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Emerging Molecular Tools for Engineering Phytomicrobiome Indian J. Microbiol. (IF 1.83) Pub Date : 2021-01-18 Twinkle Chaudhary, Rajesh Gera, Pratyoosh Shukla1
Microbial plant interaction plays a major role in the sustainability of plants. The understanding of phytomicrobiome interactions enables the gene-editing tools for the construction of the microbial consortia. In this interaction, microbes share several common secondary metabolites and terpenoid metabolic pathways with their host plants that ensure a direct connection between the microbiome and associated
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Lactococcus lactis subsp. cremoris of Plant Origin Produces Antifungal Cyclo-(Leu-Pro) and Tetradecanoic Acid Indian J. Microbiol. (IF 1.83) Pub Date : 2021-01-06 Milind Gajbhiye, Balu Kapadnis
The antifungal cyclo-depeptide and the fatty acid were isolated and purified from an indigenous strain of Lactococcus lactis subsp. cremoris. Maximal activity was observed at pH 5.5 and 6.5, and at 30 °C under stationary conditions, which was detected in the culture supernatant 8 h post-inoculation in MRS broth until 22 h. The activity of antifungal compounds in the culture supernatant was sensitive
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Autoinducer-2 Could Affect Biofilm Formation by Food-Derived Bacillus cereus Indian J. Microbiol. (IF 1.83) Pub Date : 2021-01-06 Nari Lee, Myo-Deok Kim, Min-Cheol Lim
Bacillus cereus is a foodborne pathogen and cause a frequent problem due to the biofilms forming in equipment of food production plants. Autoinducer-2 (AI-2) involved in interspecies communication, plays a role in the biofilm formation of B. cereus. In this study, biofilm formation by thirty-nine B. cereus strains isolated from foods produced in Korea was determined. To investigate the effect of AI-2
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Restoring Good Health in Elderly with Diverse Gut Microbiome and Food Intake Restriction to Combat COVID-19 Indian J. Microbiol. (IF 1.83) Pub Date : 2021-01-03 Ligen Yu
COVID-19 continues to be an ongoing global threat. The elderly with underlying health conditions like cardiovascular and lung diseases, diabetes, obesity, are the most vulnerable to this disease. Curing the pre-existing health conditions will greatly increase a person’s resilience to COVID-19 and lower the death rate of the old people. Digestion and immunity form an integrated nutrition acquisition
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Pathogenic Microbial Profile and Antibiotic Resistance Associated with Periodontitis Indian J. Microbiol. (IF 1.83) Pub Date : 2021-01-01 R. Ansiliero, J. M. L. N. Gelinski, Q. L. Samistraro, C. M. Baratto, C. A. Almeida, C. Locatelli
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Recent Clinical Trials on Natural Products and Traditional Chinese Medicine Combating the COVID-19 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-12-30 Shivraj Hariram Nile, Guoyin Kai
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing potentially fatal coronavirus disease-19 (COVID-19), with a significant health and economic burden around the globe. Currently many clinical studies are undergoing but still there is no any specific approved therapy or drug established for effective treatment of COVID-19. This review aimed to analyses various clinical studies
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Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation Indian J. Microbiol. (IF 1.83) Pub Date : 2020-11-19 Sanjay K. S. Patel, Rahul K. Gupta, Sang-Yong Kim, In-Won Kim, Vipin C. Kalia, Jung-Kul Lee
In the present study, Rhus vernicifera laccase (RvLac) was immobilized through covalent methods on the magnetic nanoparticles. Fe2O3 and Fe3O4 nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.3% at optimum incubation and pH of 18 h and 5.8, respectively. The maximum RvLac loading of 156 mg/g
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Sukshmjeevanu in Vedas: The Forgotten Past of Microbiology in Indian Vedic Knowledge Indian J. Microbiol. (IF 1.83) Pub Date : 2020-10-19 Urvashi Kuhad, Gunjan Goel, Pawan K. Maurya, Ramesh C. Kuhad
No one questions the existence of presumptive knowledge of invisible organisms causing disease, decay and destruction mentioned before the discovery of the microbial world by Antonie Van Leeuwenhoek, who was the first to describe the invisible world as per literature available today. However, the knowledge about microbial world by Indian Rishis presented in Sanskrit shlokas or suktas of our traditional
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Potentiation of Antibiotic Activity by a Meldrum’s Acid Arylamino Methylene Derivative against Multidrug-Resistant Bacterial Strains Indian J. Microbiol. (IF 1.83) Pub Date : 2020-10-10 Maria M. C. da Silva, José B. de Araújo-Neto, Ana C. J. de Araújo, Priscilla R. Freitas, Cícera D. de M. Oliveira-Tintino, Iêda M. Begnini, Ricardo A. Rebelo, Luiz E. da Silva, Sandro L. Mireski, Michele C. Nasato, Maria I. L. Krautler, Jaime Ribeiro-Filho, Henrique D. M. Coutinho, Saulo R. Tintino
