-
The Catabolic Network of Aromatoleum aromaticum EbN1T. Microb. Physiol. (IF 1.2) Pub Date : 2023-10-10 Patrick Becker,Daniel Wünsch,Lars Wöhlbrand,Meina Neumann-Schaal,Dietmar Schomburg,Ralf Rabus
The denitrifying betaproteobacterium Aromatoleum aromaticum EbN1T is a facultative anaerobic degradation specialist and belongs to the environmental bacteria studied best on the proteogenomic level. This review summarizes the current state of knowledge about the anaerobic and aerobic degradation (to CO2) of 47 organic growth substrates (23 aromatic, 21 aliphatic, and 3 amino acids) as well as the modes
-
Targeted cell labeling and sorting of Prokaryotes for cultivation and omics approaches. Microb. Physiol. (IF 1.2) Pub Date : 2023-09-30 Gunnar Sturm,Mohammad Mojarrad,Anne-Kristin Kaster
To date, the vast majority of prokaryotic organisms escapes detailed characterization because they cannot be isolated in axenic cultures. These organisms are referred to as microbial dark matter (MDM). Targeted labelling and sorting of these microorganisms paves the way for single-cell, enrichment or cultivation approaches. In this review, we describe an array of different methods ranging from labeling-free
-
Sequence Similarity Among Structural Repeats in the Piezo Family of Mechanosensitive Ion channels. Microb. Physiol. (IF 1.2) Pub Date : 2023-06-15 Kevin J Hendargo,Ashay O Patel,Onyeka S Chukwudozie,Gabriel Moreno-Hagelsieb,J Andres Christen,Arturo Medrano-Soto,Milton H Saier
Members of the Piezo family of mechanically activated cation channels are involved in multiple physiological processes including vascular development, cell differentiation, touch perception, hearing and more. Mutations in these proteins are associated with a variety of diseases, such as colorectal adenomatous polyposis, dehydrated hereditary stomacytosis, and hereditary xerocytosis. Available 3D structures
-
Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-forming Capabilities of Soil Lignocellulolytic Bacteria Microb. Physiol. (IF 1.2) Pub Date : 2023-03-21 Aristide Laurel Mokale Kognou, Chonlong Chio, Janak Raj Khatiwada, Sarita Shrestha, Xuantong Chen, Yuen Zhu, Rosalie Anne Ngono Ngane, Gabriel Agbor Agbor, Zi-Hua Jiang, Chunbao Charles Xu, Wensheng Qin
Soil bacteria participate in self-immobilization processes for survival, persistence and producing virulence factors in some niches or hosts through their capacities of autoaggregation, cell surface hydrophobicity, biofilm formation, and antibiotic and heavy metal resistance. This study investigated potential virulence, antibiotics and heavy metals resistance, solvent adhesion, and biofilm-forming
-
Identification of a Novel LysR Family Transcriptional Regulator Controlling Acquisition of Sulfur Sources in Acinetobacter baumannii Microb. Physiol. (IF 1.2) Pub Date : 2023-01-10 Alaska Pokhrel, Hue Dinh, Liping Li, Karl A. Hassan, Amy K. Cain, Ian T. Paulsen
l-cysteine biosynthesis from inorganic sulfur represents a major mechanism by which reduced sulfur is incorporated into organic compounds. Cysteine biosynthesis and regulation is well characterized in Escherichia coli. However, the regulation of sulfur metabolism in Acinetobacter baumannii is only partly understood, with the LysR-type regulator, GigC known to control some aspects of sulfur reduction
-
Optimization of Cultural Conditions for Pectinase Production by Streptomyces sp. and Characterization of Partially Purified Enzymes Microb. Physiol. (IF 1.2) Pub Date : 2022-11-23 Sarita Shrestha, Chonlong Chio, Janak Raj Khatiwada, Aristide Laurel Mokale Kognou, Xuantong Chen, Wensheng Qin
The cultural parameters of Streptomyces sp. for pectinase production were optimized using the Box-Behnken design. The maximum pectinase production was obtained after 58 h at 35°C and pH 7 upon submerged fermentation in yeast extract-containing media. The enzymes were partially purified with acetone precipitation, and the analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram
-
Heterogeneity of Subcellular Diffusion in Bacteria Based on Spatial Segregation of Ribosomes and Nucleoids Microb. Physiol. (IF 1.2) Pub Date : 2022-09-07 Simon Dersch, Daniel A.O. Rotter, Peter L. Graumann
It has long become clear that in spite of generally lacking internal membrane systems, bacteria contain well-structured subcellular structures of usually filamentous proteins, and a preferred 3D arrangement of their chromosome(s). Some of these systems are set up by so-called cytoskeletal elements, or by polar landmark proteins, but the mechanism of specific localization is still unclear in most cases
