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Fungal microtubule organizing centers are evolutionarily unstable structures Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-03-13 Adam Grazzini, Ann M. Cavanaugh
For most Eukaryotic species the requirements of cilia formation dictate the structure of microtubule organizing centers (MTOCs). In this study we find that loss of cilia corresponds to loss of evolutionary stability for fungal MTOCs. We used iterative search algorithms to identify proteins homologous to those found in , and MTOCs, and calculated site-specific rates of change for those proteins that
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Utilization of plant-derived sugars and lipids are coupled during colonization of rhizoplane and rhizosphere by the fungus Metarhizium robertsii Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-03-12 Jin Dai, Xingyuan Tang, Congcong Wu, Shuxing Liu, Wubin Mi, Weiguo Fang
Plant-derived sugars and lipids are key nutritional sources for plant associated fungi. However, the relationship between utilization of host-derived sugars and lipids during development of the symbiotic association remains unknown. Here we show that the fungus also needs plant-derived lipids to develop symbiotic relationship with plants. The fatty acid binding proteins FABP1 and FABP2 are important
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Coordination of two regulators SscA and VosA in Aspergillus nidulans conidia Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-03-04 Ye-Eun Son, Hee-Soo Park
Airborne fungal spores are a major cause of fungal diseases in humans, animals, and plants as well as contamination of foods. Previous studies found a variety of regulators including VosA, VelB, WetA, and SscA for sporogenesis and the long-term viability in To gain a mechanistic understanding of the complex regulatory mechanisms in asexual spores, here, we focused on the relationship between VosA and
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The genus Acrophialophora: History, phylogeny, morphology, beneficial effects and pathogenicity Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-02-15 Zoha Daroodi, Parissa Taheri
The genus is a thermotolerant fungus, which is widely distributed in temperate and tropical zones. This fungus is classified in Ascomycota and belongs to the Chaetomiaceae family and the genera of , and are closely related to For this genus have been reported 28 species so far, which two species of and produce only sexual phase and other species produce asexual form. Therefore, producing both sexual
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The serine-threonine protein kinase Snf1 orchestrates the expression of plant cell wall-degrading enzymes and is required for full virulence of the maize pathogen Colletotrichum graminicola Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-02-15 Alan de Oliveira Silva, Bennet Rohan Fernando Devasahayam, Lala Aliyeva-Schnorr, Chirlei Glienke, Holger B. Deising
Colletotrichum graminicola, the causal agent of maize leaf anthracnose and stalk rot, differentiates a pressurized infection cell called an appressorium in order to invade the epidermal cell, and subsequently forms biotrophic and necrotrophic hyphae to colonize the host tissue. While the role of force in appressorial penetration is established (), the involvement of cell wall-degrading enzymes (CWDEs)
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Role of Pmk1, Mpk1, or Hog1 in the mitogen-activated protein kinase pathway of Aspergillus cristatus Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-02-01 Lulu Liu, Longyue Li, Fengyi Li, Wei Ma, Wei Guo, Xu Fang
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RHO-3 plays a significant role in hyphal extension rate, conidiation, and the integrity of the Spitzenkörper in Neurospora crassa Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-01-22 Martha M. Ornelas-Llamas, Luis L. Pérez-Mozqueda, Olga A. Callejas-Negrete, Ernestina Castro-Longoria
The Rho family of monomeric GTPases act as signaling proteins to establish and maintain cell polarity and other essential cellular processes. Rho3 is a GTPase of the Rho family that is exclusive of fungi that regulate cell polarity in yeast. However, studies have yet to explore its function in filamentous fungi. In this work, we investigated the role of RHO-3 in the model organism Neurospora crassa
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AwSclB regulates a network for Aspergillus westerdijkiae asexual sporulation and secondary metabolism independent of the fungal light control Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-01-20 Gang Wang, Yibing Liu, Yafan Hu, Jiaqi Pan, Zifan Wei, Bowen Tai, Bolei Yang, Erfeng Li, Fuguo Xing
As a prevalent pathogenic fungus, Aspergillus westerdijkiae poses a threat to both food safety and human health. The fungal growth, conidia production and ochratoxin A (OTA) in A. weterdijkiae are regulated by many factors especially transcription factors. In this study, a transcription factor AwSclB in A. westerdijkiae was identified and its function in asexual sporulation and OTA biosynthesis was
