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Chloroplastic ascorbate acts as a regulatory hub in plant metabolism regardless of oxidative stress bioRxiv. Plant Biol. Pub Date : 2024-03-18 David Toth, Roland Tengolics, Fayezeh Aarabi, Anna Karlsson, Andre Vidal-Meireles, Laszlo Kovacs, Soujanya Kuntam, Timea Kormoczi, Alisdair R. Fernie, Elton Hudson, Balazs Papp, Szilvia Z. Toth
Ascorbate is a major plant metabolite that plays crucial roles in various processes, from reactive oxygen scavenging to epigenetic regulation. However, to what extent and how ascorbate modulates metabolism is largely unknown. To address this, we investigated the consequences of chloroplastic and total cellular ascorbate-deficiencies by studying chloroplastic ascorbate-transporter pht4;4 mutant lines
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Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta. bioRxiv. Plant Biol. Pub Date : 2024-03-18 Maria Font Farre, Daniel Brown, Reka Toth, Chidambareswaren Mahadevan, Melissa Brazier-Hicks, Kyoko Morimoto, Farnusch Kaschani, John Sinclair, Richard Dale, Samantha Hall, Melloney Morris, Markus Kaiser, Aaron T. Wright, Jonathan Burton, Renier A. L. van der Hoorn
Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when
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High resolution mapping of novel non-transgressive hybrid susceptibility in barley exploited by P. teres f. maculata maps to a single pentatricopeptide repeat-containing protein bioRxiv. Plant Biol. Pub Date : 2024-03-17 Shaun James Clare, Abdullah F Alhashel, Mengyuan Li, Karl M Effertz, Roshan Sharma Poudel, Jianwei Zhang, Robert Saxon Brueggeman
Hybrid genotypes can provide significant yield gains over conventional inbred varieties due to heterosis or hybrid vigor. However, hybrids can also display unintended negative attributes or phenotypes such as extreme pathogen susceptibility. The necrotrophic pathogen Pyrenophora teres f. maculata (Ptm) causes spot form net blotch, which has caused significant losses to barley worldwide. Here, we report
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Effects of arbuscular mycorrhizal fungi on nitrogen uptake in cotton (Gossypium hirsutum L.) under low-nitrogen conditions bioRxiv. Plant Biol. Pub Date : 2024-03-17 Hushan Wang, yijian wang, Xiaojiao cheng, Yunzhu He, zihui shen, Wangfeng zhang, xiaozhen pu
Cotton is an important global cash crop whose yield and quality are highly influenced by soil nitrogen. Therefore, examining the interactions between roots and arbuscular mycorrhizal fungi (AMF) under reduced nitrogen conditions is of great significance. We investigated the effects of nitrogen application (0, 250, and 375 kg*hm-2) on the AMF infection rate of cotton, the nitrogen content of each organ
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The distribution of particulate organic matter in the heterogeneous soil matrix - balancing between aerobic respiration and denitrification bioRxiv. Plant Biol. Pub Date : 2024-03-17 Maik Lucas, Lena Rohe, Bernd Apelt, Claus Florian Stange, Hans-Jörg Vogel, Reinhard Well, Steffen Schlüter
Denitrification, a key process in soil nitrogen cycling, occurs predominantly within microbial hotspots, where denitrifiers use nitrate as an alternative electron acceptor. For accurate prediction of dinitrogen (N2) and nitrous oxide (N2O) emissions from denitrification, a precise quantification of these microscale hotspots is required. Employing a unique combination of X-ray CT imaging, microscale
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Deep learning based genomic breeding of pest-resistant grapevine bioRxiv. Plant Biol. Pub Date : 2024-03-17 Yu Gan, Zhenya Liu, Fan Zhang, Qi Xu, Xu Wang, Hui Xue, Xiangnian Su, Wenqi Ma, Qiming Long, Anqi Ma, Guizhou Huang, Wenwen Liu, Xiaodong Xu, Lei Sun, Yingchun Zhang, Yuting Liu, Xinyue Fang, Chaochao Li, Xuanwen Yang, Pengcheng Wei, Xiucai Fan, Chuan Zhang, Pengpai Zhang, Chonghuai Liu, Zhiwu Zhang, Sanwen Huang, Yiwen Wang, Zhongjie Liu, Yongfeng Zhou
Crop pests have profoundly deleterious effects on crop yield and food security. However, conventional pest control depends heavily on the utilization of insecticides, which develops strong pesticide resistance and concerns of food safety. Crop and their wild relatives display diverse levels of pest resistance, indicating the feasibility for breeding of pest-resistant crop varieties. In this study,
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Transcriptomic analyses in the gametophyte of Dryopteris affinis: apomixis and more bioRxiv. Plant Biol. Pub Date : 2024-03-17 Sara Ojosnegros, Jose Manuel Alvarez, Valeria Gagliardini, Luis G. Quintanilla, Ueli Grossniklaus, Helena Fernandez
The gametophyte of the fern Dryopteris affinis ssp. affinis represents a good model to explore the molecular basis of vegetative and reproductive development, as well as stress responses. Specifically, this fern reproduces asexually by apogamy, a peculiar case of apomixis whereby a sporophyte forms directly from a gametophytic cell without fertilization. Using an RNA-sequencing approach, we have previously
