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Profiling thimet oligopeptidase‐mediated proteolysis in Arabidopsis thaliana Plant J. (IF 6.141) Pub Date : 2021-01-22 Anthony A. Iannetta; Holden T. Rogers; Thualfeqar Al‐Mohanna; Juliana N. O’Brien; Andrew J. Wommack; Sorina C. Popescu; Leslie M. Hicks
Protein homeostasis (proteostasis) is crucial for proper cellular function, including the production of peptides with biological functions through controlled proteolysis. Proteostasis has roles in maintenance of cellular functions and plant interactions with the environment under physiological conditions. Plant stress continues to reduce agricultural yields causing substantial economic losses; thus
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A cost‐effective maize ear phenotyping platform enables rapid categorization and quantification of kernels Plant J. (IF 6.141) Pub Date : 2021-01-21 Cedar Warman; Christopher M. Sullivan; Justin Preece; Michaela E. Buchanan; Zuzana Vejlupkova; Pankaj Jaiswal; John E. Fowler
High‐throughput phenotyping systems are powerful, dramatically changing our ability to document, measure, and detect biological phenomena. Here, we describe a cost‐effective combination of a custom‐built imaging platform and deep‐learning‐based computer vision pipeline. A minimal version of the Maize Ear Scanner (MES) was built with low‐cost and readily available parts. The scanner rotates a maize
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Defining natural factors that stimulate and inhibit cellulose:xyloglucan hetero‐transglucosylation Plant J. (IF 6.141) Pub Date : 2020-12-13 Klaus Herburger; Lenka Franková; Martina Pičmanová; Anzhou Xin; Frank Meulewaeter; Andrew Hudson; Stephen C. Fry
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GORI, encoding the WD40 domain protein, is required for pollen tube germination and elongation in rice Plant J. (IF 6.141) Pub Date : 2020-12-21 Yu‐Jin Kim; Myung‐Hee Kim; Woo‐Jong Hong; Sunok Moon; Eui‐Jung Kim; Jeniffer Silva; Jinwon Lee; Sangho Lee; Sun Tae Kim; Soon Ki Park; Ki‐Hong Jung
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The genome of Shanputao (Vitis amurensis) provides a new insight into cold tolerance of grapevine Plant J. (IF 6.141) Pub Date : 2020-12-10 Yi Wang; Haiping Xin; Peige Fan; Jisen Zhang; Yongbo Liu; Yang Dong; Zemin Wang; Yingzhen Yang; Qing Zhang; Ray Ming; Gan‐Yuan Zhong; Shaohua Li; Zhenchang Liang
Vitis amurensis (Shanputao) is the most cold tolerant Vitis species and so is of great interest to grape breeders and producers in areas with low winter temperatures. Here, we report its high‐quality, chromosome‐level genome assembly based on a combination of sequence data from Illumina and PacBio platforms, BioNano optical mapping and high‐throughput chromosome conformation Capture (Hi‐C) mapping
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Some characteristics of crossing over in induced recombination between chromosomes of wheat and rye Plant J. (IF 6.141) Pub Date : 2020-12-21 Chaolan Fan; Ming Hao; Zhenyu Jia; Christian Neri; Xue Chen; Wenshuai Chen; Dengcai Liu; Adam J. Lukaszewski
Allopolyploid wheat (Triticum aestivum L.) carries three pairs of homoeologous genomes but its meiotic pairing is diploid‐like. This is the effect of the Ph (pairing homoeologous) system which restricts chromosome pairing to strictly homologous. Ph1 is the locus with the strongest effect. Disabling Ph1 permits pairing between homoeologues and is routinely used in chromosome engineering to introgress
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Indole‐3‐glycerolphosphate synthase, a branchpoint for the biosynthesis of tryptophan, indole, and benzoxazinoids in maize Plant J. (IF 6.141) Pub Date : 2021-01-18 Annett Richter; Adrian F. Powell; Mahdieh Mirzaei; Lucy J. Wang; Navid Movahed; Julia K. Miller; Miguel A. Piñeros; Georg Jander
The maize (Zea mays) genome encodes three indole‐3‐glycerolphosphate synthase enzymes (IGPS1, 2, and 3) catalyzing the conversion of 1‐(2‐carboxyphenylamino)‐l‐deoxyribulose‐5‐phosphate to indole‐3‐glycerolphosphate. Three further maize enzymes (BX1, benzoxazinoneless 1; TSA, tryptophan synthase α subunit; and IGL, indole glycerolphosphate lyase) convert indole‐3‐glycerolphosphate to indole, which
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Analysis of sticky generative cell mutants reveals that suppression of callose deposition in the generative cell is necessary for generative cell internalization and differentiation in Arabidopsis Plant J. (IF 6.141) Pub Date : 2021-01-18 Sung‐Aeong Oh; Hyo‐Jin Park; Myung‐Hee Kim; Soon‐Ki Park
In flowering plants, double fertilization between male and female gametophytes, which are separated by distance, largely depends on the unique pattern of the male gametophyte (pollen): two nonmotile sperm cells suspended within a tube‐producing vegetative cell. A morphological screen to elucidate the genetic control governing the strategic patterning of pollen has led to isolation of a sticky generative
