样式: 排序: IF: - GO 导出 标记为已读
-
Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-03-12 Caixia Pei, Xinlv Peng, Yiran Wu, Runmiao Jiao, Tiehai Li, Siming Jiao, Lei Zhou, Jianjun Li, Yuguang Du, Eika W. Qian
Eukaryotic sialyltransferases play key roles in many physiological and pathological events. The expression of active human recombinant sialyltransferases in bacteria is still challenging. In the current study, the genes encoding human -acetylgalactosaminide α2,6-sialyltransferase V (hST6GalNAc V) and -acetylgalactosaminide α2,6-sialyltransferase VI (hST6GalNAc VI) lacking the -terminal transmembrane
-
Metabolism of phenolic compounds catalyzed by Tomato CYP736A61 Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-03-07 Thanh Dat Mai, Hyun Min Kim, Seo Young Park, Sang Hoon Ma, Ju Hui Do, Won Choi, Hye Min Jang, Hyeon Bae Hwang, Eun Gyeong Song, Jae Sung Shim, Young Hee Joung
Cytochrome P450s (CYPs) regulate plant growth and stress responses by producing diverse primary and secondary metabolites. However, the function of many plant CYPs remains unknown because, despite their structural similarity, predicting the enzymatic activity of CYPs is difficult. In this study, one member of the CYP736A subfamily (CYP736A61) from tomatoes was isolated and characterized its enzymatic
-
Application of Thermomyces lanuginosus polygalacturonase produced in Komagataella phaffii in biomass hydrolysis and textile bioscouring Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-03-02 Luana Assis Serra, Thais Demarchi Mendes, Janice Lisboa De Marco, João Ricardo Moreira de Almeida
In this work, the polygalacturonase (TL-PG1) from the thermophilic fungus was heterologously produced for the first time in the yeast . The TL-PG1 was successfully expressed under the control of the AOX1 promoter and sequentially purified by His-tag affinity. The purified recombinant pectinase exhibited an activity of 462.6 U/mL toward polygalacturonic acid under optimal conditions (pH 6 and 55 ˚C)
-
Engineered Zea mays phenylalanine ammonia-lyase for improve the catalytic efficiency of biosynthesis trans-cinnamic acid and p-coumaric acid Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-23 Jiangmei Zheng, Ruobin Sun, Dan Wu, Pengcheng Chen, Pu Zheng
Phenylalanine ammonia-lyase (PAL) plays a pivotal role in the biosynthesis of phenylalanine. PAL from (ZmPAL2) exhibits a bi-function of direct deamination of L-phenylalanine (L-Phe) or L-tyrosine(-L-Tyr) to form -cinnamic acid or -coumaric acid. -Cinnamic acid and -coumaric acid are mainly used in flavors and fragrances, food additives, pharmaceutical and other fields. Here, the Activity of ZmPAL2
-
-
Surface expression of carbonic anhydrase on E. coli as a sustainable approach for enzymatic CO2 capture Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-17 Juned Ali, Shazia Faridi, Amuliya Kashyap, Rubia Noori, Meryam Sardar
The utilisation of carbonic anhydrase (CA) in CO sequestration is becoming prominent as an efficient, environment friendly and rapid catalyst for capturing CO from industrial emissions. However, the application of CA enzyme in soluble form is constrained due to its poor stability in operational conditions of CO capture and also production cost of the enzyme. Addressing these limitations, the present
-
Economical one-pot synthesis of isoquercetin and D-allulose from quercetin and sucrose using whole-cell biocatalyst Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-15 Qi-Yang Wang, Hao-Yu Wang, Wei-Guo Zhang, Jian-Zhong Xu
-
Biomanufacture of L-homoserine lactone building block: A strategy for preparing γ-substituted L-amino acids by modular reaction Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-07 Yuguang Wang, Mengjing Wu, Huifang Zheng, Dongmei Wu, Panpan Yao, Wenjing Li, Kexin Jin, Xinjun Yu
A strain high-performance of esterase producing bacteria was screened from soil, which could selectively hydrolyze -homoserine lactone from its racemate to achieve the resolution of - homoserine lactone with more than 99% in 48% yield. -homoserine lactone building block was then converted to -α-amino-γ-bromobutyronic acid chiral blocks, which reacted with various nucleophilic reagent modules could
-
Efficient biosynthesis of prunin in methanol cosolvent system by an organic solvent-tolerant α-L-rhamnosidase from Spirochaeta thermophila Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-06 Chen-Mu Luo, Li-Fan Ke, Xiang-Yu Huang, Xiao-Yan Zhuang, Ze-Wang Guo, Qiong Xiao, Jun Chen, Fu-Quan Chen, Qiu-Ming Yang, Yi Ru, Hui-Fen Weng, An-Feng Xiao, Yong-Hui Zhang
