样式: 排序: IF: - GO 导出 标记为已读
-
Paraflavitalea pollutisoli sp. nov., Pollutibacter soli gen. nov. sp. nov., Polluticoccus soli gen. nov. sp. nov., and Terrimonas pollutisoli sp. nov., four new members of the family Chitinophagaceae from polluted soil Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-03-12 Ze-Shen Liu, Xiao-Kang Wang, Ke-Huan Wang, Mei-Ling Yang, De-Feng Li, Shuang-Jiang Liu
A taxonomic investigation was conducted on four bacterial strains isolated from soil contaminated with polycyclic aromatic hydrocarbons and heavy metals. Phylogenetic analysis revealed that these strains belonged to the family . Examination of the 16S rRNA genes indicated that their sequence identities were below 97.6 % compared to any known and validly nominated bacterial species. The genomes of the
-
Stomatohabitans albus gen. nov., sp. nov., an oral living facultative anaerobic actinobacteria isolated form Steller sea lion, and proposal of Stomatohabitantaceae fam. nov. and Stomatohabitantales ord. nov Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-03-07 Zi-Wen Yang, Wen-Li Liu, Peng Zhang, Dan-Yuan Guo, Hong-Chuan Wang, Jia-Ling Li, Pan-Deng Wang, Gui-Xin Dong, Guo-Xing Nie, Wen-Jun Li
Two novel actinobacteria, designated as SYSU M7M538 and SYSU M7M531, were isolated from oral of in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol
-
Phylogenomic analysis of metagenome-assembled genomes indicates new taxa in the order Spirochaetales and proposal of Thalassospirochaeta sargassi gen. nov. sp. nov. from seaweeds Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-03-02 Kohli Pragya, Pannikurungottu Sreya, Lakshmanan Vighnesh, Dhurka Mahima, Mallick Sushmita, Chintalapati Sasikala, Chintalapati Venkata Ramana
Six metagenome-assembled genomes (JB008, JB007, JB015, JB003, JB004, and JB002) belonging to the order were generated from seaweed samples collected from the Gulf of Mannar, India. The binned genomes JB008 and JB007 shared highest 16S rRNA gene identity of 94.9 % and 92.2–93.4 %, respectively with uncultivated family members, and < 90 % identity with JC444. While, the bin JB015 showed 99.1 % identity
-
Halomontanus rarus gen. nov., sp. nov., a novel halophilic archaeon of the family Natrialbaceae from salt lakes on the Qinghai-Xizang Plateau Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-25 Ya-Ling Mao, Bei-Bei Wang, Xue-Meng Yin, Jing Hou, Heng-Lin Cui
Two halophilic archaeal strains TS33 and KZCA124 were isolated from two distant salt lakes on the Qinghai-Xizang Plateau, respectively. Culture-independent analysis indicated that these two strains were original inhabitants but low abundant taxa in respective salt lakes. Strains TS33 and KZCA124 were able to grow at 20–60 °C (optimum were 42 and 35 °C, respectively), with 0.9–4.8 M NaCl (optimum were
-
Quis custodiet ipsos custodes? A call for community participation in the governance of the SeqCode Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-24 Iain C. Sutcliffe, Luis M. Rodriguez-R, Stephanus N. Venter, William B. Whitman
Codes of nomenclature that provide well-regulated and stable frameworks for the naming of taxa are a fundamental underpinning of biological research. These Codes themselves require systems that govern their administration, interpretation and emendment. Here we review the provisions that have been made for the governance of the recently introduced Code of Nomenclature of Prokaryotes Described from Sequence
-
Phylogenomic and molecular markers based studies on clarifying the evolutionary relationships among Peptoniphilus species. Identification of several Genus-Level clades of Peptoniphilus species and transfer of some Peptoniphilus species to the genus Aedoeadaptatus Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-24 Megha Malhotra, Sarah Bello, Radhey S. Gupta
To clarify the evolutionary relationships among species, whose members show association with increased risk for prostate cancer, detailed phylogenomic and comparative analyses were conducted on their genome sequences. In phylogenetic trees based on core genome proteins and 16S rRNA gene sequences, species formed eight distinct clades, with and species branching between them. The observed clades designated
-
Description of a new genus of the Pectobacteriaceae family isolated from water in coastal brackish wetlands of the French Camargue region, Prodigiosinella gen. nov., including the new species Prodigiosinella aquatilis sp. nov Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-21 Nicole Hugouvieux-Cotte-Pattat, Jean-Pierre Flandrois, Jérôme Briolay, Sylvie Reverchon, Céline Brochier-Armanet