-
Polyphosphate Kinases Phosphorylate Thiamine Phosphates Microb. Physiol. (IF 1.2) Pub Date : 2022-08-30 Jennie C. Hildenbrand, Georg A. Sprenger, Attila Teleki, Ralf Takors, Dieter Jendrossek
Polyphosphate kinases (PPKs) catalyze the reversible transfer of the γ-phosphate moiety of ATP (or of another nucleoside triphosphate) to a growing chain of polyphosphate (polyP). In this study, we describe that PPKs of various sources are additionally able to phosphorylate thiamine diphosphate (ThP2) to produce thiamine triphosphate (ThP3) and even thiamine tetraphosphate in vitro using polyP as phosphate
-
Physiological Robustness of Model Gram-Negative Bacteria in Response to Genome Rewiring Microb. Physiol. (IF 1.2) Pub Date : 2022-08-30 Charles J. Dorman, Matthew J. Dorman
DNA supercoiling and nucleoid-associated proteins (NAPs) are two of the factors that govern the architecture of the bacterial genome, influencing the expression of the genetic information that it contains. Alterations to DNA topology, and to the numbers and types of NAPs, have pleiotropic effects on gene expression, suggesting that modifications to the production patterns of DNA topoisomerases and/or
-
Eating Animal Products, a Common Cause of Human Diseases Microb. Physiol. (IF 1.2) Pub Date : 2022-08-11 Milton H. Saier Jr., Stephen M. Baird, B. Lakshmi Reddy, Peter W. Kopkowski
The human population is plagued by hundreds of infectious agents that cause diseases, and many of these agents can infect a range of wild and domesticated animals as well. In fact, a large proportion of current pathological conditions in humans is caused by our close association with nonhuman animals, some of which we keep as pets, but most of which we raise, prepare as food sources, and ingest. It
-
Nanomolar Responsiveness of Marine Phaeobacter inhibens DSM 17395 toward Carbohydrates and Amino Acids Microb. Physiol. (IF 1.2) Pub Date : 2022-04-25 Arne Weiten, Kristin Kalvelage, Meina Neumann-Schaal, Ramona Buschen, Sabine Scheve, Michael Winklhofer, Ralf Rabus
Phaeobacter inhibens DSM 17395 is a heterotrophic member of the ubiquitous, marine Roseobacter group and specializes in the aerobic utilization of carbohydrates and amino acids via pathways widespread among roseobacters. The in vivo responsiveness of P. inhibens DSM 17395 was studied with nonadapted cells (succinate-grown), which were exposed to a single pulse (100–0.01 µM) each of N-acetylglucosamine
-
Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel Microb. Physiol. (IF 1.2) Pub Date : 2022-04-07 Michelle Kammel, Oliver Trebbin, Robert Gary Sawers
The formate channel A (FocA) belongs to the formate-nitrite transporter (FNT) family, members of which permeate small monovalent anions. FocA from Escherichia coli translocates formate/formic acid bi-directionally across the cytoplasmic membrane during fermentative growth. Two residues are particularly well-conserved within the translocation pores of FNTs: threonine-91 and histidine-209, based on E
-
Luciferase-Based Determination of ATP/NAD(H) Pools in a Marine (Environmental) Bacterium. Microb. Physiol. (IF 1.2) Pub Date : 2022-03-25 Daniel Wünsch,Sabine Scheve,Arne Weiten,Kristin Kalvelage,Ralf Rabus
In all living organisms, adenosine triphosphate (ATP) and NAD(H) represent universal molecular currencies for energy and redox state, respectively, and are thus widely applicable molecular proxies for an organism's viability and activity. To this end, corresponding luciferase-based assays in combination with a microplate reader were established with the marine model bacterium Phaeobacter inhibens DSM
-
Assembly of Bacillus subtilis Dynamin into Membrane-Protective Structures in Response to Environmental Stress Is Mediated by Moderate Changes in Dynamics at a Single Molecule Level. Microb. Physiol. (IF 1.2) Pub Date : 2022-03-10 Laura Sattler,Peter L Graumann
Dynamin-like proteins are membrane-associated GTPases, conserved in bacteria and in eukaryotes, that can mediate nucleotide-driven membrane deformation or membrane fusion reactions. Bacillus subtilis' DynA has been shown to play an important role in protecting cells against chemicals that induce membrane leakage, and to form an increased number of membrane-associated structures after induction of membrane
-