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Hairpin-RNA Spray Confers Resistance to Mungbean Yellow Mosaic India Virus in Mungbean bioRxiv. Plant Biol. Pub Date : 2024-03-17 Kiran Vilas Dhobale, Lingaraj Sahoo
The prevalence of Begomovirus diseases poses a significant threat to legume crops, necessitating the exploration of innovative control measures. This investigation explores the utilization of dsRNA molecules to initiate RNA interference (RNAi) targeting begomovirus, particularly focusing on Mungbean yellow mosaic India virus (MYMIV) and its potential threat to mungbean crops. Given the lack of genetic
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An optimized somatic embryo transformation system assisted homozygous edited rubber tree generation method mediated by CRISPR/Cas9 bioRxiv. Plant Biol. Pub Date : 2024-03-16 Xianfeng Yang, Qiufei Lin, Jinu Udayabhanu, Yuwei Hua, Xuemei Dai, Shichao Xin, Huasun Huang, Tiandai Huang
Previously, we have realized the CRISPR/Cas9-RNP and plasmid mediated protoplast transient transformation genome editing in the rubber tree (Hevea brasiliensis), but no gene editing plants were acquired due to the bottleneck of genetic transformation. In present study, antibiotic sensitivity tests against kanamycin, hygromycin and basta were analyzed for embryo screening, the results demonstrated that
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A bacterial type III effector hijacks plant ubiquitin proteases to evade degradation bioRxiv. Plant Biol. Pub Date : 2024-03-16 Wenjia Yu, Meng Li, Wenjun Wang, Haiyan Zhuang, Jiamin Luo, Yuying Sang, Cecile Segonzac, Alberto P Macho
Gram-negative bacterial pathogens inject effector proteins inside plant cells using a type III secretion system. These effectors manipulate plant cellular functions and suppress the plant immune system in order to promote bacterial proliferation. Despite the fact that bacterial effectors are exogenous threatening proteins potentially exposed to the protein degradation systems inside plant cells, effectors
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Postembryonic developmental roles of the Arabidopsis KEULE gene bioRxiv. Plant Biol. Pub Date : 2024-03-16 Alejandro Ruiz-Bayón, Carolina Cara-Rodríguez, Raquel Sarmiento-Mañús, Rafael Muñoz-Viana, Francisca M. Lozano, María Rosa Ponce, José Luis Micol
Cytokinesis in plant cells begins with the fusion of vesicles that transport cell wall materials at the center of the cell division plane, where the cell plate forms and expands radially until it fuses with the parental cell wall at the preprophase band. Vesicle fusion is facilitated by trans-SNARE complexes, with assistance from Sec1/Munc18 (SM) proteins. The SNARE protein KNOLLE and the SM protein
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Decoding strawberry volatile cultivar diversity through comparative transcriptome analysis bioRxiv. Plant Biol. Pub Date : 2024-03-16 Kondylia Passa, Evangelos Tsormpatsidis, Ioannis Ganopoulos, Christos Bazakos, Vasileios Papasotiropoulos
This study presents a comparative transcriptomic analysis of three commercial strawberry cultivars: Rociera, Calderon, and Victory, aimed at uncovering the molecular basis of their distinct flavor and aroma profiles. Through RNA sequencing, we analyzed the transcriptomic landscape of these varieties, uncovering a notable array of differentially expressed genes (DEGs) between them. Specifically, Rociera
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The transcription factor ATML1 maintains giant cell identity by inducing synthesis of its own (very) long-chain fatty acid-containing ligands bioRxiv. Plant Biol. Pub Date : 2024-03-16 Batthula Vijaya Lakshmi Vadde, Nicholas Joseph Russell, Saket R. Bagde, Bryce Askey, Michael M Saint-Antoine, Bryce A. Brownfield, Salaiha Mughal, Lauren E. Apprill, Aashima Khosla, Frances K. Clark, Erich Marquard Schwarz, Saleh Alseekh, Alisdair R. Fernie, Abhyudai Singh, Kathrin Schrick, J. Christopher Fromme, Aleksandra Skirycz, Pau Formosa-Jordan, Adrienne H. K. Roeder
During development, cells not only adopt specialized identities but also maintain those identities. Endoreduplication is thought to maintain cell identity. High concentrations of ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1) specify giant cell identity and induce endoreduplication in sepals. How different concentrations of ATML1 can specify different identities remains unclear. Here, we show that high
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Graphical pangenomics-enabled characterisation of structural variant impact on gene expression in Brassica napus bioRxiv. Plant Biol. Pub Date : 2024-03-16 Agnieszka A. Golicz, Gozde Yildiz, Sven Weber, Tobias Kox, Amine Abbadi, Rod J. Snowdon, Silvia F. Zanini
Structural variants (SVs, eg. insertions and deletions) are genomic variations > 50 bp that are known to be associated with a range of crop traits, from yield to flowering behaviour and stress responses. Recently, pangenome graphs have emerged as a powerful framework for analysing genomic data by encoding population- or species- level diversity in one data structure. Pangenome graphs have the potential