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Computational characterization of double reduction in autotetraploid natural populations Plant J. (IF 6.141) Pub Date : 2020-12-09 Libo Jiang; Xiangyu Ren; Rongling Wu
Population genetic theory has been well developed for diploid species, but its extension to study genetic diversity, variation and evolution in autopolyploids, a class of polyploids derived from the genome doubling of a single ancestral species, requires the incorporation of multisomic inheritance. Double reduction, which is characteristic of autopolyploidy, has long been believed to shape the evolutionary
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Aethionema arabicum genome annotation using PacBio full‐length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research Plant J. (IF 6.141) Pub Date : 2021-01-16 Noe Fernandez‐Pozo; Timo Metz; Jake O. Chandler; Lydia Gramzow; Zsuzsanna Mérai; Florian Maumus; Ortrun Mittelsten Scheid; Günter Theißen; M. Eric Schranz; Gerhard Leubner‐Metzger; Stefan A. Rensing
Aethionema arabicum is an important model plant for Brassicaceae trait evolution, particularly of seed (development, regulation, germination, dormancy) and fruit (development, dehiscence mechanisms) characters. Its genome assembly was recently improved but the gene annotation was not updated. Here, we improved the Ae. arabicum gene annotation using 294 RNA‐seq libraries and 136,307 full‐length PacBio
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Tomato protein Rx4 mediates the hypersensitive response to Xanthomonas euvesicatoria pv. perforans race T3 Plant J. (IF 6.141) Pub Date : 2020-12-20 Xiaofei Zhang; Ning Li; Xin Liu; Jiajing Wang; Yaxian Zhang; Dong Liu; Yuqing Wang; Haipeng Cao; Baimei Zhao; Wencai Yang
Bacterial spot, which is caused by several Xanthomonas species, is an economically important disease in tomato (Solanum lycopersicum). Great efforts have been made for the identification of resistant sources and the genetic analysis of resistance. However, the development of resistant commercial varieties is slow due to the existence of multiple species of the pathogen and a poor understanding of the
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MLK4‐mediated phosphorylation of histone H3T3 promotes flowering by transcriptional silencing of FLC/MAF in Arabidopsis thaliana Plant J. (IF 6.141) Pub Date : 2020-12-06 Zhen Wang; Junmei Kang; Juan Armando Casas‐Mollano; Yongchao Dou; Shangang Jia; Qingchuan Yang; Chi Zhang; Heriberto Cerutti
Casein kinase I (CK1), a ubiquitous Ser/Thr protein kinase in eukaryotes, plays a critical role in higher plant flowering. Arabidopsis CK1 family member MUT9‐LIKE KINASEs, such as MLK1 and MLK3, have been shown to phosphorylate histone H3 at threonine 3 (H3T3), an evolutionarily conserved residue, and the modification is associated with the transcriptional repression of euchromatic and heterochromatic
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Flowering time gene or jack of all trades? Plant J. (IF 6.141) Pub Date : 2021-01-16 Leonie Verhage
What is the definition of a flowering time gene? The answer is more complicated than you might think. Of course, flowering time genes control the transition from the vegetative to the reproductive phase. But there is increasing evidence that many of these genes have much broader functions (Auge et al., 2019). For example, FLOWERING LOCUS C (FLC), known for its role in repressing the floral transition
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Emb15 encodes a plastid ribosomal assembly factor essential for embryogenesis in maize Plant J. (IF 6.141) Pub Date : 2021-01-15 Chunhui Xu; Yun Shen; Cuiling Li; Fan Lu; Meng‐Di Zhang; Robert B. Meeley; Donald R. McCarty; Bao‐Cai Tan
Ribosome assembly factors guide the complex process by which ribosomal proteins and the ribosomal RNAs form a functional ribosome. However, the assembly of plant plastid ribosomes is poorly understood. In the present study, we discovered a maize (Zea mays) plastid ribosome assembly factor based on our characterization of the embryo defective 15 (emb15) mutant. Loss of function of Emb15 retards embryo
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Phytohormones in fruit development and maturation Plant J. (IF 6.141) Pub Date : 2020-12-04 Matthew A. Fenn; James J. Giovannoni
Phytohormones are integral to the regulation of fruit development and maturation. This review expands upon current understanding of the relationship between hormone signaling and fruit development, emphasizing fleshy fruit and highlighting recent work in the model crop tomato (Solanum lycopersicum) and additional species. Fruit development comprises fruit set initiation, growth, and maturation and