The family comprises plant pathogens able to provoke diverse diseases, including plant maceration due to the production of pectinases disrupting the plant cell wall. To better understand their diversity, a survey of pectinolytic bacteria was performed in brackish lakes of the French region La Camargue near the Mediterranean Sea. The genome of six atypical isolates was sequenced; their size is around
-
-
Description of the first cultured representative of “Candidatus Synoicihabitans” genus, isolated from deep-sea sediment of South China Sea Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-05 Tariq Ahmad, Sidra Erum Ishaq, Lewen Liang, Jialin Hou, Ruize Xie, Yijing Dong, Tiantian Yu, Fengping Wang
In this study we describe the first cultured representative of Synoicihabitans genus, a novel strain designated as LMO-M01, isolated from deep-sea sediment of South China Sea. This bacterium is a facultative aerobe, Gram-negative, non-motile, and has a globular-shaped morphology, with light greenish, small, and circular colonies. Analysis of the 16S rRNA gene sequences of strain LMO-M01 showed less
-
Multiphasic investigations imply transfer of orange-/red-pigmented strains of the bean pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens to a new species as C. aurantiacum sp. nov., elevation of the poinsettia pathogen C. flaccumfaciens pv. poinsettiae to the species level as C. Poinsettiae sp. nov., and synonymy of C. albidum with C. citreum Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-02-02 Ebrahim Osdaghi, S. Mohsen Taghavi, Mozhde Hamidizade, Mehdi Kariminejhad, Amal Fazliarab, Habibeh Hajian Maleki, Steve Baeyen, Geraldine Taghouti, Marie-Agnes Jacques, Johan Van Vaerenbergh, Perrine Portier
(), a plant-pathogenic coryneform species includes five pathovars with valid names and a number of proposed – but unvalidated – new members. In this study, phenotypic features and DNA similarity indexes were investigated among all members. Results showed that the pv. strains causing bacterial canker of in the USA as well as the orange-/red-pigmented strains of pv. pathogenic on dry beans in Iran are
-
First single-strain enrichments of Electrothrix cable bacteria, description of E. aestuarii sp. nov. and E. rattekaaiensis sp. nov., and proposal of a cable bacteria taxonomy following the rules of the SeqCode Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-01-22 Lea E. Plum-Jensen, Andreas Schramm, Ian P.G. Marshall
Cable bacteria are electrically conductive, filamentous Desulfobulbaceae, which are morphologically, functionally, and phylogenetically distinct from the other members of this family. Cable bacteria have not been obtained in pure culture and were therefore previously described as candidate genera, Candidatus Electrothrix and Ca. Electronema; a representative of the latter is available as single-strain
-
Halomonas rhizosphaerae sp. nov. and Halomonas kalidii sp. nov., two novel moderate halophilic phenolic acid-degrading species isolated from saline soil Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2024-01-19 Lian Xu, Hua-Mei Wei, Ye-Nan Sun, Qi Wu, Xiao-Yan Gao, Biao Shen, Ji-Quan Sun
Four vanillic acid-degrading bacterial strains, named LR5S13T, LR5S20, and M4R5S39T and LN1S58, were isolated from Kalidium cuspidatum rhizosphere and bulk soils, respectively. Phylogenetic analysis based on 16S rRNA gene as well as core genome revealed that LR5S13T and LR5S20 clustered closely with each other and with Halomonas ventosae Al12T, and that the two strains shared the highest similarities
-
In the footsteps of Heinz Schlesner and Peter Hirsch: Exploring the untapped diversity of the phylum Planctomycetota in isolates from the 1980s to the early 2000s Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-12-12 Nicolai Kallscheuer, Carmen E. Wurzbacher, Ruth A. Schmitz, Christian Jogler
Recent sampling and strain isolation campaigns have accelerated research on the bacterial phylum Planctomycetota. The contribution of more than 100 novel isolates to the open collection of currently 123 described planctomycetal species in the last decade benefited greatly from pioneering work conducted in the second half of the last century. One of those pioneers was Heinz Schlesner, who investigated
-
Andean soil-derived lignocellulolytic bacterial consortium as a source of novel taxa and putative plastic-active enzymes Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-12-10 Laura Díaz-García, Maria Chuvochina, Golo Feuerriegel, Boyke Bunk, Cathrin Spröer, Wolfgang R. Streit, Luis M. Rodriguez-R, Jörg Overmann, Diego Javier Jiménez
