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  • In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants
    Plant Cell Rep. (IF 3.499) Pub Date : 2020-01-23
    Behzad Ahmadi, Hamed Ebrahimzadeh

    Abstract Androgenesis has become the most frequently chosen method of doubled haploid (DH) production in major crops. Theoretically, plantlets derived from in vitro cultured microspore encompass half of the normal chromosome number of donor plants and thus, considered to be haploid. However, depending on species/genotype and the method of haploid production, either via anther or isolated microspore culture, different ratios of spontaneous DHs and diploid (2n) or even polyploid plants originating from somatic tissues or unreduced gametes may also arise in the cultures. Adopting the method of haploid identification, anti-microtubular agent for restoring fertility, and discriminating spontaneous DHs from undesired heterozygote plants will substantially affect the success of androgenesis in breeding programs. The recent advances in the last 2 decades have made it possible to characterize the in vitro regenerants efficiently either prior to genome duplication or using in breeding programs. The herein described approaches and antimicotubular agents are, therefore, expected to improve the efficiency of DH-based breeding pipeline through the in vitro androgenesis.

    更新日期:2020-01-23
  • An efficient Agrobacterium -mediated genetic transformation method for foxtail millet ( Setaria italica L.)
    Plant Cell Rep. (IF 3.499) Pub Date : 2020-01-14
    Priyanka Sood, Roshan Kumar Singh, Manoj Prasad

    A simple and robust Agrobacterium-mediated gene expression system in the C4 panicoid model crop, foxtail millet has been developed with up to 27 % transformation efficiency.

    更新日期:2020-01-15
  • Root exudates: from plant to rhizosphere and beyond
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-07-25
    Vicente Vives-Peris, Carlos de Ollas, Aurelio Gómez-Cadenas, Rosa María Pérez-Clemente

    Abstract Key message This article describes the composition of root exudates, how these metabolites are released to the rhizosphere and their importance in the recruitment of beneficial microbiota that alleviate plant stress. Abstract Metabolites secreted to the rhizosphere by roots are involved in several processes. By modulating the composition of the root exudates, plants can modify soil properties to adapt and ensure their survival under adverse conditions. They use several strategies such as (1) changing soil pH to solubilize nutrients into assimilable forms, (2) chelating toxic compounds, (3) attracting beneficial microbiota, or (4) releasing toxic substances for pathogens, etc. In this work, the composition of root exudates as well as the different mechanisms of root exudation have been reviewed. Existing methodologies to collect root exudates, indicating their advantages and disadvantages, are also described. Factors affecting root exudation have been exposed, including physical, chemical, and biological agents which can produce qualitative and quantitative changes in exudate composition. Finally, since root exudates play an important role in the recruitment of mycorrhizal fungi and plant growth-promoting rhizobacteria (PGPR), the mechanisms of interaction between plants and the beneficial microbiota have been highlighted.

    更新日期:2020-01-14
  • An improved protocol for efficient transformation and regeneration of Setaria italica
    Plant Cell Rep. (IF 3.499) Pub Date : 2020-01-08
    C. M. Santos, D. Romeiro, J. P. Silva, M. F. Basso, H. B. C. Molinari, D. C. Centeno

    An efficient and improved transformation method for functional genetics studies in S. italica, being a boon for the Setaria scientific community and for crop improvement.

    更新日期:2020-01-09
  • APETALA 2 transcription factor CBX1 is a regulator of mycorrhizal symbiosis and growth of Lotus japonicus
    Plant Cell Rep. (IF 3.499) Pub Date : 2020-01-04
    Fang Liu, Yunjian Xu, Hequn Wang, Yuan Zhou, Beijiu Cheng, Xiaoyu Li

    Abstract Key message An AP2 family gene CBX1 is involved in mycorrhizal symbiosis and growth of Lotus japonicus. Abstract APETALA 2 (AP2) transcriptional regulator is highly conserved in plants. CBX1 from Lotus japonicus is a member of AP2 family. AMF (Arbuscular mycorrhizal fungi) inoculation experiment demonstrated that expression of CBX1 was significantly induced by AMF. Further promoter analysis showed that the – 764 to − 498 bp region of the CBX1 promoter containing CTTC motif is the AMF responsive region. Functional analysis of cbx1 mutant suggested CBX1 is critical for mycorrhizal symbiosis, especially for arbuscule formation. Moreover, under noncolonized condition, overexpression of CBX1 reduced the root length of L. japonicus but increased the size of root system and shoot length, whereas cbx1 mutant reduced the root size and shoot length, but not effect on root length. In addition, cbx1 altered activity of monolignol biosynthetic gene and increased lignin levels. Collectively, these data indicated that CBX1 is a positive regulator of symbiotic activity and plays roles in the growth of L. japonicus.

    更新日期:2020-01-07
  • The overexpression of OsSRO1a , which encodes an OsNINJA1- and OsMYC2-interacting protein, negatively affects OsMYC2-mediated jasmonate signaling in rice
    Plant Cell Rep. (IF 3.499) Pub Date : 2020-01-03
    Keita Kashihara, Tomonori Onohata, Rina Yariuchi, Suzumi Tanaka, Kazuya Akimitsu, Kenji Gomi

    Key message OsNINJA1-interacting protein, OsSRO1a, acts as a mediator that suppresses OsMYC2 activity in response to JA. Abstract Jasmonic acid (JA) is an important plant hormone for the stable growth and development of higher plants. The rice gene NOVEL INTERACTOR OF JAZ1 (OsNINJA1) interacts with Jasmonate ZIM-domain (JAZ) proteins and is a repressor of JA signaling. In this study, we identified several OsNINJA1-interacting proteins in rice from a yeast two-hybrid screen. Among the newly identified genes, we focused on SIMILAR TO RCD ONE1a (OsSRO1a) and investigated its role in JA signaling. Full-length OsSRO1a interacted with OsNINJA1 in plant cells but not in yeast cells. OsSRO1a also interacted with OsMYC2, a positive transcription factor in JA signaling, in both plant and yeast cells. The expression of OsSRO1a was upregulated at a late phase after JA treatment. Transgenic rice plants overexpressing OsSRO1a exhibited JA-insensitive phenotypes. In wild-type plants, JA induces resistance against rice bacterial blight, but this phenotype was suppressed in the OsSRO1a-overexpressing plants. Furthermore, the degradation of chlorophyll under dark-induced senescence conditions and the JA-induced upregulation of OsMYC2-responsive genes were suppressed in the OsSRO1a-overexpressing plants. These results suggest that OsSRO1a is a negative regulator of the OsMYC2-mediated JA signaling pathway in rice.

