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Ribosomal Protein Cluster Organization in Asgard Archaea Archaea (IF 2.4) Pub Date : 2023-9-29 Madhan R. Tirumalai, Raghavan V. Sivaraman, Layla A. Kutty, Eric L. Song, George E. Fox
It has been proposed that the superphylum of Asgard Archaea may represent a historical link between the Archaea and Eukarya. Following the discovery of the Archaea, it was soon appreciated that archaeal ribosomes were more similar to those of Eukarya rather than Bacteria. Coupled with other eukaryotic-like features, it has been suggested that the Asgard Archaea may be directly linked to eukaryotes
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VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting Halorubrum Archaea (IF 2.4) Pub Date : 2021-12-14 Shaoxing Chen, Yongpei Dai, Jingwen Liu, Shimin Zhang, Feilong Chen, Fanjie Jin, Peiyao Ruan, Lu Li, Xiangdong Chen
A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon Halorubrum sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is nm, and uncontracted tails are nm. The latent period is about
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The Ability of Hop Extracts to Reduce the Methane Production of Methanobrevibacter ruminantium Archaea (IF 2.4) Pub Date : 2021-11-05 J. A. Blaxland, A. J. Watkins, L. W. J. Baillie
Background. Methane emissions from agriculture are responsible for over 40% of the world’s greenhouse gas emissions. In the past, antibiotics were used to control methane production by animals, but concerns over the emergence and spread of antibiotic-resistant bacteria to humans have prompted a search for alternative approaches. Hops are the flowers of the hop plant Humulus lupulus. They have been
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Biological Processes for Pollution Control: Current Research and Emerging Technologies 2020. Archaea (IF 2.4) Pub Date : 2021-11-05 Jin Li,Yu Tao,Guangbin Li,Cuijie Feng,Rong Chen,Ming Hua
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Structural and Kinetic Characterization of Hyperthermophilic NADH-Dependent Persulfide Reductase from Archaeoglobus fulgidus Archaea (IF 2.4) Pub Date : 2021-03-10 Sherwin Shabdar, Bukuru Anaclet, Ana Garcia Castineiras, Neyissa Desir, Nicholas Choe, Edward J. Crane, Matthew H. Sazinsky
NADH-dependent persulfide reductase (Npsr) has been proposed to facilitate dissimilatory sulfur respiration by reducing persulfide or sulfane sulfur-containing substrates to H2S. The presence of this gene in the sulfate and thiosulfate-reducing Archaeoglobus fulgidus DSM 4304 and other hyperthermophilic Archaeoglobales appears anomalous, as A. fulgidus is unable to respire S0 and grow in the presence
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Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode Archaea (IF 2.4) Pub Date : 2021-03-02 Hajime Kobayashi, Ryohei Toyoda, Hiroyuki Miyamoto, Yasuhito Nakasugi, Yuki Momoi, Kohei Nakamura, Qian Fu, Haruo Maeda, Takashi Goda, Kozo Sato
Electromethanogenesis refers to the bioelectrochemical synthesis of methane from CO2 by biocathodes. In an electromethanogenic system using thermophilic microorganisms, metagenomic analysis along with quantitative real-time polymerase chain reaction and fluorescence in situ hybridization revealed that the biocathode microbiota was dominated by the methanogen Methanothermobacter sp. strain EMTCatA1
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Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio Archaea (IF 2.4) Pub Date : 2021-02-10 Viola Corbellini, Cuijie Feng, Micol Bellucci, Arianna Catenacci, Tatiana Stella, Anna Espinoza-Tofalos, Francesca Malpei
The effect of the amount of hydrogen supplied for the in situ biological biogas upgrading was investigated by monitoring the process and evolution of the microbial community. Two parallel reactors, operated at 37°C for 211 days, were continuously fed with sewage sludge at a constant organic loading rate of 1.5 gCOD∙(L∙d)-1 and hydrogen (H2). The molar ratio of H2/CO2 was progressively increased from
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Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source. Archaea (IF 2.4) Pub Date : 2021-01-26 Fatma Karray,Manel Ben Abdallah,Nidhal Baccar,Hatem Zaghden,Sami Sayadi
Microbial production of bioplastics, derived from poly(3-hydroxybutyrate) (PHB), have provided a promising alternative towards plastic pollution. Compared to other extremophiles, halophilic archaea are considered as cell factories for PHB production by using renewable, inexpensive carbon sources, thus decreasing the fermentation cost. This study is aimed at screening 33 halophilic archaea isolated