Prunin of desirable bioactivity and bioavailability can be transformed from plant-derived naringin by the key enzyme α-L-rhamnosidase. However, the production was limited by unsatisfactory properties of α-L-rhamnosidase such as thermostability and organic solvent tolerance. In this study, biochemical characteristics, and hydrolysis capacity of a novel α-L-rhamnosidase from (St-Rha) were investigated
-
Optimizing the enzymatic production of biolubricants by the Taguchi method: Esterification of the free fatty acids from castor oil with 2-ethyl-1-hexanol catalyzed by Eversa Transform 2.0 Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-05 Rodolpho R.C. Monteiro, Maria M.F. de Melo Neta, Wesley S. Rocha, Jorge B. Soares, F. Murilo T. de Luna, Roberto Fernandez-Lafuente, Rodrigo S. Vieira
The solvent-free esterification of the free fatty acids (FFAs) obtained by the hydrolysis of castor oil (a non-edible vegetable oil) with 2-ethyl-1-hexanol (a branched fatty alcohol) was catalyzed by different free lipases. Eversa Transform 2.0 (ETL) features surpassed most commercial lipases. Some process parameters were optimized by the Taguchi method (L16’). As a result, a conversion over 95% of
-
Preliminary identification and semi-quantitative characterization of a multi-faceted high-stability alginate lyase from marine microbe Seonamhaeicola algicola with anti-biofilm effect on Pseudomonas aeruginosa Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-02-01 Shuaiting Yun, Jinping Huang, Mingjing Zhang, Xueting Wang, Xiaochen Wang, Yanxia Zhou
-
Application of extremophile cell factories in industrial biotechnology Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-30 Yuzhou Wang, Jinyi Qian, Tianqiong Shi, Yuetong Wang, Qiang Ding, Chao Ye
Due to the extreme living conditions, extremophiles have unique characteristics in morphology, structure, physiology, biochemistry, molecular evolution mechanism and so on. Extremophiles have superior growth and synthesis capabilities under harsh conditions compared to conventional microorganisms, allowing for unsterilized fermentation processes and thus better performance in low-cost production. In
-
ω-Transaminase-catalyzed synthesis of (R)-2-(1-aminoethyl)-4-fluorophenol, a chiral intermediate of novel anti-tumor drugs Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-26 Quan Luo, Guan Zhou, Zhongxia Li, Jiangpeng Dong, Hang Zhao, Huifang Xu, Xuefeng Lu
The chiral amine (R)-2-(1-aminoethyl)-4-fluorophenol has attracted increasing attentions in recent years in the field of pharmaceuticals because of its important use as a building block in the synthesis of novel anti-tumor drugs targeting tropomyosin receptor kinases. In the present study, a ω-transaminase (ωTA) library consisting of 21 (R)-enantioselective enzymes was constructed and screened for
-
Tailoring a cellulolytic enzyme cocktail for efficient hydrolysis of mildly pretreated lignocellulosic biomass Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-24 Eva Balaguer Moya, Berta Syhler, Giuliano Dragone, Solange I. Mussatto
-
A novel pectate lyase with high specific activity from Bacillus sp. B58-2: Gene cloning, heterologous expression and use in ramie degumming Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-16 Sijia Liu, Yan Qin, Qingyan Wang, Jing Zhang, Jin Zhou, Baoxiang He, Xinquan Liang, Liang Xian, Junhua Wu
Pectinase plays a crucial role in ramie degumming. A gene encoding a putative pectate lyase from Bacillus sp. strain B58–2 was cloned and heterologously expressed in Escherichia coli. The amplified gene BvelPL1 encoded a mature protein of 400 amino acids. BvelPL1 shared the highest amino acid sequence identity (78.75%) with the enzymatically characterized pectate lyase Pel from Bacillus subtilis strain
-
Enhanced theanine production with reduced ATP supply by alginate entrapped Escherichia coli co-expressing γ-glutamylmethylamide synthetase and polyphosphate kinase Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-15 Do Hyun Cho, Suwon Kim, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Hee Taek Kim, See-Hyoung Park, Kyungmoon Park, Shashi Kant Bhatia, Yung-Hun Yang
