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Bronchoalveolar lavage fluid IMMY Sona Aspergillus lateral-flow assay for the diagnosis of invasive pulmonary aspergillosis: a prospective, real life evaluation Med. Mycol. (IF 2.822) Pub Date : 2021-01-21 P Arkell; S Mahboobani; R Wilson; N Fatania; M Coleman; A M Borman; E M Johnson; D P H Armstrong-James; A Abdolrasouli
Prompt and reliable diagnosis of invasive pulmonary aspergillosis (IPA) is essential for early initiation of antifungal therapy. We evaluated bronchoalveolar lavage (BAL) fluid IMMY Sona Aspergillus lateral-flow assay (IMMY LFA) in 92 individuals with suspected pulmonary infection. Sensitivity and specificity (vs. host factor but no IPA) of BAL IMMY LFA for diagnosis of IPA in individuals with any
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False-positive galactomannan antigen testing in pulmonary nocardiosis. Med. Mycol. (IF 2.822) Pub Date : 2020-09-17 Arnon Haran,Violeta Temper,Marc Assous,Michael Bergel,Noga Chahanian,Hila Elinav,Maya Korem
Early diagnosis of invasive aspergillosis (IA) is facilitated by detection of galactomannan (GM) in serum and bronchoalveolar lavage fluid (BALF) using an enzyme-linked immunosorbent assay (ELISA). Although accurate, false positive results have been reported with these tests in numerous contexts. We report for the first time the occurrence of false positive GM ELISA due to nocardiosis, initially in
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Sporothrix brasiliensis on cats with skin ulcers in Southern Brazil. Med. Mycol. (IF 2.822) Pub Date : 2020-09-17 Andréia Spanamberg,Ricardo Araujo,Ana Paulo Ravazzolo,David Driemeier,Rosane Maria Sordi Driemeier,Laerte Ferreiro
Sporotrichosis is a disease caused by thermally dimorphic fungi belonging to the Sporothrix schenckii complex. We report the occurrence of multiple cases of sporotrichosis in cats in Porto Alegre (RS), Brazil, from 2015 to 2019. A set of 21 fungal isolates were collected and the partial sequence of calmodulin (CAL) gene compared. All isolates were identified at phenotypic and molecular level as S.
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The assimilation of different carbon sources in Candida albicans: Fitness and pathogenicity. Med. Mycol. (IF 2.822) Pub Date : 2020-09-17 Bronwyn Lok,Mowaffaq Adam Ahmad Adam,Laina Zarisa Mohd Kamal,Nwakpa Anthony Chukwudi,Rosline Sandai,Doblin Sandai
Candida albicans is a commensal yeast commonly found on the skin and in the body. However, in immunocompromised individuals, the fungi could cause local and systemic infections. The carbon source available plays an important role in the establishment of C. albicans infections. The fungi's ability to assimilate a variety of carbon sources plays a vital role in its colonization, and by extension, its
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Coccidioidomycosis in llamas and alpacas diagnosed at the University of California, Davis (1990-2016). Med. Mycol. (IF 2.822) Pub Date : 2020-09-17 S E Grayzel,G R Thompson,B Martínez-López,J E Dechant,I McHardy,J E Sykes
Given the predisposition of South American camelids to coccidioidomycosis, we sought to describe the disease presentation in alpacas and llamas and identify potential risk factors for these species. The records of 224 llamas and alpacas that were tested for Coccidioides infection using immunodiffusion serology at the Coccidioidomycosis Serology Laboratory of the University of California, Davis, between
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On the lineage of Aspergillus fumigatus isolates in common laboratory use. Med. Mycol. (IF 2.822) Pub Date : 2020-09-17 Margherita Bertuzzi,Norman van Rhijn,Sven Krappmann,Paul Bowyer,Michael J Bromley,Elaine M Bignell
AbstractThe origin of isolates routinely used by the community of Aspergillus fumigatus researchers is periodically a matter of intense discussion at our centre, as the construction of recombinant isolates have sometimes followed convoluted routes, the documentation describing their lineages is fragmented, and the nomenclature is confusing. As an aide memoir, not least for our own benefit, we submit
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Antifungal effect of anthraquinones against Cryptococcus neoformans: detection of synergism with amphotericin B. Med. Mycol. (IF 2.822) Pub Date : 2020-09-14 Raimunda Sâmia Nogueira Brilhante,Géssica Dos Santos Araújo,Xhaulla Maria Quariguasi Cunha Fonseca,Glaucia Morgana de Melo Guedes,Lara de Aguiar,Débora de Souza Collares Maia Castelo-Branco,Rossana de Aguiar Cordeiro,José Júlio Costa Sidrim,Waldemiro Aquino Pereira Neto,Marcos Fábio Gadelha Rocha
The emergence of tolerant Cryptococcus neoformans strains to antifungals has been described. It has directed researchers to screen for new antimicrobial compounds. In this context, several plant-derived compounds, such as anthraquinones (aloe emodin, barbaloin, and chrysophanol), have been investigated for their antimicrobial properties. This study aimed to evaluate the in vitro effect of aloe emodin
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Prospective observational study of respiratory Aspergillus colonization or disease in patients with various stages of chronic obstructive pulmonary disease utilizing culture versus nonculture techniques. Med. Mycol. (IF 2.822) Pub Date : 2020-09-14 Sarmad Waqas,Katie Dunne,Alida Fe Talento,Graham Wilson,Ignacio Martin-Loeches,Joseph Keane,Thomas R Rogers
