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AL DISCRETO LETTORE: A short-list on Public Health Microbiology in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-03-09 Anton Aebischer, Annette Mankertz, Guido Werner, Sebastian Suerbaum
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Preparation and immunogenicity evaluation of C-HapS-P6 fusion protein vaccine against nontypeable Haemophilus influenzae in mice Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-03-06 Nan Hu, Weifeng Li, Zihong Zhao, Yueli Chang, Cai Wang, Yutuo Zhang
Nontypeable (NTHi) is the dominant pathogen in several infectious diseases. Currently the use of antibiotics is the main intervention to prevent NTHi infections, however with the emergence of drug resistant strains, it has compromised the treatment of respiratory infections with antibiotics. Therefore there is an urgent need to develop a safe and effective vaccine to prevent NTHi infections. We investigate
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Public Health Microbiology: Recent developments under Robert Koch’s genius loci Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-22 Annette Mankertz, Lars Schaade
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Talaromyces marneffei endocarditis initially detected by Next Generation Sequencing: A case report Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-21 Youqi Ji, Bingqian Zhuo, Tao Jiang, Mengyuan Chen, Wei Xu, Yuhuan Shen, Dongqing Cheng, Yumei Ge
() is a thermal dimorphic fungus, which can cause lung or blood stream infection in patients, often life-threatening. However, endocarditis caused by has not been reported. For elderly patients with implanted cardiac devices or artificial valves, the prevention and treatment of infective endocarditis should not be ignored. This is a descriptive study of a endocarditis by joint detection of cardiac
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Survival after cryptococcosis in Germany: A retrospective multicenter cohort study of patients diagnosed between 2004 and 2021 Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-16 Johanna Kessel, Anna-Catharina Rossaert, Tilman Lingscheid, Jan Grothe, Thomas Harrer, Christoph Wyen, Daniela Tominski, T. Bollinger, Anna Katharina Kehr, Sven Kalbitz, Christian Hoffmann, Oliver Cornely, Uwe Koppe, Christoph Stephan, Volker Rickerts
Cryptococcosis is the most prevalent fungal infection of the central nervous system worldwide. We performed a retrospective multicenter cohort study to gain insights into the epidemiology of cryptococcosis in Germany. We describe the use of diagnostic tests, clinical management and patient outcome. We included 64 patients with underlying HIV infection (55%) or other predispositions. Molecular typing
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Presence of hypervirulence-associated determinants in Klebsiella pneumoniae from hospitalised patients in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-08 Anika Wahl, Martin A. Fischer, Kathleen Klaper, Annelie Müller, Stefan Borgmann, Johannes Friesen, Klaus-Peter Hunfeld, Arkadius Ilmberger, Susanne Kolbe-Busch, Michael Kresken, Norman Lippmann, Christoph Lübbert, Matthias Marschner, Bernd Neumann, Niels Pfennigwerth, Michael Probst-Kepper, Jürgen Rödel, Marco H. Schulze, Andreas E. Zautner, Guido Werner, Yvonne Pfeifer
(.) is a ubiquitous Gram-negative bacterium and a common coloniser of animals and humans. Today, is one of the most persistent nosocomial pathogens worldwide and poses a severe threat/burden to public health by causing urinary tract infections, pneumonia and bloodstream infections. Affected by infections are mainly immunocompromised individuals and hospitalised patients. In recent years, a new type
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PASQUALE - a long-term partnership to improve hand hygiene and capacity building in infection prevention and control in the Faranah region of Guinea Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-05 Anna Borodova, Aziz Amadou Diallo, Rebekah Wood, Ousmane Tounkara, Carlos Rocha, Mouctar Bayo, Lena Landsmann, Mahamoud Sama Cherif, Matthias Borchert, Carolin Meinus, Ibrahima Nabé, Sekou Doumbouya, Kamis Mamadou Diallo, Mamadou Diallo, Mardjan Arvand, Sophie A. Müller
Across the globe, hand hygiene (HH) is promoted to fight the spread of healthcare associated infections. Despite multiple ongoing HH campaigns and projects, the healthcare associated infection rates remain high especially in low- and middle-income countries.
