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Targeted editing of tomato carotenoid isomerase reveals the role of 5′ UTR region in gene expression regulation Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-15 K. Lakshmi Jayaraj, Nitu Thulasidharan, Aju Antony, Moni John, Rehna Augustine, Navajeet Chakravartty, Smitha Sukumaran, M. Uma Maheswari, Sweety Abraham, George Thomas, V. B. Reddy Lachagari, Somasekar Seshagiri, Subhash Narayanan, Boney Kuriakose
Key Message A deletion created by CRISPR/Cas9 system in the 5’ UTR of the carotenoid isomerase gene in tomato leads to downregulation of the gene resulting in the low conversion of prolycopene to lycopene. Abstract CRISPR/Cas9 based genome editing is an effective and useful tool adopted from the bacterial immune response system for altering specific, pre-determined DNA sequences in eukaryotes. Such
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The root growth reduction in response to mechanical stress involves ethylene-mediated microtubule reorganization and transmembrane receptor-mediated signal transduction in Arabidopsis Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-13 Takashi Okamoto, Shogo Takatani, Hiroyasu Motose, Hidetoshi Iida, Taku Takahashi
Key message We found that mutations in a Ca 2+ -permeable mechanosensitive channel MCA1, an ethylene-regulated microtubule-associated protein WDL5, and a versatile co-receptor BAK1 affect root growth response to mechanical stress. Abstract Plant root tips exposed to mechanical impedance show a temporal reduction in the elongation growth. The process involves a transient Ca2+ increase in the cytoplasm
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Identification and analysis of a differentially expressed wheat RING-type E3 ligase in spike primordia development during post-vernalization Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-10 Jae Ho Kim, Irfan Ullah Khan, Cheol Won Lee, Dae Yeon Kim, Cheol Seong Jang, Sung Don Lim, Yong Chan Park, Ju Hee Kim, Yong Weon Seo
Key message We identified a RING-type E3 ligase (TaBAH1) protein in winter wheat that targets TaSAHH1 for degradation and might be involved in primordia development by regulating targeted protein degradation. Abstract Grain yield per spike in wheat (Triticum aestivum), is mainly determined prior to flowering during mature primordia development; however, the genes involved in primordia development have
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Base editing in rice: current progress, advances, limitations, and future perspectives Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-10 Rajesh Yarra, Lingaraj Sahoo
Key message Base editing is one of the promising genome editing tools for generating single-nucleotide changes in rice genome. Abstract Rice (Oryza sativa L.) is an important staple food crop, feeding half of the population around the globe. Developing new rice varieties with desirable agronomic traits is necessary for sustaining global food security. The use of genome editing technologies for developing
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Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-07 Urooj Rashid, Humaira Yasmin, Muhammad Nadeem Hassan, Rabia Naz, Asia Nosheen, Muhammad Sajjad, Noshin Ilyas, Rumana Keyani, Zahra Jabeen, Saqib Mumtaz, Mohammed Nasser Alyemeni, Parvaiz Ahmad
Key message A detailed study of the response of wheat plants, inoculated with drought-tolerant PGPR is studied which would be beneficial to achieve genetic improvement of wheat for drought tolerance. Abstract Drought stress, a major challenge under current climatic conditions, adversely affects wheat productivity. In the current study, we observed the response of wheat plants, inoculated with drought-tolerant
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Improving ATPase and PPase activities, nutrient uptake and growth of salt stressed ajowan plants by salicylic acid and iron-oxide nanoparticles Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-05 Kazem Ghassemi-Golezani, Soheila Abdoli
Key message Salicylic acid and iron-oxide nanoparticles alleviated salt toxicity and improved plant growth by stimulating the activities of H+-ATPase and H+-PPase and preventing nutrient imbalance. Abstract Two factorial experiments were undertaken in a greenhouse during 2018 and 2019, to evaluate the impacts of SA (1 mM) and nano-Fe2O3 (3 mM) sprays at 7 leaves and flowering stages on vacuolar H+-pumps
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Production of ORF8 protein from SARS-CoV-2 using an inducible virus-mediated expression system in suspension-cultured tobacco BY-2 cells Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-05 Tomohiro Imamura, Noriyoshi Isozumi, Yasuki Higashimura, Shinya Ohki, Masashi Mori
Key message Production of ORF8 protein from SARS-CoV-2 in tobacco BY-2 cells.