Chronic obstructive pulmonary disease (COPD) patients have been recognized to be at increased risk of Aspergillus spp. colonization, which may progress to invasive pulmonary aspergillosis (IPA). The objective of this study was to determine the frequency of Aspergillus colonization, or disease, in a cohort of COPD patients. A prospective observational study was undertaken to determine Aspergillus colonization
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Pulmonary aspergillosis in critically ill patients with Coronavirus Disease 2019 (COVID-19). Med. Mycol. (IF 2.822) Pub Date : 2020-09-10 Damien Dupont,Jean Menotti,Jean Turc,Charline Miossec,Florent Wallet,Jean-Christophe Richard,Laurent Argaud,Sylvie Paulus,Martine Wallon,Florence Ader,Florence Persat
AbstractOccurrence of putative invasive pulmonary aspergillosis was screened in 153 consecutive adult intensive care unit (ICU) patients with respiratory samples addressed for mycological diagnosis during a 6-week period at the emergence of coronavirus disease 2019 (COVID-19) pandemic. Positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) was observed
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Future challenges and chances in the diagnosis and management of invasive mould infections in cancer patients. Med. Mycol. (IF 2.822) Pub Date : 2020-09-08 Jörg Janne Vehreschild,Philipp Koehler,Frédéric Lamoth,Juergen Prattes,Christina Rieger,Bart J A Rijnders,Daniel Teschner
AbstractDiagnosis, treatment, and management of invasive mould infections (IMI) are challenged by several risk factors, including local epidemiological characteristics, the emergence of fungal resistance and the innate resistance of emerging pathogens, the use of new immunosuppressants, as well as off-target effects of new oncological drugs. The presence of specific host genetic variants and the patient's
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Comparison of the characteristics of patients with invasive infections and noninvasive infections caused by Trichosporon asahii. Med. Mycol. (IF 2.822) Pub Date : 2020-09-02 Jinyeong Kim,Min Jae Kim,Yong Pil Chong,Sung-Han Kim,Sang-Ho Choi,Sang-Oh Lee,Jun Hee Woo,Yang Soo Kim,Jiwon Jung
We performed retrospective study to identify the characteristics of invasive Trichosporon asahii infection. A total of 102 patients with T. asahii were identified including 18 (18%) with invasive infection. Invasive infection was associated with indwelling central venous catheter (94% vs 54%, P = .001), prior antifungal agent use (50% vs 18%, P = .01), hematologic malignancy (33% vs 7%, P = .006),
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Antifungal resistance-modifying multiplexing action of Momordica charantia protein and phosphorylated derivatives on the basis of growth-dependent gene coregulation in Candida albicans. Med. Mycol. (IF 2.822) Pub Date : 2020-09-01 Qiao Yuan-Biao,Zhang Lan-Fang,Qiao Qi,Niu Jia-Hui,Ren Ze-Mei,Yang Hai-Mei,Zhu Chen-Chen,Pan Hong-Ju,Duan Nan-Nan,Li Qing-Shan
Fungal growth-dependent gene coregulation is strongly implicated in alteration of gene-encoding target proteases ruling with an antifungal resistance niche and biology of resistant mutants. On the basis of multi-alterative processes in this platform, the resistance-modifying strategy is designed in ketoconazole resistant Candida albicans and evaluated with less selective Momordica charantia protein
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Diphenyl diselenide and its interaction with antifungals against Aspergillus spp. Med. Mycol. (IF 2.822) Pub Date : 2020-08-25 Aryse Martins Melo,Vanice Rodrigues Poester,Mariana Trapaga,Cristina Wayne Nogueira,Gilson Zeni,Marife Martinez,Gabriele Sass,David A Stevens,Melissa Orzechowski Xavier
Given the few antifungal classes available to treat aspergillosis, this study aimed to evaluate the in vitro antifungal activity of diphenyl diselenide (PhSe)2 alone and in combination with classical antifungals against Aspergillus spp., and its in vivo activity in a systemic experimental aspergillosis model. We performed in vitro broth microdilution assay of (PhSe)2 against 32 Aspergillus isolates;
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Cellular and molecular insights on the regulation of innate immune responses to experimental aspergillosis in chicken and turkey poults. Med. Mycol. (IF 2.822) Pub Date : 2020-08-25 Tobias Vahsen,Laura Zapata,Rodrigo Guabiraba,Elise Melloul,Nathalie Cordonnier,Françoise Botterel,Jacques Guillot,Pascal Arné,Veronica Risco-Castillo
Across the world, many commercial poultry flocks and captive birds are threatened by infection with Aspergillus fumigatus. Susceptibility to aspergillosis varies among birds; among galliform birds specifically, morbidity and mortality rates seem to be greater in turkeys than in chickens. Little is known regarding the features of avian immune responses after inhalation of Aspergillus conidia, and to
