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Exploring G protein-coupled receptors and yeast surface display strategies for viral detection in baker's yeast: SARS-CoV-2 as a case study FEMS Yeast Res. (IF 3.193) Pub Date : 2021-01-19 Carla Maneira; Pamela Magalí Bermejo; Gonçalo Amarante Guimarães Pereira; Fellipe da Silveira Bezerra de Mello
Viral infections pose intense burdens to healthcare systems and global economies. The correct diagnosis of viral diseases represents a crucial step towards effective treatments and control. Biosensors have been successfully implemented as accessible and accurate detection tests for some of the most important viruses. While most biosensors are based on physical or chemical interactions of cell-free
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Ethanol production process driving changes on industrial strains FEMS Yeast Res. (IF 3.193) Pub Date : 2021-01-08 Sheila Tiemi Nagamatsu; Natalia Coutouné; Juliana José; Mateus Bernabe Fiamenghi; Gonçalo Amarante Guimarães Pereira; Juliana Velasco de Castro Oliveira; Marcelo Falsarella Carazzolle
Ethanol production has key differences between the two largest producing countries of this biofuel, Brazil and the USA, such as feedstock source, sugar concentration and ethanol titers in industrial fermentation. Therefore, it is highly probable that these specificities have led to genome adaptation of the Saccharomyces cerevisiae strains employed in each process to tolerate different environments
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Physical, genetic and functional interactions between the eisosome protein Pil1 and the MBOAT O-acyltransferase Gup1 FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-23 Joana Tulha; Mariana Amorim-Rodrigues; Lidia Alejo Esquembre; Sebastien Rauch; Markus J Tamás; Cândida Lucas
The Saccharomyces cerevisiae MBOAT O-acyltransferase Gup1 is involved in many processes, including cell wall and membrane composition and integrity, and acetic acid-induced cell death. Gup1 was previously shown to interact physically with the mitochondrial membrane VDAC (Voltage-Dependent Anion Channel) protein Por1 and the ammonium transceptor Mep2. By co-immunoprecipitation, the eisosome core component
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A label-free real-time method for measuring glucose uptake kinetics in yeast FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-18 Schmidl S, Iancu C, Reifenrath M, et al.
ABSTRACTGlucose uptake assays commonly rely on the isotope-labeled sugar, which is associated with radioactive waste and exposure of the experimenter to radiation. Here, we show that the rapid decrease of the cytosolic pH after a glucose pulse to starved Saccharomyces cerevisiae cells is dependent on the rate of sugar uptake and can be used to determine the kinetic parameters of sugar transporters
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Metabolic engineering of Saccharomyces cerevisiae for production of β-carotene from hydrophobic substrates FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-17 Fathi Z, Tramontin L, Ebrahimipour G, et al.
ABSTRACTβ-Carotene is a yellow–orange–red pigment used in food, cosmetics and pharmacy. There is no commercial yeast-based process for β-carotene manufacturing. In this work, we engineered the baker's yeast Saccharomyces cerevisiae by expression of lipases and carotenogenic genes to enable the production of β-carotene on hydrophobic substrates. First, the extracellular lipase (LIP2) and two cell-bound
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D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain FEMS Yeast Res. (IF 3.193) Pub Date : 2020-11-24 Jeroen G Nijland; Hyun Yong Shin; Eleonora Dore; Donny Rudinatha; Paul P de Waal; Arnold J M Driessen
Co-consumption of D-xylose and D-glucose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. There is a need for improved sugar conversion rates to minimize fermentation times. Previously, we have employed evolutionary engineering to enhance D-xylose transport and metabolism in the presence of D-glucose in a xylose-fermenting S. cerevisiae strain devoid of
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Entering GATTACA: yeast genomes: analysis, insights and applications FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-17 Daran J.