Migration of Polyphosphate Granules in Agrobacterium tumefaciens. Microb. Physiol. (IF 1.2) Pub Date : 2022-02-15 Celina Frank,Daniel Pfeiffer,Meriyem Aktas,Dieter Jendrossek
Agrobacterium tumefaciens has two polyphosphate (polyP) kinases, one of which (PPK1AT) is responsible for the formation of polyP granules, while the other (PPK2AT) is used for replenishing the NTP pools by using polyP as a phosphate donor to phosphorylate nucleoside diphosphates. Fusions of eYFP with PPK2AT or of the polyP granule-associated phosin PptA from Ralstonia eutropha always co-localized with
-
Discovery and Characterization of the Phospholemman/SIMP/Viroporin Superfamily. Microb. Physiol. (IF 1.2) Pub Date : 2022-02-11 Daniel Tyler,Kevin J Hendargo,Arturo Medrano-Soto,Milton H Saier
Using bioinformatic approaches, we present evidence of distant relatedness among the Ephemerovirus Viroporin family, the Rhabdoviridae Putative Viroporin U5 family, the Phospholemman family, and the Small Integral Membrane Protein family. Our approach is based on the transitivity property of homology complemented with five validation criteria: (1) significant sequence similarity and alignment coverage
-
The Genome Reduction Excludes the Ribosomal Rescue System in Acholeplasmataceae. Microb. Physiol. (IF 1.2) Pub Date : 2022-01-31 Christina Zübert,Anna-Marie Ilic,Bojan Duduk,Michael Kube
The trans-translation process is a ribosomal rescue system for stalled ribosomes processing truncated mRNA. The genes ssrA and smpB fulfil the key functions in most bacteria, but some species have either lost these genes or the function of the ribosomal rescue system is taken over by other genes. To date, the ribosomal rescue system has not been analysed in detail for the Acholeplasmataceae. This family
-
Comparative Analyses of the Transport Proteins Encoded within the Genomes of nine Bifidobacterium Species. Microb. Physiol. (IF 1.2) Pub Date : 2021-09-23 Hassan Zafar,Milton H Saier
The human microbiome influences human health in both negative and positive ways. Studies on the transportomes of these organisms yield information that may be utilized for various purposes, including the identification of novel drug targets and the manufacture of improved probiotic strains. Moreover, these genomic analyses help to improve our understanding of the physiology and metabolic capabilities
-
Colonization and Development of the Fecal Microflora of South China Tiger Cubs (Panthera tigris amoyensis) by Sequencing of the 16S rRNA Gene. Microb. Physiol. (IF 1.2) Pub Date : 2021-09-14 Yanfa Sun,Jie Yao,Min Zhang,Tengteng Chen,Weihua Xu,Wenyuan Fu,Qiong Wu,Yan Li,Xingxing Chen,Yuting Zhu,Xuemei Zhang,Lingyu Liu,Donghong Chen,Zhenyuan Wang,Zhangjing You,Xuebing Zhang,Yi Liu,Kaixiong Lin,Weiming Lin
Postnatal colonization and development of the gut microbiota is linked to health and growth. A comprehensive understanding of the postnatal compositional changes and development of the microbial community is helpful to understand the gut health and improve the survival rate of South China tiger cubs (Panthera tigris amoyensis). Fecal samples from three tiger cubs were collected on the day of birth
-
The Formation of Spore-Like Akinetes: A Survival Strategy of Filamentous Cyanobacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-09-03 Ritu Garg,Iris Maldener
Some cyanobacteria of the order Nostocales can form akinetes, spore-like dormant cells resistant to various unfavorable environmental fluctuations. Akinetes are larger than vegetative cells and contain large quantities of reserve products, mainly glycogen and the nitrogen storage polypeptide polymer cyanophycin. Akinetes are enveloped in a thick protective coat containing a multilayered structure and
-
Contribution of the Clp Protease to Bacterial Survival and Mitochondrial Homoeostasis. Microb. Physiol. (IF 1.2) Pub Date : 2021-08-26 Astrid Illigmann,Yvonne Thoma,Stefan Pan,Laura Reinhardt,Heike Brötz-Oesterhelt
Fast adaptation to environmental changes ensures bacterial survival, and proteolysis represents a key cellular process in adaptation. The Clp protease system is a multi-component machinery responsible for protein homoeostasis, protein quality control, and targeted proteolysis of transcriptional regulators in prokaryotic cells and prokaryote-derived organelles of eukaryotic cells. A functional Clp protease
-
Secondary Metabolites Governing Microbiome Interaction of Staphylococcal Pathogens and Commensals. Microb. Physiol. (IF 1.2) Pub Date : 2021-07-29 Benjamin O Torres Salazar,Simon Heilbronner,Andreas Peschel,Bernhard Krismer
