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Dorsoventrally asymmetric expression of miR319/TCP generates dorsal-specific venation patterning in petunia corolla tube J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-16 Bin Zhang, Xiaoting Qin, Yao Han, Mingyang Li, Yulong Guo
Vein-associated pigmentation (Venation) is a type of floral coloration adopted by plants to attract pollinators. Several petunia (Petunia hybrida) lines generate dorsoventrally asymmetric venation patterning of the corolla tube, in which venation is only presents in the dorsal tube. The molecular mechanism underlying this trait is unknown. Here, we demonstrate that miR319 is preferentially expressed
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A tomato B-box protein regulates plant development and fruit quality through the interaction with PIF4, HY5 and RIN transcription factors J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-16 Lumi Shiose, Juliene dos Reis Moreira, Bruno Silvestre Lira, Gabriel Ponciano, Gabriel Gomez Ocampo, Raquel Tsu Ay Wu, José Laurindo dos Santos Júnior, Nikolaos Ntelkis, Elke Clicque, Maria José Oliveira, Greice Lubini, Eny Iochevet Segal Floh, Javier Francisco Botto, Marcelo José Pena Ferreira, Alain Goossens, Luciano Freschi, Magdalena Rossi
During the last decade, the knowledge about BBX proteins has abruptly increased. Genome-wide studies identified BBX gene family in several ornamental, industry and food crops; however, the reports regarding the role of these genes as regulators of agronomically important traits are scarce. Here, by phenotyping a knockout mutant, we performed a comprehensive functional characterization of the tomato
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Monosaccharide transporter OsMST6 is activated by transcription factor OsERF120 to enhance chilling tolerance in rice seedlings J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-15 Shengtao Luo, Shuangshuang Zheng, Zhitao Li, Jie Cao, Bo Wang, Yunyuan Xu, Kang Chong
Chilling stress caused by extreme weather is threatening global rice (Oryza sativa L.) production. Identifying components of the signal transduction pathways underlying chilling tolerance in rice would advance molecular breeding. Here, we report that OsMST6, which encodes a monosaccharide transporter, positively regulates the chilling tolerance of rice seedlings. The mst6 mutants showed hypersensitivity
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SlGolS2 influences the metabolism of chlorophyll, carotenoid, and ethylene in tomato fruits J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-15 Huidong Zhang, Kunpeng Zhang, Xueya Zhao, Mengxi Bi, Yudong Liu, Shuo Wang, Yi He, Kui Ma, Mingfang Qi
Galactinol synthase (GolS), which catalyzes the synthesis of galactinol, is the first critical enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs) and contributes to plant growth and development, and resistance mechanisms. However, its role in fruit development remains largely unknown. In this study, we used CRISPR-Cas9 gene editing technology to create the slgols2 mutation showing
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S-locus F-Box Protein as Pollen S determinant Targets Non-self S-RNase underlying Self-Incompatibility in Citrus J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-15 Zong-Hong Cao, Dan Song, Yu Hu, Mei Liang, Qiang Xu, Shao-Hua Wang, Jun-Li Ye, Zong-Zhou Xie, Xiu-Xin Deng, Li-Jun Chai
Self-incompatibility (SI) is a crucial mechanism that prevents self-fertilization and inbreeding in flowering plants. Citrus exhibits SI regulated by a polymorphic S-locus containing an S-RNase gene and multiple S-locus F-box (SLF) genes. It has been documented that S-RNase functions as the pistil S determinant, but there is no direct evidence that the SLFs closely linked with S-RNase function as pollen
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“Alternative Pathways Leading to Ascorbate Biosynthesis in Plants: Lessons from the Last Twenty-five Years” J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-14 Cherryl O Quiñones, Reinier Gesto-Borroto, Rachael V Wilson, Sara V Hernández-Madrigal, Argelia Lorence
L-Ascorbic acid (AsA) is an antioxidant with important roles in plant stress physiology, growth and development. AsA also plays an essential role in human health, preventing scurvy. Humans do not synthesize AsA, which needs to be supplied via a diet rich in fresh produce. Research efforts have provided progress in the elucidation of a complex metabolic network with at least four routes leading to AsA
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Changes in carbohydrate distribution of cotton and increase in boll weight reduced yield loss under high temperature J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-14 Liuyan Yang, Jiahao Duan, Yu Liu, Wei Hu, Xingke Liu, Youhua Wang, Zhiguo Zhou, Wenqing Zhao
Cotton yield is sometimes unresponsive to high temperature (HT) that induce significant reductions in fruit retention. To investigate the underlying mechanisms, a greenhouse experiment was conducted with two temperature regimes (control, control treatment, 28 °C; HT, 34 °C) for 7 days. Results showed HT did not significantly influence cotton yield, but remarkably reduce boll number and increase boll