An easy and straightforward way to engineer microbial environmental communities is by setting up liquid enrichment cultures containing a specific substrate as the sole source of carbon. Here, we analyzed twenty single-contig high-quality metagenome-assembled genomes (MAGs) retrieved from a microbial consortium (T6) that was selected by the dilution-to-stimulation approach using Andean soil as inoculum
-
-
Corrigendum to “Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes” [Syst. Appl. Microbiol. 46(6) (2023) 126471] Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-11-30 Dimitry Y. Sorokin, Alexander G. Elcheninov, Alexander Y. Merkel, Nicole J. Bale, Jaap Sininghe-Damste, Ilya V. Kublanov
Abstract not available
-
Multilocus sequence and phenotypic analysis of Pectobacterium and Dickeya type strains for identification of soft rot Pectobacteriaceae from symptomatic potato stems and tubers in Pennsylvania Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-11-26 Amanda M. Mainello-Land, Shaheen Bibi, Beth Gugino, Carolee T. Bull
Outbreaks of potato blackleg and soft rot caused by Pectobacterium species and more recently Dickeya species across the U.S. mid-Atlantic region have caused yield loss due to poor emergence as well as losses from stem and tuber rot. To develop management strategies for soft rot diseases, we must first identify which members of the soft rot Pectobacteriaceae are present in regional potato plantings
-
A novel method for the whole-cell detection of environmental microorganisms using hemin and tyramide signal amplification (Hemin-TSA) with a desired fluorescent dye Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-11-11 Kampachiro Urasaki, Yu-You Li, Kengo Kubota
A method called hemin-tyramide signal amplification (Hemin-TSA) was developed for visualization of environmental microorganisms using hemin and tyramide signal amplification. In Hemin-TSA, hemin, which has peroxidase activity, is bound to microbial cells, and a desired fluorescent dye is deposited on the microbial cells by a hemin-catalyzed TSA reaction. The protocol was initially optimized in terms
-
Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-10-07 Dimitry Y. Sorokin, Alexander G. Elcheninov, Alexander Y. Merkel, Nicole J. Bale, Jaap Sininghe-Damste, Ilya V. Kublanov
Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to phylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae. The main phenotypic property of the novel isolates is the
-
Mycoplasma bradburyae sp. nov. isolated from the trachea of sea birds Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-10-07 Ana S. Ramírez, José B. Poveda, Remco Dijkman, Carlos Poveda, Alejandro Suárez-Pérez, Rubén S. Rosales, Anneke Feberwee, Michael P. Szostak, Lorenzo Ressel, Tomeu Viver, Pascual Calabuig, Salvatore Catania, Federica Gobbo, Dorina Timofte, Joachim Spergser
In the search for mollicutes in wild birds, six Mycoplasma strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (Larus michahellis) and a Cory’s shearwater (Calonectris borealis) from Spain, one from a South African Kelp gull (Larus dominicanus), and one from an Italian Black-headed gull (Chroicocephalus ridibundus)
-
-
Hidden from plain sight: Novel Simkaniaceae and Rhabdochlamydiaceae diversity emerging from screening genomic and metagenomic data Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-09-23 Helen R. Davison, Gregory D.D. Hurst
-
Two novel symbiovars of Bradyrhizobium yuanmingense, americaense and caribense, the symbiovar tropici of Bradyrhizobium pachyrhizi and the symbiovar cajani of Bradyrhizobium cajani are microsymbionts of the legume Cajanus cajan in Dominican Republic Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-09-04 José David Flores-Félix, Fernando Sánchez-Juanes, Juan Araujo, César Antonio Díaz-Alcántara, Encarna Velázquez, Fernando González-Andrés
Cajanus cajan L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop. Here we identified through MALDI-TOF MS several rhizobial strains nodulating C. cajan in two Dominican locations as Bradyrhizobium yuanmingense. The phylogenetic analysis of recA and glnII housekeeping genes showed that these strains belong to a wide cluster together with the type strain
-