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Genome‐wide occupancy of Arabidopsis SWI/SNF chromatin remodeler SPLAYED provides insights into its interplay with its close homolog BRAHMA and Polycomb proteins Plant J. (IF 6.141) Pub Date : 2021-01-11 Jie Shu; Chen Chen; Chenlong Li; Raj K Thapa; Jingpu Song; Xin Xie; Vi Nguyen; Shaomin Bian; Jun Liu; Susanne E Kohalmi; Yuhai Cui
SPLAYED (SYD) is a SWItch/Sucrose Non‐Fermentable (SWI/SNF)‐type chromatin remodeler identified in Arabidopsis thaliana (Arabidopsis). It is believed to play both redundant and differential roles with its closest homolog BRAHMA (BRM) in diverse plant growth and development processes. To better understand how SYD functions, we profiled the genome‐wide occupancy of SYD and its impact on the global transcriptome
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Whole‐genome assembly and resequencing reveal genomic imprint and key genes of rapid domestication in narrow‐leafed lupin Plant J. (IF 6.141) Pub Date : 2020-11-29 Penghao Wang; Gaofeng Zhou; Jianbo Jian; Huaan Yang; Daniel Renshaw; Matthew K. Aubert; Jonathan Clements; Tianhua He; Mark Sweetingham; Chengdao Li
Shifting from a livestock‐based protein diet to a plant‐based protein diet has been proposed as an essential requirement to maintain global food sustainability, which requires the increased production of protein‐rich crops for direct human consumption. Meanwhile, the lack of sufficient genetic diversity in crop varieties is an increasing concern for sustainable food supplies. Countering this concern
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MdABI5 works with its interaction partners to regulate abscisic acid‐mediated leaf senescence in apple Plant J. (IF 6.141) Pub Date : 2020-12-13 Jian‐Ping An; Xiao‐Wei Zhang; Ya‐Jing Liu; Jiu‐Cheng Zhang; Xiao‐Fei Wang; Chun‐Xiang You; Yu‐Jin Hao
Abscisic acid (ABA) induces chlorophyll degradation and leaf senescence; however, the molecular mechanism remains poorly understood, especially in woody plants. In this study, we found that MdABI5 plays an essential role in the regulation of ABA‐triggered leaf senescence in Malus domestica (apple). Through yeast screening, three transcription factors, MdBBX22, MdWRKY40 and MdbZIP44, were found to interact
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Wheat 2‐Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature Plant J. (IF 6.141) Pub Date : 2020-12-07 Divya Mishra; Shubhendu Shekhar; Subhra Chakraborty; Niranjan Chakraborty
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Gene atlas of iron‐containing proteins in Arabidopsis thaliana Plant J. (IF 6.141) Pub Date : 2021-01-10 Jonathan Przybyla‐Toscano; Clément Boussardon; Simon Law; Nicolas Rouhier; Olivier Keech
Iron (Fe) is an essential element for the development and physiology of plants owing to its presence in numerous proteins involved in central biological processes. Here, we established an exhaustive, manually curated inventory of genes encoding Fe‐containing proteins in Arabidopsis thaliana, and summarised their subcellular localisation, spatiotemporal expression, and evolutionary age. We have currently
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Flourishing in Water: The Early Evolution and Diversification of Plant Receptor‐like Kinases Plant J. (IF 6.141) Pub Date : 2021-01-10 Zhen Gong; Guan‐Zhu Han
Receptor‐like kinases (RLKs) play significant roles in mediating innate immunity and development of plants. The evolution of plant RLKs has been characterized by extensive variation in copy numbers and domain configurations. However, much remains unknown about the origin, evolution, and early diversification of plant RLKs. Here, we perform phylogenomic analyses of RLKs across plants (Archaeplastida)
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Unveiling the spatial distribution of aflatoxin B1 and plant defense metabolites in maize using AP‐SMALDI mass spectrometry imaging Plant J. (IF 6.141) Pub Date : 2021-01-09 Laura Righetti; Dhaka Ram Bhandari; Enrico Rolli; Sara Tortorella; Renato Bruni; Chiara Dall’Asta; Bernhard Spengler
To cope with the presence of unfavorable compounds, plants are capable to biotransform xenobiotics, translocate both parent compounds and metabolites, perform compartmentation and segregation at cellular or tissue level. Such a scenario also applies to mycotoxins, fungal secondary metabolites with a preeminent role in plant infection. In this work, we aimed to describe the effect of the interplay between
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The function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis Plant J. (IF 6.141) Pub Date : 2021-01-09 John Vaughan‐Hirsch; Emily J. Tallerday; Christian A. Burr; Charlie Hodgens; Samantha Louise Boeshore; Kevin Beaver; Allison Melling; Kartika Sari; Ian D. Kerr; Jan Šimura; Karin Ljung; Dawei Xu; Wanqi Liang; Rahul Bhosale; G. Eric Schaller; Anthony Bishopp; Joseph J. Kieber