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Phylogenetically diverse wild plant species use common biochemical strategies to thrive in the Atacama Desert J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-12 Thomas Dussarrat, Ricardo Nilo-Poyanco, Tomás C Moyano, Sylvain Prigent, Tim L Jeffers, Francisca P Díaz, Guillaume Decros, Lauren Audi, Veronica M Sondervan, Bingran Shen, Viviana Araus, Dominique Rolin, Dennis Shasha, Gloria M Coruzzi, Yves Gibon, Claudio Latorre, Pierre Pétriacq, Rodrigo A Gutiérrez
The best ideotypes are under mounting pressure due to increased aridity. Understanding the conserved molecular mechanisms that evolve in wild plants adapted to harsh environments is crucial in developing new strategies for agriculture. Yet our knowledge of such mechanisms in wild species is scant. We performed metabolic pathway reconstruction using transcriptome information from 32 Atacama and phylogenetically
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Words of caution regarding T-DNA-associated mutagenesis in plant reproduction research J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-12 Karel Raabe, Limin Sun, Cédric Schindfessel, David Honys, Danny Geelen
T-DNA transformation is prevalent in Arabidopsis research and has expanded to a broad range of crops and model plants. While major progress has been made in optimizing the Agrobacterium-transformation process for various species, a variety of pitfalls associated with the T-DNA insertion may lead to the misinterpretation of T-DNA mutant analysis. Indeed, secondary mutagenesis either on the integration
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Anthocyanins act as a sugar-buffer and an alternative electron sink in response to starch depletion during leaf senescence: a case study on a typical anthocyanic tree species, Acer japonicum J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-12 Mitsutoshi Kitao, Kenichi Yazaki, Hiroyuki Tobita, Evgenios Agathokleous, Junko Kishimoto, Atsushi Takabayashi, Ryouichi Tanaka
We hypothesized that anthocyanins act as a sugar-buffer and an alternative electron sink during leaf senescence to prevent sugar-mediated early senescence and photoinhibition. To elucidate the anthocyanin role, we monitored seasonal changes in photosynthetic traits, sugar, starch and N contents, pigment composition, and gene expression profiles in leaves exposed to substantially different light conditions
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Drought decreases cotton fiber strength by altering sucrose flow route J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 Honghai Zhu, Wei Hu, Yuxia Li, Jie Zou, Jiaqi He, Youhua Wang, Yali Meng, Binglin Chen, Wenqing Zhao, Shanshan Wang, Zhiguo Zhou
The potential mechanisms by which drought restricts cotton fiber cell wall synthesis and fiber strength formation are still not fully understood. Herein, drought experiments were conducted using two upland cotton cultivars of Dexiamian 1 (drought-tolerant) and Yuzaomian 9110 (drought-sensitive). Results showed that drought notably reduced sucrose efflux from cottonseed coats to fibers by down-regulating
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Non-photochemical quenching upregulation improves water use efficiency and reduces whole plant level water consumption under drought J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 Benjamin Turc, Seema Sahay, Jared Haupt, Talles de Oliveira Santos, Geng Bai, Katarzyna Glowacka
For crop production, the water supply limitations will likely become a bigger restriction underlining a need for crops that use less water per mass of production. Therefore, water use efficiency becomes a constraint in obtaining resilient and productive crops. We hypothesized that under drought conditions via modulation of chloroplast signal for stomatal opening by upregulation of non-photochemical
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Flowering time genes branching out J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 P E Colleoni, S W van Es, T Winkelmolen, R G H Immink, G W van Esse
Plants are sessile by nature, and as such they have evolved to sense changes in seasonality and their surrounding environment, and adapt to these changes. One prime example of this is the regulation of flowering time in angiosperms, which is precisely timed by the coordinative action of two proteins: FLOWERING LOCUS T (FT), and TERMINAL FLOWER 1 (TFL1). Both regulators are members of the PHOSPHATIDILETHANOLAMINE
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Plant ammonium sensitivity is associated with the external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 Mikel Rivero-Marcos, Berta Lasa, Tomé Neves, Ángel M Zamarreño, José M García-Mina, Carmen García-Olaverri, Pedro M Aparicio-Tejo, Cristina Cruz, Idoia Ariz
Modern crops exhibit diverse sensitivities to ammonium as the primary nitrogen source, influenced by environmental factors such as external pH and nutrient availability. Despite its significance, there is currently no systematic classification of plant species based on their ammonium sensitivity. This study conducts a meta-analysis of 50 plant species and presents a new classification method based