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The molecular basis of pine wilt disease resistance in Pinus massoniana bioRxiv. Plant Biol. Pub Date : 2024-03-16 jinjin wang, Shouping Cai, Liqiong Zeng, Jie Tong, Danling Hang, Xinliang Zhang, Yu Fang, Shunde Su, JUN SU
Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, results in significant economic and ecological damage to Pinus forests and plantations worldwide. Pinus massoniana is the primary host of PWD in southern China, but its response to the PWN remains largely unstudied. Previously, we observed PWD in a P. massoniana nursery that contained over 71 commonly used cultivars
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Overlapping roles of Arabidopsis INCURVATA11 and CUPULIFORMIS2 as Polycomb Repressive Complex 2 accessory proteins bioRxiv. Plant Biol. Pub Date : 2024-03-15 Riad Nadi, Lucia Juan-Vicente, Samuel Daniel Lup, Yolanda Fernandez, Vicente Rubio, Jose Luis Micol
Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation of lysine 27 of histone H3 (H3K27me3) and plays a key role in epigenetic repression of gene expression in plants and animals. PRC2 core components have all been identified in Arabidopsis thaliana, with an expanding list of accessory proteins, some of which facilitate the recruitment of PRC2 to specific targets. INCURVATA11 (ICU11) is
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Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM bioRxiv. Plant Biol. Pub Date : 2024-03-15 Leander Rohr, Alexandra Ehinger, Nina Glöckner Burmeister, Alfred J Meixner, Birgit Kemmerling, Klaus Harter, Sven zur Oven-Krockhaus
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the application of dynamic live-cell methods in plants has been limited by the lack of suitable fluorophores for simultaneous visualization of multiple proteins. To address this challenge, we implemented a two-color
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A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves, suitable for large-scale assessment of resistance in poplar breeding progeny bioRxiv. Plant Biol. Pub Date : 2024-03-15 zheng li, Bingyu Zhang, Yuchen Fu, Yutian Suo, Yinan Zhang, Jinxia Feng, Long Pan, Wanna Shen, Huixiang Liu, Xiaohua Su, Jiaping Zhao
Hybrid breeding is the most direct and efficient method of controlling and managing tree diseases. However, "in vitro stem inoculation" can not be used for rapid, efficient, and low-cost screening of resistant clones in the early stage of stem canker infection. Therefore, we inoculated stem canker pathogens on poplar leaves to evaluate the resistance of hybrid clones in the poplar-Valsa sordida pathosystem
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Performance of localization prediction algorithms decreases rapidly with the evolutionary distance to the training set increasing bioRxiv. Plant Biol. Pub Date : 2024-03-15 Sven B. Gould, Jonas Magiera, Carolina Garcia Garcia, Parth K Raval
Mitochondria and plastids import thousands of proteins. Their experimental localisation remains a frequent task, but can be resource-intensive and sometimes impossible. Hence, hundreds of studies make use of (machine learning) algorithms that predict a sub-cellular localisation of a protein based its sequence. Their reliability across evolutionary diverse species is unknown. Here, we evaluate the performance
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Unveiling shared genetic regulators for plant architectural and biomass yield traits in sorghum bioRxiv. Plant Biol. Pub Date : 2024-03-14 Anuradha Singh, Linsey A Newton, James C Schnable, Addie M. Thompson
Sorghum is emerging as an ideal genetic model for designing high-biomass bioenergy crops. Biomass yield, a complex trait influenced by various plant architectural features, is typically regulated by numerous genes. This study aims to dissect the genetic mechanisms underlying fourteen plant architectural and ten biomass yield traits in a sorghum association panel (SAP) across two growing seasons. We
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Natural soil suppressiveness against soilborne phytopathogens extends to the control of insect pest bioRxiv. Plant Biol. Pub Date : 2024-03-14 Nadine Harmsen, Pilar Vesga, Gaétan Glauser, Françoise Klötzli, Clara Margot Heiman, Aline Altenried, Jordan Vacheron, Daniel Muller, Yvan Moënne-Loccoz, Thomas Steinger, Christoph Keel, Daniel Garrido-Sanz
Since the 1980s, soils in a 22-km2 area near Lake Neuchâtel in Switzerland have been recognized for their innate ability to suppress the black root rot plant disease. Their efficacy against insect pests has not been studied. We demonstrate that natural soil suppressiveness also protects plants from the leaf-feeding pest insect Oulema melanopus. Plants grown in the most suppressive soil have a reduced
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Automated Seminal Root Angle Measurement with Corrective Annotation bioRxiv. Plant Biol. Pub Date : 2024-03-14 Abraham George Smith, Marta Malinowska, Anja Karine Ruud, Luc Janss, Lene Krusell, Jens Due Jensen, Torben Asp
Measuring seminal root angle is an important aspect of root phenotyping, yet automated meth- ods are lacking. We introduce SeminalRootAngle, a novel open-source automated method that measures seminal root angles from images. To ensure our method is flexible and user-friendly we build on an established corrective annotation training method for image segmentation. We tested SeminalRootAngle on a heterogeneous
