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Identification of a new gregarine parasite associated with mass mortality events of freshwater pearl mussels (Margaritifera margaritifera) in Sweden J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-03-14 Anders Alfjorden, Ioana Onut‐Brännström, Niklas Wengström, Arni Kristmundsson, Mahwash Jamy, B. David Persson, Fabien Burki
Freshwater bivalves play key ecological roles in lakes and rivers, largely contributing to healthy ecosystems. The freshwater pearl mussel, Margaritifera margaritifera, is found in Europe and on the East coast of North America. Once common in oxygenated streams, M. margaritifera is rapidly declining and consequently assessed as a threatened species worldwide. Deterioration of water quality has been
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Continuous reproduction of planktonic foraminifera in laboratory culture J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-02-25 Julie Meilland, Michael Siccha, Raphaël Morard, Michal Kucera
Planktonic foraminifera were long considered obligate sexual outbreeders but recent observations have shown that nonspinose species can reproduce by multiple fission. The frequency of multiple fission appears low but the survival rate of the offspring is high and specimens approaching fission can be distinguished. We made use of this observation and established a culturing protocol aimed at enhancing
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Novel cytoskeletal traits in the intestinal parasites (Squirmida, Platyproteum vivax) of Pacific peanut worms (Sipuncula, Phascolosoma agassizii) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-02-25 Danja Currie‐Olsen, Brian S. Leander
The cytoskeletal organization of a squirmid, namely Platyproteum vivax, was investigated with confocal laser scanning microscopy (CLSM) to refine inferences about convergent evolution among intestinal parasites of marine invertebrates. Platyproteum inhabits Pacific peanut worms (Phascolosoma agassizii) and has traits that are similar to other lineages of myzozoan parasites, namely gregarine apicomplexans
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Living in the cracks: Two novel genera of Variosea (Amoebozoa) discovered on an urban sidewalk J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-01-19 Nicholas Fry, Gabriel A. Schuler, Robert E. Jones, Peter G. Kooienga, Violet Jira, Maggie Shepherd, Alexander K. Tice, Matthew W. Brown
Biological soil crusts represent a rich habitat for diverse and complex eukaryotic microbial communities. A unique but extremely common habitat is the urban sidewalk and its cracks that collect detritus. While these habitats are ubiquitous across the globe, little to no work has been conducted to characterize protists found there. Amoeboid protists are major predators of bacteria and other microbial
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First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal-keepers in Ilam, western Iran: A zoonotic concern J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-01-15 Morteza Shams, Ali Mohammad Bahrami, Asma Mousivand, Laya Shamsi, Ali Asghari, Saeed Shahabi, Alireza Sadrebazzaz
A total of 360 fecal samples were randomly collected from 150 cattle, 150 sheep, and 60 humans (30 people with close animal contact and 30 individuals without close animal contact) at 10 farms in Ilam, western Iran from June 2022 to August 2023. All samples were directly examined for Blastocystis by zinc sulfate flotation, followed by microscopic observation. Positive samples were further subtyped
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Grazing preferences of three species of amoebae on cyanobacteria and green algae J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2024-01-10 Harold G. Weger, April K. Polasek, Derek M. Wright, Arun Damodaran, John Stavrinides
Twenty species/isolates of cyanobacteria and green algae were isolated from cyanobacterial bloom samples in lakes associated with the upper Qu'Appelle River drainage system in southern Saskatchewan, Canada. Three amoebae species (Cochliopodium sp., Vannella sp. and Vermamoeba vermiformis) were also isolated from one of these samples, and were subjected to grazing assays to determine which species of
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The function of the feeding groove of ‘typical excavate’ flagellates J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-12-18 Sei Suzuki-Tellier, Thomas Kiørboe, Alastair G. B. Simpson
Phagotrophic flagellates are the main consumers of bacteria and picophytoplankton. Despite their ecological significance in the ‘microbial loop’, many of their predation mechanisms remain unclear. ‘Typical excavates’ bear a ventral groove, where prey is captured for ingestion. The consequences of feeding through a ‘semi-rigid’ furrow on the prey size range have not been explored. An unidentified moving
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The second most abundant dinophyte in the ponds of a botanical garden is a species new to science J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-12-11 Anna Müller, Marina Stark, Sophia Schottenhammel, Uwe John, Juliana Chacón, Andreas Klingl, Victoria Julia Christine Holzer, Marika Schöffer, Marc Gottschling
