Abstract Sequencing and analysis of the genome of the chloramphenicol-resistant strain Acinetobacter lwoffii VS15, isolated from permafrost, revealed a circular plasmid 11 964 bp in length, designated pAL-WVS1.4. Apart from the genes supporting the plasmid maintenance and mobilization, pALWVS1.4 contains the cflA gene encoding the membrane protein of the MFS transporter family. Close homologues of

Abstract — The summer of 2015 was characterized by a large number of forest fires along the coastline of Lake Baikal. The concentrations of all analyzed polycyclic aromatic hydrocarbons (PAHs) in the surface microlayer of Lake Baikal during wildfires in August 2015 were significantly increased compared to August 2017, when there were fewer fires. Among the PAHs, naphthalene, phenanthrene and pyrene

Abstract— Planctomycetes are a phylogenetic group of bacteria with complex cell organization, large genomes and a wide spectrum of genome-encoded secondary metabolites. As suggested by analysis of available planctomycete genomes, these bacteria represent a promising source of potentially novel biologically active compounds. The number of cultured planctomycetes, however, remains limited. This study

Abstract The focus of present study was to isolate and characterize toluene metabolizing bacteria and evaluate their bioremediation potential. During present study bacteria were isolated from the tannery effluents using selective enrichment and serial dilution methods. The growth behavior, toluene removal efficiencies, biochemical and molecular characteristics and antibiotic resistance profiles of

Abstract— Effect of abiotic (composition of drip and fracture water) and biotic factors (composition and activity of microbial complexes) on biofilm formation in the Proshchal’naya karst cave (Far East, Russia) is reported. The effect of natural infiltration water on the structure of cultured microbial complexes and on the elemental composition of the moonmilk speleothem formed on the walls of the

Abstract— Yeast number and species diversity in urban soils characterized by various intensities of anthropogenic impact in the southern cities of Russia (Krasnodar, Maykop, Simferopol, and Sochi) was investigated. The soils of botanical gardens and the reference soils in the vicinities of cities were used for comparison to reveal the degree of anthropogenic impact. The maximum yeast number was found

Abstract— The goal of the present work was to study the oxidation of sulfide mineral pyrrhotite by members of microbial groups predominant in biohydrometallurgical processes (mixotrophic iron- and sulfur-oxidizing bacterium Sulfobacillus thermosulfidooxidans strain VKMV 1269T, autotrophic sulfur-oxidizing bacterium Acidithiobacillus caldus strain MBC-1, and heterotrophic iron- and sulfur-oxidizing

Abstract— The composition of the microbial community formed in the course of long-term (over 60 days) continuous cultivation of an obligate methanotroph Methylococcus sp. Concept-8 on natural gas in a 50-L bioreactor at 42°C and pH 5.6 was monitored. Stable growth with high optical density of the culture (average OD540 = 13.5), specific growth rate of 0.2 h–1, and biomass yield of 4.0–4.5 g dry matter

Abstract— Increasing interest in microbial communities of Arctic and Antarctic extreme environments, their taxonomic diversity, and forms and mechanisms of adaptation of inhabitants of Antarctic biotopes to extreme conditions determined the goal of the present work. Next generation sequencing of the 16S rRNA gene fragments was used to determine the taxonomic structure of microbial communities in soil

Abstract— This is the first report on the application of high-throughput sequencing of the 16S rRNA gene fragments for investigation of bacterioplankton diversity in two largest and relatively young reservoirs of Mongolia—Taishir, formed on the Zavkhan River, and Durgun, formed on the Chono Harayh Channel. A total of 145 phylotypes (with a cluster distance of 0.03) belonging to 18 phyla were identified

Abstract— Although dissimilatory sulfate reduction (DSR) is an important microbial process in subterranean aquifers, its geochemical consequences in this ecosystem remain insufficiently studied. The absence of data on the process rate under in situ conditions prevents quantitative estimation of the sulfur reservoir. This research is aimed at investigation of microbial sulfate reduction in subterranean

Abstract Nine chitin-degrading isolates of moderately thermophilic bacteria with temperature optimum for growth and chitin destruction at 50–55°C were isolated from soils of the New Athos Cave (Abkhazia). Four most active bacterial cultures with chitinase production yields 0.11–0.28 U mL–1 (at 48°C) were screened from seven isolates forming aerial mycelium. Selected isolates were identified as Laceyella

