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The influence of the phylum Planctomycetota in the environmental resistome Res. Microbiol. (IF 2.6) Pub Date : 2024-03-11 Ofélia Godinho, Damien P. Devos, Sandra Quinteira, Olga M. Lage
Antimicrobial resistance is one of the leading causes of death worldwide and research on this topic has been on the spotlight for a long time. More recently and in agreement with the One Health Approach, the focus has moved towards the environmental resistome. Members of the phylum are ubiquitously present in the environment including in hotspots for antimicrobial resistance selection and dissemination
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Biochemical characterization and mutational analysis of the NurA protein from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5 Res. Microbiol. (IF 2.6) Pub Date : 2024-02-24 Guangyu Ma, Tan Lin, Peng Cao, Philippe Oger, Kunming Dong, Li Miao, Likui Zhang
Archaeal NurA protein plays a key role in producing 3′-single stranded DNA used for homologous recombination repair, together with HerA, Mre11, and Rad50. Herein, we describe biochemical characteristics and roles of key amino acid residues of the NurA protein from the hyperthermophilic euryarchaeon Ch5 (Tba-NurA). Tba-NurA possesses 5′–3′ exonuclease activity for degrading DNA, displaying maximum efficiency
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The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis Res. Microbiol. (IF 2.6) Pub Date : 2024-01-28 Feng Peng, Yu Zou, Xiuxia Liu, Yankun Yang, Jing Chen, Jianqi Nie, Danni Huang, Zhonghu Bai
The complete genome of C. glutamicum contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that mepA overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-mCherry was shown to localizes
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Effects of ultraviolet and photosynthetically active radiation on morphogenesis, antioxidants and photoprotective defense mechanism in a hot-spring cyanobacterium Nostoc sp. strain VKB02 Res. Microbiol. (IF 2.6) Pub Date : 2024-01-08 Nasreen Amin, Rajeshwar P. Sinha, Vinod K. Kannaujiya
The continuous increase in global temperature and ultraviolet radiation (UVR) causes profound impacts on the growth and physiology of photosynthetic microorganisms. The hot-spring cyanobacteria have a wide range of mitigation mechanisms to cope up against current unsustainable environmental conditions. In the present investigation, we have explored the indispensable mitigation strategies of an isolated
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Elevated concentrations of polymyxin B elicit a biofilm-specific resistance mechanism in Vibrio cholerae Res. Microbiol. (IF 2.6) Pub Date : 2024-01-05 Julien Pauzé-Foixet, Annabelle Mathieu-Denoncourt, Marylise Duperthuy
Vibrio cholerae can form biofilms in the aquatic environment and in the human intestine, facilitating the release of hyper-infectious aggregates. Due to the increasing antibiotic resistance, alternatives need to be found. One of these alternatives is antimicrobial peptides, including polymyxin B (PmB). In this study, we first investigated the resistance of V. cholerae O1 El Tor strain A1552 to various
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The role of intracellular β-glucosidase in cellulolytic response induction in filamentous fungus Penicillium verruculosum Res. Microbiol. (IF 2.6) Pub Date : 2023-12-30 Valeriy Yu. Kislitsin, Andrey M. Chulkin, Anna S. Dotsenko, Igor G. Sinelnikov, Arkady P. Sinitsyn, Aleksandra M. Rozhkova
In this study, CRISPR/Cas9 genome editing was used to knockout the bgl2 gene encoding intracellular β-glucosidase filamentous fungus Penicillium verruculosum. This resulted in a dramatic reduction of secretion of cellulolytic enzymes. The study of P. verruculosum Δbgl2 found that the transcription of the cbh1 gene, which encodes cellobiohydrolase 1, was impaired when induced by cellobiose and cellotriose
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Termitidicoccus mucosus gen. nov. sp. nov. a novel Verrucomicrobiota species isolated from Reticulitermes chinensis gives insights of high adaptability of symbiotic bacteria to termite gut ecosystem Res. Microbiol. (IF 2.6) Pub Date : 2023-12-28 Cheng Mei, Yu Shi, Yu Wang, Zhengyong Qiu, Hong Yang
Verrucomicrobiota is widely distributed in various habitats including insect guts. It was found to be prevalent in almost all investigated termite guts, whereas their physiological functions are not very clear. In this study we characterized the physiological and genomic properties of Verrucomicrobiota strain TSB47T isolated from Reticulitermes chinensis. The cells of strain TSB47T were Gram-stain-negative
