recent advancements in the genetic engineering of microalgae Algal. Res. (IF 4.008) Pub Date : 2020-12-17 Ashley E. Sproles; Francis J. Fields; Tressa N. Smalley; Chau H. Le; Amr Badary; Stephen P. Mayfield
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Using surfactants for controlling rotifer contamination in mass cultivation of Chlorella pyrenoidosa Algal. Res. (IF 4.008) Pub Date : 2020-12-18 Aoqi Zhang; Xiaobin Wen; Kaixuan Wang; Yan Huo; Yahong Geng; Yi Ding; Yeguang Li
Frequent microzooplankton contamination such as rotifers in Chlorella mass culture often causes devastating effects on biomass production. In this study, five surfactants were used to control contamination by a rotifer, Brachionus calyciflorus, in Chlorella pyrenoidosa XQ-20044 cultures. Laboratory results showed that the minimal effective concentrations of surfactants for complete control of B. calyciflorus
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Comparative study of the impact of conventional and unconventional drying processes on phycobiliproteins from Arthrospira platensis Algal. Res. (IF 4.008) Pub Date : 2020-12-18 Rajaa Seghiri; Jack Legrand; Rachid Hsissou; Azzouz Essamri
The drying process significantly affects the maintaining components offering the high added value of the dried biomass, mainly for the algae industry where the wet biomass is highly perishable. The main objective of this study is to compare the impact of drying processes that are often used in the food industry on phycobiliproteins from Arthrospira platensis. Although total proteins do not show a significant
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Characterization of selected microalgae and cyanobacteria as sources of compounds with antioxidant capacity Algal. Res. (IF 4.008) Pub Date : 2020-12-18 Martin Almendinger; Franziska Saalfrank; Sascha Rohn; Elke Kurth; Monika Springer; Daniel Pleissner
Due to the adaption to environmental stress, biomasses of microalgae and cyanobacteria contain high-value pigments and phenolic compounds with antioxidant capacity to be used as ingredients for food, cosmetics, and pharmaceuticals. To expand the current state of knowledge about microalgae and cyanobacteria as sources of high-value compounds biomasses and extracts of 13 microalgal and cyanobacterial
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Effect of the inclusion of microalgae on the physical properties of extruded feed for gilthead seabream (Sparus aurata L.) Algal. Res. (IF 4.008) Pub Date : 2020-12-17 R. Alcaraz; A. Hernández-Contreras; P. Iglesias; M.D. Hernández
The inclusion of new ingredients in fish feed can affect the physical quality of the pellets. In order to study the effect of the addition of microalgae on the physical properties of the pellets, 15 extruded diets were studied that included three different species of microalgae (Nannochloropsis gaditana, Tetraselmis chuii and Chlorella vulgaris) at five different inclusion levels (0, 2.5, 5, 10 and
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A new algae technical standards focus group: Summarizing and guiding the algae state of the art Algal. Res. (IF 4.008) Pub Date : 2020-12-16 Lieve M.L. Laurens
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Effects of dietary use of two lipid extracts from the microalga Nannochloropsis gaditana (Lubián, 1982) alone and in combination on growth and muscle composition in juvenile gilthead seabream, Sparus aurata Algal. Res. (IF 4.008) Pub Date : 2020-12-16 R. Sales; A. Galafat; A.J. Vizcaíno; M.I. Sáez; T.F. Martínez; M.C. Cerón-García; E. Navarro-López; M.Y. Tsuzuki; F.G. Acién-Fernández; E. Molina-Grima; F.J. Alarcón
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Recycling nutrients from soy sauce wastewater to culture value-added Spirulina maxima Algal. Res. (IF 4.008) Pub Date : 2020-12-13 Pei Han; Qian Lu; Huiqi Zhong; Jiawen Xie; Lijian Leng; Jingjing Li; Liangliang Fan; Jun Li; Paul Chen; Yuping Yan; Feng Wei; Wenguang Zhou
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Comparison of algae growth and symbiotic mechanisms in the presence of plant growth promoting bacteria and non-plant growth promoting bacteria Algal. Res. (IF 4.008) Pub Date : 2020-12-13 Haixin Peng; Luz E. de- Bashan; Brendan T. Higgins
Culturing algae with bacteria has been explored as a means of promoting algal growth and altering algal biochemical composition. Plant growth promoting bacteria (PGPB) are of particular interest given their ability to produce phytohormones that are bioactive in microalgae. However, many non-plant growth promoting bacteria also promote algal growth. The objective of this study was to test the benefits
