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A 1.6 Mb region on SSC2 is associated with antibody response to classical swine fever vaccination in a mixed pig population Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-15 Arnav Mehrotra; Bharat Bhushan; Amit Kumar; Manjit Panigrahi; Karthikeyan A.; Akansha Singh; Ashok K. Tiwari; Hubert Pausch; Triveni Dutt; Bishnu P. Mishra
Abstract Classical Swine Fever (CSF) is a contagious viral disease of pigs which is endemic in several parts of the world, including India. Prophylactic vaccination using live attenuated vaccine is the preferred method of control. However, there is significant inter-individual variation in the antibody response to vaccination. In this study, we measured the E2 antibody blocking percentage after 21 days
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Evaluation of genetic diversity and population structure in four indigenous duck breeds in Vietnam Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-15 Lan Doan Pham; Duy Ngoc Do; Le Quang Nam; Nguyen Van Ba; Pham Hai Ninh; Doan Phuong Thuy; Pham Van Son; Pham Cong Thieu
Abstract This study characterized genetic diversity and population structure of four indigenous Vietnamese duck breeds and an exotic breed for setting the conservation priority. A total of 200 samples from four duck breeds (Sincheng, Minhhuong, Muongchieng and Bauben) and an exotic breed (Supermeat) were genotyped for fifteen microsatellite markers. The average number of alleles per locus was 14.07
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Complete mitogenome of Treron sphenurus (Aves, Columbiformes): the first representative from the genus Treron, genomic comparisons and phylogenetic analysis of Columbidae Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-13 Yun-Xia Chen; Cheng-He Sun; Yao-Kai Li; Yi-Ling Fei; Xiao-Ming Xue; Sen-Lin Hou; Yong-Wu Zhou; Jing Jiang; Hai-Tao Guo
Abstract The wedge-tailed green pigeon (Treron sphenurus) has a protective value in the evolution of the family Columbidae. In this study, the complete mitogenome of T. sphenurus from Baise City, China, which represents the first sequenced species of the genus Treron in Tribe Treronini, is reported. This was accomplished using PCR-based methods and a primer-walking sequencing strategy with genus-specific
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A variant associated to IGF-1 mRNA and protein expression in sheep Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-11 Luis A. Flores-Encinas; Felipe A. Rodríguez-Almeida; Monserrath Felix-Portillo; Francisco J. Jahuey-Martínez; José A. Martínez-Quintana
Abstract The insulin-like growth factor-1 (IGF-1), a key hormone in muscle development was investigated for single-nucleotide polymorphisms (SNPs) upstream of the IGF-1 gene and their effects upon its cognate mRNA and hormone levels in sheep. A 70 d feeding trial was conducted with 22 F1 (Dorper × Pelibuey) lambs, individually allocated and fed a diet with a forage-to-concentrate ratio of 36:64 and
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Identification of novel single-nucleotide polymorphism at exon1 and 2 region of B4GALT1 gene and its association with milk production traits in crossbred cattle of Kerala, India Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Jamuna Valsalan; Tina Sadan; Thirupathy Venkatachalapathy; Kulangara Anilkumar; Thazhathu Veettil Aravindakshan
Abstract Beta 1,4-galactosyltransferase-I gene (B4GALT1) is an important candidate gene for milk performance traits, encodes catalytic part of lactose synthesis. Main objectives of present study is identification of single-nucleotide polymorphism in exon 1 and 2 region of B4GALT1 and to find significant association of genetic variants with milk performance traits in crossbred cattle of Kerala. The
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AFLP markers based genetic diversity and population structure analysis of Kadaknath: an indigenous black meat poultry breed of India Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Shashi Bhushan Tripathi; Gayaprasad Jatav; Anoop Anand Malik; Shrikant Joshi; Vivek Kumar Singh; Madan Singh Negi; Laxmi Chauhan; Shyam Sundar Sharma
Abstract The current study is the first worldwide to assess the genetic diversity of Kadaknath poultry using amplified fragment length polymorphism (AFLP) markers. Out of total 96 accessions, four were outliers and 92 were Kadaknath accessions of which 30 were males, 62 were females. Of these, 74 were jet black, 7 penciled and 11 were golden feather color type of Kadaknath. High level of polymorphism
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Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Syed Atif Ali; Nongthombam Boby; Prasanna Preena; Shiv Varan Singh; Gurpreet Kaur; Subrata Kumar Ghosh; Sukdeb Nandi; Pallab Chaudhuri
Abstract A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were mixed in bovine semen samples. The µcLAMP assay is portable, user-friendly
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Correction Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10
(2021). Correction. Animal Biotechnology. Ahead of Print.