The phytohormone cytokinin plays a significant role in nearly all aspects of plant growth and development. Cytokinin signaling has primarily been studied in the dicot model Arabidopsis, with relatively little work done in monocots, which include rice (Oryza sativa) and other cereals of agronomic importance. The cytokinin signaling pathway is a phosphorelay comprised of the histidine kinase receptors
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Nucleolar rDNA folds into condensed foci with a specific combination of epigenetic marks Plant J. (IF 6.141) Pub Date : 2020-12-12 Konstantin O. Kutashev; Michal Franek; Klev Diamanti; Jan Komorowski; Marie Olšinová; Martina Dvořáčková
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RAB GTPASE HOMOLOG 8D is required for the maintenance of both the root stem cell niche and the meristem Plant J. (IF 6.141) Pub Date : 2020-12-01 Pengcheng Li; Junjie Ma; Xueping Sun; Chuanzhi Zhao; Changle Ma; Xingjun Wang
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Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant Plant J. (IF 6.141) Pub Date : 2020-12-01 Xinyu Liu; Rebecca Van Acker; Wannes Voorend; Andreas Pallidis; Geert Goeminne; Jacob Pollier; Kris Morreel; Hoon Kim; Hilde Muylle; Mickael Bosio; John Ralph; Ruben Vanholme; Wout Boerjan
Lignocellulosic biomass is an abundant byproduct from cereal crops that can potentially be valorized as a feedstock to produce biomaterials. Zea mays CINNAMYL ALCOHOL DEHYDROGENASE 2 (ZmCAD2) is involved in lignification, and is a promising target to improve the cellulose‐to‐glucose conversion of maize stover. Here, we analyzed a field‐grown zmcad2 Mutator transposon insertional mutant. Zmcad2 mutant
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Endogenous gibberellins affect root nodule symbiosis via transcriptional regulation of NODULE INCEPTION in Lotus japonicus Plant J. (IF 6.141) Pub Date : 2020-12-10 Akira Akamatsu; Miwa Nagae; Yuka Nishimura; Daniela Romero Montero; Satsuki Ninomiya; Mikiko Kojima; Yumiko Takebayashi; Hitoshi Sakakibara; Masayoshi Kawaguchi; Naoya Takeda
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Designing future crops: challenges and strategies for sustainable agriculture Plant J. (IF 6.141) Pub Date : 2020-12-01 Zhixi Tian; Jia‐Wei Wang; Jiayang Li; Bin Han
Crop production is facing unprecedented challenges. Despite the fact that the food supply has significantly increased over the past half‐century, ~8.9 and 14.3% people are still suffering from hunger and malnutrition, respectively. Agricultural environments are continuously threatened by a booming world population, a shortage of arable land, and rapid changes in climate. To ensure food and ecosystem
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Arabidopsis Abscisic Acid Repressor 1 is a susceptibility hub that interacts with multiple Pseudomonas syringae effectors Plant J. (IF 6.141) Pub Date : 2020-12-02 Karl J. Schreiber; Jana A. Hassan; Jennifer D. Lewis
Pathogens secrete effector proteins into host cells to suppress host immunity and promote pathogen virulence, although many features at the molecular interface of host–pathogen interactions remain to be characterized. In a yeast two‐hybrid assay, we found that the Pseudomonas syringae effector HopZ1a interacts with the Arabidopsis transcriptional regulator Abscisic Acid Repressor 1 (ABR1). Further
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Plant apocarotenoids: from retrograde signaling to interspecific communication Plant J. (IF 6.141) Pub Date : 2020-11-30 Juan C. Moreno; Jianing Mi; Yagiz Alagoz; Salim Al‐Babili
Carotenoids are isoprenoid compounds synthesized by all photosynthetic and some non‐photosynthetic organisms. They are essential for photosynthesis and contribute to many other aspects of a plant's life. The oxidative breakdown of carotenoids gives rise to the formation of a diverse family of essential metabolites called apocarotenoids. This metabolic process either takes place spontaneously through
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Ligand–receptor interactions in plant hormone signaling Plant J. (IF 6.141) Pub Date : 2020-12-05 Jun Takeuchi; Kosuke Fukui; Yoshiya Seto; Yousuke Takaoka; Masanori Okamoto
Small‐molecule plant hormones principally control plant growth, development, differentiation, and environmental responses. Nine types of plant hormones are ubiquitous in angiosperms, and the molecular mechanisms of their hormone actions have been elucidated during the last two decades by genomic decoding of model plants with genetic mutants. In particular, the discovery of hormone receptors has greatly
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A chromosome‐scale genome assembly of European hazel (Corylus avellana L.) reveals targets for crop improvement Plant J. (IF 6.141) Pub Date : 2020-11-29 Stuart J. Lucas; Kadriye Kahraman; Bihter Avşar; Richard J.A. Buggs; Ipek Bilge
