-
Emergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-11-27 Marwan Osman,Iman Yassine,Monzer Hamze,Hiba Al Mir,Anahita Ghorbani Tajani,Bledar Bisha,Kevin J Cummings,Jean-Yves Madec,Marisa Haenni,Issmat I Kassem
Resistance to last resort antibiotics has been increasing, particularly in low- and middle-income countries such as Lebanon, which has well established challenges in antimicrobial stewardship and other public health and environmental issues. However, data on the emergence of antibiotic resistance in the community in Lebanon are limited. In this study, we assessed resistance to last resort antibiotics
-
Prevalence of Extended-Spectrum Beta-Lactamase Resistance and CTX-M-Group 1 Gene in Escherichia coli from the Water and Sediment of Urbanized Mangrove Ecosystems of Kerala. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-26 Gopika Sivan,Divya P Sukumaran,Akhil Prakash Ezhuthanikkunnel,Mohamed Hatha Ammanamveetil Abdulla
The study aimed to determine the prevalence of extended-spectrum β-lactamase resistance and CTX-M-group 1 gene in Escherichia coli from the water and sediment of three urbanized mangrove ecosystems of Kerala. A total of 119 E. coli isolates were screened for antibiotic susceptibility to 16 antibiotics. According to the phylogenetic analysis of E. coli isolates, nonpathogenic group A and pathogenic
-
Concomitant Resistance to Cefiderocol and Ceftazidime/Avibactam in Two Carbapenemase-Producing Klebsiella pneumoniae Isolates from Two Lung Transplant Patients. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-23 Greta Bellinzona,Cristina Merla,Marta Corbella,Elizabeth Nagy Iskandar,Elena Seminari,Angela Di Matteo,Stefano Gaiarsa,Greta Petazzoni,Davide Sassera,Fausto Baldanti,Aurora Piazza,Patrizia Cambieri
In this study, we present two cases of Klebsiella pneumoniae, one KPC-33- and one NDM-1-producing, showing resistance to cefiderocol and ceftazidime/avibactam, collected in the intensive care unit of a hospital in Northern Italy from two patients who had recently undergone lung transplantation. Whole-genome sequencing was performed to investigate the molecular features of these strains.
-
Aerococcus viridans and Public Health: Oral Carriage and Antimicrobial Resistance in Stray Dogs and Cats in Algeria. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-19 Kahina Razali,Luca Nalbone,Filippo Giarratana
The current study aimed to determine the occurrence and antimicrobial resistance of oral Aerococcus viridans in stray dogs and cats in Algeria. Oral swabs from 200 stray animals (100 dogs and 100 cats) were collected and cultured on Columbia agar medium supplemented with 5% defibrinated sheep blood. Isolates were identified using analytical profile index Rapid 20 Strep commercial kits, and antibiotic
-
Detection of Antimicrobial Resistance and Biofilm Production Among Staphylococcus pseudintermedius from Canine Skin Lesions. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-19 Sahar Nouri Gharajalar,Sadegh Tanhaee,Mahdieh Omidzadeh,Masoud Onsori
Aims: Staphylococcus pseudintermedius is an opportunistic pathogen also indicated as one of the major causes of skin infections in dogs. This study aimed to identify S. pseudintermedius isolated from canine skin lesions, evaluate their antibiotic resistance profile and biofilm production ability. Methodology: Lesions from 50 rural dogs with different skin lesions were sampled after pyoderma diagnosis
-
Clonal Dissemination of Candida auris Clinical Isolates in Northern Italy, 2021. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-17 Stefano Amadesi,Marta Palombo,Federica Bovo,Andrea Liberatore,Elena Vecchi,Monica Cricca,Tiziana Lazzarotto,Simone Ambretti,Paolo Gaibani
Candida auris is a concerning pathogen in health care due to its ability to spread in medical settings. In this study, we characterized the genome of three C. auris clinical isolates collected in the Emilia-Romagna region of Northeastern Italy from January 2020 to May 2021. Whole-genome sequencing was performed using Illumina iSeq 100 and Oxford Nanopore MinION systems. Genomes were assembled with
-
The Impact of Substitutions at Positions 1 and 8 of Fluoroquinolones on the Activity Against Mutant DNA Gyrases of Salmonella Typhimurium. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-10-04 Pondpan Suwanthada,Siriporn Kongsoi,Nami Miura,Lawrence P Belotindos,Chayada Piantham,Jirachaya Toyting,Mwangala L Akapelwa,Ruttana Pachanon,Kentaro Koide,Hyun Kim,Jeewan Thapa,Chie Nakajima,Yasuhiko Suzuki
Although many drug-resistant nontyphoidal Salmonella (NTS) infections are reported globally, their treatment is challenging owing to the ineffectiveness of the currently available antimicrobial drugs against resistant bacteria. It is therefore essential to discover novel antimicrobial drugs for the management of these infections. In this study, we report high inhibitory activities of the novel fluoroquinolones
-