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The GPAT4/6/8 clade functions in Arabidopsis root suberization non-redundantly with the GPAT5/7 clade required for suberin lamellae bioRxiv. Plant Biol. Pub Date : 2024-03-14 Kay Gully, Alice Berhin, Damien de Bellis, Cornelia Herrfurth, Ivo Feussner, Christiane Nawrath
Lipid polymers such as cutin and suberin strengthen the diffusion barrier properties of the cell wall in specific cell types and are essential for water relations, mineral nutrition, and stress protection in plants. Land plant-specific glycerol-3-phosphate acyltransferases (GPATs) of different clades are central players in cutin and suberin monomer biosynthesis. Here, we show that the GPAT4/6/8 clade
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Viewing stomata in action: Autonomous in planta imaging of individual stomatal movement links morphology and kinetics bioRxiv. Plant Biol. Pub Date : 2024-03-14 Tomas E van den Berg, Remco G P Sanders, Elias Kaiser, Jurriaan Schmitz
Stomata regulate plant gas exchange with the environment, balancing between water loss and CO2 uptake. Gas exchange dynamics are influenced by traits such as stomatal morphology, size and density, which are commonly investigated using imprints and manual microscopy, methods that are destructive and time-consuming. Moreover, these microscopic properties are statically sampled and related to the dynamic
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The Pivotal Involvement of the Respiratory burst oxidase G (SlRbohG) Gene in H2O2 Production Under Stress for Proper Na+ Homeostasis Regulation in Tomato bioRxiv. Plant Biol. Pub Date : 2024-03-14 Isabel Egea, Teresa Barragan-Lozano, Yanira Estrada, Maribel Jáquez-Gutiérrez, Feliz A Plasencia, Alejandro Atares, Begoña Garcia-Sogo, Carmen Capel, Fernando J Yuste-Lisbona, Jose M Egea-Sánchez, Francisco Borja-Flores, Trinidad Angosto, Vicente Moreno, Rafael Lozano, Benito Pineda
Regulation of sodium homeostasis is crucial for plant response to salinity conditions. Here we report on the genetic and physiological characterization of two tomato allelic mutants, sodium gatherer1-2 (sga1-2), which exhibit pronounced chlorosis and hyperhydration under salt stress. Mapping-by-sequencing revealed that mutant phenotype resulted from mutations in the SlRbohG gene, and CRISPR/Cas9 knockouts
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Enabling Greenhouse Gas Emission Reduction while Improving Rice Yield with a Methane-Derived Microbial Biostimulant bioRxiv. Plant Biol. Pub Date : 2024-03-14 Sarma Rajeev Kumar, Einstein Mariya David, Gangigere Jagadish Pavithra, Gopalakrishnan Kumar Sajith, Kuppan Lesharadevi, Selvaraj Akshaya, Chavaddi Bassavaraddi, Gopal Navyashree, Panakanahalli Shivaramu Arpitha, Padmanabhan Sreedevi, Khan Zainuddin, Firdous Saiyyeda, Bondalakunta Ravindra Babu, Muralidhar Udagatti Prashanth, Ganesan Ravikumar, Palabhanvi Basavaraj, Chavana Sandeep Kumar, Vinod Munisanjeeviah
Rice is a vital crop for food security and human nutrition, yet its cultivation produces ~11% of total global anthropogenic methane (CH4) emissions - the second most important greenhouse gas (GHG). Modifications to rice management practice are necessary, both to increase yield and mitigate GHG emissions. We investigated the effect of a methane-derived microbial biostimulant on grain yield and GHG emissions
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Molecular signatures and associated regulators of the pea leaf response to sulfur deficiency and water deficit as revealed by multi-omics analyses bioRxiv. Plant Biol. Pub Date : 2024-03-14 Titouan Bonnot, Charlotte Henriet, Delphine Aimé, Jonathan Kreplak, Morgane Térézol, Thierry Balliau, Alain Ourry, Michel Zivy, Vanessa Vernoud, Karine Gallardo
Sulfur availability in soils affects both yield and seed quality in major crops, and the plant capacity to tolerate environmental constraints. Under stress combination, plants often show specific responses at the molecular level. To dissect the molecular responses to sulfur deficiency in interaction or not with water deficit, a multi-omics approach was used focusing on the leaves of pea (Pisum sativum)
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Stem rot affects the structure of rhizosphere microbiome in Berseem Clover (Trifolium alexandrinum) bioRxiv. Plant Biol. Pub Date : 2024-03-14 Salma Mukhtar, Zain Ahmad, Noor Khan, Michael John, Dalaq Aiysha
Rhizosphere microbiome plays an essential role in maintaining plant health and productivity. Fungal and bacterial diseases may affect the rhizosphere-associated microbial communities and overall structure of plant microbiome. Here, we studied the effect of stem rot of berseem clover on the bacterial and fungal communities associated with the rhizosphere. We analyzed the rhizosphere-associated bacterial
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Genome-wide association studies in a diverse strawberry collection unveil loci controlling agronomic and fruit quality traits bioRxiv. Plant Biol. Pub Date : 2024-03-13 Pilar Munoz, F. Javier Roldan-Guerra, Sujeet Verma, Mario Ruiz-Velazquez, Rocio Torreblanca, Nicolas Oiza, Cristina Castillejo, Jose F. Sanchez Sevilla, Iraida Amaya