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AMPK activator 991 specifically activates SnRK1 and thereby affects seed germination in rice J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 Yuxiang Hu, Yan Lin, Jiaqi Bai, Xuemei Xu, Ziteng Wang, Chengqiang Ding, Yanfeng Ding, Lin Chen
Sucrose non-fermenting-1-related protein kinase 1 (SnRK1) and AMP-activated protein kinase (AMPK) are highly conserved. Compound 991 is an AMPK activator in mammals. However, whether 991 also activates SnRK1 remains unknown. The addition of 991 significantly increased SnRK1 activity in desalted extracts from germinating rice seeds in vitro. To determine whether 991 has biological activity, rice seeds
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Single-cell transcriptomics reveal heterogeneity in plant responses to the environment: a focus on biotic and abiotic interactions J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-11 Rubén Tenorio Berrío, Marieke Dubois
Environmental cues, from biotic or abiotic origin, are major factors influencing plant growth and productivity. Interactions with biotic (e.g. symbionts and pathogens) and abiotic (e.g. changes in temperature, water or nutrient availability) factors trigger signaling and downstream transcriptome changes in plants. While bulk RNA-sequencing technologies have traditionally been used to profile these
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Development of pollinated and unpollinated ovules in Ginkgo biloba: unravelling pollen’s role in ovule tissue maturation J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-09 Antonella Muto, Emanuela Talarico, Greta D’Apice, Maurizio Di Marzo, Silvia Moschin, Sebastiano Nigris, Nicola Babolin, Eleonora Greco, Fabrizio Araniti, Adriana Chiappetta, Lucia Colombo, Barbara Baldan, Leonardo Bruno
In gymnosperms such as Ginkgo biloba, the pollen's arrival plays a key role in ovule development, before fertilization occurs. Accordingly, G. biloba female plants geographically isolated from male plants aborted all their ovules after the pollination drop emission, which is the event that allows the ovule to capture pollen grains. To decipher the mechanism induced by pollination required to avoid
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The pathogenesis-related protein, PRP1, negatively regulates root nodule symbiosis in Lotus japonicus J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-08 Hao Li, Yajuan Ou, Kui Huang, Zhongming Zhang, Yangrong Cao, Hui Zhu
The legume-rhizobium symbiosis represents as a unique model within the realm of plant-microbe interactions. Unlike typical cases of pathogenic invasion, the infection of rhizobia and their residence within symbiotic cells do not elicit a noticeable immune response in plants. Nevertheless, there is still much to uncover regarding the mechanisms through which plant immunity influences rhizobia symbiosis
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Superoxide signalling and antioxidant processing in the plant nucleus J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-08 Barbara Karpinska, Christine H Foyer
The superoxide anion radical (O2•−) is a one-electron reduction product of molecular oxygen. Compared to other forms of reactive oxygen species (ROS), superoxide has limited reactivity. Nevertheless, superoxide reacts with nitic oxide, ascorbate and the iron moieties of [Fe–S] cluster-containing proteins. Superoxide has largely been neglected as a signalling molecule in the plant literature in favour
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Genetic and epigenetic basis of phytohormones control of floral transition in plants J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-08 Xiaoxiao Li, Chuyu Lin, Chenghao Lan, Zeng Tao
The timing of the developmental transition from the vegetative to the reproductive stages is critical for angiosperm and fine-tuned by the integration of endogenous factors and external environmental cues to ensure proper and successful reproduction. Plants have evolved sophisticated mechanisms to response to diverse environmental or stress signals, which may be mediated by plant hormones which coordinate
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ZmMAPK6, a mitogenactivated protein kinase, regulates maize kernel weight J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-07 Wenyu Li, Yayong Li, Huiyue Shi, Han Wang, Kun Ji, Long Zhang, Yan Wang, Yongbin Dong, Yuling Li
Kernel weight is a critical agronomic trait in maize production. Many genes are related to it, but only a few of them have been applied to maize breeding and cultivation. Here, we identify a novel function of maize mitogenactivated protein kinase 6 (ZmMPK6) in the regulation of maize kernel weight. The kernel weight reduced in zmmpk6 mutants, while enhanced in ZmMPK6 overexpression lines. In addition
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RhMYB17 regulates the homeotic transformation of floral organs in rose (Rosa hybrida) under cold stress J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-07 Tuo Yang, Yi Wang, Yuqi Li, Shangyi Liang, Yunyao Yang, Ziwei Huang, Yonghong Li, Junping Gao, Nan Ma, Xiaofeng Zhou