Abstract A study and comparative analysis of production characteristics of bacterio- and phytoplankton in Avacha Bay and in the southern region of the Far Eastern State Marine Biosphere Reserve (FEMBR) in Posyet Bay was carried out in summer, 2017. Bacterial production in the surface samples from these basins was similar, varying from 0.28 to 2.3 mg C/(day m3) for Avacha Bay and from 0.22 to 2.0 mg

Abstract— The composition of the microbiome of acid drainage water of a quarry used for the mining of brown coal was studied. The water had a pH of 5.15 and contained 1.4 g/L of sulfate. Unlike acid mine drainage associated with mining of sulfide ores, the content of iron and heavy metals was low. Molecular analysis of the microbial community was performed by high-throughput sequencing of the 18S and

Abstract Current study reports isolation of newly isolated slight halophilic bacteria which can utilize 2,4,6-trinitrotoluene (TNT) as a sole nitrogen source, leading to its detoxification. Among 100 halo(alkali)tolerant and halo(alkali)philic strains were isolated from TNT-contaminated soil, two slight halophilic alkalitolerant strains showed remarkable ability to quickly remove the different concentrations

Abstract— The structures of exo-oligosaccharides produced by a symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum bv. viciae VF39 were determined using the methods of mass spectrometry and HCD fragmentation of ions. Exo-oligosaccharides were shown to be monomers, dimers and trimers of an octasaccharide repeating unit of the high-molecular-weight acidic exopolysaccharide. The major fraction

AbstractMicromycetes can precipitate metals in the form of insoluble oxalates, thus participating in metal removal from geochemical cycling and in secondary mineral formation. Metal precipitation has a practical application for bioleaching and bioremediation. Micromycetes from the genus Aspergillus are used as model organisms to study metal precipitation. All known biomineralization processes carried

AbstractThis is the first report on ability of bacteria of the genus Azospirillum to decolorize synthetic dyes. Screening revealed A. brasilense strain SR80 to exhibit the highest decolorization efficiency. The discoloration degree was as high as 50% for remazol brilliant blue and over 90% for malachite green. The cultivation conditions affecting the efficiency of degradation of the studied dyes were

Abstract—Molecular genetic study of Saccharomyces yeasts of different species was carried out using molecular karyotyping and comparative analysis of a number of nuclear and mitochondrial genes. Multigene phylogenetic analysis showed that the recently described species S. jurei was phylogenetically closest to S. mikatae, while S. bayanus and S. arboricola were the most divergent species within the

Adaptive response (changes in the composition of osmolytes and membrane lipids) of the mycelial fungus Aspergillus niger to combinatorial action of oxidative and heat (or osmotic) shocks was studied. Oxidative shock was found to cause no significant changes in the composition of osmolytes. A combination of oxidative shock with other stressors was shown to suppress their adaptive responses, such as

AbstractThe application of restriction analysis of amplification products of the genes rpoC and alkB, encoding the synthesis of the DNA-dependent RNA polymerase β'-subunit and alkane-1-monooxygenase, respectively, for species identification of R. erythropolis and R. qingshengii was studied. Restriction of the rpoC amplicons (1236 bp) by the enzymes AatII and SacI resulted in formation of the fragments

Abstract—Microbiological processes occurring in the course of controlled thermophilic composting of dehydrated wastewater sludge anaerobically digested in a reactor at the Lyubertsy waste treatment plant (Moscow, Russia) were studied. Dynamics of the concentrations of nitrogen species in the presence of microorganisms of the nitrogen cycle (ammonifying, nitrifying, denitrifying, and nitrogen-fixing)

AbstractThis is the first report on biofilm formation by Micrococcus luteus C01 in model systems stimulated by epinephrine. In the presence of 900 pg/mL of epinephrine in the medium, a 52.3% increase in the metabolic activity of biofilm bacteria compared to the control (without epinephrine). However, after 72 h of growth, the total amount of biofilm biomass in the presence of epinephrine increased

AbstractPatterns of formation of three-species biofilms on the surface of an LDPE-based composite material were studied using a mixture of pure planktonic cultures of microorganisms capable of degrading this polymer. This is the first report of the role of the ascomycete Yarrowia lipolytica as a primary colonizer in this system, forming aggregates with gram-negative bacteria Pseudomonas aeruginosa