    更新日期:2020-01-04
  • Cytokinin oxidase PpCKX1 plays regulatory roles in development and enhances dehydration and salt tolerance in Physcomitrella patens
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-20
    Sujin Hyoung, Sung Hyun Cho, Joo Hee Chung, Won Mi So, Mei Hua Cui, Jeong Sheop Shin

    Abstract Key message PpCKX1 localizes to vacuoles and is dominantly expressed in the stem cells. PpCKX1 regulates developmental changes with increased growth of the rhizoid and enhances dehydration and salt tolerance. Abstract Cytokinins (CKs) are plant hormones that regulate plant development as well as many physiological processes, such as cell division, leaf senescence, control of shoot/root ratio, and reproductive competence. Cytokinin oxidases/dehydrogenases (CKXs) control CK concentrations by degradation, and thereby influence plant growth and development. In the moss Physcomitrella patens, an evolutionarily early divergent plant, we identified six putative CKXs that, by phylogenetic analysis, form a monophyletic clade. We also observed that ProPpCKX1:GUS is expressed specifically in the stem cells and surrounding cells and that CKX1 localizes to vacuoles, as indicated by Pro35S:PpCKX1-smGFP. Under normal growth conditions, overexpression of PpCKX1 caused many phenotypic changes at different developmental stages, and we suspected that increased growth of the rhizoid could affect those changes. In addition, we present evidence that the PpCKX1-overexpressor plants show enhanced dehydration and salt stress tolerance. Taken together, we suggest that PpCKX1 plays regulatory roles in development and adaptation to abiotic stresses in this evolutionarily early land plant species.

    更新日期:2019-12-20
  • Identification and functional characterization of squalene epoxidases and oxidosqualene cyclases from Tripterygium wilfordii
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-14
    Yuan Liu, Jiawei Zhou, Tianyuan Hu, Yun Lu, Linhui Gao, Lichan Tu, Jie Gao, Luqi Huang, Wei Gao

    We cloned two squalene epoxidases and five oxidosqualene cyclases, and identified their function using CRISPR/Cas9 tool and yeast heterologous expression.

    更新日期:2019-12-17
  • A novel maize dwarf mutant generated by Ty1 - copia LTR-retrotransposon insertion in Brachytic2 after spaceflight
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-13
    Chuan Li, Jin Tang, Zhaoyong Hu, Jingwen Wang, Tao Yu, Hongyang Yi, Moju Cao

    Retrotransposon insertion in Brachytic2 generated a new incomplete recessive dwarf allele after spaceflight can moderately reduce plant height in heterozygous and potentially improve maize yield.

    更新日期:2019-12-13
  • An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-11
    Jin Zhang, Jun Chen, Lijuan Wang, Shutang Zhao, Weina Wang, Jianbo Li, Bobin Liu, Xingyun Qi, Huanquan Zheng, Mengzhu Lu

    Trafficking protein particle (TRAPP) complexes subunit gene AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis.

    更新日期:2019-12-11
  • Glutamate dehydrogenase mediated amino acid metabolism after ammonium uptake enhances rice growth under aeration condition
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-09
    Cao Xiaochuang, Wu Meiyan, Zhu Chunquan, Zhong Chu, Zhang Junhua, Zhu Lianfeng, Wu Lianghuan, Jin Qianyu

    Aeration stimulates the rice growth and nitrogen (N) metabolism; in which, the glutamate accumulation limited by the glutamate dehydrogenase pathway after ammonia uptake may control root N metabolism during aeration.

    更新日期:2019-12-09
  • A gain-of-function mutation in Brassinosteroid-insensitive 2 alters Arabidopsis floral organ development by altering auxin levels
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-09
    Taotao Li, Xinke Kang, Lin Wei, Dawei Zhang, Honghui Lin

    Auxin can alter the fertility of bin2-1 plants and depends on the expression of SHY2.

    更新日期:2019-12-09
  • Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-12-03
    Shijing Xu, Yiqiu Zhang, Shengchun Li, Ling Chang, Yuyong Wu, Jiang Zhang

    The Bacillus thuringiensis (Bt) cry3Bb gene was successfully introduced into poplar plastid genome, leading to transplastomic poplar with high mortality to Plagiodera versicolora.

    更新日期:2019-12-03
  • Correction to: Improved insect resistance against Spodoptera litura in transgenic sweetpotato by overexpressing Cry1Aa toxin
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-29
    Yingying Zhong, Sulaiman Ahmed, Gaifang Deng, Weijuan Fan, Peng Zhang, Hongxia Wang

    In Materials and method section, a sweetpotato variety “Taizhong-6” (China national number 2013003) should be renamed as Ayamurasaki”.

    更新日期:2019-11-30
  • Four HD-ZIPs are involved in banana fruit ripening by activating the transcription of ethylene biosynthetic and cell wall-modifying genes
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-29
    Ying-Ying Yang, Wei Shan, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu

    Four MaHDZs are possibly involved in banana fruit ripening by activating the transcription of genes related to ethylene biosynthesis and cell wall degradation, such as MaACO5, MaEXP2, MaEXPA10, MaPG4 and MaPL4.

