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Characterization of Third-Generation Cephalosporin-Resistant Escherichia coli Isolated from Pigs in Cuba Using Next-Generation Sequencing Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-19 Rosa Elena Hernández-Fillor; Michael Brilhante; Carelia Martha Marrero-Moreno; Michel Baez; Ivette Espinosa; Vincent Perreten
Resistance to third-generation cephalosporins (3GC) in Escherichia coli has been reported worldwide from humans and animals, but the situation in Cuba is still poorly understood. This study aimed to gain new insights into the phenotypic and genotypic characteristics of third-generation cephalosporin-resistant (3GC-R) E. coli isolated from pigs in Cuba. Rectal swabs from 215 healthy pigs were taken
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Antibiotic Tolerance in Biofilm and Stationary-Phase Planktonic Cells of Staphylococcus aureus. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Ekta Kamble,Karishma Pardesi
The ability of Staphylococcus aureus to form biofilms and persisters is a major cause of recalcitrant infections that are difficult to treat. We have examined time-dependent variation in persister population present in stationary-phase planktonic cells and biofilms of S. aureus when treated with bactericidal antibiotics having different cellular targets. Fourteen isolates identified as S. aureus were
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Methylsulfonylmethane Exhibits Bacteriostatic Inhibition of Vancomycin-Resistant Enterococcus faecium, In Vitro. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Toni L Poole,Rodney Benjamin,Kenneth J Genovese,David J Nisbet
The aim of this study was to evaluate the antibacterial properties of methylsulfonylmethane (MSM) on vancomycin-resistant Enterococcus faecium (VRE). Bacterial proliferation was measured spectrophotometrically during growth in brain heart infusion broth with 0%, 3%, 5%, 7%, 10%, 12%, and 16% MSM. To assess the mechanism of inhibition, VRE was grown overnight with 0–16% MSM and enumerated on unmedicated
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Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase, Plasmid-Mediated Quinolone Resistance, and Carbapenemase-Producing Gram-Negative Bacilli in Burkina Faso. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Soufiane Sanou,Abdoul Salam Ouedraogo,Salim Aberkane,Julie Vendrell,Oumar Ouchar,Nicolas Bouzimbi,Arsène Hema,Armel Poda,Jacques Zoungrana,Georges A Ouedraogo,Christian Carrière,Hélène Jean-Pierre,Rasmata Ouedraogo/Traore,Sylvain Godreuil
The spreading of carbapenemase-producing gram-negative bacilli (GNB) must be considered as an “urgent” threat. The aim of this study was to determine the prevalence of extended spectrum β-lactamase (ESBL), plasmid-mediated quinolone resistance (PMQR), and carbapenemase-producing GNB and to characterize the supporting genes in GNB specimens isolated from patients and healthy volunteers in Burkina Faso
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Environmental Phage-Based Cocktail and Antibiotic Combination Effects on Acinetobacter baumannii Biofilm in a Human Urine Model. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Bartłomiej Grygorcewicz,Bartosz Wojciuk,Marta Roszak,Natalia Łubowska,Piotr Błażejczak,Joanna Jursa-Kulesza,Rafał Rakoczy,Helena Masiuk,Barbara Dołęgowska
The emergence of multidrug-resistant (MDR) bacterial infections poses a catastrophic threat to medicine. The development of phage-based therapy combined with antibiotics might be an advantageous weapon in the arms race between human and MDR bacteria. A cocktail composed of the MDR Acinetobacter baumannii infecting bacteriophages with high lytic activity was used in combination with antibiotics to destroy
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Prevalence of Extended-Spectrum Beta-Lactamase/Carbapenemase Genes and Quinolone-Resistance Determinants in Klebsiella pneumoniae Clinical Isolates from Respiratory Infections in Myanmar. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Meiji Soe Aung,Nyein Chan Win,Nilar San,Myat Su Hlaing,Yi Yi Myint,Pyae Phyo Thu,Myint Thazin Aung,Kyaw Thu Yaa,Win Win Maw,Noriko Urushibara,Nobumichi Kobayashi
In recent years, nosocomial infections due to multidrug resistant Klebsiella pneumoniae strains have been increasing, associated with growing trend of resistance to beta-lactams and fluoroquinolones (FQs) worldwide. In this study, prevalence of beta-lactamase genes and resistance mechanisms to FQ were analyzed in 191 clinical K. pneumoniae isolates derived from respiratory tract infections in a teaching
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High Prevalence of 16s rRNA Methylase Genes Among Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae Isolates in a Chinese Tertiary Hospital. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Wenjian Liao,Liang De Wang,Dan Li,Fang-Ling Du,Dan Long,Yang Liu,OonTek Ng,Wei Zhang
Thirty-nine carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates collected from a Chinese tertiary hospital were used in the characterization of the prevalence of 16S rRNA methylase genes. In total, 66.7% (26/39) of the CR-hvKP isolates were found to carry 16S rRNA methylase genes. The most frequently detected 16S rRNA methylase gene was armA (11/26, 42.3%), followed by rmtB
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Prevalence, Epidemiology, Etiology, and Sensitivity of Invasive Bacterial Infections in Pediatric Patients Undergoing Oncological Treatment: A Multicenter Nationwide Study. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Olga Zajac-Spychala,Jacek Wachowiak,Olga Gryniewicz-Kwiatkowska,Aneta Gietka,Bozenna Dembowska-Baginska,Katarzyna Semczuk,Katarzyna Dzierzanowska-Fangrat,Krzysztof Czyzewski,Magdalena Dziedzic,Mariusz Wysocki,Patrycja Zalas-Wiecek,Anna Szmydki-Baran,Lukasz Hutnik,Michal Matysiak,Filip Pierlejewski,Wojciech Mlynarski,Zofia Małas,Wanda Badowska,Ninela Irga-Jaworska,Ewa Bien,Elzbieta Drozynska,Magdalena
Background: Infectious complications (IC) caused by bacterial strains often impede anticancer therapy. The study aimed to retrospectively analyze bacterial IC that could help predict the risk and optimize the empirical treatment for bacterial infections in pediatric cancer patients.