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The stringent response is strongly activated in the antibiotic producing strain, Streptomyces coelicolor. Res. Microbiol. (IF 2.6) Pub Date : 2023-12-28 Clara Lejeune, David Cornu, Laila Sago, Virginie Redeker, Marie-Joelle Virolle
S. lividans and S. coelicolor are phylogenetically closely related strains with different abilities to produce the same specialized metabolites. Previous studies revealed that the strong antibiotic producer, S. coelicolor, had a lower ability to assimilate nitrogen and phosphate than the weak producer, S. lividans, and this resulted into a lower growth rate. A comparative proteomic dataset was used
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Review of techniques for the in-situ sterilization of soil contaminated with Bacillus anthracis spores or other pathogens Res. Microbiol. (IF 2.6) Pub Date : 2023-12-21 Joseph P. Wood
This review summarizes the literature on efficacy of techniques to sterilize soil. Soil may need to be sterilized if contaminated with pathogens such as Bacillus anthracis. Sterilizing soil in-situ minimizes spread of the bio-contaminant. Soil is difficult to sterilize, with efficacy generally diminishing with depth. Methyl bromide, formaldehyde, and glutaraldehyde are the only soil treatment options
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Prevalence, antimicrobial resistance, and genetic lineages of nasal Staphylococcus aureus among medical students at a Spanish University: Detection of the MSSA-CC398-IEC-type-C subclade Res. Microbiol. (IF 2.6) Pub Date : 2023-12-21 Nerea C. Rosales-González, Margarita González-Martín, Idris Nasir Abdullahi, MaTeresa Tejedor-Junco, Javier Latorre-Fernández
Medical students could be a potential source of Staphylococcus aureus transmission to patients. This cross-sectional study involved samples collected from both nasal nostrils. Samples were processed for S. aureus recovery; the antimicrobial resistance (AMR) phenotype was determined by disc diffusion assays and the spa types and AMR genotypes by PCR/sequencing. A structured questionnaire was administered
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Editorial: Special issue on International Biohydrometallurgy Symposium (IBS) 2022 Res. Microbiol. (IF 2.6) Pub Date : 2023-12-20 Elizabeth Watkin, Axel Schippers, Melissa Corbett
Abstract not available
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Bacterial biofilm formation and anti-biofilm strategies Res. Microbiol. (IF 2.6) Pub Date : 2023-12-02 Sesan Abiodun Aransiola, Barathi Selvaraj, Naga Raju Maddela
Abstract not available
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The dynamics of two iron-oxidizing Acidithiobacillus strains in industrial copper sulfide heap-leaching Res. Microbiol. (IF 2.6) Pub Date : 2023-11-22 Camila Escuti, Roberto Véliz, Mauricio Acosta, Alex Echeverría-Vega, Gonzalo Araya, Diego Ayma, Cecilia Demergasso
Several species within the Acidithiobacillus (At.) genus can derive energy from oxidizing ferrous iron and sulfur. Two bacterial strains according to their 16S rRNA gene sequences closely related to At. ferridurans and At. ferrivorans were obtained from the industrial sulfide heap leaching process at Minera Escondida (SLH), named D2 and DM, respectively. We applied statistical and data mining analyses
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Comprehensive updates on the biological features and metabolic potential of the versatile extremophilic actinomycete Nocardiopsis dassonvillei Res. Microbiol. (IF 2.6) Pub Date : 2023-11-21 Shivani Bhairamkar, Pratik Kadam, H. Anjulal, Avani Joshi, Riddhi Chaudhari, Dimpal Bagul, Vaishali Javdekar, Smita Zinjarde
Nocardiopsis dassonvillei prevails under harsh environmental conditions and the purpose of this review is to highlight its biological features and recent biotechnological applications. The organism prevails in salt-rich soils/marine systems and some strains endure extreme temperatures and pH. A few isolates are associated with marine organisms and others cause human diseases. Comparative genomic analysis
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Utilization of glycoprotein-derived N-acetylglucosamine-L-asparagine during Enterococcus faecalis infection depends on catabolic and transport enzymes of the glycosylasparaginase locus Res. Microbiol. (IF 2.6) Pub Date : 2023-11-16 Victor Combret, Isabelle Rincé, Aurélie Budin-Verneuil, Cécile Muller, Josef Deutscher, Axel Hartke, Nicolas Sauvageot