    更新日期:2019-11-30
  • Male and female inheritance patterns in tetraploid ‘Moncada’ mandarin
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-28
    Miguel Garavello, José Cuenca, Andrés Garcia-Lor, Neus Ortega, Luis Navarro, Patrick Ollitrault, Pablo Aleza

    Tetraploid `Moncada´ mandarin, used as male and female in interploidy hybridizations, displays mainly tetrasomic inheritance for most LGs, with slight variations according to the direction of the crossing.

    更新日期:2019-11-29
  • The novel protein CSAP accelerates leaf senescence and is negatively regulated by SAUL1 in the dark
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-26
    Won Mi So, Soo Youn Kim, Sujin Hyoung, Jeong Sheop Shin

    The chloroplast-localized protein CSAP is an ABA-responsive factor and positively regulates dark-induced senescence. This phenomenon is controlled by SAUL1 in Arabidopsis.

    更新日期:2019-11-27
  • Will the EU stay out of step with science and the rest of the world on plant breeding innovation?
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-21
    Petra Jorasch

    Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated under biotech regulations that include a novel combination of genetic material following the Cartagena protocol. In contrast to this, the European Court of Justice interpreted the current EU biotech regulations in a way that these also apply to plants resulting from new mutagenesis breeding, even if these plants are indistinguishable from conventionally bred plants. This ruling created strong reactions and concerns stating that recent technical developments have made the EU GMO Directive no longer fit for purpose. The article describes ongoing policy developments on EU level that might result in an update of current regulations.

    更新日期:2019-11-21
  • ZmMYC2 exhibits diverse functions and enhances JA signaling in transgenic Arabidopsis
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-18
    Jingye Fu, Lijun Liu, Qin Liu, Qinqin Shen, Chang Wang, Panpan Yang, Chenying Zhu, Qiang Wang

    ZmMYC2 was identified as the key regulator of JA signaling in maize and exhibited diverse functions through binding to many gene promoters as well as enhanced JA signaling in transgenic Arabidopsis.

    更新日期:2019-11-19
  • Genetic modification in Malaysia and India: current regulatory framework and the special case of non-transformative RNAi in agriculture
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-07-26
    Jasdeep Kaur Darsan Singh, Nurzatil Sharleeza Mat Jalaluddin, Neeti Sanan-Mishra, Jennifer Ann Harikrishna

    Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived varieties of crops become more useful in the field, it is important to examine the capacity of current regulatory systems to deal with such varieties, and to determine if changes are needed to improve the existing frameworks. We review the biosafety frameworks from the perspective of developing countries that are increasingly involved in modern biotechnology research, including RNAi applications, and make some recommendations. Malaysia and India have approved laws regulating living modified organisms and products thereof, highlighting that the use of any genetically modified step requires regulatory scrutiny. In view of production methods for exogenously applied double-stranded RNAs and potential risks from the resulting double-stranded RNA-based products, we argue that a process-based system may be inappropriate for the non-transformative RNAi technology. We here propose that the current legislation needs rewording to take account of the non-transgenic RNAi technology, and discuss the best alternative for regulatory systems in India and Malaysia in comparison with the existing frameworks in other countries.

    更新日期:2019-11-18
  • Ectopic expression of LoSVP , a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-18
    Xiaoli Tang, Meixia Liang, Junjie Han, Jieshan Cheng, Hongxia Zhang, Xiaohua Liu

    A MADS-domain transcription factor LoSVP , which could delay flowering through vernalization pathway, was isolated from lily.

    更新日期:2019-11-18
  • Differential anthocyanin accumulation in radish taproot: importance of RsMYB1 gene structure
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-14
    Biao Lai, Yuanyi Cheng, Hong Liu, Qiuxia Wang, Qi Wang, Chunlan Wang, Rui Su, Fabo Chen, Huicong Wang, Lina Du

    RsMYB1a was the crucial MYB, and RsbHLH4 is the essential partner in regulating the anthocyanin biosynthesis in radish.

    更新日期:2019-11-14
  • Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-14
    Wusheng Liu, Mary R. Rudis, Matthew H. Cheplick, Reginald J. Millwood, Jian-Ping Yang, Christine A. Ondzighi-Assoume, Garrett A. Montgomery, Kellie P. Burris, Mitra Mazarei, Jonathan D. Chesnut, Charles Neal Stewart

    A novel and robust lipofection-mediated transfection approach for the use of DNA-free Cas9/gRNA RNP for gene editing has demonstrated efficacy in plant cells.

    更新日期:2019-11-14
  • ABA signaling is negatively regulated by GbWRKY1 through JAZ1 and ABI1 to affect salt and drought tolerance
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-12
    Xiangyin Luo, Chao Li, Xin He, Xianlong Zhang, Longfu Zhu

    GbWRKY1 can function as a negative regulator of ABA signaling via JAZ1 and ABI1, with effects on salt and drought tolerance.

    更新日期:2019-11-13
  • Benzoate-CoA ligase contributes to the biosynthesis of biphenyl phytoalexins in elicitor-treated pear cell cultures
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-12
    Shashank Sagar Saini, Mariam Gaid, Debabrata Sircar

    Benzoate-Coenzyme A ligase enzyme activity catalyzing the conversion of free benzoic acid to benzoyl-CoA was detected and biochemically characterized in the elicitor-treated pear cell cultures.

    更新日期:2019-11-13
  • Time-dependent behavior of phenylpropanoid pathway in response to methyl jasmonate in Scrophularia striata cell cultures
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-09
    Ehsan Sadeghnezhad, Mohsen Sharifi, Hassan Zare-Maivan, Najmeh Ahmadian Chashmi

    MeJA triggers a time-dependent behavior of the phenylpropanoid compounds.