The European hazelnut (Corylus avellana L.) is a tree crop of economic importance worldwide, but especially for northern Turkey, where the majority of production takes place. Hazelnut production is currently challenged by environmental stresses, such as a recent outbreak of severe powdery mildew disease; furthermore, allergy to hazelnuts is an increasing health concern in some regions. In order to
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Two atypical AUGUSTIFOLIA without a plant‐specific C‐terminus regulate gametophore and sporophyte shapes in the moss Physcomitrium (Physcomitrella) patens Plant J. (IF 6.141) Pub Date : 2020-12-06 Katsuaki Takechi; Hiroaki Nagase; Tomoyuki Furuya; Koro Hattori; Yoshikatsu Sato; Kensuke Miyajima; Tomofumi Higuchi; Ryuya Matsuda; Susumu Takio; Hirokazu Tsukaya; Hiroyoshi Takano
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Visualizing vinca alkaloids in the petal of Catharanthus roseus using functionalized titanium oxide nanowire substrate for surface‐assisted laser desorption/ionization imaging mass spectrometry Plant J. (IF 6.141) Pub Date : 2020-11-21 Ewelina P. Dutkiewicz; Chun‐Han Su; Han‐Jung Lee; Cheng‐Chih Hsu; Yu‐Liang Yang
Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at a high spatial resolution with low detection limits. In contrast to the matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) approach, surface‐assisted laser desorption/ionization (SALDI) can be more effective in the detection of small molecules due to the absence of interfering
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Dimerization of the ETO1 family proteins plays a crucial role in regulating ethylene biosynthesis in Arabidopsis thaliana Plant J. (IF 6.141) Pub Date : 2020-12-03 Shin‐Yuan Gu; Wan‐Sheng Lo; Shaw‐Jye Wu; Long‐Chi Wang
ETHYLENE OVERPRODUCER1 (ETO1), ETO1‐LIKE1 (EOL1), and EOL2 are members of the Broad complex, Tramtrack, Bric‐a‐brac (BTB) protein family that collectively regulate type‐2 1‐aminocyclopropane‐1‐carboxylic acid synthase (ACS) activity in Arabidopsis thaliana. Although ETO1 and EOL1/EOL2 encode structurally related proteins, genetic studies suggest that they do not play an equivalent role in regulating
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Chromoplast differentiation in bell pepper (Capsicum annuum) fruits Plant J. (IF 6.141) Pub Date : 2020-11-30 Anja Rödiger; Birgit Agne; Dirk Dobritzsch; Stefan Helm; Fränze Müller; Nina Pötzsch; Sacha Baginsky
We report here a detailed analysis of the proteome adjustments that accompany chromoplast differentiation from chloroplasts during bell pepper (Capsicum annuum) fruit ripening. While the two photosystems are disassembled and their constituents degraded, the cytochrome b6f complex, the ATPase complex, and Calvin cycle enzymes are maintained at high levels up to fully mature chromoplasts. This is also
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Lipids in xylem sap of woody plants across the angiosperm phylogeny Plant J. (IF 6.141) Pub Date : 2020-12-09 H. Jochen Schenk; Joseph M. Michaud; Kerri Mocko; Susana Espino; Tatiana Melendres; Mary R. Roth; Ruth Welti; Lucian Kaack; Steven Jansen
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Histone H3K4 methyltransferases SDG25 and ATX1 maintain heat‐stress gene expression during recovery in Arabidopsis Plant J. (IF 6.141) Pub Date : 2020-12-05 Ze‐Ting Song; Lin‐Lin Zhang; Jia‐Jia Han; Ming Zhou; Jian‐Xiang Liu
Plants have short‐term stress memory that enables them to maintain the expression state of a substantial subset of heat‐inducible genes during stress recovery after heat stress. Little is known about the molecular mechanisms controlling stress‐responsive gene expression at the recovery stage in plants, however. In this article, we demonstrate that histone H3K4 methyltransferases SDG25 and ATX1 are
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The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus Plant J. (IF 6.141) Pub Date : 2020-12-02 Yuanzhong Jiang; Shaofei Tong; Ningning Chen; Bao Liu; Qiuxian Bai; Yang Chen; Hao Bi; Zhiyang Zhang; Shangling Lou; Hu Tang; Jianquan Liu; Tao Ma; Huanhuan Liu
High salinity, one of the most widespread abiotic stresses, inhibits photosynthesis, reduces vegetation growth, blocks respiration and disrupts metabolism in plants. In order to survive their long‐term lifecycle, trees, such as Populus species, recruit the abscisic acid (ABA) signaling pathway to adapt to a saline environment. However, the molecular mechanism behind the ABA‐mediated salt stress response
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Parallelism in gene expression between foothill and alpine ecotypes in Arabidopsis arenosa Plant J. (IF 6.141) Pub Date : 2020-12-01 Guillaume Wos; Magdalena Bohutínská; Jana Nosková; Terezie Mandáková; Filip Kolář