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Recognition of CCA1 alternative protein isoforms during temperature acclimation Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-04 Shijia Zhang, Huili Liu, Li Yuan, Xiaojing Li, Lingbao Wang, Xiaodong Xu, Qiguang Xie
Key message CCA1α and CCA1β protein variants respond to environmental light and temperature cues, and higher temperature promotes CCA1β protein production and causes its retention detectable in the cytoplasm. Abstract CIRCADIAN CLOCK ASSOCIATED1 (CCA1), as the core transcription factor of circadian clock, is involved in the regulation of endogenous circadian rhythm in Arabidopsis. Previous studies
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Down-regulation of MANNANASE7 gene in Brassica napus L. enhances silique dehiscence-resistance Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-04 Yu-Long Li, Yan-Kun Yu, Ke-Ming Zhu, Li-Na Ding, Zheng Wang, Yan-Hua Yang, Jun Cao, Li-Zhang Xu, Yao-Ming Li, Xiao-Li Tan
Key message MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Abstract Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy yield loss in crops. The lack of dehiscence-resistant germplasm limits the
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Mitogen-activated protein kinase 11 (MAPK11) maintains growth and photosynthesis of potato plant under drought condition Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 Xi Zhu, Ning Zhang, Xue Liu, Shigui Li, Jiangwei Yang, Xusheng Hong, Fangfang Wang, Huaijun Si
Key message StMAPK11 overexpression promotes potato growth, physiological activities and photosynthesis under drought conditions. Abstract Mitogen-activated protein kinases (MAPKs) are import regulators of MAPK pathway in plants under drought condition. However, the critical role in potato (Solanum tuberosum L.) drought resistance is not fully understood. In this study, we aimed to explore the role
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A Rehmannia glutinosa cinnamate 4-hydroxylase promotes phenolic accumulation and enhances tolerance to oxidative stress Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 Yan Hui Yang, Heng Yang, Rui Fang Li, Cui Xiang Li, Lei Zeng, Chao Jie Wang, Na Li, Zhuang Luo
Key message RgC4H promotes phenolic accumulation in R. glutinosa, activating the molecular networks of its antioxidant systems, and enhancing the tolerance to oxidative stresses exposed to drought, salinity and H2O2 conditions. Abstract Rehmannia glutinosa is of great economic importance in China and increasing R. glutinosa productivity relies, in part, on understanding its tolerance to oxidative stress
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Skin colour, carotenogenesis and chlorophyll degradation mutant alleles: genetic orchestration behind the fruit colour variation in tomato Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 Tirthartha Chattopadhyay, Pranab Hazra, Shirin Akhtar, Deepak Maurya, Arnab Mukherjee, Sheuli Roy
The genetics underlying the fruit colour variation in tomato is an interesting area of both basic and applied research in plant biology. There are several factors, like phytohormones, environmental signals and epistatic interactions between genes, which modulate the ripe fruit colour in tomato. However, three aspects: genetic regulation of skin pigmentation, carotenoid biosynthesis and ripening-associated
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Three-dimensional imaging reveals that positions of cyst nematode feeding sites relative to xylem vessels differ between susceptible and resistant wheat Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 Kara A. Levin, Matthew R. Tucker, Christopher F. Strock, Jonathan P. Lynch, Diane E. Mather
Key message Resistance conferred by the Cre8 locus of wheat prevents cereal cyst nematode feeding sites from reaching and invading root metaxylem vessels. Abstract Cyst nematodes develop syncytial feeding sites within plant roots. The success of these sites is affected by host plant resistance. In wheat (Triticum aestivum L.), ‘Cre’ loci affect resistance against the cereal cyst nematode (CCN) Heterodera
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hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 Chakravarthi Mohan, Priscila Yumi Tanaka Shibao, Fernando Fonseca Pereira de Paula, Danyelle Toyama, Marcos Antonio Sanches Vieira, Antonio Figueira, Danielle Scotton, Andrea Soares-Costa, Flavio Henrique-Silva
Key message Transgenic sugarcane expressing V-ATPase subunit E dsRNA affects growth and survival of Sphenophorus levis. Abstract Plants being sessile organisms are constantly confronted with several biotic and abiotic stresses. Sugarcane (Saccharum spp) is a major tropical crop widely cultivated for its sugar and other by-products. In Brazil, sugarcane plantations account for significant production