High Prevalence of Carbapenem-Resistant Enterobacterales Colonization Among Intensive Care Unit Patients in a Tertiary Hospital, China. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-21 Shan-Jian Chen,Wei-Qing Zhang,Yu-Lan Lin,Yong-Bin Zeng,Shou-Tao Chen,Shu Wu,Zhen Xun,Bin Yang
Intestinal colonization with carbapenem-resistant Enterobacterales (CRE) has been shown as a significant risk factor for subsequent CRE infections, especially in intensive care units (ICUs). The aim of this study was to determine the prevalence of intestinal CRE colonization among ICU patients in a Chinese tertiary hospital. Fecal sample screenings for CRE were performed on ICU patients weekly. An
-
Study on the Genome and Mechanism of Tigecycline Resistance of a Clinical Chryseobacterium indologenes Strain. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-21 Yi Luo,Min Chen,Yujie Jiang,Weiqi Wang,Heping Wang,Li Deng,Zuguo Zhao
Purpose: Chryseobacterium indologenes is a clinically relevant microorganism that has been on the rise, with multidrug-resistant (MDR) strains being reported. C. indologenes carrying tet(X2) has been demonstrated to be resistant to the antibiotic tigecycline, yet, sensitive to all other members of the tetracycline family. This inconsistency in resistance prompts an inquiry into the contribution of
-
First Report of Antibiotic Resistance Markers cfiA and nim Among Bacteroides fragilis Group Strains in Ecuadorian Patients. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-21 Gabriela Sevillano,Ariane Paz Y Miño,María Belén Solís,Juan Pablo Vaca,Camilo Zurita-Salinas,Jeannete Zurita
In recent years, increasing resistance of Bacteroides fragilis to several antibiotics has been reported in different countries. The aim of this study was to evaluate the antibiotic resistance profiles of Bacteroides spp. isolated from clinical samples by phenotypic and molecular methods. A total of 40 nonrepetitive isolates of the B. fragilis group were studied from 2018 to 2019. The species was identified
-
Performance of Carbapenemase Nordmann-Poirel, Modified Carbapenem Inactivation, and EDTA Carbapenem Inactivation Methods for Detecting Carbapenem-Resistant Klebsiella pneumoniae Isolates. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-20 Kamalakar Sarva,Rameshkumar Marimuthu Ragavan,Lakshmi Jyothi Tadi,Sundaramurthy Raja,Arunagirinathan Narasingam
Infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are a major threat to public health. Timely detection of CRKP will help treat patients with appropriate antibiotics. This study aimed to evaluate the performance of the carbapenemase Nordmann-Poirel (CarbaNP), modified carbapenem inactivation (mCIM), and EDTA carbapenem inactivation (eCIM) methods for the detection of CRKP. We compared
-
Azole-Containing Agar Plates and Antifungal Susceptibility Testing for the Detection of Azole-Resistant Aspergillus Species in Hospital Environmental Samples. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-15 Mona Ghazanfari,Mahdi Abastabar,Iman Haghani,Maryam Moazeni,Shakiba Hedayati,Sanaz Yaalimadad,Bahador Nikoueian Shirvan,Felix Bongomin,Mohammad T Hedayati
The indoor environment of hospitals should be considered as an important reservoir of azole resistant Aspergillus species. In this study, we evaluated azole-containing agar plates (ACAPs) and antifungal susceptibility testing (AFST) for the detection of azole-resistant Aspergillus species in hospital environmental samples. Between September 2021 and January 2022, environmental samples (108 instruments
-
High Frequency of Methicillin-Resistant and Multidrug-Resistant Strains of Staphylococcus aureus Colonizing Students in Okada, Edo State, Nigeria. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-15 Maureen Uchechukwu Okwu,Augustine Obhioze Akpoka,Olley Mitsan,Osazee Ekundayo Izevbuwa,Anita Osamede,Jan Tkadlec
Staphylococcus aureus is one of the most common bacterial pathogens, often asymptomatically colonizing healthy people, but capable of causing fatal disease. The ability to treat S. aureus infections is limited by the rapid spread of multidrug-resistant strains. This study aimed to determine the prevalence of S. aureus carriage among students from Okada, Edo State, Nigeria, to analyze the antibiotic
-
Colistin Resistance in Multidrug-Resistant Escherichia coli Isolated from Retail Broiler Meat in Bangladesh. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-09-12 Chandan Nath,Tridip Das,Md Sirazul Islam,F M Yasir Hasib,Shuvo Singha,Avijit Dutta,Himel Barua,Md Zohorul Islam
The emergence of colistin resistance in Escherichia coli is a global public health concern. Contaminated food can accelerate the spread of colistin-resistant E. coli to humans. This study aimed to detect and characterize colistin-resistant E. coli from broiler meat in Bangladesh. We analyzed 136 pooled broiler meat samples from 240 carcasses collected from 40 live bird markets in urban and rural areas
-
Biocides in the Hospital Environment: Application and Tolerance Development. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-08-29 Catarina Geraldes,Luís Tavares,Solange Gil,Manuela Oliveira