In the microscopy realm, a large body of dark biodiversity still awaits to be uncovered. Unarmoured dinophytes are particularly neglected here, as they only present inconspicuous traits. In a remote German locality, we collected cells, from which a monoclonal strain was established, to study morphology using light and electron microscopy and to gain DNA sequences from the rRNA operon. In parallel,
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Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-12-10 Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai
Long noncoding RNAs (lncRNAs) are regulatory transcripts during protozoan infections in the host intestinal epithelial cells (IECs). Apicomplexan Eimeria falciformis sporozoite extracellular vesicles (EVs) contain virulence factors that modulate host IECs pro-inflammatory genes and immune responses. In this study, E. falciformis sporozoites were made to interact with inactivated host cells, and the
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Correction to the article “On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle” J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-12-08
Drachko, D., Shishkin, Y. & Zlatogursky, V.V. (2022): On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle, 69:e12916. https://doi.org/10.1111/jeu.12916. The authors would like to revise the Acknowledgment section in the article. The corrected statement is as below,
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In memoriam: Thomas Cavalier-Smith (1942–2021) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-12-07 Gaytha A. Langlois, Sonja Rueckert
Thomas Cavalier-Smith, born in London, U.K., on October 21, 1942, was a Professor of Evolutionary Biology in the Department of Zoology at the University of Oxford at the time of his death on March 19, 2021. Credited with at least 235 research works and over 20,000 citations, Cavalier-Smith was a well-known and widely respected scientist who took a bold and detailed approach to understanding major transitions
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Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-11-29 Xiaochen Feng, Daniel Méndez-Sánchez, Wenbao Zhuang, Ran Li, Ondřej Pomahač, Ivan Čepička, Johana Rotterová, Xiaozhong Hu
The morphology, morphogenesis, and molecular phylogeny of a new metopid ciliate, Castula specialis sp. nov., comprising three strains from geographically distant (China, Mexico, Czech Republic) anoxic freshwater habitats, were studied based on microscopic observation of live and protargol-stained specimens as well as SSU rRNA gene sequence data. The new species is characterized as follows: size in vivo
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Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-11-17 Xinwei Feng, Luyao Xin, Bowen Zhang, Zhichao Wang, Zhu Meng, Fuchang Yu, Meng Qi
To determine the infection status and assess the zoonotic potential of Blastocystis spp. in Hotan Black chickens in southern Xinjiang, China, fecal samples were collected from 617 chickens on 18 large-scale farms. The presence of Blastocystis spp. was determined using polymerase chain reaction based on the small subunit rRNA (SSU rRNA) locus. The results revealed an overall infection rate of 26.3%
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Rhabdamoeba marina is a heterotrophic relative of chlorarachnid algae J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-11-08 Takashi Shiratori, Ken-ichiro Ishida
Rhabdamoeba marina is a unique and poorly reported amoeba with an uncertain phylogenetic position. We successfully cultured R. marina from coastal seawater in Japan and performed a molecular phylogenetic analysis using the small subunit ribosomal RNA (SSU rRNA) gene sequence. Our phylogenetic analysis showed that R. marina branched as a basal lineage of Chlorarachnea, a group of marine photosynthetic
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Anaeramoeba pumila sp. nov. and Anaeramoeba sp. OCE22C represent two novel types of symbiosis of Anaeramoebae and prokaryotes J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-11-06 Magdaléna Pavlátová, Pavla Hanousková, Ivan Čepička
Anaeramoebae is a recently described phylum of anaerobic, marine amoebae, and amoeboflagellates belonging to the Metamonada supergroup. So far, six species have been described based on light microscopic morphology and sequences of the SSU rRNA gene. Here we present three new strains of Anaeramoeba with a description of their morphology, ultrastructure, and phylogenetic position based on the analysis
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Highly divergent morphology but a close molecular phylogenetic relationship between two little-known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-10-27 Yong Chi, Danxu Tang, Jingtao Lei, Fan Wei, Saleh A. Al-Farraj, William A. Bourland, Zigui Chen
Free-living litostomatean ciliates, prominent microeukaryote predators commonly encountered in freshwater and marine habitats, play vital roles in maintaining energy flow and nutrient cycles. Nevertheless, understanding their biodiversity and phylogenetic relationships remains challenging due to insufficient morphological information and molecular data. As a new contribution to this group, three haptorian