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Measles vaccination – An underestimated prevention measure: Analyzing a fatal case in Hildesheim, Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-03 Katharina Hueppe, Jan Ortman, Hannah Gauselmann, Sabine Santibanez, Annette Mankertz
Measles and rubella are targeted for elimination in the WHO region Europe. To reach the elimination goal, vaccination coverage of 95% must be achieved and sustained, the genotype information has to be provided for 80% of all outbreaks and transmission chains of a certain variant must not be detected for > 12 months. The latter information is collected at Germany’s National Reference Center Measles
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Integrated molecular, phenotypic and epidemiological surveillance of antimicrobial resistance in Neisseria gonorrhoeae in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-29 Kathleen Klaper, Hana Tlapák, Regina Selb, Klaus Jansen, Dagmar Heuer
Numbers of infections with Neisseria gonorrhoeae are among the top three sexually transmitted infections (STI) worldwide. In addition, the emergence and spread of antimicrobial resistance (AMR) in Neisseria gonorrhoeae pose an important public-health issue. The integration of genomic, phenotypic and epidemiological data to monitor Neisseria gonorrhoeae fosters our understanding of the emergence and
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Genomic surveillance of STEC/EHEC infections in Germany 2020 to 2022 permits insight into virulence gene profiles and novel O-antigen gene clusters Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-02-01 Angelika Fruth, Christina Lang, Tobias Größl, Thomas Garn, Antje Flieger
Shiga toxin-producing (STEC), including the subgroup of enterohemorrhagic (EHEC), are important bacterial pathogens which cause diarrhea and the severe clinical manifestation hemolytic uremic syndrome (HUS). Genomic surveillance of STEC/EHEC is a state-of-the-art tool to identify infection clusters and to extract markers of circulating clinical strains, such as their virulence and resistance profile
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Characteristics of two zoonotic swine influenza A(H1N1) viruses isolated in Germany from diseased patients Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-24 Alla Heider, Marianne Wedde, Viola Weinheimer, Stephanie Döllinger, Masyar Monazahian, Ralf Dürrwald, Thorsten Wolff, Brunhilde Schweiger
Interspecies transmission of influenza A viruses (IAV) from pigs to humans is a concerning event as porcine IAV represent a reservoir of potentially pandemic IAV. We conducted a comprehensive analysis of two porcine A(H1N1)v viruses isolated from human cases by evaluating their genetic, antigenic and virological characteristics. The HA genes of those human isolates belonged to clades 1C.2.1 and 1C
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Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-23 Benedikt Schrenker, Anna Zimmermann, Thorsten Koch, Grit Walther, Ronny Martin, Daniel Kampik, Oliver Kurzai, Johanna Theuersbacher
Purpose Fusarium keratitis is a severe infection of the anterior eye, frequently leading to keratoplasty or surgical removal of the affected eye. A major risk factor for infection is the use of contact lenses. Inadequate hygiene precautions and mold-growth permissive storage fluids are important risk factors for fungal keratitis. The aim of this study was to comparatively analyze contact lens storage
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Hub and Spoke: Next level in regional networks for infection prevention Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-26 Daniele Pantano, Alexander W. Friedrich
The threat of multidrug-resistant organisms (MDROs) and antimicrobial resistance (AMR) are real and increasing every day. They affect not only healthcare systems but also communities, causing economic and public health concerns. Governments must take action to tackle AMR and prevent the spread of MDROs and regional hubs have a critical role to play in achieving this outcome. Furthermore, bacteria have
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Vaccine-induced neutralizing antibodies bind to the H protein of a historical measles virus Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-20 Anne Zemella, Kerstin Beer, Franziska Ramm, Dana Wenzel, Ariane Düx, Kevin Merkel, Sebastien Calvignac-Spencer, Daniel Stern, Martin B. Dorner, Brigitte G. Dorner, Navena Widulin, Thomas Schnalke, Cornelia Walter, Anne Wolbert, Bernhard G. Schmid, Annette Mankertz, Sabine Santibanez
Measles is a highly contagious airborne viral disease. It can lead to serious complications and death and is preventable by vaccination. The live-attenuated measles vaccine (LAMV) derived from a measles virus (MV) isolated in 1954 has been in use globally for six decades and protects effectively by providing a durable humoral and cell-mediated immunity. Our study addresses the temporal stability of
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Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-18 Anna Maria Oschmann-Kadenbach, Christoph Schaudinn, Leonard Borst, Carsten Schwarz, Katharina Konrat, Mardjan Arvand, Astrid Lewin