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Antifungal activity of deferiprone and EDTA against Sporothrix spp.: Effect on planktonic growth and biofilm formation. Med. Mycol. (IF 2.822) Pub Date : 2020-08-24 Raimunda Sâmia Nogueira Brilhante,Anderson da Cunha Costa,Vandbergue Santos Pereira,Mirele Rodrigues Fernandes,Jonathas Sales de Oliveira,Anderson Messias Rodrigues,Zoilo Pires Camargo,Waldemiro de Aquino Pereira-Neto,José Júlio Costa Sidrim,Marcos Fábio Gadelha Rocha
The present study evaluated the antifungal activity of the chelators deferiprone (DFP) and ethylenediaminetetraacetic acid (EDTA) and their effect on biofilm formation of the S. schenckii complex. Eighteen strains of Sporothrix spp. (seven S. brasiliensis, three S. globosa, three S. mexicana and five Sporothrix schenckii sensu stricto) were used. Minimum inhibitory concentration (MIC) values for EDTA
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The skin mycobiome of an astronaut during a 1-year stay on the International Space Station. Med. Mycol. (IF 2.822) Pub Date : 2020-08-24 Takashi Sugita,Takashi Yamazaki,Otomi Cho,Satoshi Furukawa,Chiaki Mukai
AbstractAnalysis of the skin mycobiome of an astronaut during a 1-year stay on the International Space Station (ISS) revealed an increased relative abundance of Malassezia restricta and level of Malassezia colonization, and the presence of Cyberlindnera jadinii and Candida boidinii, uncommon skin mycobiome taxa. Similar observations were made in astronauts during a 6-month stay on the ISS (Med Mycol
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Under nonlimiting iron conditions pyocyanin is a major antifungal molecule, and differences between prototypic Pseudomonas aeruginosa strains. Med. Mycol. (IF 2.822) Pub Date : 2020-08-22 Gabriele Sass,Hasan Nazik,Paulami Chatterjee,David A Stevens
Airways of immunocompromised patients, or individuals with cystic fibrosis (CF), are common ground for Pseudomonas aeruginosa and Aspergillus fumigatus infections. Hence, in such a microenvironment both pathogens compete for resources. While under limiting iron conditions the siderophore pyoverdine is the most effective antifungal P. aeruginosa product, we now provide evidence that under nonlimiting
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Natural alkaloid tryptanthrin exhibits novel anticryptococcal activity. Med. Mycol. (IF 2.822) Pub Date : 2020-08-21 Chi-Jan Lin,Ya-Lin Chang,Yu-Liang Yang,Ying-Lien Chen
Cryptococcal meningitis is a prevalent invasive fungal infection that causes around 180 000 deaths annually. Currently, treatment for cryptococcal meningitis is limited and new therapeutic options are needed. Historically, medicinal plants are used to treat infectious and inflammatory skin infections. Tryptanthrin is a natural product commonly found in these plants. In this study, we demonstrated that
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Antifungal efficacy of atorvastatin-containing emulgel in the treatment of oral and vulvovaginal candidiasis. Med. Mycol. (IF 2.822) Pub Date : 2020-08-21 Ari Soares de Oliveira Neto,Israel Lucas Antunes Souza,Maria Eliza Samuel Amorim,Thalita de Freitas Souza,Vinicius Novaes Rocha,Rene Oliveira do Couto,Rodrigo Luiz Fabri,Marcelo Gonzaga de Freitas Araújo
Drug repositioning has been an important ally in the search for new antifungal drugs. Statins are drugs that act to prevent sterol synthesis in both humans and fungi and for this reason they are promissory candidates to be repositioned to treat mycoses. In this study we evaluated the antifungal activity of atorvastatin by in vitro tests to determine the minimum inhibitory concentration against azole
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Effect of zinc pyrithione shampoo treatment on skin commensal Malassezia. Med. Mycol. (IF 2.822) Pub Date : 2020-08-12 Cheryl Leong,Joyce Wang,Min Jet Toi,Yuen In Lam,Joleen P Z Goh,Shi Mun Lee,Thomas L Dawson
Malassezia restricta and Malassezia globosa are lipid dependent commensal yeasts associated with dandruff. Antifungal actives such as zinc pyrithione are commonly used in antidandruff shampoos, although their efficacy is not clearly demonstrated. In this study, we assessed the efficacy of antifungal treatments on scalp Malassezia via a combination of culturomic and genomic detection methods. Zinc pyrithione
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Evaluating Candida albicans biofilm formation in silkworms. Med. Mycol. (IF 2.822) Pub Date : 2020-08-11 Yasuhiko Matsumoto,Sanae Kurakado,Takashi Sugita
Candida albicans is a pathogenic fungus that causes deep mycosis in immunocompromised patients and forms a biofilm on catheter surfaces. Here we showed that C. albicans infection of silkworms led to biofilm formation on the surface of polyurethane fibers, a catheter substrate material, while inside the silkworm body. Silkworms inserted with polyurethane fibers survived for at least 48 hours. When silkworms
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Peptidorhamanomannan: A surface fungal glycoconjugate from Scedosporium aurantiacum and Scedosporium minutisporum and its recognition by macrophages. Med. Mycol. (IF 2.822) Pub Date : 2020-08-07 Jardel Vieira de Meirelles,Mariana Ingrid Dutra da Silva Xisto,Rodrigo Rollin-Pinheiro,Rodrigo Vassoler Serrato,Rosa Maria Tavares Haido,Eliana Barreto-Bergter