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Single-cell DNA from West Greenland marine sediments suggests presence of Protoperidinium tricingulatum in the Arctic J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-10-25 Tiia Luostarinen, Sofia Ribeiro, Heike H. Zimmermann, Anna B. Kvorning, Maija Heikkilä
Spiny brown dinoflagellate cysts are commonly used as sea-ice indicators in the Arctic, but their biological affinities are not well known. We present the first indication of hitherto temperate Protoperidinium tricingulatum in the Arctic based on single-cell LSU rDNA sequencing from sediments of the Disko Bay-Vaigat Sound, West Greenland. The morphological similarity of the sequenced cyst morphotype
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Paramecium (Oligohymenophorea, Ciliophora) diversity in Thailand sheds light on the genus biogeography and reveals new phylogenetic lineages J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-10-17 Maksim Melekhin, Alexey Potekhin, Eleni Gentekaki, Chitchai Chantangsi
Paramecium (Ciliophora, Oligohymenophorea) is a good model to study ciliate biogeography. Extensive sampling mainly in northern hemisphere has led to 16 valid morphological species description thus far. However, a majority of hard-to-reach regions, including South East Asia, are underinvestigated. Our study combined traditional morphological and molecular approaches to reveal the biodiversity of Paramecium
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Kaonashia insperata gen. et sp. nov., a eukaryotrophic flagellate, represents a novel major lineage of heterotrophic stramenopiles J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-10-06 Elizabeth J. Weston, Yana Eglit, Alastair G. B. Simpson
Eukaryotrophic protists are ecologically significant and possess characteristics key to understanding the evolution of eukaryotes; however, they remain poorly studied, due partly to the complexities of maintaining predator–prey cultures. Kaonashia insperata, gen. nov., et sp. nov., is a free-swimming biflagellated eukaryotroph with a conspicuous ventral groove, a trait observed in distantly related
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Pseudovampyrella gen. nov.: A genus of Vampyrella-like protoplast extractors finds its place in the Leptophryidae J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-09-25 Andreas Suthaus, Sebastian Hess
Vampyrellid amoebae are predatory protists, which consume a variety of eukaryotic prey and inhabit freshwater, marine and terrestrial ecosystems. Although they have been known for almost 150 years, much of their diversity lacks an in-depth characterization. To date, environmental sequencing data hint at several uncharacterized lineages, to which no phenotype is associated. Furthermore, there are numerous
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Trichite features contribute to the revision of the genus Strombidium (Alveolata, Ciliophora, Spirotricha) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-09-25 Sabine Agatha, Birgit Weißenbacher, Michael Kirschner, Maximilian H. Ganser
Strombidium is a species-rich genus of oligotrichid ciliates mainly inhabiting the marine pelagial. In molecular phylogenies, the genus emerged as non-monophyletic, and cladistic analyses suggest that it is largely characterized by plesiomorphies. A reliable split of the genus and the establishment of new genera necessitate, however, support by novel morphological and/or ultrastructural features. In
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Gene editing of putative cAMP and Ca2+-regulated proteins using an efficient cloning-free CRISPR/Cas9 system in Trypanosoma cruzi J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-09-19 Miguel A. Chiurillo, Milad Ahmed, César González, Aqsa Raja, Noelia Lander
Trypanosoma cruzi, the agent of Chagas disease, must adapt to a diversity of environmental conditions that it faces during its life cycle. The adaptation to these changes is mediated by signaling pathways that coordinate the cellular responses to the new environmental settings. Cyclic AMP (cAMP) and Calcium (Ca2+) signaling pathways regulate critical cellular processes in this parasite, such as differentiation
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Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-09-04 Emanuel Ceballos-Góngora, Julio César Torres-Romero, Victor Ermilo Arana-Argáez, María Elizbeth Alvarez-Sánchez, Karla Acosta-Viana, Antonio Euan-Canto, Leidi Cristal Alvarez-Sánchez
Tritrichomonas foetus is a flagellated parasite that primarily infects the reproductive tissues of livestock, causing bovine trichomoniasis. The cytoplasmic membrane of T. foetus contains various compounds that contribute to adherence, colonization, and pathogenicity. Metronidazole (MTZ) is the main treatment for trichomoniasis, but the emergence of drug-resistant strains is a concern due to improper
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Division of Blastocystis ST10 into three new subtypes: ST42-ST44 J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-09-01 Monica Santin, Ana Figueiredo, Aleksey Molokin, Nadja S George, Pamela C. Köster, Alejandro Dashti, David González-Barrio, David Carmena, Jenny G. Maloney