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Protein Kinase A Regulatory Subunit is Required for Normal Growth, Zoosporogenesis, and Pathogenicity in Phytophthora Sojae Res. Microbiol. (IF 2.6) Pub Date : 2023-11-11 Yunxiang Zhang, Zhuo Zhang, Yue Chen, Xinqiu Tan, Yong Liu, Zhe Tian, Jinglin Wang, Xin Zhang, Deyong Zhang
Phytophthora sojae, one of the most devastating Oomycete pathogens, causes severe diseases that lead to economic loss in the soybean industry. The production of zoospores play a crucial role during the development of Phytophthora disease. In this work, CRISPR/Cas9 genome editing technology were used to obtain protein kinase A regulatory subunit (PsPkaR) knockout mutants. The role of PsPkaR in the production
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Transcriptomic signature of bacteria exposed to benzalkonium chloride Res. Microbiol. (IF 2.6) Pub Date : 2023-11-10 Samantha J. McCarlie, Louis L. du Preez, Julio Castillo Hernandez, Charlotte E. Boucher, Robert R. Bragg
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Alicyclobacillus sp. SO9, a novel halophilic acidophilic iron-oxidizing bacterium isolated from a tailings-contaminated beach, and its effect on copper extraction from chalcopyrite in the presence of high chloride concentration Res. Microbiol. (IF 2.6) Pub Date : 2023-11-04 Dieu Huynh, Götz Haferburg, Boyke Bunk, Stefan R. Kaschabek, Wolfgang Sand, Michael Schlömann
Many acidophilic iron-oxidizing bacteria used in the mining industry for the bioleaching of sulfidic minerals are intolerant to high chloride concentrations, resulting in problems where chloride occurs in the deposit at high concentrations or only seawater is available. In search for strains tolerating such conditions a tetrathionate- and iron-oxidizing bacterium was isolated from a tailings-contaminated
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Biofilms communities in the soil: characteristic and interactions using mathematical model Res. Microbiol. (IF 2.6) Pub Date : 2023-11-03 Ojeba Innocent Musa, Sikirulai Abolaji Akande, Udeme Joshua Josiah Ijah, Olabisi Peter Abioye, Asmau Mohammed Maude, Job Oloruntoba Samuel, Adamu Mustapha, Al-Musbahu Abdulrahim, Alberto Campos García Gusdanis
There are many different kinds of microorganisms in the soil, and many of them are biofilms because they can make supracellular compounds. Surface-associated microorganisms in a biofilm are encased in a hydrated extracellular polymeric substance that aids in adherence and survival. Numerous different kinds of microorganisms call the soil home. Strong interactions with and among species are made possible
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Microbial anti-biofilms: types and mechanism of action Res. Microbiol. (IF 2.6) Pub Date : 2023-10-14 Ibrahim Mohammed Hussaini, Oluwafemi Adebayo Oyewole, Mamunu Abdulkadir Sulaiman, Abdullahi Idris Dabban, Asmau Nna Sulaiman, Reham Tarek
Biofilms have been recognized as a serious threat to public health as it protects microbes from antimicrobials, immune defence mechanisms, chemical treatments and nutritional stress. Biofilms are also a source of concern in industries and water treatment because their presence compromises the integrity of equipment. To overcome these problems, it is necessary to identify novel anti-biofilm compounds
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Microbial immobilisation and adaptation to Cu2+ enhances microbial Fe2+ oxidation for bioleaching of printed circuit boards in the presence of mixed metal ions Res. Microbiol. (IF 2.6) Pub Date : 2023-10-07 Musa D. Maluleke, Athanasios Kotsiopoulos, Elaine Govender-Opitz, Susan T.L. Harrison
A circular economy requires effective re-use of finite resources, such as metals from waste electrical and electronic equipment (WEEE). Bioleaching for extraction and recovery of base metals from printed circuit boards (PCBs) before recovering precious metals has potential to increase metal circularity. However, inhibition by base metals released from the PCBs and accumulated in PCB leachates on microbial
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Sulfur oxidation kinetics of Acidithiobacillus caldus and its inhibition on exposure to thiocyanate present in cyanidation tailings wastewater Res. Microbiol. (IF 2.6) Pub Date : 2023-09-28 Catherine J. Edward, Mariette Smart, Athanasios Kotsiopoulos, Susan T.L. Harrison