The genus Scedosporium is composed of clinically relevant fungal species, such as Scedosporium aurantiacum, Scedosporium apiospermum, and Scedosporium boydii. Surface molecules have been described that play crucial roles in fungi-macrophage interaction, and many of them are pathogen-associated molecular patterns (PAMPs). The present study aims to characterize peptidoglycans obtained from Scedosporium
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A new pleosporalean fungus isolated from superficial to deep human clinical specimens. Med. Mycol. (IF 2.822) Pub Date : 2020-07-27 Nicomedes Valenzuela-Lopez,M Teresa Martin-Gomez,Ibai Los-Arcos,Alberto M Stchigel,Josep Guarro,José F Cano-Lira
Human infections by pleosporalean fungi (class Dothideomycetes, phylum Ascomycota) are rarely reported. Because their identification is challenging using morphological characterization, several phylogenetic markers must be sequenced for an accurate identification and taxonomical placement of the isolates. Three isolates of clinical origin were phenotypically characterized, but due to the absence of
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Candida tropicalis RON1 is required for hyphal formation, biofilm development, and virulence but is dispensable for N-acetylglucosamine catabolism. Med. Mycol. (IF 2.822) Pub Date : 2020-07-25 Yu-De Song,Chih-Chieh Hsu,Shi Qian Lew,Ching-Hsuan Lin
NDT80-like family genes are highly conserved across a large group of fungi, but the functions of each Ndt80 protein are diverse and have evolved differently among yeasts and pathogens. The unique NDT80 gene in budding yeast is required for sexual reproduction, whereas three NDT80-like genes, namely, NDT80, REP1, and RON1, found in Candida albicans exhibit distinct functions. Notably, it was suggested
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Transcriptomic profiling identifies novel transcripts, isomorphs, and noncoding RNAs in Paracoccidioides brasiliensis. Med. Mycol. (IF 2.822) Pub Date : 2020-07-21 Fabiano B Menegidio,David Aciole Barbosa,Valquíria C Alencar,Renata O Vilas Boas,Regina Costa de Oliveira,Daniela L Jabes,Luiz R Nunes
This paper describes a transcriptomic profiling of Paracoccidioides brasiliensis (Pb) performed with the aid of an RNA-seq-based approach, aimed at characterizing the general transcriptome in this human pathogenic fungus, responsible for paracoccidioidomycosis (PCM). Results confirm that ∼75% of the genes currently annotated in the P. brasiliensis genome are, in fact, transcribed in vivo and that ∼19%
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Epidemiology of candidemia in Shiraz, southern Iran: A prospective multicenter study (2016-2018). Med. Mycol. (IF 2.822) Pub Date : 2020-07-21 Amir Arastehfar,Samira Yazdanpanah,Mina Bakhtiari,Wenjie Fang,Weihua Pan,Shahram Mahmoudi,Keyvan Pakshir,Farnaz Daneshnia,Teun Boekhout,Macit Ilkit,David S Perlin,Kamiar Zomorodian,Farid Zand
Systematic candidemia studies, especially in southern Iran, are scarce. In the current prospective study, we investigated candidemia in three major healthcare centers of Shiraz, the largest city in southern Iran. Yeast isolates from blood and other sterile body fluids were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and subjected to antifungal susceptibility
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8-Hydroxyquinoline 1,2,3-triazole derivatives with promising and selective antifungal activity. Med. Mycol. (IF 2.822) Pub Date : 2020-07-21 Nailí Moreira da Silva,Caroline de Bem Gentz,Paula Reginatto,Thaís Helena Maciel Fernandes,Taís Fernanda Andrzejewski Kaminski,William Lopes,Priscilla M Quatrin,Marilene Henning Vainstein,Maxwel Adriano Abegg,Marcela Silva Lopes,Alexandre Meneghello Fuentefria,Saulo Fernandes de Andrade
Fungal infections that affect humans and plants have increased significantly in recent decades. However, these pathogens are still neglected when compared to other infectious agents. Due to the high prevalence of these infections, the need for new molecules with antifungal potential is recognized, as pathogenic species are developing resistance to the main drugs available. This work reports the design
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Pharmacological serum concentrations of epinephrine and norepinephrine do not affect growth rate, morphogenesis, stress tolerance, and virulence of Candida albicans. Med. Mycol. (IF 2.822) Pub Date : 2020-07-17 Sebastian Wurster,Sebastian Lyos,Nathaniel D Albert,Dimitrios P Kontoyiannis
AbstractVasopressors are frequently given in hemodynamically unstable patients with severe Candida sepsis. While catecholamines can aggravate sepsis-induced immune dysfunction and modulate bacterial virulence traits, their impact on fungal pathogenicity is poorly understood. Using IncuCyte time-lapse microscopy and a fruit fly candidiasis model, we studied growth rates, morphogenesis, stress tolerance
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Oxidative stress induced by piperine leads to apoptosis in Candida albicans. Med. Mycol. (IF 2.822) Pub Date : 2020-07-13 Archana Thakre,Vyankatesh Jadhav,Rubina Kazi,Amruta Shelar,Rajendra Patil,Kiran Kharat,Gajanan Zore,S Mohan Karuppayil
Candida albicans is a member of pathogens with potential drug resistance threat that needs novel chemotherapeutic strategies. Considering the multifarious biological activities including bioenhancer activity, anti-Candida potential of piperine was evaluated against planktonic/biofilm and hyphal growth of C. albicans alone or in combination as a synergistic agent with fluconazole. Piperine inhibits