The Blastocystis subtype ST10 has been recognized to contain a great deal of diversity at the sequence level, potentially indicating the presence of multiple new STs within the clade. However, the data needed to validate these new STs were not available. To help resolve this diversity, full-length small subunit (SSU) rRNA gene reference sequences were generated using Oxford Nanopore MinION long-read
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Molecular and morphological characterization of four new ancyromonad genera and proposal for an updated taxonomy of the Ancyromonadida J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-08-22 Naoji Yubuki, Guifré Torruella, Luis Javier Galindo, Aaron A. Heiss, Maria Cristina Ciobanu, Takashi Shiratori, Ken-ichiro Ishida, Jazmin Blaz, Eunsoo Kim, David Moreira, Purificación López-García, Laura Eme
Ancyromonads are small biflagellated protists with a bean-shaped morphology. They are cosmopolitan in marine, freshwater, and soil environments, where they attach to surfaces while feeding on bacteria. These poorly known grazers stand out by their uncertain phylogenetic position in the tree of eukaryotes, forming a deep-branching “orphan” lineage that is considered key to a better understanding of
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Response of protists to nitrogen addition, arbuscular mycorrhizal fungi manipulation, and mesofauna reduction in a tropical montane rainforest in southern Ecuador J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-08-14 Garvin Schulz, Tessa Camenzind, Laura M. Sánchez-Galindo, Dominik Schneider, Stefan Scheu, Valentyna Krashevska
The tropical Andes are a species-rich and nitrogen-limited system, susceptible to increased nitrogen (N) inputs from the atmosphere. However, our understanding of the impacts of increased N input on belowground systems, in particular on protists and their role in nutrient cycling, remains limited. We explored how increased N affects protists in tropical montane rainforests in Ecuador using high-throughput
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Morphological and molecular characterization of a new member of the phylum Rhodelphidia J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-08-07 Kristina I. Prokina, Denis V. Tikhonenkov, Purificación López-García, David Moreira
Rhodelphidia is a recently discovered phylum within the supergroup Archaeplastida, comprising only two known representatives (Rhodelphis marinus and Rhodelphis limneticus). Despite its close phylogenetic relatedness to red algae, Rhodelphidia differ markedly by being nonphotosynthetic eukaryotrophic flagellates with gene- and intron-rich genomes. Here, we describe a new freshwater Rhodelphidia species
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AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form Trypanosoma brucei parasites J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-08-07 Jennifer A. Black, Christen M. Klinger, Leandro Lemgruber, Joel B. Dacks, Jeremy C. Mottram, Richard McCulloch
Selection and internalization of cargo via clathrin-mediated endocytosis requires adaptor protein complexes. One complex, AP-2, acts during cargo selection at the plasma membrane. African trypanosomes lack all components of the AP-2 complex, except for a recently identified orthologue of the AP-2-associated protein kinase 1, AAK1. In characterized eukaryotes, AAK1 phosphorylates the μ2 subunit of the
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Seasonal dynamics of eukaryotic microbial diversity in hypersaline Tuz Lake characterized by 18S rDNA sequencing J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-08-01 Suzan Şahin Doğan, Aytaç Kocabaş
Microbial diversity found in hypersaline ecosystems is structurally unique and essential in many microbiological and ecological processes. Tuz Lake, the second biggest lake in Türkiye, is a talassohaline (over 32% [w/v]) lake with near-neutral pH. The aim of study was to investigate the composition of the eukaryotic microbial community in Tuz Lake by 18S rDNA amplicon sequencing, as well as its relationship
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Unexpected ubiquity of heart-shaped scale morphotype in Centroplasthelida (Haptista): Ancestral trait or multiple acquisitions? J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-07-14 Elena A. Gerasimova, Yulia V. Mindolina, Denis V. Tikhonenkov, Vladimir Y. Kataev, Alexander S. Balkin, Kirill V. Mikhailov, Dmitry G. Zagumyonnyi, Andrey O. Plotnikov, Vasily V. Zlatogursky
Centrohelids (Haptista: Centroplasthelida) are axopodial protists with a remarkable diversity of external siliceous scale morphologies. It is believed that the last common ancestor of centrohelids had a double layer of siliceous scales composed of plate scales closer to a cell surface and spine scales radiating outwards. The characteristic morphotype of spine scales with a heart-shaped base was once
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Interpreting phylogenetic placements for taxonomic assignment of environmental DNA J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-07-13 Isabelle Ewers, Lubomír Rajter, Lucas Czech, Frédéric Mahé, Alexandros Stamatakis, Micah Dunthorn