Hospital-acquired infections are a rising problem with consequences for patients, hospitals, and health care workers. Biocides can be employed to prevent these infections, contributing to eliminate or reduce microorganisms' concentrations at the hospital environment. These antimicrobials belong to several groups, each with distinct characteristics that need to be taken into account in their selection
-
In Vitro Activity of Cefiderocol Against Carbapenem-Resistant Enterobacterales and Pseudomonas aeruginosa. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-08-22 Lucia Malisova,Iveta Vrbova,Katarina Pomorska,Vladislav Jakubu,Helena Zemlickova
The objective of this study was to assess the susceptibility of cefiderocol against multidrug-resistant carbapenemase-producing and nonproducing bacteria. The panel comprised 182 isolates of the order Enterobacterales, and 40 strains of Pseudomonas aeruginosa. Antimicrobial susceptibility testing has been performed using broth microdilution method according to the European Committee on Antimicrobial
-
hptA Mutation May Mediate Fosfomycin Resistance in Methicillin-Resistant Staphylococcus aureus Clinical Isolates. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-08-21 Jue Wang,Xiaogang Xu,Xiaoyu Zhao,Su Xu,Minggui Wang
Fosfomycin can be used alone or in combination to treat methicillin-resistant Staphylococcus aureus (MRSA) infection. However, fosfomycin resistance has been observed in MRSA. In S. aureus, fosfomycin resistance is mediated by the fosfomycin-modifying enzyme FosB, or mutations in the target enzyme MurA. Mutations in the chromosomal glpT and uhpT genes, which encode fosfomycin transporters, also result
-
Prevalence and In Vivo Assessment of Virulence in Shiga Toxin-Producing Escherichia coli Clinical Isolates from Greater Cairo Area. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-08-10 Rana Elshimy,Hamdallah Zedan,Tarek H Elmorsy,Rania Abdelmonem Khattab
Background: Shiga toxin-producing Escherichia coli (STEC) has been identified as an important etiologic agent of human disease in Egypt. Aims: To investigate the occurrence and describe the characterization as well as prevalence of STEC in Greater Cairo hospitals as well as molecular characterization of virulence and resistance genes. Methods: Four hundred seventy E. coli clinical isolates were collected
-
An Enterococcus faecium Isolated from Bovine Feces in Italy Shares optrA- and poxtA-Carrying Plasmids with Enterococci from Switzerland. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-31 Marzia Cinthi,Sonia Nina Coccitto,Serena Simoni,Carla Vignaroli,Andrea Brenciani,Eleonora Giovanetti
To investigate the occurrence of oxazolidinone resistance genes, 18 florfenicol-resistant enterococci were isolated from 66 fecal samples collected from several cattle farms in central Italy. The PCR screening indicated that only a bovine florfenicol-resistant isolate, Enterococcus faecium 249031-C, was positive for the presence of optrA and poxtA genes. The strain was tested for its susceptibility
-
Whole Genome Sequencing of an Extensively Drug-Resistant Raoultella planticola Isolate Containing blaKPC-2, blaNDM-1, and blaCTX-M-15. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-24 Paulo Victor Batista Marini,Eliandro Reis Tavares,Cintia Werner Motter,Letícia Busato Migliorini,Romário Oliveira de Sales,Nayara Helisandra Fedrigo,Danielle Rosani Shinohara,Mariangela Hungria,Sueli Fumie Yamada-Ogatta,Maria Cristina Bronharo Tognim
Raoultella planticola harboring genes that confer resistance to antimicrobials, such as carbapenems, have been associated with severe infections in immunocompromised patients. In this study, we reported the first whole genome sequence of a Brazilian isolate of R. planticola and the genomic context of antibiotic resistance markers. By whole-genome sequencing (WGS) of a carbapenem-resistant R. planticola
-
Multiplex Hybrid Capture Improves the Deep Detection of Antimicrobial Resistance Genes from Wastewater Treatment Plant Effluents to Assess Environmental Issues. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-11 Tsuyoshi Sekizuka,Nobuyasu Yamaguchi,Hajime Kanamori,Makoto Kuroda
Metagenomic sequencing (mDNA-seq) is one of the best approaches to address antimicrobial resistance (AMR) issues and characterize AMR genes (ARGs) and their host bacteria (ARB); however, the sensitivity provided is insufficient for the overall detection in wastewater treatment plant (WWTP) effluents because the effluent is well treated. This study investigated the multiplex hybrid capture (xHYB) method
-
Klebsiella quasipneumoniae subsp. similipneumoniae ST1859 O5:KL35 from Soil: First Report of qnrE1 in the Environment. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-10 Ralf Lopes,João Pedro Rueda Furlan,Micaela Santana Ramos,Lucas David Rodrigues Dos Santos,Rafael da Silva Rosa,Eliana Guedes Stehling
A Klebsiella quasipneumoniae subsp. similipneumoniae strain, named S915, belonging to the ST1859 O5:KL35, and harboring the plasmid-mediated quinolone resistance qnrE1 gene, was isolated from a soil sample cultivated with lettuce in Brazil. The core genome multilocus sequence typing analysis revealed that S915 strain was most related to a clinical strain of Brazil. Comparative genomic analysis showed