Low temperatures affect flower development in rose (Rosa hybrida), increasing petaloid stamen number and reducing normal stamen number. We identified the low-temperature-responsive R2R3-MYB transcription factor RhMYB17, which is homologous to Arabidopsis (Arabidopsis thaliana) MYB17. RhMYB17 was upregulated at low temperatures, and RhMYB17 transcripts accumulated in floral buds. Transient silencing
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Evolutionary insights into strategy shifts for the safe and effective accumulation of ascorbate in plants J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-06 Takanori Maruta, Yasuhiro Tanaka, Kojiro Yamamoto, Tetsuya Ishida, Akane Hamada, Takahiro Ishikawa
Plants accumulate high concentrations of ascorbate, commonly in their leaves, as a redox buffer. While ascorbate levels have increased during plant evolution, the mechanisms behind this phenomenon are unclear. Moreover, has the increase in ascorbate concentration been achieved without imposing any detrimental effects on the plants? In this review, we focus on potential transitions in two regulatory
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Application of the thermal death time model in predicting thermal damage accumulation in plants J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-06 Andreas H Faber, Michael Ørsted, Bodil Kirstine Ehlers
The thermal death time (TDT) model suggests that the duration an organism can tolerate thermal stress decreases exponentially as the intensity of the temperature becomes more extreme. This model has been used to predict damage accumulation in ectothermic animals and plants under fluctuating thermal conditions. However, the critical assumption of the TDT model, which is additive damage accumulation
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Stress response membrane protein gene OsSMP2 negatively regulates rice tolerance to drought J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-06 Wei Wang, Yaqi Li, Songjin Yang, Jiacheng Wu, Chuan Ma, Yulin Chen, Xingzhuo Sun, Lingli Wu, Xin Liang, Qiuping Fu, Zhengjun Xu, Lihua Li, Zhengjian Huang, Jianqing Zhu, Xiaomei Jia, Xiaoying Ye, Rongjun Chen
In our gene chip analysis, OsSMP2 gene expression was induced under various abiotic stresses, prompting an investigation into its role in drought resistance and ABA signaling. Subsequent experiments, including qRT-PCR and GUS activity detection, affirmed the OsSMP2 gene's predominant induction by drought stress. Subcellular localization experiments indicated the OsSMP2 protein primarily localizes to
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FT-mediated thermal signalling regulates age-dependent inflorescence development in Arabidopsis thaliana J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Pablo González-Suárez, Catriona H Walker, Thomas Lock, Tom Bennett
Many plants show strong heteroblastic changes in the shape and size of organs as they transition from juvenile to reproductive age. Most attention has been focussed on heteroblastic development on leaves, but we wanted to understand heteroblastic changes in reproductive organ size. We therefore studied the progression of reproductive development in the model plant Arabidopsis thaliana, and found strong
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Illuminating stomatal responses to red light: establishing the role of C i-dependent vs -independent mechanisms in control of stomatal behaviour J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Georgia Taylor, Julia Walter, Johannes Kromdijk
The stomatal response to red light appears to link stomatal conductance (gs) with photosynthetic rates. Initially, it was suggested that changes in intercellular CO2 (Ci) provide the main cue via a Ci-dependent response. However, evidence for Ci-independent mechanisms suggests an additional, more direct relationship with photosynthesis. While both Ci-dependent and -independent mechanisms clearly function
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bHLH121 and clade IVc bHLH transcription factors synergistically function to regulate iron homeostasis in Arabidopsis thaliana J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Fei Gao, Meijie Li, Christian Dubos
Iron is an essential micronutrient for plant growth and development. In Arabidopsis thaliana, an intricate regulatory network involving several bHLH transcription factors (TFs) controls the homeostasis of iron. Among these TFs, bHLH121 plays a crucial role. bHLH121 interacts in vivo with clade IVc bHLH TFs and activates the expression of FIT and clade Ib bHLH TFs to stimulate the uptake of iron. How
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Function of the ascorbate peroxidase (APX) in fruits and their modulation by reactive species J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Francisco J Corpas, Salvador González-Gordo, José M Palma
Ascorbate peroxidase (APX) is one of the enzymes of the ascorbate-glutathione cycle and is the key enzyme that breaks down H2O2 with the aid of ascorbate as an electron source, although other enzymes also break down H2O2 such as catalase, peroxiredoxins, among others. APX is present in all photosynthetic eukaryotes from algae to higher plants and at the cellular level, it is localized in all subcellular