A quarter of a century ago, publication of the 12 Mb genome sequence of Saccharomyces cerevisiae S288C transformed yeast research forever and further cemented the strength and status of this yeast as a versatile laboratory model for eukaryotic cells. Knowledge on the gene complement and genome sequence of S. cerevisiae inspired yeast scientists to address the next challenge: elucidation of the biological
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Evaluating accessibility, usability and interoperability of genome-scale metabolic models for diverse yeasts species FEMS Yeast Res. (IF 3.193) Pub Date : 2021-01-11 Iván Domenzain; Feiran Li; Eduard J Kerkhoven; Verena Siewers
Metabolic network reconstructions have become an important tool for probing cellular metabolism in the field of systems biology. They are used as tools for quantitative prediction but also as scaffolds for further knowledge contextualization. The yeast Saccharomyces cerevisiae was one of the first organisms for which a genome-scale metabolic model (GEM) was reconstructed, in 2003, and since then 45
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The non-Saccharomyces yeast Pichia kluyveri for the production of aromatic volatile compounds in alcoholic fermentation FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-14 Méndez-Zamora A, Gutiérrez-Avendaño D, Arellano-Plaza M, et al.
ABSTRACTAlcoholic fermentation is influenced by yeast strain, culture media, substrate concentration and fermentation conditions, which contribute to taste and aroma. Some non-Saccharomyces yeasts are recognized as volatile compound producers that enrich aromatic profile of alcoholic beverages. In this work, 21 strains of Pichia kluyveri isolated from different fermentative processes and regions were
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A TRP1-marker-based system for gene complementation, overexpression, reporter gene expression and gene modification in Candida glabrata FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-08 Sprenger M, Brunke S, Hube B, et al.
ABSTRACTAlthough less prevalent than its relative Candida albicans, the yeast Candida glabrata is a successful pathogen of humans, which causes life-threatening candidiasis. It is thus vital to understand the pathogenicity mechanisms and contributing genes in C. glabrata. However, gene complementation as a tool for restoring the function of a previously deleted gene is not standardized in C. glabrata
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From baker's yeast to genetically modified budding yeasts: the scientific evolution of bioethanol industry from sugarcane FEMS Yeast Res. (IF 3.193) Pub Date : 2020-12-04 Ceccato-Antonini S, Covre E.
ABSTRACTThe peculiarities of Brazilian fuel ethanol fermentation allow the entry of native yeasts that may dominate over the starter strains of Saccharomyces cerevisiae and persist throughout the sugarcane harvest. The switch from the use of baker's yeast as starter to selected budding yeasts obtained by a selective pressure strategy was followed by a wealth of genomic information that enabled the
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The native acyltransferase-coding genes DGA1 and DGA2 affect lipid accumulation in Blastobotrys raffinosifermentans differently when overexpressed FEMS Yeast Res. (IF 3.193) Pub Date : 2020-11-18 Sanya D, Onesime D, Kunze G, et al.
ABSTRACTBlastobotrys raffinosifermentans is an ascomycetous yeast with biotechnological applications, recently shown to be an oleaginous yeast accumulating lipids under nitrogen limitation. Diacylglycerol acyltransferases (DGATs) act in the lipid storage pathway, in the last step of triacylglycerol biosynthesis. Two DGAT families are widespread in eukaryotes. We first checked that B. raffinosifermentans
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Identification of the citrate exporter Cex1 of Yarrowia lipolytica FEMS Yeast Res. (IF 3.193) Pub Date : 2020-09-29 Anna Maria Erian; Michael Egermeier; Alice Rassinger; Hans Marx; Michael Sauer
Yarrowia lipolytica is a yeast with many talents, one of them being the production of citric acid. Although the citrate biosynthesis is well studied, little is known about the transport mechanism by which citrate is exported. To gain better insight into this mechanism, we set out to identify a transporter involved in citrate export of Y. lipolytica. A total of five proteins were selected for analysis
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The transcriptomic response of a wine strain of Lachancea thermotolerans to oxygen deprivation. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-09-22 Kirti Shekhawat,Florian F Bauer,Mathabatha E Setati
The yeast Lachancea thermotolerans is of significant biotechnological interest, and selected strains of this species have become commonly used starter cultures in wine fermentation. However, the impact of this species on wine is frequently limited by the rapid dominance of Saccharomyces cerevisiae strains which are better adapted to wine alcoholic fermentation conditions. Previous studies have shown
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Cell death in Ustilago maydis: comparison with other fungi and the effect of metformin and curcumin on its chronological lifespan. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-09-18 Cinthia V Soberanes-Gutiérrez,Claudia León-Ramírez,Lino Sánchez-Segura,Emmanuel Cordero-Martínez,Julio C Vega-Arreguín,José Ruiz-Herrera
Ustilago maydis is a Basidiomycota fungus, in which very little is known about its mechanisms of cell survival and death. To date, only the role of metacaspase1, acetate and hydrogen peroxide as inducers of cell death has been investigated.