Various Staphylococcus species colonize skin and upper airways of warm-blooded animals. They compete successfully with many other microorganisms under the hostile and nutrient-poor conditions of these habitats using mechanisms that we are only beginning to appreciate. Small-molecule mediators, whose biosynthesis requires complex enzymatic cascades, so-called secondary metabolites, have emerged as crucial
-
A Novel Enrichment Culture Highlights Core Features of Microbial Networks Contributing to Autotrophic Fe(II) Oxidation Coupled to Nitrate Reduction. Microb. Physiol. (IF 1.2) Pub Date : 2021-07-02 Yu-Ming Huang,Daniel Straub,Andreas Kappler,Nicole Smith,Nia Blackwell,Sara Kleindienst
Fe(II) oxidation coupled to nitrate reduction (NRFO) has been described for many environments. Yet very few autotrophic microorganisms catalysing NRFO have been cultivated and their diversity, as well as their mechanisms for NRFO in situ remain unclear. A novel autotrophic NRFO enrichment culture, named culture BP, was obtained from freshwater sediment. After more than 20 transfers, culture BP oxidized
-
Editorial for Article Collection on "Bacterial Survival Strategies". Microb. Physiol. (IF 1.2) Pub Date : 2021-07-01 Karl Forchhammer
-
CRISPR-Cas System: The Powerful Modulator of Accessory Genomes in Prokaryotes. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-30 Anca Butiuc-Keul,Anca Farkas,Rahela Carpa,Dumitrana Iordache
Being frequently exposed to foreign nucleic acids, bacteria and archaea have developed an ingenious adaptive defense system, called CRISPR-Cas. The system is composed of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) array, together with CRISPR (cas)-associated genes. This system consists of a complex machinery that integrates fragments of foreign nucleic acids from viruses
-
Natural Products from Nocardia and Their Role in Pathogenicity. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-17 Alicia Engelbrecht,Hamada Saad,Harald Gross,Leonard Kaysser
Nocardia spp. are filamentous Actinobacteria of the order Corynebacteriales and mostly known for their ability to cause localized and systemic infections in humans. However, the onset and progression of nocardiosis is only poorly understood, in particular the mechanisms of strain-specific presentations. Recent genome sequencing has revealed an extraordinary capacity for the production of specialized
-
5-Deoxyadenosine Metabolism: More than "Waste Disposal". Microb. Physiol. (IF 1.2) Pub Date : 2021-06-14 Johanna Rapp,Karl Forchhammer
5-Deoxyadenosine (5dAdo) is a by-product of many radical SAM enzyme reactions in all domains of life, and an inhibitor of the radical SAM enzymes themselves. Hence, pathways to recycle or dispose of this toxic by-product must exist but remain largely unexplored. In this review, we discuss the current knowledge about canonical and atypical 5dAdo salvage pathways that have been characterized in the last
-
The Role of hlb-Converting Bacteriophages in Staphylococcus aureus Host Adaption. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-14 Carina Rohmer,Christiane Wolz
As an opportunistic pathogen of humans and animals, Staphylococcus aureus asymptomatically colonizes the nasal cavity but is also a leading cause of life-threatening acute and chronic infections. The evolution of S. aureus resulting from short- and long-term adaptation to diverse hosts is tightly associated with mobile genetic elements. S. aureus strains can carry up to four temperate phages, many
-
The Epipeptide Biosynthesis Locus epeXEPAB Is Widely Distributed in Firmicutes and Triggers Intrinsic Cell Envelope Stress. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-11 Philipp F Popp,Lena Friebel,Alhosna Benjdia,Alain Guillot,Olivier Berteau,Thorsten Mascher
The epeXEPAB (formerly yydFGHIJ) locus of Bacillus subtilis encodes a minimalistic biosynthetic pathway for a linear antimicrobial epipeptide, EpeX, which is ribosomally produced and post-translationally processed by the action of the radical-SAM epimerase, EpeE, and a membrane-anchored signal 2 peptide peptidase, EpeP. The ABC transporter EpeAB provides intrinsic immunity against self-produced EpeX
-
Amyloid Proteins in Plant-Associated Microbial Communities. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-09 Daniel Gómez-Pérez,Vasvi Chaudhry,Ariane Kemen,Eric Kemen