-
Cellular Attributes of Candida albicans Biofilm-Associated in Resistance Against Multidrug and Host Immune System. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-10 Dushyant Kumar,Awanish Kumar
One of the ubiquitous hospital-acquired infections is associated with Candida albicans fungus. Usually, this commensal fungus causes no harm to its human host, as it lives mutually with mucosal/epithelial tissue surface cells. Nevertheless, due to the activity of various immune weakening factors, this commensal starts reinforcing its virulence attributes with filamentation/hyphal growth and building
-
In Vitro Activity of Cefiderocol, Cefepime-Zidebactam, and β-Lactam Combinations Versus Other Antibiotic Classes Against Various Sequence Types of Clinically Isolated Carbapenemase-Producing Klebsiella pneumoniae. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-03 Phadungkiat Khamnoi,Noppadon Jumroon,Jakkrit Khamphakul,Narong Chaihongsa,Pitak Santanirand
Aim: This study aimed to establish the in vitro efficacy and susceptibility profiles of new β-lactam antibiotics against clinically isolated carbapenemase-producing Klebsiella pneumoniae (CPKP) strains. Materials and Methods: A total of 117 nonduplicated CPKP isolates were tested against cefiderocol, cefepime-zidebactam, ceftazidime-avibactam, tigecycline, and other 20 antibiotics by broth microdilution
-
RFS Proudly Announces the 2022 Award Recipient for Microbial Drug Resistance. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-07-01 Sabine Schuster
-
Treatment of Colistin Dependence-Developing Acinetobacter baumannii with Antibiotic Combinations at Subinhibitory Concentrations. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-06-28 Jeongwoo Jo,Ji Young Lee,Hongbaek Cho,Kwan Soo Ko
Recent studies have revealed that colistin dependence frequently develops in colistin-susceptible Acinetobacter baumannii isolates. Despite resistance in parental strains, colistin-dependent mutants showed increased susceptibility to several antibiotics, which suggests the possibility of developing strategies to eliminate multidrug-resistant (MDR) A. baumannii. We investigated in vitro and in vivo
-
Emerging Role of Sphingolipids in Amphotericin B Drug Resistance. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-06-15 Kashish Madaan,Vinay Kumar Bari
Invasive fungal infections in humans are common in people with compromised immune systems and are difficult to treat, resulting in high mortality. Amphotericin B (AmB) is one of the main antifungal drugs available to treat these infections. AmB binds with plasma membrane ergosterol, causing leakage of cellular ions and promoting cell death. The increasing use of available antifungal drugs to combat
-
In Vitro Activity of Cefiderocol Against Meropenem-Nonsusceptible Gram-Negative Bacilli with Defined β-Lactamase Carriage: SIDERO-WT Surveillance Studies, 2014-2019. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-30 Mark G Wise,James A Karlowsky,Meredith A Hackel,Miki Takemura,Yoshinori Yamano,Roger Echols,Daniel F Sahm
We examined the in vitro susceptibility of meropenem-nonsusceptible Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii complex isolates from five consecutive annual SIDERO-WT surveillance studies (2014-2019) to cefiderocol and comparator agents in the context of their carbapenemase carriage. 1,003 Enterobacterales, 1,758 P. aeruginosa, and 2,809 A. baumannii complex isolates from
-
Genomic Analysis of a Linezolid-Resistant Staphylococcus capitis Causing Bacteremia: Report from a University Hospital in Central Italy. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-24 Lucia Brescini,Simona Fioriti,Sonia N Coccitto,Marzia Cinthi,Marina Mingoia,Oscar Cirioni,Andrea Giacometti,Eleonora Giovanetti,Gianluca Morroni,Andrea Brenciani
Although coagulase negative staphylococci are rarely associated with complicated diseases, in some cases they cause life-threatening infections. Here we described a clinical case of a bacteremia due to a methicillin- and linezolid-resistant Staphylococcus capitis in a patient previously treated with linezolid. Whole genome sequencing revealed the common mutation G2576T in all rDNA 23S alleles and several
-
Antimicrobial Susceptibility Distributions of Clinical Isolates of Nontuberculous Mycobacteria in Israel. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-23 Yizhak Hershko,Amos Adler
There is a scarcity of data regarding the antimicrobial susceptibility testing profiles of nontuberculous mycobacterial (NTM) in Israel and other Middle Eastern countries. We aimed to describe the antimicrobial susceptibility profiles of NTM in Israel. A total of 410 clinical isolates of NTM, identified to the species level using matrix-assisted laser desorption ionization-time of flight mass spectrometry
-