The sulfur oxidation kinetics of an industrial strain of Acidithiobacillus caldus (At. caldus) cultured on elemental sulfur was explored in batch experiments in the absence and presence of thiocyanate (SCN−), a toxin inherent within cyanidation tailings wastewater. The Contois rate expression accurately described At. caldus sulfate generation (R2 > 0.93) and microbial growth (R2 > 0.87). For a culture
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The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus Res. Microbiol. (IF 2.6) Pub Date : 2023-09-25 Michael Gorgan, Shahar Vanunu Ofri, Emilee R. Engler, Avishag Yehuda, Elizabeth Hutnick, Zvi Hayouka, Michael A. Bertucci
The opportunistic human pathogen Bacillus cereus controls the expression of key infection-promoting phenotypes using bacterial quorum sensing (QS). QS signal transduction within the species is controlled by an autoinducing peptide, PapR7, and its cognate receptor, PlcR, indicating that the PlcR:PapR interface is a prime target for QS inhibitor development. The C-terminal region of the peptide (PapR7;
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Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage Res. Microbiol. (IF 2.6) Pub Date : 2023-09-16 Nurit Harel, Navit Ogen-Shtern, Leah Reshef, Dvora Biran, Eliora Z. Ron, Uri Gophna
Sun exposure is harmful to the skin and increases the risk of skin aging and skin cancer. Here we examined the effects of daily exposure to sun radiation on the skin microbiome in order to determine whether skim microbiome bacteria can contribute to protection from solar damage. Skin swabs were collected from ten lifeguards before and after the summer to analyse the skin microbiome. The results indicate
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Programmed cell death in Xanthomonas axonopodis pv. glycines is associated with modulation of gene expression resulting in altered states of motility, biofilm and virulence Res. Microbiol. (IF 2.6) Pub Date : 2023-09-14 Nilantana C. Bandyopadhyay, Satyendra Gautam
One of the foremost report of apoptosis-like programmed cell death (PCD) came from Xanthomonas axonopodis pv. glycines (Xag), which displayed rapid post-exponential cell death in PCD inducing media (PIM) but not in a non-inducing media (PNIM). The current study aims to decipher for the first time, the advantages of the existence of PCD in this phytopathogenic microorganism. Analysis of RNA-seq under
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Comparative genomics sheds light on transcription factor-mediated regulation in the extreme acidophilic Acidithiobacillia representatives Res. Microbiol. (IF 2.6) Pub Date : 2023-09-09 Pedro Sepúlveda-Rebolledo, Carolina González-Rosales, Mark Dopson, Ernesto Pérez-Rueda, David S. Holmes, Jorge H. Valdés
Extreme acidophiles thrive in acidic environments, confront a multitude of challenges, and demonstrate remarkable adaptability in their metabolism to cope with the ever-changing environmental fluctuations, which encompass variations in temperature, pH levels, and the availability of electron acceptors and donors. The survival and proliferation of members within the Acidithiobacillia class rely on the
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trans-translation system is important for maintaining genome integrity during DNA damage in bacteria Res. Microbiol. (IF 2.6) Pub Date : 2023-09-09 Nagarajan T, Sutharsan Govindarajan, M. Hussain Munavar
DNA integrity in bacteria is regulated by various factors that act on the DNA. trans-translation has previously been shown to be important for the survival of Escherichia coli cells exposed to certain DNA-damaging agents. However, the mechanisms underlying this sensitivity are poorly understood. In this study, we explored the involvement of the trans-translation system in the maintenance of genome
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Analysis of element yield, bacterial community structure and the impact of carbon sources for bioleaching rare earth elements from high grade monazite Res. Microbiol. (IF 2.6) Pub Date : 2023-09-06 Melissa K. Corbett, April Gifford, Nick Fimognari, Elizabeth L.J. Watkin
Rare earth element (REE) recovery from waste streams, mine tailings or recyclable components using bioleaching is gaining traction due to the shortage and security of REE supply as well as the environmental problems that occur from processing and refining. Four heterotrophic microbial species with known phosphate solubilizing capabilities were evaluated for their ability to leach REE from a high-grade