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Genes and genome editing tools for breeding desirable phenotypes in ornamentals Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-03 A. Giovannini, M. Laura, B. Nesi, M. Savona, T. Cardi
Key message We review the main genes underlying commercial traits in cut flower species and critically discuss the possibility to apply genome editing approaches to produce novel variation and phenotypes. Abstract Promoting flowering and flower longevity as well as creating novelty in flower structure, colour range and fragrances are major objectives of ornamental plant breeding. The novel genome editing
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Isoflavone levels, nodulation and gene expression profiles of a CRISPR/Cas9 deletion mutant in the isoflavone synthase gene of red clover Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-02 Randy D. Dinkins, Julie Hancock, Brenda L. Coe, John B. May, Jack P. Goodman, William T. Bass, Jinge Liu, Yinglun Fan, Qiaolin Zheng, Hongyan Zhu
Key message Isoflavones are not involved in rhizobial signaling in red clover, but likely play a role in defense in the rhizosphere. Abstract Red clover (Trifolium pratense) is a high-quality forage legume, well suited for grazing and hay production in the temperate regions of the world. Like many legumes, red clover produces a number of phenylpropanoid compounds including anthocyanidins, flavan-3-ols
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Overexpressing a NPR1 -like gene from Citrus paradisi enhanced Huanglongbing resistance in C. sinensis Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-02 Aihong Peng, Xiuping Zou, Yongrui He, Shanchun Chen, Xiaofeng Liu, Jingyun Zhang, Qingwen Zhang, Zhu Xie, Junhong Long, Xiaochun Zhao
Key message Overexpression of CiNPR4 enhanced resistance of transgenic citrus plants to Huanglongbing by perceiving the salicylic acid and jasmonic acid signals and up-regulating the transcriptional activities of plant–pathogen interaction genes. Abstract Developing transgenic citrus plants with enhanced immunity is an efficient strategy to control citrus Huanglongbing (HLB). Here, a nonexpressor of
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ZEITLUPE enhances expression of PIF4 and YUC8 in the upper aerial parts of Arabidopsis seedlings to positively regulate hypocotyl elongation Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-02 Aya Saitoh, Tomoyuki Takase, Hiroshi Abe, Masaaki Watahiki, Yuki Hirakawa, Tomohiro Kiyosue
Key message Microarray and genetic analyses reveal that ZTL induces the expression of genes related to auxin synthesis, thereby promoting hypocotyl elongation. Abstract ZTL is a blue-light receptor that possesses a light–oxygen–voltage-sensing (LOV) domain, an F-box motif, and a kelch repeat domain. ZTL promotes hypocotyl elongation under high temperature (28 °C) in Arabidopsis thaliana; however, the
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Dynamics of cell wall structure and related genomic resources for drought tolerance in rice Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-02 Showkat Ahmad Ganie, Golam Jalal Ahammed
Key message Cell wall plasticity plays a very crucial role in vegetative and reproductive development of rice under drought and is a highly potential trait for improving rice yield under drought. Abstract Drought is a major constraint in rice (Oryza sativa L.) cultivation severely affecting all developmental stages, with the reproductive stage being the most sensitive. Rice plants employ multiple strategies
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FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana Plant Cell Rep. (IF 3.825) Pub Date : 2021-01-01 Hiroki Ayabe, Narumi Kawai, Mitsuhiro Shibamura, Yoichiro Fukao, Masaru Fujimoto, Nobuhiro Tsutsumi, Shin-ichi Arimura
Key message Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Abstract Organelle distribution is critical for effective metabolism and stress response and is controlled by various environmental factors. Clustered mitochondria (CLU) superfamily
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The ankyrin repeat-containing protein MdANK2B regulates salt tolerance and ABA sensitivity in Malus domestica Plant Cell Rep. (IF 3.825) Pub Date : 2020-12-17 Fu-jun Zhang, Yin-huan Xie, Han Jiang, Xun Wang, Yu-Jin Hao, Zhenlu Zhang, Chun-Xiang You
Key message The ankyrin repeat-containing protein MdANK2B was identified to contribute to increasing resistance to salt stress and decreasing sensitivity to ABA in Malus domestica. Abstract Ankyrin (ANK) repeat-containing proteins occur widely in prokaryotes, eukaryotes, and even in some viruses and play a critical role in plant growth and development, as well as the response to biotic and abiotic
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Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-29 Ali Raza
