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Optimization of limonin invertase production by scaling up aspergillus tabingensis UA13 fermentation to a 5-liter scale. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-30 Yuting Chen,Dandan Yang,Nongfei Ouyang,Shengjiao Lei,Haiyan Liu,Yiwei Huang,Junqian Wu
The enzymatic approach is a highly effective and the major scientific method to eliminating bitter components in citrus-derived products nowadays. Microbial production of limonin invertase stands out due to its pivotal role in the removal of the bitter substance, limonin. The optimization of fermentation parameters and the study of scale-up fermentation are imperative for product commercialization
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Transposon-Aided Capture (TRACA) of Plasmids from the Human Gut. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-29 Dan Chen,Yeshi Yin,Yunfei Hu,Linyan Cao,Changhui Zhao,Baiyuan Li
The gut microbiota consists of a vast and diverse assemblage of microorganisms that play a pivotal role in maintaining host health. Nevertheless, a significant portion of the human gut microbiota remains uncultivated. Plasmids, a type of MGE, assume a critical function in the biological evolution and adaptation of bacteria to varying environments. To investigate the plasmids present within the gut
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Genotypic characterization of Streptococcus didelphis causative of fatal infection in white-eared opossums. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-15 Gabriela Merker Breyer,Maria Eduarda Rocha Jacques da Silva,Mônica Slaviero,Bruno Albuquerque de Almeida,Emanoelly Machado Sousa da Silva,Victória Regina de Queiroz Schmidt,Marcelo Alievi,Grazieli Maboni,Saulo Petinatti Pavarini,Franciele Maboni Siqueira
Streptococcus didelphis was reported once as related to severe infections in opossums. Thus, we present the first comprehensive whole-genome characterization of clinical S. didelphis strains isolated from white-eared opossums (Didelphis albiventris). Long-read whole-genome sequencing was performed using the MinION platform, which allowed the prediction of several genomic features. We observed that
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Comparison of chitosan and SLN nano-delivery systems for antibacterial effect of tea tree (Melaleuca alternifolia) oil against P. aeruginosa and S. aureus. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Hasti Vase,Mehran Nemattalab,Masoumeh Rohani,Zahra Hesari
Treatment of wounds is challenging due to bacterial infections, including Staphylococcus aureus and Pseudomonas aeruginosa. Using the merits of alternative antimicrobials like tea tree oil (TTO) and nanotechnology, they can be helpful in combatting bacterial infections. Solid lipid nanoparticle (SLN) and chitosan (CS) nanoparticles show great potential as carriers for enhancing the stability and therapeutic
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Single versus dual inoculation with indigenous Saccharomyces cerevisiae strains in winemaking. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Ioanna Chalvantzi,Athanasios Mallouchos,Georgios Banilas,Aspasia Nisiotou
Indigenous Saccharomyces cerevisiae strains and their combinations may be used to diversify wines and add complexity to sensory profiles. Here, two S. cerevisiae strains that represent regional genetic and phenotypic specificities for two major winegrowing areas of Greece were used in single- and mixed-culture fermentations. The kinetics and metabolic activities of the strains were analyzed to evaluate
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Retraction of: wbeT sequence typing and IS1004 profiling of Vibrio cholerae isolates. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01
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Development of PMA-qPCR assay to accurately and reproducible quantify viable bacteria of Paenibacillus polymyxa. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Jiacai Guo,Fei Fan,Weiliang Wang,Minxi Wan,Yuanguang Li
Paenibacillus polymyxa is an important biocontrol bacterium. The combination of propidium monoazide (PMA) and quantitative polymerase chain reactionq (qPCR) has proven effective in quantifying live bacteria from various microorganisms. The objective was to create a PMA-qPCR assay to precisely and consistently measure the number of living bacteria of biocontrol P. polymyxa. The primers were designed
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Antimicrobial resistance of Escherichia coli in the UK: comparison of single vs. pooled samples from healthy pigs. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Iñaki Deza-Cruz,María J Vilar,Martina Velasova,Manal AbuOun,Muna F Anjum,Richard P Smith
This study compared the antimicrobial resistance (AMR) of Escherichia coli detected from single samples vs. pooled samples at herd level. The national monitoring dataset included isolates from one sample per pig holding, whereas the research study included isolates from pooled samples of 10 pigs per holding. In both datasets, caecal samples were collected from healthy pigs randomly selected at slaughterhouses