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Antimicrobial Resistance of Serial Isolates of Acinetobacter baumannii Colonizing the Rectum of Adult Intensive Care Unit Patients in a Teaching Hospital in Kuwait. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Ghayda Al-Hashem,Vincent O Rotimi,M John Albert
Objectives: Outbreak and endemic isolates of Acinetobacter baumannii are known to be polyclonal. In an ongoing study, we hypothesized that the patient gut was the source of the polyclonality where genetic exchanges take place. To test the hypothesis, we collected 270 serial rectal isolates from 32 adult intensive care unit patients over 16 months and investigated their drug resistance profiles.
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Phenotypic and Genotypic Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli: A Systematic Review and Meta-analysis in Iran. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Leila Jabalameli,Reza Beigverdi,Hamidreza Hagh Ranjbar,Ramin Pouriran,Fereshteh Jabalameli,Mohammad Emaneini
Background: Despite the existence of discrete and varied studies regarding extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) in Iran, a comprehensive analysis on the prevalence of ESBL-EC has not yet been carried out. The current study analyzed published data regarding ESBL-EC in different regions of Iran to gain insight into this significant subject.
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Characterization of Multidrug-Resistant Isolates of Salmonella enterica Serovars Heidelberg and Minnesota from Fresh Poultry Meat Imported to Portugal. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Leonor Silveira,Alexandra Nunes,Ângela Pista,Joana Isidro,Cristina Belo Correia,Margarida Saraiva,Rita Batista,Isabel Castanheira,Jorge Machado,João Paulo Gomes
Salmonella enterica serovars Heidelberg and Minnesota frequently display several genetic mobile elements making them potential spreaders of resistance genes. Here, we phenotypically determined the antibiotic resistance profile and subsequently performed whole-genome sequencing on 36 isolates recovered from samples of fresh poultry meat, within the Portuguese Official Inspection Plan for Imported Foodstuffs
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Occurrence, Antimicrobial Resistance Patterns, and Genetic Characterization of Staphylococcus aureus Isolated from Raw Milk in the Dairy Farms over Two Seasons in China. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Cuiping Shi,Zhongna Yu,Harvey Ho,Jun Wang,Wei Wu,Mengru Xing,Yutao Wang,S M E Rahman,Rongwei Han
This study investigated the occurrence and resistance rates of Staphylococcus aureus strains isolated from raw milk in the dairy farms over two seasons (spring and autumn) and across four regions that included 11 provinces in China. In total, 750 raw milk samples from the 405 dairy farms were collected. Fifteen antimicrobial agents were tested for antimicrobial resistance via disk diffusion tests,
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Phylogenetic Characterization and Multilocus Sequence Typing of Extended-Spectrum Beta Lactamase-Producing Escherichia coli from Food-Producing Animals, Beef, and Humans in Southwest Nigeria. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Olusolabomi J Adefioye,Jörg Weinreich,Stefan Rödiger,Peter Schierack,Olugbenga Adekunle Olowe
Multidrug-resistant extended-spectrum beta lactamase (ESBL)-producing Escherichia coli strains are emerging globally in both humans and animals. Antimicrobial susceptibility testing and ESBL screening were performed on pure cultures of 216 E. coli isolates from human and animal fecal samples as well as beef. Polymerase chain reaction was performed for the detection of resistance genes. Representative
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Plasmid-Determined Colistin Resistance in the North African Countries: A Systematic Review. Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-15 Abdelaziz Touati,Assia Mairi
We have conducted a systematic review to update available information on plasmid-mediated colistin resistance (mobilized colistin resistance [mcr]) genes in North African countries. We have searched the articles of PubMed, Scopus, and Web of Science databases reporting plasmid-mediated colistin resistance bacteria isolated in North African countries. After searching and selection, 30 studies that included
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Antimicrobial and Antibiofilm Activities and Mechanism of Action of Chelerythrine Against Carbapenem-Resistant Serratia marcescens In Vitro Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-13 Wei-Dong Qian; Jie Huang; Jia-Ning Zhang; Xin-Cheng Li; Yi Kong; Ting Wang; Yong-Dong Li
Aim: To evaluate the antimicrobial and antibiofilm effects of chelerythrine (CHE) against carbapenem-resistant Serratia marcescens (CRSM).