Key message Comprehensive metabolomic investigations provide a large set of stress-related metabolites and metabolic pathways, advancing crops under heat stress conditions. Metabolomics-assisted breeding, including mQTL and mGWAS boosted our understanding of improving numerous quantitative traits under heat stress. Abstract During the past decade, metabolomics has emerged as a fascinating scientific
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A functional molecular marker for detecting blister blight disease resistance in tea ( Camellia sinensis L.) Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-27 K. H. T. Karunarathna, K. M. Mewan, O. V. D. S. J. Weerasena, S. A. C. N. Perera, E. N. U. Edirisinghe
Key message Identification of an EST-SSR molecular marker associated with Blister blight, a common fungal disease of tea, facilitating marker-assisted selection, marking a milestone in tea molecular breeding. Abstract lister blight (BB) leaf disease of tea, caused by the fungus Exobasidium vexans, results in 25–30% crop loss annually. BB is presently controlled by Cu based fungicides, but genetic resistance
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Piriformospora indica colonization increases the growth, development, and herbivory resistance of sweet potato ( Ipomoea batatas L.) Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-24 Qing Li, Yun-Wei Kuo, Kuan-Hung Lin, Weiqun Huang, Caisheng Deng, Kai-Wun Yeh, Shi-Peng Chen
Key message Piriformospora indica symbiosis promoted the growth and photosynthesis, and simultaneously enhanced the resistance against insect herbivory by regulating sporamin-dependent defense in sweet potato. Abstract Piriformospora indica (P. indica), a versatile endophytic fungus, promotes the growth and confers resistance against multiple stresses by root colonization in plant hosts. In this study
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LhGST is an anthocyanin-related glutathione S-transferase gene in Asiatic hybrid lilies ( Lilium spp.) Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-19 Yuwei Cao, Leifeng Xu, Hua Xu, Panpan Yang, Guoren He, Yuchao Tang, Xianyu Qi, Meng Song, Jun Ming
Key message LhGST, an anthocyanin-related GST gene, was identified from Asiatic hybrid lilies. Expression and functional analyses demonstrated that LhGST might be involved in anthocyanin sequestration in lily tepals. Abstract Anthocyanins are responsible for the pink, red and purple pigmentation of flowers in Asiatic hybrid lilies, synthesized at the cytoplasmic surface of the endoplasmic reticulum
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ARF4 regulates shoot regeneration through coordination with ARF5 and IAA12 Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-12 Miao Miao Zhang, Huan Kai Zhang, Jun Feng Zhai, Xian Sheng Zhang, Ya Lin Sang, Zhi Juan Cheng
Key message ARF4-regulated shoot regeneration through competing with ARF5 for the interaction with IAA12. Abstract Plant possess the ability to regenerate shoot meristem and subsequent the whole individual. This process is the foundation for in vitro propagation and genetic engineering and provides a system for studying fundamental biological questions, such as hormonal signaling. Auxin response factor
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Seed-specific expression of TaYUC10 significantly increases auxin and protein content in wheat seeds Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-11 Yanlin Yang, Na Li, Wenrong Hui, Binjie Yuan, Pan Fan, Jingxia Liu, Honggang Wang, Deshun Feng
Key message Present study revealed that specific expression of TaYUC10.3 in wheat young seeds could increase the content of auxin, and protein. Abstract Auxin is a vital endogenous hormone in plants, which is involved in the regulation of various physiological and biochemical processes in plants. The flavin-containing monooxygenase encoded by the YUCCA gene is a rate-limiting enzyme in the tryptophan-dependent
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Impact of green synthesized WcAgNPs on in-vitro plant regeneration and withanolides production by inducing key biosynthetic genes in Withania coagulans Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-05 Deepika Tripathi, Krishna Kumar Rai, Shashi Pandey-Rai
Key message Withania coagulans (L.) Dunal bio-synthesized silver nanoparticles (WcAgNPs) worked as an abiotic elicitor or auto-catalyst that enhanced root regeneration and withanolides production in in-vitro regenerated W. coagulans. Abstract Rapid development in the production / consumption of silver nanoparticles (AgNPs) raised serious concern over its effects on the growth of natural plant community
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The caffeoyl-CoA O -methyltransferase gene SNP replacement in Russet Burbank potato variety enhances late blight resistance through cell wall reinforcement Plant Cell Rep. (IF 3.825) Pub Date : 2020-11-03 Niranjan Hegde, Sripad Joshi, Nancy Soni, Ajjamada C. Kushalappa