Mycobacteroides abscessus is one of the most resistant bacteria so far known and causes severe and hard to treat lung infections in predisposed patients such as those with Cystic Fibrosis (CF). Further, it causes nosocomial infections by forming biofilms on medical devices or water reservoirs. An eye-catching feature of M. abscessus is the growth in two colony morphotypes. Depending on the presence
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Impact of the COVID-19 pandemic on norovirus circulation in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-18 Sonja Jacobsen, Mirko Faber, Britta Altmann, Andreas Mas Marques, C.-Thomas Bock, Sandra Niendorf
Human norovirus is a major cause of viral gastroenteritis in all age groups. The virus is constantly and rapidly changing, allowing mutations and recombination events to create great diversity of circulating viruses. With the start of the COVID-19 pandemic in 2020, a wide range of public health measures were introduced worldwide to control human-to-human transmission of SARS-CoV-2. In Germany, control
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Developing a next level integrated genomic surveillance: Advances in the molecular epidemiology of HIV in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-20 Kirsten Hanke, Vera Rykalina, Uwe Koppe, Barbara Gunsenheimer-Bartmeyer, Dagmar Heuer, Karolin Meixenberger
Advances in the molecular epidemiological studies of the Human Immunodeficiency Virus (HIV) at the Robert Koch Institute (RKI) by laboratory and bioinformatic automation should allow the processing of larger numbers of samples and more comprehensive and faster data analysis in order to provide a higher resolution of the current HIV infection situation in near real-time and a better understanding of
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A look at staphylococci from the ONE HEALTH perspective Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-18 Christiane Cuny, Franziska Layer-Nicolaou, Guido Werner, Wolfgang Witte
Staphylococcus aureus and other staphylococcal species are resident and transient multihost colonizers as well as conditional pathogens. Especially S. aureus represents an excellent model bacterium for the “One Health” concept because of its dynamics at the human-animal interface and versatility with respect to host adaptation. The development of antimicrobial resistance plays another integral part
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Accelerating SARS-CoV-2 genomic surveillance in a routine clinical setting with nanopore sequencing Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-18 Sergio Buenestado-Serrano, Marta Herranz, Álvaro Otero-Sobrino, Andrea Molero-Salinas, Cristina Rodríguez-Grande, Amadeo Sanz-Pérez, María José Durán Galván, Pilar Catalán, Roberto Alonso, Patricia Muñoz, Laura Pérez-Lago, Darío García de Viedma
Background SARS-CoV-2 genomic analysis has been key to the provision of valuable data to meet both epidemiological and clinical demands. High-throughput sequencing, generally Illumina-based, has been necessary to ensure the widest coverage in global variant tracking. However, a speedier response is needed for nosocomial outbreak analyses and rapid identification of patients infected by emerging VOCs
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Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-11 Yuguang Fu, Marianne Wedde, Sigrun Smola, Djin-Ye Oh, Thorsten Pfuhl, Jürgen Rissland, Michael Zemlin, Fidelis A. Flockerzi, Rainer M. Bohle, Andrea Thürmer, Susanne Duwe, Barbara Biere, Janine Reiche, Brunhilde Schweiger, Christin Mache, Thorsten Wolff, Georg Herrler, Ralf Dürrwald
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Pasteurella multocida strains of a novel capsular serotype and lethal to Marmota himalayana on Qinghai-Tibet plateau in China Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2024-01-05 Ran Duan, Dongyue Lyu, Shuai Qin, Junrong Liang, Wenpeng Gu, Qun Duan, Weiwei Wu, Deming Tang, Haonan Han, Xiaojin Zheng, Jinxiao Xi, Asaiti Bukai, Xinmin Lu, Peng Zhang, Dan Zhang, Meng Xiao, Huaiqi Jing, Xin Wang
Pasteurella multocida is a zoonotic pathogen causing serious diseases in humans and animals. Here, we report P. multocida from wildlife on China's Qinghai-Tibet plateau with a novel capsular serotype, forming a single branch on the core-genome phylogenetic tree: four strains isolated from dead Himalayan marmot (Marmota himalayana) and one genome assembled from metagenomic sequencing of a dead Woolly
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Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-12-19 Qiulei Zhang, Jingxiao Zhang, Yu Zhang, Yujie Sui, Yang Du, Longfei Yang, Yongjie Yin
The opportunistic fungal pathogen Candida albicans could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of C. albicans to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this study, we, for the first time, showed that patchouli alcohol (PA) can inhibit the growth of multiple