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High detection rate of azole-resistant Aspergillus fumigatus after treatment with azole antifungal drugs among patients with chronic pulmonary aspergillosis in a single hospital setting with low azole resistance. Med. Mycol. (IF 2.822) Pub Date : 2020-07-09 Keita Takeda,Junko Suzuki,Akira Watanabe,Teppei Arai,Tomohiro Koiwa,Kyota Shinfuku,Osamu Narumoto,Masahiro Kawashima,Takeshi Fukami,Atsuhisa Tamura,Hideaki Nagai,Hirotoshi Matsui,Katsuhiko Kamei
The prevalence of azole-resistant Aspergillus fumigatus (ARAF) among chronic pulmonary aspergillosis (CPA) patients treated with azoles in Japan is unknown. The aim of this study was to determine the detection rate of ARAF in isolates from CPA patients who were treated with azoles for varying durations. The potential mechanism of acquiring resistance was examined by sequencing cyp51A and hmg1, two
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Three-gene phylogeny of the genus Arthroderma: Basis for future taxonomic studies. Med. Mycol. (IF 2.822) Pub Date : 2020-07-08 Steven Hainsworth,Ivana Kučerová,Rahul Sharma,Connie F Cañete-Gibas,Vit Hubka
Arthroderma is the most diverse genus among dermatophytes encompassing species occurring in soil, caves, animal burrows, clinical material and other environments. In this study, we collected ex-type, reference and authentic strains of all currently accepted Arthroderma species and generated sequences of three highly variable loci (ITS rDNA, β-tubulin, and translation elongation factor 1-α). The number
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Potential use of Nikkomycin Z as an anti- Sporothrix spp. drug. Med. Mycol. (IF 2.822) Pub Date : 2020-07-07 Vanice Rodrigues Poester,Lívia Silveira Munhoz,David Larwood,Marife Martinez,David A Stevens,Melissa Orzechowski Xavier
Sporotrichosis, the most common subcutaneous mycosis in several countries, is caused by the dimorphic fungus, Sporothrix spp. Given some limitations in the treatment of this disease, and the high potential of nikkomycin Z (NikZ) as an antifungal against dimorphic fungi, this study aimed to evaluate the in vitro susceptibility of Sporothrix spp. to NikZ alone and with the drug of choice, itraconazole
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Epidemiology of bloodstream Candida species in a Spanish tertiary care hospital as a guide for implementation of T2MR (T2CANDIDA®) for rapid diagnosis of candidemia. Med. Mycol. (IF 2.822) Pub Date : 2020-07-07 Iker Falces-Romero,María Pilar Romero-Gómez,Francisco Moreno-Ramos,Jesús Mingorance,Julio García-Rodríguez,Emilio Cendejas-Bueno
The aim of this work was to study the epidemiology of candidemia in our hospital in order to determine whether the T2MR system might be a useful tool for early diagnosis of candidemia in selected units. We perform a retrospective review of all candidemia episodes registered in the last 12 years in selected units of our hospital in adult and pediatric patients. Candida species and antifungal susceptibility
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Clinical mycology today: A synopsis of the mycoses study group education and research consortium (MSGERC) second biennial meeting, September 27-30, 2018, Big Sky, Montana, a proposed global research agenda. Med. Mycol. (IF 2.822) Pub Date : 2020-05-14 Peter G Pappas,David R Boulware,Dimitrios P Kontoyiannis,Marisa H Miceli,Luis Ostrosky-Zeichner,Andrej Spec,George R Thompson,Sharon Chen,John R Perfect,
The second biennial meeting of the Mycoses Study Group Education and Research Consortium (MSGERC) took place in Big Sky, Montana, from September 25 through 28, 2018. To the clinician and clinician/educator, this meeting serves as an up-to-date review of critical issues in mycology provided by the world's leading experts. To the clinical and translational researcher, it provides an opportunity to share
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Paeonol alleviates dextran sodium sulfate induced colitis involving Candida albicans-associated dysbiosis. Med. Mycol. (IF 2.822) Pub Date : 2020-06-29 Yuzhu Ge,Min Pan,Chuanfeng Zhang,Changzhong Wang,Kelong Ma,Guiming Yan,Tianming Wang,Daqiang Wu,Jing Shao
Inflammatory bowel disease (IBD), which consists of ulcerative colitis (UC) and Crohn's disease (CD), is a chronic inflammatory disorder of the gastrointestinal tract. Occurrence and development of UC have been associated with multiple potential causative factors, which include fungal dysbiosis. Growing evidence reveals that Candida albicans-associated dysbiosis is correlated with clinical deterioration
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Central nervous system candidiasis beyond neonates: Lessons from a nationwide study. Med. Mycol. (IF 2.822) Pub Date : 2020-06-23 Hélène Chaussade,Xavier Cazals,Guillaume Desoubeaux,Gregory Jouvion,Marie-Elisabeth Bougnoux,Agnes Lefort,Claire Rivoisy,Marie Desnos-Ollivier,Fabrice Chretien,Taieb Chouaki,Bérengère Gruson,Louis Bernard,Olivier Lortholary,Fanny Lanternier,