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Flocculation Efficiency and Mechanism of Carbamazepine by Microbial Flocculant Extracted from Klebsiella pneumoniae J1 Archaea (IF 2.4) Pub Date : 2020-11-18 Jie Xing, Nanzhe Song, Xiangwei Chen, Ang Li, Hongwei Ni
The microbial flocculant (MFX) extracted from Klebsiella pneumoniae J1 was used to remove carbamazepine in prepared wastewater and domestic sewage. The influence factors and flocculation mechanism were studied. The optimal carbamazepine removal conditions for MFX were pH of 7-8, 7 mL of flocculant, 0.1 mL of coagulant, and 35°C, and the removal rate reached 81.75%. MFX was efficient in the removal
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Thermophilic Solid-State Anaerobic Digestion of Corn Straw, Cattle Manure, and Vegetable Waste: Effect of Temperature, Total Solid Content, and C/N Ratio Archaea (IF 2.4) Pub Date : 2020-11-11 Lianghu Su, Xu Sun, Chenwei Liu, Rongting Ji, Guangyin Zhen, Mei Chen, Longjiang Zhang
Thermophilic solid-state anaerobic digestion (SS-AD) of agricultural wastes, i.e., corn straw, cattle manure, and vegetable waste, was carried out in this study. The effects of temperature (40-60°C), initial solid content (ISC, 17.5-32.5%), and C/N ratio (15-32 : 1) on biogas production were evaluated using a Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). The
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Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation Archaea (IF 2.4) Pub Date : 2020-11-09 Abirami Sasi, Nagarajan Duraipandiyan, Kannan Marikani, Sugapriya Dhanasekaran, Noura Al-Dayan, Divya Venugopal
Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis. A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with
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A Current Sensing Biosensor for BOD Rapid Measurement Archaea (IF 2.4) Pub Date : 2020-10-26 Yiman Liu, Jie Li, Nianxin Wan, Tianyu Fu, Lili Wang, Cong Li, Zhonghui Qie, Ao Zhu
In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, Bacillus subtilis was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms
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Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions In Vitro Archaea (IF 2.4) Pub Date : 2020-10-22 Mubarik Mahmood, Ratchaneewan Khiaosa-ard, Qendrim Zebeli, Renée M. Petri
Rumen archaea play an important role in scavenging ruminal hydrogen (H2) and thus facilitate rumen fermentation. They require optimum temperature and osmolality for their growth and metabolism; however, a number of external factors may put archaea under heat and osmotic stress. Betaine is an osmolyte, molecular chaperone, and antioxidant; therefore, it bears potential to combat against these stressors
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Effects of Sludge Retention Time on the Performance of Anaerobic Ceramic Membrane Bioreactor Treating High-Strength Phenol Wastewater. Archaea (IF 2.4) Pub Date : 2020-08-01 Chunhua He,Chuanhe Yang,Shoujun Yuan,Zhenhu Hu,Wei Wang
Anaerobic ceramic membrane bioreactor (AnCMBR) is an attractive alternative for the treatment of high-strength phenol wastewater, but the effects of sludge retention time (SRT) on the performance and membrane fouling are still unclear. The results indicated that the AnCMBR was successfully employed to treat high-strength wastewater containing 5 g phenol L-1. The removal efficiencies of phenol and chemical
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Simulation of a Novel Tubular Microalgae Photobioreactor with Aerated Tangent Inner Tubes: Improvements in Mixing Performance and Flashing-Light Effects. Archaea (IF 2.4) Pub Date : 2020-07-10 Xuyang Cui,Junhong Yang,Yuanzheng Feng,Wenwen Zhang
At present, large-scale and high-efficiency microalgal cultivation is the key to realizing the technology for carbon capture and storage (CCS) and bioresource recovery. Meanwhile, tubular photobioreactors (PBRs) have great potential for microalgal cultivation due to their high productivity. To improve the mixing performance and flashing-light effect, a novel tube PBR with the inner tube tangential
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Response and Adaptation of Microbial Community in a CANON Reactor Exposed to an Extreme Alkaline Shock. Archaea (IF 2.4) Pub Date : 2020-07-01 Ruili Yang,Wenlong Mao,Xiaojun Wang,Zhaoji Zhang,Junbin Wu,Shaohua Chen
