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  • The Nucleoid Occlusion Protein SlmA Binds to Lipid Membranes.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Miguel Ángel Robles-Ramos,William Margolin,Marta Sobrinos-Sanguino,Carlos Alfonso,Germán Rivas,Begoña Monterroso,Silvia Zorrilla

    Protection of the chromosome from scission by the division machinery during cytokinesis is critical for bacterial survival and fitness. This is achieved by nucleoid occlusion, which, in conjunction with other mechanisms, ensures formation of the division ring at midcell. In Escherichia coli, this mechanism is mediated by SlmA, a specific DNA binding protein that antagonizes assembly of the central

    更新日期:2020-10-28
  • Interspecies Microbial Fusion and Large-Scale Exchange of Cytoplasmic Proteins and RNA in a Syntrophic Clostridium Coculture.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Kamil Charubin,Shannon Modla,Jeffrey L Caplan,Eleftherios Terry Papoutsakis

    Microbial syntrophy is universal in nature, profoundly affecting the composition and function of microbiomes. We have recently reported data suggesting direct cell-to-cell interactions leading to electron and material exchange between the two microbes in the syntrophy between Clostridium ljungdahlii and C. acetobutylicum. Here, transmission electron microscopy and electron tomography demonstrated cell

    更新日期:2020-10-28
  • The ClpX and ClpP2 Orthologs of Chlamydia trachomatis Perform Discrete and Essential Functions in Organism Growth and Development.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Nicholas A Wood,Amanda M Blocker,Mohamed A Seleem,Martin Conda-Sheridan,Derek J Fisher,Scot P Ouellette

    Chlamydia trachomatis is an obligate intracellular bacterium that undergoes a complex developmental cycle in which the bacterium differentiates between two functionally and morphologically distinct forms, the elementary body (EB) and reticulate body (RB), each of which expresses its own specialized repertoire of proteins. Both primary (EB to RB) and secondary (RB to EB) differentiations require protein

    更新日期:2020-10-28
  • Molecular Deceleration Regulates Toxicant Release to Prevent Cell Damage in Pseudomonas putida S16 (DSM 28022).
    mBio (IF 6.784) Pub Date : 2020-09-01
    Hongzhi Tang,Kunzhi Zhang,Haiyang Hu,Geng Wu,Weiwei Wang,Xiongyu Zhu,Gongquan Liu,Ping Xu

    The underlying molecular mechanisms of flavin-dependent amine oxidases remain relatively poorly understood, even though many of these enzymes have been reported. The nicotine oxidoreductase NicA2 is a crucial enzyme for the first step of nicotine degradation in Pseudomonas putida S16 (DSM 28022). Here, we present the crystal structure of a ternary complex comprising NicA2 residues 21 to 482, flavin

    更新日期:2020-10-28
  • Aerobic Metabolism in Vibrio cholerae Is Required for Population Expansion during Infection.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Andrew J Van Alst,Victor J DiRita

    Vibrio cholerae replicates to high cell density in the human small intestine, leading to the diarrheal disease cholera. During infection, V. cholerae senses and responds to environmental signals that govern cellular responses. Spatial localization of V. cholerae within the intestine affects nutrient availability and metabolic pathways required for replicative success. Metabolic processes used by V

    更新日期:2020-10-28
  • HbtR, a Heterofunctional Homolog of the Virulence Regulator TcpP, Facilitates the Transition between Symbiotic and Planktonic Lifestyles in Vibrio fischeri.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Brittany D Bennett,Tara Essock-Burns,Edward G Ruby

    The bioluminescent bacterium Vibrio fischeri forms a mutually beneficial symbiosis with the Hawaiian bobtail squid, Euprymna scolopes, in which the bacteria, housed inside a specialized light organ, produce light used by the squid in its nocturnal activities. Upon hatching, E. scolopes juveniles acquire V. fischeri from the seawater through a complex process that requires, among other factors, chemotaxis

    更新日期:2020-10-28
  • A Novel Mycovirus Evokes Transcriptional Rewiring in the Fungus Malassezia and Stimulates Beta Interferon Production in Macrophages.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Shelly Applen Clancey,Fiorella Ruchti,Salomé LeibundGut-Landmann,Joseph Heitman,Giuseppe Ianiri

    Mycoviruses infect fungi, and while most persist asymptomatically, there are examples of mycoviruses having both beneficial and detrimental effects on their host. Virus-infected Saccharomyces and Ustilago strains exhibit a killer phenotype conferring a growth advantage over uninfected strains and other competing yeast species, whereas hypovirus-infected Cryphonectria parasitica displays defects in

    更新日期:2020-10-28
  • A Novel Virus Alters Gene Expression and Vacuolar Morphology in Malassezia Cells and Induces a TLR3-Mediated Inflammatory Immune Response.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Minji Park,Yong-Joon Cho,Donggyu Kim,Chul-Su Yang,Shi Mun Lee,Thomas L Dawson,Satoshi Nakamizo,Kenji Kabashima,Yang Won Lee,Won Hee Jung