Amyloids have proven to be a widespread phenomenon rather than an exception. Many proteins presenting the hallmarks of this characteristic beta sheet-rich folding have been described to date. Particularly common are functional amyloids that play an important role in the promotion of survival and pathogenicity in prokaryotes. Here, we describe important developments in amyloid protein research that
-
Peptidoglycan Salvage Enables the Periodontal Pathogen Tannerella forsythia to Survive within the Oral Microbial Community. Microb. Physiol. (IF 1.2) Pub Date : 2021-06-09 Isabel Hottmann,Marina Borisova,Christina Schäffer,Christoph Mayer
Tannerella forsythia is an anaerobic, fusiform Gram-negative oral pathogen strongly associated with periodontitis, a multibacterial inflammatory disease that leads to the destruction of the teeth-supporting tissue, ultimately causing tooth loss. To survive in the oral habitat, T. forsythia depends on cohabiting bacteria for the provision of nutrients. For axenic growth under laboratory conditions,
-
Diversity in Starvation Survival Strategies and Outcomes among Heterotrophic Proteobacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-05-31 Megan Bergkessel,Laurent Delavaine
Heterotrophic Proteobacteria are versatile opportunists that have been extensively studied as model organisms in the laboratory, as both pathogens and beneficial symbionts of plants and animals, and as ubiquitous organisms found free-living in many environments. Succeeding in these niches requires an ability to persist for potentially long periods of time in growth-arrested states when essential nutrients
-
Poly- and Monoamine Metabolism in Streptomyces coelicolor: The New Role of Glutamine Synthetase-Like Enzymes in the Survival under Environmental Stress. Microb. Physiol. (IF 1.2) Pub Date : 2021-05-27 Sergii Krysenko,Arne Matthews,Tobias Busche,Agnieszka Bera,Wolfgang Wohlleben
Soil bacteria from the genus Streptomyces, phylum Actinobacteria, feature a complex metabolism and diverse adaptations to environmental stress. These characteristics are consequences of variable nutrition availability in the soil and allow survival under changing nitrogen conditions. Streptomyces coelicolor is a model organism for Actinobacteria and is able to use nitrogen from a variety of sources
-
The Multiple Roles of Polyphosphate in Ralstonia eutropha and Other Bacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-05-20 Hanna Rosigkeit,Lea Kneißle,Stanislav Obruča,Dieter Jendrossek
An astonishing variety of functions has been attributed to polyphosphate (polyP) in prokaryotes. Besides being a reservoir of phosphorus, functions in exopolysaccharide formation, motility, virulence and in surviving various forms of stresses such as exposure to heat, extreme pH, oxidative agents, high osmolarity, heavy metals and others have been ascribed to polyP. In this contribution, we will provide
-
Polyhydroxybutyrate: A Useful Product of Chlorotic Cyanobacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-05-12 Moritz Koch,Karl Forchhammer
Polyhydroxybutyrate (PHB) is a carbon polymer with diverse functions, varying greatly on the organism producing it. This microreview describes the current knowledge about PHB metabolism, structure, and different physiological roles with a special focus on cyanobacteria. Despite the physiological function of PHB in the cyanobacterial phylum still being unknown, these organisms provide the unique opportunity
-
Recovery of Unicellular Cyanobacteria from Nitrogen Chlorosis: A Model for Resuscitation of Dormant Bacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-04-20 Niels Neumann,Sofia Doello,Karl Forchhammer
Nitrogen starvation induces developmental transitions in cyanobacteria. Whereas complex multicellular cyanobacteria of the order Nostocales can differentiate specialized cells that perform nitrogen fixation in the presence of oxygenic photosynthesis, non-diazotrophic unicellular strains, such as Synechococcus elongatus or Synechocystis PCC 6803, undergo a transition into a dormant non-growing state
-
Recovery of Unicellular Cyanobacteria from Nitrogen Chlorosis: A Model for Resuscitation of Dormant Bacteria. Microb. Physiol. (IF 1.2) Pub Date : 2021-04-20 Niels Neumann,Sofia Doello,Karl Forchhammer
Nitrogen starvation induces developmental transitions in cyanobacteria. Whereas complex multicellular cyanobacteria of the order Nostocales can differentiate specialized cells that perform nitrogen fixation in the presence of oxygenic photosynthesis, non-diazotrophic unicellular strains, such as Synechococcus elongatus or Synechocystis PCC 6803, undergo a transition into a dormant non-growing state