This study aimed to evaluate the intrinsic antibacterial activity and antibiotic-enhancing effect of an arylamino methylene derivative (MAD) in association with fluoroquinolones. The antibacterial activity against multiresistant Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was analyzed by determining the minimum inhibitory concentration (MIC) using the broth micro dilution method
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Metagenomic Analysis of the Faecal Microbiome of Rats with 1, 2-Dimethylhydrazine Induced Colon Cancer and Prophylactic Whole-Cell Carotenoid Intervention Indian J. Microbiol. (IF 1.83) Pub Date : 2020-10-07 Sankaranarayanan Gomathinayagam, Gothandam Kodiveri Muthukaliannan
Rapidly evolving sequencing technologies have enabled efficient sequencing of complex genomes and metagenomes. Here, we have presented our metagenomic analysis of rat faeces isolated DNA, sequenced using long-read sequencing technology. The microbiome changes in the rat faeces after sixteen weeks of prolonged administration of subcutaneous 1,2 dimethylhydrazine to induce colon carcinogenesis and oral
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Diet, Gut Microbiota and COVID-19 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-28 Praveen Rishi, Khemraj Thakur, Shania Vij, Lavanya Rishi, Aagamjit Singh, Indu Pal Kaur, Sanjay K. S. Patel, Jung-Kul Lee, Vipin C. Kalia
Worldwide, millions of individuals have been affected by the prevailing SARS-CoV-2. Therefore, a robust immune system remains indispensable, as an immunocompromised host status has proven to be fatal. In the absence of any specific antiviral drug/vaccine, COVID-19 related drug repurposing along with various other non-pharmacological measures coupled with lockdown have been employed to combat this infection
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Prevalence of Histidine Decarboxylase Genes of Gram-Positive Bacteria in Surströmming as Revealed by qPCR Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-22 Luca Belleggia, Vesna Milanović, Federica Cardinali, Cristiana Garofalo, Francesca Clementi, Lucia Aquilanti, Andrea Osimani
Histamine is a degradation product of the bacterial decarboxylation of the amino acid histidine; such activity is determined by histidine decarboxylase encoded by a gene cluster, carried by some Gram-positive bacteria, that includes the hdcA gene. In this study, the presence of the hdcA gene in ready-to-eat surströmming samples collected from three producers based in Sweden was directly assessed via
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Efficient Editing of an Adenoviral Vector Genome with CRISPR/Cas9 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-02 Qiang Li, Hui Wang, Chen-yu Gong, Zhao Chen, Jia-xing Yang, Hong-wei Shao, Wen-feng Zhang
Immunotherapy based on genetic modification of T cells has played an important role in the treatment of tumors and viral infections. Moreover, adenoviral vectors engineered with improved safety due to their inability to integrate into the host genome have been key in the clinical application of T cell therapy. However, the commonly used adenoviral vector Ad5 exhibits low efficiency of infection of
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Antifungal Activity In Vitro and In Vivo of Mesquite Extract ( Prosopis glandulosa ) Against Phytopathogenic Fungi Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-02 Arely Nohemí López-Anchondo, Daniel López-de la Cruz, Edgar Gutiérrez-Reyes, José Cristobal Castañeda-Ramírez, Norma Margarita De la Fuente-Salcido
Fungi are the primary infectious agents in plant crops and many post-harvest fungal diseases of fruit and vegetables causing significant economic losses worldwide. Here, the antifungal effect of Prosopis glandulosa extract (PgE) against phytopathogenic fungi was evaluated. The effect with PgE (5, 4, 2, 1, 0%) as AI (%) and radial growth rate reduction (Kr %) were determined in vitro in Colletotrichum
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Integration and Potential Application Ability of Culturable Functional Microorganism in Oil Tea Camellia Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-01 Caixia Liu, Longsheng Chen, Zhilong He, Zhen Zhang, Yanming Xu, Zhigang Li, Yinghe Peng, Nan Deng, Yongzhong Chen
Oil tea Camellia is a major woody oil plant, which has a positive influence on alleviating the contradiction between supply and demand of edible oil in China. Microbial fertilizer for Oil tea Camellia is urgently needed in current production, and it is of great significance to improve the yield and quality. Culturable functional microorganisms are the basis of research and development for microbial
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Factors Affecting Bacterial Adhesion on Selected Textile Fibres Indian J. Microbiol. (IF 1.83) Pub Date : 2020-09-01 Swati Varshney, Abhineet Sain, Deepti Gupta, Shilpi Sharma