Parallel adaptation results from the independent evolution of similar traits between closely related lineages and allows us to test to what extent evolution is repeatable. Similar gene expression changes are often detected but the identity of genes shaped by parallel selection and the causes of expression parallelism remain largely unknown. By comparing genomes and transcriptomes of four distinct foothill–alpine
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Distance‐based measurement determines the coexistence of B protein hetero‐ and homodimers in lily tepal and stamen tetrameric complexes Plant J. (IF 6.141) Pub Date : 2020-12-05 Wan‐Ting Mao; Wei‐Han Hsu; Jen‐Ying Li; Chang‐Hsien Yang
The floral quartet model proposes that plant MADS box proteins function as higher order tetrameric complexes. However, in planta evidence for MADS box tetramers remains scarce. Here, we applied a strategy using in vivo fluorescence resonance energy transfer (FRET) based on the distance change and distance symmetry of stable tetrameric complexes in tobacco (Nicotiana benthamiana) leaf cells to improve
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A single cytochrome P450 oxidase from Solanum habrochaites sequentially oxidizes 7‐epi‐zingiberene to derivatives toxic to whiteflies and various microorganisms Plant J. (IF 6.141) Pub Date : 2020-12-04 Sebastian Zabel; Wolfgang Brandt; Andrea Porzel; Benedikt Athmer; Stefan Bennewitz; Petra Schäfer; Ruy Kortbeek; Petra Bleeker; Alain Tissier
Secretions from glandular trichomes potentially protect plants against a variety of aggressors. In the tomato clade of the Solanum genus, glandular trichomes of wild species produce a rich source of chemical diversity at the leaf surface. Previously, 7‐epi‐zingiberene produced in several accessions of Solanum habrochaites was found to confer resistance to whiteflies (Bemisia tabaci) and other insect
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Protein phosphorylation regulates maize endosperm starch synthase IIa activity and protein−protein interactions Plant J. (IF 6.141) Pub Date : 2020-11-24 Sahar Mehrpouyan; Usha Menon; Ian J. Tetlow; Michael J. Emes
Starch synthesis is an elaborate process employing several isoforms of starch synthases (SSs), starch branching enzymes (SBEs) and debranching enzymes (DBEs). In cereals, some starch biosynthetic enzymes can form heteromeric complexes whose assembly is controlled by protein phosphorylation. Previous studies suggested that SSIIa forms a trimeric complex with SBEIIb, SSI, in which SBEIIb is phosphorylated
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Reconstitution of oxaloacetate metabolism in the tricarboxylic acid cycle in Synechocystis sp. PCC 6803: discovery of important factors that directly affect the conversion of oxaloacetate Plant J. (IF 6.141) Pub Date : 2020-12-06 Shoki Ito; Takumi Hakamada; Tatsumi Ogino; Takashi Osanai
The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathways in nature. Oxygenic photoautotrophic bacteria, cyanobacteria, have an unusual TCA cycle. The TCA cycle in cyanobacteria contains two unique enzymes that are not part of the TCA cycle in other organisms. In recent years, sustainable metabolite production from carbon dioxide using cyanobacteria has been looked at as a
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Phospholipase D‐ and phosphatidic acid‐mediated phospholipid metabolism and signaling modulate symbiotic interaction and nodulation in soybean (Glycine max) Plant J. (IF 6.141) Pub Date : 2020-12-29 Gaoyang Zhang; Jihong Yang; Xiangli Chen; Dandan Zhao; Xiuhong Zhou; Yuliang Zhang; Xuemin Wang; Jian Zhao
Symbiotic rhizobium‐legume interactions, such as root hair curling, rhizobial invasion, infection thread expansion, cell division and proliferation of nitrogen‐fixing bacteroids, and nodule formation, involve extensive membrane synthesis, lipid remodeling, and cytoskeleton dynamics. However, little is known about these membrane‐cytoskeleton interfaces and related genes. Here, we report the roles of
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Centromere organization and UU/V sex chromosome behavior in a liverwort Plant J. (IF 6.141) Pub Date : 2020-12-29 Aretuza Sousa; Veit Schubert; Susanne S. Renner
In 1917, sex chromosomes in plants were discovered in a liverwort with hetermorphic U and V chromosomes. Such hetermorphy is unexpected because, different from XY chromosomes in diploid‐dominant plants, in haploid‐dominants, the female U and the male V experience largely symmetrical potential recombination environments. We here use molecular cytogenetics and super‐resolution microscopy to study Frullania