    更新日期:2019-11-11
  • Drought-induced disturbance of carbohydrate metabolism in anthers and male abortion of two Gossypium hirsutum cultivars differing in drought tolerance
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-04
    Wei Hu, Yanjun Huang, Dimitra A. Loka, Hua Bai, Yu Liu, Shanshan Wang, Zhiguo Zhou

    Cotton pollen abortion, under drought stress, was closely associated with changes in anther carbohydrate metabolism, and pollen abortion rate due to drought was higher in drought-sensitive cultivars than drought-tolerant cultivars.

    更新日期:2019-11-04
  • The subcellular localization of two isopentenyl diphosphate isomerases in rice suggests a role for the endoplasmic reticulum in isoprenoid biosynthesis
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-11-02
    Xin Jin, Can Baysal, Lihong Gao, Vicente Medina, Margit Drapal, Xiuzhen Ni, Yanmin Sheng, Lianxuan Shi, Teresa Capell, Paul D. Fraser, Paul Christou, Changfu Zhu

    Both OsIPPI1 and OsIPPI2 enzymes are found in the endoplasmic reticulum, providing novel important insights into the role of this compartment in the synthesis of MVA pathway isoprenoids.

    更新日期:2019-11-04
  • Plastid biogenesis and homeostasis.
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-06-06
    Inhwan Hwang

    更新日期:2019-11-01
  • 更新日期:2019-11-01
  • Dendrobium micropropagation: a review.
    Plant Cell Rep. (IF 3.499) Pub Date : 2015-06-06
    Jaime A Teixeira da Silva,Jean Carlos Cardoso,Judit Dobránszki,Songjun Zeng

    Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation.

    更新日期:2019-11-01
  • The utility of green fluorescent protein in transgenic plants.
    Plant Cell Rep. (IF 3.499) Pub Date : 2002-11-27
    C N Stewart

    The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

    更新日期:2019-11-01
  • Specimen block counter-staining for localization of GUS expression in transgenic arabidopsis and tobacco.
    Plant Cell Rep. (IF 3.499) Pub Date : 2002-11-27
    M K Kim,J-W Choi,J-H Jeon,V R Franceschi,L B Davin,N G Lewis

    A simple counter-staining procedure has been developed for comparative beta-glucuronidase (GUS) expression and anatomical localization in transgenic herbaceous arabidopsis and tobacco. This protocol provides good anatomical visualization for monitoring chimeric gene expression at both the organ and tissue levels. It can be used with different histochemical stains and can be extended to the study of woody species. The specimens are paraffin-embedded, the block is trimmed to reveal internal structure, safranin-O staining solution is briefly applied to the surface of the block, then washed off and, after drying, a drop of immersion oil is placed on the stained surface for subsequent photographic work. This gives tissue counter-staining with good structural preservation without loss of GUS staining product; moreover, sample observation is rapid and efficient compared to existing procedures.

    更新日期:2019-11-01
  • Characterization of the yeast copper-inducible promoter system in Arabidopsis thaliana.
    Plant Cell Rep. (IF 3.499) Pub Date : 2002-05-25
    C L Granger,R J Cyr

    Inducible promoters or gene-switches are used to both spatially and temporally regulate gene expression. Such regulation can provide information concerning the function of a gene in a developmental context as well as avoid potential harmful effects due to overexpression. A gfp construct under the control of a copper-inducible promoter was introduced into Arabidopsis thaliana (L.) Heynh. and the regulatory parameters of this inducible promoter were determined. Here, we describe the time-course of up- and down-regulation of GFP expression in response to copper level, the optimal regulatory levels of copper, and the tissue specificity of expression in three transgenic lines. We conclude that the copper-inducible promoter system may be useful in regulating the time and location of gene expression in A. thaliana.

    更新日期:2019-11-01
  • Gravity and light control of the developmental polarity of regenerating protoplasts isolated from prothallial cells of the fern Ceratopteris richardii.
    Plant Cell Rep. (IF 3.499) Pub Date : 2001-09-07
    E S Edwards,S J Roux

    A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protoplasts by floating them up into a 0.5 M sorbitol layer. Regeneration occurred over a period of 10-24 days, and, under optimal osmotic conditions, followed the developmental pattern seen during spore germination, in that the first division gave rise to a primary rhizoid. Thus, prothallial protoplasts are comparable to germinating spores as suitable models for studies of developmental polarity in single cells. As in germinating spores, the polarity of development in regenerating protoplasts is influenced by the vectors of gravity and unilateral light. However, the relative influence of light in fixing this polarity is greater in regenerating protoplasts, while in germinating spores, the influence of gravity is greater.

    更新日期:2019-11-01
  • Comparative ultrastructure of fruit plastids in three genetically diverse genotypes of apple (Malus × domestica Borkh.) during development.
    Plant Cell Rep. (IF 3.499) Pub Date : 2017-07-13
    Scott M Schaeffer,Ryan Christian,Nohely Castro-Velasquez,Brennan Hyden,Valerie Lynch-Holm,Amit Dhingra

    Comparative ultrastructural developmental time-course analysis has identified discrete stages at which the fruit plastids undergo structural and consequently functional transitions to facilitate subsequent development-guided understanding of the complex plastid biology. Plastids are the defining organelle for a plant cell and are critical for myriad metabolic functions. The role of leaf plastid, chloroplast, is extensively documented; however, fruit plastids-chromoplasts-are poorly understood, especially in the context of the diverse metabolic processes operating in these diverse plant organs. Recently, in a comparative study of the predicted plastid-targeted proteomes across seven plant species, we reported that each plant species is predicted to harbor a unique set of plastid-targeted proteins. However, the temporal and developmental context of these processes remains unknown. In this study, an ultrastructural analysis approach was used to characterize fruit plastids in the epidermal and collenchymal cell layers at 11 developmental timepoints in three genotypes of apple (Malus × domestica Borkh.): chlorophyll-predominant 'Granny Smith', carotenoid-predominant 'Golden Delicious', and anthocyanin-predominant 'Top Red Delicious'. Plastids transitioned from a proplastid-like plastid to a chromoplast-like plastid in epidermis cells, while in the collenchyma cells, they transitioned from a chloroplast-like plastid to a chloro-chromo-amyloplast plastid. Plastids in the collenchyma cells of the three genotypes demonstrated a diverse array of structures and features. This study enabled the identification of discrete developmental stages during which specific functions are most likely being performed by the plastids as indicated by accumulation of plastoglobuli, starch granules, and other sub-organeller structures. Information regarding the metabolically active developmental stages is expected to facilitate biologically relevant omics studies to unravel the complex biochemistry of plastids in perennial non-model systems.