-
The Genetics of Prey Susceptibility to Myxobacterial Predation: A Review, Including an Investigation into Pseudomonas aeruginosa Mutations Affecting Predation by Myxococcus xanthus. Microb. Physiol. (IF 1.2) Pub Date : 2021-04-01 Natashia Sydney,Martin T Swain,Jeffery M T So,Egbert Hoiczyk,Nicholas P Tucker,David E Whitworth
Bacterial predation is a ubiquitous and fundamental biological process, which influences the community composition of microbial ecosystems. Among the best characterised bacterial predators are the myxobacteria, which include the model organism Myxococcus xanthus. Predation by M. xanthus involves the secretion of antibiotic metabolites and hydrolytic enzymes, which results in the lysis of prey organisms
-
Chitin, Chitin Oligosaccharide, and Chitin Disaccharide Metabolism of Escherichia coli Revisited: Reassignment of the Roles of ChiA, ChbR, ChbF, and ChbG. Microb. Physiol. (IF 1.2) Pub Date : 2021-04-01 Axel Walter,Simon Friz,Christoph Mayer
Escherichia coli is unable to grow on polymeric and oligomeric chitin, but grows on chitin disaccharide (GlcNAc-GlcNAc; N,N'-diacetylchitobiose) and chitin trisaccharide (GlcNAc-GlcNAc-GlcNAc; N,N',N''-triacetylchitotriose) via expression of the chb operon (chbBCARFG). The phosphotransferase system (PTS) transporter ChbBCA facilitates transport of both saccharides across the inner membrane and their
-
Chitin, Chitin Oligosaccharide, and Chitin Disaccharide Metabolism of Escherichia coli Revisited: Reassignment of the Roles of ChiA, ChbR, ChbF, and ChbG. Microb. Physiol. (IF 1.2) Pub Date : 2021-04-01 Axel Walter,Simon Friz,Christoph Mayer
Escherichia coli is unable to grow on polymeric and oligomeric chitin, but grows on chitin disaccharide (GlcNAc-GlcNAc; N,N'-diacetylchitobiose) and chitin trisaccharide (GlcNAc-GlcNAc-GlcNAc; N,N',N''-triacetylchitotriose) via expression of the chb operon (chbBCARFG). The phosphotransferase system (PTS) transporter ChbBCA facilitates transport of both saccharides across the inner membrane and their
-
Analysis of Kytococcus sedentarius Strain Isolated from a Dehumidifier Operating in a University Lecture Theatre: Systems for Aerobic Respiration, Resisting Osmotic Stress, and Sensing Nitric Oxide. Microb. Physiol. (IF 1.2) Pub Date : 2021-03-17 Meshari Ahmed Alhadlaq,Jeffrey Green,Bassam K Kudhair
A strain of Kytococcus sedentarius was isolated from a dehumidifier operating in a university lecture theatre. Genome analysis and phenotypic characterisation showed that this strain, K. sedentarius MBB13, was a moderately halotolerant aerobe with a branched aerobic electron transport chain and genes that could contribute to erythromycin resistance. The major compatible solute was glycine betaine,
-
Analysis of Kytococcus sedentarius Strain Isolated from a Dehumidifier Operating in a University Lecture Theatre: Systems for Aerobic Respiration, Resisting Osmotic Stress, and Sensing Nitric Oxide. Microb. Physiol. (IF 1.2) Pub Date : 2021-03-17 Meshari Ahmed Alhadlaq,Jeffrey Green,Bassam K Kudhair
A strain of Kytococcus sedentarius was isolated from a dehumidifier operating in a university lecture theatre. Genome analysis and phenotypic characterisation showed that this strain, K. sedentarius MBB13, was a moderately halotolerant aerobe with a branched aerobic electron transport chain and genes that could contribute to erythromycin resistance. The major compatible solute was glycine betaine,
-
Proteogenomic Insights into the Physiology of Marine, Sulfate-Reducing, Filamentous Desulfonema limicola and Desulfonema magnum. Microb. Physiol. (IF 1.2) Pub Date : 2021-02-19 Vanessa Schnaars,Lars Wöhlbrand,Sabine Scheve,Christina Hinrichs,Richard Reinhardt,Ralf Rabus
The genus Desulfonema belongs to the deltaproteobacterial family Desulfobacteraceae and comprises marine, sulfate-reducing bacteria that form filaments and move by gliding. This study reports on the complete, manually annotated genomes of Dn. limicola 5ac10T (6.91 Mbp; 6,207 CDS) and Dn. magnum 4be13T (8.03 Mbp; 9,970 CDS), integrated with substrate-specific proteome profiles (8 vs. 11). The richness
-
Complete Genomes of the Anaerobic Degradation Specialists Aromatoleum petrolei ToN1T and Aromatoleum bremense PbN1T. Microb. Physiol. (IF 1.2) Pub Date : 2021-01-21 Arne Weiten,Kristin Kalvelage,Patrick Becker,Richard Reinhardt,Thomas Hurek,Barbara Reinhold-Hurek,Ralf Rabus