Taxonomic assignment of operational taxonomic units (OTUs) is an important bioinformatics step in analyzing environmental sequencing data. Pairwise alignment and phylogenetic-placement methods represent two alternative approaches to taxonomic assignments, but their results can differ. Here we used available colpodean ciliate OTUs from forest soils to compare the taxonomic assignments of VSEARCH (which
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Molecular phylogenetics of sessile Dolium sedentarium, a petalomonad euglenid J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-07-09 Gordon Lax, Patrick J. Keeling
The euglenids are a species-rich group of flagellates with varying modes of nutrition that can be found in diverse habitats. Phagotrophic members of this group gave rise to phototrophs and hold the key to understanding the evolution of euglenids as a whole, including the evolution of complex morphological characters like the euglenid pellicle. Yet to understand the evolution of these characters, a
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Fine structure and molecular characterization of two new parabasalid species that naturally colonize laboratory mice, Tritrichomonas musculus and Tritrichomonas casperi J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-06-10 Leila Tuzlak, Eliza V. C. Alves-Ferreira, Cindi L. Schwartz, Andrea Kennard, Jacqueline M. Leung, Christina Shehata, Michael E. Grigg
Tritrichomonas muris is a common flagellated protist isolated from the cecum of wild rodents. This commensal protist has been shown previously to alter immune phenotypes in laboratory mice. Other trichomonads, referred to as Tritrichomonas musculis and Tritrichomonas rainier, also naturally colonize laboratory mice and cause immune alterations. This report formally describes two new trichomonads, Tritrichomonas
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True molecular phylogenetic position of the cockroach gut commensal Lophomonas blattarum (Lophomonadida, Parabasalia) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-06-08 LeAnn Nguyen, Stephen J. Taerum, Daniel E. Jasso-Selles, Claudio H. Slamovits, Jeffrey D. Silberman, Gillian H. Gile
Lophomonas blattarum is a facultative commensal gut dweller of common pest cockroaches. Its cells are roughly spherical in shape with an apical tuft of ~50 flagella. Controversially, it has been implicated in human respiratory infections based on light microscopic observations of similarly shaped cells in sputum or bronchoalveolar lavage fluid. Here, we have sequenced the 18S rRNA gene of L. blattarum
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New Parabasalia symbionts Snyderella spp. and Daimonympha gen. nov. from South American Rugitermes termites and the parallel evolution of a cell with a rotating “head” J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-06-06 Elisabeth Hehenberger, Vittorio Boscaro, Erick R. James, Yoshihisa Hirakawa, Morelia Trznadel, Mahara Mtawali, Rebecca Fiorito, Javier del Campo, Anna Karnkowska, Martin Kolisko, Nicholas A. T. Irwin, Varsha Mathur, Rudolf H. Scheffrahn, Patrick J. Keeling
Most Parabasalia are symbionts in the hindgut of “lower” (non-Termitidae) termites, where they widely vary in morphology and degree of morphological complexity. Large and complex cells in the class Cristamonadea evolved by replicating a fundamental unit, the karyomastigont, in various ways. We describe here four new species of Calonymphidae (Cristamonadea) from Rugitermes hosts, assigned to the genus
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Comparison between p-distance and single-locus species delimitation models for delineating reproductively tested strains of pennate diatoms (Bacillariophyceae) using cox1, rbcL and ITS J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-05-26 Andréa de O. da R. Franco, Matt P. Ashworth, Clarisse Odebrecht
Several automated molecular methods have emerged for distinguishing eukaryote species based on DNA sequence data. However, there are knowledge gaps around which of these single-locus methods is more accurate for the identification of microalgal species, such as the highly diverse and ecologically relevant diatoms. We applied genetic divergence, Automatic Barcode Gap Discovery for primary species delimitation
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On stomatocysts of Paraphysomonas caelifrica (Stramenopiles, Paraphysomonadida) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-05-22 Dmitry Kapustin, Marina Ignatenko, Tatyana Yatsenko-Stepanova
Stomatocysts of the rare heterotrophic chrysophyte, Paraphysomonas caelifrica, were discovered from a shallow ephemeral pond Tavolgasai (“Orenburgskiy” State Nature Reserve, Orenburg Region, Russia). Morphology of stomatocysts was studied using scanning electron microscopy. Stomatocysts of P. caelifrica are spherical and smooth with a cylindrical collar surrounding the regular pore. So, they do not