Tigecycline-Containing Regimens and Multi Drug-Resistant Acinetobacter baumannii: A Systematic Review and Meta-Analysis. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-16 Fatemeh Sodeifian,Moein Zangiabadian,Erfan Arabpour,Naghmeh Kian,Fartous Yazarlou,Mehdi Goudarzi,Rosella Centis,Zahra Sadat Seghatoleslami,Mahdis Chahar Kameh,Bardia Danaei,Hossein Goudarzi,Mohammad Javad Nasiri,Giovanni Sotgiu,Giovanni Battista Migliori
Introduction: The use of tigecycline (TG) for the treatment of Acinetobacter baumannii is controversial. In this systematic review and meta-analysis, we aimed to better explore the safety and efficacy of TG for the treatment of multi drug-resistant (MDR) Acinetobacter. Methods: We searched PubMed/MEDLINE, Scopus, Cochrane Central, and Web of Science to identify studies reporting the clinical and microbiological
-
Clinical and Epidemiological Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Infections in a Tertiary Hospital in China. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-15 Zhiwen Cui,Lirui Wang,Min Feng
Purpose: Infections caused by carbapenem-resistant Klebsiella pneumoniae (CR-KP) are an important public health problem. This study aimed to evaluate the clinical characteristics of patients with CR-KP. Methods: A retrospective cohort study was conducted of all patients with CR-KP infection. A total of 615 patients with CR-KP infection were identified and 135 patients who did not meet the eligibility
-
Epidemiology, Phenotypic and Genotypic Characterization of Carbapenem-Resistant Gram-Negative Bacteria from a Libyan Hospital. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-05 Khouloud Slimene,Allaaeddin El Salabi,Olfa Dziri,Najla Mathlouthi,Seydina M Diene,Elhussan Ahmed Mohamed,Jadalla M A Amhalhal,Mohammed O Aboalgasem,Jomaa F Alrjael,Jean-Marc Rolain,Chedly Chouchani
Antimicrobial resistance, particularly resistance to carbapenems, has become one of the major threats to public health. Seventy-two isolates were collected from patients and hospital environment of Ibn Sina Hospital, Sirte, Libya. Antibiotic susceptibility tests, using the disc diffusion method and E-Test strips, were performed to select carbapenem-resistant strains. The colistin (CT) resistance was
-
Phylogenomic Analysis of CTX-M-15-Positive Escherichia coli from Companion Animal Reveals Intercontinental Dissemination of ST90 Within a One Health Framework. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-05-04 Luciana Sartori,Fábio P Sellera,Bruna Fuga,Elder Sano,Daniel F M Monte,Brenda Cardoso,Lucas de Angelis Côrtes,Nilton Lincopan
The global dissemination of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli has been considered a critical issue within a One Health framework. The aim of this study was to perform a genomic investigation of an ESBL-producing E. coli strain belonging to the globally spread sequence type/clonal complex ST90/CC23, isolated from gastrointestinal tract of a dog, in Brazil. Besides CTX-M-15
-
Comprehensive Drug Resistance Characterization of Pulmonary Tuberculosis in Algeria: Insights on Mycobacterium tuberculosis Strains by Whole-Genome Sequencing. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-04-28 Dalila Benremila,Ferhat Djoudi,Alima Gharout-Sait,Slimane Kheloufi,Andrea Spitaleri,Simone Battaglia,Andrea Maurizio Cabibbe,Daniela Maria Cirillo
In this study, we aimed to characterize drug-resistant strains by whole-genome sequencing (WGS), to describe the spreading lineages and the history of transmission. Drug susceptibility testing was performed by 96-well broth microdilution plates. The genomic DNA was extracted and purified; libraries were prepared and run on the Illumina NextSeq500 System. Among 82 isolates, 21 tuberculosis (TB) isolates
-
Virulence Characteristics and Drug Resistance of the Prevalent Capsule Types in Acinetobacter baumannii. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-04-17 Jiao Chen,Guanghui Li,Fen Wan,Peng Liu,Fangling Du,Yanting Shao,Qi Zhang,Zhibin Cheng,Yang Liu
Acinetobacter baumannii is a highly antibiotic-resistant pathogen causing nosocomial severe life-threatening infections, especially in critically ill patients. Capsular polysaccharide is a major virulence factor of A. baumannii both in vitro and in vivo. In this study, 220 isolates were collected in the hospital. The prevalent capsular types of A. baumannii were determined using polymerase chain reaction
-
Prevalence of fosA3 Gene in Fosfomycin-Resistant Proteus mirabilis in the Autonomous Community of Madrid (Spain) and Characterization of Two Positive Strains. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-04-06 Cristina Loras,María Pérez-Vázquez,Andrea González Prieto,Jesús Oteo-Iglesias,Juan-Ignacio Alós
Plasmid-mediated resistance to fosfomycin has been seldom described in Proteus mirabilis. We report two strains harboring fosA3 gene. Whole-genome sequencing revealed a plasmid that encoded fosA3 gene flanked by two insertion sequence (IS)26 mobile elements. Both strains also produced the blaCTX-M-65 gene that was located in the same plasmid. The sequence detected was IS1182-blaCTX-M-65-orf1-orf2-