Key message Metabolic pathway gene editing in tetraploid potato enhanced resistance to late blight. Multiallelic mutation correction of a caffeoyl-CoA O-methyltransferase gene increased accumulation of resistance metabolites in Russet Burbank potato. Abstract Late blight of potato is a devastating disease worldwide and requires weekly applications of fungicides to manage. Genetic improvement is the
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A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways involved in CMS in kenaf Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-30 Meiqiong Tang, Zengqiang Li, Dengjie Luo, Fan Wei, Muhammad Haneef Kashif, Hai Lu, Yali Hu, Jiao Yue, Zhen Huang, Wenye Tan, Ru Li, Peng Chen
Key message Numbers of critical genes and pathways were found from the levels of transcriptome and metabolome, which were useful information for understanding of kenaf CMS mechanism. Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait in higher plants that leads to the inability to produce or release functional pollen. However, there is lack of comprehensive studies to reveal
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Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-24 Shijuan Liu, Changju Liu, Xue Wang, Huiqing Chen
Key message The promoter of the Arabidopsis thaliana β-glucosidase 19 gene directs GUS expression in a seed-specific manner in transgenic Arabidopsis and tobacco. Abstract In the present study, an 898-bp putative promoter of the Arabidopsis β-glucosidase 19 (AtBGLU19) gene was cloned. The bioinformatics analysis of the cis-acting elements indicated that this putative promoter contains many seed-specific
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A moso bamboo transcription factor, Phehdz1 , positively regulates the drought stress response of transgenic rice Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-24 Yameng Gao, Huanlong Liu, Kaimei Zhang, Fei Li, Min Wu, Yan Xiang
Key message 78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Abstract Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and development, signal transduction, and responses to environmental stresses. In
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IbERF71 , with IbMYB340 and IbbHLH2 , coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato ( Ipomoea batatas L.) Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-21 Zhiyuan Ning, Kangdi Hu, Zhilin Zhou, Donglan Zhao, Jun Tang, Hong Wang, Lixia Li, Chen Ding, Xiaoyan Chen, Gaifang Yao, Hua Zhang
Key message The transcription factor (TF) IbERF71 forms a novel complex, IbERF71-IbMYB340-IbbHLH2, to coregulate anthocyanin biosynthesis by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Abstract Purple-fleshed sweet potato (Ipomoea batatas L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating
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Somatic embryogenesis is an effective strategy for dissecting chimerism phenomena in Vitis vinifera cv Nebbiolo Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-21 Giorgio Gambino, Amedeo Moine, Paolo Boccacci, Irene Perrone, Chiara Pagliarani
Key Message The tendency of somatic embryogenesis to regenerate plants only from the L1 layer, associated with the spread of chimerism in grapevine, must be carefully considered in the framework of biotechnological improvement programmes. Abstract Grapevine is an important fruit crop with a high economic value linked to traditional genotypes that have been multiplied for centuries by vegetative propagation
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An OsNAM gene plays important role in root rhizobacteria interaction in transgenic Arabidopsis through abiotic stress and phytohormone crosstalk Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-21 Shalini Tiwari, Sateesh Chandra Gupta, Puneet Singh Chauhan, Charu Lata
Key message Overexpression of Bacillus amyloliquefaciens SN13-responsive OsNAM gene in Arabidopsis reveals its important role in beneficial plant and plant growth promoting rhizobacteria interaction by conferring stress tolerance and phytohormone modulation. Abstract Salinity is one of the major constraints that affect crop development and yield. Plants respond and adapt to salt stress via complex
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Fruit quality and DNA methylation are affected by parental order in reciprocal crosses of tomato Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-20 Magalí Diana Gimenez, Dana Valeria Vazquez, Felipe Trepat, Vladimir Cambiaso, Gustavo Rubén Rodríguez
Key message Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Abstract Crosses between different genotypes and even between different species are commonly used in plant breeding programs. Reciprocal hybrids are obtained by changing the cross direction
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Physiological and metabolic bases of increased growth in the tomato ethylene-insensitive mutant Never ripe : extending ethylene signaling functions Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-19 Vitor L. Nascimento, Auderlan M. Pereira, Aurelio S. Pereira, Victor F. Silva, Lucas C. Costa, Carla E. A. Bastos, Dimas M. Ribeiro, Camila Caldana, Ronan Sulpice, Adriano Nunes-Nesi, Agustin Zsögön, Wagner L. Araújo