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Unlocking protein-protein interactions in plants: A comprehensive review of established and emerging techniques J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Alvaro Furones Cuadrado, Daniël Van Damme
Protein-protein interactions orchestrate plant development and serve as crucial elements for cellular and environmental communication. Understanding these interactions offers a gateway to unravel complex protein networks that will allow a better understanding of nature. Methods for the characterization of protein-protein interactions have been around for a long time, yet the complexity of some of these
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Short-term salt stress reduces photosynthetic oscillations under triose phosphate utilization limitation in tomato J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Yuqi Zhang, Elias Kaiser, Satadal Dutta, Thomas D Sharkey, Leo F M Marcelis, Tao Li
Triose phosphate utilization (TPU) limitation is one of the three biochemical limitations of photosynthetic CO2 assimilation rate in C3 plants. Under TPU limitation, abrupt and large transitions in light intensity cause damped oscillations in photosynthesis. When plants are salt-stressed, photosynthesis is often down-regulated particularly under dynamic light intensity, but how salt stress affects
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Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Rosalie Sinclair, Minmin Wang, Muhammad Zaki Jawaid, Toshisangba Longkumer, Jesse Aaron, Blair Rossetti, Eric Wait, Kent McDonald, Daniel Cox, John Heddleston, Thomas Wilkop, Georgia Drakakaki
Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodeling, and timely polysaccharide deposition, such as callose. Understanding such a dynamic process requires dissection in time and space; this has been a major hurdle in studying cytokinesis. Using lattice light sheet microscopy (LLSM) we studied cell plate
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Optimizing nutrient transporters to enhance disease resistance in rice: a review J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-04 Shugang Hui, Peng Zhang, Meng Yuan
Fertilizers and plant diseases contribute positively and negatively to crop production, respectively. Macronutrients and micronutrients provided by the soil and fertilizers are transported by various plant nutrient transporters from the soil to plant roots or shoots, facilitating plants growth and development. However, the homeostasis of different nutrients has diverse effects on plant disease. The
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Hi-C techniques: from genome assemblies to transcription regulation J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-02 Hana Šimková, Amanda Souza Câmara, Martin Mascher
The invention of chromosome-conformation capture (3C) techniques, in particular the key method Hi-C providing genome-wide information about chromatin contacts, revolutionized the way we study the three-dimensional (3D) organization of the nuclear genome and how it impacts transcription, replication and DNA repair. Since the frequency of chromatin contacts between pairs of genomic segments predictably
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Root topological order drives variation of fine root vessel traits and hydraulic strategies in tropical trees J. Exp. Bot. (IF 6.9) Pub Date : 2024-03-01 Guangqi Zhang, Claire Fortunel, Shan Niu, Juan Zuo, Jean-Luc Maeght, Xiaodong Yang, Shangwen Xia, Zhun Mao
Vessel traits contribute to plant water transport from roots to leaves and thereby influence how plants respond to soil water availability, but the sources of variation in fine roots anatomical traits remain poorly understood. Here, we explore the variations of fine root vessel traits along topological orders within and across tropical tree species. Anatomical traits were measured along five root topological
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Chromatin sensing: Integration of environmental signals to reprogram plant development through chromatin regulators J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-29 Wenli Wang, Sibum Sung
Chromatin regulation in eukaryotes plays pivotal roles in controlling developmental regulatory gene network. This review explores the intricate interplay between chromatin regulators and environmental signals, elucidating their roles in shaping plant development. As sessile organisms, plants have evolved sophisticated mechanisms to perceive and respond to environmental cues, orchestrating developmental
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A novel workflow for unbiased quantification of autophagosomes in 3D in Arabidopsis thaliana roots J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-29 Michal Daněk, Daniela Kocourková, Tereza Korec Podmanická, Kateřina Eliášová, Kristýna Nesvadbová, Pavel Krupař, Jan Martinec
Macroautophagy is often quantified by live imaging of autophagosomes labeled with fluorescently tagged ATG8 protein (FP-ATG8) in Arabidopsis thaliana. The labeled particles are then counted in single focal planes. This approach may lead to inaccurate results as the actual 3D distribution of autophagosomes is not taken into account and appropriate sampling in the Z-direction is not performed. To overcome