L-theanine is an amino acid with a unique flavor and many therapeutic effects. Its enzymatic synthesis has been actively studied and γ-Glutamylmethylamide synthetase (GMAS) is one of the promising enzymes in the biological synthesis of theanine. However, the theanine biosynthetic pathway with GMAS is highly ATP-dependent and the supply of external ATP was needed to achieve high concentration of theanine
-
A concerted enzymatic de-structuring of lignocellulosic materials using a compost-derived microbial consortia favoring the consolidated pretreatment and bio-saccharification Enzyme Microb. Technol. (IF 3.4) Pub Date : 2024-01-02 Gunasekaran Rajeswari, Vinod Kumar, Samuel Jacob
-
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-26 Enshi Liu, Martha Inés Vélez Mercado, Fernando Segato, Mark R. Wilkins
Lignin depolymerization, which enables the breakdown of a complex and heterogeneous aromatic polymer into relatively uniform derivatives, serves as a critical process in valorization of lignin. Enzymatic lignin depolymerization has become a promising biological strategy to overcome the heterogeneity of lignin, due to its mild reaction conditions and high specificity. However, the low solubility of
-
Evaluation of alternate hosts for recombinant expression of a reductive dehalogenase Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-22 Rabeya Rahmatullah, Christopher P. Marquis
Organohalides are recalcitrant, toxic environmental pollutants. Reductive dehalogenase enzymes (RDases) found in organohalide respiring bacteria (OHRB) utilise organohalides as electron acceptors for cellular energy and growth, producing lesser-halogenated compounds. Consequently, microbial reductive dehalogenation via organohalide respiration represents a promising solution for clean-up of organohalide
-
Expression of the two-component regulator StyS/StyR enhanced transcription of the styrene monooxygenase gene styAB and indigo biosynthesis in Escherichia coli Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-16 Sheng Yin, Yujie Li, Jialing Hou
Indigo, an economically important dye, could be biosynthesized from indole by catalysis of the styrene monooxygenase StyAB. To enhance indigo biosynthesis, the styAB gene and its transcription regulator gene styS/styR in styrene catabolism were cloned from Pseudomonas putida and coexpressed in Escherichia coli. The presence of the intact regulator gene styS/styR dramatically increased the transcriptional
-
Cloning and heterologous expression of Fusarium oxysporum nitrilase gene in Escherichia coli and evaluation in cyanide degradation Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-19 Azamsadat Moosavizadeh, Mostafa Motallebi, Zahra Moghaddassi Jahromi, Lukhanyo Mekuto
Cyanide is widely utilized in the extraction of precious metal extraction even though it has been deemed as the most toxic compound. Fusarium oxysporum has been shown to degrade cyanide through the activity of the Nitrilase enzyme. In this study, the coding sequence of nitrilase gene from F. oxysporum genomic DNA was optimized for cloning and expression in E. coli. The pUC57 containing synthetic optimized
-
Split-GFP complementation at the bacterial cell surface for antibody-free labeling and quantification of heterologous protein display Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-20 David Gercke, Florian Lenz, Joachim Jose
-
Efficient production of γ-aminobutyric acid using engineered Escherichia coli whole-cell catalyst Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-13 Fangfang Chang, Yuheng Wang, Jie Zhang, Tao Tu, Huiying Luo, Huoqing Huang, Yingguo Bai, Xing Qin, Yaru Wang, Bin Yao, Yuan Wang, Xiaolu Wang
-
Nanomaterial-based methods for sepsis management Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-15 Iraj Alipourfard, Mohammad Darvishi, Arghavan khalighfard, Farhood Ghazi, Ahmad Mobed
-
Advances in xanthine biosensors and sensors: A review Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-14 Jyoti Ahlawat, Minakshi Sharma, Chandra S. Pundir
Xanthine is derived from hypoxanthine by xanthine oxidase (XOD), a flavoprotein containing molybdenum and non-haem iron, sulfur and from guanine by guanine deaminase enzyme. Xanthine is oxidized into uric acid by XOD. Xanthine is used as an indicator of fish freshness, based on the reactions in which ATP is degraded into xanthine and its quantity increases with time of fish death. Fresh fish meat is
-