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Colistin-Resistant Enterobacterales Isolated from Chicken Meat in Western Algeria Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-11 Nadia Chaalal; Abdelaziz Touati; Alex Yahiaoui-Martinez; Mohamed Amine Aissa; Albert Sotto; Jean-Philippe Lavigne; Alix Pantel
Aim: In Algeria, colistin is used as a metaphylactic treatment in the poultry industry for the treatment of Gram-negative gastrointestinal infections and also as a feed additive to promote animal growth. The aim of this study was to investigate the importance and genetic characteristics of colistin-resistant Enterobacterales from chicken meat in Western Algeria.
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Carbapenem–Resistant Gram Negative Bacilli Are Predominantly Multidrug or Pan-Drug Resistant Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-08 Priya Kannian; Pasuvaraj Mahanathi; Veeraraghavan Ashwini; Muthu Vaishnavi; Chandran Priya
Carbapenems, although originally introduced against multidrug–resistant (MDR) Gram negative bacilli (GNB), are now advocated for initial empiric use resulting in increasing carbapenem-resistant (CR) GNB. In this study, we analyzed the frequencies of CR-GNB and compared their resistance patterns against other antibiotics. Overall, 42% (1,014/2,420) of CR-GNB were isolated (range: 29–59%), with similar
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Fitness Cost of Antibiotic Resistance in Staphylococcus aureus: A Systematic Review Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-08 Abdelaziz Touati; Zahra Bellil; Damia Barache; Assia Mairi
Background: Recent reports have shown the potential of Staphylococcus aureus for acquiring resistance to last-resort antibiotics. However, most antibiotic resistance mechanisms were associated with a fitness cost that was typically observed as a reduced bacterial growth rate. This systematic review aimed to address the fitness cost of antibiotic resistance in S. aureus that emerged by mutations.
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Epidemiological and Genetic Features of Plasmids Carrying blaNDM Genes: An In Silico Analysis with Emphasis on Replicon Types, and Resistome Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-08 Abdelaziz Touati; Lyticia Manseur; Imene Mehidi; Assia Mairi
Background: New Delhi metallo-β-lactamase (NDM) is a metallo-β-lactamase that has been disseminated worldwide. Plasmids harboring the blaNDM gene belonged to many incompatibility groups, of which IncX3, IncF, and IncA/C were the most represented. This in silico study aimed at analyzing a set of 649 plasmids carrying NDM-type carbapenemase (pNDMs) previously assigned in GenBank.