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Multiscale integration of mixotrophic microalgal cultivation, lipid synthesis, rapid biomass harvesting, and nutrient recycling in pilot-scale photobioreactors Algal. Res. (IF 4.008) Pub Date : 2020-12-10 Arun Kumar Mehta; Saikat Chakraborty
This work presents an acetic acid mediated energy-efficient process that integrates mixotrophic cultivation of extremophilic microalga Chlorella sorokiniana at the photobioreactor (macro) scale, with algal lipid accumulation at the molecular (micro) scale, and rapid biomass harvesting at the algal particle (meso) scale, followed by multiple recycling of nutrient-rich harvested broth. Chlorella sorokiniana
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Novel, automated, semi-industrial modular photobioreactor system for cultivation of demanding microalgae that produce fine chemicals—The next story of H. pluvialis and astaxanthin Algal. Res. (IF 4.008) Pub Date : 2020-12-10 Daniel Borowiak; Paweł Lenartowicz; Michał Grzebyk; Maciej Wiśniewski; Jacek Lipok; Paweł Kafarski
Recently, there has been increased interest in the use of microorganisms in the production of pharmaceuticals, nutraceuticals and energy supply products, which is due to their rapid growth rate and ability to biosynthesize fine chemicals or biotransform specific xenobiotics. To achieve the desired scale of production and optimization of microbial cultures, it is necessary to design bioreactors that
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Effect of temperature, nitrate concentration, pH and bicarbonate addition on biomass and lipid accumulation in the sporulating green alga PW95 Algal. Res. (IF 4.008) Pub Date : 2020-12-10 L. Corredor; E.P. Barnhart; A.E. Parker; R. Gerlach; M.W. Fields
The mixed effects of temperature (20 °C, 25 °C and 30 °C), nitrate concentration (0.5 mM and 2.0 mM), pH buffer, and bicarbonate addition (trigger) on biomass growth and lipid accumulation were investigated in the environmental alga PW95 during batch experiments in standardized growth medium. PW95 was isolated from coal-bed methane production water and classified as a Chlamydomonas-like species by
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Carotenoid composition of locally found seaweeds of Dakshina Kannada district in India Algal. Res. (IF 4.008) Pub Date : 2020-12-10 Ishani Bhat; Gopinath Haripriya; Nishithkumar Jogi; Bangera Sheshappa Mamatha
The alarming consequences of the current day lifestyle practices demand the need for natural metabolites. The recent shift of people's interest in natural therapy over synthetic medicine is gaining popularity. Carotenoids are one such group of pigments produced by photosynthetic organisms that have excellent antioxidant properties. Consumption of carotenoids is recommended for specific health benefits
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Determination of total iodine content in edible seaweeds: Application of inductively coupled plasma-atomic emission spectroscopy Algal. Res. (IF 4.008) Pub Date : 2020-12-09 Jelena Milinovic; Carla Rodrigues; Mário Diniz; João Paulo Noronha
Iodine is an essential element necessary for the synthesis of thyroid hormones, and its deficiency is a common problem worldwide. To avoid iodine insufficiency, the intake of this element must be properly evaluated concerning its adequate intake (AI), which is estimated at 90, 150, and 200 μg per day, for children, adults, and pregnant women, respectively. One of the most abundant resources available
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Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation Algal. Res. (IF 4.008) Pub Date : 2020-12-06 Ling Li; Litao Zhang; Jianguo Liu
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Acid hydrolysis conditions for the production of fine chemicals from Gracilaria birdiae alga biomass Algal. Res. (IF 4.008) Pub Date : 2020-12-05 José Cirlanio Sousa Albuquerque; Márjory Lima Holanda Araújo; Maria Valderez Ponte Rocha; Bartolomeu Warlene Silva de Souza; George Meredite Cunha de Castro; Edna Maria Silva Cordeiro; Jouciane de Sousa Silva; Norma Maria Barros Benevides
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Valorization and upgrading of the nutritional value of seaweed and seaweed waste using the marine fungi Paradendryphiella salina to produce mycoprotein Algal. Res. (IF 4.008) Pub Date : 2020-12-04 Catalina Landeta Salgado; Rosa Muñoz; Adriana Blanco; María Elena Lienqueo
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Development and application of quantitative real-time PCR based on the mitochondrial cytochrome oxidase subunit I gene for early detection of the grazer Poterioochromonas malhamensis contaminating Chlorella culture Algal. Res. (IF 4.008) Pub Date : 2020-11-24 Xianhui Wang; Huannan Li; Xueling Zhan; Mingyang Ma; Danni Yuan; Qiang Hu; Yingchun Gong