Corrigendum to “Expanding the rumen Prevotella collection: the description of Prevotella communis, sp. nov. of ovine origin” [Syst. Appl. Microbiol. 46(4) (2023) 126437] Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-09-03 Eva Grabner, Eva Stare, Lijana Fanedl, Maša Zorec, Dakota S. Jones, Christopher D. Johnston, Gorazd Avguštin, Tomaž Accetto
Abstract not available
-
Bradyrhizobium xenonodulans sp. nov. isolated from nodules of Australian Acacia species invasive to South Africa Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-08-18 Ricu Claassens, Stephanus N. Venter, Chrizelle W. Beukes, Tomasz Stępkowski, Wai Y. Chan, Emma T. Steenkamp
A genealogical concordance approach was used to delineate strains isolated from Acacia dealbata and Acacia mearnsii root nodules in South Africa. These isolates form part of Bradyrhizobium based on 16S rRNA sequence similarity. Phylogenetic analysis of six housekeeping genes (atpD, dnaK, glnII, gyrB, recA and rpoB) confirmed that these isolates represent a novel species, while pairwise average nucleotide
-
Sedimenticola hydrogenitrophicus sp. nov. a chemolithoautotrophic bacterium isolated from a terrestrial mud volcano, and proposal of Sedimenticolaceae fam. nov. in the order Chromatiales Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-08-06 Galina Slobodkina, Alexander Merkel, Nataliya Ratnikova, Alexandra Kuchierskaya, Alexander Slobodkin
Chemolithoautotrophic microorganisms can play a significant role in the biogeochemical cycling of elements in deep-subsurface-associated environments. A novel facultatively anaerobic lithoautotrophic bacteria (strains SB48T and SN1189) were isolated from terrestrial mud volcanoes (Krasnodar Krai, Russia). Cells of the strains were straight motile rods. Growth was observed at temperatures up to 35 °C
-
Corrigendum to “Sphingomonas pokkalii sp. nov., a novel plant associated rhizobacterium isolated from a saline tolerant pokkali rice and its draft genome analysis” [Syst. Appl. Microbiol. 42(3) (2019) 334–342] Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-08-04 Rahul R. Menon, Sunitha Kumari, Pravin Kumar, Ashish Verma, Srinivasan Krishnamurthi, N. Rameshkumar
Abstract not available
-
Brytella acorum gen. nov., sp. nov., a novel acetic acid bacterium from sour beverages Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-07-04 Atena Sadat Sombolestani, Dries Bongaerts, Eliza Depoorter, Ilse Cleenwerck, Anneleen D. Wieme, Scott J. Britton, Stefan Weckx, Luc De Vuyst, Peter Vandamme
Polyphasic taxonomic and comparative genomic analyses revealed that a series of lambic beer isolates including strain LMG 32668T and the kombucha isolate LMG 32879 represent a novel species among the acetic acid bacteria, with Acidomonas methanolica as the nearest phylogenomic neighbor with a valid name. Overall genomic relatedness indices and phylogenomic and physiological analyses revealed that this
-
Describing difficult-to-culture bacteria: Taking a shortcut or investing time to discover something new? Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-06-30 Svetlana N. Dedysh
Despite the growing interest in isolating representatives of poorly studied and as-yet-uncultivated bacterial phylogenetic groups, these microorganisms remain difficult objects for taxonomic studies. The time required for describing one of these fastidious bacteria is commonly measured in several years. What is even more problematic, many routine laboratory tests, which were originally developed for
-
Fontisphaera persica gen. nov., sp. nov., a thermophilic hydrolytic bacterium from a hot spring of Baikal lake region, and proposal of Fontisphaeraceae fam. nov., and Limisphaeraceae fam. nov. within the Limisphaerales ord. nov. (Verrucomicrobiota) Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-05-26 Olga A. Podosokorskaya, Alexander G. Elcheninov, Andrei A. Novikov, Alexander Y. Merkel, Ilya V. Kublanov
A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 T, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30–57 °C and within a pH range of 5.1–8.4 with the optimum at 50 °C and pH 6.8–7.1. Strain
-
Expanding the rumen Prevotella collection: The description of Prevotella communis, sp. nov. of ovine origin Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-05-30 Eva Grabner, Eva Stare, Lijana Fanedl, Maša Zorec, Dakota S. Jones, Christopher D. Johnston, Gorazd Avguštin, Tomaž Accetto
27 strains representing eight new Prevotella species were isolated from rumen of a single sheep in eight weeks interval. One of the putative species encompassing the highest number of isolated strains which also exhibited some genetic variability in preliminary data, was then selected for description of a novel species. We examined six strains in genomic and phenotypic detail, two of which may actually