AbstractBiogenic sulfide production in oilfields results in lower quality of oil and gas and in corrosion of oil-producing equipment. Bactericidal agents are not used at the Karazhanbas oilfield (Kazakhstan), since sulfate concentrations in both injected and formation water are low or zero. However, increasing corrosion of steel equipment and sulfide release into formation water were observed in the

AbstractAcidobacteria are one of the widespread and numerically abundant groups of prokaryotes in soils and peatlands. The functional potential of these microorganisms remains poorly understood due to the limited number of cultured and characterized representatives. Acidisarcina polymorpha SBC82 is the only acidobacterium for which the ability to use chitin as a source of nitrogen and carbon has been

AbstractThe goal of the present work was to study the bioleaching of chalcopyrite (CuFeS2), enargite (Cu3AsS4), and tennantite (Cu12As4S13) by pure and mixed cultures of moderately thermophilic microorganisms belonging to the groups predominant in technological processes (Acidithiobacillus caldus MBC-1, Sulfobacillus thermosulfidooxidans SH-1, and Acidiplasma sp. MBA-1) under different conditions (temperature

AbstractMarine actinobacteria particularly from marine environments are believed to be inexhaustible sources of biologically active molecules for biomedical and industrial applications. We isolated 126 strains of marine actinomycetes from marine invertebrates such as corals, sponges and gastropod molluscs from Daya Bay and Nansha Islands. The chemical diversity of the metabolites from the isolated

AbstractCertain endophytic fungi isolated from Torreya grandis can produce similar unsaturated lipid of high economic value as its host plants. However, the lipid yield produced by endophytic fungi is very low. Acetyl-CoA carboxylase (ACC) is one of the key enzymes responsible for fatty acid synthesis. In this study, the impact of ACC on lipid yield of endophytic fungi were analyzed and the inhibitory

AbstractPersisters (P) are the cells surviving, but not reproducing in the presence of lethal doses of antibiotics (AB). These are the cells of a scarce (0.001–1.0%) subpopulation, emerging due to a phenotypic transition, which may be stochastic or induced by stress impacts. Growing in fresh medium, P reproduce the structure of the parent population, which is considered the cause of: (a) recurrent

AbstractInvestigation of free-living nitrogen-fixing bacteria from the salinated arid soil ecosystems is important for developing novel biological solutions for dryland agriculture. The present work reports isolation, screening and identification of two strains from salt-affected soils of Latakia (Syria) and Aral Sea basin (Uzbekistan): Gracilibacillus sp. Str01 and Halomonas sp. Str02. Nitrogenase

Abstract—Denitrification is the major process of nitrate reduction to dinitrogen (N2). This is the first report on detection of the nirK type denitrifying bacteria in the epilithic biofilms of the Lake Baikal littoral zone. The highest species diversity was revealed in microbial communities of the biofilms collected in the area of the Bol’shoe Goloustnoe settlement and Severobaikal’sk City, while those

AbstractAccording to 16S rRNA gene- or genome-based phylogeny, magnetotactic bacteria (MTB) belong to diverse taxonomic groups. Here we analyzed the diversity of MTB in a sample taken from the freshwater Lake Beloe Bordukovskoe near Moscow, Russia, by using molecular identification based on sequencing of the 16S rRNA gene and mamK, a specific marker gene for these bacteria. A protein encoded by the

Abstract—Iron- and sulfur-oxidizing auto-, mixo- and heterotrophic bacteria and archaea dominate in the bioleaching of pyrite from sulfide ores and concentrates both in natural conditions and in biohydrometallurgical processes. The goal of the present work was to study the oxidation of pyrite, the most widespread sulfide mineral, by representatives of microbial groups predominant in biohydrometallurgical

Abstract—Microbial degradation of lignocellulose and soil organic matter is an important process controlling CO2 flow into the atmosphere, which is mostly carried out by fungi. The alkali-soluble fraction, which is represented mostly by the so-called humic acids (HA), dark-colored compounds of phenolic nature, constitute ~30‒70% of soil Corg. This is the first report on efficient degradation of soil

AbstractThe candidate phylum Riflebacteria was described based on analysis of genomes assembled from the metagenomes of various anaerobic ecosystems; however, to date, no member of Riflebacteria has been isolated in a pure culture. In this work, the genome of a new member of Riflebacteria was obtained from the metagenome of the microbial community of a deep subsurface thermal aquifer in Western Siberia