Poterioochromonas malhamensis is a flagellate grazer that readily contaminates Chlorella cultures and can have a devastating impact on them, especially in large-scale culture. Here, we developed a quantitative real-time polymerase chain reaction (qPCR) method to detect and quantify P. malhamensis from high-density Chlorella cultures. A set of primers specific to the mitochondrial cytochrome oxidase
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A data-driven comparison of commercially available testing methods for algae characterization Algal. Res. (IF 4.008) Pub Date : 2020-11-24 Madeline Lane; Stefanie Van Wychen; Andy Politis; Lieve M.L. Laurens
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Chemoplasticity of the polar lipid profile of the microalgae Chlorella vulgaris grown under heterotrophic and autotrophic conditions Algal. Res. (IF 4.008) Pub Date : 2020-11-23 Daniela Couto; Tânia Melo; Tiago A. Conde; Margarida Costa; Joana Silva; M. Rosário M. Domingues; Pedro Domingues
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Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515 Algal. Res. (IF 4.008) Pub Date : 2020-11-21 Christian Südfeld; Michal Hubáček; Sarah D'Adamo; René H. Wijffels; Maria J. Barbosa
Background Microalgae are considered a promising platform for sustainable lipid production. Despite this, productivities need to be improved to facilitate an economically viable production process. This can be achieved through strain improvement, for instance by genetic engineering. Strain improvement strategies often deploy high-throughput screening platforms i.a. involving single-cell methodologies
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Low temperatures in dark period affect biomass productivity of a cyanobacterium Arthrospira platensis Algal. Res. (IF 4.008) Pub Date : 2020-11-21 Kenji Tanaka; Masatoshi Kishi; Hirut Assaye; Tatsuki Toda
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Adaptive Laboratory Evolution for algal strain improvement: methodologies and applications Algal. Res. (IF 4.008) Pub Date : 2020-11-20 Alaina J. LaPanse; Anagha Krishnan; Matthew C. Posewitz
Originally developed in bacterial and fungal models, Adaptive Laboratory Evolution (ALE) experimentally selects for targeted phenotypes by allowing cells containing beneficial random genetic mutations to replicate more quickly in the presence of environmental stress. When applied to microalgae, this technique has been used to increase growth rate, stress tolerance, substrate utilization, and product
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Volatile compounds and odour characteristics of five edible seaweeds preserved by high pressure processing: Changes during refrigerated storage Algal. Res. (IF 4.008) Pub Date : 2020-11-19 Olga López-Pérez; Ana del Olmo; Antonia Picon; Manuel Nuñez
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Growth, biostimulant and biopesticide activity of the MACC-1 Chlorella strain cultivated outdoors in inorganic medium and wastewater Algal. Res. (IF 4.008) Pub Date : 2020-11-19 Karolína Ranglová; Gergely Ernö Lakatos; João Artur Câmara Manoel; Tomáš Grivalský; Francisca Suárez Estrella; Francisco Gabriel Acién Fernández; Zoltán Molnár; Vince Ördög; Jiří Masojídek
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Interactive effect of light quality and temperature on Chlamydomonas reinhardtii growth kinetics and lipid synthesis Algal. Res. (IF 4.008) Pub Date : 2020-11-16 Xiangpeng Li; Shelyn Slavens; Daniel W. Crunkleton; Tyler W. Johannes
The efficient operation of an algal photobioreactor (PBR) requires appropriate lighting conditions. Despite recent advantages in PBR design and operation, optimal lighting conditions are still not fully understood. This work focuses on the effect of light quality and culturing temperature on the algal growth kinetics and lipid content. The green alga Chlamydomonas reinhardtii was grown in batch culture
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Enhancing yield on Macrocystis pyrifera (Ochrophyta): The effect of gametophytic developmental strategy Algal. Res. (IF 4.008) Pub Date : 2020-11-14 Alejandro H. Buschmann; Karina Villegas; Sandra V. Pereda; Carolina Camus; José L. Kappes; Robinson Altamirano; Luis Vallejos; María C. Hernández-González
Macrocystis pyrifera gametophytes growing under controlled conditions produce juveniles with different numbers of fronds, i.e. few (≤ 2) or multiple fronds (≥ 3). We describe these morphotypes and test whether the number of fronds may impact juvenile growth by performing laboratory and field experiments. Kelp reproductive material was collected in southern Chile and 133 female gametophytes were individually