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Influences of vitamin A, L-carnitine, and folic acid in ovo feeding on embryo and hatchling characteristics and general health status in ducks Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-07 Ahmed Gouda; Samar A. Tolba; Khalid M. Mahrose
Abstract The current investigation was conducted to test the potential effects of in ovo feeding of vitamin A, L-carnitine, and folic acid on embryonic growth and post-hatch performance. A total of 450 fertile duck eggs were randomly distributed into two experiments of five groups/experiment (255 eggs/experiment and 45 egg/group). The experimental groups were: negative control (non-injected eggs),
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Identification of three new microsatellites and their effects on body measurement traits in pigs using time of flight-mass spectrometry (TOF-MS) Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-05 Yanghai Zhang; Xuelian Zhang; Xingxing Xue; Wenjuan Shen; Lei Wang; Yuhong Ma; Jiping Zhou; Guofang Wu; Chuanying Pan
Abstract The body status of livestock affects their physiological function and productive performances. Microsatellites, one of the most used DNA markers, have been found to be associated with pig productive traits. However, their identifications and effects on body measurement traits of the Chinese Qinghai Bamei pig still uncovered. According to our previous sequencing data, in this study, three novel
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Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-30 Fernanda Borges Duarte; Marcelo de Macedo Brígido; Eduardo de Oliveira Melo; Sônia Nair Báo; Carlos Frederico Martins
Abstract Cells from different origins behave differently regarding the incorporation of exogenous DNA and formation of transgenic cells. Milk production of recombinant antibody may benefit from efficient transfection protocols to produce transgenic animals. In this context, the objective of this study was to verify the transfection potential of bovine mesenchymal stem cells from Wharton’s jelly (MSC-WJ)
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Detecting fecal egg count (FEC) for gastrointestinal nematodes of adult Turkish sheep with different scrapie related PRNP haplotypes Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-23 Yalçın Yaman; Bayram Şenlik; Mehmet Özüiçli; Murat Keleş; Ramazan Aymaz; Veysel Bay; Ecem Hatipoğlu; Seyrani Koncagül; Yasemin Öner; Cemal Ün
Abstract Scrapie is a transmissible spongiform encephalopathy caused by prions and leads to neurodegeneration in the Central Nervous System (CNS) of sheep and goats. Genetic resistance/susceptibility to scrapie is well studied and it is known that the variations of 136th, 154th and 171st codons at the ovine PRNP gene have a major effect on the development of the disease. Many studies demonstrated that
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Characterization and phylogenetic evolution of mitochondrial genome in Tibetan chicken Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Xiaoxu Jia; Junxian Lu; Xiujun Tang; Yanfeng Fan; Qinglian Ge; Yushi Gao
Abstract The objective of this study was to characterize mitochondrial genome and investigate phylogenetic evolution in Tibetan chicken. In this study, four haplotypes were identified based on D-loop sequencing in Tibetan chicken (n = 40), and each representative of four haplotypes was selected for total mitochondrial genome sequencing and analyzed together with published mitochondrial genome data
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Identification of a promoter polymorphism affecting GPAT3 gene expression that is likely related to intramuscular fat content in pigs Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Cai Ma; Yi Sun; Jiying Wang; Li Kang; Yunliang Jiang
Abstract The intramuscular fat content (IMF) is an economically important trait in pigs and the Laiwu pig is famous for its excessively extremely high level of IMF. Our previous transcriptome study revealed that the dynamic expression of glycerol-phosphate acyltransferase 3 (GPAT3) is consistent with changes in the IMF of Laiwu pigs. In this study, we further analyzed the expression and polymorphism
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Seasonal pattern in occurrence of rotavirus infection (RV) in diarrheic children, calves and piglets from Bareilly, India Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Bilal Ahmad Malla; Zunjar Baburao Dubal; Anukampa Kadwalia; Gazanfar Abass; Obli Rajendran Vinodh Kumar; Ashok Kumar; Kaushal Kishore Rajak; Ishfaq Maqbool; Aquil Mohmad; Vivekanandhan Rangaraju; Arfa Fayaz
Abstract Rapid and reliable diagnosis for diarrhoeal disease is critically important for the differentiation of etiological agents and subsequent suitable treatment modalities. The objective of the study is to reveal the seasonal pattern in the occurrence of rotavirus in diarrheic children, calves and piglets from Bareilly, Uttar Pradesh, India. A total of 115 diarrhoeal samples were collected, out