Stereoselective synthesis of (R)-(+)−1-(1-naphthyl)ethylamine by ω-amine transaminase immobilized on amino modified multi-walled carbon nanotubes and biocatalyst recycling Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-13 Shuai Qiu, Yu-Tong Cui, Tong-Tong Wang, Fang-Fang Fan, Chang-Jiang Lyu, Jun Huang
Immobilized enzymes exhibit favorable advantages in biocatalysis, such as high operation stability, feasible reusability, and improved organic solvents tolerance. Herein, an immobilized ω-amine transaminase AtATA@MWCNTs-NH2 is successfully prepared using amino modified multi-walled carbon nanotubes as carrier and glutaraldehyde as crosslinker. Under the optimum immobilization conditions, the activity
-
The potential of R. toruloides mevalonate pathway genes in increasing isoprenoid yields in S. cerevisiae: Evaluation of GGPPS and HMG-CoA reductase Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-13 Sri Harsha Adusumilli, Govinda Rao Dabburu, Manish kumar, Prateek Arora, Banani Chattopadhyaya, Diptimayee Behera, Anand Kumar Bachhawat
-
Family 3 CBM improves the biochemical properties, substrate hydrolysis and coconut oil extraction by hemicellulolytic and holocellulolytic chimeras Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-12 P. Monica, Ritesh Ranjan, Mukesh Kapoor
To understand the influence of family 3 Carbohydrate Binding Module (hereafter CBM3), single (GH5 cellulase; CelB, CelBΔCBM), bi-chimeric [GH26 endo-mannanase (ManB-1601) and GH11 endo-xylanase (XynB); ManB-XynB [1], ManB-XynB-CBM] and tri-chimeric [ManB-XynB-CelB [1], ManB-XynB-CelBΔCBM] enzyme variants (fused or deleted of CBM) were produced and purified to homogeneity. CBM3 did not alter the pH
-
Recent advances in laccase activity assays: a crucial challenge for applications on complex substrates Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-10 Elise Martin, Pascal Dubessay, Eric Record, Fabrice Audonnet, Philippe Michaud
Despite being one of the first enzymes discovered in 1883, the determination of laccase activity remains a scientific challenge, and a barrier to the full use of laccase as a biocatalyst. Indeed, laccase, an oxidase of the blue multi-copper oxidases family, has a wide range of substrates including substituted phenols, aromatic amines and lignin-related compounds. Its one-electron mechanism requires
-
Functional study of a lytic polysaccharide monooxygenase MsLPMO3 from Morchella sextelata in the oxidative degradation of cellulose Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-10 Lei Ma, Mengmeng Wang, Ya Gao, Yinghong Wu, Chaoqiang Zhu, Shuyu An, Siyu Tang, Qiusheng She, Jianmin Gao, Xiaohui Meng
Lytic polysaccharide monooxygenases (LPMOs) can improve the effectiveness with which agricultural waste is utilized. This study described the potent AA9 family protein MsLPMO3, derived from Morchella sextelata. It exhibited strong binding to phosphoric acid swollen cellulose (PASC), and had the considerable binding ability to Cu2+ with a Kd value of 2.70 μM by isothermal titration calorimetry (ITC)
-
Mutational analysis of predicted active site residues of an exoglucanase secreted by Xanthomonas oryzae pv. oryzae to determine their role in catalysis and in virulence on rice Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-06 Lavanya Tayi, Rajkanwar Nathawat, Sushil Kumar, Roshan V. Maku, Hitendra Kumar Patel, Rajan Sankaranarayanan, Ramesh V. Sonti
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice. As a part of its virulence repertoire, Xoo secretes a cell wall degrading enzyme Cellobiosidase (CbsA), which is a critical virulence factor and also a determinant of tissue specificity. CbsA protein is made up of an N-terminal catalytic domain and a C-terminal fibronectin type III domain. According to the CAZy classification
-
Interchangeable modular design and operation of photo-bioreactors for Chlorella vulgaris cultivation towards a zero-waste biorefinery Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-12-05 Ombretta Paladino, Matteo Neviani
-
Production of octyl butyrate using psychrophilic mutant lipase from Croceibacter atlanticus LipCA lipase developed by a molecular evolution technique Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-30 Ha Min Song, Hyung Kwoun Kim
-
A GH19 lysozyme and peptidase from Myoviridae cyanophages lacking the typical holin-endolysin system exhibit lytic activity Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-28 Li-Hui Meng, Fei Ke, Qi-Ya Zhang, Ya-Dong Hu, Zhe Zhao