    Most fungal viruses have been identified in plant pathogens, whereas the presence of viral particles in human-pathogenic fungi is less well studied. In the present study, we observed extrachromosomal double-stranded RNA (dsRNA) segments in various clinical isolates of Malassezia species. Malassezia is the most dominant fungal genus on the human skin surface, and species in this group are considered

    更新日期:2020-10-28
  • Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Paul D Veith,Mikio Shoji,Richard A J O'Hair,Michael G Leeming,Shuai Nie,Michelle D Glew,Gavin E Reid,Koji Nakayama,Eric C Reynolds

    Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids. In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure. Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K. The residual

    更新日期:2020-10-28
  • Structural Characterization of the Essential Cell Division Protein FtsE and Its Interaction with FtsX in Streptococcus pneumoniae.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Martin Alcorlo,Daniel Straume,Joe Lutkenhaus,Leiv Sigve Håvarstein,Juan A Hermoso

    FtsEX is a membrane complex widely conserved across diverse bacterial genera and involved in critical processes such as recruitment of division proteins and in spatial and temporal regulation of muralytic activity during cell division or sporulation. FtsEX is a member of the ABC transporter superfamily. The component FtsX is an integral membrane protein, whereas FtsE is an ATPase and is required for

    更新日期:2020-10-28
  • Unveiling Crucivirus Diversity by Mining Metagenomic Data.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Ignacio de la Higuera,George W Kasun,Ellis L Torrance,Alyssa A Pratt,Amberlee Maluenda,Jonathan Colombet,Maxime Bisseux,Viviane Ravet,Anisha Dayaram,Daisy Stainton,Simona Kraberger,Peyman Zawar-Reza,Sharyn Goldstien,James V Briskie,Robyn White,Helen Taylor,Christopher Gomez,David G Ainley,Jon S Harding,Rafaela S Fontenele,Joshua Schreck,Simone G Ribeiro,Stephen A Oswald,Jennifer M Arnold,François Enault

    The discovery of cruciviruses revealed the most explicit example of a common protein homologue between DNA and RNA viruses to date. Cruciviruses are a novel group of circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viruses that encode capsid proteins that are most closely related to those encoded by RNA viruses in the family Tombusviridae. The apparent chimeric nature of the two core proteins

    更新日期:2020-10-28
  • Histone Modifications and Other Facets of Epigenetic Regulation in Trypanosomatids: Leaving Their Mark.
    mBio (IF 6.784) Pub Date : 2020-09-01
    Swati Saha

    Histone posttranslational modifications (PTMs) modulate several eukaryotic cellular processes, including transcription, replication, and repair. Vast arrays of modifications have been identified in conventional eukaryotes over the last 20 to 25 years. While initial studies uncovered these primarily on histone tails, multiple modifications were subsequently found on the central globular domains as well

    更新日期:2020-10-28
  • An Early Pandemic Analysis of SARS-CoV-2 Population Structure and Dynamics in Arizona.
    mBio (IF 6.784) Pub Date : 2020-09-04
    Jason T Ladner,Brendan B Larsen,Jolene R Bowers,Crystal M Hepp,Evan Bolyen,Megan Folkerts,Krystal Sheridan,Ashlyn Pfeiffer,Hayley Yaglom,Darrin Lemmer,Jason W Sahl,Emily A Kaelin,Rabia Maqsood,Nicholas A Bokulich,Grace Quirk,Thomas D Watts,Kenneth K Komatsu,Victor Waddell,Efrem S Lim,J Gregory Caporaso,David M Engelthaler,Michael Worobey,Paul Keim

    In December of 2019, a novel coronavirus, SARS-CoV-2, emerged in the city of Wuhan, China, causing severe morbidity and mortality. Since then, the virus has swept across the globe, causing millions of confirmed infections and hundreds of thousands of deaths. To better understand the nature of the pandemic and the introduction and spread of the virus in Arizona, we sequenced viral genomes from clinical

    更新日期:2020-10-28
  • Network Rewiring: Physiological Consequences of Reciprocally Exchanging the Physical Locations and Growth-Phase-Dependent Expression Patterns of the Salmonella fis and dps Genes.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Marina M Bogue,Aalap Mogre,Michael C Beckett,Nicholas R Thomson,Charles J Dorman

    The Fis nucleoid-associated protein controls the expression of a large and diverse regulon of genes in Gram-negative bacteria. Fis production is normally maximal in bacteria during the early exponential phase of batch culture growth, becoming almost undetectable by the onset of stationary phase. We tested the effect on the Fis regulatory network in Salmonella of moving the complete fis gene from its

    更新日期:2020-10-28
  • Molecular Dialogues between Early Divergent Fungi and Bacteria in an Antagonism versus a Mutualism.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Olga A Lastovetsky,Lev D Krasnovsky,Xiaotian Qin,Maria L Gaspar,Andrii P Gryganskyi,Marcel Huntemann,Alicia Clum,Manoj Pillay,Krishnaveni Palaniappan,Neha Varghese,Natalia Mikhailova,Dimitrios Stamatis,T B K Reddy,Chris Daum,Nicole Shapiro,Natalia Ivanova,Nikos Kyrpides,Tanja Woyke,Teresa E Pawlowska