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Etiology of nosocomial infections in intensive care patients in German hospitals: An analysis of trends between 2008 and 2022 Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-12-22 Petra Gastmeier, Axel Kola, Frank Schwab, Michael Behnke, Christine Geffers
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Prolonged carriage of OXA-244-carbapenemase-producing Escherichia coli complicates epidemiological investigations Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-12-19 Felix Reichert, Simon Brinkwirth, Niels Pfennigwerth, Sebastian Haller, Lena Sophie Fritsch, Tim Eckmanns, Guido Werner, Sören Gatermann, Jörg B. Hans
The rapid increase of OXA-244-producing Escherichia coli, predominantly driven by genetically clustered isolates of sequence type (ST)38, has been observed in at least nine European countries, including Germany. However, the reasons for the spread of OXA-244-producing E. coli remain unclear. Here, we aim to evaluate the possibility of prolonged carriage. We identified a total of six different patients
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Past and present seroprevalence and disease burden estimates of Toxoplasma gondii infections in Germany: An appreciation of the role of serodiagnostics Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-12-01 Frank Seeber
Toxoplasmosis is one of the major foodborne parasitic diseases in Germany, with 49% of its population chronically infected with its causative agent, Toxoplasma gondii. Although the acute disease is usually benign in immunocompetent individuals, it is a threat for immunocompromised patients as well as for fetuses of seronegative mothers. As a result of infection, congenital and ocular toxoplasmosis
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Association between hospital onset of infection and outcomes in sepsis patients – A propensity score matched cohort study based on health claims data in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-12-01 Norman Rose, Melissa Spoden, Antje Freytag, Mathias Pletz, Tim Eckmanns, Lisa Wedekind, Josephine Storch, Peter Schlattmann, Christiane S. Hartog, Konrad Reinhart, Christian Günster, Carolin Fleischmann-Struzek
Background Hospital-acquired infections are a common source of sepsis. Hospital onset of sepsis was found to be associated with higher acute mortality and hospital costs, yet its impact on long-term patient-relevant outcomes and costs is unknown. Objective We aimed to assess the association between sepsis origin and acute and long-term outcomes based on a nationwide population-based cohort of sepsis
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Interdisciplinary studies on Coxiella burnetii: From molecular to cellular, to host, to one health research Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-11-29 Benjamin U. Bauer, Michael R. Knittler, Jennifer Andrack, Christian Berens, Amely Campe, Bahne Christiansen, Akinyemi M. Fasemore, Silke F. Fischer, Martin Ganter, Sophia Körner, Gustavo R. Makert, Svea Matthiesen, Katja Mertens-Scholz, Sven Rinkel, Martin Runge, Jan Schulze-Luehrmann, Sebastian Ulbert, Fenja Winter, Dimitrios Frangoulidis, Anja Lührmann
The Q-GAPS (Q fever GermAn interdisciplinary Program for reSearch) consortium was launched in 2017 as a German consortium of more than 20 scientists with exceptional expertise, competence, and substantial knowledge in the field of the Q fever pathogen Coxiella (C.) burnetii. C. burnetii exemplifies as a zoonotic pathogen the challenges of zoonotic disease control and prophylaxis in human, animal, and
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ListiWiki: A database for the foodborne pathogen Listeria monocytogenes Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-11-29 Christoph Elfmann, Bingyao Zhu, Jörg Stülke, Sven Halbedel
Listeria monocytogenes is a Gram positive foodborne pathogen that regularly causes outbreaks of systemic infectious diseases. The bacterium maintains a facultative intracellular lifestyle; it thrives under a variety of environmental conditions and is able to infect human host cells. L. monocytogenes is genetically tractable and therefore has become an attractive model system to study the mechanisms
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Effects of resveratrol on macrophages after phagocytosis of Candida glabrata Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-11-07 Zong-Han Chen, Meng Guan, Wei-Jia Zhao
Candida glabrata is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. C. glabrata-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying
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Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-10-31 Llanos Salar-Vidal, Catarina Chaves, Ileana T. Dianzo-Delgado, Patricio Favier, Salvador Giner-Almaraz, María José Gómez-Gómez, Guillermo Martín-Gutiérrez, Isabel Pereira, Ana Rodríguez-Fernández, Patricia Ruiz-Garbajosa, Carlos Salas-Venero, Jaime Esteban
Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms
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Polydatin alleviates mycoplasma pneumoniae-induced injury via inhibition of Caspase-1/GSDMD-dependent pyroptosis Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-09-26 Yiliu Chen, Yonghong Jiang, Xiuxiu Liu, Xiufeng Chen, Qiuyue Fan, Zhen Xiao