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Evaluation of a Phenotypic Algorithm to Direct Carbapenemase Testing in Pseudomonas aeruginosa: Validation in a Multicenter German Cohort Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-08 Christian M. Gill; Michael Kresken; Harald Seifert; David P. Nicolau
Pseudomonas aeruginosa remains a prominent nosocomial pathogen. Detection of carbapenemase-producing P. aeruginosa is vital to dictate antimicrobial therapy and infection control measures. A pragmatic, minimum inhibitory concentration-based algorithm using imipenem AND meropenem-resistant plus ceftazidime-, cefepime-, and piperacillin/tazobactam-nonsusceptible criterion was derived to guide carbapenemase
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Potentially Pathogenic Multidrug-Resistant Escherichia coli in Lamb Meat Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-08 Katia Suemi Gozi; Luana Perpetua Tobias Deus Ajude; Marlon do Valle Barroso; Caroline Rodrigues da Silva; Juliana Regina Peiró; Luiz Claudio Nogueira Mendes; Mara Corrêa Lelles Nogueira; Tiago Casella
Extended-spectrum cephalosporin (ESC) resistance remains a threat since ESC are important antimicrobials used to treat infections in humans and animals. Escherichia coli is an important source of ESC-resistance genes, such as those encoding extended-spectrum β-lactamases (ESBLs). E. coli is a common commensal of lambs. Reports that contaminated food can be a source of ESC-resistant bacteria in humans
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Comparison of Conventional Methods, Automated Systems, and DNA Sequence Analysis Methods in the Identification of Corynebacterium afermentans and Corynebacterium mucifaciens Bacteria Isolated from Blood and Catheter Culture Samples Microb. Drug Resist. (IF 2.296) Pub Date : 2021-01-07 Serpil Ölmez; Özlem Tuncer; Mehmet Parlak; Asiye Bıçakçıgil; Nafia Canan Gürsoy; Barış Otlu; Hüseyin Güdücüoğlu; Banu Sancak
The aim of this study is to compare different methods due to the difficulties in identifying coryneform bacteria to species level and to determine antibiotic resistance profiles. Isolates identified as Turicella otitidis (n:45) by VITEK 2 Compact and Corynebacterium mucifaciens (n:1) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), isolated from blood
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Exploring the Prevalence and Distribution Patterns of Antibiotic Resistance Genes in Bovine Gut Microbiota Using a Metagenomic Approach Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-31 Zhen Zhu; Mingze Cao; Weiwei Wang; Liwei Zhang; Tenghe Ma; Guanhui Liu; Yue Zhang; Zixuang Shang; Xu Chen; Yuxiang Shi; Jiyu Zhang
Antibiotic resistance genes (ARGs) have become recognized contaminants and pose a high public health risk. The animal gut microbiota is a reservoir of ARGs, but the knowledge of the origin and dissemination of ARGs remains unclear. In this study, we provide a comprehensive profile of ARGs and mobile genetic elements in the gut microbiota from 30 bovines to study the impact of modern antibiotics on
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Genomic and Phenotypic Analysis of Persistent Carbapenem-Resistant Klebsiella pneumoniae Isolates from a 5-Year Hospitalized Patient Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-23 Zhiqiang Yang; Qiaoling Sun; Shang Chen; Shuangyang Ding; Rong Zhang; Kui Zhu
The emerging epidemic of carbapenem-resistant Klebsiella pneumoniae (CRKP) is a global public health crisis. However, the phylogenetic affiliation and pathotypic status of CRKP strains in the host colonization period under consistent antibiotic treatments are not well characterized. In this study, a 5-year tracking study was performed, in which a patient admitted to an intensive care unit was recruited
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What Is Driving the Epidemiology of Methicillin-Resistant Staphylococcus aureus Infections in Europe? Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-18 Michael A. Borg; Liberato Camilleri
Introduction: The burden from infections caused by methicillin-resistant Staphylococcus aureus (MRSA) in the European Union/European Economic Area (EU/EEA) has increased in recent years, especially in the higher prevalence southern and eastern countries. Addressing this challenge requires a clear knowledge of the factors driving this epidemiology to inform effective national interventions and campaigns
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Molecular Characterization of the First Emerged NDM-1-Producing Pseudomonas aeruginosa Isolates in South Korea Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-17 Jun Sung Hong; Wonkeun Song; Min-Jeong Park; Seri Jeong; Nuri Lee; Seok Hoon Jeong
Carbapenemase-producing Pseudomonas aeruginosa (CPPA) is a threat to public health. This study aimed to describe the first emergence and molecular characterization of NDM-1-producing P. aeruginosa in South Korea. A total of 183 carbapenem-resistant P. aeruginosa (CRPA) isolates were recovered from patients at a university hospital in Seoul, South Korea. The antimicrobial resistance genes and their
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The Antibacterial Agent Identified from Acidocella spp. in the Fluid of Nepenthes gracilis Against Multidrug-Resistant Klebsiella pneumoniae: A Functional Metagenomic Approach Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-16 Elaine Wan Ling Chan; Mei Yu Chin; Yi Hui Low; Hui Yin Tan; Yi Sing Ooi; Chun Wie Chong
Aims: The fluid of Nepenthes gracilis harbors diverse bacterial taxa that could serve as a gene pool for the discovery of the new genre of antimicrobial agents against multidrug-resistant Klebsiella pneumoniae. The aim of this study was to explore the presence of antibacterial genes in the fluids of N. gracilis growing in the wild.