    Fungal-bacterial symbioses range from antagonisms to mutualisms and remain one of the least understood interdomain interactions despite their ubiquity as well as ecological and medical importance. To build a predictive conceptual framework for understanding interactions between fungi and bacteria in different types of symbioses, we surveyed fungal and bacterial transcriptional responses in the mutualism

    更新日期:2020-10-28
  • Laccase Affects the Rate of Cryptococcus neoformans Nonlytic Exocytosis from Macrophages.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Stefânia de Oliveira Frazão,Herdson Renney de Sousa,Lenise Gonçalves da Silva,Jéssica Dos Santos Folha,Kaio César de Melo Gorgonha,Getúlio Pereira de Oliveira,Maria Sueli Soares Felipe,Ildinete Silva-Pereira,Arturo Casadevall,André Moraes Nicola,Patrícia Albuquerque

    Nonlytic exocytosis is a process in which previously ingested microbes are expelled from host phagocytes with the concomitant survival of both cell types. This process has been observed in the interaction of Cryptococcus spp. and other fungal cells with phagocytes as distant as mammalian, bird, and fish macrophages and ameboid predators. Despite a great amount of research dedicated to unraveling this

    更新日期:2020-10-28
  • The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Michael P Motley,Elizabeth Diago-Navarro,Kasturi Banerjee,Sean Inzerillo,Bettina C Fries

    Monoclonal antibodies (MAbs) have the potential to assist in the battle against multidrug-resistant bacteria such as carbapenem-resistant Klebsiella pneumoniae (CR-Kp). However, the characteristics by which these antibodies (Abs) function, such as the role of antibody subclass, must be determined before such modalities can be carried from the bench to the bedside. We performed a subclass switch on

    更新日期:2020-10-28
  • Phase Transitions by an Abundant Protein in the Anammox Extracellular Matrix Mediate Cell-to-Cell Aggregation and Biofilm Formation.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Thomas Seviour,Lan Li Wong,Yang Lu,Sudarsan Mugunthan,Qiaohui Yang,Uma Shankari,Irina Bessarab,David Liebl,Rohan B H Williams,Yingyu Law,Staffan Kjelleberg

    This study describes the first direct functional assignment of a highly abundant extracellular protein from a key environmental and biotechnological biofilm performing an anaerobic ammonium oxidation (anammox) process. Expression levels of Brosi_A1236, belonging to a class of proteins previously suggested to be cell surface associated, were in the top one percentile of all genes in the “Candidatus

    更新日期:2020-10-28
  • Survey of Early-Diverging Lineages of Fungi Reveals Abundant and Diverse Mycoviruses.
    mBio (IF 6.784) Pub Date : 2020-09-08
    J M Myers,A E Bonds,R A Clemons,N A Thapa,D R Simmons,D Carter-House,J Ortanez,P Liu,A Miralles-Durán,A Desirò,J E Longcore,G Bonito,J E Stajich,J W Spatafora,Y Chang,L M Corrochano,A Gryganskyi,I V Grigoriev,T Y James

    Mycoviruses are widespread and purportedly common throughout the fungal kingdom, although most are known from hosts in the two most recently diverged phyla, Ascomycota and Basidiomycota, together called Dikarya. To augment our knowledge of mycovirus prevalence and diversity in underexplored fungi, we conducted a large-scale survey of fungi in the earlier-diverging lineages, using both culture-based

    更新日期:2020-10-28
  • Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Homer Pantua,Elizabeth Skippington,Marie-Gabrielle Braun,Cameron L Noland,Jingyu Diao,Yutian Peng,Susan L Gloor,Donghong Yan,Jing Kang,Anand Kumar Katakam,Janina Reeder,Georgette M Castanedo,Keira Garland,Laszlo Komuves,Meredith Sagolla,Cary D Austin,Jeremy Murray,Yiming Xu,Zora Modrusan,Min Xu,Emily J Hanan,Sharookh B Kapadia

    Clinical development of antibiotics with novel mechanisms of action to kill pathogenic bacteria is challenging, in part, due to the inevitable emergence of resistance. A phenomenon of potential clinical importance that is broadly overlooked in preclinical development is heteroresistance, an often-unstable phenotype in which subpopulations of bacterial cells show decreased antibiotic susceptibility

    更新日期:2020-10-28
  • Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Katelyn R Soules,Scott D LaBrie,Benjamin H May,P Scott Hefty

    Chlamydia bacteria are obligate intracellular organisms with a phylum-defining biphasic developmental cycle that is intrinsically linked to its ability to cause disease. The progression of the chlamydial developmental cycle is regulated by the temporal expression of genes predominantly controlled by RNA polymerase sigma (σ) factors. Sigma 54 (σ54) is one of three sigma factors encoded by Chlamydia

    更新日期:2020-10-28
  • Repetitive Elements Contribute to the Diversity and Evolution of Centromeres in the Fungal Genus Verticillium.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Michael F Seidl,H Martin Kramer,David E Cook,Gabriel L Fiorin,Grardy C M van den Berg,Luigi Faino,Bart P H J Thomma