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The chromosome-scale genome of wild Brassica oleracea provides insights into the domestication of Brassica plants J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-29 Gaoxiang Ji, Ying Long, Guangqin Cai, Aihua Wang, Guixin Yan, Hao Li, Guizhen Gao, Kun Xu, Qian Huang, Biyun Chen, Lixia Li, Feng Li, Takeshi Nishio, Jinxiong Shen, Xiaoming Wu
The cultivated diploid Brassica oleracea is an important vegetable crop, but the genetic basis of domestication remains largely unclear without high-quality reference genomes of wild B. oleracea. Here, we report the first chromosome-level assembly of the wild Brassica oleracea L. W03 genome, (total genome size, 630.7 Mb; scaffold N50, 64.6 Mb). Using newly assembled W03 genome, we constructed a gene-based
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Why leaves become isotopically lighter than photosynthetic carbon isotope discrimination explains: on the importance of post-photosynthetic fractionation J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-28 Arthur Gessler
This article comments on: Yu YZ, Liu HT, Yang F, Li L, Schäufele R, Tcherkez G, Schnyder H, Gong XY. 2024. δ13C of bulk organic matter and cellulose reveal post-photosynthetic fractionation during ontogeny in C4 grass leaves. Journal of Experimental Botany 75, 1451–1464
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Spatial regulation of immunity: unmasking the secrets of abaxial immunity to powdery mildew J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-28 Dawei Xu, Li Yang
This article comments on: Wu Y, Sexton WK, Zhang Q, Bloodgood D, Wu Y, Hooks C, Coker F, Vasquez A, Wei C-I, Xiao S. 2024. Leaf abaxial immunity to powdery mildew in Arabidopsis is conferred by multiple defense mechanisms. Journal of Experimental Botany 75, 1465–1478.
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Waking up Sleeping Beauty: DNA damage activates dormant stem cell division by enhancing brassinosteroid signaling J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-28 Jefri Heyman, Lieven De Veylder
This article comments on: Takahashi N, Suita K, Koike T, Ogita N, Zhang Y, Umeda M. 2024. DNA double-strand breaks enhance brassinosteroid signaling to activate quiescent center cell division in Arabidopsis. Journal of Experimental Botany 75, 1364–1375.
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Combined leaf gas-exchange system for model assessment J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-27 Jun Tominaga, Yoshinobu Kawamitsu
Leaf gas-exchange measurements are useful in assessing environmental responses of plants. However, uncertainties in the leaf gas-exchange model potentially limit its application. The main challenge in the model-dependent calculations is to detect violations of assumptions. Here, we developed a system that integrates the direct measurement of leaf intercellular CO2 concentration and the standard open-flow
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Evidence for dual targeting control of Arabidopsis 6-phosphogluconate dehydrogenase isoforms by N-terminal phosphorylation J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-27 Lennart Nico Doering, Niklas Gerling, Loreen Linnenbrügger, Hannes Lansing, Marie-Christin Baune, Kerstin Fischer, Antje von Schaewen
The oxidative pentose-phosphate pathway (OPPP) retrieves NADPH from glucose-6-phosphate, which is important in chloroplasts at night and in plastids of heterotrophic tissues. We previously studied how OPPP enzymes may transiently locate to peroxisomes, but how this is achieved for the 3rd enzyme remained unclear. By extending our genetic approach, we could demonstrate that Arabidopsis isoform 6-phosphogluconate
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The transcription factor ThDof8 binds to a novel cis-element and mediates molecular response to salt stress in Tamarix hispida J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-24 Peilong Wang, Danni Wang, Yongxi Li, Jinghang Li, Baichao Liu, Yuanyuan Wang, Caiqiu Gao
Salt stress is a commom abiotic factor that restricts plant growth and development. As a halophyte plant, it is of great significance to explore the salt tolerance genes and regulatory mechanisms of Tamarix hispida. Dof (DNA-bining with one finger), as an important transcription factor (TF), influences and controls various signaling substances involved in diverse biological processes related to plant
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Excessive iron deposition in root apoplast is involved in growth arrest of roots in response to proton stress J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-24 Xian Zhi Fang, Xiao Lan Xu, Zheng Qian Ye, Dan Liu, Ke Li Zhao, Dong Ming Li, Xing Xing Liu, Chong Wei Jin
The rhizotoxicity of protons (H+) in acidic soils is a fundamental constraint that results in serious yield losses. However, the mechanisms underlying H+-mediated inhibition of root growth are poorly understood. In this study, we revealed that H+-induced root growth inhibition depends considerably on excessive iron deposition in root apoplasts. Reducing such aberrant iron deposition by decreasing the