Responses of a microbial community in the completely autotrophic nitrogen removal over nitrite (CANON) process, which was shocked by a pH of 11.0 for 12 h, were investigated. During the recovery phase, the performance, anaerobic ammonia oxidation (anammox) activity, microbial community, and correlation of bacteria as well as the influencing factors were evaluated synchronously. The performance of the
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Biosorption Mechanism of Aqueous Pb2+, Cd2+, and Ni2+ Ions on Extracellular Polymeric Substances (EPS). Archaea (IF 2.4) Pub Date : 2020-06-24 Di Cui,Chong Tan,Hongna Deng,Xunxue Gu,Shanshan Pi,Ting Chen,Lu Zhou,Ang Li
Heavy metal pollution has been a focus with increasing attention, especially Pb2+, Cd2+, and Ni2+ in an aqueous environment. The adsorption capacity and mechanism of extracellular polymeric substances (EPS) from Agrobacterium tumefaciens F2 for three heavy metals were investigated in this study. The adsorption efficiency of 94.67%, 94.41%, and 77.95% were achieved for Pb2+, Cd2+, and Ni2+ adsorption
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Integration of Marine Macroalgae (Chaetomorpha maxima) with a Moving Bed Bioreactor for Nutrient Removal from Maricultural Wastewater. Archaea (IF 2.4) Pub Date : 2020-06-23 Xian Li,Yale Deng,Xueying Li,Xiaona Ma,Jinxia Wang,Jun Li
Rather than direct nutrient removal from wastewaters, an alternative approach aimed at nutrient recovery from aquacultural wastewaters could enable sustainable management for aquaculture production. This study demonstrated the feasibility of cultivating marine macroalgae (Chaetomorpha maxima) with a moving bed bioreactor (MBBR-MA), to remove nitrogen and phosphorus in aquaculture wastewater as well
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Characteristics of Biohydrogen Production and Performance of Hydrogen-Producing Acetogen by Increasing Normal Molasses Wastewater Proportion in Anaerobic Baffled Reactor. Archaea (IF 2.4) Pub Date : 2020-06-05 Xuejia Gu,Yufeng Wang,Huaibo Li,Ji Li,Shuo Wang
The biohydrogen production efficiency and performance of hydrogen-producing acetogen in a four-compartment anaerobic baffled reactor (ABR) were studied by gradually increasing the influent normal molasses wastewater (NMWW) proportion. When the influent NMWW proportion increased to 55%, ABR could develop microbial community with methanogenic function in 63 days and reach a stable operation. When the
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Functional Group Distribution of the Carrier Surface Influences Adhesion of Methanothermobacter thermautotrophicus. Archaea (IF 2.4) Pub Date : 2020-01-23 Masaki Umetsu,Takaaki Sunouchi,Yasuhiro Fukuda,Hideyuki Takahashi,Chika Tada
Various support carriers are used for high-density retention of methanogenic archaea in anaerobic wastewater treatment systems. Although the physicochemical properties of carrier materials and microorganisms influence the adhesion of methanogenic archaea, details about the underlying mechanism remain poorly characterized. We applied seven types of chemical surface modifications to carbon felts to clarify
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Community Structure and Abundance of Archaea in a Zostera marina Meadow: A Comparison between Seagrass-Colonized and Bare Sediment Sites. Archaea (IF 2.4) Pub Date : 2019-11-18 Pengfei Zheng,Chuantao Wang,Xiaoli Zhang,Jun Gong
Seagrass colonization alters sediment physicochemical properties by depositing seagrass fibers and releasing organic carbon and oxygen from the roots. How this seagrass colonization-induced spatial heterogeneity affects archaeal community structure and abundance remains unclear. In this study, we investigated archaeal abundance, diversity, and composition in both vegetated and adjacent bare surface
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Archaea in Wastewater Treatment: Current Research and Emerging Technology. Archaea (IF 2.4) Pub Date : 2018-12-26 Jin Li,Rutao Liu,Yu Tao,Guangbin Li
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Acetotrophic Activity Facilitates Methanogenesis from LCFA at Low Temperatures: Screening from Mesophilic Inocula. Archaea (IF 2.4) Pub Date : 2019-05-02 Suniti Singh,Johanna M Rinta-Kanto,Riitta Kettunen,Piet Lens,Gavin Collins,Marika Kokko,Jukka Rintala
The inoculum source plays a crucial role in the anaerobic treatment of wastewaters. Lipids are present in various wastewaters and have a high methanogenic potential, but their hydrolysis results in the production of long chain fatty acids (LCFAs) that are inhibitory to anaerobic microorganisms. Screening of inoculum for the anaerobic treatment of LCFA-containing wastewaters has been performed at mesophilic