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Antibacterial, bacteriolytic, antibiofilm, and synergistic effects of the peel oils of Citrus microcarpa and Citrus x amblycarpa with tetracycline against foodborne Escherichia coli. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Abdi W Septama,Yuandani Yuandani,Nur A Khairunnisa,Halimah R Nasution,Dinda S Utami,Rhesi Kristiana,Ibrahim Jantan
Citrus essential oils (EOs) have shown significant antibacterial activity. The present study was undertaken to evaluate the antibacterial activity of the peel oils of Citrus microcarpa and C. x amblycarpa against Escherichia coli. The minimum inhibition concentration (MIC) was determined by using the broth microdilution assay. The checkerboard method was used to identify synergistic effects of the
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Streptomyces griseoincarnatus strain RB7AG promotes Oryza sativa (var. swarna) growth under salt stress: mechanisms and potential applications. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Subhransu Sekhar Behera,Suchismita Nivedita,Pratyush Kumar Behera,Zahra Parwez,Seemon Giri,Sourav Ranjan Parida,Lopamudra Ray
The plant growth promoters (PGP) are the natural fertilizers that enhance the overall growth of the plant. We defined Streptomyces strain RB7AG as a potential halotolerant growth promoter and assessed its impact on rice plants' performance under salt stress. The organism was able to thrive at concentrations up to 10% of NaCl (w/v), optimal at 6% as measured by their cell growth, viability, and secondary
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Evaluation of TIB Molbiol LightMix® assays for detection of Mycoplasma genitalium and key resistance mutations for macrolides and fluoroquinolones. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Prisha Balgovind,Steph Atchison,Jennifer Danielewski,Suzanne M Garland,Anna-Maria Costa,Kaveesha Bodiyabadu,Gerald L Murray
The LightMix® Modular Mycoplasma Macrolide and LightMix® Modular parC Fluoroquinolone Resistance assays (TIB Molbiol) were evaluated using sequential Mycoplasma genitalium positive (n = 125) and negative (n = 93) clinical samples. Results were compared to the results of an established commercial assay (ResistancePlus MG assay, SpeeDx Pty Ltd) or Sanger sequencing (for parC). Detection of M. genitalium
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Establishment of real-time fluorescence and visual LAMP for rapid detection of Escherichia coli O157:H7 and kits construction. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-11-01 Zuwei Wang,Xinping Cui,Antuo Hu,Zhaoxin Lu,Fanqiang Meng,Libang Zhou,Xiaomei Bie
Escherichia coli O157:H7 is a common pathogenic bacterium in food and water that can pose a threat to human health. The aim of this study was to develop loop-mediated isothermal amplification (LAMP) method for the detection of E. coli O157:H7 in food based on the specific gene Ecs_2840 and to construct rapid detection kits based on the established methods. Specifically, we established two methods of
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Efficacy of a membrane concentration method combined with real-time PCR for detection of Giardia and Cryptosporidium in drinking water. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Jiang Jingyi,Yao Ping,Xu Jian,Chen Jia,Mao Xujian,Li Qiong,Tu Bowen,Wang Fengming
The occurrence of Giardia and Cryptosporidium (oo)cysts in drinking source water poses a serious public health risk. Here, we established a method that combines membrane concentration and real-time polymerase chain reaction (PCR) to quantify Giardia and Cryptosporidium in drinking water. The water samples were filtered through a cellulose membrane to collect Giardia and Cryptosporidium, and then nucleic
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Pathology, virulence-associated gene profiling, antimicrobial susceptibility, and pathogenicity of untypeable capsular serotypes of Pasteurella multocida isolated from slaughtered pigs of India. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Monalisa Sahoo,Suraj Baloni,Jigarji C Thakor,Pradeep Kumar,Prasad Thomas,Viswas K Nagaleekar,Kuldeep Dhama,Rajendra Singh,Karam P Singh,Saminathan Mani,Salauddin Qureshi,Ajay Kumar,Shailesh K Patel,Jitendra K Biswal,Nihar R Sahoo
Pasteurella multocida is widely distributed in all pig-rearing countries, affecting the economic viability and profitability of pig production. The present research highlights the molecular characterization and pathology of untypeable capsular serotypes of P. multocida in slaughtered pigs from prominent pig-rearing states of India. The prevalence of Pasteurellosis was 27.17% by Pasteurella multocida
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A comparative analysis of quantitative detection methods for viable food-borne pathogens using RT-qPCR and PMA-qPCR. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Guoxiong Wang,Xinyin Nie,Lihong Yang,Hongmei Liao
The accurate quantification of viable pathogens in food is crucial for ensuring food safety. This study mainly aimed to investigate the quantification of viable pathogens using PMA-qPCR and RT-qPCR, taking into account bacterial species, food matrices, and inactivation methods. The detection limit of PMA-qPCR for Salmonella serovars in simple matrices, such as culture broth, lake, or tap water, was