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Transmembrane formins as active cargoes of membrane trafficking J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-24 Fatima Cvrčková, Rajdeep Ghosh, Helena Kočová
Formins are a large, evolutionarily old family of plant cytoskeletal regulators whose roles include actin capping and nucleation, as well as modulation of microtubule dynamics. The plant class I formin clade is characterized by a unique domain organization, as most of its members are transmembrane proteins with possible cell wall-binding motifs exposed to the extracytoplasmic space – a structure that
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Root endophyte-mediated alteration in plant H2O2 homeostasis regulates symbiosis outcome and reshapes the rhizosphere microbiota J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-23 Kai Sun, Yi-Tong Pan, Hui-Jun Jiang, Jia-Yan Xu, Chen-Yu Ma, Jiayu Zhou, Yunqi Liu, Sergey Shabala, Wei Zhang, Chuan-Chao Dai
Endophytic symbioses between plants and fungi are a dominant feature of many terrestrial ecosystems, yet little is known about the signaling that defines these symbiotic associations. Hydrogen peroxide (H2O2) is recognized as a key signal mediating the plant adaptive response to both biotic and abiotic stresses. However, the role of H2O2 in plant-fungal symbiosis remains elusive. Using a combination
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ZmNF-YC1-ZmAPRG pathway modulates low phosphorus tolerance in maize J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-23 Yang Bai, Qiuyue Yang, Yuling Gan, Mei Li, Zikun Zhao, Erfei Dong, Chaofeng Li, Di He, Xiupeng Mei, Yilin Cai
Phosphorus (P) is an essential nutrient for plant growth and yield. Low phosphate-use-efficiency makes it important to clarify the molecular mechanism of low phosphorus stress. In our previous studies, a phosphorus efficiency gene ZmAPRG was identified. Here, we further screened the upstream regulator ZmNF-YC1 of ZmAPRG by yeast one hybrid (Y1H) assay, and found it was a low Pi-inducible gene. The
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Chloroplast thiol redox dynamics through the lens of genetically encoded biosensors J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-22 Stefanie J Müller-Schüssele
Chloroplasts fix carbon by using light energy and have evolved a complex redox network that supports plastid functions by protection against ROS as well as by metabolic regulation according to environmental conditions. In thioredoxin- and glutathione/glutaredoxin-dependent redox cascades, protein cysteinyl redox steady states are set by varying oxidation and reduction rates. The specificity and interplay
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Untranslated yet indispensable – UTRs act as key regulators in the environmental control of gene expression J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-22 Emma C Hardy, Martin Balcerowicz
To survive and thrive in a dynamic environment, plants must continuously monitor their surroundings and adjust their development and physiology accordingly. Changes in gene expression underlie these developmental and physiological adjustments and are traditionally attributed to widespread transcriptional reprogramming. Growing evidence, however, suggests that post-transcriptional mechanisms also play
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Non-foliar photosynthesis and nitrogen assimilation influence grain yield in durum wheat regardless of water conditions J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-22 Rubén Vicente, Omar Vergara-Díaz, Estefanía Uberegui, Raquel Martínez-Peña, Rosa Morcuende, Shawn C Kefauver, Camilo López-Cristoffanini, Nieves Aparicio, María Dolores Serret, José Luis Araus
There is a need to generate improved crop varieties adapted to the ongoing changes in the climate. We studied durum wheat canopy and central metabolism of six different photosynthetic organs in two yield-contrasting varieties. The aim was to understand the mechanisms associated with the water stress response and yield performance. Water stress strongly reduced grain yield, plant biomass and leaf photosynthesis
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Characterization of the cryptic interspecific hybrid Lemna × mediterranea by an integrated approach provides new insights into duckweed diversity J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-22 Luca Braglia, Simona Ceschin, M Adelaide Iannelli, Manuela Bog, Marco Fabriani, Giovanna Frugis, Floriana Gavazzi, Silvia Gianì, Flaminia Mariani, Maurizio Muzzi, Emanuele Pelella, Laura Morello
Lemnaceae taxonomy is challenged by the particular morphology of these tiny free-floating angiosperms. Although molecular taxonomy has helped clarify the phylogenetic history of this family, some inconsistency with morphological data leads to frequent misclassifications in the genus Lemna. Recently, the finding that Lemna japonica is an interspecific hybrid between Lemna minor and Lemna turionifera
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Hyperspectral imaging reveals small-scale water gradients in apple leaves due to minimal cuticle perforation by Venturia inaequalis conidiophores J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-22 Erich-Christian Oerke, Ulrike Steiner