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Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response Res. Microbiol. (IF 2.6) Pub Date : 2023-09-01 M. Kaur, J.M. Buyck, F. Goormaghtigh, J.-L. Decout, N. Mozaheb, M.-P. Mingeot-Leclercq
Pseudomonas aeruginosa, a Gram-negative bacterium that causes severe hospital acquired infections poses threat by its ability for adaptation to various growth modes and environmental conditions and by its intrinsic resistance to antibiotics. The latter is mainly due to the outer membrane (OM) asymmetry which is maintained by the Mla pathway resulting in the retrograde transport of glycerophospholipids
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Dethiobiotin uptake and utilization by bacteria possessing bioYB operon Res. Microbiol. (IF 2.6) Pub Date : 2023-08-26 Tomoki Ikeda, Tetsuhiro Ogawa, Toshihiro Aono
Biotin is an essential vitamin for all organisms. Some bacteria cannot synthesize biotin and live by acquiring biotin from the environment. Bacterial biotin transporters (BioY) are classified into three mechanistic types. The first forms the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). The second relies on a promiscuous energy coupling module. The third functions independently
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Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: a resource for fungal biology and biotechnology Res. Microbiol. (IF 2.6) Pub Date : 2023-08-11 Carlos Godinho de Abreu, Luiz Fernando Wurdig Roesch, Fernando Dini Andreote, Saura Rodrigues Silva, Tatiana Silveira Junqueira de Moraes, Diego Cunha Zied, Félix Gonçalves de Siqueira, Eustáquio Souza Dias, Alessandro M. Varani, Victor Satler Pylro
Agaricus subrufescens, also known as the “sun mushroom,” has significant nutritional and medicinal value. However, its short shelf life due to the browning process results in post-harvest losses unless it's quickly dehydrated. This restricts its availability to consumers in the form of capsules. A genome sequence of A. subrufescens may lead to new cultivation alternatives or the application of gene
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Simulating compatible solute biosynthesis using a metabolic flux model of the biomining acidophile, Acidithiobacillus ferrooxidans ATCC 23270 Res. Microbiol. (IF 2.6) Pub Date : 2023-08-10 Himel Nahreen Khaleque, Hadi Nazem-Bokaee, Yosephine Gumulya, Ross P. Carlson, Anna H. Kaksonen
Halotolerant, acidophilic, bioleaching microorganisms are crucial to biomining operations that utilize saline water. Compatible solutes play an important role in the adaptation of these microorganisms to saline environments. Acidithiobacillus ferrooxidans ATCC 23270, an iron- and sulfur-oxidizing acidophilic bacterium, synthesizes trehalose as its native compatible solute but is still sensitive to
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Corynebacterium ramonii sp. nov., a novel toxigenic member of the Corynebacterium diphtheriae species complex Res. Microbiol. (IF 2.6) Pub Date : 2023-08-10 Chiara Crestani, Gabriele Arcari, Annie Landier, Virginie Passet, Dorian Garnier, Sylvie Brémont, Nathalie Armatys, Annick Carmi-Leroy, Julie Toubiana, Edgar Badell, Sylvain Brisse
The Corynebacterium diphtheriae species complex comprises seven bacterial species, including Corynebacterium ulcerans, a zoonotic pathogen from multiple animal species. In this work, we characterise phenotypically and genotypically isolates belonging to two C. ulcerans lineages. Results from phylogenetic analyses, in silico DNA–DNA hybridization (DDH) and MALDI-TOF spectra differentiate lineage 2 from
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IS257-mediated amplification of tet(L) variant as a novel mechanism of enhanced tigecycline resistance in Staphylococcus cohnii Res. Microbiol. (IF 2.6) Pub Date : 2023-08-10 Hong Yao, Hongjie Xing, Nannan Wang, Likuan Zhang, Stefan Schwarz, Chenglong Li, Chang Cai, Chunyan Xu, Xiang-Dang Du
The mechanism of enhanced tigecycline MIC in Staphylococcus cohnii after in vitro tigecycline exposure was investigated. S. cohnii 11-B-312 was exposed to incremental concentrations of tigecycline (2–32 mg/L) and the mutants growing at 8, 16 and 32 mg/L were determined by AST and WGS. Copy number and relative transcription level of the tet(L) gene were determined by quantitative PCR. The fitness cost