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Antibiofilm activity of promethazine, deferiprone, and Manuka honey in an ex vivo wound model. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Gláucia Morgana de Melo Guedes,Alyne Soares Freitas,Rodrigo Machado Pinheiro,Vinicius Carvalho Pereira,Carliane Melo Alves Melgarejo,Emanuela Silva de Araujo,Késia Veras Costa Ribeiro,Silviane Praciano Bandeira,Rossana de Aguiar Cordeiro,Marcos Fábio Gadelha Rocha,José Júlio Costa Sidrim,Débora de Souza Collares Maia Castelo-Branco
This study evaluated the antibiofilm activity of promethazine, deferiprone, and Manuka honey against Staphylococcus aureus and Pseudomonas aeruginosa in vitro and ex vivo in a wound model on porcine skin. The minimum inhibitory concentrations (MICs) and the effects of the compounds on biofilms were evaluated. Then, counting colony-forming units (CFUs) and confocal microscopy were performed on biofilms
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Characterization of white rot fungi from wood decayed for lignin degradation. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Yakath Ali Nurul-Aliyaa,Nik Azimatolakma Awang,Masratul Hawa Mohd
The present study was conducted to isolate and identify white rot fungi (WRF) from wood decayed and to determine their ability to produce lignin-modifying enzymes (LMEs), specifically laccase (Lac), lignin peroxidase (LiP), and manganese peroxidase (MnP), on solid and liquid media supplemented with synthetic dyes namely 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), azure B, and phenol
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Biostimulated-sesame sprout extracts as potential agents against Leishmania mexicana. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Karime G Garduño-Félix,Jesús J Rochín-Medina,Carolina C Murua-López,Héctor S López-Moreno,Karina Ramírez
Leishmania mexicana is one of the causal agents of cutaneous leishmaniasis. Current antileishmanial chemotherapeutics have demonstrated adverse side effects; thus, alternative treatments are needed. In this study, we performed in silico and in vitro analyses of the leishmanicidal potential of the most abundant phenolic compounds identified in black sesame sprouts biostimulated with Bacillus clausii
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Diagnosis of invasive pulmonary aspergillosis by lateral flow assay of galactomannan in bronchoalveolar lavage fluid: a meta-analysis of diagnostic performance. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Yingli Cai,Jun Liang,Guangsheng Lu,Yankun Zhan,Jianwei Meng,Zhusheng Liu,Yiming Shao
The performance of lateral flow assay (LFA) in diagnosing invasive pulmonary aspergillosis (IPA) has not been well demonstrated. To address this, we conducted a meta-analysis assessing the overall accuracy of LFA in diagnosing IPA using bronchoalveolar lavage fluid (BALF). Over a systematical search and assessment of bias risk, we calculated the pooled specificity, sensitivity, and area under the receiver
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Specific antioxidant enzymes are involved in the freeze-thawing response of industrial baker's yeasts. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 María F Kronberg,Natalia L Terlizzi,Miguel A Galvagno
In this study, the biochemical basis of resistance to slow freezing and thawing (F-T) stress was explored in two baker yeast industrial strains of Saccharomyces cerevisiae that presented differential tolerance to freezing in order to be in the frozen bakery industry. Strain Y8, used commercially in sweet baking doughs, exhibited greater stress tolerance than Y9, a strain employed in regular doughs
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Comparison of the Standard E TB-Feron ELISA and QuantiFERON-TB Gold PLUS assays: the advantageous use of whole recombinant protein antigens for latent tuberculosis diagnosis. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Da-Gyum Lee,Jihye Kang,Jihee Jung,Taeyoon Kim,Jiyeon Kim,Hyunjin Lee,Junghee Lee,Youngsub Won,Sungweon Ryoo
The laboratory diagnosis of latent tuberculosis is often performed using interferon-gamma release assays. Here, we compared two enzyme-linked immunosorbent assay-based interferon-gamma release assays, namely, the newly developed Standard E TB-Feron enzyme-linked immunosorbent assay (STFE) and the QuantiFERON-TB Gold PLUS assay (QFT-GP), using samples from 155 participants. The STFE is based on using
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A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Abhishek Mewara,Sandhya Khunger,Chayan Sharma,Sivanantham Krishnamoorthi,Shreya Singh,Rakesh Yadav,Sumeeta Khurana,Rakesh Sehgal
We developed a rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for two common intestinal parasites-Entamoeba histolytica and Giardia duodenalis, where early detection may be helpful. The mLAMP assay was optimized for the detection of DNA of E. histolytica (18S rRNA gene) and G. duodenalis (Elongation factor 1 alpha gene) from standard strains by using six specific primers FIP (forward