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Genomic, transcriptomic, and ecological diversity of Penicillium species in cheese rind microbiomes Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-01-11 Ruby Ye, Megan Biango-Daniels, Jacob L. Steenwyk, Antonis Rokas, Nicolas L. Louw, Robert Nardella, Benjamin E. Wolfe
Although Penicillium molds can have significant impacts on agricultural, industrial, and biomedical systems, the ecological roles of Penicillium species in many microbiomes are not well characterized. Here we utilized a collection of 35 Penicillium strains isolated from cheese rinds to broadly investigate the genomic potential for secondary metabolism in cheese-associated Penicillium species, the impact
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Methyl jasmonate differentially and tissue-specifically regulated the expression of arginine catabolism–related genes and proteins in Agaricus bisporus mushrooms during storage Fungal Genet. Biol. (IF 3.0) Pub Date : 2024-01-09 Hanyue Jiang, Huadong Wang, Xiuhong Wang, Yating Wang, Rui Song, Shuai Yuan, Zhenchuan Fan, Demei Meng
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Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-27 Perng-Kuang Chang
Aspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce
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Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-19 M. Cogliati, P.E. Chidebelu, M. Hitchcock, M. Chen, V. Rickerts, S. Ackermann, M. Desnos Ollivier, J. Inácio, U. Nawrot, M. Florek, K.J. Kwon-Chung, D.-H. Yang, C. Firacative, C.A. Puime, P. Escandon, S. Bertout, F. Roger, J. Xu
Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that
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Pathogenicity chromosome of Fusarium oxysporum f. sp. cepae Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-17 Kosei Sakane, Mitsunori Akiyama, Sudisha Jogaiah, Shin-ichi Ito, Kazunori Sasaki
Fusarium oxysporum f. sp. cepae (Foc) is the causative agent of Fusarium basal rot disease in onions, which leads to catastrophic global crop production losses. Therefore, the interaction of Foc with its host has been actively investigated, and the pathogen-specific (PS) regions of the British strain Foc_FUS2 have been identified. However, it has not been experimentally determined whether the identified
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Unidirectional mating-type switching is underpinned by a conserved MAT1 locus architecture Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-17 P. Markus Wilken, Frances A. Lane, Emma T. Steenkamp, Michael J. Wingfield, Brenda D. Wingfield
Unidirectional mating-type switching is a form of homothallic reproduction known only in a small number of filamentous ascomycetes. Their ascospores can give rise to either self-sterile isolates that require compatible partners for subsequent sexual reproduction, or self-fertile individuals capable of completing this process in isolation. The limited studies previously conducted in these fungi suggest
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Sequence capture identifies fastidious chytrid fungi directly from host tissue Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-13 Kevin P. Mulder, Anna E. Savage, Brian Gratwicke, Joyce E. Longcore, Ed Bronikowski, Matthew Evans, Ana V. Longo, Naoko P. Kurata, Tim Walsh, Frank Pasmans, Nancy McInerney, Suzan Murray, An Martel, Robert C. Fleischer
The chytrid fungus Batrachochytrium dendrobatidis (Bd) was discovered in 1998 as the cause of chytridiomycosis, an emerging infectious disease causing mass declines in amphibian populations worldwide. The rapid population declines of the 1970s-1990s were likely caused by the spread of a highly virulent lineage belonging to the Bd-GPL clade that was introduced to naïve susceptible populations. Multiple
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CfErp3 regulates growth, conidiation, inducing ipomeamarone and the pathogenicity of Ceratocystis fimbriata Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-12-02 Changgen Li, Hao Cong, Xiaoying Cao, Yong Sun, Kailun Lu, Ludan Li, Yiming Wang, Yongjing Zhang, Qiang Li, Jihong Jiang, Lianwei Li
The Erp3 protein, which is an important member of the p24 family, is primarily responsible for the transport of cargo from the ER to the Golgi apparatus in Saccharomyces cerevisiae. However, the function of Erp3 in plant pathogenic fungi has not been reported. In this study, we characterized the ERP3 gene in Ceratocystis fimbriata, which causes the devastating disease sweetpotato black rot. The ΔCferp3
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Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-11-29 Vanessa Brito de Souza Rabello, Marcus de Melo Teixeira, Wieland Meyer, Laszlo Irinyi, Melissa Orzechowski Xavier, Vanice Rodrigues Poester, José Guillermo Pereira Brunelli, Fernando Almeida-Silva, Andrea Reis Bernardes‑Engemann, Isabella Dib Ferreira Gremião, Débora Ferreira dos Santos Angelo, Inácio José Clementino, Rodrigo Almeida-Paes, Rosely Maria Zancopé-Oliveira
Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to
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Polymorphisms at amino acid positions 85 and 86 in succinate dehydrogenase subunit C of Colletotrichum siamense: Implications for fitness and intrinsic sensitivity to SDHI fungicides Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-11-19 Yurong Qiu, Yaling Meng, Wenxu Lian, Shasha Jian, Yannan Du, Meng Wang, Ye Yang, Xiaoyu Liang, Yu Zhang
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Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-11-03 TingTing Hu, Jingjing Wang, Mianhui Chen, Lin Lin, Wei Wei, Dongzhi Wei
Penicillium brevicompactum is a critical industrial strain for the production of mycophenolic acid (MPA). However, the genetic background of Penicillium brevicompactum is unclear, and there are few tools available for genetic manipulation. To investigate its gene function, we first verified the feasibility of a pair of citrate synthase promoter (Pcit) and terminator (Tcit) from P. brevicompactum by
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A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-10-05 Terry W. Hill, Stanley Vance, Jennifer F. Loome, Benard J. Haugen, Darlene M. Loprete, Shana V. Stoddard, Loretta Jackson-Hayes
The calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown
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Genome-wide survey and evolutionary history of the pectin methylesterase (PME) gene family in the Dothideomycetes class of fungi Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-10-04 Vinicius Delgado da Rocha, Thaís Carolina da Silva Dal'Sasso, Maximiller Dal-Bianco, Luiz Orlando de Oliveira
Once deposited in the plant cell wall, pectin undergoes demethylesterification by endogenous pectin methylesterases (PMEs), which play various roles in growth and development, including defense against pathogen attacks. Pathogen PMEs can alter pectin's methylesterification pattern, increasing its susceptibility to degradation by other fungal pectinases and thus playing a critical role as virulence
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Convergent reductive evolution and host adaptation in Mycoavidus bacterial endosymbionts of Mortierellaceae fungi Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-15 Kevin Amses, Alessandro Desiró, Abigail Bryson, Igor Grigoriev, Stephen Mondo, Anna Lipzen, Kurt LaButti, Robert Riley, Vasanth Singan, Paris Salazar-Hamm, Jason King, Elizabeth Ballou, Teresa Pawlowska, Rasheed Adeleke, Gregory Bonito, Jessie Uehling
Intimate associations between fungi and intracellular bacterial endosymbionts are becoming increasingly well understood. Phylogenetic analyses demonstrate that bacterial endosymbionts of Mucoromycota fungi are related either to free-living Burkholderia or Mollicutes species. The so-called Burkholderia-related endosymbionts or BRE comprise Mycoavidus, Mycetohabitans and Candidatus Glomeribacter gigasporarum
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The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-18 Xenia Georgiou, Sofia Dimou, George Diallinas, Martina Samiotaki
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The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-16 Dianiris Luciano-Rosario, Omer Barda, Joanna Tannous, Dean Frawley, Özgür Bayram, Dov Prusky, Edward Sionov, Nancy P. Keller
Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found
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Conserved copper regulation of the antimicrobial isocyanide brassicicolin A in Alternaria brassicicola Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-12 Nischala Nadig, Sung Chul Park, Jin Woo Bok, Nancy P. Keller
Phytopathogenic Alternaria species are renown for production of toxins that contribute to virulence on host plants. Typically, these toxins belong to well-known secondary metabolite chemical classes including polyketides, non-ribosomal peptides and terpenes. However, the purported host toxin brassicicolin A produced by A. brassicicola is an isocyanide, a chemical class whose genetics and encoding gene
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The KdmB-EcoA-RpdA-SntB (KERS) chromatin regulatory complex controls development, secondary metabolism and pathogenicity in Aspergillus flavus Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-03 Betim Karahoda, Brandon T. Pfannenstiel, Özlem Sarikaya-Bayram, Zhiqiang Dong, Koon Ho Wong, Alastair B. Fleming, Nancy P. Keller, Özgür Bayram
The filamentous fungus Aspergillus flavus is a plant and human pathogen predominantly found in the soil as spores or sclerotia and is capable of producing various secondary metabolites (SM) such as the carcinogenic mycotoxin aflatoxin. Recently, we have discovered a novel nuclear chromatin binding complex (KERS) that contains the JARID1-type histone demethylase KdmB, a putative cohesion acetyl transferase