-
Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-05-15 Mohamed Hnini, Imane El Attar, Kaoutar Taha, Jamal Aurag
In the present study, we analyzed the genetic diversity, phylogenetic relationships, stress tolerance, phytobeneficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of Vachellia tortilis subsp. raddiana grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, 16S rDNA gene sequencing of 15
-
Microbial community on industrial salty bovine hides: From the slaughterhouse to the salting Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-05-18 Francisco Nadal-Molero, Alicia Campos-Lopez, Juan Tur-Moya, Ana-Belen Martin-Cuadrado
The leather-making industry is an age-old industry and desiccation with salt has been one of the most used methodologies for obtaining valuable skins. However, halophiles may proliferate and affect the integrity of the hide-collagen structure, as well as leading to undesirable red colorations or less-frequent purple stains. To understand the basis of these industrial hide contaminations, the microbial
-
Novel symbiovars ingae, lysilomae and lysilomaefficiens in bradyrhizobia from tree-legume nodules Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-05-11 Diana Hernández-Oaxaca, Karen Claro, Marco A. Rogel, Mónica Rosenblueth, Julio Martinez-Romero, Esperanza Martinez-Romero
-
Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov. Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-29 Irena Todorović, Danis Abrouk, Martina Kyselková, Céline Lavire, Marjolaine Rey, Vera Raičević, Jelena Jovičić-Petrović, Yvan Moënne-Loccoz, Daniel Muller
Pseudomonas strains IT-194P, IT-215P, IT-P366T and IT-P374T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366T and
-
Identification, classification, and functional characterization of novel sponge-associated acidimicrobiial species Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-28 Viet Hung Nguyen, Bernd Wemheuer, Weizhi Song, Holly Bennett, Nicole Webster, Torsten Thomas
Sponges are known to harbour an exceptional diversity of uncultured microorganisms, including members of the phylum Actinobacteriota. While members of the actinobacteriotal class Actinomycetia have been studied intensively due to their potential for secondary metabolite production, the sister class of Acidimicrobiia is often more abundant in sponges. However, the taxonomy, functions, and ecological
-
Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-28 Victoria Werum, Matthias Ehrmann
The study provides a taxonomic characterization of three bacterial strains isolated from high-oxygen modified-atmosphere packaged beef from Germany. The strains of the novel species shared identical 16S rRNA gene sequence to the closely related type strain of Dellaglioa algida. However, the in-silico DNA-DNA hybridization (DDH) values indicate that they belong to a different genomic species. The in
-
How diverse a genus can be: An integrated multi-layered analysis into Desmonostoc (Nostocaceae, Cyanobacteriota) Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-22 Allan Victor M. Almeida, Marcelo Gomes Marçal Vieira Vaz, Naira Valle de Castro, Diego Bonaldo Genuário, Jean Coutinho Oder, Pedro Augusto Marazzo de Souza, Sandy Bastos Martins, Mariana Machado, Adriano Nunes-Nesi, Wagner L. Araújo
Cyanobacteria (Phylum Cyanobacteriota) are Gram-negative bacteria capable of performing oxygenic photosynthesis. Although the taxonomic classification of cyanobacteria was for a long time based primarily on morphological characters, the application of other techniques (e.g. molecular phylogeny), especially in recent decades, has contributed to a better resolution of cyanobacteria systematics, leading
-
Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-26 Dana Nováková, Vendula Koublová, Karel Sedlář, Eva Staňková, Stanislava Králová, Pavel Švec, Meina Neumann-Schaal, Jacqueline Wolf, Sylva Koudelková, Miloš Barták, Ivo Sedláček
A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed
-
Phylogenomics reveals insights into the functional evolution of the genus Agrobacterium and enables the description of Agrobacterium divergens sp. nov Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-01 Henry D. Naranjo, Liesbeth Lebbe, Margo Cnockaert, Florent Lassalle, Chin Chin Too, Anne Willems