The betaproteobacterial genus Aromatoleum comprises facultative denitrifiers specialized in the anaerobic degradation of recalcitrant organic compounds (aromatic and terpenoid). This study reports on the complete and manually annotated genomes of Ar. petrolei ToN1T (5.41 Mbp) and Ar. bremense PbN1T (4.38 Mbp), which cover the phylogenetic breadth of the genus Aromatoleum together with previously genome
-
Complete Genomes of the Anaerobic Degradation Specialists Aromatoleum petrolei ToN1T and Aromatoleum bremense PbN1T. Microb. Physiol. (IF 1.2) Pub Date : 2021-01-21 Arne Weiten,Kristin Kalvelage,Patrick Becker,Richard Reinhardt,Thomas Hurek,Barbara Reinhold-Hurek,Ralf Rabus
The betaproteobacterial genus Aromatoleum comprises facultative denitrifiers specialized in the anaerobic degradation of recalcitrant organic compounds (aromatic and terpenoid). This study reports on the complete and manually annotated genomes of Ar. petrolei ToN1T (5.41 Mbp) and Ar. bremense PbN1T (4.38 Mbp), which cover the phylogenetic breadth of the genus Aromatoleum together with previously genome
-
Ultraviolet-B Radiation Stress-Induced Toxicity and Alterations in Proteome of Deinococcus radiodurans. Microb. Physiol. (IF 1.2) Pub Date : 2020-12-18 Jay Kumar,Paushali Ghosh,Ashok Kumar
Deinococcus radiodurans is a polyextremophilic bacterium capable to survive and grow at high doses of ionizing radiation. Besides resistance to ionizing radiation, the bacterium is also resistant to toxic chemicals and desiccation. This study deals with the effects of non-ionizing radiation (ultraviolet-B) on survival, alterations in proteomic profile, and gene expression in D. radiodurans. Exposure
-
Ultraviolet-B Radiation Stress-Induced Toxicity and Alterations in Proteome of Deinococcus radiodurans. Microb. Physiol. (IF 1.2) Pub Date : 2020-12-18 Jay Kumar,Paushali Ghosh,Ashok Kumar
Deinococcus radiodurans is a polyextremophilic bacterium capable to survive and grow at high doses of ionizing radiation. Besides resistance to ionizing radiation, the bacterium is also resistant to toxic chemicals and desiccation. This study deals with the effects of non-ionizing radiation (ultraviolet-B) on survival, alterations in proteomic profile, and gene expression in D. radiodurans. Exposure
-
-
-
Efficacy of Xyloglucan against Escherichia coli Extraintestinal Urinary Tract Infection: An in vivo Study. Microb. Physiol. (IF 1.2) Pub Date : 2020-10-16 Emanuela Esposito,Michela Campolo,Giovanna Casili,Marika Lanza,Domenico Franco,Enza Fazio,Alessia Filippone,Irene Paterniti,Salvatore Cuzzocrea
Natural approaches to conventional pharmaceutical treatments for urinary tract infections (UTIs) have focused attention toward reducing the colonization of intestinal Escheri-chia coli reservoirs, the cause of ascending and hematogenous UTIs. In this study, we evaluated the protective effect of xyloglucan and xyloglucan plus gelose on intestinal and urinary epithelia in an in vivo E. coli infection
-
Efficacy of Xyloglucan against Escherichia coli Extraintestinal Urinary Tract Infection: An in vivo Study. Microb. Physiol. (IF 1.2) Pub Date : 2020-10-16 Emanuela Esposito,Michela Campolo,Giovanna Casili,Marika Lanza,Domenico Franco,Enza Fazio,Alessia Filippone,Irene Paterniti,Salvatore Cuzzocrea
Natural approaches to conventional pharmaceutical treatments for urinary tract infections (UTIs) have focused attention toward reducing the colonization of intestinal Escheri-chia coli reservoirs, the cause of ascending and hematogenous UTIs. In this study, we evaluated the protective effect of xyloglucan and xyloglucan plus gelose on intestinal and urinary epithelia in an in vivo E. coli infection
-
Protein-Protein Interactions in the Cytoplasmic Membrane of Escherichia coli: Influence of the Overexpression of Diverse Transporter-Encoding Genes on the Activities of PTS Sugar Uptake Systems. Microb. Physiol. (IF 1.2) Pub Date : 2020-09-30 Mohammad Aboulwafa,Zhongge Zhang,Milton H Saier
The prokaryotic phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) concomitantly transports and phosphorylates its substrate sugars. In a recent publication, we provided evidence that protein-protein interactions of the fructose-specific integral membrane transporter (FruAB) with other PTS sugar group translocators regulate the activities of the latter systems in vivo and sometimes in
-