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The unfolded protein response in Pichia pastoris without external stressing stimuli. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-09-14 Yasmin Nabilah Binti Mohd Fauzee,Naoki Taniguchi,Yuki Ishiwata-Kimata,Hiroshi Takagi,Yukio Kimata
Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which is translated into a transcription factor protein that is responsible for the transcriptome
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Comparative genome analysis proposes three new Aureobasidium species isolated from grape juice. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-09-08 Cristobal A Onetto,Simon A Schmidt,Michael J Roach,Anthony R Borneman
Aureobasidium pullulans is the most abundant and ubiquitous species within the genus and is also considered a core component of the grape juice microflora. So far, a small number of other Aureobasidium species have been reported, that in contrast to A. pullulans, appear far more constrained to specific habitats. It is unknown whether grape juice is a reservoir of novel Aureobasidium species, overlooked
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Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic hydrolysate. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-25 Feng Yao,Shun-Cheng Liu,Dan-Ni Wang,Zhi-Jie Liu,Qiang Hua,Liu-Jing Wei
Limonene, a valuable cyclic monoterpene, has been broadly studied in recent decades due to its wide application in the food, cosmetics and pharmaceutical industries. Engineering of the yeast Yarrowia lipolytica for fermentation of renewable biomass lignocellulosic hydrolysate may reduce the cost and improve the economics of bioconversion for the production of limonene. The aim of this study was to
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The inositol polyphosphate kinase Ipk1 transcriptionally regulates mitochondrial functions in Candida albicans. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-24 Hangqi Zhu,Nali Zhu,Liping Peng,Bing Zhang,Qilin Yu,Mingchun Li
Inositol polyphosphates (IPs) is an important family of signaling molecules that regulate multiple cellular processes, such as chromatin remodeling, transcription and mRNA export. Inositol polyphosphate kinases, as the critical enzymes for production and transformation of IPs, directly determine the intracellular levels of IPs and therefore are involved in many cellular processes. However, its roles
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The complete mitochondrial genome of the lipid-producing yeast Rhodotorula toruloides. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-13 Renhui Zhou,Zhiwei Zhu,Sufang Zhang,Zongbao Kent Zhao
Mitochondria are semi-autonomous organelles with their own genome and crucial to cellular material and energy metabolism. Here, we report the complete mitochondrial genome of a lipid-producing basidiomycetous yeast Rhodotorula toruloides NP11. The mitochondrial genome of R. toruloides NP11 was assembled into a circular DNA molecule of 125937bp, encoding 15 proteins, 28 transfer RNAs, 2 ribosomal RNA
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Towards yeast taxogenomics: lessons from novel species descriptions based on complete genome sequences. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-07-25 D Libkind,N Čadež,D A Opulente,Q K Langdon,C A Rosa,J P Sampaio,P Gonçalves,C T Hittinger,M A Lachance
In recent years, ‘multi-omic’ sciences have affected all aspects of fundamental and applied biological research. Yeast taxonomists, though somewhat timidly, have begun to incorporate complete genomic sequences into the description of novel taxa, taking advantage of these powerful data to calculate more reliable genetic distances, construct more robust phylogenies, correlate genotype with phenotype
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Editorial: yeast synthetic biology. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-27 Jin Hou,Jens Nielsen
Yeasts have long been used to produce alcoholic beverages and fuel ethanol. Its fast growth, well-developed genetics, robustness in large-scale fermentations and resistance to inhibitors and phages have made it the preferred microbial cell factories for production a broad range of valuable products such as biofuels, biochemicals, nutraceuticals and pharmaceuticals. Despite the successful applications
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Evaluating and engineering Saccharomyces cerevisiae promoters for increased amylase expression and bioethanol production from raw starch. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-12 Marthinus W Myburgh,Shaunita H Rose,Marinda Viljoen-Bloom