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Bacteria isolated from explosive contaminated environments transform pentaerythritol tetranitrate (PETN) under aerobic and anaerobic conditions. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-10-04 Helena Avila-Arias,Francy-Carolina Casallas,Ziv Arbeli,Andrea García Gutiérrez,Carlos Andres Fajardo Gomez,Denis Yohana Herrera Castillo,Sandra Carvajal Ramirez,Diana Paola Tamayo-Figueroa,Joaquín Benavides López de Mesa,Fabio Roldan
Pentaerythritol tetranitrate (PETN) is a nitrate ester explosive that may be persistent with scarce reports on its environmental fate and impacts. Our main objective was to isolate and characterize bacteria that transform PETN under aerobic and anaerobic conditions. Biotransformation of PETN (100 mg L-1) was evaluated using mineral medium with (M + C) and without (M - C) additional carbon sources under
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The impact of probiotic cell-free metabolites in MDR Pseudomonas aeruginosa: Antibacterial properties and effect on antibiotic resistance genes expression. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-23 N Mehboudi,H R Rahimi,H Aghaee Bakhtiari,M Alimardani,A Jalili
There is a significant demand for novel antibacterial agents against multidrug-resistant (MDR) gram-negative bacteria. Recently, probiotics have been noted for their antibacterial properties against various pathogens. This study aimed to investigate the effects of probiotic cell-free supernatants on MDR Pseudomonas aeruginosa. Clinical isolates demonstrating the highest degree of antibiotic resistance
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Diversity and epidemiological profile of Pseudomonas aeruginosa from drinking water in Brazil genotyped using multi-locus sequence typing. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Paula Vasconcelos Costa,Janaína Dos Santos Nascimento,Stephen James Forsythe,Marcelo Luiz Lima Brandão
Pseudomonas aeruginosa is a Gram-negative bacillus associated with waterborne diseases. The objective of this study was to determine whether particular P. aeruginosa sequence types (STs) were associated with drinking water contamination in Brazil. This was achieved by searching the Pseudomonas PubMLST database, which contains the records for 8358 strains collected between 1938 and 2023. The majority
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Analysis of the features of 105 confirmed CRISPR loci in 487 Klebsiella variicola. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Yanyan Xi,Jiaxue Zhao,Jiangfeng Zhang,Yuefei Jin,Haiyan Yang,Guangcai Duan,Shuaiyin Chen,Jinzhao Long
Klebsiella variicola, an emerging human pathogen, poses a threat to public health. The horizontal gene transfer (HGT) of plasmids is an important driver of the emergence of multiple antibiotic-resistant K. variicola. Clustered regularly interspersed short palindromic repeats (CRISPR) coupled with CRISPR-associated genes (CRISPR/Cas) constitute an adaptive immune system in bacteria, and can provide
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Prevalence, antibiotic resistance profile, MBLs encoding genes, and biofilm formation among clinical carbapenem-resistant Enterobacterales isolated from patients in Mohammed VI University Hospital Centre, Morocco. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Dilagui Ilham,Loqman Souad,Lamrani Hanchi Asmae,Nayme Kawtar,Timinouni Mohammed,Soraa Nabila
Enterobacterales are frequently a major cause of human infections. The emergence of carbapenem resistance as well as the biofilm formation complicate their management. In this regard, this study aimed to investigate the prevalence, antibiogram, carbapenemase genes, and biofilm production among Enterobacterales. For this purpose, 18 172 clinical specimens from hospitalized patients at Mohammed VI University
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Characterization of rhizobia for beneficial traits that promote nodulation in legumes under abiotically stressed conditions. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Langutani Sanger Khambani,Ahmed Idris Hassen,Karl Rumbold
The growing interest in using rhizobia as inoculants in sustainable agricultural systems has prompted the screening of rhizobia species for beneficial traits that enhance nodulation and nitrogen fixation under abiotic stressed conditions. This study reports phenotypic and phylogenetic characterization of rhizobia strains previously isolated from the root nodules of several indigenous and exotic legumes
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Microbial communities associated with resin canal discoloration in mango fruit. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Muhammad Umar,John P Bowman,Constancio Asis,Cameron McConchie,Alieta Eyles,Roger Stanley,Alistair Gracie
Resin canal discoloration (RCD) severely impacts the fruit quality of mango, diminishes consumer confidence, and reduces sales, but the biological cause is still unclear. Using next-generation sequencing, the overall microbial community composition of RCD+ and visually healthy mango fruits was determined for the first time to examine the possible role of bacterial and fungal pathogens in RCD. The diversity
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Autotrophic growth activity of complete ammonia oxidizers in an upflow biological contact filter for drinking water treatment. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Yuta Ishizaki,Futoshi Kurisu,Hiroaki Furumai,Ikuro Kasuga