Mycoplasma pneumoniae (MP) is one of the main pathogens causing community acquired pneumonia (CAP) in children and adults. Previous pharmacological and clinical studies have shown that Polydatin (PD) exerts anti-inflammatory action by conferring protective benefit in MP pneumonia. However, the mechanism underlying the of PD on MP infection remains unclear. It was found that PD alleviated MP-induced
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Innate activation of human neutrophils and neutrophil-like cells by the pro-inflammatory bacterial metabolite ADP-heptose and Helicobacter pylori Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-06-28 Larissa Faass, Martina Hauke, Saskia C. Stein, Christine Josenhans
Lipopolysaccharide inner core heptose metabolites, including ADP-heptose, play a substantial role in the activation of cell-autonomous innate immune responses in eukaryotic cells, via the ALPK1-TIFA signaling pathway, as demonstrated for various pathogenic bacteria. The important role of LPS heptose metabolites during Helicobacter pylori infection of the human gastric niche has been demonstrated for
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Phenotypic and genotypic discrimination of Francisella tularensis ssp. holarctica clades Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-06-14 Kristin Köppen, Kerstin Rydzewski, Joerg Doellinger, Kerstin Myrtennäs, Mats Forsman, Sandra Appelt, Holger Scholz, Klaus Heuner
Francisella tularensis is the causative agent of tularemia, a zoonotic disease with a wide host range. F. tularensis ssp. holarctica (Fth) is of clinical relevance for European countries, including Germany. Whole genome sequencing methods, including canonical Single Nucleotide Polymorphism (canSNP) typing and whole genome SNP typing, have revealed that European Fth strains belong to a few monophyletic
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Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-06-03 Fansen Meng, Siying Zhu, Meiliang Gong, Hongjin Tao, Weihua Wang, Gangshi Wang
Polaprezinc (PZ) plays a role in the protection of gastric mucosa and inhibiting Helicobacter pylori (H. pylori) growth in vitro. The objective of this study was to determine the protective effects of PZ on human gastric epithelial cells (GES-1) against H. pylori-induced damage, while also examining heat shock protein 70 (HSP70) as a potential underlying factor in this protection. Our findings revealed
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Preclinical evaluation of 2-[18F]fluorodeoxysorbitol as a tracer for targeted imaging of Enterobacterales infection Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-05-16 Lisanne M. Braams, Jürgen W.A. Sijbesma, Hendrikus H. Boersma, Jan Maarten van Dijl, Philip H. Elsinga, Andor W.J.M. Glaudemans, Riemer H.J.A. Slart, Marleen van Oosten
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Longitudinal relationship between the gut microbiota variation and diversity and gut graft-versus-host disease (GVHD) following pediatric allogeneic hematopoietic cell transplantation (HCT) – Case series Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-04-25 Ashley N. Gray, Nicole H. Tobin, Theodore B. Moore, Fan Li, Grace M. Aldrovandi
Allogeneic Hematopoietic Cell Transplantation (HCT) offers children with life-threatening diseases a chance at survival. Complications from graft-versus-host disease (GVHD, Stages 0–4) represent a significant cause of morbidity and mortality which has been recently associated with gut dysbiosis the adult HCT population. Here, our objective was to conduct a prospective, longitudinal cohort study in
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Plasmodium falciparum rhoptry neck protein 4 has conserved regions mediating interactions with receptors on human erythrocytes and hepatocyte membrane Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-04-03 Fredy A. Pulido-Quevedo, Gabriela Arévalo-Pinzón, Jeimmy J. Castañeda-Ramírez, Adriana Barreto-Santamaría, Manuel E. Patarroyo, Manuel A. Patarroyo
Plasmodium falciparum-related malaria represents a serious worldwide public health problem due to its high mortality rates. P. falciparum expresses rhoptry neck protein 4 (PfRON4) in merozoite and sporozoite rhoptries, it participates in tight junction-TJ formation via the AMA-1/RON complex and is refractory to complete genetic deletion. Despite this, which PfRON4 key regions interact with host cells
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Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-03-28 Shasha Liu, Pengfei She, Zehao Li, Yimin Li, Linhui Li, Yifan Yang, Linying Zhou, Yong Wu
There has been an explosion in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) because of the indiscriminate use of antibiotics. In this study, we repurposed hexestrol (HXS) as an antibacterial agent to fight planktonic and biofilm-related MRSA infections. HXS is a nonsteroidal synthetic estrogen that targets estrogen receptors (ERα and ERβ) and has been used as a hormonal antineoplastic