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The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media. Archaea (IF 2.4) Pub Date : 2019-04-24 Anna M Vetter,Julia Helmecke,Dietmar Schomburg,Meina Neumann-Schaal
Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of
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Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean. Archaea (IF 2.4) Pub Date : 2019-03-03 Franziska Wemheuer,Avril Jean Elisabeth von Hoyningen-Huene,Marion Pohlner,Julius Degenhardt,Bert Engelen,Rolf Daniel,Bernd Wemheuer
Information on environmental conditions shaping archaeal communities thriving at the seafloor of the central Pacific Ocean is limited. The present study was conducted to investigate the diversity, composition, and function of both entire and potentially active archaeal communities within Pacific deep-sea sediments. For this purpose, sediment samples were taken along the 180° meridian of the central
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Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate. Archaea (IF 2.4) Pub Date : 2019-02-11 Giada Lo Gullo,Rosanna Mattossovich,Giuseppe Perugino,Anna La Teana,Paola Londei,Dario Benelli
A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for translation of pretranscribed mRNAs, and now adapted to perform coupled transcription and translation. The essential element in this expression system is a
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Long-Term Effects of Periodical Fires on Archaeal Communities from Brazilian Cerrado Soils. Archaea (IF 2.4) Pub Date : 2019-01-27 Aline Belmok,Thiago Rodrigues-Oliveira,Fabyano A C Lopes,Heloisa S Miranda,Ricardo H Krüger,Cynthia M Kyaw
The Cerrado biome corresponds to an extensive area of Brazil and is considered a biodiversity hotspot. Frequent fires are a natural feature in this biome and have influences on vegetation structure and composition. However, continuous anthropogenic actions are promoting changes in fire frequency and seasonality. Despite the high biodiversity of the Cerrado, little is known about its microbiome, with
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Crystal Structures of the Putative Isocitrate Dehydrogenase from Sulfolobus tokodaii Strain 7 in the Apo and NADP+-Bound Forms. Archaea (IF 2.4) Pub Date : 2018-12-19 Hisanori Kondo,Midori Murakami
Isocitrate dehydrogenase is a catabolic enzyme that acts during the third step of the tricarboxylic acid cycle. The hypothetical protein ST2166 from the archaeon Sulfolobus tokodaii was isolated and crystallized. It shares high primary structure homology with prokaryotic NADP+-dependent IDHs, suggesting that these enzymes share a common enzymatic mechanism. The crystal structure of ST2166 was determined
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The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink. Archaea (IF 2.4) Pub Date : 2018-11-08 Yanfen Zhang,Anzhou Ma,Wenzong Liu,Zhihui Bai,Xuliang Zhuang,Guoqiang Zhuang
Recently, a new oxygenic pathway has been proposed based on the disproportionation of NO with putative NO dismutase (Nod). In addition to a new process in nitrogen cycling, this process provides ecological advantages for the degradation of substrates in anaerobic conditions, which is of great significance for wastewater treatment. However, the Nod distribution in aquatic environments is rarely investigated
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Variation of Bacterial and Archaeal Community Structures in a Full-Scale Constructed Wetlands for Wastewater Treatment. Archaea (IF 2.4) Pub Date : 2018-10-16 Xiu-Lu Lang,Xiang Chen,Ai-Ling Xu,Zhi-Wen Song,Xin Wang,He-Bing Wang
Microorganisms play important roles in the reduction of organic and inorganic pollutants in constructed wetlands used for the treatment of wastewater. However, the diversity and structure of microbial community in constructed wetland system remain poorly known. In this study, the Illumina MiSeq Sequencing of 16S rDNA was used to analyze the bacterial and archaeal microbial community structures of soil
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Ammonia-Oxidizing Archaea (AOA) Play with Ammonia-Oxidizing Bacteria (AOB) in Nitrogen Removal from Wastewater. Archaea (IF 2.4) Pub Date : 2018-09-13 Zhixuan Yin,Xuejun Bi,Chenlu Xu