Biological filters effectively remove ammonium from drinking water via nitrification. In a pilot-scale upflow biological contact filter (U-BCF), complete ammonia oxidizers (comammox), which are capable of oxidizing ammonia to nitrate in one cell, were more abundant than ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, little information is available on the contribution
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Mobile colistin resistance (mcr) genes and recent developments in colistin resistance detection. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Divya Lakshmanan,Dhamodharan Ramasamy,Veni Subramanyam,Suresh Kumar Saravanan
The peptide antibiotic colistin has been reserved as a last resort antibiotic treatment option for cases where other antibiotics including carbapenems have failed. Recent emergence of colistin resistance and discovery of mobile colistin resistance (mcr) genes, which encode the cell wall modifying phosphoethanolamine transferase enzyme, complicates the issue. The mcr genes have been associated with
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Role of Candida in the bioremediation of pollutants: a review. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Samriti Rana,Shristi Handa,Yadu Aggarwal,Sanjeev Puri,Mary Chatterjee
The population and modernization of society have increased dramatically from past few decades. In order to meet societal expectations, there has been a massive industrialization and resource exploitation. Anthropogenic practices like disposal of hazardous waste, large carbon footprint release variety of xenobiotic substances into the environment, which endanger the health of the natural ecosystem.
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Investigation of virulence factors, phylogenetic grouping, multiple-locus variable number tandem repeat analysis, and antimicrobial susceptibility of E. coli isolated from aborted bovine fetal tissue. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Ediz Kağan Özgen,Berna Yanmaz,Perihan Şerifoğlu Bağatir
Escherichia coli is an important microorganism for cattle breeding. The aim of this study was to investigate the presence of phylogenetic groups, virulence factors, genotyping with multi-locus variable tandem repeat analysis (MLVA), and susceptibility to commonly used antimicrobial agents in E. coli strains isolated from aborted bovine fetal samples. In this study, phylogrouping and various virulence
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Phenotypical and molecular characterization of Acinetobacter spp. isolated from a pharmaceutical facility. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Luiza Vasconcellos,Samara Verly Silva,Luciana Veloso da Costa,Rebeca Vitoria da Silva Lage de Miranda,Cristhiane Moura Falavina Dos Reis,Lygia Maria Paulo da Silva Braga,Claudiane Silva,Greice Conceição,Josiane Mattoso,Igor Barbosa Silva,Stephen J Forsythe,Victor Midlej,Maria Helena Simões Villas Boas,Marcelo Luiz Lima Brandão
Characterizing microorganisms according to different criteria is useful when investigating sources of microbiological contamination in the pharmaceutical industry. The aim of this study was to characterize 38 Acinetobacter baumannii complex strains isolated from a biopharmaceutical industry by 16S rRNA sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS)
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A novel Bacillus sp. with antagonistic activity against a plant pathogen, Fusarium graminearum, and its potential antagonistic mechanism. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Shanrui Shen,Feifan Yu,Xinyi Hao,Jing Chen,Huan Gao,Xiaofang Lai
Fusarium head blight (FHB) is a wheat disease caused by the plant pathogen Fusarium graminearum, which leads to crop yield losses and agricultural economic losses, as well as poses a threat to the environment and human health. Effective biocontrol of F. graminearum is urgent. An antagonistic strain HZ-5 with 59.2% antagonistic activity against F. graminearum in vitro had been isolated from sea mud
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Effects of film-forming Pichia and Candida yeasts on cider and wine as post-fermentation contaminants. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Marilinda Lorenzini,Maria Stella Cappello,Anthony Green,Giacomo Zapparoli
Film-forming yeasts are potential sources of defects in alcoholic beverages. The aim of this study is to assess the growth capacity of Pichia and Candida film-forming yeasts in cider and wine and the effects on their chemical composition. Cider, partially and fully fermented wine were inoculated with strains of C. californica, P. fermentans, P. kluyveri, P. kudriavzevii, P. manshurica, and P. membranifaciens
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Fermentation of araticum, baru, and pequi by-products by probiotic strains: effects on microorganisms, short-chain fatty acids, and bioactive compounds. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-09-01 Fellipe Lopes de Oliveira,Maressa Caldeira Morzelle,Marcia Maria de Souza Moretti,Sabrina Neves Casarotti