Most of the dsDNA cyanophages employ holin-endolysin lysis systems to damage the host cells. This study aimed to elucidate the lytic activity of ORF91 and ORF117 in the cyanophage MaMV-DH01, which lacked a conventional cholinesterase system. These two proteins contained Lyz-like superfamily domains and were annotated as a member of GH family 19 (named DHGH19) and peptidase (named DHpeptidase), respectively
-
Environmental pollution removal using electrostimulation of microorganisms by alternative current Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-29 Shabnam Ahmadi, Abbas Rezaee
-
Improved titer and stability of selenium nanoparticles produced by engineered Saccharomyces cerevisiae Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-28 Jie Sun, Yi Wang, Yixuan Zheng, Mengjie Yuan, Hangjun Zhang, Guangliang Huo, Ming Weng, Ruicheng Jiang, Yinjun Zhang, Yuguang Wang
Selenium nanoparticles (SeNPs) have gained significant attention in the fields of medicine and healthcare products due to their various biological activities and low toxicity. In this study, we focused on genetically modifying the Saccharomyces cerevisiae strain YW16 (CICC 1406), which has the ability to efficiently reduce sodium selenite and produce red SeNPs. By overexpressing genes involved in glutathione
-
Identification of bacterial protease domains that cleave human IgM Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-25 Tricia Windgassen, Nikki Kruse, Brian Ferrer, Faye Du, Hirdesh Kumar, Adam P. Silverman
Immunoglobulin-degrading proteases are secreted by pathogenic bacteria to weaken the host immune response, contributing to immune evasion mechanisms during an infection. Proteases specific to IgG and IgA immunoglobulin classes have previously been identified and characterized, and only a single report exists on a porcine specific IgM-degrading enzyme. It is unclear whether human pathogens also produce
-
Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-22 Murillo Peterlini Tavares, Túlio Morgan, Riziane Ferreira Gomes, Jean Pierre Rocha Mendes, William Castro-Borges, Gabriela Piccolo Maitan-Alfenas, Valéria Monteze Guimarães
The phytopathogenic fungus Chrysoporthe cubensis is a relevant source of lignocellulolytic enzymes. This work aimed to compare the profile of lignocellulose-degrading proteins secreted by C. cubensis grown under semi-solid state fermentation using wheat bran (WB) and sugarcane bagasse (SB). The exoproteomes of the fungus grown in wheat bran (WBE) and sugarcane bagasse (SBE) were qualitative and quantitatively
-
Exploring the diversity of β-glucosidase: Classification, catalytic mechanism, molecular characteristics, kinetic models, and applications Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-22 Mehmet Emre Erkanli, Khalid El-Halabi, Jin Ryoun Kim
High-value chemicals and energy-related products can be produced from biomass. Biorefinery technology offers a sustainable and cost-effective method for this high-value conversion. β-glucosidase is one of the key enzymes in biorefinery processes, catalyzing the production of glucose from aryl-glycosides and cello-oligosaccharides via the hydrolysis of β-glycosidic bonds. Although β-glucosidase plays
-
Sequential and enzyme-assisted extraction of algal bioproducts from Ecklonia maxima Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-22 Blessing Mabate, Brett Ivan Pletschke
Brown algae are gaining recognition as sources of bio-compounds with diverse properties and potential applications in the food, nutraceutical, and pharmaceutical industries. Compounds such as polyphenols, alginates and fucoidan possess multiple bioactivities, including antidiabetic, antioxidant, anticancer, anti-inflammatory, and antibacterial properties. Conventional extraction methods provide low
-
Characterization of a novel recombinant D-mannose isomerase from Bifidobacterium bifidum and its catalytic mechanism Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-08 Shuang Xiao, Xiaoqiao Luo, Peng Zhang, Guowen Zhang, Xing Hu
-
DNA flexible chain modified MOFs as a versatile platform for chemoenzymatic cascade reactions in glucose catalysis Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-07 Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
-