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Bioleaching of polymetallic sulphidic mining residues: influence of increasing solid concentration on microbial community dynamics and metal dissolution Res. Microbiol. (IF 2.6) Pub Date : 2023-08-06 Catherine Joulian, Agathe Hubau, Douglas Pino-Herrera, Anne-Gwénaëlle Guezennec
Within the European research project NEMO, a bioleaching strategy was developed for efficient metal extraction from bioleach residue currently heap-leached at Sotkamo (Finland) that still contains sulphidic minerals and valuable metals (Ni, Zn, Co, Cu). The strategy of gradually increasing the solid content with 5% steps allowed the adaptation of the consortium up to 20% (w/w) solid content, with efficient
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Rates of iron(III) reduction coupled to elemental sulfur or tetrathionate oxidation by acidophilic microorganisms and detection of sulfur intermediates Res. Microbiol. (IF 2.6) Pub Date : 2023-08-04 Anja Breuker, Axel Schippers
Bioleaching processes and acid mine drainage (AMD) generation are mainly driven by aerobic microbial iron(II) and inorganic sulfur/compound oxidation. Dissimilatory iron(III) reduction coupled to sulfur/compound oxidation (DIRSO) by acidophilic microorganisms has been described for anaerobic cultures, but iron reduction was observed under aerobic conditions as well. Aim of this study was to explore
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Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species Res. Microbiol. (IF 2.6) Pub Date : 2023-07-28 Abhijit Sarma, Gunasekaran Dhandapani, Homen Phukan, Prasun Kumar Bhunia, Arun Kumar De, Debasis Bhattacharya, T. Jebasingh, Madathiparambil G. Madanan
Leptospirosis, a global reemerging zoonosis caused by the spirochete Leptospira, has severe human and veterinary implications. Cell wall hydrolase (LIC_10271) with LytM (peptidase M23) and LysM domains are found to be associated with various pathogenic bacteria. These domains regulate effects on extracellular matrix and biofilm components, which promote cell wall remodeling and pathogen dissemination
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Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress Res. Microbiol. (IF 2.6) Pub Date : 2023-07-28 Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao
The practical significance of constructing robust industrial production strains against organic acid stress lies not only in improving fermentation efficiency but also in reducing manufacturing costs. In a previous study, we constructed an industrial Saccharomyces cerevisiae strain by modifying another PEP4-allele of a mutant that already had one PEP4-allele disrupted. This modification enhanced cellular
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Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation Res. Microbiol. (IF 2.6) Pub Date : 2023-07-27 Arghya Bhowmick, Koustav Bhakta, Mousam Roy, Sayandeep Gupta, Jagriti Das, Shirsha Samanta, Somi Patranabis, Abhrajyoti Ghosh
Sulfolobus acidocaldarius, a thermoacidophilic crenarchaeon, frequently encounters temperature fluctuations, oxidative stress, and nutrient limitations in its environment. Here, we employed a high-throughput transcriptomic analysis to examine how the gene expression of S. acidocaldarius changes when exposed to high temperatures (92 °C). The data obtained was subsequently validated using quantitative
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Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli Res. Microbiol. (IF 2.6) Pub Date : 2023-07-27 Galina V. Smirnova, Aleksey V. Tyulenev, Kseniya V. Bezmaternykh, Nadezda G. Muzyka, Vadim Y. Ushakov, Oleg N. Oktyabrsky
Metabolic rearrangements that occur during depletion of essential nutrients can lead to accumulation of potentially dangerous metabolites. Here we showed that depletion of phosphate (Pi), accompanied by a sharp inhibition of growth and respiration, caused a transient excess of intracellular cysteine due to a decrease in the rate of protein synthesis. High cysteine level can be dangerous due to its
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Assessment of the growth inhibition and anti-biofilm activity of aptamer (PmA2G02) against Proteus mirabilis 1429T Res. Microbiol. (IF 2.6) Pub Date : 2023-07-08 Rajalakshmi E, Archana Vishwakarma, Anandkumar Balakrishnan, Ramya Mohandass
Proteus mirabilis is known to cause Catheter-associated urinary tract infections (CAUTIs), which exhibit virulence factors linked to forming biofilms. Aptamers have recently been explored as potential anti-biofilm agents. This study demonstrates the anti-biofilm activity of aptamer (PmA2G02) targeting Proteus mirabilis 1429T, a pathogenic bacteria known to cause Catheter-associated urinary tract infections