Though candidiasis is the most frequent invasive fungal infection, Candida spp. central nervous system (CNS) infections are rare but severe. To further describe clinico-patho-radiological presentations of this entity, we report a retrospective study from January 2005 to December 2018 including patients aged ≥ 28 days with proven or probable CNS candidiasis in France. Twenty-four patients were included
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Sociodemographic characteristics and spectrum of Malassezia species in individuals with and without seborrhoeic dermatitis/dandruff: A comparison of residents of the urban and rural populations. Med. Mycol. (IF 2.822) Pub Date : 2020-06-18 Prasanna Honnavar,Arunaloke Chakrabarti,Manpreet Dhaliwal,Sunil Dogra,Sanjeev Handa,P V M Lakshmi,Shivaprakash M Rudramurthy
Seborrhoeic dermatitis/dandruff (SD/D) is a common, persistent, relapsing inflammatory condition affecting the areas rich in sebaceous glands. SD/D is widely prevalent in India but Malassezia species implicated are not well studied. To estimate the prevalence and spectrum of Malassezia species causing SD/D and understand the sociodemographic characteristics of SD/D in rural and urban populations, a
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Novel antifungal activity of oligostyrylbenzenes compounds on Candida tropicalis biofilms. Med. Mycol. (IF 2.822) Pub Date : 2020-06-15 Melisa A Quinteros,Ivana L D Galera,Juan Tolosa,Joaquín C García-Martínez,Paulina L Páez,María G Paraje
As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene)vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and
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Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group. Med. Mycol. (IF 2.822) Pub Date : 2020-06-13 S Rocchi,E Scherer,C Mengoli,A Alanio,F Botterel,M E Bougnoux,S Bretagne,M Cogliati,M Cornu,F Dalle,C Damiani,J Denis,S Fuchs,M Gits-Muselli,F Hagen,C Halliday,R Hare,X Iriart,C Klaassen,M Lackner,M Lengerova,V Letscher-Bru,F Morio,C Nourrisson,W Posch,B Sendid,J Springer,B Willinger,P L White,R A Barnes,M Cruciani,J P Donnelly,J Loeffler,L Millon
Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different
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Functional analysis of two STAT1 gain-of-function mutations in two Iranian families with autosomal dominant chronic mucocutaneous candidiasis. Med. Mycol. (IF 2.822) Pub Date : 2020-06-11 Vajiheh Ostadi,Roya Sherkat,Melanie Migaud,Seyed-Mehran Modaressadeghi,Jean-Laurent Casanova,Anne Puel,Nioosha Nekooie-Marnany,Mazdak Ganjalikhani-Hakemi
Candidiasis is characterized by susceptibility to recurrent or persistent infections caused by Candida spp., typically Candida albicans, of cutaneous and mucosal surfaces. In this report, function and frequency of Th17 cells as well as genetics of patients susceptible to mucocutaneous candidiasis were studied. For patients, T-cell proliferation tests in response to Candida antigen, Th17 cell proportions
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CHROMagarTM Candida Plus: A novel chromogenic agar that permits the rapid identification of Candida auris. Med. Mycol. (IF 2.822) Pub Date : 2020-06-11 Andrew M Borman,Mark Fraser,Elizabeth M Johnson
Candida auris is a serious nosocomial health risk, with widespread outbreaks in hospitals worldwide. Successful management of such outbreaks has depended upon intensive screening of patients to identify those that are colonized and the subsequent isolation or cohorting of affected patients to prevent onward transmission. Here we describe the evaluation of a novel chromogenic agar, CHROMagarTM Candida
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New multilocus sequence typing primers to enable genotyping of AD hybrids within the Cryptococcus neoformans species complex. Med. Mycol. (IF 2.822) Pub Date : 2020-06-08 Massimo Cogliati,Frederic Roger,Wieland Meyer,Vincent Robert,Sébastien Bertout
Abstract Although AD hybrids within the Cryptococcus neoformans species complex represent about 20% of the isolates identified in Europe, phylogenetic and population genetic studies are lacking due to the inability to use the standardized typing method. The aim of the present study was to design new molecular type specific primers in order to apply the standard ISHAM consensus multilocus sequence
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A case-series of bloodstream infections caused by the Meyerozyma guilliermondii species complex at a reference center of oncology in Brazil. Med. Mycol. (IF 2.822) Pub Date : 2020-06-04 Alessandra Leal Silva Chaves,Luciana Trilles,Gabriela Machado Alves,Maria Helena Galdino Figueiredo-Carvalho,Fábio Brito-Santos,Rowena Alves Coelho,Ianick S Martins,Rodrigo Almeida-Paes
Bloodstream infections (BSI) caused by Candida species are the fourth cause of healthcare associated infections worldwide. Non-albicans Candida species emerged in the last decades as agents of serious diseases. In this study, clinical and microbiological aspects of six patients with BSI due to the Meyerozyma (Candida) guilliermondii species complex from an oncology reference center in Brazil, were
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Low rate of azole resistance in cases of avian aspergillosis in Germany. Med. Mycol. (IF 2.822) Pub Date : 2020-06-04 Amelia E Barber,Sandra Scheufen,Grit Walther,Oliver Kurzai,Volker Schmidt