Bioethanol production from starchy biomass via consolidated bioprocessing (CBP) will benefit from amylolytic Saccharomyces cerevisiae strains that produce high levels of recombinant amylases. This could be achieved by using strong promoters and modification thereof to improve gene expression under industrial conditions. This study evaluated eight endogenous S. cerevisiae promoters for the expression
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A glimpse of potential transposable element impact on adaptation of the industrial yeast Saccharomyces cerevisiae. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-11 Z Lewis Liu,Xiaoqiu Huang
The adapted industrial yeast strain Saccharomyces cerevisiae NRRL Y-50049 is able to in situ detoxify major toxic aldehyde compounds derived from sugar conversion of lignocellulosic biomass while producing ethanol. Pathway-based studies on its mechanisms of tolerance have been reported previously, however, little is known about transposable element (TE) involvement in its adaptation to inhibitory compounds
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A detailed lipidomic study of human pathogenic fungi Candida auris. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-05 Garima Shahi,Mohit Kumar,Sonam Kumari,Shivaprakash M Rudramurthy,Arunaloke Chakrabarti,Naseem A Gaur,Ashutosh Singh,Rajendra Prasad
The present study is an attempt to determine the lipid composition of Candida auris and to highlight if the changes in lipids can be correlated to high drug resistance encountered in C. auris. For this, the comparative lipidomics landscape between drug-susceptible (CBS10913T) and a resistant hospital isolate (NCCPF_470033) of C. auris was determined by employing high throughput mass spectrometry. All
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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-08-07 Sonakshi De,Corinna Rebnegger,Josef Moser,Nadine Tatto,Alexandra B Graf,Diethard Mattanovich,Brigitte Gasser
ABSTRACTMany yeasts differentiate into multicellular phenotypes in adverse environmental conditions. Here, we investigate pseudohyphal growth in Komagataella phaffii and the involvement of the flocculin (FLO) gene family in its regulation. The K. phaffii FLO family consists of 13 members, and the conditions inducing pseudohyphal growth are different from Saccharomyces cerevisiae. So far, this phenotype
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Hybridization and the origin of new yeast lineages. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-07-13 Toni Gabaldón
Hybrids originate from the mating of two diverged organisms, resulting in novel lineages that have chimeric genomes. Hybrids may exhibit unique phenotypic traits that are not necessarily intermediate between those present in the progenitors. These unique traits may enable them to thrive in new environments. Many hybrid lineages have been discovered among yeasts in the Saccharomycotina, of which many
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Real-time detection of changes in yeast plasma membrane potential using genetically encoded voltage indicator proteins. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-07-21 Walrati Limapichat,Wichai Pornthanakasem,Chatchaya Satitthammachart,Penchit Chitnumsub,Ubolsree Leartsakulpanich
In yeast, adaptation to varying conditions often requires proper regulation of the plasma membrane potential. To determine yeast membrane potential change, optical methods involving potentiometric dyes have been supplemental to the direct electrode-based method. However, the hydrophobic nature of the dyes and their slow distribution across the membrane still limits their utilization. Genetically encoded
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Impairment of MET transcriptional activators, MET4 and MET31 induced lipid accumulation in Saccharomyces cerevisiae. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-07-10 Selvaraj Rajakumar,Vennila Suriyagandhi,Vasanthi Nachiappan
The genes involved in the methionine pathway are closely associated with phospholipid homeostasis in yeast. The impact of the deletion of methionine (MET) transcriptional activators (MET31, MET32 and MET4) in lipid homeostasis is studied. Our lipid profiling data showed that aberrant phospholipid and neutral lipid accumulation occurred in met31∆ and met4∆ strains with low Met. The expression pattern
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Water soluble lipid precursor contaminants in yeast culture medium ingredients. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-27 Mike F Renne,Xue Bao,Anton I P M de Kroon
The presence of the water soluble glycerophospholipid precursors choline and inositol in culture media highly affects lipid biosynthesis and regulation thereof. We report that widely used media ingredients contain trace amounts of choline and inositol that are not mentioned on the product label, influencing experimental outcome.