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Whole-genome resequencing reveals aberrant autosomal SNPs affect chicken feathering rate Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Mohan Qiu; Chaowu Yang; Huarui Du; Qingyun Li; Zengrong Zhang; Xia Xiong; Chunlin Yu; Xiaoyan Song; Chenming Hu; Bo Xia; Li Yang; Han Peng; Lan Liu; Xiaosong Jiang
Abstract Previous studies have shown that the feather growth rate of chicks is determined by two alleles located on the sex chromosome Z; however, in chicken production, feathering is usually not consistently controlled by the sex chromosome. To identify whether the feathering rate is related to autosomal inheritance, whole-genome resequencing was performed in eight chickens with slow- and fast-feathering
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Identification, biogenesis and function prediction of novel circRNA during the chicken ALV-J infection Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-16 Ting Yang; Lingling Qiu; Meng Bai; Laidi Wang; Xiaodan Hu; Lan Huang; Guohong Chen; Guobin Chang
Abstract Circular RNA (circRNA) is a new non-coding RNA with a highly conserved and stable covalently closed loop structure, and it plays an important role in a variety of biological processes and the occurrence of diseases. Based on the sequencing results, circRNA_3079 had the most significant difference between the infected group and normal group, up to about 8 times. It has attracted our attention
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Effects of different levels of dietary black cumin (Nigella sativa L.) and fenugreek (Trigonella foenum-graecum L.) and their combination on productive traits, selected blood constituents, microbiota and immunity of broilers Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-16 Vito Laudadio; Mohsen Nasiri-Dehbaneh; Rana Muhammad Bilal; Ali Qotbi; Faramin Javandel; Abbas Ebrahimi; Alireza Seidavi; Marina Slozhenkina; Ivan Gorlov; Peter G. Dunne; Vincenzo Tufarelli
Abstract The effects of inclusion of powdered seeds of black cumin (B) (Nigella sativa L.) and fenugreek (F) (Trigonella foenum-graecum L.) on productive traits, selected blood constituents, microbiota and immunity of broilers were studied. A total of 648 day-old chicks were randomly assigned to nine treatments, with four pen replicates, each with 18 birds, including three levels of B seed powder (BSP;
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A molecular marker of milk composition traits in NCAM2 gene of Chinese Holstein Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-14 Xiaoting Ding; Zijing Zhang; Ruifang Hu; Yifan Wen; Yongzhen Huang; Qiaoting Shi; Yajie Feng; Eryao Wang; Chuzhao Lei; Hua He
Abstract The aim of this study was to detect the novel copy number variation (CNV) locus of NCAM2 gene in Chinese Holstein, and to analyze the effect of the novel CNV locus in NCAM2 gene on milk composition traits. The novel CNV locus of NCAM2 gene in 310 Chinese Holstein was detected by real-time quantitative fluorescent PCR (qPCR) and association analysis was performed between the novel CNV locus
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Effects of sea buckthorn puree on risk factors of cardiovascular disease in hypercholesterolemia population: a double-blind, randomized, placebo-controlled trial Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-10 Fangfei Zhou; Jian Zhang; Ai Zhao; Yumei Zhang; Peiyu Wang
Abstract To investigate the effect of sea buckthorn puree consumption on reducing blood lipid and other risk factors of CVD. A total of 111 patients with hypercholesteromia who were treated with 90 ml sea buckthorn puree or placebo for 90 days were enrolled in this trial. Physical examination and analysis of lipid markers, hsCRP concentrations and cell adhesion protein concentrations with fasting blood
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Analysis of single nucleotide polymorphisms related to heifer fertility in Hanwoo (Korean cattle) Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-07 Seung-Hwan Lee; Dooho Lee; Mingyung Lee; Seung-Heui Ryoo; Seongwon Seo; Inchul Choi
Abstract Genome-wide association studies (GWAS) have accelerated the identification of functional trait loci in cattle and identified single nucleotide polymorphisms (SNPs) in candidate genes associated with fertility and production traits in high milk yield dairy cattle. The fertility of Hanwoo (Korean native beef cattle) has declined after the adaptation of a selection program for high quantity and
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Derivation and characterization of putative embryonic stem cells isolated from blastoderms of Taiwan Country chicken for the production of chimeric chickens Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-02 Chalothon Amporn; Pin-Chi Tang; Chien-Kai Wang
Abstract The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed’s adaptability to the area and disease resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regenerative medicine and species conservation,