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Distribution and Molecular Characterization of Escherichia coli Harboring mcr Genes Isolated from Slaughtered Pigs in Thailand Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-15 Aunyarat Khanawapee; Anusak Kerdsin; Peechanika Chopjitt; Parichart Boueroy; Rujirat Hatrongjit; Yukihiro Akeda; Kazunori Tomono; Suphachai Nuanualsuwan; Shigeyuki Hamada
The resistance of Enterobacteriaceae to colistin mediated by plasmid-borne mobile mcr genes is an emerging public health concern. This study aimed to explore the distribution and characteristics of Escherichia coli isolates harboring mcr genes from slaughtered pigs in Thailand from 2014 to 2015. A total of 779 E. coli isolates were assessed, of which 61 (7.8%) were found to carry mcr genes, including
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Genotyping and Molecular Characterization of Fluoroquinolone's Resistance Among Multidrug-Resistant Mycobacterium tuberculosis in Southwest of China Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-10 Yan Hu; Jie Liu; Jing Shen; Xin Feng; Wenguo Liu; Damian Zhu; Huiwen Zheng; Daiyu Hu
Although fluoroquinolones (FQs) are the backbone drugs for the treatment of multidrug-resistant tuberculosis (MDR-TB), the knowledge about the resistance pattern and molecular characterization of new-generation FQs in Chongqing is limited. This study aimed to investigate the resistance rate and mutation types of later-generation FQs against MDR-TB in Chongqing, and further to explore the relationship
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Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-10 Anthony Demord; Laurent Poirel; Fernando D'Emidio; Stefano Pomponio; Patrice Nordmann
Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.
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Antibiogram, Virulence Genes, and Biofilm-Forming Ability of Clinical Salmonella enterica Serovars: An In Vitro Study Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-10 Dhiraj Krishna; Biranthabail Dhanashree
Salmonella enterica serovar Typhi and Salmonella Paratyphi are causative agents of enteric fever. Salmonella Typhi persists as a biofilm on gallstones. Hence, we studied the biofilm formation, antibiogram, and virulence genes of S. enterica serovars. Antibiogram of S. enterica serovars from human blood and stool samples were studied by Kirby–Bauer disk diffusion method and biofilm by microtiter plate
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In Vivo High Plasticity of Multi-Drug Resistant ST258 Klebsiella pneumoniae Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-04 Virginia Garcia-Fulgueiras; Carmen Magallanes; Victoria Reyes; Camila Cayota; Antonio Galiana; Mariela Vieytes; Rafael Vignoli; Carolina Márquez
Carbapenemase production in Enterobacterales clinical isolates is a global threat. Multi-drug resistant Klebsiella pneumoniae harboring carbapenemases are a major concern among the hospital settings in Latin America.
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Antibiofilm Potential of Arenecarbaldehyde 2-Pyridinylhydrazone Derivatives Against Acinetobacter baumannii. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Priscila Cristina Bartolomeu Halicki,Vanessa Radin,Andrea von Groll,Marcus Vinícius Nora,Alessandra Campbell Pinheiro,Pedro Eduardo Almeida da Silva,Daniela Fernandes Ramos
In the last 15 years, Acinetobacter baumannii has received special attention, mainly due to several resistance mechanisms and high rates of morbimortality. The ability to form biofilms contributes to the persistence of this microorganism in the hospital environment and facilitates the occurrence of nosocomial infections. Several studies have highlighted the pharmacological relevance of pyridines in
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Characterization of VIM-4 Producing Clinical Pseudomonas aeruginosa Isolates from Western Algeria: Sequence Type and Class 1 Integron Description Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Fatma Zohra Zaidi; Radia Dali-Yahia; Karima Zenati; Leila Yazi; Manon Lounes; Salim Aberkane; Helen Jean Pierre; Olivier Barraud; Sylvain Godreuil; Abdelaziz Touati
Objectives:Pseudomonas aeruginosa occupies a central position in nosocomial infections and remains a significant cause of morbidity and mortality. The aim of this study was to characterize carbapenem resistance mechanisms in P. aeruginosa isolates from clinical specimens collected at the University Hospital of Oran, western Algeria.
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Sequence Duplication Within pmrB Gene Contribute to High-Level Colistin Resistance in Avian Pathogenic Escherichia coli. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Jinhu Huang,Xingyang Dai,Lin Ge,Muhammad Shafiq,Jan Mohammad Shah,Junjie Sun,Sida Yi,Liping Wang
Beyond the emergence of plasmid-encoded mechanisms, mutation within the pmrAB genes remains one of the primary colistin resistance mechanisms in Escherichia coli. However, the mechanisms of high-level colistin resistance (HLCR) have not been elucidated. In this study, we evaluated the HLCR mechanisms in five colistin-susceptible Avian pathogenic Escherichia coli (APEC) isolates after colistin exposure
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Trends and Impact in Antimicrobial Resistance Among Bacteroides and Parabacteroides Species in 2007-2012 Compared to 2013-2017. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Marta Kierzkowska,Anna Majewska,Grazyna Mlynarczyk
Purpose: The aim of this study was to analyze the susceptibility of Bacteroides and Parabacteroides spp. strains, isolated from patients hospitalized in the clinical hospital in Poland to penicillin, amoxicillin with clavulanic acid, imipenem, clindamycin, and metronidazole.