Abstract—The hydrochemical water composition and the mineralogical composition of microbial mats and bottom sediments were determined for the ephemeral saline soda lake Gudzhirganskoe (Barguzin Valley, Baikal Rift zone). Analysis of the 16S rRNA gene amplicons was applied to investigation of microbial diversity in several biotopes: brine (1 sample), sediments (3), and microbial mats (3). During sampling

Abstract—The review clarifies the mechanisms of H2 production by green microalgae and the physiological role of the [FeFe] hydrogenase in adaptation of these organisms to varying environmental conditions, primarily to anaerobiosis. The work is focused at interrelationships and mutual regulation between biological hydrogen production, photosynthetic electron transport, fermentation and oxygen uptake

Abstract—Regardless of cultivation conditions, two types of LH2 complexes are always assembled in photosynthetic membranes of Thiorhodospira (T.) sibirica: short-wave B800−830 and long-wave B800−850. The present work studied the effect of qualitative and quantitative content of carotenoids on assembling of these complexes. Diphenylamine (DPA), an inhibitor of carotenoid biosynthesis, was used to decrease

Abstract—The complete genome sequence of a thermotolerant obligate methanotroph Methylococcus sp. Concept-8 was determined and analyzed. This strain was obtained by long-term storage, selection, and purification of an industrial culture of Methylococcus capsulatus BSV-874, which was used for methane-based protein biosynthesis in the Soviet Union. The size of the Concept-8 genome is 3.46 Mb. Genome

Abstract—The effect of 25Mg, a magnetic magnesium isotope, on the sensitivity of Escherichia coli K12 TG1 to quinolone/fluoroquinolone (nalidixic acid and ciprofloxacin) and aminoglycoside antibiotics (amikacin and tobramycin) was found. In the case of fluoroquinolones, determination of antibiotic sensitivity by the disc diffusion method revealed the 7‒14% larger growth inhibition zones on the medium

Abstract—The results of field studies of the species composition and productive parameters (biomass, photosynthesis rate, chlorophyll a content, and assimilation numbers) of microalgae in the plankton and the interstitial icy waters of Lake Baikal southern littoral area obtained in March 2018 are presented. A comprehensive study of the ice and phytoplankton algae showed that their productive activity

Abstract—The genes encoding carbonic anhydrase (CA) were found in all anoxygenic purple bacteria. The genes of α- and β-CA were found in purple nonsulfur bacteria of the class Alphaproteobacteria:Rhodospirillum rubrum, Rhodospirillum fulvum, Rhodoblastus acidophilus, and Rhodopseudomonas palustris. The alphaproteobacteria Rhodomicrobium vannielii, Blastochlorisviridis, Rhodobacter sphaeroides, Rhodobacter

Abstract—Molecular genetic study of 22 methanol-assimilating Komagataella strains maintained at the UCDFST collection (United States) was carried out. Multigene phylogenetic analysis revealed that strains previously assigned to “Pichia pastoris” based on the standard taxonomic tests actually belong to four different species: K. pastoris, K. phaffii, K. mondaviorum, and K. ulmi. Seven Komagataella species

AbstractPhages are considered the most abundant and diverse biological entities on Earth. Although many phages have been isolated, most of this diversity remains completely unexplored. Recent advances in genome sequencing, however, have provided unprecedented glimpses into the virosphere. The lytic cold-active Siphoviridae bacteriophage VW6S, infecting the host Pseudomonas fluorescens W-6, was isolated

Abstract—A bacterial strain CT3 with a unique ability to degrade anthropogenic toxic compounds ε-caprolactam, toluene, and meta-xylene as sole sources of carbon and energy was isolated from the activated sludge of a municipal wastewater treatment plant. Based on sequencing and phylogenetic analysis of its 16S rRNA gene, the isolate was identified as a Pseudomonas putida strain. P. putida strain СТ3

Abstract—Metagenomics of the viromes associated with symbiotic microbial communities is a rapidly expanding field of research. Most of the relevant studies deal with the viral communities of human or animal feces. The novel technology of long-reads sequencing from Oxford Nanopore that became available during the last years provides outstanding new possibilities for research of uncultured viral communities

Abstract—The composition of rhizosphere microbial communities was studied for different species and varieties of agricultural gramineous plants (rye and wheat) grown in the soils contrasting in a number of agrochemical parameters. The data on analysis of the rhizosphere microbial communities obtained by sequencing of the V4 variable region of the 16S rRNA gene and on the ion and mass spectral composition