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Functional studies of CpSRP54 in diatoms show that the mechanism of thylakoid protein insertion differs from plants and green algae Plant J. (IF 6.141) Pub Date : 2020-12-29 Marianne Nymark; Marthe Caroline Grønbech Hafskjold; Charlotte Volpe; Davi de Miranda Fonseca; Animesh Sharma; Eirini Tsirvouli; Manuel Serif; Per Winge; Giovanni Finazzi; Atle Magnar Bones
The chloroplast signal recognition particle 54 kDa (CpSRP54) protein is a member of the CpSRP pathway known to target proteins to thylakoid membranes in plants and green algae. Loss of CpSRP54 in the marine diatom Phaeodactylum tricornutum lowers the accumulation of a selection of chloroplast encoded subunits of photosynthetic complexes, indicating a role in the co‐translational part of the CpSRP pathway
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Exploring the alpha‐gliadin locus: the 33‐mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii Plant J. (IF 6.141) Pub Date : 2020-12-28 Jan G Schaart; Elma MJ Salentijn; Svetlana V Goryunova; Charity Chidzanga; Danny G Esselink; Nick Gosman; Alison R Bentley; Luud JWJ Gilissen; Marinus JM Smulders
Most alpha‐gliadin genes of the Gli‐D2 locus on the D genome of hexaploid bread wheat (Triticum aestivum) encode for proteins with epitopes that can trigger coeliac disease (CD), and several contain a 33‐mer peptide with six partly overlapping copies of three epitopes, which is regarded as a remarkably potent T cell stimulator. To increase genetic diversity in the D genome, synthetic hexaploid wheat
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Mutations in Tomato 1‐Aminocyclopropane Carboxylic Acid Synthase2 Uncover Its Role in Development beside Fruit Ripening Plant J. (IF 6.141) Pub Date : 2020-12-28 Kapil Sharma; Soni Gupta; Supriya Sarma; Meenakshi Rai; Yellamaraju Sreelakshmi; Rameshwar Sharma
The role of ethylene in plant development is mostly inferred from its exogenous application. The usage of the mutants affecting ethylene biosynthesis proffers a better alternative to decipher its role. In tomato, 1‐aminocyclopropane carboxylic acid synthase2 (ACS2) is a key enzyme regulating ripening‐specific ethylene biosynthesis. We characterized two contrasting acs2 mutants; acs2‐1 overproduces
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Foliar water and solute absorption: an update Plant J. (IF 6.141) Pub Date : 2020-11-21 Victoria Fernández; Eustaquio Gil‐Pelegrín; Thomas Eichert
The absorption of water and solutes by plant leaves has been recognised since more than two centuries. Given the polar nature of water and solutes, the mechanisms of foliar uptake have been proposed to be similar for water and electrolytes, including nutrient solutions. Research efforts since the 19th century focussed on characterising the properties of cuticles and applying foliar sprays to crop plants
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DNA methylation dynamics of sperm cell lineage development in tomato Plant J. (IF 6.141) Pub Date : 2020-11-29 Yunlong Lu; Yunyun Song; Lingtong Liu; Tai Wang
During the sexual reproduction of higher plants, DNA methylation and transcription are broadly changed to reshape a microspore into two sperm cells (SCs) and a vegetative cell (VC). However, when and how the DNA methylation of SCs is established remains not fully understood. Here we investigate the DNA methylation (5 mC) dynamics of SC lineage and the VC in tomato using whole‐genome bisulfite sequencing
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Functional requirement of the Arabidopsis importin‐α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1 Plant J. (IF 6.141) Pub Date : 2020-11-19 Daniel Lüdke; Charlotte Roth; Sieglinde A. Kamrad; Jana Messerschmidt; Denise Hartken; Jonas Appel; Bojan F. Hörnich; Qiqi Yan; Stefan Kusch; Melanie Klenke; Annette Gunkel; Lennart Wirthmueller; Marcel Wiermer
IMPORTIN‐α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin‐α isoforms in Arabidopsis that recruit nuclear localization signal‐containing cargo proteins to the nuclear import machinery. IMP‐α3/MOS6 is required genetically for full autoimmunity of the nucleotide‐binding leucine‐rich repeat immune receptor mutant snc1 (suppressor of npr1‐1, constitutive 1) and MOS6 also contributes to basal disease
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OsmiR164‐targeted OsNAM, a boundary gene, plays important roles in rice leaf and panicle development Plant J. (IF 6.141) Pub Date : 2020-12-23 Zhongyuan Chang; Ruihan Xu; Qian Xun; Jiajun Liu; Tianhui Zhong; Yanfeng Ding; Chengqiang Ding
The CUP‐SHAPED COTYLEDON (CUC) genes (CUC1, CUC2, and CUC3) regulate organ boundary formation in Arabidopsis. However, the functions of their homologous genes in rice (Oryza sativa) are still unknown. Here, we have identified an orthologous gene of CUC1 and CUC2 in rice named OsNAM. Subcellular localization and yeast two‐hybrid assay results have suggested that OsNAM encodes a conserved nuclear NAC