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Mechanisms of folate metabolism-related substances affecting Staphylococcus aureus infection Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-02-16 Qiyuan Jin, Xiaolu Xie, Yaxuan Zhai, Haifang Zhang
Staphylococcus aureus (S. aureus) is one of the critical clinical pathogens which can cause multiple diseases ranging from skin infections to fatal sepsis. S. aureus is generally considered to be an extracellular pathogen. However, more and more evidence has shown that S. aureus can survive inside various cells. Folate plays an essential role in multiple life activities, including the conversion of
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Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR? Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-01-31 Neele J. Froböse, Ioana D. Olaru, Julia Sophie Schneider, Wenlan Zhang, Alexander Mellmann, Franziska Schuler, Tobias Grebe, Frieder Schaumburg
We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic Escherichia coli from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR enrichment in selective broth. In comparison to a reference method, sensitivities for stx1/2 and eae with and without
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Anti-inflammatory effect of chlorogenic acid in Klebsiella pneumoniae-induced pneumonia by inactivating the p38MAPK pathway Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-01-24 Yizhe Zhang, Chaoyin Zhu, Hongjun Zhao, Zhanyang Sun, Xiaodi Wang
Introduction Pneumonia is an inflammation-related respiratory infection and chlorogenic acid (CGA) possesses a wide variety of bioactive properties, such as anti-inflammation and anti-bacteria. Aim This study explored the anti-inflammatory mechanism of CGA in Klebsiella pneumoniae (Kp)-induced rats with severe pneumonia. Methods The pneumonia rat models were established by infection with Kp and treated
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Pretreatment with 3-methyladenine ameliorated Pseudomonas aeruginosa-induced acute pneumonia by inhibiting cell death of neutrophils in a mouse infection model Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-01-20 Lei Yue, Han Cao, Jialong Qi, Jin Yuan, Xin Wang, Yunfei Wang, Bin Shan, Huaxin Ke, Hua Li, Ning Luan, Cunbao Liu
Pseudomonas aeruginosa is one of the leading causes of nosocomial infections worldwide. Clinical isolates that are resistant to multiple antimicrobials make it intractable. The interactions between P. aeruginosa and host cell death have multiple effects on bacterial clearance and inflammation; however, the potential intervention effects remain to be defined. Herein, we demonstrated that intravenous
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Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2023-01-06 LiangZhe Liu, Carmen Oi Kwan Law, Qichang Nie, Hoa Quynh Pham, Haiying Ma, Liang Zhang, Pak Leung Ho, Terrence Chi-Kong Lau
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Nanopore 16S amplicon sequencing enables rapid detection of pathogen in knee periprosthetic joint infection Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-10-18 Hyuk-Soo Han, Du Hyun Ro, Jeehyeok Chung, Narae Kim, Jangsup Moon
Objectives We investigated whether nanopore 16S amplicon sequencing is capable of bacterial identification in patients with knee prosthetic joint infection (PJI), and we compared its efficacy with conventional culture studies. Methods In total, 36 patients who had clinical manifestation suspected of PJI were enrolled in this study. To begin, synovial fluids were aspirated from the affected knee using
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Chlamydia psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor expression Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-10-18 Zhangping He, Chuan Wang, Jianye Wang, Kang Zheng, Nan Ding, Maoying Yu, Weiwei Li, Yuanyuan Tang, Yumeng Li, Jian Xiao, Mingxing Liang, Yimou Wu
This study tested the hypothesis that Chlamydia psittaci (C. psittaci) survives and multiplies in human neutrophils by activating P2X7, a nonselective cationic channel receptor expressed constitutively on the surface of these cells. Findings illustrated that P2X7 receptor expression was enhanced in C. psittaci-infected neutrophils. C. psittaci was able to inhibite spontaneous apoptosis of neutrophils
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Mechanisms of promoting the differentiation and bone resorption function of osteoclasts by Staphylococcus aureus infection Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-09-27 Zelei Tong, Zhihao Chen, Ziyuan Li, Zonggang Xie, Haifang Zhang
Bone infection is a common and serious complication in the field of orthopedics, which frequently leads to excessive bone destruction and fracture nonunion. Staphylococcus aureus (S. aureus) infection affects bone cell function which, in turn, causes bone destruction. Bone is mainly regulated by osteoblasts and osteoclasts. Osteoclasts are the only cell type with bone resorptive function. Their over-activation