    Centromeres are chromosomal regions that are crucial for chromosome segregation during mitosis and meiosis, and failed centromere formation can contribute to chromosomal anomalies. Despite this conserved function, centromeres differ significantly between and even within species. Thus far, systematic studies into the organization and evolution of fungal centromeres remain scarce. In this study, we identified

    更新日期:2020-10-28
  • Staphylococcus aureus ATP Synthase Promotes Biofilm Persistence by Influencing Innate Immunity.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Megan E Bosch,Blake P Bertrand,Cortney E Heim,Abdulelah A Alqarzaee,Sujata S Chaudhari,Amy L Aldrich,Paul D Fey,Vinai C Thomas,Tammy Kielian

    Staphylococcus aureus is a major cause of prosthetic joint infection (PJI), which is characterized by biofilm formation. S. aureus biofilm skews the host immune response toward an anti-inflammatory profile by the increased recruitment of myeloid-derived suppressor cells (MDSCs) that attenuate macrophage proinflammatory activity, leading to chronic infection. A screen of the Nebraska Transposon Mutant

    更新日期:2020-10-28
  • The Structure of the Cysteine-Rich Domain of Plasmodium falciparum P113 Identifies the Location of the RH5 Binding Site.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Ivan Campeotto,Francis Galaway,Shahid Mehmood,Lea K Barfod,Doris Quinkert,Vinayaka Kotraiah,Timothy W Phares,Katherine E Wright,Ambrosius P Snijders,Simon J Draper,Matthew K Higgins,Gavin J Wright

    Plasmodium falciparum RH5 is a secreted parasite ligand that is essential for erythrocyte invasion through direct interaction with the host erythrocyte receptor basigin. RH5 forms a tripartite complex with two other secreted parasite proteins, CyRPA and RIPR, and is tethered to the surface of the parasite through membrane-anchored P113. Antibodies against RH5, CyRPA, and RIPR can inhibit parasite invasion

    更新日期:2020-10-28
  • How the Anaerobic Enteropathogen Clostridioides difficile Tolerates Low O2 Tensions.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Nicolas Kint,Carolina Alves Feliciano,Maria C Martins,Claire Morvan,Susana F Fernandes,Filipe Folgosa,Bruno Dupuy,Miguel Texeira,Isabelle Martin-Verstraete

    Clostridioides difficile is a major cause of diarrhea associated with antibiotherapy. After germination of C. difficile spores in the small intestine, vegetative cells are exposed to low oxygen (O2) tensions. While considered strictly anaerobic, C. difficile is able to grow in nonstrict anaerobic conditions (1 to 3% O2) and tolerates brief air exposure indicating that this bacterium harbors an arsenal

    更新日期:2020-10-28
  • Live-Cell FRET Reveals that Malaria Nutrient Channel Proteins CLAG3 and RhopH2 Remain Associated throughout Their Tortuous Trafficking.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Moaz Ahmad,Javier Manzella-Lapeira,Gagandeep Saggu,Daisuke Ito,Joseph A Brzostowski,Sanjay A Desai

    Malaria parasites increase their host erythrocyte’s permeability to various nutrients, fueling intracellular pathogen development and replication. The plasmodial surface anion channel (PSAC) mediates this uptake and is linked to the parasite-encoded RhopH complex, consisting of CLAG3, RhopH2, and RhopH3. While interactions between these subunits are well established, it is not clear whether they remain

    更新日期:2020-10-28
  • Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
    mBio (IF 6.784) Pub Date : 2020-09-08
    Xiaoqian Yu,Thomas Gurry,Le Thanh Tu Nguyen,Hunter S Richardson,Eric J Alm

    Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs). While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body. Here

    更新日期:2020-10-28
  • Antiviral Activity of Type I, II, and III Interferons Counterbalances ACE2 Inducibility and Restricts SARS-CoV-2.
    mBio (IF 6.784) Pub Date : 2020-09-10
    Idoia Busnadiego,Sonja Fernbach,Marie O Pohl,Umut Karakus,Michael Huber,Alexandra Trkola,Silke Stertz,Benjamin G Hale

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is a recently emerged respiratory coronavirus that has infected >23 million people worldwide with >800,000 deaths. Few COVID-19 therapeutics are available, and the basis for severe infections is poorly understood. Here, we investigated properties of type I (β), II (γ), and III (λ1) interferons

    更新日期:2020-10-28
  • Evidence That VirS Is a Receptor for the Signaling Peptide of the Clostridium perfringens Agr-like Quorum Sensing System.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Jihong Li,Bruce A McClane

    Since both the Agr (accessory gene regulator)-like quorum sensing (QS) system and VirS/VirR (VirS/R) two-component regulatory system of Clostridium perfringens positively regulate production of several toxins, including C. perfringens beta toxin (CPB), it has been hypothesized the VirS membrane sensor protein is an Agr-like QS signaling peptide (SP) receptor. To begin evaluating whether VirS is an

    更新日期:2020-10-28
  • Bacteria Contribute to Plant Secondary Compound Degradation in a Generalist Herbivore System.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Charlotte B Francoeur,Lily Khadempour,Rolando D Moreira-Soto,Kirsten Gotting,Adam J Book,Adrián A Pinto-Tomás,Ken Keefover-Ring,Cameron R Currie