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Preparation and characterization of titanium dioxide nanoparticles and in vitro investigation of their cytotoxicity and antibacterial activity against Staphylococcus aureus and Escherichia coli Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-28 Salim Albukhaty; L. Al-Bayati; H. Al-Karagoly; S. Al-Musawi
Abstract Titanium dioxide nanoparticles (TiO2 NPs) are photo-active metallic nanoparticles becoming promising agents in modern biomedical applications. Herein, a novel process for the synthesis of TiO2 NPs with high stability was developed by a sol–gel process and to investigate their cytotoxicity and antibacterial activity. Numerous experiments have been performed to confirm the morphologies, compositions
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Fish tyrosinase enzyme involved in melanin biosynthesis: Insights from physicochemical characterization, homology modeling, and virtual screening studies Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-26 Riya Kumari; Shrinivas Jahageerdar; Archana N Panche; Sanath Kumar
Abstract In the vertebrates, including fish, the tyrosinase enzyme plays an essential role in coloration. Modulation of tyrosinase activity is expected to alter the body pigmentation in fish and other vertebrate species. In the present study, physicochemical, functional, and structural properties of tyrosinase of three fish species viz., goldfish, Japanese medaka, and common carp were determined. The
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Effect of indel variants within the sorting nexin 29 (SNX29) gene on growth traits of goats Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-19 Yi Bi; Yuhan Chen; Dongyun Xin; Tingting Liu; Libang He; Yuxin Kang; Chuanying Pan; Weijun Shen; Xianyong Lan; Mei Liu
Abstract The sorting nexin 29 gene (SNX29) is a well-known regulator of myocyte differentiation and proliferation. In this work, two indels (17-bp and 21-bp) were identified in the goat SNX29 gene, and their effects on the growth traits of 1,759 Shaanbei white cashmere (SBWC) goats were analyzed. Both indels had three genotypes [homozygote wild type (II), heterozygote (ID), and homozygote mutation
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Effect of dietary supplementation of wildCumin (Bunium persicum) seeds on performance, nutrient digestibility and circulating metabolites in broiler chicks during the finisher phase Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-11 Muhammad Shuaib; Nasr Ullah; Abdul Hafeez; Ibrahim A. Alhidary; Mutassim M. Abdelrahman; Rifat Ullah Khan
Abstract The objective of the present study was to evaluate the impact of inclusion of wild cumin seeds (WCS) on performance, nutrient digestibility and blood profile in broilers during the finisher phase. For this purpose, 360, 14 days old chicks were randomly divided into four groups designated as CONT (control), 0.5WCS, 1.0WCS and 1.5WCS with 5 replicates, which were supplemented with WCS at the
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Development of TaqMan PCR assay for detection of A and B variants of the bovine β-lactoglobulin Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-11 Svetlana N. Kovalchuk; Anna L. Arkhipova
Abstract β-Lactoglobulin (BLG) is one of the prevalent whey protein in cattle. To date, several variants of bovine BLG have been found, but the most common are A and B, which differ from each other by SNPs rs109625649 and rs110066229. Numerous studies showed effects of A and B variants of BLG on milk yield, fat and protein content and cheese-making properties. To date, polymerase chain reaction-restriction
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Expression and polymorphism of Follistatin (FST) gene and its association with growth traits in native and exotic chicken Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-10 K. Dushyanth; R. Shukla; R.N. Chatterjee; T. K. Bhattacharya
Abstract Follistatin (FST), a member of the transforming growth factor beta super-family regulates body growth by inhibiting the binding of myostatin (an inhibitor of growth) with its receptor in chicken. An experiment was conducted to explore ontogenic expression of the follistatin gene, determine polymorphism at the coding region of the gene and estimate its effect on growth traits in native (Aseel)
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Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-24 İbrahim Aytekin,Mervan Bayraktar,Çağrı Melikşah Sakar,İlker Ünal
Abstract This study aims to find the association between MYLK4 gene polymorphism and growth traits at different ages, such as birth, 3rd-month, 6th-month and 12th-month ages in Anatolian black cattle. PCR-RFLP method was used to detect the polymorphism for MYLK4 gene in at G61595A locus. Allele and genotype frequencies were A: 0.34, G: 0.66 and AA: 0.17, AG: 0.34 and GG: 0.49. The chi-square χ2 test
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Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-08 Chengjian Zhou; Ping Li; Meihong Han; Xuejun Gao
Abstract Fat deposition in skeletal muscle is an important aspect of improving meat quality. Isoflavones can promote animal anabolism, but whether and how they regulate muscle fat deposition remain largely unclear. In this study, we explored the role and corresponding molecular mechanism of one of the major isoflavones, daidzein, in fat deposition in C2C12 myoblast cells. In the absence of fatty acids