Effects of Venturia inaequalis on water relations of apple leaves were studied under controlled conditions without limitation of water supply to elucidate its impact on the non-haustorial biotrophy of this pathogen. Leaf water relations – leaf water content, transpiration - were spatially resolved by hyperspectral imaging and thermography; non-imaging techniques - gravimetry, pressure chamber, porometer
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The maternal embrace: the protection of plant embryos J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-21 Sjoerd Woudenberg, Feras Hadid, Dolf Weijers, Cecilia Borassi
All land plants - the embryophytes - produce multicellular embryos, as other multicellular organisms, such as brown algae and animals. A unique characteristic of plant embryos is their immobile and confined nature. Their embedding in maternal tissues may offer protection from the environment, but also physically constrains development. Across the different land plants, a huge discrepancy is present
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Twenty years of irrigation acclimation is driven by denser canopies and not by plasticity in twig- and needle-level hydraulics in a Pinus sylvestris forest J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-20 Alice Gauthey, Christoph Bachofen, Alana Chin, Hervé Cochard, Jonas Gisler, Eugénie Mas, Katrin Meusburger, Richard L Peters, Marcus Schaub, Alex Tunas, Roman Zweifel, Charlotte Grossiord
Climate change is predicted to increase atmospheric vapor pressure deficit, exacerbating soil drought, and thus enhancing tree evaporative demand and mortality. Yet, few studies have addressed the longer-term drought acclimation strategy of trees, particularly the importance of morphological vs. hydraulic plasticity. Using a long-term (20 years) irrigation experiment in a natural forest, we investigated
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Seed traits and recruitment interact with habitats to generate patterns of local adaptation in a perennial grass J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-20 Samsad Razzaque, Thomas E Juenger
A fundamental challenge in the field of ecology involves understanding the adaptive traits and life history stages regulating the population dynamics of species across diverse habitats. Seed traits and early seedling vigor are thought to be key functional traits in plants with important consequences for recruitment, establishment, and population persistence. However, little is known about how diverse
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Epigenetics and plant hormone dynamics - a functional and methodological perspective J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-19 Jiří Rudolf, Lucia Tomovičová, Klára Panzarová, Jiří Fajkus, Jan Hejátko, Jan Skalák
Plant hormones, pivotal regulators of plant growth, development, and response to environmental cues, have recently emerged as central modulators of epigenetic processes governing gene expression and phenotypic plasticity. This review addresses the complex interplay between plant hormones and epigenetic mechanisms, highlighting the diverse methodologies that have been harnessed to decipher these intricate
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The Circadian Clock And Thermal Regulation In Plants: Novel Findings On The Role Of Positive Circadian Clock-Regulators In Temperature Responses J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-19 María José de Leone, Marcelo Javier Yanovsky
The impact of rising global temperatures on crop yields is a serious concern, and developing heat-resistant crop varieties is crucial for mitigating the effects of climate change on agriculture. To achieve this, a better understanding of the molecular basis of the thermal responses in plants is necessary. The circadian clock plays a central role in modulating plant biology in synchrony with environmental
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Prime Editing in Plants: Prospects and Challenges J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-17 Sanskriti Vats, Jitesh Kumar, Humira Sonah, Feng Zhang, Rupesh Deshmukh
Prime editors are reverse transcriptase (RT)-based genome editing tools utilizing double strand break (DSB) free mechanisms to decrease off-target editing in genomes and enhance the efficiency of targeted insertions. Multiple prime editors developed within a short span of time are a testament to the potential of this technique for targeted insertions. This is mainly because of the possibility of generation
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Revisiting the role of ascorbate oxidase in plant systems J. Exp. Bot. (IF 6.9) Pub Date : 2024-02-17 Ifigeneia Mellidou, Angelos K Kanellis
Ascorbic acid (AsA) plays an indispensable role in plants, serving as both an antioxidant and a master regulator of the cellular redox balance. Ascorbate oxidase (AO) is a blue copper oxidase, responsible for the oxidation of AsA with the simultaneous production of water. For many decades, AO was erroneously postulated as an enzyme without any obvious advantage, as it decreases the AsA pool size and