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Candida albicans Mrv8, is involved in epithelial damage and biofilm formation. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-25 Anna Carolina Borges Pereira Costa,Graziella Nuernberg Back-Brito,François L Mayer,Bernhard Hube,Duncan Wilson
Candida albicans is the most common human fungal pathogen that can cause superficial and deep-seated infections in susceptible individuals. Despite its medical importance, the vast majority of C. albicans genes remain of unknown function. Here, we report a role for the lineage-specific gene, MRV8, in host pathogen interactions, mycelial microcolony maturation and biofilm formation. In silico analysis
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Transcriptomic analysis reveals MAPK signaling pathways affect the autolysis in baker's yeast. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-18 Xiao Li,Han Ye,Chao-Qun Xu,Xiang-Ling Shen,Xiao-Long Zhang,Cong Huang,Ben Cheng,Ya-Li Tan,Ze-Tao Xiao,Yu-Peng Pei,Kun Zou
Yeast autolysis refers to the process in which cells degrade and release intracellular contents under specific conditions by endogenous enzymes such as proteases, nucleases and lipid enzymes. Protein-rich baker's yeast is widely used to produce yeast extract in food industry, however, the molecular mechanism related to baker's yeast autolysis is still unclear. In this study, RNA-seq technology and
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Molecular mechanism of the response of Zygosaccharomyces rouxii to D-fructose stress by the glutathione metabolism pathway. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-18 Hong Liu,Xin Li,Jingzhi Deng,Lingyan Dai,Wei Liu,Bailing Pan,Chengtao Wang,Dongjie Zhang,Zhijiang Li
Zygosaccharomyces rouxii produces high levels of 4-hydroxy-2,5-dimethyl-3(2H)-furanone in YPD medium supplemented with 120 g/L D-fructose and 180 g/L NaCl after 5 d. D-fructose has a stress effect on Z. rouxii, and GSH-Px is a main enzyme involved in the defense of Z. rouxii against oxygen stress according to our previous report. In order to further explore the molecular mechanism of the glutathione
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Promoters for lipogenesis-specific downregulation in Yarrowia lipolytica. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-13 Annapurna Kamineni,Shuyan Chen,Gamuchirai Chifamba,Vasiliki Tsakraklides
AbstractYarrowia lipolytica is a non-conventional yeast with potential applications in the biofuel and biochemical industries. It is an oleaginous yeast that accumulates lipids when it encounters nutrient limitation in the presence of excess carbon. Its molecular toolbox includes promoters for robust constitutive expression, regulated expression through the addition of media components and inducible
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Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-07-02 Zhiqiang Wen,Sufang Zhang,Chuks Kenneth Odoh,Mingjie Jin,Zongbao K Zhao
The red yeast Rhodosporidium toruloides naturally produces microbial lipids and carotenoids. In the past decade or so, many studies demonstrated R. toruloides as a promising platform for lipid production owing to its diverse substrate appetites, robust stress resistance and other favorable features. Also, significant progresses have been made in genome sequencing, multi-omic analysis and genome-scale
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¡Viva la mitochondria!: harnessing yeast mitochondria for chemical production. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-27 Lisset Duran,José Montaño López,José L Avalos
The mitochondria, often referred to as the powerhouse of the cell, offer a unique physicochemical environment enriched with a distinct set of enzymes, metabolites and cofactors ready to be exploited for metabolic engineering. In this review, we discuss how the mitochondrion has been engineered in the traditional sense of metabolic engineering or completely bypassed for chemical production. We then
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Ethanol and H2O2 stresses enhance lipid production in an oleaginous Rhodotorula toruloides thermotolerant mutant L1-1. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-18 Chih-Chan Wu,Takao Ohashi,Ryo Misaki,Savitree Limtong,Kazuhito Fujiyama
Stress tolerance is a desired characteristic of yeast strains for industrial applications. Stress tolerance has been well described in Saccharomyces yeasts but has not yet been characterized in oleaginous Rhodotorula yeasts even though they are considered promising platforms for lipid production owing to their outstanding lipogenicity. In a previous study, the thermotolerant strain L1–1 was isolated