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Oral administration of potato peel extract affects serum blood metabolites, liver function and ameliorating oxidative stress induced in rabbits exposed to cold stress Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-08 Yassmine M. El-Gindy; Salma H. Abo-Hafsa; Moyosore J. Adegbeye; Abdelfattah Z. M. Salem; Waleed M. Dosoky
Abstract This study investigated the effect of potato peel extract (PPE), orally administrated to rabbits, on serum blood metabolites and ameliorating oxidative stress induced by cold stress under Egyptian winter conditions. Twenty-four bucks grouped into three treatments (8 animals per group) were used for the experiment. The animals received 1.5 ml of water orally, containing 0 (PPE0), 25 (PPE25)
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A nonsynonymous SNP within the AMH gene is associated with litter size in Dazu black goats Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 Yan-Guo Han; Yan Zeng; Yong-Fu Huang; De-Li Huang; Peng Peng; Ri-Su Na
Abstract AMH, KISS1R and GDF9 genes play a vital role in human and animal reproduction and might be used as the genetic markers for the reproduction traits selection. The aim of this study was to screen the single nucleotide polymorphisms (SNPs) within the AMH, KISS1R and GDF9 genes and to determine the correlations between these SNPs and the litter size in goats. Nine single SNPs within these genes
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Transcriptional regulation of adipogenic marker genes for the improvement of intramuscular fat in Qinchuan beef cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 Guo Hongfang; Rajwali Khan; Sayed Haidar Abbas Raza; Kaster Nurgulsim; Syed Muhammad Suhail; Abdur Rahman; Ijaz Ahmed; Asim Ijaz; Iftikhar Ahmad; Zan Linsen
Abstract The intramuscular fat content plays a crucial role in meat quality traits. Increasing the degree of adipogenesis in beef cattle leads to an increase in the content of intramuscular fat. Adipogenesis a complex biochemical process which is under firm genetic control. Over the last three decades, the Qinchuan beef cattle have been extensively studied for the improvement of meat production and
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Identification of important miRNAs in bubaline mammary gland at heifer stage - An in-silico approach Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 S. M. K. Thirumaran; Manjit Panigrahi; Sheikh Firdous Ahmad; Triveni Dutt; Bharat Bhushan
Abstract The present study was undertaken to identify the microRNAs (miRNAs) expressed in the mammary tissue of a bubaline heifer. Small RNAs were isolated from the mammary gland tissue and enriched for miRNA fraction. The linker-ligated small RNAs were reverse transcribed to synthesize cDNA and amplified by PCR. The PCR products were ligated to the pGEM-T Easy vector; cloned into DH5 alpha cells and
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A novel SNP of MYO1A gene associated with heat-tolerance in Chinese cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-04 Yanhong Cao; Peng Jia; Zhuyue Wu; Mingguang Huang; Shaomei Chen; Jicai Zhang; Bizhi Huang; Chuzhao Lei
Abstract With the advent of global climate change, heat-tolerance is becoming more and more important to the sustainability of animal husbandry production systems. Previous studies have shown that MYO1A gene associated with pigmentation may be closely related to heat-tolerance in cattle. In this study, a novel missense mutation (NC_037332.1 g.56390345 A > G) was first detected in MYO1A in 891 individuals
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Characterization of the promoter region of bovine ATP5B: roles of MyoD and GATA1 in the regulation of basal transcription Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-30 Zhidong Zhao; Sayed Haidar Abbas Raza; Yuzhu Luo; Jiqing Wang; Xiu Liu; Shaobin Li; Bingang Shi; Jiang Hu
Abstract Intramuscular fat (IMF) content is a key determinant of beef quality, making it a key topic of research interest. ATP5B serves as the catalytic component of the mitochondrial ATP synthase complex and plays essential roles in controlling fat contents and oxidative metabolism in bovine skeletal muscle. In this study, we determined that bovine ATP5B was highly expressed in longissimus thoracis
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Significance of molecular chaperones and micro RNAs in acquisition of thermo-tolerance in dairy cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-29 S. R. Mishra
Abstract Ambient temperature is considered as the major abiotic factor which regulates body physiological mechanisms of all living creatures across the globe. Variation in ambient temperature which emulates thermoneutral zone culminates in heat stress. Heat stress has been emerged as major ultimatum to livestock’s growth, development, production and reproduction across the world. Livestock’s responds