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Evaluating the efficacy of endolysins and membrane permeabilizers against Vibrio parahaemolyticus in marine conditions Res. Microbiol. (IF 2.6) Pub Date : 2023-07-06 Lina Angélica Zermeño-Cervantes, Sergio Francisco Martínez-Díaz, Alberto Antony Venancio-Landeros, César Salvador Cardona-Félix
Endolysins have garnered significant attention as a potential alternative to antibiotics in aquaculture, mainly for combating Vibrio spp., Gram-negative pathogens responsible for infectious outbreaks. However, endolysin effectiveness against Gram-negative bacteria is limited due to the outer membrane's poor permeability. The combat against marine pathogens poses an additional challenge of finding endolysins
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Insights into the Bacillus anthracis, cereus and thuringiensis world through the BACT conference Res. Microbiol. (IF 2.6) Pub Date : 2023-07-01 V. Broussolle, M. Gohar, L. Slamti, Jean-Nicolas Tournier
Abstract not available
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DnaJ, a heat shock protein 40 family member, is essential for the survival and virulence of plant pathogenic Pseudomonas cichorii JBC1 Res. Microbiol. (IF 2.6) Pub Date : 2023-06-24 Anh Tran Thi Ngoc, Khanh Nguyen Van, Yong Hoon Lee
Bacterial plant pathogens must cope with various environmental conditions and defenses from their hosts for colonization and infection. Heat shock proteins (HSPs) play critical roles in a variety of cellular processes, such as the maintenance of cellular homeostasis in response to environmental stress. However, the significance of HSP40 family protein DnaJ in virulence of plant pathogenic bacteria
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Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity Res. Microbiol. (IF 2.6) Pub Date : 2023-06-24 Fengru Deng, Yunsheng Chen, Huiying Xiao, Chuying Yao, Jiaqi Chen, Yuanting Zhao, Yapei Jiang, Jiahang Chen, Yiqun Deng, Jikai Wen
Probiotic products containing living microorganisms are gaining popularity, increasing the importance of their taxonomic status. A Bacillus-like isolate, 70 b, cultured from a probiotic feed additive, was ambiguity in taxonomic assignment and could be a novel member of Bacillus cereus group. The results of colony and cellular morphology, physiological and biochemical analysis mainly including growth
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Biofilm formation by selected microbial strains isolated from wastewater and their consortia: mercury resistance and removal potential Res. Microbiol. (IF 2.6) Pub Date : 2023-06-16 Ivana Radojević, Violeta Jakovljević, Sandra Grujić, Aleksandar Ostojić, Katarina Ćirković
Wastewater often contains an increased amount of mercury and, at the same time, resistant microorganisms. During wastewater treatment, a biofilm of indigenous microorganisms is often unavoidable. Therefore, the objective of this research is to isolate and identify microorganisms from wastewater and investigate their ability to form biofilms for possible application in mercury removal processes. The
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Quercetin's antibiofilm effectiveness against drug resistant Staphylococcus aureus and its validation by in silico modeling Res. Microbiol. (IF 2.6) Pub Date : 2023-06-16 Anjaneyulu Musini, Himanshu Narayan Singh, Jhansi Vulise, S. S. Sravanthi Pammi
Staphylococcus aureus is typically treated with antibiotics, however, due to its widespread and unselective usage, resistant strains of S. aureus have increased to a great extent. Treatment failure and recurring staphylococcal infections are also brought on by biofilm development, which boosts an organism's ability to withstand antibiotics and is thought to be a virulence factor in patients. The present
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Shedding light on the electron transfer chain of a moderately acidophilic iron oxidizer: characterization of recombinant HiPIP-41, CytC-18 and CytC-78 derived from Ferrovum sp. PN-J47-F6 Res. Microbiol. (IF 2.6) Pub Date : 2023-06-20 Sophie R. Ullrich, Helena Fuchs, Michael Schlömann