The genus Agrobacterium was initially described as mainly phytopathogenic strains. Nowadays, the genus includes phytopathogenic and non-phytopathogenic bacteria that are distinctive among the Rhizobiaceae family. Recently we have isolated two closely related strains, LMG 31531T and LMG 31532, from soil and plant roots, respectively. Both strains differ from previously reported species based on the
-
Ancylobacter mangrovi sp. nov., a novel endophytic bacterium isolated form mangrove plant Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-04-01 Fei-Na Li, Zhou-Qing Zheng, Ming-Sheng Chen, Xiao-Hui Chen, Li Tuo
Two novel strains GSK1Z-4-2T and MQZ15Z-1 were isolated from branches of mangrove plants collected from Guangxi Zhuang Autonomous Region, China. Both strains were Gram-negative, aerobic, non-flagellated and non-spore-forming bacteria. The comparison of 16S rRNA gene sequences initially indicated that the two strains were assigned to the genus Ancylobacter with sharing the highest similarity to Ancylobacter
-
Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov. Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-03-15 Tomeu Viver, Roth E. Conrad, Marianna Lucio, Mourad Harir, Mercedes Urdiain, Juan F. Gago, Ana Suárez-Suárez, Esteban Bustos-Caparros, Rodrigo Sanchez-Martinez, Eva Mayol, Federico Fassetta, Jinfeng Pang, Ionuț Mădălin Gridan, Stephanus Venter, Fernando Santos, Bonnie Baxter, María E. Llames, Adorján Cristea, Horia L. Banciu, Brian P. Hedlund, Ramon Rossello-Mora
Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new Salinibacter species. For these species we propose
-
Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-03-11 Leandro S. Velez, Flávia F. Aburjaile, Antonio R.G. Farias, Ana D.B. Baia, Willams J. Oliveira, Adriano M.F. Silva, Ana M. Benko-Iseppon, Vasco Azevedo, Bertram Brenig, Jong H. Ham, Elineide B. Souza, Marco A.S. Gama
Two putative novel Burkholderia cenocepacia lineages found in the semi-arid region of north-east Brazil causing onion sour skin were studied using genomic approaches to determine their taxonomic position. Four strains belonging to one novel lineage (CCRMBC16, CCRMBC33, CCRMBC74, and CCRMBC171) and one strain (CCRMBC51) belonging to another novel lineage had their whole genome sequenced to carry out
-
Govania unica gen. nov., sp. nov., a rare biosphere bacterium that represents a novel family in the class Alphaproteobacteria Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-03-05 Peter Vandamme, Charlotte Peeters, Amanda Hettiarachchi, Margo Cnockaert, Aurélien Carlier
Strain LMG 31809 T was isolated from a top soil sample of a temperate, mixed deciduous forest in Belgium. Comparison of its 16S rRNA gene sequence with that of type strains of bacteria with validly published names positioned it in the class Alphaproteobacteria and highlighted a major evolutionary divergence from its near neighbor species which represented species of the orders Emcibacterales and Sphingomonadales
-
Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-02-26 Veronika V. Koziaeva, Dimitry Y. Sorokin, Tatiana V. Kolganova, Denis S. Grouzdev
A spiral-shaped, highly motile bacterium was isolated from freshwater sulfidic sediment. Strain J10T is a facultative autotroph utilizing sulfide, thiosulfate, and sulfur as the electron donors in microoxic conditions. Despite high 16S rRNA gene sequence sequence identity to Magnetospirillum gryphiswaldense MSR-1 T (99.6 %), digital DNA-DNA hybridisation homology and average nucleotide identity between
-
Halonatronomonas betaini gen. nov., sp. nov., a haloalkaliphilic isolate from soda lake capable of betaine degradation and proposal of Halarsenatibacteraceae fam. nov. and Halothermotrichaceae fam. nov. within the order Halanaerobiales Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-02-26 Yulia V. Boltyanskaya, Vadim V. Kevbrin, Denis S. Grouzdev, Ekaterina N. Detkova, Veronika V. Koziaeva, Andrey A. Novikov, Tatjana N. Zhilina
A search for the organisms responsible for anaerobic betaine degradation in soda lakes resulted in isolation of a novel bacterial strain, designated Z-7014T. The cells were Gram-stain-negative, non-endospore-forming rods. Growth occurred at 8–52 °C (optimum 40–45 °C), pH 7.1–10.1 (optimum pH 8.1–8.8) and 1.0–3.5 M Na+ (optimum 1.8 M), i.e. it can be regarded as a haloalkaliphile. The strain utilized
-
Dominance of mixed ether/ester, intact polar membrane lipids in five species of the order Rubrobacterales: Another group of bacteria not obeying the “lipid divide” Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-02-24 Jaap S. Sinninghe Damsté, W. Irene C. Rijpstra, Katharina J. Huber, Luciana Albuquerque, Conceição Egas, Nicole J. Bale