Strawberries are cherished for their organoleptic properties and nutritional value. However, breeding new cultivars involves the simultaneous selection of many agronomical and fruit quality traits. The strawberry germplasm collection here studied exhibited extensive phenotypic variation in 26 agronomic and fruit quality traits across three consecutive seasons. Phenotypic correlations and Principal
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Large gradient of susceptibility to esca disease revealed by long-term monitoring of 46 grapevine cultivars in a common garden vineyard bioRxiv. Plant Biol. Pub Date : 2024-03-13 Pierre Gastou, Agnes Destrac Irvine, Clarisse Arcens, Eva Courchinoux, Patrice This, Cornelis van Leeuwen, Chloe E. L. Delmas
Grapevine (Vitis vinifera L.) is prone to many fungal diseases, including esca, a severe vascular disease threatening the wine sector for which there is no cost-effective cure. Susceptibility to esca varies between cultivars in different infection conditions. It may therefore be possible to use the genetic diversity of grapevine cultivars to mitigate disease impact. However, the genetic component of
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Separate domains of the Arabidopsis ENHANCER OF PINOID drive its own polarization and recruit PIN1 to the plasma membrane bioRxiv. Plant Biol. Pub Date : 2024-03-13 Ramon Torres, Michaela Matthes, Nicole Yun, Miriam Luichtl, Ulrich Büschges, Birgit Fiesselmann, Benjamin Strickland, Marietta Lehnardt
The Arabidopsis ENHANCER OF PINOID (ENP) protein and the AGC-kinase PINOID (PID) synergistically impact on polarization of the auxin transporter PIN-FORMED1 (PIN1) required for plant leaf and flower organ development. ENP offers a PID-independent input for PIN-polarity since enp pid double mutants lead to cotyledon- and flower-less plants in contrast to pid single mutants, which develop cotyledons
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Redirecting DNA repair for efficient CRISPR-Cas-based gene targeting in tomato bioRxiv. Plant Biol. Pub Date : 2024-03-13 Tien Van Vu, Ngan Thi Nguyen, Jihae Kim, Minh Huy Vu, Young Jong Song, Mil Thi Tran, Yeon Woo Sung, Jae-Yean Kim
The CRISPR-Cas-based gene targeting (GT) method has enabled precise modifications of genomic DNA ranging from single base to several kilobase scales through homologous recombination (HR). In plant somatic cells, canonical nonhomologous end-joining (cNHEJ) is the predominant mechanism for repairing double-stranded breaks (DSBs), thus limiting the HR-mediated GT. In this study, we implemented various
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Why the adventitious roots of poplar are so colorful: RNAseq and metabolomic analysis reveal flavonols, flavones, and anthocyanins accumulation in canker pathogens-induced adventitious roots in poplar bioRxiv. Plant Biol. Pub Date : 2024-03-13 Li Min, Fu Yuchen, Li Jinxin, Shen Wanna, Wang Li, Li Zheng, Zhang Shiqi, Liu Huixiang, Su Xiaohua, Zhao Jiaping
Recently, we observed a novel allometry on poplar stems, with copious colorful adventitious roots (ARs) induced by fungal canker pathogens. Here, we reveal chemical, physiological, and molecular mechanisms of AR coloration in a poplar-pathogen (Valsa sordida/Botrosphaeria dothidea) interaction system using our phloem girdling-inoculation system. Light-induced coloration in ARs: red/rosy under sunlight
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An array of Zymoseptoria tritici effectors suppress plant immune responses bioRxiv. Plant Biol. Pub Date : 2024-03-13 Elisha Thynne, Haider Ali, Kyungyong Seong, Mohammad Abukhalaf, Marco A Guerreiro, Victor M Flores-Nunez, Rune Hansen, Maja Salman, Jason J Rudd, Kostya Kanyuka, Ksenia V Krasileva, Graeme J Kettles, Eva H Stukenbrock
Zymoseptoria tritici is the most economically significant fungal pathogen of wheat in Europe. However, despite the importance of this pathogen, the molecular interactions between pathogen and host during infection are not well understood. Herein, we describe the use of two libraries of cloned Z. tritici effectors that were screened to identify effector candidates with putative pathogen associated molecular
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Growth arrest is a DNA damage protection strategy in plants bioRxiv. Plant Biol. Pub Date : 2024-03-13 Antonio Serrano-Mislata, Jorge Hernandez-Garcia, Carlos de Ollas, Noel Blanco-Tourinan, Aurelio Gomez-Cadenas, Robert Sablowski, David Alabadi, Miguel A Blazquez
When exposed to stress, plants slow down their growth while activating defensive mechanisms. This behaviour has been proposed to help plants reallocate resources and meet the energy demands required for survival. In this study, we show instead that plants can grow under limited water availability without compromising their tolerance to the stress. However, cells that continue to divide under stress