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Update on the state of research to manage Fusarium head blight Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-09-02 Soumya Moonjely, Malaika Ebert, Drew Paton-Glassbrook, Zachary A. Noel, Ludmila Roze, Rebecca Shay, Tara Watkins, Frances Trail
Fusarium head blight (FHB) is one of the most devastating diseases of cereal crops, causing severe reduction in yield and quality of grain worldwide. In the United States, the major causal agent of FHB is the mycotoxigenic fungus, Fusarium graminearum. The contamination of grain with mycotoxins, including deoxynivalenol and zearalenone, is a particularly serious concern due to its impact on the health
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A reference transcriptome for walnut anthracnose pathogen, Ophiognomonia leptostyla, guides the discovery of candidate virulence genes Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-08-31 Fatemeh Khelghatibana, Mohammad Javan-Nikkhah, Naser Safaie, Ahmad Sobhani, Somayeh Shams, Ehsan Sari
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The Bcvic1 and Bcvic2 vegetative incompatibility genes in Botrytis cinerea encode proteins with domain architectures involved in allorecognition in other filamentous fungi Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-08-26 Saadiah Arshed, Murray P. Cox, Ross E. Beever, Stephanie L. Parkes, Michael N. Pearson, Joanna K. Bowen, Matthew D. Templeton
Vegetative incompatibility is a fungal allorecognition system characterised by the inability of genetically distinct conspecific fungal strains to form a viable heterokaryon and is controlled by multiple polymorphic loci termed vic (vegetative incompatibility) or het (heterokaryon incompatibility). We have genetically identified and characterised the first vic locus in the economically important, plant-pathogenic
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Characterization of the need for galactofuranose during the Neurospora crassa life cycle Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-08-02 Hayden Schaff, Protyusha Dey, Christian Heiss, Griffin Keiser, Tatiana Rojo Moro, Parastoo Azadi, Pavan Patel, Stephen J. Free
Galactofuranose is a constituent of the cell walls of filamentous fungi. The galactofuranose can be found as a component of N-linked oligosaccharides, in O-linked oligosaccharides, in GPI-anchored galactomannan, and in free galactomannan. The Neurospora genome contains a single UDP-galactose mutase gene (ugm-1/NCU01824) and two UDP-galactofuranose translocases used to import UDP-galactofuranose into
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The RasGEF MoCdc25 regulates vegetative growth, conidiation and appressorium-mediated infection in the rice blast fungus Magnaporthe oryzae Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-07-17 Yu Xiao, Wuyun Lv, Qi Tong, Zhe Xu, Zhengyi Wang
Ras guanine nucleotide exchange factors (RasGEFs) can trigger Ras GTPase activities and play important roles in controlling various cellular processes in eukaryotes. Recently, it has been exhibited that RasGEF Cdc25 regulates morphological differentiation and pathogenicity in several plant pathogenic fungi. However, the role of RasGEFs in Magnaporthe oryzae is largely unknown. In this study, we identified
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Analysis of the emerging physical network in young mycelia Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-07-14
Filamentous fungi develop intricate hyphal networks that support mycelial foraging and transport of resources. These networks have been analyzed recently using graph theory, enabling the development of models that seek to predict functional traits. However, attention has focused mainly on mature colonies. Here, we report the extraction and analysis of the graph corresponding to Trichoderma atroviride
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The small Ras-like GTPase BUD-1 modulates conidial germination and hyphal growth guidance in the filamentous fungus Neurospora crassa Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-07-16 Nallely Cano-Domínguez, Olga A. Callejas-Negrete, Luis L. Pérez-Mozqueda, Juan M. Martínez-Andrade, Diego L. Delgado-Álvarez, Ernestina Castro-Longoria
In filamentous fungi, the hypha orientation is essential for polarized growth and morphogenesis. The ability to re-orient tip growth in response to environmental cues is critical for the colony survival. Therefore, hyphal tip orientation and tip extension are distinct mechanisms that operate in parallel during filamentous growth. In yeast, the axial growth orientation requires a pathway regulated by
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Deep amplicon sequencing reveals extensive allelic diversity in the erg11/CYP51 promoter and allows multi-population DMI fungicide resistance monitoring in the canola pathogen Leptosphaeria maculans Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-06-19 Jack L. Scanlan, Angela C. Mitchell, Stephen J. Marcroft, Leanne M. Forsyth, Alexander Idnurm, Angela P. Van de Wouw