In sectors like healthcare and hospitality, it has been realized that fabrics play a pivotal role in transfer of nosocomial infections. However, there is a major gap in drawing correlation between different fibre types and their interaction with microorganisms. Such information is important to formulate guidelines for textile materials for use in these sectors. In the current study, the adherence of
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The Use of the Rare TTA Codon in Streptomyces Genes: Significance of the Codon Context? Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-31 Serhii Silov, Nestor Zaburannyi, Maria Anisimova, Bohdan Ostash
Streptomycetes, Gram-positive bacteria with huge and GC-rich genomes provide an ample example of codon usage bias taken to the extreme. Particularly, in all sequenced to date streptomycete genomes leucyl codon TTA is the rarest one. It is present (usually once or twice) in 70–200 out of 7000–8000 coding sequences that make up a typical streptomycete genome. tRNALeuUAA of streptomycetes, encoded by
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A Preliminary Assessment of Skin Microbiome Diversity of Zebrafish ( Danio rerio ): South African Pet Shop Fish Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-22 Willem G. Coetzer, L. M. Coetzee, E. D. Cason, J. P. Grobler, S. R. Schneider, C. E. Boucher
The zebrafish (Danio rerio) is a well-known model organism used in an array of scientific research fields. Many microbiome studies conducted on fishes have focused on gut microbiome diversity. To our knowledge, no investigations into the skin microbiome diversity of pet shop zebrafish have been performed. In this pilot study we aimed to assess the microbiome diversity composition of different groups
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Do Maternal Microbes Shape Newborn Oral Microbes? Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-22 Shengrong Wu, Fei Yu, Liya Ma, Youhong Zhao, Xin Zheng, Xiaodong Li, Zhiqiang Li, Xiangyi He, Jianye Zhou
Strong evidence suggests that the early composition of the oral microbiota of neonates plays an important role for the postnatal development of the oral health or immune system. However, the relationship between the maternal microbiome and the initial neonatal microbiome remains unclear. In this study, 25 pregnant women and their neonates were recruited, and the samples were collected from the maternal
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Protective Functions of Group 3 Late Embryogenesis Abundant (G3LEA) Proteins in Enterococcus faecium During Vancomycin Treatment Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-16 Ahran Song, Boyong Kim, Eunyoung Kim, Jae Hwi Sung, Yoonjin Park, Sohyeon Park, Taegun Park, Jin Kwan Kim, Yoonhwa Jeong, Seung Gwan Lee
Late embryogenesis abundant (LEA) proteins protect organisms from various environmental stresses; however, the underlying mechanism of LEA mediated therapeutic evasion is still unclear in both eukaryotes and prokaryotes. In this study, group 3 LEA protein (G3LEA) of vancomycin-resistant Enterococcus faecium under sublethal concentration of vancomycin stress was evaluated and shown to have two functions:
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Human Gut Microbiota and Mental Health: Advancements and Challenges in Microbe-Based Therapeutic Interventions Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-07 Helianthous Verma, Sonika Phian, Priya Lakra, Jasleen Kaur, Sanjukta Subudhi, Rup Lal, Charu Dogra Rawat
Gut microbes play prime role in human health and have shown to exert their influence on various physiological responses including neurological functions. Growing evidences in recent years have indicated a key role of gut microbiota in contributing to mental health. The connection between gut and brain is modulated by microbes via neural, neuroendocrinal and metabolic pathways that are mediated through
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Nutritional Enhancement of Chicken Feather Waste by Bacillus aerius NSMk2 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-03 Ranjeeta Bhari, Manpreet Kaur, Ram Sarup Singh
Keratinous waste is the bulk by-product of various livestock industries. Slow natural degradation of keratin and less efficient chemical hydrolysis imposes challenge for the search of alternative recycling methods. Keratin degrading microbes hydrolyse keratin to soluble peptides and amino acids. Bacillus aerius NSMk2 showed great potential for hydrolysis of chicken feather waste. Bacillus aerius NSMk2
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A Recent Overview of Microbes and Microbiome Preservation. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-07-01 Om Prakash,Yogesh Nimonkar,Dhananjay Desai