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Phylogenetic differentiation between Awassi and Hamdani sheep using the mitochondrial 12S rRNA Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-28 Israa A. Fadhil; Mohammed Baqur S. Al-Shuhaib
Abstract This study was conducted to assess the role of the mitochondrial 12S rRNA variations in the phylogenetic discrimination between two Iraqi breeds of sheep that differ in geographical distribution. A total of 122 animals (68 Awassi and 54 Hamdani) were included in the study. Direct sequencing of amplicons followed by the construction of a median-joining network and several trees were performed
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The use of cold pressed oils as eco-friendly alternatives for antibiotics in high and low-CP diets of laying Japanese quail Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-23 Mahmoud Alagawany; Mohamed M. El-Hindawy; Laila A. Mohamed; Rana M. Bilal; Jamila Soomro
Abstract A trial was conducted to access the impact of varying levels of dietary protein (CP) and cold-pressed oil on hematological parameters, liver and kidney function, antioxidant and immunoglobulin indices and lipid profile of Japanese quail at the laying period. A number of 324 mature ten-week of age Japanese quails (216 females and 108 males) were selected. The red blood cells were increased
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Cloning and characterization of linker histone H1 gene in rohu, Labeo rohita Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-16 Sonali Parida; Amruta Mohapatra; Sweta Das; Pramoda Kumar Sahoo
Abstract Linker histone H1 (LHH1) is an abundant nuclear protein that condenses chromatin to form higher-order structure. The present study reported cloning and sequencing of 942 bp of LHH1 from liver tissue of rohu, Labeo rohita, with a complete coding sequence of 792 bp of having 263 amino acids. The phylogenetic tree of L. rohita LHH1 (LrLHH1) shared maximum similarity with that of Carassius auratus
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The distribution of four missense mutations in SPTBN5 gene across native Chinese breeds Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-16 Hongchao Zhou; Bizhi Huang; Kaixing Qu; Jianyong Liu; Jicai Zhang; Chuzhao Lei
Abstract Modern cattle belong to two subspecies, Bos taurus and Bos indicus. Since divergence, cattle types have accumulated different genetic variations, which have contributed to highly differentiated phenotypes. The mammalian inner ear possesses functional and morphological innovations that contribute to its unique hearing capacities. The spectrin beta, non-erythrocytic 5 (SPTBN5) gene has been
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Assessment of genetic diversity of Cervus albirostris (white-lipped deer) by microsatellite markers Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-15 Ruobing Han; Hongnan Zhao; Defa Li; Heping Li
Abstract Cervus albirostris (white-lipped deer) is a unique plateau deer in China. In order to conserve the white-lipped deer population and maintain its sustainable development in Liaoyang, China, ten microsatellite markers was used to analyze the genetic diversity of 42 white-lipped deer, an ex-situ conservation population from Yushu (Qinghai) to Liaoyang (Liaoning). The results showed that expected
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Relationships between novel nucleotide variants within the colony-stimulating factor 1 receptor (CSF1R) gene and mastitis indicators in sheep Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-12 Huina Hu; Yuta Yang; Xianyong Lan; Qingfeng Zhang; Chuanying Pan
Abstract Colony-stimulating factor 1 receptor (CSF1R) plays an important role in the process of innate immunity and inflammation, thus it was hypothesized that the CSF1R gene might affect the occurrence of mammalian mastitis. The purpose of this study was to investigate the association between nucleotide variations of CSF1R gene and mastitis in Australian white sheep (AUWs). Two indel variants (Intron5-27 bp
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Characterization and expression of MUSTN1 gene from different duck breeds Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-09 Zhixiu Wang; Wenshuang Liang; Xiangxiang Li; Yang Zhang; Qi Xu; Guohong Chen; Hao Zhang; Guobin Chang
Abstract Muscle growth rate and muscle mass are important economic traits in animal production. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) gene has been implicated in myofusion as well as skeletal muscle growth and repair; however, the exact role and expression of MUSTN1 in different duck breeds are not fully understood. To gain insights into the biological functions of MUSTN1 in skeletal
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Determining Theileria annulata parasitemia through qPCR in lactating cows of Odisha, − a theileriosis-endemic region of India Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-07 Sonali Sahoo; Niranjana Sahoo; Sangram Biswal; Bijayendranath Mohanty; Bikash Behera; Avishek Pahari; Kashyap Bhuyan