Efficient electron transfer from the donor to the acceptor couple presents a necessary requirement for acidophilic and neutrophilic iron oxidizers due to the low energy yield of aerobic ferrous iron oxidation. Involved periplasmic electron carriers are very diverse in these bacteria and show adaptations to the respective thermodynamic constraints such as a more positive redox potential reported for
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The NF-κB factor Relish is essential for the epithelial defenses protecting against δ-endotoxin dependent effects of Bacillus thuringiensis israelensis infection in the Drosophila model Res. Microbiol. (IF 2.6) Pub Date : 2023-06-20 Carine Mouawad, Mireille Kallassy Awad, Samuel Liegeois, Dominique Ferrandon, Vincent Sanchis-Borja, Laure El Chamy
Bacillus thuringiensis israelensis is largely regarded as the most selective, safe and ecofriendly biopesticide used for the control of insect vectors of human diseases. Bti enthomopathogenicity relies on the Cry and Cyt δ-endotoxins, produced as crystalline inclusions during sporulation. Insecticidal selectivity of Bti is mainly ascribed to the binding of the Cry toxins to receptors in the gut of
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Evolution of YacG to safeguard DNA gyrase from external perturbation Res. Microbiol. (IF 2.6) Pub Date : 2023-06-19 Priti Biswas, Sugopa Sengupta, Valakunja Nagaraja
Cells have evolved strategies to safeguard their genome integrity. We describe a mechanism to counter double strand breaks in the chromosome that involves the protection of an essential housekeeping enzyme from external agents. YacG is a DNA gyrase inhibitory protein from Escherichia coli that protects the bacterium from the cytotoxic effects of catalytic inhibitors as well as cleavage-complex stabilizers
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Beyond membrane components: uncovering the intriguing world of fungal sphingolipid synthesis and regulation Res. Microbiol. (IF 2.6) Pub Date : 2023-06-14 Sana Akhtar Usmani, Mohit Kumar, Khushboo Arya, Basharat Ali, Nitin Bhardwaj, Naseem Akhtar Gaur, Rajendra Prasad, Ashutosh Singh
Sphingolipids (SLs) are essential to fungal survival and represent a major class of structural and signaling lipids. Unique SL structures and their biosynthetic enzymes in filamentous fungi make them an ideal drug target. Several studies have contributed towards the functional characterization of specific SL metabolism genes, which have been complemented by advanced lipidomics methods which allow accurate
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The genomic approach of antimicrobial resistance of Salmonella Typhimurium isolates from guinea pigs in Lima, Peru Res. Microbiol. (IF 2.6) Pub Date : 2023-06-10 Raquel Hurtado, Debmalya Barh, Luís Cláudio Lima de Jesus, Marcus Vinicius Canário Viana, Sandeep Tiwari, Flávia Figueira Aburjaile, Dennis E. Carhuaricra Huaman, Bertram Brenig, Lenin Maturrano Hernández, Vasco Azevedo
Salmonella Typhimurium is an important agent of foodborne diseases. In Peru, the emergence of multidrug-resistant isolates of S. Typhimurium from the food chain could be linked to guinea pig farming as a potential reservoir and their uncontrolled antibiotic treatment against salmonellosis. In this study, we performed the sequencing, genomic diversity, and characterization of resistance elements transmitted
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Quenching and Quorum Sensing in Bacterial Bio-films Res. Microbiol. (IF 2.6) Pub Date : 2023-06-01 Srinivasan Kameswaran, Bellemkonda Ramesh
Quorum sensing (QS) is the ability of bacteria to monitor their population density and adjust gene expression accordingly. QS-regulated processes include host-microbe interactions, horizontal gene transfer, and multicellular behaviours (such as the growth and development of biofilm). The creation, transfer, and perception of bacterial chemicals known as autoinducers or QS signals are necessary for
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Bacteriophages potentiate the effect of antibiotics by eradication of persister cells and killing of biofilm-forming cells Res. Microbiol. (IF 2.6) Pub Date : 2023-05-30 Javiera Vera-Mansilla, Cecilia A. Silva-Valenzuela, Patricio Sánchez, Roberto C. Molina-Quiroz
Persister cells and biofilms are associated with chronic urinary infections which are more critical when generated by multi-drug resistant bacteria. In this context, joint administration of phages and antibiotics has been proposed as an alternative approach, since it may decrease the probability to generate resistant mutants to both agents. In this work, we exposed cultures of uropathogenic Escherichia