Continued use of fungicides provides a strong selection pressure towards strains with mutations to render these chemicals less effective. Previous research has shown that resistance to the demethylation inhibitor (DMI) fungicides, which target ergosterol synthesis, in the canola pathogen Leptosphaeria maculans has emerged in Australia and Europe. The change in fungicide sensitivity of individual isolates
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Epiphytic proliferation of Zymoseptoria tritici isolates on resistant wheat leaves Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-06-19 H.N. Fones, D. Soanes, S.J. Gurr
The wheat pathogen Zymoseptoria tritici is capable of a long period of pre-invasive epiphytic growth. Studies have shown that virulent isolates vary in the extent, duration and growth form of this epiphytic growth, and the fungus has been observed to undergo behaviours such as asexual reproduction by budding and vegetative fusion of hyphae on the leaf surface. This epiphytic colonisation has been investigated
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The C2H2-type zinc-finger regulator AoKap5 is required for the growth and kojic acid synthesis in Aspergillus oryzae Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-19 Yuzhen Li, Ziming Chen, Feng Zhang, Tianming Chen, Junxia Fan, Xin Deng, Xiaocui Lei, Bin Zeng, Zhe Zhang
Aspergillus oryzae is an important filamentous fungus widely used for the industrial production of fermented foods and secondary metabolites. The clarifying of the mechanism of the growth and secondary metabolites in A. oryzae is important for its industrial production and utilization. Here, the C2H2-type zinc-finger protein AoKap5 was characterized to be involved in the growth and kojic acid production
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The evolution and ecology of psilocybin in nature Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-18 Matthew Meyer, Jason Slot
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Whole genome resequencing reveal patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-15 Xianbao Liu, Boxun Li, Jimiao Cai, Tao Shi, Yang Yang, Yanli Feng, Guixiu Huang
The Colletotrichum acutatum species complex possesses a diverse number of important traits, such as a wide host range and host preference, different modes of reproduction, and different strategies of host infection. Research using comparative genomics has attempted to find correlations between these traits. Here, we used multi-locus techniques and gene genealogical concordance analysis to investigate
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A transcription factor and a phosphatase regulate temperature-dependent morphogenesis in the fungal plant pathogen Zymoseptoria tritici Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-15 Carolina Sardinha Francisco, Bruce A. McDonald, Javier Palma-Guerrero
Naturally fluctuating temperatures provide a constant environmental stress that requires adaptation. Some fungal pathogens respond to heat stress by producing new morphotypes that maximize their overall fitness. The fungal wheat pathogen Zymoseptoria tritici responds to heat stress by switching from its yeast-like blastospore form to hyphae or chlamydospores. The regulatory mechanisms underlying this
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Redox perturbations in yeast cells lacking glutathione reductase Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-10 Agnieszka Janeczko, Michał Przywara, Roman Maslanka, Barbara Raś, Klaudia Ziaja, Magdalena Kwolek-Mirek, Renata Zadrag-Tecza, Sabina Bednarska
Cellular redox homeostasis has a major effect on cell functions and its maintenance is supported by glutathione and protein thiols which serve as redox buffers in cells. The regulation of the glutathione biosynthetic pathway is a focus of a lot of scientific research. However, still little is known about how complex cellular networks influence glutathione homeostasis. In this work was used an experimental
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Improved gene-targeting efficiency upon starvation in Saccharomycopsis Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-09 Davies Kaimenyi, Mareike Rij, Jürgen Wendland
Commonly used fungal transformation protocols rely on the use of either electroporation or the lithium acetate/single strand carrier DNA/Polyethylene glycol/heat shock method. We have used the latter method previously in establishing DNA-mediated transformation in Saccharomycopsis schoenii, a CTG-clade yeast that exhibits necrotrophic mycoparasitism. To elucidate the molecular mechanisms of predation
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Comparative proteomic analysis of two divergent strains provides insights into thermotolerance mechanisms of Ganoderma lingzhi Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-04 Manjun Cai, Xiaoxian Wu, Xiaowei Liang, Huiping Hu, Yuanchao Liu, Tianqiao Yong, Xiangmin Li, Chun Xiao, Xiong Gao, Shaodan Chen, Yizhen Xie, Qingping Wu