Microbes are mediators in almost all ecosystem processes and act as a pivotal game changer in various ecological activities, globally. Therefore, understanding of microbial community structure and related functions in different environmental and micro-environmental niches is not only critical, but also a matter of greatest importance. Due to our inability to cultivate and preserve all sorts of microorganisms
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In Vitro Cytotoxicity Study of Cyclophosphamide, Etoposide and Paclitaxel on Monocyte Macrophage Cell Line Raw 264.7 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-18 Ankush Yadav, Mrinal Kanti Mandal, Kashyap Kumar Dubey
The presence of antineoplastic compounds in aquatic ecosystem is an emerging challenge for the society. Antineoplastic compounds released into the aquatic environment exhibit a potential threat to normal aquatic life. Particularly, antineoplastic compounds are responsible for direct or indirect interference with the cellular DNA of an organism and cause toxicity to cells. The present study focused
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The Sensitivity Modifying Activity of Nerolidol and α-Bisabolol Against Trichophyton spp Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-13 Josenildo Cândido de Oliveira, Ânderson de Vasconcelos Pinto, César Augusto Costa de Medeiros, Hellen Aparecida Silva Ponte, Fillipe de Oliveira Pereira
Trichophyton spp. is one of the main causative agents of dermatophytosis such as tinea ungium and tinea pedis. Resistance to antifungal drugs is a significant clinical problem in dermatophytosis. The main molecular mechanism of antifungal resistance to conventional therapy in dermatophytes is the expression of efflux pumps. Efforts aimed at improving the efficacy of current antifungals such as griseofulvin
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Evaluation of the Fermentative Capacity of Saccharomyces cerevisiae CAT-1 and BB9 Strains and Pichia kudriavzevii BB2 at Simulated Industrial Conditions Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-10 Adriana Menezes Olivo Fernandes, Nayara Fernanda Lisbôa Garcia, Gustavo Graciano Fonseca, Rodrigo Simões Ribeiro Leite, Marcelo Fossa da Paz
The search for promising yeasts that surpass the fermentative capacity of commercial strains, such as Saccharomyces cerevisiae CAT-1, is of great importance for industrial ethanol processes in the world. Two yeasts, Pichia kudriavzevii BB2 and Saccharomyces cerevisiae BB9, were evaluated in comparison to the industrial yeast S. cerevisiae CAT-1. The objective was to evaluate the performance profile
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Deploying Biomolecules as Anti-COVID-19 Agents. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-09 Sanjay K S Patel,Jung-Kul Lee,Vipin C Kalia
Severe acute respiratory syndrome coronavirus (SARS-CoV-2) known as COVID-19 has emerged as a major threat to human existence. COVID-19 seems to have undergone adaptive evolution through an intermediate host, most likely bats. The flu leads to severe pneumonia that causes respiratory and multi-organ failure. The absence of any known treatment procedures, drugs, or vaccines has created panic around
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Insights into Metatranscriptome, and CAZymes of Buffalo Rumen Supplemented with Blend of Essential Oils Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-06 Anju Kala, D. N. Kamra, N. Agarwal, L. C. Chaudhary, C. G. Joshi
The aim of this study was to investigate the rumen microbial diversity and functionality in buffaloes fed with a blend of essential oils (BEO) using LSD switch over design. The BEO consisting of blend of Trachyspermum copticum (Ajwain) oil, Cymbopogon citratus (lemon grass) oil and Syzygium aromaticum (clove bud) oleoresin mixed in equal proportion, was fed at the rate of 0, 0.75 and 1.5 ml/100 kg
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Purification and Enzymatic Properties of a Difunctional Glycoside Hydrolase from Aspergillus oryzae HML366 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-06-06 Yongling Qin, Yue Fu, Qiqian Li, Fengfeng Luo, Haiyan He
In the study, an extracellular enzyme HML CBH1 was purified from the fermentation solution of Aspergillus oryzae HML366, and characterized by biological and molecular analysis. Following the culturing of A. oryzae HML366 under the optimized conditions for enzyme production, an enzyme named HML CBH1 with a molecular weight of 48 kDa was purified using 3000 Da cellulose ultrafiltration column and anion
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Toxicity Analysis of Recombinant L-asparaginase I and II in Zebrafish Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-30 Susan Aishwarya Suresh, Selvarajan Ethiraj, K. N. Rajnish
L-asparaginases are extensively applied in the treatment of Acute Lymphoblastic Leukemia (ALL). The treatment regime of ALL consists of asparaginase from E. coli or Erwinia. The survival rate post-chemotherapy has increased to < 90% in recent years. Asparaginase therapy has also resulted in numerous toxicities to patients receiving therapy. This study demonstrates the reaction of normal cells of Danio