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A homologous overexpression system to study roles of drug transporters in Candida glabrata. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-03 Sonam Kumari,Mohit Kumar,Nitesh Kumar Khandelwal,Ajay Kumar Pandey,Priyanka Bhakt,Rupinder Kaur,Rajendra Prasad,Naseem A Gaur
Considering the relevance of drug transporters belonging to ABC and MFS superfamilies in pathogenic Candida species, there has always been a need to have an overexpression system where these membrane proteins for functional analysis could be expressed in a homologous background. We could address this unmet need by constructing a highly drug-susceptible Candida glabrata strain deleted in seven dominant
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Neither 1G nor 2G fuel ethanol: setting the ground for a sugarcane-based biorefinery using an iSUCCELL yeast platform. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-13 Pamela Magalí Bermejo,Vijayendran Raghavendran,Andreas Karoly Gombert
First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challenges inherent to these processes could be resolved by efficiently separating and partially
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The virulence factor urease and its unexplored role in the metabolism of Cryptococcus neoformans. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-06-03 Barbra Toplis,Caylin Bosch,Ilan S Schwartz,Chris Kenyon,Teun Boekhout,John R Perfect,Alfred Botha
Cryptococcal urease is believed to be important for the degradation of exogenous urea that the yeast encounters both in its natural environment and within the human host. Endogenous urea produced by the yeast's own metabolic reactions, however, may also serve as a substrate for the urease enzyme. Using wild-type, urease-deletion mutant and urease-reconstituted strains of Cryptococcus neoformans H99
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The impact of CUP1 gene copy-number and XVI-VIII/XV-XVI translocations on copper and sulfite tolerance in vineyard Saccharomyces cerevisiae strain populations. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-21 Giulia Crosato,Chiara Nadai,Milena Carlot,Juliano Garavaglia,Denise Righetto Ziegler,Rochele Cassanta Rossi,Juliana De Castilhos,Stefano Campanaro,Laura Treu,Alessio Giacomini,Viviana Corich
In wine production, sulfites are widely used as antimicrobials and antioxidants, whereas copper is associated with fungicides and wine fining treatments. Therefore, wine yeasts are constantly exposed to these agents. Copper tolerance is related to the copy number of the CUP1 gene, encoding for a metallothionein involved in copper detoxification. In wine yeasts, sulfite resistance mainly depends on
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Transcription factor Liv4 is required for growth and pathogenesis of Cryptococcus neoformans. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Jiu Yi,Junjun Sang,Jingyu Zhao,Lei Gao,Yali Yang,Lei Yan,Chao Zhang,Weihua Pan,Guizhen Wang,Wanqing Liao
Cryptococcus neoformans is an important invasive fungal pathogen that causes life-threatening meningoencephalitis in humans. Its biological and pathogenic regulatory mechanisms remain largely unknown, particularly due to the presence of those core transcription factors (TFs). Here, we conducted a detailed characterization of the TF Liv4 in the biology and virulence of C. neoformans. Deletion of TF
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Pinostrobin suppresses the Ca2+-signal-dependent growth arrest in yeast by inhibiting the Swe1-mediated G2 cell-cycle regulation. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-13 Jumpol Sopanaporn,Sirinporn Suksawatamnuay,Amanulia Sardikin,Rittirat Lengwittaya,Warinthorn Chavasiri,Tokichi Miyakawa,Chulee Yompakdee
Pinostrobin, a flavonoid compound known for its diverse pharmacological actions, including anti-leukemic and anti-inflammatory activities, has been repeatedly isolated by various screenings, but its action mechanism is still obscure. Previously, pinostrobin was rediscovered in our laboratory using a yeast-based assay procedure devised specifically for the inhibitory effect on the activated Ca2+ signaling
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20 years a-publishing - the development of FEMS Yeast Research. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 John Morrissey
‘Publish or Perish’ is a well-known aphorism within the academic community; every Ph.D. student undergoes a moment of realisation that their academic career is dependent on their publication output. The pressure continues with post-doctoral researchers seeking the paper that will propel them towards their next position and faculty members knowing that tenure or promotion is dependent on a strong research