An increase in the number of publications in recent years indicates that besides ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) may play an important role in nitrogen removal from wastewater, gaining wide attention in the wastewater engineering field. This paper reviews the current knowledge on AOA and AOB involved in wastewater treatment systems and summarises the environmental
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Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis. Archaea (IF 2.4) Pub Date : 2018-09-06 Huihui Wang,Shaoping Kuang,Qiaolin Lang,Wenjuan Yu
The oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil sludge significantly changed physical and chemical properties of the receiving soil and increased the
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Treatment of Landfill Leachate Using Activated Sludge Technology: A Review. Archaea (IF 2.4) Pub Date : 2018-09-04 Kai Wang,Lusheng Li,Fengxun Tan,Daoji Wu
Landfill leachate contains a large amount of organic matter and ammoniacal nitrogen. As such, it has become a complex and difficult issue within the water treatment industry. The activated sludge process has been found to be a good solution with low processing costs and is now therefore the core process for leachate treatment, especially for nitrogen removal. This paper describes the characteristics
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Diversity and Niche of Archaea in Bioremediation. Archaea (IF 2.4) Pub Date : 2018-09-03 Mark James Krzmarzick,David Kyle Taylor,Xiang Fu,Aubrey Lynn McCutchan
Bioremediation is the use of microorganisms for the degradation or removal of contaminants. Most bioremediation research has focused on processes performed by the domain Bacteria; however, Archaea are known to play important roles in many situations. In extreme conditions, such as halophilic or acidophilic environments, Archaea are well suited for bioremediation. In other conditions, Archaea collaboratively
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Soil Microbial Community Structure and Diversity around the Aging Oil Sludge in Yellow River Delta as Determined by High-Throughput Sequencing. Archaea (IF 2.4) Pub Date : 2018-08-30 Shaoping Kuang,Yaqing Su,Huihui Wang,Wenjuan Yu,Qiaolin Lang,Ravichandra Matangi
Microorganisms are sensitive indicators of edaphic environmental variation. The Illumina MiSeq sequencing technology was used to analyze soil bacterial community diversity around an aging oil sludge in the Yellow River Delta. The alpha diversity index of soil bacterial community results (Ace, Chao, Shannon, and Simpson) determined that bacterial community diversity sampling within the scope of a 20 cm
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Enhancement of Organic Matter Removal in an Integrated Biofilm-Membrane Bioreactor Treating High-Salinity Wastewater. Archaea (IF 2.4) Pub Date : 2018-08-29 Yan Yang,Zhiyu Shao,Jun Du,Qiang He,Hongxiang Chai
High salinity can strongly inhibit microbial activity and decrease the sedimentation ability of activated sludge. The combination of biofilm and membrane bioreactor is a practical approach towards effective removal of pollutants and low fouling rate. An integrated biofilm-membrane bioreactor (BMBR) treating mustard tuber wastewater was investigated. An average COD removal efficiency of 94.81% and ammonium
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Comparative Genomic Analysis of Members of the Genera Methanosphaera and Methanobrevibacter Reveals Distinct Clades with Specific Potential Metabolic Functions. Archaea (IF 2.4) Pub Date : 2018-08-19 Anja Poehlein,Dominik Schneider,Melissa Soh,Rolf Daniel,Henning Seedorf
Methanobrevibacter and Methanosphaera species represent some of the most prevalent methanogenic archaea in the gastrointestinal tract of animals and humans and play an important role in this environment. The aim of this study was to identify genomic features that are shared or specific for members of each genus with a special emphasis of the analysis on the assimilation of nitrogen and acetate and
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Autohydrogenotrophic Denitrification Using the Membrane Biofilm Reactor for Removing Nitrate from High Sulfate Concentration of Water. Archaea (IF 2.4) Pub Date : 2018-08-05 Yanhao Zhang,Haohan Zhang,Zhibin Zhang,Yuchen Wang,Taha Marhaba,Jixiang Li,Cuizhen Sun,Wen Zhang