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Biogenic Silver Nanoparticles: Evaluation of Their Biological and Catalytic Potential Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-27 Bhawna Sharma, Indu Singh, Somvir Bajar, Seema Gupta, Hemant Gautam, Pradeep Kumar
The biogenic tailoring of silver nanoparticles using plant extract is becoming an attractive approach in the current scenario. Manilkara zapota (MZ) is well known for its antibacterial, hepato-protective, anti-inflammatory, anti-tussive, anti-fungal, anti-tumour, and free radical scavenging potential. Its plants extract is a rich source of secondary metabolites. Nowadays, silver nanoparticles (AgNPs)
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Persistence of the antifungal capacity of a fraction of Jacquinia macrocarpa plant against Fusarium verticillioides after continuous exposure Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-27 Iliana Jacqueline Muñoz-Ochoa, Maribel Plascencia-Jatomea, Francisco Javier Cinco-Moroyoqui, Daniel Fernando Valenzuela-Cota, Mario Onofre Cortez-Rocha, María Alba Guadalupe Corella-Madueño, Ema Carina Rosas-Burgos
This study aimed to determine the ability of Fusarium verticillioides in developing mechanisms to counteract the antifungal effect of a fraction from Jacquinia macrocarpa plant extract (JmAF), as well as the morphological and physiological changes that occur during its exposure. The fungus was exposed to JmAF during consecutive periods. A culture sample was taken weekly to determine radial growth,
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Human Gut Microbiome Response to Short-Term Bifidobacterium -Based Probiotic Treatment Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-25 Natalia Naumova, Tatiana Alikina, Alexey Tupikin, Anna Kalmykova, Galina Soldatova, Valentin Vlassov, Marsel Kabilov
Gut microbiota is believed to play a crucial role in modulating obesity in humans, and probiotics affecting gut microbiota can alleviate some of the obesity-related health complications. The study was aimed to investigate changes in the composition of the gut microbiome in obese humans due to short-term (2 weeks) treatment of obese patients with a probiotic preparation containing Bifidobacterium longum
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Comparison Between the Gut Microbiota in Different Gastrointestinal Segments of Large-Tailed Han and Small-Tailed Han Sheep Breeds with High-Throughput Sequencing Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-25 Guangli Yang, Shuhong Zhang, Zhiqiang Li, Jie Huang, Yan Liu, Ying Liu, Qiankun Wang, Xiangyu Li, Yongfeng Yan, Ming Li
Commensal microorganisms are essential to the normal development and function of many aspects of animal biology. However, the dynamic shift patterns of the microbiota of different gut segments in sheep and the correlation between fat type large-tailed phenotype and microbiota remain poorly unknown. This study therefore sought to assess the composition and distribution of the intestinal microbiome,
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Optimization of Cr 6+ Removal by Bacillus subtilis Strain SZMC 6179J from Chromium-Containing Soil Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-23 Jianghong Liu, Jian Xue, Xiaohang Wei, Huimin Su, Ruidan Xu
The removal rate of Cr6+ has been explored by the optimized removal conditions. Five Cr-resistant strains were isolated from chromium-contained soil. The most efficient strain S1 was identified as Bacillus subtilis strain SZMC 6179J through 16S rDNA. Response surface methodology (RSM) was used to investigate the effects of four independent variables, including initial pH, initial Cr6+ concentration
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Biomethanol Production from Methane by Immobilized Co-cultures of Methanotrophs. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-22 Sanjay K S Patel,Rahul K Gupta,Virendra Kumar,Sanath Kondaveeti,Anurag Kumar,Devashish Das,Vipin Chandra Kalia,Jung-Kul Lee
Methanol production by co-culture of methanotrophs Methylocystis bryophila and Methyloferula stellata was examined from methane, a greenhouse gas. Co-culture exhibited higher methanol yield of 4.72 mM at optimum ratio of M. bryophila and M. stellata (3:2) compared to individual cultures. The immobilized co-culture within polyvinyl alcohol (PVA) showed relative efficiency of 90.1% for methanol production
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A Lesson Learned from the Outbreak of COVID-19 in Korea. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-14 Kyoo-Man Ha
Despite the fact that making all efforts to deal with COVID-19 outbreak, Korea is in urgent need of moving away from a divided community approach and adopting a total community approach. Further, the nation needs to fully establish and activate the national emergency operation framework.