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Quantitative assessment of chaperone binding to amyloid aggregates identifies specificity of Hsp40 interaction with yeast prion fibrils. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-07 Yury A Barbitoff,Andrew G Matveenko,Stanislav A Bondarev,Evgeniia M Maksiutenko,Alexandra V Kulikova,Galina A Zhouravleva
Yeast self-perpetuating protein aggregates (yeast prions) provide a framework to investigate the interaction of misfolded proteins with the protein quality control machinery. The major component of this system that facilitates propagation of all known yeast amyloid prions is the Hsp104 chaperone that catalyzes fibril fragmentation. Overproduction of Hsp104 cures some yeast prions via a fragmentation-independent
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Komagataella phaffii YPS1-5 encodes the alpha-factor degrading protease Bar1. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Lina Heistinger,Brigitte Gasser,Diethard Mattanovich
Yeast mating pheromones are small secreted peptides required for efficient mating between cells of opposite mating type. Pheromone gradients allow the cells to detect potential mating partners. Secreted pheromone degrading proteases steepen local gradients and allow fast recovery from the pheromone signal. The methylotrophic yeast Komagataella phaffii is a preferentially haploid species. Only under
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The antifungal pipeline: the need is established. Are there new compounds? FEMS Yeast Res. (IF 3.193) Pub Date : 2020-04-30 Dongmei Li,Xiaodong She,Richard Calderone
Our review summarizes and compares the temporal development (eras) of antifungal drug discovery as well as antibacterial ventures. The innovation gap that occurred in antibacterial discovery from 1960 to 2000 was likely due to tailoring of existing compounds to have better activity than predecessors. Antifungal discovery also faced innovation gaps. The semi-synthetic antibiotic era was followed closely
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Involvement of Pca1 in ROS-mediated apoptotic cell death induced by alpha-thujone in the fission yeast (Schizosaccharomyces pombe). FEMS Yeast Res. (IF 3.193) Pub Date : 2020-04-29 Hizlan Hincal Agus,Gizem Kok,Ezgi Derinoz,Didem Oncel,Sedanur Yilmaz
Alpha-thujone, widely used in beverages (1–5 mg/kg), is known to have cytotoxic effects, but the mode of action and the role of potential apoptotic proteins in yeast cell death should be unraveled. In this study, we used Schizosaccharomyces pombe, which is a promising unicellular model organism in mechanistic toxicology and cell biology, to investigate the involvement of pro-apoptotic factors in a
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Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Sofie Lodens,Sophie L K W Roelants,Goedele Luyten,Robin Geys,Pieter Coussement,Sofie L De Maeseneire,Wim Soetaert
Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was
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Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Eva Vaňková,Petra Kašparová,Nikola Dulíčková,Václav Čeřovský
Candida albicans has several virulence factors at its disposal, including yeast-hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to its pathogenesis. We used synthetic derivative LL-III/43 of antimicrobial peptide lasioglossin LL-III to enhance effect of azoles on attenuation of C. albicans virulence factors. LL-III/43 was
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The Lipomyces starkeyi gene Ls120451 encodes a cellobiose transporter that enables cellobiose fermentation in Saccharomyces cerevisiae. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Jorg C de Ruijter,Kiyohiko Igarashi,Merja Penttilä
Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through the heterologous expression of sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened a selection
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An update on the diversity, ecology and biogeography of the Saccharomyces genus FEMS Yeast Res. (IF 3.193) Pub Date : 2020-03-20 Alsammar H, Delneri D.
ABSTRACTSaccharomyces cerevisiae is the most extensively studied yeast and, over the last century, provided insights on the physiology, genetics, cellular biology and molecular mechanisms of eukaryotes. More recently, the increase in the discovery of wild strains, species and hybrids of the genus Saccharomyces has shifted the attention towards studies on genome evolution, ecology and biogeography,
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Natural extract and its fractions isolated from the marine bacterium Pseudoalteromonas flavipulchra STILL-33 have antioxidant and antiaging activities in Schizosaccharomyces pombe FEMS Yeast Res. (IF 3.193) Pub Date : 2020-03-16 Prastya M, Astuti R, Batubara I, et al.