This study investigated the performance of an autohydrogenotrophic membrane biofilm reactor (MBfR) to remove nitrate from water with high sulfate concentrations. The results of simulated running showed that TN removal could be over than 98.8% with the maximum denitrification rate of 134.6 g N/m3 d under the conditions of the influent sulfate concentrations of 300 mg SO42−/l. The distribution ratio
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Bioaugmentation with Mixed Hydrogen-Producing Acetogen Cultures Enhances Methane Production in Molasses Wastewater Treatment. Archaea (IF 2.4) Pub Date : 2018-08-01 Shuo Wang,Jianzheng Li,Guochen Zheng,Guocheng Du,Ji Li
Hydrogen-producing acetogens (HPA) have a transitional role in anaerobic wastewater treatment. Thus, bioaugmentation with HPA cultures can enhance the chemical oxygen demand (COD) removal efficiency and CH4 yield of anaerobic wastewater treatment. Cultures with high degradation capacities for propionic acid and butyric acid were obtained through continuous subculture in enrichment medium and were designated
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Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila. Archaea (IF 2.4) Pub Date : 2018-07-17 Nina Lackner,Anna Hintersonnleitner,Andreas Otto Wagner,Paul Illmer
Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO2) for catabolic and anabolic metabolism was not proven until now. Here, we show that M. thermophila used CO2 to perform hydrogenotrophic methanogenesis in the presence as well as in the absence of methanol. During incubation with hydrogen, the methanogen
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Dynamics of Archaeal and Bacterial Communities in Response to Variations of Hydraulic Retention Time in an Integrated Anaerobic Fluidized-Bed Membrane Bioreactor Treating Benzothiazole Wastewater. Archaea (IF 2.4) Pub Date : 2018-04-29 Yue Li,Qi Hu,Da-Wen Gao
An integrated anaerobic fluidized-bed membrane bioreactor (IAFMBR) was investigated to treat synthetic high-strength benzothiazole wastewater (50 mg/L) at a hydraulic retention time (HRT) of 24, 18, and 12 h. The chemical oxygen demand (COD) removal efficiency (from 93.6% to 90.9%), the methane percentage (from 70.9% to 69.27%), and the methane yield (from 0.309 m3 CH4/kg·CODremoved to 0.316 m3 CH4/kg·CODremoved)
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Characterization of Microbial Communities in Pilot-Scale Constructed Wetlands with Salicornia for Treatment of Marine Aquaculture Effluents. Archaea (IF 2.4) Pub Date : 2018-04-29 Xiaona Ma,Xingqiang Song,Xian Li,Songzhe Fu,Meng Li,Ying Liu
Microorganisms play an essential role in the performance of constructed wetlands (CWs) for wastewater treatment. However, there has been limited discussion on the characteristics of microbial communities in CWs for treatment of effluents from marine recirculating aquaculture systems (RAS). This study is aimed at characterizing the microbial communities of pilot-scale CWs with Salicornia bigelovii for
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Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348T. Archaea (IF 2.4) Pub Date : 2018-03-05 Matías Rivero,Constanza Torres-Paris,Rodrigo Muñoz,Ricardo Cabrera,Claudio A Navarro,Carlos A Jerez
Polyphosphates (PolyP) are linear polymers of orthophosphate residues that have been proposed to participate in metal resistance in bacteria and archaea. In addition of having a CopA/CopB copper efflux system, the thermoacidophilic archaeon Metallosphaera sedula contains electron-dense PolyP-like granules and a putative exopolyphosphatase (PPXMsed, Msed_0891) and four presumed pho84-like phosphate
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Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane. Archaea (IF 2.4) Pub Date : 2017-12-17 Wei-Yu Chen,Lucia Kraková,Jer-Horng Wu,Domenico Pangallo,Lenka Jeszeová,Bing Liu,Hidenari Yasui
Tetramethylammonium-degrading methanogenic consortia from a complete-mixing suspended sludge (CMSS) and an upflow anaerobic sludge blanket (UASB) reactors were studied using multiple PCR-based molecular techniques and shotgun proteomic approach. The prokaryotic 16S rRNA genes of the consortia were analyzed by quantitative PCR, high-throughput sequencing, and DGGE-cloning methods. The results showed
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A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens. Archaea (IF 2.4) Pub Date : 2017-11-19 Feng Long,Liangliang Wang,Boguslaw Lupa,William B Whitman