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Partial Correlation Between Phenotypic and Genotypic Antimicrobial Resistance of Salmonella enterica Serovar Enteritidis Strains from Brazil. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Fábio Campioni,Carolina N Gomes,Alzira M M Bergamini,Dália P Rodrigues,Juliana P Falcão
Whole-genome sequencing analyses have provided important data and information on the repertoire of resistance genes in several bacterial species. This study examined to what extent the antimicrobial resistance genes found in a set of whole-genome-sequenced Salmonella Enteritidis strains from Brazil correlated with the phenotypic antimicrobial resistance possibly related to these genes. The genotypic
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Phenotypic and Genotypic Characterization of Resistance to Macrolides and Lincosamides in Streptococcus agalactiae Isolated from Pregnant Women in Misiones, Argentina. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Marina Novosak,Fernando Bobadilla,Osvaldo Delgado,Marta Vergara,Margarita Laczeski
The aim of this work was to determine the susceptibility, molecular profile, and clonal relationship in Streptococcus agalactiae (group B Streptococcus [GBS]) isolated from vaginal-rectal swab samples. We worked with 200 isolates collected from pregnant women between 35 and 37 weeks of gestation. The macrolide–lincosamide–streptogramin B (MLSB) resistance phenotypes were determined using the double-disc
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Antibiotic Use Associated with Confirmed Influenza, Pertussis, and Nontyphoidal Salmonella Infections. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Wen-Qiang He,Martyn D Kirk,Vitali Sintchenko,John J Hall,Bette Liu
Purpose: Antibiotics are not the recommended treatment for uncomplicated influenza or nontyphoidal salmonella infections, whereas they are for current pertussis infection. We investigated adherence to these recommendations in a population of older community-dwelling adults.
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Prevalence and Mechanisms of Carbapenem Resistance in Klebsiella pneumoniae and Escherichia coli: A Systematic Review and Meta-Analysis of Cross-Sectional Studies from Iran. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Mohammad Javad Nasiri,Mehdi Mirsaeidi,Seyyed Mohammad Javad Mousavi,Mania Arshadi,Fatemeh Fardsanei,Behnaz Deihim,Sara Davoudabadi,Samin Zamani,Bahareh Hajikhani,Hossein Goudarzi,Mehdi Goudarzi,Zahra Sadat Seghatoleslami,Hossein Dabiri,Payam Tabarsi
Introduction: Carbapenem-resistant Enterobacteriaceae (CRE) represents an urgent threat worldwide. We aimed to investigate the frequency of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli in Iran.
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Chromosome and Plasmid Features of Two ST37 Clostridioides difficile Strains Isolated in China Reveal Distinct Multidrug Resistance and Virulence Determinants. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Junyan Liu,Liang Peng,Heng Su,Haixian Tang,Dingqiang Chen,Zhenbo Xu,Aiwu Wu
Clostridioides difficile ST37 is an emerging and prevalent multilocus sequence type and represents a lineage of clinical significance. This study aimed to characterize two epidemic C. difficile ST37 strains, CD161 and CDT4. CD161 acquires a chromosome and two distinct plasmids, pCD161-L, sharing high similarity with Clostridium phage, and pCD161-S, while CDT4 has a chromosome and a plasmid pCDT4 identical
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Susceptibility of Nitrofurantoin and Fosfomycin Against Outpatient Urinary Isolates of Multidrug-Resistant Enterococci over a Period of 10 Years from India Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 DasArghya; BanerjeeTuhina; AnupurbaShampa
With the increasing emergence of drug resistance in enterococci, there have been very limited data on the efficacy of orally available nitrofurantoin and fosfomycin on enterococci causing urinary tract infections (UTIs), particularly for multidrug-resistant (MDR) strains. This study aimed to determine the in vitro effectiveness of these two drugs against the MDR enterococci. A total of 514 phenotypically
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Molecular Epidemiology and Resistance Profile of Campylobacter jejuni and Campylobacter coli Strains Isolated from Different Sources in Brazil Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 GomesCarolina N.; FrazãoMiliane R.; PassagliaJaqueline; DuqueSheila S.; MedeirosMarta I. Cazentini; FalcãoJuliana P.
Aims: The objectives of this study were to genotype a total of 48 Campylobacter jejuni and 39 Campylobacter coli strains isolated in Brazil from 1995 to 2016 by multilocus sequence typing (MLST) and to determine their resistance profile. The presence or points of mutation in the related resistance genes was verified.