Abstract—This is the study of efficiency of anaerobic reject water treatment and of changes in the relative abundance of the major microbial groups involved in nitrogen removal at increase in nitrogen and COD (chemical oxygen demand) load. A two-reactor laboratory system carried out anammox and partial nitrification, with the biomass immobilized on a brush carrier and water recycling. Nitrogen concentration

Abstract—The possibility of singlet oxygen generation in five species of photosynthetic bacteria under irradiation with light was studied using the Singlet Oxygen Sensor Green (SOSG) dye that, when singlet oxygen is bound, is transformed into a fluorescent endoperoxide form. Irradiation was carried out using light of different spectral composition: red (absorption of bacteriochlorophyll, BChl) and

Abstract—Anaerobic digestion of municipal and other organic waste is a microbial process for conversion of complex organic substances to biogas (a renewable energy source) comprising a mixture of methane and CO2, and a stabilized sludge, which may be used as an organic fertilizer. Diverse groups of the methanogenic microbial community degrade complex organic compounds into simple fermentation products

Abstract—Relationship between symbiotic yeast and Diptera insects are studied mainly on fruit flies (Drosophilidae). Evolutionary experiments showed that changes in the composition of the yeast microbiota vectored by flies in their gut and on the body surface contributes to the adaptation of laboratory Drosophila populations to the high-salt growth medium. Although saline substrates are not commonly

Abstract—Surface-enhanced Raman scattering of bioorganic compounds close to silver nanoparticles may be used for species identification of microbial colonies. The preparations of silver nanoparticles sol with a single microbial colony (strains Escherichia coli 376/2, Staphylococcus aureus 25923, Pseudomonas aeruginosa ATCC 27583, and Bacillus subtilis 534) exhibited the individual Raman spectra, in

AbstractPhylogenetic analysis of the 16S rRNA gene sequences showed that the type species of the genus Methylarcula, Methylarculamarina h1T was closely related to Paracoccus saliphilus DSM 18447T with high sequence similarity (97.8%). The genome of Methylarcula marina h1T was sequenced and its average nucleotide identity (ANI) and in silico DNA–DNA hybridization with Paracoccus type strains were 72

Abstract—The pmoA gene number is considered as a soil microbiological parameter indicating abundance and potential activity of methanotrophic bacteria. The efficiency of this parameter for prediction and modeling of the real soil methane consumption rates remains an open issue. In the current study rate of methane oxidation by soil at its ambient concentration and the number of the pmoA genes determined

Abstract—Molecular genetic techniques (FISH and metabarcoding) were used to investigate comparative biodiversity in the prokaryotic complex of soil microcosms of gray forest, chestnut, and chernozem soils before and after oil pollution. At the level of high-rank taxonomic units, the structure of prokaryotic communities from different soil types was similar. In oil-polluted microcosms microbial diversity

AbstractNonsteroidal anti-inflammatory drugs (NSAIDs) are a group of pharmaceuticals widely used for medical and veterinary purposes. NSAIDs have been found in nanograms and micrograms in soil, wastewater, surface water, groundwater, sediments, snow, Antarctic ice, and drinking water. Despite negligible detectable amounts in the environment, NSAIDs have chronic ecotoxic effects on the biotic components

Abstract—We have previously proposed a highly efficient technology for metal recovery from low-grade zinc sulfide concentrate by two-step treatment including ferric iron leaching and biooxidation of the produced ferrous iron to ferric iron (an oxidizer for sulfide minerals). The present work proposes joint biooxidation of the products of ferric leaching of the low-grade zinc concentrate: a solution

Abstract—Specific amplification of nucleic acids is a convenient and quick alternative to the culture-based method of detecting bacterial cells. However, conventional PCR and other amplification reactions can not differentiate between live bacteria and dead or dormant ones, and are also capable of amplifying DNA that persists for a long time and in a cell-free state. Several methods have been developed

Abstract—Dissolved organic matter (DOM) release by anoxygenic phototrophic bacteria belonging to diverse taxonomic groups is a ubiquitous process, resulting in 5–20% of the CO2 fixed by photosynthesis leaving the cell. The green photosynthetic bacterium Chlorobaculum limnaeum releases ~10% of the carbon assimilated during autotrophic СО2 fixation. Extracellular DOM release by Cba. limnaeum is a normal