    Herbivores must overcome a variety of plant defenses, including coping with plant secondary compounds (PSCs). To help detoxify these defensive chemicals, several insect herbivores are known to harbor gut microbiota with the metabolic capacity to degrade PSCs. Leaf-cutter ants are generalist herbivores, obtaining sustenance from specialized fungus gardens that act as external digestive systems and which

    更新日期:2020-10-28
  • It's the Little Things (in Viral RNA).
    mBio (IF 6.784) Pub Date : 2020-09-15
    Jiří František Potužník,Hana Cahová

    Chemical modifications of viral RNA are an integral part of the viral life cycle and are present in most classes of viruses. To date, more than 170 RNA modifications have been discovered in all types of cellular RNA. Only a few, however, have been found in viral RNA, and the function of most of these has yet to be elucidated. Those few we have discovered and whose functions we understand have a varied

    更新日期:2020-10-28
  • Mechanistic Understanding Enables the Rational Design of Salicylanilide Combination Therapies for Gram-Negative Infections.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Janine N Copp,Daniel Pletzer,Alistair S Brown,Joris Van der Heijden,Charlotte M Miton,Rebecca J Edgar,Michelle H Rich,Rory F Little,Elsie M Williams,Robert E W Hancock,Nobuhiko Tokuriki,David F Ackerley

    One avenue to combat multidrug-resistant Gram-negative bacteria is the coadministration of multiple drugs (combination therapy), which can be particularly promising if drugs synergize. The identification of synergistic drug combinations, however, is challenging. Detailed understanding of antibiotic mechanisms can address this issue by facilitating the rational design of improved combination therapies

    更新日期:2020-10-28
  • MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Changqing Yu,Sunan Li,Xianfeng Zhang,Ilyas Khan,Iqbal Ahmad,Yulong Zhou,Shuo Li,Jing Shi,Yu Wang,Yong-Hui Zheng

    Membrane-associated RING-CH-type 8 (MARCH8) strongly blocks human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) incorporation into virions by downregulating its cell surface expression, but the mechanism is still unclear. We now report that MARCH8 also blocks the Ebola virus (EBOV) glycoprotein (GP) incorporation via surface downregulation. To understand how these viral fusion proteins

    更新日期:2020-10-28
  • A Potent Postentry Restriction to Primate Lentiviruses in a Yinpterochiropteran Bat.
    mBio (IF 6.784) Pub Date : 2020-09-15
    James H Morrison,Caitlin Miller,Laura Bankers,Gary Crameri,Lin-Fa Wang,Eric M Poeschla

    Bats are primary reservoirs for multiple lethal human viruses, such as Ebola, Nipah, Hendra, rabies, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome-related coronavirus (MERS-CoV), and, most recently, SARS-CoV-2. The innate immune systems of these immensely abundant, anciently diverged mammals remain insufficiently characterized. While bat genomes contain

    更新日期:2020-10-28
  • ExPortal and the LiaFSR Regulatory System Coordinate the Response to Cell Membrane Stress in Streptococcus pyogenes.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Yibin Lin,Misu A Sanson,Luis Alberto Vega,Brittany Shah,Shrijana Regmi,M Belen Cubria,Anthony R Flores

    LiaFSR is a gene regulatory system important for response to cell membrane stress in Gram-positive bacteria but is minimally studied in the important human pathogen group A Streptococcus (GAS). Using immunofluorescence and immunogold electron microscopy, we discovered that LiaF (a membrane-bound repressor protein) and LiaS (a sensor kinase) reside within the GAS membrane microdomain (ExPortal). Cell

    更新日期:2020-10-28
  • Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Jian Chen,Zhilu Chen,Mingbin Liu,Tianyi Qiu,Daobin Feng,Chen Zhao,Shuye Zhang,Xiaoyan Zhang,Jianqing Xu

    Zika virus (ZIKV) infection during pregnancy causes intrauterine growth defects and microcephaly, but knowledge of the mechanism through which ZIKV infects and replicates in the placenta remains elusive. Here, we found that ALPP, an alkaline phosphatase expressed primarily in placental tissue, promoted ZIKV infection in both human placental trophoblasts and astrocytoma cells. ALPP bound to ZIKV structural

    更新日期:2020-10-28
  • Mitochondrial Targeting of the Enteropathogenic Escherichia coli Map Triggers Calcium Mobilization, ADAM10-MAP Kinase Signaling, and Host Cell Apoptosis.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Rachana Pattani Ramachandran,Chaya Spiegel,Yael Keren,Tsafi Danieli,Naomi Melamed-Book,Ritesh Ranjan Pal,Efrat Zlotkin-Rivkin,Ilan Rosenshine,Benjamin Aroeti

    The ability of diarrheagenic bacterial pathogens, such as enteropathogenic Escherichia coli (EPEC), to modulate the activity of mitogen-activated protein kinases (MAPKs) and cell survival has been suggested to benefit bacterial colonization and infection. However, our understanding of the mechanisms by which EPEC modulate these functions is incomplete. In this study, we show that the EPEC type III