Key message The tomato mutant Never ripe ( Nr ), a loss-of-function for the ethylene receptor Sl ETR3, shows enhanced growth, associated with increased carbon assimilation and a rewiring of the central metabolism. Abstract Compelling evidence has demonstrated the importance of ethylene during tomato fruit development, yet its role on leaf central metabolism and plant growth remains elusive. Here,
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CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-19 Mil Thi Tran, Duong Thi Hai Doan, Jihae Kim, Young Jong Song, Yeon Woo Sung, Swati Das, Eun‐Jung Kim, Geon Hui Son, Sang Hee Kim, Tien Van Vu, Jae-Yean Kim
Key message CRISPR/Cas9-based multiplexed editing of SlHyPRP1 resulted in precise deletions of its functional motif(s), thereby resulting in salt stress-tolerant events in cultivated tomato. Abstract Crop genetic improvement to address environmental stresses for sustainable food production has been in high demand, especially given the current situation of global climate changes and reduction of the
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Hevea brasiliensis coniferaldehyde-5-hydroxylase ( HbCAld5H ) regulates xylogenesis, structure and lignin chemistry of xylem cell wall in Nicotiana tabacum Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-17 S. Pramod, Thakurdas Saha, K. Rekha, P. B. Kavi Kishor
Key message The HbCAld5H1 gene cloned from Hevea brasiliensis regulates the cambial activity, xylem differentiation, syringyl–guaiacyl ratio, secondary wall structure, lignification pattern and xylan distribution in xylem fibres of transgenic tobacco plants. Abstract Molecular characterization of lignin biosynthesis gene coniferaldehyde-5-hydroxylase (CAld5H) from Hevea brasiliensis and its functional
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Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-17 Anabella Varela, Verónica N. Ibañez, Rodrigo Alonso, Diego Zavallo, Sebastián Asurmendi, Sebastián Gomez Talquenca, Carlos F. Marfil, Federico J. Berli
Key message By studying three cv. Malbec clones cultivated in two vineyards with contrasting environmental conditions, we demonstrated that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent. Abstract Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe
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NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance in Nicotiana attenuata Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-13 Min Yin, Na Song, Suiyun Chen, Jinsong Wu
Key message Here, we reported that a pathogen- and herbivore-induced Kunitz trypsin inhibitor gene, NaKTI2, is required for herbivore resistance, and transcriptionally regulated mainly by NaWRKY3 and NaWRKY6 but not Jasmonate signaling. Abstract Plant protease inhibitor (PI) occurs widely in plant species, and is considered as an important part of plant defense arsenal against herbivores. Transcriptome
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Dynamic cytokinin signaling and function of auxin in cytokinin responsive domains during rice crown root development Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-12 Ananya Neogy, Zeenu Singh, Khrang Khrang Khunggur Mushahary, Shri Ram Yadav
Key message We reveal the onset and dynamic tissue-specific cytokinin signaling domains and functional importance of auxin in the auxin–cytokinin interaction domains in shaping root architecture in the economically important rice plant. Abstract Plant hormones such as auxin and cytokinin are central regulators of root organogenesis. Typical in the grass species, the root system in rice is primarily
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In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long-term salt stress Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-10 Büşra Yazıcılar, Fatma Böke, Azize Alaylı, Hayrunisa Nadaroglu, Semin Gedikli, Ismail Bezirganoglu
Key message Ca2+ NPs enhanced tolerance of Triticale callus under salt stress by improving biochemical activity and confocal laser scanning analysis, conferring salt tolerance on callus cells. Abstract CaO NPs (Ca2+) are significant components that act as transducers in many adaptive and developmental processes in plants. In this study, effect of Ca2+ NPs on the response and regulation of the protective
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Jasmonic acid: a key frontier in conferring abiotic stress tolerance in plants Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-09 Ali Raza, Sidra Charagh, Zainab Zahid, Muhammad Salman Mubarik, Rida Javed, Manzer H. Siddiqui, Mirza Hasanuzzaman