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Bacterial and Archaeal Communities within an Ultraoligotrophic, High-altitude Lake in the Pre-Himalayas of the Qinghai-Tibet Plateau. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-13 Rong Wang,Rui Han,Qifu Long,Xiang Gao,Jiangwa Xing,Guoping Shen,Derui Zhu
Puma Yumco Lake (PYL) is an ultraoligotrophic freshwater lake that sits an altitude of 5030 m within the Qinghai-Tibet Plateau of China. The bacterial and archaeal diversity of the lake remains poorly understood, despite their potential to inform on biogeochemical cycling and environment-microbial associations in these unique environments. Here, the bacterial and archaeal communities of PYL were investigated
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Tryptic Mapping Based Structural Insights of Endo-1, 4-β-Xylanase from Thermomyces lanuginosus VAPS-24. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-11 Brian N Mathibe,Samkelo Malgas,Layla Radosavljevic,Vishal Kumar,Pratyoosh Shukla,Brett I Pletschke
An endo-1,4-β-xylanase, XynA, from Thermomyces lanuginosus VAPS-24, was purified to homogeneity and exhibited a molecular mass of approximately 20 kDa. The protein sequence of XynA was found to be similar to those of other Thermomyces lanuginosus derived xylanases and, as a result, could be used as a model enzyme for understanding the protein structure–activity relationship and facilitating protein
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Atrazine Bioremediation and Its Influence on Soil Microbial Diversity by Metagenomics Analysis. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-07 Pooja Bhardwaj,Kunvar Ravendra Singh,Niti B Jadeja,Prashant S Phale,Atya Kapley
Pesticide accumulation in agricultural soils is an environmental concern, often addressed through distinct bioremediation strategies. This study has tried to analyze various soil bioremediation options viz., biostimulation, bioaugmentation, and natural attenuation in terms of efficiency and the response of autochthonous microbial flora by using atrazine as a model contaminant. Soil mesocosms were established
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Relationship Between Main Channel Structure of Catalases and the Evolutionary Direction in Cold-Adapted Hydrogen Peroxide-Tolerant Exiguobacteium and Psychrobacter. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-05-06 Yoshiko Hanaoka,Hideyuki Kimoto,Kazuaki Yoshimume,Isao Hara,Hidetoshi Matsuyama,Isao Yumoto
Catalase has crucial role in adaptive response to H2O2. Main channel structure responsible for substrate selectivity was estimated to understand the relationship between the evolutionary direction of catalases from Exiguobacterium oxidotolerans and Psychrobacter piscatorii which survive in cold and high concentration of hydrogen peroxide, and their catalytic property. E. oxidotolerans catalase (EKTA)
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Complement Evasion Strategies of Human Pathogenic Bacteria. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-24 Shikhar Sharma,Rakesh Bhatnagar,Deepak Gaur
Human pathogens need to overcome an elaborate network of host defense mechanisms in order to establish their infection, colonization, proliferation and eventual dissemination. The interaction of pathogens with different effector molecules of the immune system results in their neutralization and elimination from the host. The complement system is one such integral component of innate immunity that is
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Overview of Alternaria alternata Membrane Proteins. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-22 Ruyi Wang,Peng Zhao,Xizhen Ge,Pingfang Tian
Alternaria species are mainly saprophytic fungi, but some pathotypes of Alternaria alternata infect economically important plants including cereal crops, vegetables and fruits. Specially, A. alternata generates toxins which contaminate food and feed. To date, management of A. alternata relies primarily on fungicides. However, the control efficacy in most cases is below expectation due to ubiquity of
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Combinatorial Biobleaching of Mixedwood Pulp with Lignolytic and Hemicellulolytic Enzymes for Paper Making. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-20 Steffy Angural,Monika Rana,Alisha Sharma,Rahul Warmoota,Neena Puri,Naveen Gupta
Microbial enzymes are the safe alternatives to chemical based bleaching of pulp in paper mills. For effective biobleaching, both hemicellulolytic and lignolytic enzymes are required. This study reports laccase (L) + xylanase (X) and laccase (L) + mannanase (M) enzyme concoctions for pulp biobleaching derived from Bacillus sp. LX and Bacillus sp. LM isolated from the decaying organic matter. All enzymes
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Understanding SARS-CoV-2: Genetic Diversity, Transmission and Cure in Human. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-20 Abhay Bajaj,Hemant J Purohit