    更新日期:2019-11-01
  • Engineering gibberellin metabolism in Solanum nigrum L. by ectopic expression of gibberellin oxidase genes.
    Plant Cell Rep. (IF 3.499) Pub Date : 2012-01-13
    A Bhattacharya,D A Ward,P Hedden,A L Phillips,J B Power,M R Davey

    Gibberellins (GAs) control many aspects of plant development, including seed germination, shoot growth, flower induction and growth and fruit expansion. Leaf explants of Solanum nigrum (Black Nightshade; Solanaceae) were used for Agrobacterium-mediated delivery of GA-biosynthetic genes to determine the influence of their encoded enzymes on the production of bioactive GAs and plant stature in this species. Constructs were prepared containing the neomycin phosphotransferase (nptII) gene for kanamycin resistance as a selectable marker, and the GA-biosynthetic genes, their expression under the control of the CaMV 35S promoter. The GA-biosynthetic genes comprised AtGA20ox1, isolated from Arabidopsis thaliana, the product from which catalyses the formation of C(19)-GAs, and MmGA3ox1 and MmGA3ox2, isolated from Marah macrocarpus, which encode functionally different GA 3-oxidases that convert C(19)-GAs to biologically active forms. Increase in stature was observed in plants transformed with AtGA20ox1, MmGA3ox2 and MmGA3ox1 + MmGA3ox2, their presence and expression being confirmed by PCR and RT-PCR, respectively, accompanied by an increase in GA(1) content. Interestingly, MmGA3ox1 alone did not induce a sustained increase in plant height, probably because of only a marginal increase in bioactive GA(1) content in the transformed plants. The results are discussed in the context of regulating plant stature, since this strategy would decrease the use of chemicals to promote plant growth.

    更新日期:2019-11-01
  • Over-expression of a gibberellin 2-oxidase gene from Phaseolus coccineus L. enhances gibberellin inactivation and induces dwarfism in Solanum species.
    Plant Cell Rep. (IF 3.499) Pub Date : 2007-11-14
    C Dijkstra,E Adams,A Bhattacharya,A F Page,P Anthony,S Kourmpetli,J B Power,K C Lowe,S G Thomas,P Hedden,A L Phillips,M R Davey

    Gibberellins (GAs) are endogenous hormones that play a predominant role in regulating plant stature by increasing cell division and elongation in stem internodes. The product of the GA 2-oxidase gene from Phaseolus coccineus (PcGA2ox1) inactivates C(19)-GAs, including the bioactive GAs GA(1 )and GA(4), by 2beta-hydroxylation, reducing the availability of these GAs in plants. The PcGA2ox1 gene was introduced into Solanum melanocerasum and S. nigrum (Solanaceae) by Agrobacterium-mediated transformation with the aim of decreasing the amounts of bioactive GA in these plants and thereby reducing their stature. The transgenic plants exhibited a range of dwarf phenotypes associated with a severe reduction in the concentrations of the biologically active GA(1) and GA(4). Flowering and fruit development were unaffected. The transgenic plants contained greater concentrations of chlorophyll b (by 88%) and total chlorophyll (11%), although chlorophyll a and carotenoid contents were reduced by 8 and 50%, respectively. This approach may provide an alternative to the application of chemical growth retardants for reducing the stature of plants, particularly ornamentals, in view of concerns over the potential environmental and health hazards of such compounds.

    更新日期:2019-11-01
  • Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana.
    Plant Cell Rep. (IF 3.499) Pub Date : 2010-02-20
    Ho Yong Chung,Shozo Fujioka,Sunghwa Choe,Soyoun Lee,Youn Hyung Lee,Nam In Baek,In Sik Chung

    We generated transgenic lines of Arabidopsis thaliana with an RNA interference construct that expressed hairpin double-stranded RNA for DET2:DWF4:SMT2 to induce sequence-specific RNA silencing. In transgenic plants, expressions of DET2, DWF4, and SMT2 were simultaneously reduced, and the campesterol content was increased by up to 420% compared to the level in the wild-type plant. Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates. Transgenic plants harboring the RNA interference construct displayed a semi-dwarf phenotype due to altered development. Our findings indicate that redesigning of plant architecture is possible through simultaneous suppression of multiple genes involved in BR biosynthesis.

    更新日期:2019-11-01
  • Detectableβ-glucuronidase activity in transgenic cranberry is affected by endogenous inhibitors and plant development.
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-06-01
    R Serres,B McCown,E Zeldin

    Extracts of cranberry, rich in flavonols and proanthocyanins, inactivatedβ-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing thegusA gene. Histochemical GUS assays produced random and generally unpredictable staining. The addition of poly-vinylpolypyrrolidone (PVPP) during the preparation of transgenic cranberry leaf extracts increased the detectable GUS activity in fluorogenic assays more than 200-fold. Detectable GUS activity varied among transclones and also within a transclone depending on the developmental and physiological state of the tissue, as well as the growth environment. Use of PVPP altered the relative ranking of plants based on their total transgenic enzyme activity and resulted in different conclusions as to the effects of genotype or growth environment on transgene expression.