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De novo genome assembly of Myricaria laxiflora provides insights into its molecular mechanisms under flooding stress bioRxiv. Plant Biol. Pub Date : 2024-03-13 Xiang Weibo, Li Linbao, Huang Guiyun, Dun Bicheng, Chen Huiyuan, Ma Xiaobo, Zhang Haibo, Xiao Zhiqiang, Liu Jihong, Yang Zhen, Wu Di
M. laxiflora is an endangered plant distributed in the Yangtze River floodplain of China, which often suffers from flood hazards during its growth and development. Due to the lack of a reference genome for M. laxiflora, the molecular regulatory mechanism of its waterlogging stress remains unclear. Here, we report for the first time the high-quality reference genome of M. laxiflora by using HiFi sequencing
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Genome-Wide Identification of G-Quadruplex forming regions in Arabidopsis: Unraveling the Role of Pif like Helicase (AtPLH1) in Gene Regulation and Stress Response bioRxiv. Plant Biol. Pub Date : 2024-03-13 Surabhi Singh, Shubhangi Singh, Khushboo Gupta, Himanshi Sharma, Shivsam Bady, Manushka Sondhi, Rohini Garg
G-quadruplexes (GQSes) are highly stable DNA secondary structures, which exist as knots in the genome during different cellular processes like replication, transcription and translation. Although several studies have shown the role of GQS-helicases regulating several cellular processes in yeast and human, their detailed characterization in plants is still lacking. In this study, we identified GQS-enriched
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A high resolution model of the grapevine leaf morphospace predicts synthetic leaves bioRxiv. Plant Biol. Pub Date : 2024-03-13 Daniel H Chitwood, Efrain Torres-Lomas, Ebi S Hadi, Wolfgang L G Peterson, Mirjam F Fischer, Sydney E Rogers, Chuan He, Michael G. F. Acierno, Shintaro Azumaya, Seth Wayne Benjamin, Devendra Prasad Chalise, Ellice E Chess, Alex J Engelsma, Qiuyi Fu, Jirapa Jaikham, Bridget M. Knight, Nikita S Kodjak, Adazsofia Lengyel, Brenda L Muñoz, Justin T Patterson, Sundara I Rincon, Francis L Schumann, Yujie
Grapevine leaves are a model morphometric system. Sampling over ten thousand leaves using dozens of landmarks, the genetic, developmental, and environmental basis of leaf shape has been studied and a morphospace for the genus Vitis predicted. Yet, these representations of leaf shape fail to capture the exquisite features of leaves at high resolution. We measure the shapes of 139 grapevine leaves using
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A fungal transcription factor BOT6 facilitates the transition of a beneficial root fungus into an adapted anthracnose pathogen bioRxiv. Plant Biol. Pub Date : 2024-03-13 Ren Ujimatsu, Junya Takino, Masami Nakamura, Hiromi Haba, Atsushi Minami, Kei Hiruma
The infection strategies employed by plant endophytes are attributed to their ability to overcome durable nonhost resistance and adapt to the host environment. However, the regulatory genetic background underlying how they adapt to the host and determine their lifestyles remains enigmatic. Here, we show that the CtBOT6, a cluster-residing transcription factor in the root-associated fungus Colletotrichum
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Phylogenetically distant enzymes localized in cytosol and plastids drive citral biosynthesis in lemongrass bioRxiv. Plant Biol. Pub Date : 2024-03-13 Priyanka Gupta, Anuj Sharma, N.R. Kiran, T.K. A Pranav Raj, Ram Krishna, Dinesh A Nagegowda
Except for the genetic basis of citral-forming alcohol dehydrogenases (ADHs) in Litsea cubeba tree, and biochemical studies on citral-forming enzymes from select plants, knowledge regarding in-planta biosynthesis of citral and its metabolic origin remains limited. Here, we have elucidated the functions of an ADH (CfADH1) and an aldoketo-reductase (CfAKR2b) in citral biosynthesis in lemongrass (Cymbopogon
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Defense-related callose synthase PMR4 promotes root hair callose deposition and adaptation to phosphate deficiency in Arabidopsis thaliana bioRxiv. Plant Biol. Pub Date : 2024-03-12 Kentaro Okada, Koei Yachi, Tan Anh Nhi Nguyen, Satomi Kanno, Shigetaka Yasuda, Haruna Tadai, Chika Tateda, Tae-Hong Lee, Uyen Nguyen, Kanako Inoue, Shunsuke Miyashima, Kei Hiruma, Kyoko Miwa, Takaki Maekawa, Michitaka Notaguchi, Yusuke Saijo
Plants acquire phosphorus (P) primarily as inorganic phosphate (Pi) from the soil. Under Pi deficiency, plants induce an array of physiological and morphological responses, termed phosphate starvation response (PSR), thereby increasing Pi acquisition and use efficiency. However, the mechanisms underlying PSR remain poorly understood. Here, we report that deposition of a β-1,3-glucan polymer called
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WITHDRAWN: Understanding Regeneration: investigating the apical notch and meristem re-emergence in Marchantia polymorpha bioRxiv. Plant Biol. Pub Date : 2024-03-12 Alan O Marron, Marius Rebmann, Mihails Delmans, Jim O Haseloff
The authors have requested that this manuscript is withdrawn pending revisions to the text and amendments to the authorship list. Therefore, the authors do not wish this current version to be cited as reference for the project. If you have any questions, please contact the corresponding author.