Potential utility of bacterial protein nanoreactor for sustainable in-situ biocatalysis in wide range of bioprocess conditions Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-07 Yashodhara D. Shinde, Chiranjit Chowdhury
-
Enzymatic degradation of poly(ethylene terephthalate) (PET): Identifying the cause of the hypersensitive enzyme kinetic response to increased PET crystallinity Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-11-04 Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
-
Effects of electrospun nanofiber fabrications on immobilization of recombinant Escherichia coli for production of xylitol from glucose Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-29 Norhamiza Mohamad Sukri, Nor Hasmaliana Abdul Manas, Nardiah Rizwana Jaafar, Roshanida A. Rahman, Abdul Munir Abdul Murad, Rosli Md Illias
-
Functional characterization of a naphthalene-O-methyltransferase from Nocardia sp. CS682 Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-28 Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Methylation plays important roles in biosynthesis, metabolism, signal transduction, detoxification, protein sorting and repair, and nucleic acid processing. Generally the methyltransferases transfer methyl groups in various natural products using S-adenosyl methionine (SAM) as a cofactor. In this study, we examined and functionally characterized ThnM3 (enzyme), by testing various substrates with different
-
Using directed evolution to improve hydrogen production in chimeric hydrogenases from algal species Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-27 Scott M. Plummer, Mark A. Plummer, Patricia A. Merkel, Lisa A. Waidner
Algae generate hydrogen from sunlight and water utilizing high-energy electrons generated during photosynthesis. The amount of hydrogen produced in heterologous expression of the wild-type hydrogenase is currently insufficient for industrial applications. One approach to improve hydrogen yields is through directed evolution of the DNA of the native hydrogenase. Here, we created 113 chimeric algal hydrogenase
-
Chondroitinase as a therapeutic enzyme: Prospects and challenges Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-21 Asma Kheirollahi, Solmaz Sadeghi, Shirin Orandi, Kiana Moayedi, Khosro Khajeh, Mehdi Khoobi, Abolfazl Golestani
The chondroitinases (Chase) are bacterial lyases that specifically digest chondroitin sulfate and/or dermatan sulfate glycosaminoglycans via a β-elimination reaction and generate unsaturated disaccharides. In recent decades, these enzymes have attracted the attention of many researchers due to their potential applications in various aspects of medicine from the treatment of spinal cord injury to use
-
Comparison of hydrothermolysis and mild-alkaline pretreatment methods on enhancing succinic acid production from hydrolyzed corn fiber Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-16 Lisbeth Vallecilla Yepez, Boanerges Bamaca Saquic, Mark R. Wilkins
In the present work, mild alkaline pretreatments using either sodium hydroxide (0.05 g/g corn fiber) or calcium hydroxide (lime) (0.05 g/g corn fiber) were optimized and compared with hydrothermolysis pretreatment to enhance bioproduction of succinic acid from hydrolyzed corn fiber. The concentration, yield, and productivity of succinic acid from sodium hydroxide corn fiber hydrolysate (SH-CFH) were
-
An NAD+-dependent group Ⅲ alcohol dehydrogenase involved in long-chain alkane degradation in Acinetobacter venetianus RAG-1 Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-20 Shuai Chen, Jia Liu, Ge Gao, Mingchang Li, Lu Cao, Tongtong Liu, Guoqiang Li, Ting Ma
Alcohol dehydrogenases (ADHs) are a class of key enzymes responsible for the oxidation of alkyl alcohols in the aerobic alkane metabolic pathway. Currently, the degradation mechanisms of short- and medium-chain alkanes are commonly reported, while those of long-chain alkanes have received less attention. In this work, a putative long-chain ADH was screened from Acinetobacter venetianus RAG-1 via RNA-seq
-
A UPLC-based activity assay method for aminoglycoside phosphotransferase 4-Ia (APH4): A Selectable marker of plant transformation Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-19 Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
-
Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-12 Taghreed Elkasaby, Dao Duy Hanh, Prihardi Kahar, Hideo Kawaguchi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino
-