-
Molecular Epidemiology of Haemophilus influenzae Strains with Reduced Susceptibility and Genetic Profiles of Resistance in the Postvaccination Period. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-04-05 Ingrid Menezes Brasil,Claudia Ferreira de Andrade,Dominique Mendes de Oliveira,Nathalia G S Caldeira,Jaciara Rodrigues de Oliveira,Ana Paula A do Nascimento,Antonio Eugênio C C de Almeida,Ivano de Filippis
Haemophilus influenzae serotype b has been the main cause of invasive infections in children, during the prevaccination period. More than 20 years after the introduction of the conjugate vaccine against Hib, HiNT has emerged as the cause of localized infections in children and adults. The main objective of this work is to evaluate the susceptibility and resistance mechanisms of H. influenzae strains
-
Designing New Magic Bullets to Penetrate the Mycobacterial Shield: An Arduous Quest for Promising Therapeutic Candidates. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-04-04 Anwita Mishra,Arghya Das,Tuhina Banerjee
Mycobacterium spp. intimidated mankind since time immemorial. The triumph over this organism was anticipated with the introduction of potent antimicrobials in the mid-20th century. However, the emergence of drug resistance in mycobacteria, Mycobacterium tuberculosis, in particular, caused great concern for the treatment. With the enemy growing stronger, there is an immediate need to equip the therapeutic
-
Characterization of Antimicrobial Resistance Genes and Virulence Factors in the Genomes of Escherichia coli ST69 Isolates from Preweaned Dairy Calves and Their Phylogenetic Relationship with Poultry and Human Clinical Strains. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-24 Serajus Salaheen,Seon Woo Kim,Hayley R Springer,Ernest P Hovingh,Jo Ann S Van Kessel,Bradd J Haley
Escherichia coli sequence type 69 (ST69) are common causative agents of extraintestinal infections occurring in the bloodstream, cerebrospinal fluid, surgical sites, and, most frequently, the urinary tract. The objective of this study was to analyze the genomic characteristics of 45 antimicrobial-resistant Escherichia coli ST69 strains that were isolated from 28 calves on eight dairy farms in Pennsylvania
-
Evaluation of the MDR Direct Flow Chip Kit for the Detection of Multiple Antimicrobial Resistance Determinants. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-24 Ángel Rodríguez-Villodres,Antonio Galiana-Cabrera,Ignacio Torres Fink,Rosario Duran Jiménez,José Miguel Cisneros,José Antonio Lepe
The objective of this study was to evaluate the accuracy of the MDR Direct Flow Chip Kit for the detection of antimicrobial resistance (AMR) determinants from bacterial colonies. Ninety-two clinical isolates with known AMR determinants genotypically characterized were used. The MDR Direct Flow Chip Kit is a microarray-based assay that included 55 AMR determinants for beta-lactams (23), quinolones (13)
-
Clinical Management of a Pandrug-Resistant OXA-48 Klebsiella pneumoniae Infection in the Pediatric Intensive Care Unit. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-13 Merve Havan,Tanıl Kendirli,Özgün Tutku Parlar,Serhan Özcan,Aysun Yahşi,Tuğba Erat,Duygu Öcal,Haluk Guriz,Halil Özdemir,Zeynep Ceren Karahan,Ergin Çiftci,Erdal İnce
Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is one of the serious forms of health care-associated infection. Pan-drug resistant (PDR) CRKP infections can cause severe infections. Mortality and treatment costs in the pediatric intensive care unit (PICU) are high. This study aims to share our experience regarding the treatment of oxacillinase (OXA)-48-positive PDR-CRKP infection in
-
Outer Membrane Vesicles Associated with the Delivery of Amoxicillin Resistance in Glaesserella parasuis. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-10 Jiahui An,Chao Zhang,Yufeng Li
Glässer's disease is caused by Glaesserella parasuis, a common bacterium in the upper respiratory tract of pigs. Antibiotics are widely used to control this disease. A G. parasuis isolate with amoxicillin (AMX) resistance was identified in our previous study. Outer membrane vesicles (OMVs) are naturally released by G. parasuis and contain many compounds. To reveal the underlying mechanisms associated
-
Induction of Antimicrobial Resistance of Stenotrophomonas maltophilia by Exposure to Nonlethal Levels of Antibiotics. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-10 Yaiprae Chatree,Nisanart Charoenlap,Veerakit Vanitshavit,Piyatida Ruangrassamee,Skorn Mongkolsuk,Paiboon Vattanaviboon
The effects of the sequential subculture in the presence of a driving force on antimicrobial resistance of Stenotrophomonas maltophilia K279a were investigated. Stationary-phase cells were inoculated into the lysogeny broth medium, with and without antibiotic supplementation, and grown until the stationary phase before being subcultured into the same antibiotic-supplemented medium for six consecutive
-