ABSTRACTInvestigations into the potential for pharmacological inhibition of the aging process and the onset of age-related disease are increasingly garnering attention. Here, we analyzed the antiaging properties of natural compounds derived from several marine bacteria in vitro and in vivo using the fission yeast Schizosaccharomyces pombe. The Pseudoalteromonas flavipulchra STILL-33 extract exhibited
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The Epithelial adhesin 1 tandem repeat region mediates protein display through multiple mechanisms. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Colin J Raposo,Kyle A McElroy,Stephen M Fuchs
The pathogenic yeast Candida glabrata is reliant on a suite of cell surface adhesins that play a variety of roles necessary for transmission, establishment and proliferation during infection. One particular adhesin, Epithelial Adhesin 1 [Epa1p], is responsible for binding to host tissue, a process which is essential for fungal propagation. Epa1p structure consists of three domains: an N-terminal intercellular
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Discovering and genotyping genomic structural variations by yeast genome synthesis and inducible evolution FEMS Yeast Res. (IF 3.193) Pub Date : 2020-03-19 Chen S, Xie Z, Yuan Y.
ABSTRACTGenomic structural variations (SVs) promote the evolution of Saccharomyces cerevisiae, and play an important role in phenotypic diversities. Yeast genomic structures can be remodeled by design and bottom-up synthesis. The synthesis of yeast genome creates novel copy number variations (CNVs) and SVs and develops new strategies to discover gene functions. Further, an inducible evolution system
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redLips: a comprehensive mechanistic model of the lipid metabolic network of yeast. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-02-18 S Tsouka,V Hatzimanikatis
Over the last decades, yeast has become a key model organism for the study of lipid biochemistry. Because the regulation of lipids has been closely linked to various physiopathologies, the study of these biomolecules could lead to new diagnostics and treatments. Before the field can reach this point, however, sufficient tools for integrating and analyzing the ever-growing availability of lipidomics
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Yeast Mycocins: a great potential for application in health. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-05-01 Bruna L Nascimento,Mateus F Delabeneta,Lana Rubia B Rosseto,Daniele S B Junges,Ana Paula Paris,Cristiane Persel,Rinaldo F Gandra
Mycocins have demonstrated inhibition of fungi, bacteria, parasites and viruses, in addition to being studied as epidemiological markers and in the development of vaccines. They are defined as extracellular proteins or glycoproteins with different activities, the main mechanism of action being the inhibition of β-glucan synthesis in the cell wall of sensitive strains. Given the resistance problems
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Extracting novel hypotheses and findings from RNA-seq data. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-02-03 Tyler Doughty,Eduard Kerkhoven
Over the past decade, improvements in technology and methods have enabled rapid and relatively inexpensive generation of high-quality RNA-seq datasets. These datasets have been used to characterize gene expression for several yeast species and have provided systems-level insights for basic biology, biotechnology and medicine. Herein, we discuss new techniques that have emerged and existing techniques
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Yeast synthetic biology for designed cell factories producing secretory recombinant proteins. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-02-03 Eun Jung Thak,Su Jin Yoo,Hye Yun Moon,Hyun Ah Kang
Yeasts are prominent hosts for the production of recombinant proteins from industrial enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways between these unicellular eukaryotic microorganisms and higher eukaryotic organisms has made them a preferential host to produce secretory recombinant proteins. However, there are several bottlenecks, in terms of quality and
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Into the wild: new yeast genomes from natural environments and new tools for their analysis. FEMS Yeast Res. (IF 3.193) Pub Date : 2020-02-03 D Libkind,D Peris,F A Cubillos,J L Steenwyk,D A Opulente,Q K Langdon,A Rokas,C T Hittinger
Genomic studies of yeasts from the wild have increased considerably in the last few years. This revolution has been fueled by advances in high-throughput sequencing technologies and a better understanding of yeast ecology and phylogeography, especially for biotechnologically important species. The present review aims to first introduce new bioinformatic tools available for the generation and analysis