Aspergillosis is the most common fungal disease of the avian respiratory tract. Due to delayed diagnosis and treatment failure, the outcome of these infections is often poor. We investigate 159 cases of avian aspergillosis among captive birds in Germany to define clinical features as well as the frequency of in vitro triazole resistance. Adult birds were more likely to present with clinical signs compared
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Pneumocystis jirovecii and microsporidia: An unusual coinfection in HIV patients? Med. Mycol. (IF 2.822) Pub Date : 2020-06-04 Yaxsier de Armas,Virginia Capó,Fernando Jorge Bornay-Linares,Carmen Del Águila,Olga Matos,Enrique J Calderón
Pneumocystis jirovecii and microsporidia species are recognized as opportunistic infectious pathogens in AIDS patients. Coinfection of both in one patient has been rarely reported. The aim of the present study was to investigate the coinfection of P. jirovecii and microsporidia in different tissues from AIDS deceased patients. Post mortem histological finding of P. jirovecii and microsporidia was demonstrated
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The novel fungal CYP51 inhibitor VT-1598 displays classic dose-dependent antifungal activity in murine models of invasive aspergillosis. Med. Mycol. (IF 2.822) Pub Date : 2020-06-01 E P Garvey,A D Sharp,P A Warn,C M Yates,M Atari,S Thomas,R J Schotzinger
Aspergillus spp. infections remain a global concern, with ∼30% attributable mortality of invasive aspergillosis (IA). VT-1598 is a novel fungal CYP51 inhibitor designed for exquisite selectivity versus human CYP enzymes to achieve a maximal therapeutic index and therefore maximal antifungal efficacy. Previously, its broad-spectrum in vitro antifungal activity was reported. We report here the pharmacokinetics
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Challenging diagnosis of chronic cerebral fungal infection: Value of (1→3)-ß-D-glucan and mannan antigen testing in cerebrospinal fluid and of cerebral ventricle puncture. Med. Mycol. (IF 2.822) Pub Date : 2020-05-29 Claire A Hobson,Guillaume Desoubeaux,Claudia Carvalho-Schneider,Christophe Destrieux,Jean-Philippe Cottier,Denis Garot,Cécile Le Brun,Zoha Maakaroun,Adrien Lemaignen,Éric Bailly,Louis Bernard
AbstractPrimary fungal infection of the central nervous system (CNS) is rare but often associated with severe prognosis. Diagnosis is complicated since cerebrospinal fluid (CSF) samples obtained from lumbar puncture usually remain sterile. Testing for fungal antigens in CSF could be a complementary diagnostic tool. We conducted such measurements in CSF from patients with CNS fungal infection and now
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Central nervous system blastomycosis clinical characteristics and outcomes. Med. Mycol. (IF 2.822) Pub Date : 2020-05-29 Kathryn Majdick,Keaton Kaye,Mahmoud A Shorman
AbstractBlastomycosis is a local or systemic infection, caused by Blastomyces dermatitidis (B. dermatitidis) or B. gilchristii. Blastomycosis has been described as “the great pretender,” alluding to the fact that it manifests in a wide range of symptoms and disease severity. Central nervous system (CNS) involvement, although rare, carries significant mortality. Due to the limited published reports
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Stimulation of surfactant exocytosis in primary alveolar type II cells by A. fumigatus. Med. Mycol. (IF 2.822) Pub Date : 2020-05-27 Natalia Schiefermeier-Mach,Susanne Perkhofer,Lea Heinrich,Thomas Haller
Aspergillus fumigatus is an opportunistic fungal pathogen with small airborne spores (conidia) that may escape clearance by upper airways and directly impact the alveolar epithelium. Consequently, innate alveolar defense mechanisms are being activated, including professional phagocytosis by alveolar macrophages, recruitment of circulating neutrophils and probably enhanced secretion of pulmonary surfactant
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Paracoccidioides brasiliensis activates mesenchymal stem cells through TLR2, TLR4, and Dectin-1. Med. Mycol. (IF 2.822) Pub Date : 2020-05-27 Carolina Rodriguez-Echeverri,Juan David Puerta-Arias,Ángel González
Numerous researchers have described the potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) for the treatment of various infectious and inflammatory diseases. However, contrary to what has been reported, the transplantation of BM-MSCs in a mouse model of Paracoccidioides brasiliensis-induced pulmonary fibrosis exacerbated the inflammatory process and fibrosis, worsening the course of
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Abundance interaction in Candida albicans and Candida glabrata mixed biofilms under diverse conditions. Med. Mycol. (IF 2.822) Pub Date : 2020-05-26 Qianqian Li,Juanjuan Liu,Mengli Chen,Kelong Ma,Tianming Wang,Daqiang Wu,Guiming Yan,Changzhong Wang,Jing Shao
Candida albicans and Candida glabrata are frequently coisolated from the oral cavity in immunosuppressive or immunocompromised individuals. Their relationship is usually defined as competition as C. glabrata can inhibit growth of C. albicans in cohabitation. In this study, eight C. albicans isolates as well as two C. glabrata strains were used to investigate the effects of culture medium (Roswell Park