Abiotic stresses are the primary sources of crop losses globally. The identification of key mechanisms deployed and established by plants in response to abiotic stresses is necessary for the maintenance of their growth and persistence. Recent discoveries have revealed that phytohormones or plant growth regulators (PGRs), mainly jasmonic acid (JA), have increased our knowledge of hormonal signaling
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Cytokinins as central regulators during plant growth and stress response Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-06 Si-Min Li, Hong-Xiang Zheng, Xian-Sheng Zhang, Na Sui
Key message Cytokinins are a class of phytohormone that participate in the regulation of the plant growth, development, and stress response. In this review, the potential regulating mechanism during plant growth and stress response are discussed. Abstract Cytokinins are a class of phytohormone that participate in the regulation of plant growth, physiological activities, and yield. Cytokinins also play
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CO 2 is a key constituent of the plant growth-promoting volatiles generated by bacteria in a sealed system Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-03 Chunlei Zhang, Mengyue Zhang, Zhenwei Yan, Fengxia Wang, Xianzheng Yuan, Shan Zhao, Lei Zhang, Huiyu Tian, Zhaojun Ding
Key message Plant growth is greatly inhibited in tightly sealed Petri dishes for lack of CO 2 . Bacteria which co-cultured with plant can produce CO 2 to promote plant growth in sealed systems. Abstract Bacteria produce a wide variety of volatiles, some of which can support and others can damage plant growth. It is a controversial issue whether CO2 or other bacterial volatile compounds promote plant
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Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-02 Konstantin Kanofsky, Jendrik Rusche, Lea Eilert, Fabian Machens, Reinhard Hehl
Key message WRKY50 from A. thaliana requires WT-boxes at target gene promoters for activation and binding. Abstract Based on the genome-wide prediction of WRKY50 target genes and the similarity of a WRKY50 binding site to WT-boxes in microbe-associated molecular pattern (MAMP)-responsive cis-regulatory modules (CRM), four WT-box containing CRMs from the promoter region of three WRKY50 target genes
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ScAOC1 , an allene oxide cyclase gene , confers defense response to biotic and abiotic stresses in sugarcane Plant Cell Rep. (IF 3.825) Pub Date : 2020-10-01 Tingting Sun, Guangli Cen, Chuihuai You, Wenyue Lou, Zhoutao Wang, Weihua Su, Wenju Wang, Damei Li, Youxiong Que, Yachun Su
Key message An allene oxide cyclase gene which is involved in defense against biotic and abiotic stresses was cloned and characterized in sugarcane. Abstract Allene oxide cyclase (AOC), a key enzyme in jasmonate acid (JA) biosynthesis, affects the stereoisomerism and biological activity of JA molecules, and plays an important role in plant stress resistance. In this study, four SsAOC alleles (SsAOC1–SsAOC4)
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Ectopic expression of nucleolar DEAD-Box RNA helicase OsTOGR1 confers improved heat stress tolerance in transgenic Chinese cabbage Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-29 Rajesh Yarra, Yongbiao Xue
Key message The DEAD-Box RNA helicase OsTOGR1 positively regulates heat stress tolerance in Chinese cabbage. Abstract Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is primarily cultivated vegetable crop in Asian countries. Heat stress is one of the major threats for its growth and yield. Numerous regulatory genes in various crops have shown to contribute thermotolerance. Among them
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Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-29 Mengyu Liu, Zhen Zhang, Zhixuan Xu, Lina Wang, Chunhua Chen, Zhonghai Ren
Key message SlMYB75 increased the accumulation of JA and improved the scavenging of excess H 2 O 2 to resist B. cinerea. Overexpression of SlMYB75 greatly prolongs tomato fruit storage life. Abstract Botrytis cinerea (B. cinerea) is a major threat to the production and storage life of tomato (Solanum lycopersicum) fruit around the world. SlMYB75 is an R2R3MYB transcription factor associated with the
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Transcriptomic and physiological analysis of OsCAO1 knockout lines using the CRISPR/Cas9 system in rice Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-27 Yu Jin Jung, Hyo Ju Lee, Jihyeon Yu, Sangsu Bae, Yong-Gu Cho, Kwon Kyoo Kang
Key message The altered rice leaf color based on the knockout of CAO1 gene generated using CRISPR/Cas9 technology plays important roles in chlorophyll degradation and ROS scavenging to regulate both natural and induced senescence in rice. Abstract Rice chlorophyllide a oxygenase (OsCAO1), identified as the chlorophyll b synthesis under light condition, plays a critical role in regulating rice plant
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OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-26 Yi Shi, Yan-Li Chang, Hai-Tao Wu, Abdullah Shalmani, Wen-Ting Liu, Wen-Qiang Li, Jian-Wei Xu, Kun-Ming Chen