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Acclimation in plants – the Green Hub consortium Plant J. (IF 6.141) Pub Date : 2020-12-23 Tatjana Kleine; Thomas Nägele; H. Ekkehard Neuhaus; Christian Schmitz‐Linneweber; Alisdair R. Fernie; Peter Geigenberger; Bernhard Grimm; Kerstin Kaufmann; Edda Klipp; Jörg Meurer; Torsten Möhlmann; Timo Mühlhaus; Belen Naranjo; Jörg Nickelsen; Andreas Richter; Hannes Ruwe; Michael Schroda; Serena Schwenkert; Oliver Trentmann; Felix Willmund; Reimo Zoschke; Dario Leister
Acclimation is the capacity to adapt to environmental changes within the life time of an individual. This ability allows plants to cope with the continuous variation in ambient conditions to which they are exposed as sessile organisms. Because environmental changes and extremes are becoming even more pronounced due to the current period of climate change, enhancing the efficacy of plant acclimation
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On the outside looking in: roles of endogenous and exogenous strigolactones Plant J. (IF 6.141) Pub Date : 2020-11-20 Bruno Aquino; James M. Bradley; Shelley Lumba
A collection of small molecules called strigolactones (SLs) act as both endogenous hormones to control plant development and as ecological communication cues between organisms. SL signalling overlaps with that of a class of smoke‐derived compounds, karrikins (KARs), which have distinct yet overlapping developmental effects on plants. Although the roles of SLs in shoot and root development, in the promotion
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Phytochrome B inhibits darkness‐induced hypocotyl adventitious root formation by stabilizing IAA14 and suppressing ARF7 and ARF19 Plant J. (IF 6.141) Pub Date : 2020-12-23 Qian‐Qian Li; Zhan Zhang; Ya‐Ling Wang; Li‐Yuan Zhong; Zhen‐Fei Chao; Yi‐Qun Gao; Mei‐Ling Han; Lin Xu; Dai‐Yin Chao
Adventitious roots (ARs) are an important root type for plants and display a high phenotypic plasticity in response to different environmental stimuli. Previous studies found that dark‐light transition can trigger AR formation from the hypocotyl of etiolated Arabidopsis thaliana, which was used as model for identification of regulators of AR biogenesis. However, the central regulatory machinery for
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Proposed mechanism for regulation of H2O2‐induced programmed cell death in plants by binding of cytochrome c to 14‐3‐3 proteins Plant J. (IF 6.141) Pub Date : 2020-12-23 Carlos A. Elena‐Real; Katiuska González‐Arzola; Gonzalo Pérez‐Mejías; Antonio Díaz‐Quintana; Adrián Velázquez‐Campoy; Bénédicte Desvoyes; Crisanto Gutiérrez; Miguel A. De la Rosa; Irene Díaz‐Moreno
Programmed cell death is crucial for development and homeostasis of all living organisms. In human cells, the double role of extra‐mitochondrial cytochrome c in triggering apoptosis and inhibiting survival pathways is well reported. In plants, however, the specific role of cytochrome c upon release from the mitochondria remains in part veiled yet death stimuli do trigger cytochrome c translocation
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Disruption of NAP1 genes in Arabidopsis thaliana suppresses the fas1 mutant phenotype, enhances genome stability and changes the chromatin compaction Plant J. (IF 6.141) Pub Date : 2020-12-23 Karolína Kolářová; Martina Nešpor Dadejová; Tomáš Loja; Gabriela Lochmanová; Eva Sýkorová; Martina Dvořáčková
Histone chaperones mediate assembly and disassembly of nucleosomes and participate in essentially all DNA‐dependent cellular processes. In Arabidopsis thaliana, loss‐of‐functions of FAS1 or FAS2 subunits of the H3‐H4 histone chaperone complex CHROMATIN ASSEMBLY FACTOR 1 (CAF‐1) has a dramatic effect on plant morphology, growth and overall fitness. Altered chromatin compaction, systematic loss of repetitive
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Validated MAGIC and GWAS population mapping reveals the link between vitamin E content and natural variation in chorismate metabolism in tomato Plant J. (IF 6.141) Pub Date : 2020-11-12 Estanislao Burgos; Maria Belen De Luca; Isidore Diouf; Luis A. de Haro; Elise Albert; Christopher Sauvage; Zhao J. Tao; Luisa Bermudez; Ramon Asís; Adriano N. Nesi; Michel Matringe; Claire Bréhélin; Thomas Guiraud; Carine Ferrand; Isabelle Atienza; Joana Jorly; Jean P. Mauxion; Pierre Baldet; Alisdair R. Fernie; Leandro Quadrana; Christophe Rothan; Mathilde Causse; Fernando Carrari
Tocochromanols constitute the different forms of vitamin E (VTE), essential components of the human diet, and display a high membrane protectant activity. By combining interval mapping and genome‐wide association studies (GWAS), we unveiled the genetic determinants of tocochromanol accumulation in tomato (Solanum lycopersicum) fruits. To enhance the nutritional value of this highly consumed vegetable