Fruit by-products, due to their unique chemical composition containing dietary fibers and bioactive compounds, may favor the growth of probiotic strains. This study evaluated the fermentation of araticum, baru, and pequi by-products using Lactobacillus acidophilus (La-5, LA3, and NCFM) and Bifidobacterium animalis subsp. lactis (Bb-12) probiotic strains. We assessed probiotic viability, short-chain
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Antioxidant and L-asparaginase activities of culturable endophytic fungi from ornamental Dendrobium orchids. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-10 Ru Wei Chua,Keang Peng Song,Adeline Su Yien Ting
This study reports the antioxidant potential and L-asparaginase production of culturable fungal endophytes from Dendrobium orchids in Malaysia. Twenty-nine isolates were screened using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine their free radical scavenging activities and antioxidant capacity (IC50 and AEAC). L-asparaginase production of fungal endophytes was detected by the qualitative
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Diversity of virulence and antibiotic resistance genes expressed in Class A biosolids and biosolids-amended soil as revealed by metatranscriptomic analysis. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Elisa Marie D'Angelo
Class A biosolids is a treated sewage sludge, commonly applied to agricultural fields, home lawns/gardens, golf courses, forests, and remediation sites around the world. This practice is of public and agricultural concern due to the possibility that biosolids contain antibiotic-resistant bacteria and fungal pathogens that could persist for extended periods in soil. This possibility was determined by
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Large-scale preparation of yeast strains expressing condensates derived from a glycolytic enzyme via controlled dissolved oxygen levels under hypoxia. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Yuki Murata,Reina Hirayama,Natsuko Miura,Michihiko Kataoka
Under hypoxia, Saccharomyces cerevisiae forms cytoplasmic condensates composed of proteins, including glycolytic enzymes, that are thought to regulate cellular metabolism. However, the hypoxic conditions required for condensate formation remain unclear. In this study, we developed a 300-mL-scale culture method to produce condensate-forming cells by precisely controlling the dissolved oxygen (DO) level
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Synergistic action of indole-3-carbinol with membrane-active agents against multidrug-resistant Gram-negative bacteria. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Tso-Ping Wang,Tzu-Hui Yeh,Chung-Cheng Lo,Kuan-Hua Lin,Mei-Han Huang,Horng-Ren Lo
The purpose of this study was to evaluate the antimicrobial activity of indole-3-carbinol (I3C) with membrane-active agents, namely carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and ethylenediaminetetraacetic acid (EDTA) against multidrug-resistant (MDR) Gram-negative bacteria and bacterial persisters. The determination of minimal inhibitory concentration (MIC) showed that I3C was effective against
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Biofilm formation in drug-resistant Acinetobacter baumannii and Acinetobacter nosocomialis isolates obtained from a university hospital in Pelotas, RS, Brazil. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Suélen Cavalheiro Amaral,Beatriz Bohns Pruski,Stella Buchhorn de Freitas,Lucas Moreira Dos Santos,Daiane Drawanz Hartwig
This study aimed to investigate the antibiotic resistance and biofilm formation of Acinetobacter calcoaceticus-A. baumannii (ACB) complex isolates recovered from a university hospital in Pelotas, RS, Brazil. The species were confirmed using gyrB multiplex and blaOXA-51-like genes PCR. The presence of the bfmRS virulence gene was evaluated by the PCR, and the isolates were classified based on their
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Exploration of the candidate beneficial bacteria for Penaeus vannamei culture by core microbiome analysis using amplicon sequencing. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Natarajan Lalitha,Berty Samuel Masilamoni Ronald,Murugesan Ananda Chitra,Ashok Kumar Jangam,Vinaya Kumar Katneni,Panjan Nathamuni Suganya,Hemalatha Senthilnayagam,Tuticorin Maragatham Alagesan Senthilkumar,Moturi Muralidhar
Globally, Penaeus vannamei is the vital species in aquaculture production. Beneficial bacterial exploration of gut, sediment, and water were investigated in P. vannamei culture using Illumina Miseq sequencing of 16S RNA V3-V4 hypervariable regions. Predominant phyla identified were Proteobacteria, Tenericutes, Bacteroidetes in gut; Proteobacteria, Bacteroidetes, Planctomycetes in sediment and Cyanobacteria
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Mining of a novel reductase and its application for asymmetric reduction of p-methoxyacetophenone. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Nengqiang Wang,Xiaojun Li
(R)-1-(4-methoxyphenyl) ethanol [(R)-1b] is an essential precursor for the synthesis of aryl propanoic acids' anti-inflammatatory drugs. Biocatalysts for (R)-1b preparation are limited and reductase has problems of low substrate concentration and low conversion rate. As a result, there is a constant need for discovering novel biocatalysts with excellent catalytic performances. In this study, a novel