    更新日期:2020-10-28
  • Offloading Role of a Discrete Thioesterase in Type II Polyketide Biosynthesis.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Kangmin Hua,Xiangyang Liu,Yuchun Zhao,Yaojie Gao,Lifeng Pan,Haoran Zhang,Zixin Deng,Ming Jiang

    Type II polyketides are a group of secondary metabolites with various biological activities. In nature, biosynthesis of type II polyketides involves multiple enzymatic steps whereby key enzymes, including ketoacyl-synthase (KSα), chain length factor (KSβ), and acyl carrier protein (ACP), are utilized to elongate the polyketide chain through a repetitive condensation reaction. During each condensation

    更新日期:2020-10-28
  • The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Alejandro Tejada-Arranz,Eloïse Galtier,Lamya El Mortaji,Evelyne Turlin,Dmitry Ershov,Hilde De Reuse

    Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these organelleless cells. The minimal RNA degradosome of the important gastric pathogen Helicobacter pylori is composed of the essential ribonuclease RNase

    更新日期:2020-10-28
  • CD4+ T Cell-Mimicking Nanoparticles Broadly Neutralize HIV-1 and Suppress Viral Replication through Autophagy.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Gang Zhang,Grant R Campbell,Qiangzhe Zhang,Erin Maule,Jonathan Hanna,Weiwei Gao,Liangfang Zhang,Stephen A Spector

    Therapeutic strategies that provide effective and broad‐spectrum neutralization against HIV-1 infection are highly desirable. Here, we investigate the potential of nanoengineered CD4+ T cell membrane-coated nanoparticles (TNP) to neutralize a broad range of HIV-1 strains. TNP displayed outstanding neutralizing breadth and potency; they neutralized all 125 HIV-1-pseudotyped viruses tested, including

    更新日期:2020-10-28
  • CsrA-Mediated Translational Activation of ymdA Expression in Escherichia coli.
    mBio (IF 6.784) Pub Date : 2020-09-15
    Andrew Renda,Stephanie Poly,Ying-Jung Lai,Archana Pannuri,Helen Yakhnin,Anastasia H Potts,Philip C Bevilacqua,Tony Romeo,Paul Babitzke

    The sequence-specific RNA-binding protein CsrA is the central component of the conserved global regulatory Csr system. In Escherichia coli, CsrA regulates many cellular processes, including biofilm formation, motility, carbon metabolism, iron homeostasis, and stress responses. Such regulation often involves translational repression by CsrA binding to an mRNA target, thereby inhibiting ribosome binding

    更新日期:2020-10-28
  • Impaired Cytotoxic CD8+ T Cell Response in Elderly COVID-19 Patients.
    mBio (IF 6.784) Pub Date : 2020-09-18
    Jaana Westmeier,Krystallenia Paniskaki,Zehra Karaköse,Tanja Werner,Kathrin Sutter,Sebastian Dolff,Marvin Overbeck,Andreas Limmer,Jia Liu,Xin Zheng,Thorsten Brenner,Marc M Berger,Oliver Witzke,Mirko Trilling,Mengji Lu,Dongliang Yang,Nina Babel,Timm Westhoff,Ulf Dittmer,Gennadiy Zelinskyy

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces a T cell response that most likely contributes to virus control in COVID-19 patients but may also induce immunopathology. Until now, the cytotoxic T cell response has not been very well characterized in COVID-19 patients. Here, we analyzed the differentiation and cytotoxic profile of T cells in 30 cases of mild COVID-19

    更新日期:2020-10-28
  • Biophysical and Biochemical Characterization of TP0037, a d-Lactate Dehydrogenase, Supports an Acetogenic Energy Conservation Pathway in Treponema pallidum.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Ranjit K Deka,Wei Z Liu,Michael V Norgard,Chad A Brautigam

    A longstanding conundrum in Treponema pallidum biology concerns how the spirochete generates sufficient energy to fulfill its complex pathogenesis processes during human syphilitic infection. For decades, it has been assumed that the bacterium relies solely on glucose catabolism (via glycolysis) for generation of its ATP. However, the organism’s robust motility, believed to be essential for human tissue

    更新日期:2020-10-28
  • Intergenerational Pathogen-Induced Diapause in Caenorhabditis elegans Is Modulated by mir-243.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Carolaing Gabaldón,Marcela Legüe,M Fernanda Palominos,Lidia Verdugo,Florence Gutzwiller,Andrea Calixto

    The interaction and communication between bacteria and their hosts modulate many aspects of animal physiology and behavior. Dauer entry as a response to chronic exposure to pathogenic bacteria in Caenorhabditis elegans is an example of a dramatic survival response. This response is dependent on the RNA interference (RNAi) machinery, suggesting the involvement of small RNAs (sRNAs) as effectors. Interestingly

    更新日期:2020-10-28
  • Genotoxic Effect of Salmonella Paratyphi A Infection on Human Primary Gallbladder Cells.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Ludovico P Sepe,Kimberly Hartl,Amina Iftekhar,Hilmar Berger,Naveen Kumar,Christian Goosmann,Sascha Chopra,Sven Christian Schmidt,Rajendra Kumar Gurumurthy,Thomas F Meyer,Francesco Boccellato