The Causal Relationship between Eating Animals and Viral Epidemics. Microb. Physiol. (IF 1.2) Pub Date : 2020-09-21 Bhaskara L Reddy,Milton H Jr Saier
For decades it has been known that infectious agents including pathogenic protozoans, bacteria, and viruses, adapted to a particular animal host, can mutate to gain the ability to infect another host, and the mechanisms involved have been studied in great detail. Although an infectious agent in one animal can alter its host range with relative ease, no example of a plant virus changing its host organism
-
Deletion of PIN4 Suppresses the Protein Transport Defects Caused by sec12-4 Mutation in Saccharomyces cerevisiae. Microb. Physiol. (IF 1.2) Pub Date : 2020-09-15 Akiko Murakami-Sekimata,Masayuki Sekimata,Natsumi Sato,Yuto Hayasaka,Akihiko Nakano
Newly synthesized secretory proteins are released into the lumen of the endoplasmic reticulum (ER). The secretory proteins are surrounded by coat protein complex II (COPII) vesicles, and transported from the ER and reach their destinations through the Golgi apparatus. Sec12p is a guanine nucleotide exchange factor for Sar1p, which initiates COPII vesicle budding from the ER. The activation of Sar1p
-
Global Response of Phaeobacter inhibens DSM 17395 to Deletion of Its 262-kb Chromid Encoding Antibiotic Synthesis. Microb. Physiol. (IF 1.2) Pub Date : 2020-07-24 Daniel Wünsch,Annemieke Strijkstra,Lars Wöhlbrand,Heike M Freese,Sabine Scheve,Christina Hinrichs,Kathleen Trautwein,Michael Maczka,Jörn Petersen,Stefan Schulz,Jörg Overmann,Ralf Rabus
The marine alphaproteobacterium Phaeobacter inhibens DSM 17395, a member of the Roseobacter group, was recently shown to markedly enhance growth upon deletion of its 262-kb chromid encoding biosynthesis of tropodithietic acid (TDA). To scrutinize the metabolic/regulatory adaptations that underlie enhanced growth of the Δ262 mutant, its transcriptome and proteome compared to the wild type were investigated
-
-
Immunogenicity Evaluation of Chimeric Subunit Vaccine Comprising Adhesion Coli Surface Antigens from Enterotoxigenic Escherichia coli. Microb. Physiol. (IF 1.2) Pub Date : 2020-07-09 Dorna Khoobbakht,Shohreh Zare Karizi,Mohammad Javad Motamedi,Rouhollah Kazemi,Pooneh Roghanian,Jafar Amani
Enterotoxigenic Escherichia coli (ETEC) is the most common agent of diarrhea morbidity in developing countries. ETEC adheres to host intestinal epithelial cells via various colonization factors. The CooD and CotD proteins play a significant role in bacteria binding to the intestinal epithelial cells as adhesin tip subunits of CS1 and CS2 pili. The purpose here was to design a new construction containing
-
Study of the Enzymatic Capacity of Kluyveromyces marxianus for the Synthesis of Esters. Microb. Physiol. (IF 1.2) Pub Date : 2020-04-23 Francisco Javier Reyes-Sánchez,Jesús Bernardo Páez-Lerma,Juan Antonio Rojas-Contreras,Javier López-Miranda,Nicolás Óscar Soto-Cruz,Manuel Reinhart-Kirchmayr
Recently, biotechnological opportunities have been found in non-Saccharomyces yeasts because they possess metabolic characteristics that lead to the production of compounds of interest. It has been observed that Kluyveromyces marxianus has a great potential in the production of esters, which are aromatic compounds of industrial importance. The genetic bases that govern the synthesis of esters include
-
Vaccination of Mice with Listeria ivanovii Expressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+ and CD8+ T Cell-Mediated Immunity. Microb. Physiol. (IF 1.2) Pub Date : 2020-04-14 Tian Tang,Chuan Wang,Qikang Pu,Jinmei Peng,Sijing Liu,Chenyan Ren,Mingjuan Jiang,Zhijun Tian
Porcine reproductive and respiratory syndrome (PRRS), a serious disease of swine caused by the PRRS virus (PRRSV), had a severe economic impact worldwide. As commonly used PRRS vaccines, the attenuated or inactivated vaccines, provide unsatisfactory immune protection, a new PRRS vaccine is urgently needed. In this study, a part of the PRRSV ORF6 gene (from 253 to 519 bp) encoding the hydrophilic domain
-
Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus. Microb. Physiol. (IF 1.2) Pub Date : 2020-04-07 Luciana Villafañe,Marina Andrea Forrellad,María Gabriela López,Sergio Garbaccio,Carlos Garro,Rosana Valeria Rocha,María Emilia Eirin,Mahavir Singh,Oscar A Taboga,Fabiana Bigi
Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative of M. bovis (PPDb). Another ancillary bTB test detects IFN-γ produced in whole blood upon stimulation with PPDb, protein/peptide cocktails