The composition of the core lipids and intact polar lipids (IPLs) of five Rubrobacter species was examined. Methylated (ω-4) fatty acids (FAs) characterized the core lipids of Rubrobacter radiotolerans, R. xylanophilus and R. bracarensis. In contrast, R. calidifluminis and R. naiadicus lacked ω-4 methyl FAs but instead contained abundant (i.e., 34–41 % of the core lipids) ω-cyclohexyl FAs not reported
-
Stutzerimonas decontaminans sp. nov. isolated from marine polluted sediments Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-23 Magdalena Mulet, Margarita Gomila, Jorge Lalucat, Rafael Bosch, Ramon Rossello-Mora, Elena García-Valdés
Strains 19SMN4T and ST27MN3 were isolated from marine sediments after enrichment with 2-methylnaphthalene and were classified as Pseudomonas stutzeri genomovar 4. Four other strains, BG 2, HT20, HT24, and A7, were isolated from sulphide-oxidizing bioreactors or activated sludge affiliated with the same clade in the 16S rRNA phylogenetic tree. P. stutzeri has been recently reclassified as a new genus
-
Functional characterization and taxonomic classification of novel gammaproteobacterial diversity in sponges Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-25 Viet Hung Nguyen, Bernd Wemheuer, Weizhi Song, Holly Bennett, Giorgia Palladino, Ilia Burgsdorf, Sofia Sizikov, Laura Steindler, Nicole S. Webster, Torsten Thomas
Sponges harbour exceptionally diverse microbial communities, whose members are largely uncultured. The class Gammaproteobacteria often dominates the microbial communities of various sponge species, but most of its diversity remains functional and taxonomically uncharacterised. Here we reconstructed and characterised 32 metagenome-assembled genomes (MAGs) derived from three sponge species. These MAGs
-
Agrobacterium cucumeris sp. nov. isolated from crazy roots on cucumber (Cucumis sativus) Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-25 Michał Warabieda, Nemanja Kuzmanović, Paweł Trzciński, Joanna Puławska
Three plant rhizogenic strains O132T, O115 and O34 isolated from Cucumis sp. L. were assessed for taxonomic affiliation by using polyphasic taxonomic methods. Based on the results of the sequence analysis of the 16S rRNA and multilocus sequence analysis (MLSA) of the three housekeeping genes atpD, recA and rpoB, all the strains were clustered within the genus Agrobacterium where they form a novel branch
-
Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-25 Tatjana N. Zhilina, Dimitry Y. Sorokin, Stepan V. Toshchakov, Ilya V. Kublanov, Daria G. Zavarzina
Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum OmegaT is an obligate aerobic proteolytic microorganism from a cyanobacterial
-
Methylomonas rapida sp. nov., a novel species of fast-growing, carotenoid-producing obligate methanotrophs with high biotechnological potential Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-24 Ekaterina N. Tikhonova, Ruslan Z. Suleimanov, Kirill K. Miroshnikov, Igor Y. Oshkin, Svetlana E. Belova, Olga V. Danilova, Aleksandr A. Ashikhmin, Aleksey A. Konopkin, Sergey Y. But, Valentina N. Khmelenina, Nikolai V. Pimenov, Svetlana N. Dedysh
The genus Methylomonas accommodates strictly aerobic, obligate methanotrophs, with their sole carbon and energy sources restricted to methane and methanol. These bacteria inhabit oxic-anoxic interfaces of various freshwater habitats and have attracted considerable attention as potential producers of a single-cell protein. Here, we characterize two fast-growing representatives of this genus, strains
-
Description of Dryocola gen. nov. and two novel species, Dryocola boscaweniae sp. nov. and Dryocola clanedunensis sp. nov. isolated from the rhizosphere of native British oaks Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-18 Daniel Maddock, Carrie Brady, Sandra Denman, Dawn Arnold
While investigating the role of the rhizosphere in the development of Acute Oak Decline, bacterial strains belonging to the family Enterobacteriaceae were isolated from rhizosphere soil following enrichment for the Enterobacterales. Partial sequencing of several housekeeping genes showed that these strains could not be assigned to an existing genus. Overall, 16 strains were investigated using a polyphasic
-
Description and genomic characterization of Nocardioides bruguierae sp. nov., isolated from Bruguiera gymnorhiza Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2023-01-02 Xiaohui Chen, Zhouqing Zheng, Feina Li, Xiao Ma, Feng Chen, Mingsheng Chen, Li Tuo