Many hydrogenotrophic methanogens use either H2 or formate as the major electron donor to reduce CO2 for methane production. The conventional cultivation of these organisms uses H2 and CO2 as the substrate with frequent replenishment of gas during growth. H2 is explosive and requires an expensive gassing system to handle safely. Formate is as an ideal alternative substrate from the standpoints of both
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Expression, Purification, and Characterization of (R)-Sulfolactate Dehydrogenase (ComC) from the Rumen Methanogen Methanobrevibacter millerae SM9. Archaea (IF 2.4) Pub Date : 2017-11-06 Yanli Zhang,Linley R Schofield,Carrie Sang,Debjit Dey,Ron S Ronimus
(R)-Sulfolactate dehydrogenase (EC 1.1.1.337), termed ComC, is a member of an NADH/NADPH-dependent oxidoreductase family of enzymes that catalyze the interconversion of 2-hydroxyacids into their corresponding 2-oxoacids. The ComC reaction is reversible and in the biosynthetic direction causes the conversion of (R)-sulfolactate to sulfopyruvate in the production of coenzyme M (2-mercaptoethanesulfonic
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Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes. Archaea (IF 2.4) Pub Date : 2017-11-02 Lena Kröninger,Jacqueline Gottschling,Uwe Deppenmeier
DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. Methanomassiliicoccus luminyensis is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H2 and a growth yield of 2.4 g dry weight/mol CH4. M. luminyensis also uses methylamines + H2 (monomethylamine
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Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius. Archaea (IF 2.4) Pub Date : 2017-10-31 Shoji Suzuki,Norio Kurosawa
Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius, but repeated gene knockout based on PCR tailing has been limited due to lack of a genetic marker system
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Archaeal Diversity and CO2 Fixers in Carbonate-/Siliciclastic-Rock Groundwater Ecosystems. Archaea (IF 2.4) Pub Date : 2017-06-13 Cassandre Sara Lazar,Wenke Stoll,Robert Lehmann,Martina Herrmann,Valérie F Schwab,Denise M Akob,Ali Nawaz,Tesfaye Wubet,François Buscot,Kai-Uwe Totsche,Kirsten Küsel
Groundwater environments provide habitats for diverse microbial communities, and although Archaea usually represent a minor fraction of communities, they are involved in key biogeochemical cycles. We analysed the archaeal diversity within a mixed carbonate-rock/siliciclastic-rock aquifer system, vertically from surface soils to subsurface groundwater including aquifer and aquitard rocks. Archaeal diversity
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An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis. Archaea (IF 2.4) Pub Date : 2017-05-28 Yang Lü,Hua Lu,Shiwei Wang,Jing Han,Hua Xiang,Cheng Jin
A 1.1 × 106 Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC33960 with a production of 30 mg L−1 when grown in AS-168 medium, which mainly composed of mannose and galactose with a small amount of glucose in a molar ratio of 55.9 : 43.2 : 0.9. Two glycosyltransferase genes (HAH_1662 and HAH_1667) were identified to be responsible for synthesis
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The Draft Genome of the Non-Host-Associated Methanobrevibacter arboriphilus Strain DH1 Encodes a Large Repertoire of Adhesin-Like Proteins. Archaea (IF 2.4) Pub Date : 2017-05-28 Anja Poehlein,Rolf Daniel,Henning Seedorf
Methanobrevibacter arboriphilus strain DH1 is an autotrophic methanogen that was isolated from the wetwood of methane-emitting trees. This species has been of considerable interest for its unusual oxygen tolerance and has been studied as a model organism for more than four decades. Strain DH1 is closely related to other host-associated Methanobrevibacter species from intestinal tracts of animals and
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Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1. Archaea (IF 2.4) Pub Date : 2017-04-24 Thien-Hoang Ho,Kim-Hung Huynh,Diem Quynh Nguyen,Hyunjae Park,Kyoungho Jung,Bookyo Sur,Yeh-Jin Ahn,Sun-Shin Cha,Lin-Woo Kang
Thermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5′-phosphate- (PLP-) dependent enzyme of cysteine
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Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication. Archaea (IF 2.4) Pub Date : 2017-03-01 Yuchen Wang,Beibei Chen,Linshan Sima,Mengzhuo Cao,Xiangdong Chen
Haloarchaeon Natrinema sp. J7, the first reported archaeon harboring both plasmid and chromosome-based temperate viruses, is a useful model for investigating archaeal virus-host and virus-virus interactions. However, the lack of genetic tools has limited such studies. On the basis of the automatically replicating sequences of the J7 chromosome and the pyrF marker, we constructed seven vectors, six