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The unexplored diversity of wild lupins provides rich genomic resources and insights into lupin evolution bioRxiv. Plant Biol. Pub Date : 2024-03-12 Karolina Susek, Edoardo Franco, Magdalena Tomaszewska, Magdalena Kroc, Humaira Jamil, Umesh Tanwar, Matthew N. Nelson, Roberto Papa, Massimo Delledonne, Scott A. Jackson
Lupin crops provide nutritious seeds as an excellent source of dietary protein. However, extensive genomic resources are needed for the adaptation of lupin crops, particularly to improve their nutritional value and facilitate their adaptation to harsh environments caused by the changing climate. Such resources can be derived from crop wild relatives, which represent a large untapped source of genetic
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Comparative transcriptomic and lipidomic analysis of fatty acid accumulation in three Camellia oleifera varieties during maturation bioRxiv. Plant Biol. Pub Date : 2024-03-12 dayu Yang, Rui Wang, Hanggui Lai, Yongzhong Chen, Yimin He, Chengfeng Xun, Ying Zhang, Zhilong He
Camellia oleifera stands out as one of China's leading woody oil crops, famed for its tea oil, which boasts a high content of unsaturated fatty acids. Often referred to as "liquid gold" or "Oriental olive oil," this oil is prized for its nutritional benefits and culinary versatility, marking Camellia oleifera as a valuable agricultural and economic resource. This study provides an integrated investigation
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Bridging fungal resistance and plant growth through constitutive overexpression of Thchit42 gene in Pelargonium graveolens bioRxiv. Plant Biol. Pub Date : 2024-03-12 Laiq Ur Rahman, Kahkashan Khatoon, Zafar Iqbal Warsi, Akanksha Singh, Kajal Singh, Feroz Khan, Palak Singh, Rakesh Shukla, Ramswaroop Verma, Munmun Singh, Sanjeet Kumar Verma, Zakir Husain, Gazala Parween, Pooja Singh, Shama Azmi
Pelargonium graveolens essential oil possesses significant attributes, known for perfumery and aromatherapy. However, optimal yield and propagation are predominantly hindered by biotic stress. All biotechnological approaches have yet to prove effective in addressing fungal resistance. The current study developed transgenic geranium bridging molecular mechanism of fungal resistance and plant growth
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The Zinc Finger protein SlZFP2 is essential for tomato fruit locular tissue morphogenesis bioRxiv. Plant Biol. Pub Date : 2024-03-12 Gabriel Hoang, Joana Jorly, Dario Constantinescu, Pascal GP Martin, Stephanie Gadin, Jean-Philippe Mauxion, Cecile Bres, Virginie Garcia, Nathalie Gonzalez, Christophe Rothan, Nadia Bertin, Lucie Fernandez-Lochu, Martine Lemaire-Chamley
In tomato (Solanum lycopersicum L.) fruit, the locular tissue (LT) is a unique jelly-like tissue that differentiates from the central axis of the fruit after ovule fertilization. LT is essential for seed development and dispersal by preventing early germination and initiating fruit ripening. In this work, we studied a "gel-less" mutant and identified the underlying mutation in the coding sequence of
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Rice OsPLATZ3 controls ROS homeostasis by inhibiting ROS-Scavenging Activity during Tapetum Degeneration bioRxiv. Plant Biol. Pub Date : 2024-03-12 Yuanya Li, Jing Wang, Fengxian Tang, Lin Li, Xingyu Cheng, Xialing Sun, Shuangshuang Yu, Pan Xia, Yuxiang Wang, Mingyang Tong, Lizhong Cheng
OsPLATZ3, a transcription factor of rice (Oryza sativa) belonging to the PLATZ family, is involved in the repression of transcriptional activity. PLATZ3 exhibits preferential expression in the tapetal cells during anthers development, regulating tapetum degeneration by controlling the dynamics of reactive oxygen species (ROS) in rice male reproductive processes. The Osplatz3 exhibits enlarged tapetum
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MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition bioRxiv. Plant Biol. Pub Date : 2024-03-11 David Molina, Sara Horvath, Xudong Zhang, Wei Xiao, Noah Ragab, Dagmar Ripper, Joachim Kilian, Tonni Grube Andersen, Laura Ragni
Plants have developed specialized barriers to protect and isolate the inner tissues from the environment while maintaining homeostasis. Different barriers are present in various organs and at different growth stages. During secondary growth, the periderm acts as the protective tissue, covering roots, stems, and branches as they become thick. The periderm is a dynamic barrier comprising a stem cell
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Hypsochromic shift of phyC complements the inhibition of hypocotyl elongation under moderate red/far-red light bioRxiv. Plant Biol. Pub Date : 2024-03-11 Shizue Yoshihara, Koji Okajima, Satoru Tokutomi
Phytochrome (phy) is a plant photoreceptor that regulates various photomorphogenesis, and occurs in two forms, a red light (R)-absorbing form (Pr) and a far-red light (FR)-absorbing form (Pfr). Absorption spectral analyses of the photosensory module (PSM) showed that phyC in the Pr of Arabidopsis thaliana, Solanum lycopersicum and Zea mays exhibited the absorption maxima shift toward shorter wavelengths
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Stromal DUF760-1 and DUF760-2 proteins are degraded by the chloroplast Clp protease system but have very different half-lives bioRxiv. Plant Biol. Pub Date : 2024-03-11 Bingjian Yuan, Klaas J van Wijk
The chloroplast AAA+ chaperone CLPC1 aids to select, unfold and deliver hundreds of proteins to the stromal CLP protease core complex for degradation. Through in vivo CLPC1 trapping we previously identified dozens of trapped proteins that are substrate adaptors (e.g. CLPS1 and CLPF), other chaperones (CLPC2 and CLPD) or (potential) substrates (e.g. RH3) for the CLP chaperone-protease system. Here we
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XopM, a FFAT motif containing type-III effector protein from Xanthomonas, suppresses PTI responses at the plant plasma membrane bioRxiv. Plant Biol. Pub Date : 2024-03-11 Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Suayib Uestuen, Frederik Boernke
Many Gram-negative pathogenic bacteria use type-III effector proteins (T3Es) as essential virulence factors to suppress host immunity and to cause disease. However, in many cases the molecular function of T3Es remains unknown. The plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) is the causal agent of bacterial spot disease on tomato and pepper plants and is known to translocate around 30