    Carcinoma of the gallbladder (GBC) is the most frequent tumor of the biliary tract. Despite epidemiological studies showing a correlation between chronic infection with Salmonella enterica Typhi/Paratyphi A and GBC, the underlying molecular mechanisms of this fatal connection are still uncertain. The murine serovar Salmonella Typhimurium has been shown to promote transformation of genetically predisposed

    更新日期:2020-10-28
  • Engineering of Long-Circulating Peptidoglycan Hydrolases Enables Efficient Treatment of Systemic Staphylococcus aureus Infection.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Anna M Sobieraj,Markus Huemer,Léa V Zinsli,Susanne Meile,Anja P Keller,Christian Röhrig,Fritz Eichenseher,Yang Shen,Annelies S Zinkernagel,Martin J Loessner,Mathias Schmelcher

    Staphylococcus aureus is a human pathogen causing life-threatening diseases. The increasing prevalence of multidrug-resistant S. aureus infections is a global health concern, requiring development of novel therapeutic options. Peptidoglycan-degrading enzymes (peptidoglycan hydrolases, PGHs) have emerged as a highly effective class of antimicrobial proteins against S. aureus and other pathogens. When

    更新日期:2020-10-28
  • The Small Protein RmpD Drives Hypermucoviscosity in Klebsiella pneumoniae.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Kimberly A Walker,Logan P Treat,Victoria E Sepúlveda,Virginia L Miller

    Klebsiella pneumoniae has a remarkable ability to cause a wide range of human diseases. It is divided into two broad classes: classical strains that are a notable problem in health care settings due to multidrug resistance, and hypervirulent (hv) strains that are historically drug sensitive but able to establish disease in immunocompetent hosts. Alarmingly, there has been an increased frequency of

    更新日期:2020-10-28
  • Outer Membrane Disruption Overcomes Intrinsic, Acquired, and Spontaneous Antibiotic Resistance.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Craig R MacNair,Eric D Brown

    Disruption of the outer membrane (OM) barrier allows for the entry of otherwise inactive antimicrobials into Gram-negative pathogens. Numerous efforts to implement this approach have identified a large number of OM perturbants that sensitize Gram-negative bacteria to many clinically available Gram-positive active antibiotics. However, there is a dearth of investigation into the strengths and limitations

    更新日期:2020-10-28
  • Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax).
    mBio (IF 6.784) Pub Date : 2020-09-22
    Morten Simonsen Dueholm,Kasper Skytte Andersen,Simon Jon McIlroy,Jannie Munk Kristensen,Erika Yashiro,Søren Michael Karst,Mads Albertsen,Per Halkjær Nielsen

    High-throughput 16S rRNA gene amplicon sequencing is an essential method for studying the diversity and dynamics of microbial communities. However, this method is presently hampered by the lack of high-identity reference sequences for many environmental microbes in the public 16S rRNA gene reference databases and by the absence of a systematic and comprehensive taxonomy for the uncultured majority

    更新日期:2020-10-28
  • Minimal Elements Required for the Formation of Respiratory Syncytial Virus Cytoplasmic Inclusion Bodies In Vivo and In Vitro.
    mBio (IF 6.784) Pub Date : 2020-09-22
    Marie Galloux,Jennifer Risso-Ballester,Charles-Adrien Richard,Jenna Fix,Marie-Anne Rameix-Welti,Jean-François Eléouët

    Infection of host cells by the respiratory syncytial virus (RSV) is characterized by the formation of spherical cytoplasmic inclusion bodies (IBs). These structures, which concentrate all the proteins of the polymerase complex as well as some cellular proteins, were initially considered aggresomes formed by viral dead-end products. However, recent studies revealed that IBs are viral factories where

    更新日期:2020-10-28
  • Rescue of SARS-CoV-2 from a Single Bacterial Artificial Chromosome
    mBio (IF 6.784) Pub Date : 2020-09-25
    Chengjin Ye, Kevin Chiem, Jun-Gyu Park, Fatai Oladunni, Roy Nelson Platt, Tim Anderson, Fernando Almazan, Juan Carlos de la Torre, Luis Martinez-Sobrido, Anne Moscona

    Infectious coronavirus (CoV) disease 2019 (COVID-19) emerged in the city of Wuhan (China) in December 2019, causing a pandemic that has dramatically impacted public health and socioeconomic activities worldwide. A previously unknown coronavirus, severe acute respiratory syndrome CoV-2 (SARS-CoV-2), has been identified as the causative agent of COVID-19. To date, there are no U.S. Food and Drug Administration

    更新日期:2020-10-28
  • Antiviral Drug Discovery To Address the COVID-19 Pandemic
    mBio (IF 6.784) Pub Date : 2020-09-25
    Douglas D. Richman, Michael S. Diamond

    The magnitude of the morbidity and mortality inflicted upon the global population in less than 1 year has driven the inescapable conclusion that the discovery and development of effective antiviral drugs for COVID-19 are urgent and should be prioritized. The antiviral drug discovery programs that emerged for HIV and hepatitis C virus have enabled technology and expertise to accelerate this process