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Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius. Archaea (IF 2.4) Pub Date : 2017-01-23 Konrad H Engelhardt,Shashank Reddy Pinnapireddy,Elias Baghdan,Jarmila Jedelská,Udo Bakowsky
Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods. The purified lipid fractions polar lipid fraction E (PLFE) and hydrolysed glycerol-dialkyl-nonitol tetraether (hGDNT) differ in their structures, charge, size
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Reverse Methanogenesis and Respiration in Methanotrophic Archaea. Archaea (IF 2.4) Pub Date : 2017-01-05 Peer H A Timmers,Cornelia U Welte,Jasper J Koehorst,Caroline M Plugge,Mike S M Jetten,Alfons J M Stams
Anaerobic oxidation of methane (AOM) is catalyzed by anaerobic methane-oxidizing archaea (ANME) via a reverse and modified methanogenesis pathway. Methanogens can also reverse the methanogenesis pathway to oxidize methane, but only during net methane production (i.e., “trace methane oxidation”). In turn, ANME can produce methane, but only during net methane oxidation (i.e., enzymatic back flux). Net
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Genome-Scale Metabolic Modeling of Archaea Lends Insight into Diversity of Metabolic Function. Archaea (IF 2.4) Pub Date : 2017-01-04 ShengShee Thor,Joseph R Peterson,Zaida Luthey-Schulten
Decades of biochemical, bioinformatic, and sequencing data are currently being systematically compiled into genome-scale metabolic reconstructions (GEMs). Such reconstructions are knowledge-bases useful for engineering, modeling, and comparative analysis. Here we review the fifteen GEMs of archaeal species that have been constructed to date. They represent primarily members of the Euryarchaeota with
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Marine Subsurface Microbial Community Shifts Across a Hydrothermal Gradient in Okinawa Trough Sediments. Archaea (IF 2.4) Pub Date : 2016-12-20 Leah D Brandt,Christopher H House
Sediments within the Okinawa back-arc basin overlay a subsurface hydrothermal network, creating intense temperature gradients with sediment depth and potential limits for microbial diversity. We investigated taxonomic changes across 45 m of recovered core with a temperature gradient of 3°C/m from the dynamic Iheya North Hydrothermal System. The interval transitions sharply from low-temperature marine
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Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters. Archaea (IF 2.4) Pub Date : 2016-12-15 Ayrat M Ziganshin,Elvira E Ziganshina,Sabine Kleinsteuber,Marcell Nikolausz
Comparative analysis of methanogenic archaea compositions and dynamics in 11 laboratory-scale continuous stirred tank reactors fed with different agricultural materials (chicken manure, cattle manure, maize straw, maize silage, distillers grains, and Jatropha press cake) was carried out by analysis of the methyl coenzyme-M reductase -subunit (mcrA) gene. Various taxa within Methanomicrobiales, Methanobacteriaceae
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Volatile Fatty Acids Production from Codigestion of Food Waste and Sewage Sludge Based on β-Cyclodextrins and Alkaline Treatments. Archaea (IF 2.4) Pub Date : 2016-12-14 Xue Yang,Xiang Liu,Si Chen,Guangmin Liu,Shuyan Wu,Chunli Wan
Volatile fatty acids (VFAs) are preferred valuable resources, which can be produced from anaerobic digestion process. This study presents a novel technology using β-cyclodextrins (β-CD) pretreatment integrated alkaline method to enhance VFAs production from codigestion of food waste and sewage sludge. Experiment results showed that optimized ratio of food waste to sewage sludge was 3 : 2 because it
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The Distribution Pattern of Sediment Archaea Community of the Poyang Lake, the Largest Freshwater Lake in China. Archaea (IF 2.4) Pub Date : 2016-12-13 Yantian Ma,Fangpeng Liu,Zhaoyu Kong,Jianhua Yin,Wenbo Kou,Lan Wu,Gang Ge
Archaea plays an important role in the global geobiochemical circulation of various environments. However, much less is known about the ecological role of archaea in freshwater lake sediments. Thus, investigating the structure and diversity of archaea community is vital to understand the metabolic processes in freshwater lake ecosystems. In this study, sediment physicochemical properties were combined