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The activation of Chlamydomonas reinhardtii alpha amylase 2 by glutamine requires its N-terminal ACT domain bioRxiv. Plant Biol. Pub Date : 2024-03-11 Lisa Marie Scholtysek, Ansgar Poetsch, Eckhard Hofmann, Anja Christine Hemschemeier
The coordination of assimilation pathways for all the elements that make up cellular components is a vital task for every organism. Integrating the assimilation and use of carbon (C) and nitrogen (N) is of particular importance because of the high cellular abundance of these elements. Starch is one of the most important storage polymers of photosynthetic organisms, and a complex regulatory network
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A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment bioRxiv. Plant Biol. Pub Date : 2024-03-11 Yi Ding, Virginie Gasciolli, Laurena Medioni, Megane Gaston, Annelie de-Regibus, Celine Rembliere, Jean-Jacques Bono, Julie Cullimore, Marion Dalmais, Christine Saffray, Solene Mazeau, Abdelhafid Bendahmane, Richard Sibout, Michiel Vandenbussche, Jacques Rouster, Tongming Wang, Guanghua He, Arnaud Masselin, Sylvain Cottaz, Sebastien Fort, Benoit Lefebvre
Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High affinity LCO receptors belonging to the LysM receptor-like kinase (LysM-RLK) phylogenetic group LYR-IA play a role in AM establishment, but no plant high affinity short-chain
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Transcriptional repression of GTL1 under water-deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance bioRxiv. Plant Biol. Pub Date : 2024-03-11 Noel Anthony Mano, Mearaj A. Shaikh, Joshua R Widhalm, Chan Yul Yoo, Michael V. Mickelbart
The transcription factor GT2-LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water-limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential role in other water-deficit responses is unknown. We hypothesized that GTL1 regulates transcriptome changes associated
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The Rhizobial effector NopT targets Nod factor receptors to regulate symbiosis in Lotus japonicus bioRxiv. Plant Biol. Pub Date : 2024-03-11 Hanbin Bao, wang yanan, Haoxing Li, Qiang Wang, Yutao Lei, Ying Ye, Hui Zhu, Gary Stacey, Shutong Xu, Yangrong Cao
It is well-studied that type-III effectors are essential required by gram-negative bacteria to directly target different cellular pathways from hosts, thus enhancing their infection. However, in the unique context of legume-rhizobium symbiosis, the role of rhizobial effectors in regulating plant symbiotic pathways remains a subject of hypothesized understanding. Here, we show that NopT, a YopT-type
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Manipulating condensation of thermo-sensitive SUF4 protein tunes flowering time in Arabidopsis thaliana bioRxiv. Plant Biol. Pub Date : 2024-03-10 Heather M Meyer, Takashi Hotta, Andrey Malkovskiy, Yixian Zheng, David W Ehrhardt
Intrinsically disordered proteins (IDP) lack stable tertiary structures, which allows them to change conformation and function under different physicochemical conditions. This may be highly advantageous for plants, which often use changes in their environment to elicit a variety of responses, including developmental events. For instance, some plants delay flowering in the fall and require exposure
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Phylogenomics defines Streptofilum as a novel deep branch of streptophyte algae bioRxiv. Plant Biol. Pub Date : 2024-03-10 Vojtěch Žárský, Marek Eliáš
Streptophytes constitute a major organismal clade comprised of land plants (embryophytes) and several related green algal lineages. Their seemingly well-studied phylogenetic diversity was recently enriched by the discovery of Streptofilum capillaum, a simple filamentous alga forming a novel deep streptophyte lineage in a two-gene phylogeny. A subsequent phylogenetic analysis of plastid genome-encoded
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Unravelling the Interplay of Nitrogen Nutrition and the Botrytis cinerea pectin lyase BcPNL1 in Modulating Arabidopsis thaliana Susceptibility bioRxiv. Plant Biol. Pub Date : 2024-03-09 Antoine Daviere, Aline Voxeur, Sylvie Jolivet, Samantha Vernhettes, Marie-Christine Soulie, Mathilde Fagard
In this study, we investigated the intricate interplay between nitrogen nutrition, and the dynamics of pectin degradation during plant-pathogen interactions, using Arabidopsis thaliana and Botrytis cinerea as a model pathosystem. Our findings revealed a noteworthy impact of nitrogen availability on the pectin degrading activity of the B. cinerea pectin lyase 1 (PNL) for which the mutant strains presented
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Far-red light increases maize volatile emissions in response to volatile cues from neighboring plants bioRxiv. Plant Biol. Pub Date : 2024-03-09 Rocío Escobar-Bravo, Bernardus CJ Schimmel, Yaqin Zhang, Lei Wang, Christelle AM Robert, Gaétan Glauser, Carlos L. Ballaré, Matthias Erb
Plants perceive the presence and defense status of their neighbors through light and volatile cues, but how plants integrate both stimuli is poorly understood. We investigated if and how low Red to Far red light (R:FR) ratios, indicative of shading or canopy closure, affects maize (Zea mays) responses to herbivore-induced plant volatiles (HIPVs), including the green leaf volatile (Z)-3-hexenyl acetate
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Floral scent emission of Epiphyllum oxypetalum: identification of a novel cytosol-localized geraniol biosynthesis pathway bioRxiv. Plant Biol. Pub Date : 2024-03-09 Yiyang Zhang, Yuhan Zhang, Qiurui Tian, Likun Wei, Ting Zhu, Zhiwei Zhou, Jiaqi Wang, Zhibin Liu, Wei Tang, Haijun Xiao, Mingchun Liu, Tao Li, Qun Sun
Epiphyllum oxypetalum, a renowned ornamental species in Cactaceae, releases attractive fragrance during its infrequent, transient and nocturnal flowering, the nature and biosynthesis of the volatiles for this floral scent, however, remained unexplored. Employing volatilomic, transcriptomic and biochemical approaches, we systematically characterized the composition, emission dynamics and biosynthesis