Development of a Real-Time PCR Assay for Detection of Macrolide Resistance Mutations in Mycoplasma genitalium and Its Application for Epidemiological Surveillance in Russia. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-01 Inna Alexandrovna Edelstein,Andrey Vyacheslavovich Romanov,Roman Sergeevich Kozlov
A real-time PCR assay based on probe fluorescence quenching by a primer was developed and validated for detection of macrolide resistance (MR)-associated mutations in the 23S rRNA gene of Mycoplasma genitalium. The assay involves identification of any nucleotide substitutions at positions 2058, 2059, and 2611 of 23S rRNA (Escherichia coli numbering) by the probe-based melting curve analysis immediately
-
Plasmid-Mediated Colistin Resistance Genes mcr-1 and mcr-4 in Multidrug-Resistant Escherichia coli Strains Isolated from a Healthy Pig in Portugal. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-03-01 Ana Amaro,Célia Leão,Vanessa Guerra,Teresa Albuquerque,Lurdes Clemente
Antimicrobial resistance encoded by mobile colistin resistance (mcr) genes is a global and emergent threat. In this study, we report the occurrence of two different populations of colistin-resistant Escherichia coli harboring mcr-1 and mcr-4 variants in the intestinal microbiome of a healthy pig. Following antimicrobial susceptibility determination, the presence of mcr genes in two E. coli strains
-
How Does Time Affect the Antimicrobial Activity of Super-Oxidized Commercial Antiseptic Solutions? An In Vitro Test. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-28 Marco S Perez-Ayala,José Antonio Alvarez,Alejandro E Macias,Brenda J Torres-Murillo
This study aimed to identify variation in the minimum biocidal concentration (MBC) over time, comparing three commercial super-oxidized solutions with different chemical compositions. In the bactericidal assay, the following bacteria were tested: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and for each ATCC, one wild-type strain was used.
-
Re: "Molecular Characterization of Carbapenem-Resistant Enterobacterales Collected in the United States" by Karlsson et al. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-27 Helio S Sader,Rodrigo E Mendes,Mariana Castanheira
-
Treatment Failure Due to Adaptive Resistance Mechanisms in a Severe and Complicated Bloodstream Infection Due to Elizabethkingia meningoseptica. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-24 Adam G Stewart,Jason A Roberts,Brian M Forde,Haakon Bergh,Timothy J Kidd,Hugh Wright,Patrick N A Harris
Elizabethkingia meningoseptica is an uncommonly encountered multidrug-resistant gram-negative bacterium that causes infections primarily among vulnerable hosts. A true opportunistic pathogen, its ability to cause severe sepsis and complicated infection in selected patients has been noted. Very limited preclinical and clinical data exist with regard to suitable therapeutic options. In this study, we
-
Antimicrobial Resistance Among Gram-Negative Bacteria Isolated in the Newborn Intensive Care Unit at Abderrezak-Bouhara Hospital of Skikda, Algeria. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-22 Asma Labid,Sarah Benouagueni,Aida Mehainaoui,Djamila Gacemi Kirane,Abdelaziz Touati
Background: This study aimed to determine the epidemiology of gram-negative bacteria (GNB) isolated in the newborn intensive care unit (NICU) population, to assess their antibiotic susceptibility patterns and possible associated risk factors. Methods: All neonates admitted to the NICU of Abderrezak-Bouhara hospital (Skikda, Algeria) with a clinical diagnosis of neonatal infections from March to May
-
Molecular Mechanisms of MmpL3 Function and Inhibition. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-21 John T Williams,Robert B Abramovitch
Mycobacteria species include a large number of pathogenic organisms such as Mycobacterium tuberculosis, Mycobacterium leprae, and various non-tuberculous mycobacteria. Mycobacterial membrane protein large 3 (MmpL3) is an essential mycolic acid and lipid transporter required for growth and cell viability. In the last decade, numerous studies have characterized MmpL3 with respect to protein function
-
Seven Years of Culture Collection of Neisseria gonorrhoeae: Antimicrobial Resistance and Molecular Epidemiology. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-08 Anna Carannante,Paola Vacca,Stefano Fontana,Ivano Dal Conte,Valeria Ghisetti,Marco Cusini,Grazia Prignano,Caterina Vocale,Anna Maria Barbui,Elena Stroppiana,Marina Busetti,Antonella Mencacci,Marina Rotondi,Maria Antonia De Francesco,Carmen Luciana Bonanno,Patrizia Innocenti,Maria Agnese Latino,Eleonora Riccobono,Federica Poletti,Ines Clotilde Casonato,Graziella Soldato,Luigina Ambrosio,Stefano Boros
The emergence of Neisseria gonorrhoeae isolates displaying resistance to antimicrobials, in particular to ceftriaxone monotherapy or ceftriaxone plus azithromycin, represents a global public health concern. This study aimed to analyze the trend of antimicrobial resistance in a 7-year isolate collection retrospective analysis in Italy. Molecular typing on a subsample of gonococci was also included.