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Virulence, Resistance, and Genomic Fingerprint Traits of Vibrio cholerae Isolated from 12 Species of Aquatic Products in Shanghai, China Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Huiyu Fu; Pan Yu; Weili Liang; Biao Kan; Xu Peng; Lanming Chen
Vibrio cholerae is a waterborne bacterium and can cause epidemic cholera disease worldwide. Continuous monitoring of V. cholerae contamination in aquatic products is imperative for assuring food safety. In this study, we determined virulence, antimicrobial susceptibility, heavy metal tolerance, and genomic fingerprints of 370 V. cholerae isolates recovered from 12 species of commonly consumed aquatic
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High Prevalence of Fecal Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Among Newly HIV-Diagnosed Adults in a Community Setting in Tanzania Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Joel Manyahi; Sabrina John Moyo; Marit Gjerde Tellevik; Nina Langeland; Bjørn Blomberg
Colonization in HIV-infected populations with extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) is particularly worrisome in low-income settings. This study describes the prevalence of ESBL-PE carriage and associated risk factors among newly HIV-diagnosed adults in a community setting in Tanzania. A total of 595 newly diagnosed HIV adults with a median age of 35 years with interquartile
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Resistance to Antiseptics and Disinfectants of Planktonic and Biofilm-Associated Forms of Corynebacterium striatum. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Cassius de Souza,Higor Franceschi Mota,Yuri Vieira Faria,Fellipe de Oliveira Cabral,Dryelle Rodrigues de Oliveira,Lincoln de Oliveira Sant'Anna,Prescilla Emy Nagao,Cintia da Silva Santos,Lílian Oliveira Moreira,Ana Luíza Mattos-Guaraldi
Disinfection and antisepsis are of primary importance in controlling nosocomial infections and outbreaks by pathogens expressing multiple resistance to antimicrobial agents (multidrug-resistant [MDR]) used in therapy. Nowadays, infections related to health services (HAIs) due to MDR and multidrug-susceptible (MDS) Corynebacterium striatum should not be underestimated, including patients using invasive
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Pharmacokinetic/Pharmacodynamic Modeling of Seven Antimicrobials for Empiric Treatment of Adult Bloodstream Infections with Gram-Negative Bacteria in China Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 WangCuicui; HaoWei; JinYan; ShenCuihua; WangBo
Objective: Optimal dosing regimens for achieving a positive clinical outcome were simulated for seven antibiotics commonly used to treat bloodstream infections (BSIs) in adults.
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Potential Inhibitors Against NDM-1 Type Metallo-β-Lactamases: An Overview. Microb. Drug Resist. (IF 2.296) Pub Date : 2020-12-03 Smriti Sharma,Sumit Sharma,Parvinder Pal Singh,Inshad Ali Khan
A new member of the class metallo-β-lactamase (MBL), New Delhi metallo-beta-lactamase 1 (NDM-1) has emerged recently as a leading threat to the treatment of infections that have spread in all major Gram-negative pathogens. The enzyme inactivates antibiotics of the carbapenem family, which are a mainstay for the treatment of antibiotic-resistant bacterial infections. This review provides information
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Bacteria with a Potential for Multidrug Resistance in Hospital Material Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-24 Sandra Geane Pereira de Souza; Isabela Carvalho dos Santos; Maria Augusta Dorigan Bondezan; Laisa Fernanda Melhado Corsatto; Isabel Cristina da Silva Caetano; Melissa Marchi Zaniolo; Rosana da Matta; Luiz Sérgio Merlini; Lidiane Nunes Barbosa; Daniela Dib Gonçalves
The objective of this research was to determine the antimicrobial resistance of bacteria isolated from items related to hygiene and antisepsis, equipment, and instruments used in different hospital wards. Bacterial isolation and identification, phenotypic antimicrobial susceptibility assays, mecA gene detection, and multiple antimicrobial resistance index analysis were performed. In total, 105 bacteria
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Epidemiology, Risk Factors, and Clinical Outcomes of Bloodstream Infection due to Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Hematologic Malignancy: A Retrospective Study from Central South China Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-24 Ting Liang; Cong Xu; Qian Cheng; Yishu Tang; Huiqi Zeng; Xin Li
Objective: To determine the epidemiology, risk factors, and prognosis of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae bloodstream infections (BSIs) among hematology malignancy (HM) patients in China.