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Identification and validation of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-05-17 Carolina Hernández-Castro, Jenny G. Maloney, Sonia P. Agudelo-López, Miguel A. Toro-Londoño, Jorge H. Botero-Garcés, María C. Orozco, Yulieth C. Quintero-Quinchia, Juan C. Correa-Cote, Alejandro Múnera-Duque, Juan C. Ricaurte-Ciro, Luis I. Londoño-Álvarez, René M. Escobar, Pamela C. Köster, Sergio Sánchez, David Carmena, Mónica Santín
Blastocystis sp. is among the most frequent intestinal protists identified in humans globally. However, characterization of Blastocystis subtype diversity in humans is ongoing. We report here the identification of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening involving colonoscopy and fecal testing (microscopy, culture, PCR). The full-length ssu rRNA
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Vegetative cell fusion and a new stage in the life cycle of the Aphelida (Opisthosporidia) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-04-13 Victoria S. Tcvetkova, Igor R. Pozdnyakov, Alexei O. Seliuk, Natalia A. Zorina, Sergey A. Karpov
The aphelids, intracellular parasitoids of algae, represent a large cluster of species sister to Fungi in molecular phylogenetic trees. Sharing a common ancestor with Fungi, they are very important in terms of evolution of these groups of Holomycota. Aphelid life cycle being superficially similar to that of Chytridiomycetes is understudied. We have found in the aphelids a new stage—big multiflagellar
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The photic-aphotic divide is a strong ecological and evolutionary force determining the distribution of ciliates (Alveolata, Ciliophora) in the ocean J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-04-08 Luciana F. Santoferrara, Aleena Qureshi, Amina Sher, Leocadio Blanco-Bercial
The bulk of knowledge on marine ciliates is from shallow and/or sunlit waters. We studied ciliate diversity and distribution across epi- and mesopelagic oceanic waters, using DNA metabarcoding and phylogeny-based metrics. We analyzed sequences of the 18S rRNA gene (V4 region) from 369 samples collected at 12 depths (0–1000 m) at the Bermuda Atlantic Time-series Study site of the Sargasso Sea (North
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Through the eDNA looking glass: Responses of fjord benthic foraminiferal communities to contrasting environmental conditions J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-03-27 Inda Brinkmann, Magali Schweizer, David Singer, Sophie Quinchard, Christine Barras, Joan M. Bernhard, Helena L. Filipsson
The health of coastal marine environments is severely declining with global changes. Proxies, such as those based on microeukaryote communities, can record biodiversity and ecosystem responses. However, conventional studies rely on microscopic observations of limited taxonomic range and size fraction, missing putatively ecologically informative community components. Here, we tested molecular tools
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Phylogenomics of novel ploeotid taxa contribute to the backbone of the euglenid tree J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-03-13 Gordon Lax, Anna Cho, Patrick J. Keeling
Euglenids are a diverse group of flagellates that inhabit most environments and exhibit many different nutritional modes. The most prominent euglenids are phototrophs, but phagotrophs constitute the majority of phylogenetic diversity of euglenids. They are pivotal to our understanding of euglenid evolution, yet we are only starting to understand relationships amongst phagotrophs, with the backbone
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Correction to “Cascading effects of prey identity on gene expression in a kleptoplastidic ciliate” J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-03-12
Paight, C., Johnson, M.D., Lasek-Nesselquist, E. & Moeller, H.V. (2023) Cascading effects of prey identity on gene expression in a kleptoplastidic ciliate. Journal of Eukaryotic Microbiology, 70, e12940. Available from: https://doi.org/10.1111/jeu.12940 In the originally published version of the article, Figure S1 from the supporting information and Figure 1 from the main article were transposed. The
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The Mixoplankton Database (MDB): Diversity of photo-phago-trophic plankton in form, function, and distribution across the global ocean J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-27 Aditee Mitra, David A. Caron, Emile Faure, Kevin J. Flynn, Suzana Gonçalves Leles, Per J. Hansen, George B. McManus, Fabrice Not, Helga do Rosario Gomes, Luciana F. Santoferrara, Diane K. Stoecker, Urban Tillmann
Protist plankton are major members of open-water marine food webs. Traditionally divided between phototrophic phytoplankton and phagotrophic zooplankton, recent research shows many actually combine phototrophy and phagotrophy in the one cell; these protists are the “mixoplankton.” Under the mixoplankton paradigm, “phytoplankton” are incapable of phagotrophy (diatoms being exemplars), while “zooplankton”
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Functional ecology of planktonic ciliates: Measuring mortality rates in response to starvation J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-24 Thomas Weisse, Ulrike Scheffel, Peter Stadler