Heat stress (HS) is a major abiotic factor influencing fungal growth and metabolism. However, the genetic basis of thermotolerance in Ganoderma lingzhi (G. lingzhi) remains largely unknown. In this study, we investigated the thermotolerance capacities of 21 G. lingzhi strains and screened the thermo-tolerant (S566) and heat-sensitive (Z381) strains. The mycelia of S566 and Z381 were collected and subjected
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Detection and quantification of the histone code in the fungal genus Aspergillus Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-05-03 Xin Zhang, Roberta Noberini, Alessandro Vai, Tiziana Bonaldi, Michael F. Seidl, Jérȏme Collemare
In eukaryotes, the combination of different histone post-translational modifications (PTMs) – the histone code – impacts the chromatin organization as compact and transcriptionally silent heterochromatin or accessible and transcriptionally active euchromatin. Although specific histone PTMs have been studied in fungi, an overview of histone PTMs and their relative abundance is still lacking. Here, we
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First report on the regulation and function of carbon metabolism during large sclerotia formation in medicinal fungus Wolfiporia cocos Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-04-28 Cong Zhang, Lianfu Chen, Mengting Chen, Zhangyi Xu
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Characterization of the Trr/Trx system in the fungal pathogen Candida glabrata Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-04-25 Guadalupe Gutiérrez-Escobedo, Norma Vázquez-Franco, Ana López-Marmolejo, Gabriel Luna-Arvizu, Israel Cañas-Villamar, Irene Castaño, Alejandro De Las Peñas
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Love in the time of climate change: A review of sexual reproduction in the order Onygenales Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-04-24 Klaire Laux, Marcus de Melo Teixeira, Bridget Barker
Life-threatening infections caused by fungi in the order Onygenales have been rising over the last few decades. Increasing global temperature due to anthropogenic climate change is one potential abiotic selection pressure that may explain the increase in infections. The generation of genetically novel offspring with novel phenotypes through the process of sexual recombination could allow fungi to adapt
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A small cysteine-rich fungal effector, BsCE66 is essential for the virulence of Bipolaris sorokiniana on wheat plants Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-04-12 Vemula Chandra Kaladhar, Yeshveer Singh, Athira Mohandas Nair, Kamal Kumar, Achuit Kumar Singh, Praveen Kumar Verma
The Spot Blotch (SB) caused by hemibiotrophic fungal pathogen Bipolaris sorokiniana is one of the most devastating wheat diseases leading to 15–100% crop loss. However, the biology of Triticum-Bipolaris interactions and host immunity modulation by secreted effector proteins remain underexplored. Here, we identified a total of 692 secretory proteins including 186 predicted effectors encoded by B. sorokiniana
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Proteomic dissection of the role of GliZ in gliotoxin biosynthesis in Aspergillus fumigatus Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-04-05 Aimee M. Traynor, Özlem Sarikaya-Bayram, Özgür Bayram, José Antonio Calera, Sean Doyle
Gliotoxin (GT) biosynthesis in fungi is encoded by the gli biosynthetic gene cluster. While GT addition autoinduces biosynthesis, Zn2+ has been shown to attenuate cluster activity, and it was speculated that identification of Zn2Cys6 binuclear transcription factor GliZ binding partners might provide insight into this observation. Using the Tet-ON induction system, doxycycline (DOX) presence induced
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Population structure of Pyricularia oryzae on rice in Vietnam reveals diversified populations with four pandemic and two endemic clusters Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-03-30 Lieu Thi Le, Henri Adreit, Loan Thi Ha, Joelle Milazzo, Michel Lebrun, Didier Tharreau, Xuan Hoi Pham, Hai Thanh Nguyen, Elisabeth Fournier, Giang Thi Hoang
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Analysis of gene expression related to polyamine concentration and dimorphism induced in ornithine decarboxylase (odc) and spermidine synthase (spd) Ustilago maydis mutants Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-03-28 Fernando Pérez-Rodríguez, Laura Valdés-Santiago, Jorge Noé García-Chávez, José Luis Castro-Guillén, José Ruiz-Herrera
Polyamines are ubiquitous small organic cations, and their roles as regulators of several cellular processes are widely recognized. They are implicated in the key stages of the fungal life cycle. Ustilago maydis is a phytopathogenic fungus, the causal agent of common smut of maize and a model system to understand dimorphism and virulence. U. maydis grows in yeast form at pH 7 and it can develop its