    更新日期:2019-11-01
  • Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica).
    Plant Cell Rep. (IF 3.499) Pub Date : 2013-01-15
    Yuan-Yuan Li,Ke Mao,Cheng Zhao,Xian-Yan Zhao,Rui-Fen Zhang,Hua-Lei Zhang,Huai-Rui Shu,Yu-Jin Hao

    MdCRY2 was isolated from apple fruit skin, and its function was analyzed in MdCRY2 transgenic Arabidopsis. The interaction between MdCRY2 and AtCOP1 was found by yeast two-hybrid and BiFC assays. Cryptochromes are blue/ultraviolet-A (UV-A) light receptors involved in regulating various aspects of plant growth and development. Investigations of the structure and functions of cryptochromes in plants have largely focused on Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), pea (Pisum sativum), and rice (Oryza sativa). However, no data on the function of CRY2 are available in woody plants. In this study, we isolated a cryptochrome gene, MdCRY2, from apple (Malus domestica). The deduced amino acid sequences of MdCRY2 contain the conserved N-terminal photolyase-related domain and the flavin adenine dinucleotide (FAD) binding domain, as well as the C-terminal DQXVP-acidic-STAES (DAS) domain. Relationship analysis indicates that MdCRY2 shows the highest similarity to the strawberry FvCRY protein. The expression of MdCRY2 is induced by blue/UV-A light, which represents a 48-h circadian rhythm. To investigate the function of MdCRY2, we overexpressed the MdCRY2 gene in a cry2 mutant and wild type (WT) Arabidopsis, assessed the phenotypes of the resulting transgenic plants, and found that MdCRY2 functions to regulate hypocotyl elongation, root growth, flower initiation, and anthocyanin accumulation. Furthermore, we examined the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. These data provide functional evidence for a role of blue/UV-A light-induced MdCRY2 in controlling photomorphogenesis in apple.

    更新日期:2019-11-01
  • Balanced activity of microRNA166/165 and its target transcripts from the class III homeodomain-leucine zipper family regulates root growth in Arabidopsis thaliana.
    Plant Cell Rep. (IF 3.499) Pub Date : 2014-02-08
    Archita Singh,Sharmila Singh,Kishore C S Panigrahi,Ralf Reski,Ananda K Sarkar

    Overexpression of miR166/165 down-regulates target HD - ZIP IIIs and promotes root growth by enhancing cell division and meristematic activity, whereas overexpression of HD - ZIP IIIs inhibits root growth in Arabidopsis thaliana. Post-embryonic growth of higher plants is maintained by active meristems harbouring undifferentiated cells. Shoot and root apical meristems (SAM and RAM) utilize both similar and distinct signalling mechanisms for their maintenance in Arabidopsis thaliana. An important regulatory role in this context has the interaction of microRNAs with their target mRNAs, mostly encoding transcription factors. One class of microRNA166/165 (miR166/165) has been implicated in the maintenance of SAM and vascular patterning. Here, we show that miR166/165 plays an important role in root growth also by negatively regulating its target transcripts, HD-ZIP IIIs, in the RAM. While overexpression of miR166 promotes RAM activity, overexpression of its targets reduces RAM activity. These results reveal a conserved role of miR166/165 in the maintenance of SAM and RAM activity in A. thaliana.

    更新日期:2019-11-01
  • A tobacco homolog of DCN1 is involved in pollen development and embryogenesis.
    Plant Cell Rep. (IF 3.499) Pub Date : 2014-04-22
    Julia Hosp,Alexandra Ribarits,Katarzyna Retzer,Yongfeng Jin,Alisher Tashpulatov,Tatiana Resch,Christina Friedmann,Elisabeth Ankele,Viktor Voronin,Klaus Palme,Erwin Heberle-Bors,Alisher Touraev

    We show that DCN1 binds ubiquitin and RUB/NEDD8, associates with cullin, and is functionally conserved. DCN1 activity is required for pollen development transitions and embryogenesis, and for pollen tube growth. Plant proteomes show remarkable plasticity in reaction to environmental challenges and during developmental transitions. Some of this adaptability comes from ubiquitin-mediated protein degradation regulated by cullin-RING E3 ubiquitin ligases (CRLs). CRLs are activated through modification of the cullin subunit with the ubiquitin-like protein RUB/NEDD8 by an E3 ligase called defective in cullin neddylation 1 (DCN1). Here we show that tobacco DCN1 binds ubiquitin and RUB/NEDD8 and associates with cullin. When knocked down by RNAi, tobacco pollen formation was affected and zygotic embryogenesis was blocked around the globular stage. Additionally, we found that RNAi of DCN1 inhibited the stress-triggered reprogramming of cultured microspores from their intrinsic gametophytic mode of development to an embryogenic state. This stress-induced developmental switch is a known feature in many important crops and leads ultimately to the formation of haploid embryos and plants. Compensating the RNAi effect by re-transformation with a promoter-silencing construct restored pollen development and zygotic embryogenesis, as well as the ability for stress-induced formation of embryogenic microspores. Overexpression of DCN1 accelerated pollen tube growth and increased the potential for microspore reprogramming. These results demonstrate that the biochemical function of DCN1 is conserved in plants and that its activity is involved in transitions during pollen development and embryogenesis, and for pollen tube growth.