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Temperature influence on the compression and breakage behaviour of yeast cells. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Achim Overbeck,Stephanie Michel,Ingo Kampen,Arno Kwade
Industrial biotechnology uses microbial cells to produce a wide range of products. While the genetic and molecular properties of these organisms are well understood, less is known about their mechanical properties. Previous work has established a test procedure for single yeast cells using a nanoindentation instrument equipped with a flat-punch probe, which allows single cells (Saccharomyces cerevisiae)
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Mycobacterial contamination in tap and shower waters in Thailand. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Jack Jia Xin Song,Kumiko Oguma
Waterborne disease is increasingly becoming associated with opportunistic premise plumbing pathogens (OPPPs), which can resist residual chlorination, regrow throughout drinking water distribution systems, and colonize premise plumbing. Nontuberculous mycobacteria (NTM) include clinically important species and exert a high burden on healthcare systems. We briefly report a qPCR-based survey of Mycobacterium
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The potential of microalgal sources as coating materials: A case study for the development of biocompatible surgical sutures. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Merve Tokgöz,Çağla Yarkent,Ayşe Köse,Suphi S Oncel
Sutures are considered as surgical materials that form excellent surfaces to integrate the postoperative parts of the body. These materials present suitable platforms for potential bacterial penetrations. Therefore, coating these biomedical materials with biocompatible compounds is seen as a potential approach to improve their properties while avoiding adverse effects. The aim of this study was to
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Polymerase chain reaction for the in vitro detection of the pESI plasmid associated with the globally circulating Salmonella Infantis outbreak strain. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Elizabeth A McMillan,Lari M Hiott,Joao A Carrico,Miguel P Machado,Hannes Pouseele,Charlene R Jackson,Jonathan G Frye
A globally circulating strain of Salmonella enterica serotype Infantis containing the pESI plasmid has increased in prevalence in poultry meat samples and cases of human infections. In this study, a polymerase chain reaction (PCR) protocol was designed to detect the pESI plasmid and confirm the Infantis serotype of Salmonella isolates. Primers were tested bioinformatically to predict specificity, sensitivity
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Quantitative multiplex polymerase chain reaction in copies ml-1 linearly correlates with standard urine culture in colonies ml-1 for urinary tract infection (UTI) pathogens. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Richard A Festa,Mike Opel,Mohit Mathur,Natalie Luke,Laura K S Parnell,Dakun Wang,Xinhua Zhao,Jesus Magallon,Michael Percaccio,David Baunoch
Standard urine culture (SUC) is the current standard method for confirmation of a urinary tract infection (UTI). SUC identifies microorganisms in urine samples and semi-quantifies these as colony-forming units (CFUs) ml-1. In contrast, quantitative multiplex polymerase chain reaction (q-MPCR) is a culture-independent assay in which the microbes are quantified by targeting genomic sequences and reported
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Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Marija Pavlović,Slavić Marinela Šokarda,Marina Ristović,Sanja Stojanović,Aleksandra Margetić,Miloš Momčilović,Zoran Vujčić
The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic
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Imipenem and meropenem influence the Las/Rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Chandrayee Deshamukhya,Bhaskar J Das,Deepjyoti Paul,Debadatta Dhar Chanda,Amitabha Bhattacharjee
The present study was conducted to study the influence of imipenem and meropenem at subinhibitory concentration on the transcriptional response of Las/Rhl quorum-sensing systems in isolates of Pseudomonas aeruginosa. In the present study, six representative carbapenem nonsusceptible clinical isolates of P. aeruginosa were obtained. The agar dilution method was used to determine the minimum inhibitory
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Isolation of new Paraburkholderia strains for polyhydroxybutyrate production. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Corinne Ivaldi,Clothilde Foy,Stéphanie Castex,Alexis Vallée,Caroline Rémond,Ludovic Besaury
Polyhydroxyalkanoates (PHAs) are bioplastics that can serve as substitutes for petroleum-based plastics with the advantages of being biodegradable, biocompatible, and biobased. The microbial production of polyhydroxyalkanoates is generally conducted in the presence of sugar mixes rich in monosaccharides. In this study, molecular and cultural approaches based on forest soils enriched with hydrocarbon