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Comparative accuracy of 1,3 beta-D glucan and galactomannan for diagnosis of invasive fungal infections in pediatric patients: a systematic review with meta-analysis. Med. Mycol. (IF 2.822) Pub Date : 2020-05-25 Shreya Singh,Manvi Singh,Nipun Verma,Minakshi Sharma,Pranita Pradhan,Anil Chauhan,Nishant Jaiswal,Arunaloke Chakrabarti,Meenu Singh
Invasive fungal infections (IFI) cause considerable morbidity and mortality in pediatric patients. Serum biomarkers such as 1,3-beta-D glucan (BDG) and galactomannan (GM) have been evaluated for the IFI diagnosis. However, most evidence regarding their utility is derived from studies in adult oncology patients. This systematic review aimed to compare the diagnostic accuracy of BDG and GM individually
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Multicenter evaluation of three different MALDI-TOF MS systems for identification of clinically relevant filamentous fungi. Med. Mycol. (IF 2.822) Pub Date : 2020-05-21 Yue Sun,Jian Guo,Rong Chen,Liang Hu,Qihang Xia,Wenjuan Wu,Jinghua Wang,Fupin Hu
AbstractMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) holds promise as a potential tool for clinical identification of filamentous fungi. However, due to the lack of an appropriate extraction protocol and the difficulty of database building, the identification power of each system differs. In this study, we selected 126 clinical mould isolates comprising
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Adjunctive sertraline for asymptomatic cryptococcal antigenemia: A randomized clinical trial. Med. Mycol. (IF 2.822) Pub Date : 2020-05-16 David R Boulware,Elizabeth Nalintya,Radha Rajasingham,Paul Kirumira,Rose Naluyima,Fred Turya,Sylvia Namanda,Morris K Rutakingirwa,Caleb P Skipper,Yofesi Nikweri,Kathy Huppler Hullsiek,Ananta S Bangdiwala,David B Meya
Cryptococcal antigen (CrAg) screening in HIV-infected persons with CD4 < 100 cells/µl can reduce meningitis and death, yet preemptive fluconazole therapy fails in ∼25%. Sertraline has in vitro and in vivo activity against Cryptococcus and is synergistic with fluconazole in mice. We evaluated the efficacy and safety of sertraline in asymptomatic cryptococcal antigenemia. We conducted a randomized trial
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Analysis of biofilm formation by Sporothrix schenckii. Med. Mycol. (IF 2.822) Pub Date : 2020-05-14 Rocío Sánchez-Herrera,Lérida Liss Flores-Villavicencio,Juan Luis Pichardo-Molina,José Pedro Castruita-Domínguez,Xochilt Aparicio-Fernández,Myrna Sabanero López,Julio Cesar Villagómez-Castro
AbstractThe development of mature biofilms is an aid in numerous aspects of the life cycle of fungi. It is well known that Sporothrix schenckii complex causes a benign subcutaneous mycosis, but recent studies have suggestedthat biofilm formation may be one of the important factors involved in its virulence. Here we report the study of the biomass organization and a model of the stages of S. schenckii
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In vitro pharmacokinetics/pharmacodynamics modeling and efficacy against systemic candidiasis in Drosophila melanogaster of a bisaryloxypropanamine derivative. Med. Mycol. (IF 2.822) Pub Date : 2020-05-14 Daiane Flores Dalla Lana,Taís Fernanda Andrzejewski Kaminski,Stefânia Neiva Lavorato,Simone Merkel,Régis Adriel Zanette,Priscila Dallé da Rosa,Keli Jaqueline Staudt,Bibiana Verlindo de Araújo,Bárbara da Costa,Priscilla Maciel Quatrin,Luana Candice Genz Bazana,Felipe Alves Ferreira,Cássia Ferreira Braz Caurio,Saulo Fernandes de Andrade,Ricardo José Alves,Alexandre Meneghello Fuentefria
AbstractThe number of deaths due to systemic fungal infections is increasing alarmingly, which is aggravated by the limitations of traditional treatments and multidrug resistance. Therefore, the research and development of new therapeutic options against pathogenic fungi is an urgent need. To evaluate the fungicidal activity of a synthetic compound, 1,3-bis-(3,4-dichlorophenoxy)propan-2-aminium chloride
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Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches. Med. Mycol. (IF 2.822) Pub Date : 2020-05-13 Marjorie Bateman,Rita Oladele,Jay K Kolls
Pneumocystis jirovecii can cause life-threatening pneumonia in immunocompromised patients. Traditional diagnostic testing has relied on staining and direct visualization of the life-forms in bronchoalveolar lavage fluid. This method has proven insensitive, and invasive procedures may be needed to obtain adequate samples. Molecular methods of detection such as polymerase chain reaction (PCR), loop-mediated
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Performance characteristics of Fungitell STAT™, a rapid (1→3)-β-D-glucan single patient sample in vitro diagnostic assay. Med. Mycol. (IF 2.822) Pub Date : 2020-05-13 Robert L D'Ordine,Kevin A Garcia,Josee Roy,Yonglong Zhang,Barbara Markley,Malcolm A Finkelman
AbstractSerum (1→3)-β-D-glucan (BDG), is an adjunct test in the diagnosis of invasive fungal disease (IFD). Fungitell STAT™, a facile, rapid, single patient option, executable for one or more patient specimens in approximately an hour, has been developed to address a need for rapid in-house testing. This method presents qualitative information concerning serum BDG levels, using an index value that
更新日期:2020-05-13