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MicroRNA-31 mediated by interferon regulatory factor 7 signaling facilitates control of Mycobacterium tuberculosis infection Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-09-27 Zhiyi Zhang, Qiongdan Mai, Lijia Yang, Yiwei Chen, Zixu Chen, Tao Lin, Shimin Tan, Zhiying Wu, Yongjie Cai, Taimei Cui, Beiyin Ouyang, Yi Yang, Lingchan Zeng, Zhenhuang Ge, Sien Zhang, Gucheng Zeng, Jiang Pi, Lingming Chen
Tuberculosis (TB) induced by Mycobacterium tuberculosis (M. tuberculosis) infection remains a global most deadly infectious disease. While development of more effective TB vaccines and therapeutics relies on identifications of true biomarkers designating an immune protection against M. tuberculosis infection, exact protective immune components against M. tuberculosis infection remain largely unidentified
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Machine learning on the road to unlocking microbiota’s potential for boosting immune checkpoint therapy Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-09-09 Szymon Wojciechowski, Monika Majchrzak-Górecka, Paweł Biernat, Krzysztof Odrzywołek, Łukasz Pruss, Konrad Zych, Jan Majta, Kaja Milanowska-Zabel
The intestinal microbiota is a complex and diverse ecological community that fulfills multiple functions and substantially impacts human health. Despite its plasticity, unfavorable conditions can cause perturbations leading to so-called dysbiosis, which have been connected to multiple diseases. Unfortunately, understanding the mechanisms underlying the crosstalk between those microorganisms and their
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Klebsiella pneumoniae activates the TGF-β signaling pathway to adhere to and invade intestinal epithelial cells via enhancing TLL1 expression Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-09-02 Jun Liu, Shiliang Zhang, Hao Pei, Fan Tu, Bin Liu, Jie Yan, Xuai Lin
Klebsiella pneumoniae is a gram-negative bacterium that can cause many diseases in hospitals and communities. Intestinal K. pneumoniae infections are relatively rare. Most K. pneumoniae infections begin with the colonization of the gastrointestinal system. In this study, clinically isolated K. pneumoniae strains were used to infect intestinal epithelial Caco-2 cells to study the possible intestinal
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Comprehensive investigation of antibiotic resistance gene content in cfiA-harboring Bacteroides fragilis isolates of human and animal origins by whole genome sequencing Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-08-08 Huiluo Cao, Melissa Chun-Jiao Liu, Man-Ki Tong, Shuo Jiang, Kin-Hung Chow, Kelvin Kai-Wang To, Cindy Wing-Sze Tse, Pak-Leung Ho
Introduction The emergence of multidrug resistance in Bacteroides fragilis, especially the phylogenetic lineage carrying the carbapenemase gene cfiA, represents an increasing threat to human health. However, knowledge on the diversity of the multidrug-resistant strains and the genetic elements carrying the antibiotic resistance genes (ARGs) remains limited. Aim The objective of the study was to describe
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Detection of OXA-181-producing Pseudomonas aeruginosa in Germany Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-07-08 Jennifer Schauer, Sören G. Gatermann, Jessica Eisfeld, Jörg Hans, Niels Pfennigwerth
Objectives To report the detection of the class D carbapenemase OXA-181 in an MDR clinical Pseudomonas aeruginosa isolate in Germany. Methods Carbapenemase detection was performed by using several phenotypic tests such as the modified Hodge test, a combined disc test with boronic acid, EDTA or cloxacillin, a lysate-based inhibition assays and by PCR for common and rare carbapenemase genes. Antibiotic
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Can Mycobacterium tuberculosis infection lead to cancer? Call for a paradigm shift in understanding TB and cancer Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-07-08 Asrar A. Malik, Javaid A. Sheikh, Nasreen Z. Ehtesham, Subhash Hira, Seyed E. Hasnain
Infections are known to cause tumours though more attributed to viruses. Strong epidemiological links suggest association between bacterial infections and cancers as exemplified by Helicobacter pylori and Salmonella spp. Infection with Mycobacterium tuberculosis (M. tb), the etiological agent of tuberculosis (TB), has been reported to predispose patients to lung cancers and possibly in other organs
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Regulatory interplay of RpoS and RssB controls motility and colonization in Vibrio cholerae Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-04-16 Martina Wölflingseder, Sarah Tutz, Vera H. Fengler, Stefan Schild, Joachim Reidl
Cholera is a life-threatening diarrheal disease caused by the human pathogenic bacterium Vibrio cholerae. Regulatory elements are essential for bacterial transition between the natural aquatic environment and the human host. One of them is the alternative sigma factor RpoS and its anti-sigma factor RssB. Regulation principles seem to be conserved among RpoS/RssB interaction modes between V. cholerae