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Fatty acid based antimicrobials from Streptomyces sp. SORS-24, an endophyte isolated from Sonchus oleraceus. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-08-02 Rabia Tanvir,Imran Sajid,Yasir Rehman,Shahida Hasnain
Due to the rise in bacterial resistance towards various therapeutic agents, interest is now developing towards fatty acid based antimicrobials because of their non-specific mode of action. A strain SORS 24 isolated from Sonchus oleraceus (Sow thistle) showed significant activity against Escherichia coli ATCC 25922 (25 mm), Chlorella vulgaris (20 mm), Bacillus subtilis DSM 10 (ATCC 6051) and Pseudomonas
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Survival of different microbial strains in pure and diluted tattoo inks. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Rossella Briancesco,Stefania Paduano,Rosa Paradiso,Maurizio Semproni,Lucia Bonadonna
Several microorganisms can be found in tattoo inks injected into the skin, despite the ink matrix being considered inhospitable to microbial growth. Studies on the microbial quality of tattoo inks have reported the presence of microorganisms in most of the samples. This study aimed to assess the survival of environmental and human microbial species, selected on the specific criteria, in tattoo inks
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The inhibitory effect of copper, zinc, and manganese on Legionella longbeachae and other Legionella spp. in vitro. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Hyunwoo Jun,Amy Scott-Thomas,Sandy Slow,Jonathan Williman,David R Murdoch,Stephen T Chambers
Legionella longbeachae is an important cause of Legionnaires' disease in Australasia and is associated with exposure to potting soils. Our aim was to identify ways to reduce the load of L. longbeachae in potting soils. Inductively-coupled plasma optical emission spectrometry (ICP-OES) of an all-purpose potting mix showed copper (Cu) concentrations (mg/kg) range from 15.8 to 23.6. Zinc (Zn) and manganese
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Screening and characterization of agarolytic bacteria from different sources. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Dinesh Labade,Heena Tabassum,Minal Wani
According to the results of our investigation, distinct bacterial isolates capable of breaking down agar were found in various nonmarine environments. The deficiency of reducing sugar in the control media demonstrates that the agar in the experiment is broken down by the bacteria to produce various oligosaccharides because the viscosity of the medium containing the agar was found to have been extremely
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Cross-method assessment of coliphage detection methods in the framework of EPA regulatory standards. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Matthew T Price,Angelia D Blackwood,Rachel T Noble
This study aimed to understand the performance and utility of US EPA-approved coliphage methods in comparison to fecal indicator bacteria (FIB) and molecular microbial source tracking (MST) methods in recreational waters. We used US EPA Method 1642 to quantify concentrations of coliphage along with culture- and molecular-based enumeration of E. coli and Enterococcus sp, and human fecal source marker
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Control of Listeria monocytogenes in a fresh cheese using aromatic and medicinal plants and enterocin: a comparative study. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Samir Ananou,Meryem Bouraqqadi,Nada Zouhri,Said El Kinany,Laila Manni
The aim of this study was to investigate the effectiveness of essential oils (EOs) or crude extracts (CEs) of eight aromatic and medicinal plants (AMPs) and its association with enterocin OS1 on Listeria monocytogenes and food spoilage bacteria in Moroccan fresh cheese. The cheese batches were treated with EO of Rosmarinus officinalis, Thymus vulgaris, Syzygium aromaticum, Laurus nobilis, Allium sativum
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Improved functional properties of the potential probiotic Lacticaseibacillus paracasei ItalPN16 growing in cheese whey. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 Cristian Mauricio Barreto Pinilla,Adriano Brandelli,Fabiana Galland,Leila Maria Spadoti,Adriana Torres Silva E Alves
The production of probiotic bacteria requires specific and expensive culture media for maintain their viability and metabolic response during gastro-intestinal transit and cell adhesion process. The aim of this study was to compare the ability of the potential probiotic Laticaseibacillus paracasei ItalPN16 to grow in plain sweet whey (SW) and acid whey (AW), evaluating changes in some probiotic properties
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Controlling Fusarium wilt of cape gooseberry by microbial consortia. Lett. Appl. Microbiol. (IF 2.4) Pub Date : 2023-07-03 DianaS García,Adriana González-Almario,Alba M Cotes
The use of microbial consortia has become a promising alternative for the management of various diseases. In this study, 18 artificial consortia were designed, consisting of five bacteria, five fungi, and a mixture of five fungi and five bacteria; from a collection of microorganisms isolated from the rhizosphere of cape gooseberry plants grown in two soils potentially suppressive against Fusarium oxysporum