    更新日期:2019-11-01
  • Precision genetic engineering tools for next-generation plant breeding.
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-03-30
    Günther Hahne,Laurence Tomlinson,Fabien Nogué

    更新日期:2019-11-01
  • Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato.
    Plant Cell Rep. (IF 3.499) Pub Date : 2019-01-17
    Benoit Danilo,Laura Perrot,Kostlend Mara,Emmanuel Botton,Fabien Nogué,Marianne Mazier

    更新日期:2019-11-01
  • Arabidopsis DAL1 and DAL2, two RING finger proteins homologous to Drosophila DIAP1, are involved in regulation of programmed cell death.
    Plant Cell Rep. (IF 3.499) Pub Date : 2010-10-26
    B M Vindhya S Basnayake,Dayong Li,Huijuan Zhang,Guojun Li,Nasar Virk,Fengming Song

    Programmed cell death (PCD) is a precise, genetically controlled cellular process with important roles in plant growth, development, and response to biotic and abiotic stress. However, the genetic mechanisms that control PCD in plants are unclear. Two Arabidopsis genes, DAL1 and DAL2 (for Drosophila DIAP1 like 1 and 2), encoding RING finger proteins with homology to DIAP1 were identified, and a series of experiments were performed to elucidate their roles in the regulation of PCD and disease resistance. Expression of DAL1 and DAL2 genes was induced in Arabidopsis plants after inoculation with virulent and avirulent strains of Pseudomonas syrinage pv. tomato (Pst) DC3000 or after infiltration with fumonisin B1 (FB1). Plants with mutations in the DAL1 and DAL2 genes displayed more severe disease after inoculation with an avirulent strain of Pst DC3000, but they showed similar disease severity as the wild-type plant after inoculation with a virulent strain of Pst DC3000. Significant accumulations of reactive oxygen species (ROS) and increased cell death were observed in the dal1 and dal2 mutant plants after inoculation with the avirulent strain of Pst DC3000. The dal mutant plants underwent extensive PCD upon infiltration of FB1 and displayed higher levels of ROS accumulation, callose deposition, and autofluorescence than the wild-type plants. Our data suggest that DAL1 and DAL2 may act as negative regulators of PCD in Arabidopsis.

    更新日期:2019-11-01
  • The plasma membrane-localised Ca(2+)-ATPase ACA8 plays a role in sucrose signalling involved in early seedling development in Arabidopsis.
    Plant Cell Rep. (IF 3.499) Pub Date : 2014-03-04
    Jie Zhang,Xudong Zhang,Ruiping Wang,Weiqi Li

    Arabidopsis Ca (2+) -ATPase ACA8 plays a role in sucrose signalling during early seedling development by integrating developmental signals with carbon source availability. Calcium (Ca(2+)) is an essential signal transduction element in eukaryotic organisms. Changes in the levels of intracellular Ca(2+) affect multiple developmental processes in plants, including cell division, polar growth, and organogenesis. Here, we report that the plasma-membrane-localised Arabidopsis Ca(2+)-ATPase ACA8 plays a role in sucrose signalling during early seedling development. Disruption of the ACA8 gene elevated the expression of genes that encode transporters for Ca(2+) efflux. The seedlings that carried a T-DNA insertion mutation in ACA8 experienced water stress during early development. This response was unrelated to inadequate osmoregulatory responses and was most likely caused by disruption of cell membrane integrity and severe ion leakage. In addition, aca8-1 seedlings displayed a significant decline in photosynthetic performance and arrested root growth after removal of sucrose from the growth medium. The two phenomena resulted from impaired photosynthesis, reduced cell proliferation in the root meristem and the sucrose control of cell-cycle events. All of the stress-response phenotypes were rescued when expression of ACA8 was restored in aca8-1 mutant. Taken together, our results indicate that ACA8-mediated Ca(2+) signalling contributes to modulate early seedling development and coordinates root development with nutrient availability.

    更新日期:2019-11-01
  • Arabidopsis ACA7, encoding a putative auto-regulated Ca(2+)-ATPase, is required for normal pollen development.
    Plant Cell Rep. (IF 3.499) Pub Date : 2011-11-03
    Noel Lucca,Gabriel León

    Microgametogenesis is a complex process that involves numerous well-coordinated cell activities, ending with the production of pollen grains. Pollen development has been studied at the cytological level in Arabidopsis and other plant species, where its temporal time course has been defined. However, the molecular mechanism underlying this process is still unclear, since a relative small number of genes and/or processes have been identified as essential for pollen development. We have designed a methodology to select candidate genes for functional analysis, based on transcriptomic data obtained from different stages of pollen development. From our analyses, we selected At2g22950 as a candidate gene; this gene encodes a protein belonging to the auto-regulated Ca(2+)-ATPase family, ACA7. Microarray data indicate that ACA7 is expressed exclusively in developing pollen grains, with the highest level of mRNA at the time of the second pollen mitosis. Our RT-PCR experiments showed that ACA7 mRNA is detected exclusively in developing flowers. Confocal microscopy experiments showed a plasma membrane localization for the recombinant GFP:ACA7 protein. We identified two different insertional mutant lines, aca7-1 and aca7-2; plants from both mutant lines displayed a normal vegetative development but showed large amounts of dead pollen grains in mature flowers assayed by Alexander's staining. Histological analysis indicated that abnormalities are detected after the first pollen mitosis and we found a strong correlation between ACA7 mRNA accumulation and the severity of the phenotype. Our results indicate that ACA7 is a plasma membrane protein that has an important role during pollen development, possibly through regulation of Ca(2+) homeostasis.