Intensification of corn fiber saccharification using a tailor made enzymatic cocktail Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-17 Julen Ordeñana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
The transition from an economic model based on resource extraction to a more sustainable and circular economy requires the development of innovative methods to unlock the potential of raw materials such as lignocellulosic biomasses. Corn fiber differs from more traditional lignocellulosic biomasses due to its high starch content, which provides additional carbohydrates for fermentation-based biomanufacturing
-
Marine antifouling properties of enzyme modified polyaniline coated stainless steel surface Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-06 Handan Üstükarcı, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
-
Production of extracellular agarase from Priestia megaterium AT7 and evaluation on marine algae hydrolysis Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-05 Dang Thi Thanh Ha, Le Thi Kim Thoa, Trinh Thi Phuong Thao, Tran Trung Dung, Tran Thi Minh Ha, Truong Thi Phuong Lan, Kuan Shiong Khoo, Pau Loke Show, Nguyen Duc Huy
Agar is a common component biosynthesized from various marine algae species that is widely applied in various fields including food and pharmaceutical industries. However, the structural composition of agar is highly resisted against chemical and biological hydrolysis. Therefore, tremendous research is exploring various pretreatment strategies to break down the intrinsic chemical structural of agar
-
Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-10-04 Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Laccase is an efficient biocatalyst for oxidative polymerization of organic substrates. However, cost of enzyme preparation, low stability and residual protein diminish the efficiency of laccase mediated polymerization. In this work, a series of silicon dioxide coated ferroferric oxide magnetic nanoparticles were modified by different functional groups including γ-methacryloxypropyltrimethoxy, succinic
-
Production and characterization of novel thermostable CotA-laccase from Bacillus altitudinis SL7 and its application for lignin degradation Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-09-29 Sanam Islam Khan, Miray Sahinkaya, Dilsat Nigar Colak, Numan Saleh Zada, Ugur Uzuner, Ali Osman Belduz, Sabriye Çanakçi, Alam Zeb Khan, Samiullah Khan, Malik Badshah, Aamer Ali Shah
Laccases are multi-copper oxidases and found in ligninolytic bacteria catalyzing the oxidation of both phenolic and non-phenolic compounds, however its application in lignin degradation suffers due to low oxidation rate, which have intensified the search for new laccases. In the present study, spore coat A protein (CotA) encoding gene having laccase like activity from Bacillus altitudinis SL7 (CotA-SL7)
-
2-Hydroxyisovalerate production by Klebsiella pneumoniae Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-09-29 Qinghui Wang, Weiyan Jiang, Yaoyu Cai, Marina Tišma, Frank Baganz, Jiping Shi, Gary J. Lye, Wensheng Xiang, Jian Hao
2-Hydroxyisovalerate is a valuable chemical that can be used in the production of biodegradable polyesters. In nature, it was only produced at a very low level by Lactococcus lactis. 2-Ketoisovalerate is an intermediate metabolite of the branched-chain amino acid biosynthesis pathway, and Klebsiella pneumoniae ΔbudAΔldhA (Kp ΔbudAΔldhA) was a 2-ketoisovalerate producing strain. In this research, 2-hydroxyisovalerate
-
Production of an O-desmethylated product, a major human metabolite, of rabeprazole sulfide by bacterial P450 enzymes Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-09-20 Ngoc Tan Cao, Gun Su Cha, Jeong-Hoon Kim, Yujin Lee, Chul-Ho Yun, Ngoc Anh Nguyen
-
Sustainability analysis of orange peel biorefineries Enzyme Microb. Technol. (IF 3.4) Pub Date : 2023-09-20 Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo José Inocencio-García, Carlos Ariel Cardona Alzate
Biorefineries are constantly evolving since new technological advances in enzyme and microbial processes are boosting research for producing new bio-based products. Nevertheless, the step towards real process implementation must overcome a series of stages based on process sustainability in the early design stages. Orange peel (OP) has been profiled as a potential raw material for producing different