    更新日期:2020-10-28
  • S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit
    mBio (IF 6.784) Pub Date : 2020-09-25
    Phuong Nguyen-Contant, A. Karim Embong, Preshetha Kanagaiah, Francisco A. Chaves, Hongmei Yang, Angela R. Branche, David J. Topham, Mark Y. Sangster, Ali Ellebedy, Stacey Schultz-Cherry

    The high susceptibility of humans to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the cause of coronavirus disease 2019 (COVID-19), reflects the novelty of the virus and limited preexisting B cell immunity. IgG against the SARS-CoV-2 spike (S) protein, which carries the novel receptor binding domain (RBD), is absent or at low levels in unexposed individuals. To better understand

    更新日期:2020-10-28
  • SARS-CoV-2 Seroprevalence among a Southern U.S. Population Indicates Limited Asymptomatic Spread under Physical Distancing Measures
    mBio (IF 6.784) Pub Date : 2020-09-29
    Amir Barzin, John L. Schmitz, Samuel Rosin, Rameet Sirpal, Martha Almond, Carole Robinette, Samantha Wells, Michael Hudgens, Andrew Olshan, Stephanie Deen, Patrick Krejci, Eugenia Quackenbush, Kevin Chronowski, Caleb Cornaby, Janette Goins, Linda Butler, Julia Aucoin, Kim Boyer, Janet Faulk, Devena Alston-Johnson, Cristen Page, Yijun Zhou, Lynne Fiscus, Blossom Damania, Dirk P. Dittmer, David B. Peden

    Characterizing the asymptomatic spread of SARS-CoV-2 is important for understanding the COVID-19 pandemic. This study was aimed at determining asymptomatic spread of SARS-CoV-2 in a suburban, Southern U.S. population during a period of state restrictions and physical distancing mandates. This is one of the first published seroprevalence studies from North Carolina and included multicenter, primary

    更新日期:2020-10-28
  • Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain
    mBio (IF 6.784) Pub Date : 2020-09-29
    Shelby M. Lyon, Kristen D. Yetming, Christina Paulus, Michael Nevels, Robert F. Kalejta, Stacey Schultz-Cherry

    The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD). Here, we report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein. Deletion of the IE19 CTD or disruption of the IE19 splice acceptor site reduced viral

    更新日期:2020-10-28
  • A New Subclass of Exoribonuclease-Resistant RNA Found in Multiple Genera of Flaviviridae
    mBio (IF 6.784) Pub Date : 2020-09-29
    Matthew J. Szucs, Parker J. Nichols, Rachel A. Jones, Quentin Vicens, Jeffrey S. Kieft, Michael S. Diamond

    Viruses have developed innovative strategies to exploit the cellular machinery and overcome the antiviral defenses of the host, often using specifically structured RNA elements. Examples are found in the Flavivirus genus (in the family Flaviviridae), where during flaviviral infection, pathogenic subgenomic flaviviral RNAs (sfRNAs) accumulate in the cell. These sfRNAs are formed when a host cell 5′

    更新日期:2020-10-28
  • A Key Motif in the Cholesterol-Dependent Cytolysins Reveals a Large Family of Related Proteins
    mBio (IF 6.784) Pub Date : 2020-09-29
    Jordan C. Evans, Bronte A. Johnstone, Sara L. Lawrence, Craig J. Morton, Michelle P. Christie, Michael W. Parker, Rodney K. Tweten, Bruce A. McClane

    The cholesterol-dependent cytolysins (CDCs) are bacterial, β-barrel, pore-forming toxins. A central enigma of the pore-forming mechanism is how completion of the prepore is sensed to initiate its conversion to the pore. We identified a motif that is conserved between the CDCs and a diverse family of nearly 300 uncharacterized proteins present in over 220 species that span at least 10 bacterial and

    更新日期:2020-10-28
  • Adaptation of Arginine Synthesis among Uropathogenic Branches of the Escherichia coli Phylogeny Reveals Adjustment to the Urinary Tract Habitat
    mBio (IF 6.784) Pub Date : 2020-09-29
    Michael E. Hibbing, Karen W. Dodson, Vasilios Kalas, Swaine L. Chen, Scott J. Hultgren, Sheryl Justice

    Urinary tract infections (UTIs) are predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC pathogenesis requires passage through a severe population bottleneck involving intracellular bacterial communities (IBCs) that are clonal expansions of a single invading UPEC bacterium in a urothelial superficial facet cell. IBCs occur only during acute pathogenesis. The bacteria in IBCs form the

    更新日期:2020-10-28
  • Interleukin-1{beta} Triggers p53-Mediated Downmodulation of CCR5 and HIV-1 Entry in Macrophages through MicroRNAs 103 and 107
    mBio (IF 6.784) Pub Date : 2020-09-29
    Robert Lodge, Nicolas Bellini, Mélanie Laporte, Syim Salahuddin, Jean-Pierre Routy, Petronela Ancuta, Cecilia T. Costiniuk, Mohammad-Ali Jenabian, Éric A. Cohen, Monica J. Roth