Population dynamics of aquatic ciliates are controlled “bottom-up” via food supply and “top-down” by grazing and parasitism. While intrinsic growth rates of ciliates under saturating food conditions have been studied in some detail, mortality rates induced by starvation have received little attention thus far. To this end, we examined the response of three algivorous freshwater ciliate species to starvation
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Phylogenetic placement of the protosteloid amoeba Microglomus paxillus identifies another case of sporocarpic fruiting in Discosea (Amoebozoa) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-24 Alexander K. Tice, Frederick W. Spiegel, Matthew W. Brown
Protosteloid amoebae are a paraphyletic assemblage of amoeboid protists found exclusively in the eukaryotic assemblage Amoebozoa. These amoebae can facultatively form a dispersal structure known as a fruiting body, or more specifically, a sporocarp, from a single amoeboid cell. Sporocarps consist of one to a few spores atop a noncellular stalk. Protosteloid amoebae are known in two out of three well-established
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Morphological and molecular diagnosis of two new species of Trypanosoma Gruby, 1843 infecting South African cordylid lizards (Squamata: Cordylidae: Cordylinae), Trypanosoma (Squamatrypanum) ndumoensis n. sp. and Trypanosoma (Trypanosoma) tokoloshi n. sp. J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-24 Bernard J. Jordaan, Johann van As, Edward C. Netherlands
Despite reptile trypanosomes forming a large group, the majority of species descriptions are data deficient, lacking key characteristic data and supporting molecular data. Reptile hosts show potential to facilitate transmission of zoonotic trypanosomiases and offer key information to understanding the genus of Trypanosoma. Several species of squamates from different localities in South Africa were
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Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha, Spirotrichonympha, and three new genera Pseudospironympha, Nanospironympha, and Brugerollina J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-09 Satoko Noda, Osamu Kitade, Daniel E. Jasso-Selles, Stephen J. Taerum, Miki Takayanagi, Renate Radek, Nathan Lo, Moriya Ohkuma, Gillian H. Gile
Spirotrichonymphea, one of the six classes of phylum Parabasalia, are characterized by bearing many flagella in spiral rows, and they occur exclusively in the guts of termites. Phylogenetic relationships among the 13 described genera are not well understood due to complex morphological evolution and a paucity of molecular data. One such understudied genus is Spironympha. It has been variously considered
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High-resolution phylogenetic analysis reveals long-term microbial dynamics and microdiversity in phytoplankton microbiome J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-08 Chang Jae Choi, Cecile Jauzein, Deana L. Erdner
Phytoplankton-bacteria interactions represent the evolution of complex cross-kingdom networks requiring niche specialization of diverse microbes. Unraveling this co-evolutionary process has proven challenging because microbial partnerships are complex, and their assembly can be dynamic as well as scale- and taxon-dependent. Here, we monitored long-term experimental evolution of phytoplankton-bacteria
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Morphology and phylogenetic position of three anaerobic ciliates from the classes Odontostomatea and Muranotrichea (Ciliophora) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-02-02 Daniel Méndez-Sánchez, Ondřej Pomahač, Johana Rotterová, William A. Bourland, Ivan Čepička
The diversity of the classes Odontostomatea and Muranotrichea, which contain solely obligate anaerobes, is poorly understood. We studied two populations of Mylestoma sp., one of Saprodinium dentatum (Odontostomatea), two of Muranothrix felix sp. nov., and one of Muranothrix sp. (Muranotrichea) employing live observation, protargol impregnation, scanning electron microscopy, and 18S rRNA gene sequencing
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Investigating the interactive effects of temperature, pH, and salinity on Naegleria fowleri persistence J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-01-29 Leigha M. Stahl, Julie B. Olson
Naegleria fowleri causes primary amoebic meningoencephalitis, a deadly infection that occurs when free-living amoebae enter the nose via freshwater and travel to the brain. N. fowleri naturally thrives in freshwater and soil and is thought to be associated with elevated water temperatures. While environmental and laboratory studies have sought to identify what environmental factors influence its presence
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Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-01-11 Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Trichomoniasis is a common and curable sexually transmitted disease worldwide. The rapid, convenient, and accurate diagnosis of trichomoniasis is an important link in the prevention and treatment of the disease. The current detection methods of Trichomonas vaginalis are mainly wet mount microscopy, culture, nested PCR, and loop-mediated isothermal amplification. However, these detection methods have