Strains BSK12Z-3T and BSK12Z-4, two Gram-stain-positive, aerobic, non-spore-forming strains, were isolated from Shankou Mangrove Nature Reserve, Guangxi Zhuang Autonomous Region, China. The diagnostic diamino acid in the cell-wall peptidoglycan of strain BSK12Z-3T was LL-diaminopimelic acid and MK-8(H4) was the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylglycerol
-
Corynebacterium antarcticum sp. nov., Corynebacterium marambiense sp. nov., Corynebacterium meridianum sp. nov., and Corynebacterium pygosceleis sp. nov., isolated from Adélie penguins (Pygoscelis adeliae) Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-12-21 Pavel Švec, Hans-Jürgen Busse, Karel Sedlář, Jana Musilová, Stanislava Králová, Eva Staňková, Ondrej Šedo, Daniel Krsek, Vendula Koublová, Lucie Krištofová, Ivo Sedláček
A taxonomic study was conducted on 16 bacterial strains isolated from wild Adélie penguins (Pygoscelis adeliae) from Seymour (Marambio) Island and James Ross Island. An initial screening by repetitive sequence-based PCR fingerprinting divided the strains studied into four coherent groups. Phylogenetic analysis based on 16S rRNA gene sequences assigned all groups to the genus Corynebacterium and showed
-
Weizmannia acidilactici sp. nov., a lactic acid producing bacterium isolated from soils Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-12-14 Vasana Tolieng, Naoto Tanaka, Yuh Shiwa, Sitanan Thitiprasert, Pawina Kanchanasin, Wongsakorn Phongsopitanun, Auttaporn Booncharoen, Nuttha Thongchul, Somboon Tanasupawat
-
Taxonomic proposal for a deep branching bacterial phylogenetic lineage: transfer of the family Thermodesulfobiaceae to Thermodesulfobiales ord. nov., Thermodesulfobiia classis nov. and Thermodesulfobiota phyl. nov Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-12-02 Evgenii N. Frolov, Alexander V. Lebedinsky, Alexander G. Elcheninov, Ilya V. Kublanov
The family Thermodesulfobiaceae, comprising one genus Thermodesulfobium with two validly published species, is currently assigned to order Thermoanaerobacterales within the class Clostridia of the phylum Bacillota. At the same time, the very first 16S rRNA gene sequence-based phylogenetic studies of representatives of the genus pointed out great differences between Thermodesulfobium and other members
-
Genomic insight into Chryseobacterium turcicum sp. nov. and Chryseobacterium muglaense sp. nov. isolated from farmed rainbow trout in Turkey Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-11-13 Izzet Burcin Saticioglu, Hilal Ay, Soner Altun, Muhammed Duman
Four strains, designated as C-2, C-17T, C-39T and Ch-15, were isolated from farmed rainbow trout samples showing clinical signs during an investigation for a fish-health screening study. The pairwise 16S rRNA gene sequence analysis showed that strain C-17T shared the highest identity level of 98.1 % with the type strain of Chryseobacterium piscium LMG 23089T while strains C-2, C-39T and Ch-15 were
-
Novel species of Frankia, Frankia gtarii sp. nov. and Frankia tisai sp. nov., isolated from a root nodule of Alnus glutinosa Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-11-01 Imen Nouioui, Faten Ghodhbane-Gtari, Gabriele Pötter, Hans-Peter Klenk, Michael Goodfellow
The status of four Frankia strains isolated from a root nodule of Alnus glutinosa was established in a polyphasic study. Taxogenomics and phenotypic features show that the isolates belong to the genus Frankia. All four strains form extensively branched substrate mycelia, multilocular sporangia, vesicles, lack aerial hyphae, but contain meso-diaminopimelic acid as the diamino acid of the peptidoglycan
-
Description of a moderately acidotolerant and aerotolerant anaerobic bacterium Acidilutibacter cellobiosedens gen. nov., sp. nov. within the family Acidilutibacteraceae fam. nov., and proposal of Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov. Syst. Appl. Microbiol. (IF 3.4) Pub Date : 2022-11-01 Hui Fan, Jiang Li, Weidong Wu, Rui Chen, Min Yang, Yinggang Zhang, Lulu Cong, Lirong Dai, Yu Deng, Lei Cheng, Shichun Ma
A Gram-stain positive, moderately thermophilic, acidotolerant and aerotolerant anaerobic bacterium, designated JN-28 T, was isolated from the pit mud of Chinese strong-flavor liquor. Growth was observed at 25–50 °C and pH 5.5–8.0 in the presence of 0–25 g l−1 NaCl (optimally at 45 °C, pH 6.0, without NaCl). Strain JN-28 T was heterotrophic, requiring yeast extract for growth. The major cellular fatty