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Functional genome annotation and transcriptome analysis of Pseudozyma hubeiensis BOT-O, an oleaginous yeast that utilizes glucose and xylose at equal rates Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-03-03 Friederike Mierke, Daniel P. Brink, Joakim Norbeck, Verena Siewers, Thomas Andlid
Pseudozyma hubeiensis is a basidiomycete yeast that has the highly desirable traits for lignocellulose valorisation of being equally efficient at utilization of glucose and xylose, and capable of their co-utilization. The species has previously mainly been studied for its capacity to produce secreted biosurfactants in the form of mannosylerythritol lipids, but it is also an oleaginous species capable
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DhFIG_2, a gene of nematode-trapping fungus Dactylellina haptotyla that encodes a component of the low-affinity calcium uptake system, is required for conidiation and knob-trap formation Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-02-26 Xiaozhou Zhao, Yani Fan, Weiwei Zhang, Meichun Xiang, Seogchan Kang, Shunxian Wang, Xingzhong Liu
Calcium ion (Ca2+) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca2+ from extracellular environments under high extracellular Ca2+ concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins
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The transcriptional activator ClrB is crucial for the degradation of soybean hulls and guar gum in Aspergillus niger Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-02-17 Roland S. Kun, Sandra Garrigues, Mao Peng, Keykhosrow Keymanesh, Anna Lipzen, Vivian Ng, Sravanthi Tejomurthula, Igor V. Grigoriev, Ronald P. de Vries
Low-cost plant substrates, such as soybean hulls, are used for various industrial applications. Filamentous fungi are important producers of Carbohydrate Active enZymes (CAZymes) required for the degradation of these plant biomass substrates. CAZyme production is tightly regulated by several transcriptional activators and repressors. One such transcriptional activator is CLR-2/ClrB/ManR, which has
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The small heat shock protein Hsp12.1 has a major role in the stress response and virulence of Cryptococcus gattii Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-02-11 Heryk Motta, Júlia Catarina Vieira Reuwsaat, Eamim Daidrê Squizani, Matheus da Silva Camargo, Ane Wichine Acosta Garcia, Augusto Schrank, Marilene Henning Vainstein, Charley Christian Staats, Lívia Kmetzsch
Cryptococcus gattii is one of the etiological agents of cryptococcosis. To achieve a successful infection, C. gattii cells must overcome the inhospitable host environment and deal with the highly specialized immune system and poor nutrients availability. Inside the host, C. gattii uses a diversified set of tools to maintain homeostasis and establish infection, such as the expression of remarkable and
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Predicting functions of putative fungal sesquiterpene synthase genes based on multiomics data analysis Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-01-24 Tetyana Nosenko, Ina Zimmer, Andrea Ghirardo, Tobias G. Köllner, Baris Weber, Andrea Polle, Maaria Rosenkranz, Jörg-Peter Schnitzler
Sesquiterpenes (STs) are secondary metabolites, which mediate biotic interactions between different organisms. Predicting the species-specific ST repertoires can contribute to deciphering the language of communication between organisms of the same or different species. High biochemical plasticity and catalytic promiscuity of sesquiterpene synthases (STSs), however, challenge the homology-based prediction
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Nanosized extracellular vesicles released by Neurospora crassa hyphae Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-01-21 Elizabeth Medina-Castellanos, Daniel A. Salgado-Bautista, Juan M. Martínez-Andrade, Ruben Dario Cadena-Nava, Meritxell Riquelme
Extracellular vesicles (EVs) are nanosized structures containing proteins, lipids, and nucleic acids, released by living cells to the surrounding medium. EVs participate in diverse processes, such as intercellular communication, virulence, and disease. In pathogenic fungi, EVs carry enzymes that allow them to invade the host or undergo environmental adaptation successfully. In Neurospora crassa, a
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The establishment of multiple knockout mutants of Colletotrichum orbiculare by CRISPR-Cas9 and Cre-loxP systems Fungal Genet. Biol. (IF 3.0) Pub Date : 2023-01-18 Kohji Yamada, Toya Yamamoto, Kanon Uwasa, Keishi Osakabe, Yoshitaka Takano
Colletotrichum orbiculare is employed as a model fungus to analyze molecular aspects of plant-fungus interactions. Although gene disruption via homologous recombination (HR) was established for C. orbiculare, this approach is laborious due to its low efficiency. Here we developed methods to generate multiple knockout mutants of C. orbiculare efficiently. We first found that CRISPR-Cas9 system massively