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Emergence of Imipenem Resistance in a CpxA-H208P-Variant-Producing Proteus mirabilis Clinical Isolate Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-24 Marion Lecuru; Marie-Hélène Nicolas-Chanoine; Sébastien Tanaka; Philippe Montravers; Laurence Armand-Lefevre; Erick Denamur; Hedi Mammeri
The Proteus mirabilis PmirS clinical isolate, which was susceptible to imipenem (0.5 μg/mL) and amikacin (1 μg/mL), was recovered from a bronchial aspirate of a patient who recently underwent lung transplantation. The P. mirabilis PmirR clinical isolate, which exhibited resistance to imipenem (16 μg/mL) and amikacin (24 μg/mL), was isolated 3 weeks later from the same patient and the same specimen
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Staphylococcus aureus and Its Effects on the Prognosis of Bronchiectasis Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-24 Katheryne Benini Martins; Daniel Wenceslau Votto Olmedo; Milene Machado Paz; Daniela Fernandes Ramos
Bronchiectasis, which is an abnormal and irreversible dilation of one or several bronchial segments, causes significant morbidity and impaired quality of life to patients, mainly as the result of recurrent and chronic respiratory infections. Staphylococcus aureus is a microorganism known for its high infectious potential related to the production of molecules with great pathogenic power, such as enzymes
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A Multidrug-Resistant Salmonella Infantis Clone Is Spreading and Recombining in the United States Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-24 Gregory H. Tyson; Cong Li; Lucas B. Harrison; Gordon Martin; Chih-Hao Hsu; Heather Tate; Thu-Thuy Tran; Errol Strain; Shaohua Zhao
Recently, there have been reports worldwide of a multidrug-resistant, emergent Salmonella Infantis (ESI) clone with a large megaplasmid (pESI), often containing the extended-spectrum beta-lactamase gene blaCTX-M-65. This clone also has a gyrA mutation conferring fluoroquinolone resistance, further limiting treatment options. In the United States, this clone has also been found in poultry sources, indicating
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Characterization of blaKPC-2: Harboring Klebsiella pneumoniae Isolates and Mobile Genetic Elements from Outbreaks in a Hospital in Ecuador Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-20 Jorge Reyes; Paúl Cárdenas; Rafael Tamayo; Fernando Villavicencio; Ana Aguilar; Roberto G. Melano; Gabriel Trueba
Aim: To investigate the mobile genetic elements harboring blaKPC gene in carbapenem-resistant Klebsiella pneumoniae recovered during a 6-month outbreak in a high-complexity hospital from Ecuador.
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Emergence of blaNDM-5 in Enterobacteriaceae Isolates from Companion Animals in Guangzhou, China Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-20 Jing Wang; Ying-Bi Xia; Xin-Yi Huang; Yan Wang; Lu-Chao Lv; Qing-Qing Lin; Meng-Ying Yi; Pei-Lan Lu; Jian–Hua Liu; Zhen-Ling Zeng
The occurrence and characterization of carbapenemase-producing Enterobacteriaceae from companion animals in Guangzhou, China, are investigated. Six isolates (2.3%, 6/257) were positive for blaNDM-5, that is, one Enterobacter cloacae, one Citrobacter freundii, and four Escherichia coli. Three E. coli isolates obtained from the same animal hospital were ST410 and showed identical pulse field gel electrophoresis
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Diversity of Carbapenem Resistance Mechanisms in Clinical Gram-Negative Bacteria in Pakistan Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-18 Linda Hadjadj; Muhammad Ali Syed; Shahid Ahmad Abbasi; Jean-Marc Rolain; Bushra Jamil
Antibiotic resistance is a health challenge worldwide. Carbapenem resistance in Gram-negative bacteria is a major problem since treatment options are very limited. Tigecycline and colistin are drugs of choice in this case, but resistance to these drugs is also high. The aim of this study was to describe the diversity of resistance mechanisms in carbapenem-resistant clinical Gram-negative bacteria from
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Differential Isoniazid Response Pattern Between Active and Dormant Mycobacterium tuberculosis Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-18 M.A. Abo-Kadoum; Yongdong Dai; Mohammed Asaad; Insaf Hamdi; Jianping Xie
Isoniazid (isonicotinic acid hydrazide, INH) is an effective frontline antituberculosis drug. INH targets several Mycobacterium tuberculosis processes, including mycolic acid biosynthesis, DNA synthesis, and redox potential. M. tuberculosis responds to INH stress by altering the expression level of crucial genes involved in various pathways. In this study, we summarize the induced gene expression pattern
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Identification and Characterization of Multidrug-Resistant Extended-Spectrum Beta-Lactamase-Producing Bacteria from Healthy and Diseased Dogs and Cats Admitted to a Veterinary Hospital in Brazil Microb. Drug Resist. (IF 2.296) Pub Date : 2020-11-13 Ricardo Antonio Pilegi Sfaciotte; Leandro Parussolo; Fernanda Danielle Melo; Paula Wildemann; Giseli Bordignon; Naiara Dognani Israel; Marta Leitzke; Sheila Rezler Wosiacki; Fabiano Zanini Salbego; Ubirajara Maciel da Costa; Sandra Maria Ferraz
The objective of this study was to identify the main extended-spectrum beta-lactamase (ESBL)-producing bacteria and to detect the frequency of the major genes responsible to trigger this resistance in hospitalized animals. We collected 106 rectal swabs from cats (n = 25) and dogs (n = 81) to detect ESBL-producing isolates. ESBL-positive samples were submitted to the antimicrobial susceptibility test