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Phaeogromids of the mesopelagic marine plankton: Temporal variability of concentrations and observations of feeding structures of four species from the mesopelagic in the Mediterranean Sea J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2023-01-05 John R. Dolan, Laurent Coppola
Challengerids, phaeogromids rhizarian protists, are emblematic protists of the deep sea but are also enigmatic as they occur in very low concentrations. In previous studies, we reported on temporal changes in abundance at a near-shore mesopelagic site, but only as part of sampling of the entire microplankton assemblage, not well-suited for examining phaeogromids. Consequently, we turned to using a
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Formation of multicellular colonies by choanoflagellates increases susceptibility to capture by amoeboid predators J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-12-28 Nicole E. Chin, Tiffany C. Wu, J. Michael O'Toole, Kevin Xu, Tom Hata, Mimi A. R. Koehl
Many heterotrophic microbial eukaryotes are size-selective feeders. Some microorganisms increase their size by forming multicellular colonies. We used choanoflagellates, Salpingoeca helianthica, which can be unicellular or form multicellular colonies, to study the effects of multicellularity on vulnerability to predation by the raptorial protozoan predator, Amoeba proteus, which captures prey with
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Molecular prevalence and subtypes distribution of Blastocystis sp. among outpatients and inpatients in north and south areas of Henan Province, China J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-12-07 Xuefang Mei, Changwei Su, Weijuan Wang, Bo Zhang, Lai Wei, Zhenchao Zhang, Xiaowei Tian, Zhenke Yang, Xiangrui Li, Aijun Duan, Shuai Wang
Blastocystis sp. is one of the most common intestinal parasites in humans and many animals. To further understand the infection of Blastocystis hominis (B. hominis) and the distribution of its genotype in some areas of Henan Province, China, 793 stool samples from outpatients and inpatients in Xinxiang City and Xinyang City, Henan Province were collected from April 2020 to July 2022. The samples were
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18S rDNA sequence–structure phylogeny of the Euglenophyceae (Euglenozoa, Euglenida) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-12-07 Antonia S. Rackevei, Anna Karnkowska, Matthias Wolf
The phylogeny of Euglenophyceae (Euglenozoa, Euglenida) has been discussed for decades with new genera being described in the last few years. In this study, we reconstruct a phylogeny using 18S rDNA sequence and structural data simultaneously. Using homology modeling, individual secondary structures were predicted. Sequence–structure data are encoded and automatically aligned. Here, we present a sequence–structure
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Novel insights into molecular mechanisms of vegetative cell cycle and resting cyst formation in Apodileptus cf. visscheri (Alveolata, Ciliophora) J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-12-02 Jiahui Xu, Zhuo Shen, Minjie Yu, Yalan Sheng, Zhenzhen Yi
Ciliates usually with big cell sizes, complex morphological structures, and diverse life cycles, are good model organisms for studying cell proliferation regulation of eukaryotes. Up to date, the molecular regulation mechanisms for the vegetative cell cycle and encystment of these ciliates are poorly understood. Here, transcriptomes of Apodileptus cf. visscheri, which has an asexual vegetative cell
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Expanding the molecular and morphological diversity of Apusomonadida, a deep-branching group of gliding bacterivorous protists J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-11-30 Guifré Torruella, Luis Javier Galindo, David Moreira, Maria Ciobanu, Aaron A. Heiss, Naoji Yubuki, Eunsoo Kim, Purificación López-García
Apusomonads are cosmopolitan bacterivorous biflagellate protists usually gliding on freshwater and marine sediment or wet soils. These nanoflagellates form a sister lineage to opisthokonts and may have retained ancestral features helpful to understanding the early evolution of this large supergroup. Although molecular environmental analyses indicate that apusomonads are genetically diverse, few species
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Pontisma blauvikense sp. nov. the first member of the early-diverging oomycete genus Pontisma parasitizing brown algae J. Eukaryot. Microbiol. (IF 2.2) Pub Date : 2022-11-29 Anthony Buaya, Ichen Tsai, Marco Thines
Holocarpic oomycetes have been neglected over several decades, until interest in these organisms has recently resurged. One of the most widespread genera of holocarpic oomycetes is Pontisma, parasitic to red seaweeds throughout all oceans. Recently, the genus Sirolpidium (parasitic to green algae) was found to be congeneric with Pontisma. This hinted at a high pathogenic versatility and prompted the