Key message We found that a rice NADPH oxidase gene OsRbohB contributes drought tolerance and its functions are involved in the interaction of the OsRbohB-mediated ROS production and ABA signaling. Abstract The plasma membrane NADPH oxidases, also known as respiratory burst oxidase homologs, are the key producers of ROS under both normal and stress conditions in plants. However, their functions in
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Chromosome doubling methods in doubled haploid and haploid inducer-mediated genome-editing systems in major crops Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-25 Isidre Hooghvorst, Salvador Nogués
The doubled haploid technique aims to generate pure inbred lines for basic research and as commercial cultivars. The doubled haploid technique first generates haploid plants and is followed by chromosome doubling, which can be separated in time or overlapped, depending the procedure for each species. For a long time, much effort has been focused on haploid production via androgenesis, gynogenesis,
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Recent advances in plant thermomemory Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-25 Anand Nishad, Ashis Kumar Nandi
Key message This review summarizes the process of thermal acquired tolerance in plants and the knowledge gap compared to systemic acquired resistance that a plant shows after pathogen inoculation. Abstract Plants are continuously challenged by several biotic stresses such as pests and pathogens, or abiotic stresses like high light, UV radiation, drought, salt, and very high or low temperature. Interestingly
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The AtMYB2 inhibits the formation of axillary meristem in Arabidopsis by repressing RAX1 gene under environmental stresses. Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-24 Tianqi Jia,Kaidian Zhang,Fan Li,Yifeng Huang,Manman Fan,Tao Huang
Key message AtMYB2 protein represses the formation of axillary meristems in response to environmental stresses so that plants can undergo a shorter vegetative development stage under environmental stresses. Abstract Shoot branching is an important event determined by endogenous factors during the development of plants. The formation of axillary meristem is also significantly repressed by environmental
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Auxins, the hidden player in chloroplast development. Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-22 Alexis Salazar-Iribe,Clelia De-la-Peña
Throughout decades of plant research, the plant hormones known as auxins have been found to be of vital importance in most plant development processes. Indole-3-acetic acid (IAA) represents the most common auxin in plants and can be synthesized from its tryptophan precursor, which is synthesized in the chloroplast. The chloroplast constitutes an organelle of great relevance to plants since the photosynthesis
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Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endo- to ecodormancy in apple buds. Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-21 Amanda Malvessi Cattani,Vítor da Silveira Falavigna,Carolina Pereira Silveira,Vanessa Buffon,Felipe Dos Santos Maraschin,Giancarlo Pasquali,Luís Fernando Revers
Key Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The final step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this
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Simultaneous targeting of duplicated genes in Petunia protoplasts for flower color modification via CRISPR-Cas9 ribonucleoproteins. Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-21 Jihyeon Yu,Luhua Tu,Saminathan Subburaj,Sangsu Bae,Geung-Joo Lee
Key message We obtained a complete mutant line of Petunia having mutations in both F3H genes via Cas9-ribonucleoproteins delivery, which exhibited a pale purplish pink flower color. The CRISPR-Cas system is now revolutionizing agriculture by allowing researchers to generate various desired mutations in plants at will. In particular, DNA-free genome editing via Cas9-ribonucleoproteins (RNPs) delivery
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tRNA ADENOSINE DEAMINASE 3 is required for telomere maintenance in Arabidopsis thaliana. Plant Cell Rep. (IF 3.825) Pub Date : 2020-09-21 Sreyashree Bose,Ana Victoria Suescún,Jiarui Song,Claudia Castillo-González,Behailu Birhanu Aklilu,Erica Branham,Ryan Lynch,Dorothy E Shippen
Key message tRNA Adenosine Deaminase 3 helps to sustain telomere tracts in a telomerase-independent fashion, likely through regulating cellular metabolism. Abstract Telomere length maintenance is influenced by a complex web of chromatin and metabolism-related factors. We previously reported that a lncRNA termed AtTER2 regulates telomerase activity in Arabidopsis thaliana in response to DNA damage.