    更新日期:2019-11-01
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  • Erratum to: Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.
    Plant Cell Rep. (IF 3.499) Pub Date : 2016-12-22
    Fátima Duarte-Aké,Eduardo Castillo-Castro,Felipe Barredo Pool,Francisco Espadas,Jorge M Santamaría,Manuel L Robert,Clelia De-la-Peña

    更新日期:2019-11-01
  • Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes.
    Plant Cell Rep. (IF 3.499) Pub Date : 2007-07-20
    Takashi Nakatsuka,Yoshiko Abe,Yuko Kakizaki,Saburo Yamamura,Masahiro Nishihara

    Orange- to red-colored flowers are difficult to produce by conventional breeding techniques in some floricultural plants. This is due to the deficiency in the formation of pelargonidin, which confers orange to red colors, in their flowers. Previous researchers have reported that brick-red colored flowers can be produced by introducing a foreign dihydroflavonol 4-reductase (DFR) with different substrate specificity in Petunia hybrida, which does not accumulate pelargonidin pigments naturally. However, because these experiments used dihydrokaempferol (DHK)-accumulated mutants as transformation hosts, this strategy cannot be applied directly to other floricultural plants. Thus in this study, we attempted to produce red-flowered plants by suppressing two endogenous genes and expressing one foreign gene using tobacco as a model plant. We used a chimeric RNAi construct for suppression of two genes (flavonol synthase [FLS] and flavonoid 3'-hydroxylase [F3'H]) and expression of the gerbera DFR gene in order to accumulate pelargonidin pigments in tobacco flowers. We successfully produced red-flowered tobacco plants containing high amounts of additional pelargonidin as confirmed by HPLC analysis. The flavonol content was reduced in the transgenic plants as expected, although complete inhibition was not achieved. Expression analysis also showed that reduction of the two-targeted genes and expression of the foreign gene occurred simultaneously. These results demonstrate that flower color modification can be achieved by multiple gene regulation without use of mutants if the vector constructs are designed resourcefully.

    更新日期:2019-11-01
  • Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing.
    Plant Cell Rep. (IF 3.499) Pub Date : 2018-05-26
    Shouwei Tian,Linjian Jiang,Xiaxia Cui,Jie Zhang,Shaogui Guo,Maoying Li,Haiying Zhang,Yi Ren,Guoyi Gong,Mei Zong,Fan Liu,Qijun Chen,Yong Xu

    更新日期:2019-11-01
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  • Gemmation in cultured gametophytes ofOsmunda regalis.
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-02-01
    Helena Fernández,Ana María Bertrand,Ricardo Sánchez-Tamés

    Gametophytes ofOsmunda regalis were culturedin vitro to determine the optimal conditions for growth and development. Culture media with low ammonium concentration (Knop, Knudson or 1/4 Murashige and Skoog dilution) were the most effective for growth of this organism. Addition of sucrose and mannitol to the culture medium at the same osmoticum, inhibits or enhances, respectively, growth and development of the gametophytes, which show a strong autotrophyin vitro. In darkness, both two-dimensional growth and sexual organ formation took place. Gemmae formation is described for the first time in this species and this process does not occur without sucrose in the culture medium or in darkness.

    更新日期:2019-11-01
  • Somatic embryogenesis inGnetum ula Brongn. (Gnetum edule) (Willd) Blume.
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-02-01
    Alice Clara Augustine,L D'Souza

    Somatic embryos ofGnetum ula (Gnetum edule) an endangered gymnosperm closely related to the angiosperms have been induced in vitro. Megagametophyte tissue with immature embryos was cultured on Murashige and Skoog medium. A mucilaginous, translucent embryogenic callus was obtained with 5 mg/l BA. Callus induced with 2,4-D was non-embryogenic. The embryogenic callus in liquid half strength Murashige and Skoog medium without inorganic nitrates supplemented with 2.5 g/l casein hydrolysate and 0.5 g/l L-glutamine gave rise to immature embryos. The embryos matured when treated with 60 g/l sucrose and 10 mg/l abscisic acid.

    更新日期:2019-11-01
  • Enhanced production of scopolin bySolanum aviculare cells immobilised within Ca-alginate gel beads.
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-02-01
    C Roisin,F Gillet-Manceau,J E Nava Saucedo,M Fliniaux,A Jacquin-Dubreuil,J -N Barbotin

    Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour ofS. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics ofSolanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. ImmobilisedS. aviculare have accumulated scopolin (up to 120 μg·g-1 FWB) within beads and excreted it into the culture medium (up to 8 μg·g-1 FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 μg·g-1 FWB), no scopolin has been found into the culture medium.

    更新日期:2019-11-01
  • Selection ofMycosphaerella fijiensis-resistant cell lines from micro-cross sections of banana and plantain.
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-02-01
    B N Okole,F A Schulz

    Anin vitro selection system using microcross sections of banana and plantain cultivars belonging to AAA and AAB genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin 2,4,8-trihydroxytetralone extracted from the fungusMycosphaerella fijiensis (M. fijiensis), the causal agent of Black Sigatoka disease. Resistant plantlets obtained from the double selection system were inoculated with conidia ofM. fijiensis in a growth chamber to reproduce Black Sigatoka symptoms. Compared to non-treated control plantlets, which were highly susceptible to the fungus, 10.7-19.3% toxin-resistant plantlets which arose from tissues that went through the double selection system were resistant againstM. fijiensis. This technique of using micro-cross sections for selection on fungal toxins seems to be amenable to differentMusa genotypes for the production of fungus-resistant plants.

    更新日期:2019-11-01
  • Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.).
    Plant Cell Rep. (IF 3.499) Pub Date : 1997-02-01
    Karsedis Distabanjong,Robert L Geneve

    A procedure for multiple shoot formation from somatic embryo explants of Eastern redbud (Cercis canadensis L.) cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. TDZ in combination with BA produced more shoots than either treatment alone. The highest number of shoots (3.3 to 3.4 shoots per explant) was obtained from partially desiccated and wounded explants treated with a combination of 5 or 10 μM BA and 0.5 or 1.0 μM TDZ for 20 days before being transferred to the same medium without TDZ. The number of shoots formed was increased from 1.5 to 3.2 shoots per explant by cutting through the cotyledonary node prior to culture. In addition, the frequency of explants forming shoots was increased by desiccation of somatic embryo explants to approximately 50% moisture and by using somatic embryos with two well formed cotyledons as explants.

    更新日期:2019-11-01
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