Abstract Study was undertaken in a theileriosis-endemic region of India during May 2018 to April 2019 among milch cows. Blood samples collected from apparently healthy (n = 65) and Theileria-suspect cows (n = 65) were screened against T. annulata and T. orientalis infection by SYBR Green‒based real time PCR using primers designed from the isolates of study area. Cows having single infection with T
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Metabolome fingerprints, performance and carcass quality of beef calves supplemented with antibiotic free additive Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-06 Jefferson R. Gandra; Cibeli A. Pedrini; Nara R. B. Cônsolo; Anderson P. Acosta; Leonardo O. Seno; Luis C. G. S. Barbosa; Isabelle Z. Noia; Vicente L. M. Buarque; Angel R. H. Padilla; Luis A. Colnago; Erika R. S. Gandra
Abstract The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group that received the basal creep feeding diet and PRO animals that received the
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Evaluation of a panel of microsatellite markers to study their applications in Serrapinnus notomelas and to reveal the genetic diversity in Hyphessobrycon eques Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-05 Andrei Lincoln Yamachita; Victor César Freitas Pandolfi; Felipe Pinheiro de Souza; Sara Mataroli de Godoy; Ed Christian Suzuki de Lima; Ulisses de Pádua Pereira; Jayme Aparecido Povh; Eric Costa Campos; Denise Rocha Ayres; Annaiza Braga Bignardi; Claudete de Fátima Ruas; Nelson Mauricio Lopera-Barrero
Abstract Jewel tetra (Hyphessobrycon eques) is a freshwater fish found in several rivers and basins in South America. The present study is the first study to create a panel of microsatellite markers for detecting genetic diversity in H. eques and evaluating the application of these markers in Serrapinnus notomelas. In total, 44 individuals were genotyped from the natural (WIL, n = 20) and stock in
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Effect of different levels of pomegranate peel powder and probiotic supplementation on growth, carcass traits, blood serum metabolites, antioxidant status and meat quality of broilers Anim. Biotechnol. (IF 1.487) Pub Date : 2020-10-01 Shahira Abdel Baset; Elwy A. Ashour; Mohamed E. Abd El-Hack; Mohamed M. El-Mekkawy
Abstract The present study aimed to investigate the effect of dietary pomegranate peel powder (PPP) and probiotic bacteria (PB) on the growth rate, carcass traits, blood serum metabolites, and meat quality of Ross broiler chickens during 1–5 weeks of age. A total of 480 unsexed Ross broiler chicks 7-days old with the beginning bodyweight of 110.58 ± 0.17 g were employed in a complete randomized purpose
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Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case–control study in the Indian cattle population Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-28 Satish Kumar; Subodh Kumar; Ran Vir Singh; Anuj Chauhan; Amit Kumar; Jaya Bharati; Shoor Vir Singh
Abstract This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical body condition score, which was then screened by a panel of diagnostic tests
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Association between ovine Toll-like receptor 4 (TLR4) gene coding variants and presence of Eimeria spp. in naturally infected adult Turkish native sheep. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-24 Yalçın Yaman,Ramazan Aymaz,Murat Keleş,Veysel Bay,Mehmet Özüiçli,Bayram Şenlik
Coccidiosis caused by Eimeria spp. is a protozoan disease prevalent in farm animals, and it is responsible for serious economic losses especially in young animals. It has been popular to breed disease-resistant animals due to the concern about food safety, animal welfare, and public health. Toll-like receptor (TLR) gene family plays a key role in the innate immune system participating in host-antigen
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Transcriptomic study of yak mammary gland tissue during lactation. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-22 Jinbo Wu,Shiming He,Zhonghua Yu,Daoliang Lan,Xianrong Xiong,Zhu Li
Yak milk, a high-quality milk, is one of the best raw materials for dairy products and economically important to pastoral herdsmen. To make a further understanding of the molecular differences in mammary tissues of the yaks with different milk production during lactation, in this study, we took the use of RNA-seq to perform high-throughput sequencing and analysis of the mammary gland transcriptomes
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Gallic acid as a key substance to inhibit proliferation and adipogenesis in bovine subcutaneous adipocyte. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-18 Qing Jin,Guifen Liu,Xiuwen Tan,Xianglun Zhang,Xiaomu Liu,Chen Wei
Gallic acid (GA) is a widespread naturally occurring phenolic acid and one of the main active monomers that forms polyphenols such as tannins. In recent years, GA has been found as a potential regulator in lipid metabolism. However, effects and possible mechanisms of GA on cell growth and lipid metabolism of bovine subcutaneous adipocytes remain unknown. In this study, we investigated whether GA could