As the SARS-CoV-2 virus race around the world across the different population, there needs to be a consolidated effort to understand the divergence of demographically distributed strains. The emerging trends in SARS-CoV-2 genome data show specific mutation and genetic diversity, which could provide the basis to develop a cocktail of vaccine and may also be used to develop the region-specific diagnostic
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Dried Biomass of Arthrospira platensis Inhibits Growth of Aureobasidium pullulans LW14 and Some Bacteria When Added to Unpasteurised Apple Juice. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-18 Łukasz Wajda,Zuzanna Rękas,Tomasz Tarko,Aleksandra Duda-Chodak,Aleksandra Liebersbach,Małgorzata Makarewicz
The main goal was to evaluate if dried cyanobacterium (Arthrospira platensis) biomass added to unpasteurised apple juice could inhibit growth of fungi dominant in apples and which fraction of cyanobacterium biomass contributed to this phenomenon. A. platensis biomass, its protein and/or polysaccharide fraction were added to the sterilised apple juice inoculated with Aureobasidium pullulans LW14 and
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Prediction of Transcription Factors and Their Involvement in Regulating Rifamycin Production in Amycolatopsis mediterranei S699. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-18 Nirjara Singhvi,Vipin Gupta,Priya Singh,Om Prakash,Andreas Bechthold,Yogendra Singh,Rup Lal
Amycolatopsis mediterranei S699 produces rifamycin B and successors of this strain are in use for the industrial production of rifamycin B. Semisynthetic derivatives of rifamycin B are used against Mycobacterium tuberculosis that causes tuberculosis. Although the rifamycin biosynthetic gene cluster was characterized two decades ago, the regulation of rifamycin B biosynthesis in Amycolatopsis mediterranei
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Bioactive Pigments from Isolated Bacteria and Its Antibacterial, Antioxidant and Sun Protective Application Useful for Cosmetic Products. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-15 Janki Choksi,Jaykant Vora,Neeta Shrivastava
Bacterial pigments are the unique and sustainable source of bioactive colour compounds used in cosmetics, food, textiles, printing and pharmaceutical products. Here, we report the pigment-producing isolates and their biological activities that could be benefited for different industries including cosmeceuticals. In this study, a total of 19 pigment-producing bacteria were isolated and purified from
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VBA-AMF: A VBA Program Based on the Magnified Intersections Method for Quantitative Recording of Root Colonization by Arbuscular Mycorrhizal Fungi. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-10 Wentao Hu,Lan Pan,Hui Chen,Ming Tang
The extent of mycorrhizal fungi colonization is an important factor for determining the function of mycorrhizal fungi in fungi–host interaction, and quantifying the extent of mycorrhizal fungi colonization is a fundamental and essential task for researchers engaged in mycorrhizal studies. Intersect methods, such as the gridline intersect and magnified intersections methods, are accurate and objective
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Comparative Genomics of a Paddy Field Bacterial Isolate Ochrobactrum sp. CPD-03: Analysis of Chlorpyrifos Degradation Potential. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-02 Tanmaya Nayak,Ananta N Panda,Khushbu Kumari,Tapan Kumar Adhya,Vishakha Raina
Ochrobactrum genus is known to catabolize aromatic compounds. This study reports a complete genome sequence of Ochrobactrum sp. CPD-03 (~ 4.6 Mb of chromosomal features) responsible for chlorpyrifos (CP) isolated form a paddy field (20.3588° N, 85.8333° E) in Bhubaneswar, India. A comparative genomics approach was performed between CPD-03 and eight closely related genomes of other Ochrobactrum strains
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Optimization of Biosynthesis Conditions for the Production of Exopolysaccharides by Lactobacillus plantarum SP8 and the Exopolysaccharides Antioxidant Activity Test. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-04-02 Le Zhang,Bo Zhao,Chen-Jian Liu,En Yang
Lactobacillus plantarum SP8, isolated from traditional Chinese pickle juice, was utilized for the production of exopolysaccharides (EPSs), but the EPS yield was low under normal MRS medium. The single factor experiment and response surface methodology were used to optimize the medium components and culture conditions and the optimal conditions for EPS production were successfully obtained. Results
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16S rRNA Gene-Based Analysis Reveals the Effects of Gestational Diabetes on the Gut Microbiota of Mice During Pregnancy. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-03-16 Ziting Yao,Yu Long,Juan Ye,Pu Li,Yonghua Jiang,Yanming Chen
Hyperglycemia is one of the metabolic characteristics of gestational diabetes mellitus (GDM). Considering that GDM is able to cause changes in the gut bacterial community and function in the mother’s intestine compared with healthy pregnant women, we aimed to clarify the correlation between hyperglycemia and gut microbiota in a GDM mouse model. Mice were divided into four groups: CE0, GDME0, CE18,