-
Antibiotic Resistance of Helicobacter pylori in Turkey: A Systematic Review and Meta-Analysis Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-01 Meltem Ayaş, Yeşim Gürol
Turkey presents both a high prevalence of Helicobacter pylori infection and high prevalence of antibiotic resistance. In this study, we aimed to summarize recent data on H. pylori antibiotic resistance rates in this nation. After conducting searches in two national and international databases (ULAKBIM, EKUAL, and PubMed), a systematic review was conducted. A total of 197 original articles on antibiotic
-
Genetic and Phenotypic Characterization of the First Canadian Case of Ambler Class A Carbapenemase FRI-8. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-01 Laura F Mataseje,Florence Doualla-Bell,David A Boyd,Ken Fakharuddin,Hector Felipe Garcia Jeldes,Valerie Plante,Marie-Claude Beaudoin,Alexandre Godbout,Simon Wong,Judith Fafard,Michael R Mulvey
This study investigated the mechanism of carbapenem resistance in an Enterobacter cloacae complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of Enterobacter
-
Letter to the Editor: Novel Antimicrobial Genetic Elements in Methicillin-Resistant Macrococcus armenti. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-01 Jennifer Eleonora Keller,Sybille Schwendener,Dana Nováková,Roman Pantůček,Vincent Perreten
-
Mutations in Genes Encoding 23S rRNA and FadD32 May be Associated with Linezolid Resistance in Mycobacteroides abscessus. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-01 Hien Fuh Ng,Yun Fong Ngeow
Linezolid is one of the antibiotics used to treat the Mycobacteroides abscessus infection. However, linezolid-resistance mechanisms of this organism are not well understood. The objective of this study was to identify possible linezolid-resistance determinants in M. abscessus through characterization of step-wise mutants selected from a linezolid-susceptible strain, M61 (minimum inhibitory concentration
-
Evaluation of Early Reading of Broth Microdilution Technique for Polymyxin B. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-02-01 Maiara Dos Santos Carneiro,Fabiana Caroline Zempulski Volpato,Camila Mörschbächer Wilhelm,Priscila Lamb Wink,Afonso Luís Barth
Delay in the results of standard phenotypic susceptibility tests is the main obstacle to adequate antibiotic treatment. For this reason, the European Committee for Antimicrobial Susceptibility Testing has proposed the Rapid Antimicrobial Susceptibility Testing for the disk diffusion method directly from blood culture. However, to date, there are no studies evaluating early readings of polymyxin B broth
-
First Report of OXA-48 and IMP Genes Among Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates from Diarrheic Calves in Tunisia Microb. Drug Resist. (IF 2.6) Pub Date : 2023-01-24 Asma Ben Haj Yahia, Ghassan Tayh, Sarrah Landolsi, Elaa Maamar, Nejia Galai, Zbaida Landoulsi, Lilia Messadi
Antimicrobial resistance is one of the most serious threats to human and animal health. Evidence suggests that the overuse of antimicrobial agents in animal production has led to the emergence and dissemination of multidrug-resistant isolates. The objective of this study was to assess the rate of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in calf feces and to characterize their
-
Letter to the Editor: A Porcine Enterococcus hirae Shares a poxtA-Carrying Plasmid with a Human Enterococcus faecium Isolate. Microb. Drug Resist. (IF 2.6) Pub Date : 2023-01-25 Marzia Cinthi,Sonia Nina Coccitto,Serena Simoni,Cristiana Garofalo,Cristiana Cesaro,Carla Vignaroli,Andrea Brenciani,Eleonora Giovanetti
-
WHO-Point Prevalence Survey of Antibiotic Use Among Inpatients at a Core National Antimicrobial Consumption Network Site in North India: Findings and Implications Microb. Drug Resist. (IF 2.6) Pub Date : 2023-01-17 Niti Mittal, Rakesh Mittal, Nidhi Goel, Aparna Parmar, Arti Bahl, Suneet Kaur, Kavita R. Gudibanda, Vibhor Dudhraj, Sujeet K. Singh
Data on Point Prevalence Surveys (PPSs) in India are limited yet. We report findings of a PPS conducted in a core “National Antimicrobial Consumption Network site” under National Centre for Disease Control - WHO project “Point prevalence survey of antimicrobial consumption at healthcare facilities.” A cross-sectional survey was conducted as per the “WHO methodology for PPS on antibiotic use in hospitals”