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Traditional Chinese herbal medicine complex supplementation improves reproductive performance, serum biochemical parameters, and anti-oxidative capacity in periparturient dairy cows. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-15 Maoliang Ran,Cheng Cha,Youtao Xu,Hongliang Zhang,Zecao Yang,Zhicai Li,Shuilian Wang
This study was conducted to investigate the effects of a traditional Chinese herbal medicine complex (TCHMC) on the productive performance of periparturient dairy cows. Eighteen non-lactating pregnant Holstein dairy cows with similar body conditions with 1 to 2 parity were randomly divided into three groups (n = 6), receiving a basal diet with 0 (CON group), 200 (T-200 group), and 300 (T-300 group)
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Consequences of varying dietary crude protein and metabolizable energy levels on growth performance, carcass characteristics and biochemical parameters of growing geese. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-12 Mahmoud Alagawany,Elwy A Ashour,Mohamed S El-Kholy,Diaa E Abou-Kassem,Tamer Roshdy,Mohamed E Abd El-Hack
This research aimed to investigate the effect of varying dietary crude protein (CP) and metabolizable energy (ME) quantity on growth, carcass characteristics, biochemical blood parameters and molecular aspects of growing geese. Overall, a total of 180 Egyptian goslings were used in a 2 × 3 factorial design. Two levels of CP (18% and 16%) and three levels of ME (3000, 2900 and 2800 kcal/kg diet) were
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Isolation and propagation of classical swine fever virus in porcine Wharton's Jelly mesenchymal stem cells. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-04 Neelam R Tomar,Irfan A Bhat,Mukesh K Bharti,Jeny K John,Veena Sharma,Vikash Chandra,G Taru Sharma,G Saikumar
Classical Swine Fever (CSF) is an extremely infectious and deadly disease of pigs and wild boars caused by the CSF virus (CSFV) which is a member of the Pestivirus genus and the family Flaviviridae. This study was designed to detect the permissibility and replication of CSFV in mesenchymal stem cells (MSCs) monolayer derived from Porcine Wharton’s jelly. Porcine Wharton’s jelly MSCs (pWJ-MSCs) were
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The functional development of the rumen is influenced by weaning and associated with ruminal microbiota in lambs. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-02 Chong Li,Qian Zhang,Guoxiu Wang,Xiaolin Niu,Weimin Wang,Fei Li,Fadi Li,Zhaocai Zhang
Rumen development is critical for the development of early lambs. This work aims to evaluate the effects of abrupt weaning at day 21 on rumen fermentation, histomorphological traits and the ruminal microbiota compared with continuous suckling. Twelve pairs of artificially reared full-sib neonatal male Hu lambs were allocated to two groups, one of which was weaned at day 21 (EW group) and the other
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Specific phosphodiesterase type-10 inhibitor, papaverine, added after the cooling period improves canine sperm quality. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-02 Victoria Luño,Noelia González,Felisa Martínez,Beatriz González,Maite Olaciregui,Lydia Gil
The use of chilled semen has gained increasing interest in canine reproductive services. The addition of phosphodiesterase (PDE) inhibitors that increase the intracellular cyclic adenosine monophosphate levels may improve sperm motility. The purpose of this study was to examine the quality of sperm under the effect of the specific PDE-10 inhibitor (papaverine) added after storage for 1, 2, and 3 days
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Association of LEPR polymorphisms with egg production and growth performance in female Japanese quails. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-08-31 Mahmoud S El-Tarabany,Ayman A Saleh,Iman E El-Araby,Mohammed A El-Magd
This study aimed to screen intron 8 of the leptin receptor (LEPR) gene for polymorphisms in female Japanese quails. Two adjacent novel SNPs (A277G and A304G) were detected using PCR-SSCP and sequencing. These SNPs produced three haplotypes (AA/AA, AG/AG, and GG/GG) that were significantly (p ≤ 0.05) associated with growth and egg production traits. GG/GG haplotype-quails had significantly (p ≤ 0.05)
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Selection and validation of reference genes for RT-qPCR in adipose and longissimus dorsi muscle tissues of buffalo. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-08-31 Xue Feng,Xiaodan Cao,Ruirui Zhu,Jieping Huang
Real-time quantitative PCR (RT-qPCR) is widely used to measure and evaluate gene expression. The precision and reliability of RT-qPCR are critically dependent on the selection of suitable reference genes (RGs). In this study, an effort was made to identify the optimal RGs for RT-qPCR analysis of adipose and the longissimus dorsi muscle (LM) in buffaloes. RNA sequencing data were firstly analyzed to
更新日期:2020-08-31