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Commentary: Reconciling Hygiene and Cleanliness: A New Perspective from Human Microbiome Indian J. Microbiol. (IF 1.83) Pub Date : 2020-03-12 Boxuan Yu; Bowei Yu; Ligen Yu
Human beings have co-evolved with the microorganisms in our environment for millions of years, and have developed into a symbiosis in a mutually beneficial/defensive way. Human beings have significant multifaceted relationships with the diverse microbial community. Apart from the important protective role of microbial community exposure in development of early immunity, millions of inimitable bacterial
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Difference of Phenotype and Genotype Between Human and Environmental: Isolated Vibrio cholerae in Surabaya, Indonesia Indian J. Microbiol. (IF 1.83) Pub Date : 2020-03-11 Kayo Osawa; Katsumi Shigemura; Koichi Kitagawa; K. Kuntaman; Ni Made Mertaniasih; Wahyu Setyarini; Dita Arizandy; Dadik Rahadjo; Ro Osawa; Toshiro Shirakawa; Masato Fujisawa
Cholera due to Vibrio cholerae has been spreading worldwide, although the reports focusing on Indonesian V. cholerae are few. In this study, in order to investigate how V. cholerae transmitted to human from environment. We extended an epidemiological report that had investigated the genotype of V. cholerae isolated from human pediatric samples and environmental samples. We examined 44 strains of V
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Zoonosis: An Emerging Link to Antibiotic Resistance Under “One Health Approach” Indian J. Microbiol. (IF 1.83) Pub Date : 2020-03-04 Nishant A. Dafale; Shweta Srivastava; Hemant J. Purohit
Current scenario in communicable diseases has generated new era that identifies the “One health” approach to understand the sharing and management of etiological agents with its impact on ecosystem. Under this context the relevance of zoonotic diseases generates major concern. The indiscriminate and higher use of antibiotics in animal husbandry creates substantial pressure on the gut microbiome for
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Cytotoxic Effect of Prodigiosin, Natural Red Pigment, Isolated from Serratia marcescens UFPEDA 398 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-02-20 J. C. L. Lapenda; V. P. Alves; M. L. Adam; M. D. Rodrigues; S. C. Nascimento
Prodigiosin is a secondary metabolite, with red pigmentation, produced by Serratia marcescens. Red pigment is a natural alkaloid whose chemical structure has three pyrrole rings. Prodigiosin has been described for several biological activities, including antitumor, inducing apotosis in T and B lymphocytes. This work aimed to evaluate the cytotoxic activity of prodigiosin in NCHI-292, HEp-2, MCF-7 and
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A Review on Occurrence of Pesticides in Environment and Current Technologies for Their Remediation and Management Indian J. Microbiol. (IF 1.83) Pub Date : 2020-02-14 K. S. Rajmohan; Ramya Chandrasekaran; Sunita Varjani
Pesticides are the chemicals used to prevent plant diseases, weeds, pests and to enhance the quality of the food products. The uniqueness of their chemical structure, and/or their interactions with the environment characterize the nature of pesticides. In most scenarios, the end users such as farmers and consumers, who know the serious effects of pesticides cannot translate this awareness into their
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Functional Analysis of a Glutamine Biosynthesis Protein from a Psychrotrophic Bacterium, Cryobacterium soli GCJ02 Indian J. Microbiol. (IF 1.83) Pub Date : 2020-02-06 Chunjie Gong; Xihuo You; Shuyang Zhang; Dongsheng Xue
A putative glutamine synthetase (GS) was detected in a psychrophilic bacterium, Cryobacterium soli GCJ02. For gaining greater insight into its functioning, the gene was cloned and expressed in a heterologous host, Escherichia coli. The monomer enzyme with a molecular weight of 53.03 kDa was expressed primarily in cytosolic compartment. The enzyme activity was detected using glutamate and ATP. The optimum
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Evaluation of DNA Methylation Changes by CRED–RA Analysis Following Prednisone Treatment of Endophyte, Fusarium oxysporum Indian J. Microbiol. (IF 1.83) Pub Date : 2020-01-24 K. Harshitha; R. Aswati Nair
Endophytes that represent a sub-set of plant resident microbes are a reservoir of bioactive metabolites. Many of the secondary metabolite biosynthetic gene clusters of endophytes are silent under axenic culture conditions. Epigenetic reprogramming of such cryptic pathways is possible by use of small molecule modulators like prednisone. Methylation changes induced by prednisone, a hypomethylating epigenetic
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Microbiome: A New Lease to Microbiology. Indian J. Microbiol. (IF 1.83) Pub Date : 2020-01-11 Vipin Gupta,Utkarsh Sood,Roshan Kumar,Rup Lal,Vipin Chandra Kalia