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Treponema pallidum Tp0751 alters the expression of tight junction proteins by promoting bEnd3 cell apoptosis and IL-6 secretion Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-03-14 Simin Lu, Jianye Wang, Zhangping He, Siqin He, Kang Zheng, Man Xu, Shuai Yuan, Yimou Wu
Background Neurosyphilis is a serious complication caused by the invasion of the central nervous system by Treponema pallidum subsp. pallidum (T. pallidum). However, the molecular mechanism by which T. pallidum crosses the blood-brain barrier has not been fully elucidated. Objectives The primary purpose of this experimental design was to explore the effect of the T. pallidum adhesion protein Tp0751
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Characterization of the cagA-gene in Helicobacter pylori in Mongolia and detection of two EPIYA-A enriched CagA types Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-02-24 Oyunbaatar Altanbayar, Avarzed Amgalanbaatar, Chimeddorj Battogtokh, Narmandakh Bayarjargal, Dana Belick, Malte Kohns Vasconcelos, Colin R. Mackenzie, Klaus Pfeffer, Birgit Henrich
Helicobacter pylori infection is strongly associated with gastritis, gastroduodenal ulcer disease and gastric carcinoma. The virulence of H. pylori strains increases with the presence of the pathogenicity island PAI, which encodes a Type 4 Secretion System and the oncoprotein CagA. Two major CagA types can be distinguished by differences in the repetitive EPIYA region in the C-terminal sequence; the
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Global epidemiology of antimicrobial resistance in commensal Neisseria species: A systematic review Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-02-22 Thibaut Vanbaelen, Christophe Van Dijck, Jolein Laumen, Natalia Gonzalez, Irith De Baetselier, Sheeba S. Manoharan-Basil, Tessa De Block, Chris Kenyon
Background Commensal Neisseria species (spp). represent an important reservoir of antimicrobial resistance genes for pathogenic Neisseria spp. In this systematic review, we aimed to assess the antimicrobial susceptibility of commensal Neisseria spp. and how this has evolved over time. We also aimed to assess if commensal Neisseria spp. showed intrinsic resistance to four antimicrobials - penicillin
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Rectal swabs are a reliable method of assessing the colonic microbiome Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-01-24 Greg Turner, Michael O’Grady, Daniel Hudson, Xochitl Morgan, Frank Frizelle, Rachel Purcell
Background Advances in genome sequencing have enabled detailed microbiome analysis; however, the ideal specimen type for sequencing is yet to be determined. Rectal swabs may offer a rapid and convenient modality for colonic microbiome analysis. The aim of this study is to evaluate the use of rectal swabs compared to faecal specimens. Methods and results Twenty health professionals participated in this
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Virulent Staphylococcus aureus colonizes pediatric nares by resisting killing of human antimicrobial peptides Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-01-24 Ziyu Yang, Bijun Qiu, Danhong Cheng, Na Zhao, Yao Liu, Min Li, Qian Liu
Background The nasal carriage of Staphylococcus aureus introduces risks for subsequent infections, the rate of which is particularly high in children. The colonization mechanisms of S. aureus are not fully understood. Methods The epidemiological characteristics of nasal colonizing strains from pediatric patients undergoing liver transplantation and healthy pre-school children were analyzed first. Phenotypes
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Evolution of fluoroquinolone-resistant Escherichia coli in the gut after ciprofloxacin treatment Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2022-01-04 V. de Lastours, I. El Meouche, F. Chau, J. Beghain, D. Chevret, A. Aubert-Frambourg, O. Clermont, G. Royer, O. Bouvet, E. Denamur, B. Fantin
Background Three healthy volunteers carried similar quinolone-resistant E. coli (QREC) (pulsed field gel electrophoresis profiles) in their gut before and after 14 days ciprofloxacin treatment. Given the intensity of the selective pressure and the mutagenic properties of quinolones, we determined whether these strains had evolved at the phenotypic and/or genomic levels. Material and methods Commensal
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The changing molecular epidemiology of Enterococcus faecium harbouring the van operon at a teaching hospital in Western Australia: A fifteen-year retrospective study Int. J. Med. Microbiol. (IF 4.1) Pub Date : 2021-12-14 Terence Lee, Stanley Pang, Denise A. Daley, Julie C. Pearson, Sam Abraham, Geoffrey W. Coombs
Introduction Enterococcus faecium is an opportunistic pathogen that has become one of the leading causes of hospital acquired infection that are resistant to multiple critically important antimicrobials. Aim The objective of the study was to describe the molecular characteristics and relationship between major strains of E. faecium harbouring the van operon and to determine if the strains had increasing