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Phloroglucinol Enhances Anagen Signaling and Alleviates H2O2-Induced Oxidative Stress in Human Dermal Papilla Cells. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-03-08 Seokmuk Park, Ye Jin Lim, Hee Su Kim, Hee-Jae Shin, Ji-Seon Kim, Jae Nam Lee, Jae Ho Lee, Seunghee Bae
Phloroglucinol (PG) is one of the abundant isomeric benzenetriols in brown algae. Due to its polyphenolic structure, PG exhibits various biological activities. However, the impact of PG on anagen signaling and oxidative stress in human dermal papilla cells (HDPCs) is unknown. In this study, we investigated the therapeutic potential of PG for improving hair loss. A non-cytotoxic concentration of PG
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1-kestose blocks UVB-induced skin inflammation and promotes Type I procollagen synthesis via regulating MAPK/AP-1, NF-κB and TGF-β/Smad pathway. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-22 Jihye Baek, Jong-Hwa Kim, Jiwon Park, Do Hyun Kim, Soonok Sa, Jung-Sook Han, Wonyong Kim
Solar UVB irradiation cause skin photoaging by inducing the high expression of matrix metalloproteinase (MMPs) to inhibit the expression of Type1 procollagen synthesis. 1-kestose, a natural trisaccharide, has been indicated to show a cytoprotective role in UVB radiation-induced-HaCaT cells. However, few studies have confirmed the anti-aging effects. In the present study, we evaluated the anti-photoaging
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Advancements in Sustainable Plant-Based Alternatives: Exploring Proteins, Fats, and Manufacturing Challenges in Alternative Meat Production. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-19 Minju Jung, YouKyeong Lee, Sung Ok Han, Jeong Eun Hyeon
The rise in plant-based food consumption is propelled by concerns for sustainability, personal beliefs, and a focus on healthy dietary habits. This trend, particularly in alternative meat, has attracted attention from specialized brands and eco-friendly food companies, leading to increased interest in plant-based alternatives. The dominant plant-based proteins, derived mainly from legumes, include
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Unveiling immunomodulatory effects of Euglena gracilis in immunosuppressed mice: Transcriptome and pathway analysis. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-19 Seon Ha Jo, Kyeong Ah Jo, Soo-Yeon Park, Ji Yeon Kim
The immunomodulatory effects of Euglena gracilis (Euglena) and its bioactive component, β-1,3-glucan (paramylon), have been clarified through various studies. However, the detailed mechanisms of the immune regulation remain to be elucidated. This study was designed not only to investigate the immunomodulatory effects but also to determine the genetic mechanisms of Euglena and β-glucan in cyclophosphamide
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Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-19 Hye Seon Song, Yeon Bee Kim, Joon Yong Kim, Seong Woon Roh, Tae Woong Whon
Despite considerable advancements achieved using next-generation sequencing technologies in exploring microbial diversity, several species of the gut microbiome remain unknown. In this transformative era, culturomics has risen to prominence as a pivotal approach in unveiling realms of microbial diversity that were previously deemed inaccessible. Utilizing innovative strategies to optimize growth and
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Establishment of a Dual-Vector System for Gene Delivery Utilizing Prototype Foamy Virus. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-15 Soo-Yeon Cho, Yoon Jae Lee, Seong-Mook Jung, Young Min Son, Cha-Gyun Shin, Eui Tae Kim, Kyoung-Dong Kim
Foamy viruses (FVs) are generally recognized as non-pathogenic, often causing asymptomatic or mild symptoms in infections. Leveraging these unique characteristics, FV vectors hold significant promise for applications in gene therapy. This study introduces a novel platform technology using a pseudo-virus with single-round infectivity. In contrast to previous vector approaches, we developed a technique
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Improving the Three-Dimensional Printability of Potato Starch Loaded onto Food Ink. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-13 Yourim Oh, Seungmin Lee, Nam Keun Lee, Jin-Kyu Rhee
This study focuses on improving the 3D printability of pea protein with the help of food inks designed for jet-type 3D printers. Initially, the food ink base was formulated using nanocellulose-alginate with a gradient of native potato starch and its 3D printability was evaluated. The 3D-printed structures using only candidates for the food ink base formulated with or without potato starch exhibited
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Optimization of Ultrasound-Assisted Pretreatment for Accelerating Rehydration of Adzuki Bean (Vigna angularis). J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-12 Changgeun Lee, Hyengseop Kim, Eunghee Kim, Youngje Jo, Jiyoon Park, Choongjin Ban, Seokwon Lim
Adzuki bean (Vigna angularis), which provides plant-based proteins and functional substances, requires a long soaking time during processing, which limits its usefulness to industries and consumers. To improve this, ultrasonic treatment using high pressure and shear force was judged to be an appropriate pretreatment method. This study aimed to determine the optimal conditions of ultrasound treatment
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Autolysis of Pseudomonas aeruginosa Quorum-Sensing Mutant Is Suppressed by Staphylococcus aureus through IronDependent Metabolism. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-02 Shin-Yae Choi, In-Young Chung, Hee-Won Bae, You-Hee Cho
Microorganisms usually coexist as a multifaceted polymicrobial community in the natural habitats and at mucosal sites of the human body. Two opportunistic human pathogens, Pseudomonas aeruginosa and Staphylococcus aureus commonly coexist in the bacterial infections for hospitalized and/or immunocompromised patients. Here, we observed that autolysis of the P. aeruginosa quorum-sensing (QS) mutant (lasRmvfR)
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Diet-induced gut dysbiosis and leaky gut syndrome. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-02-01 Yu-Rim Chae, Yu Ra Lee, Young-Soo Kim, Ho-Young Park
Chronic gut inflammation promotes the development of metabolic diseases such as obesity. There is growing evidence which suggests that dysbiosis in gut microbiota and metabolites disrupt the integrity of the intestinal barrier and significantly impact the level of inflammation in various tissues, including the liver and adipose tissues. Moreover, dietary sources are connected to the development of
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Prognostic Value of an Immune Long NonCoding RNA Signature in Liver Hepatocellular Carcinoma. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-30 Rui Kong, Nan Wang, Chun Li Zhou, Jie Lu
In recent years, there has been a growing recognition of the important role that long non-coding RNAs (lncRNAs) play in the immunological process of hepatocellular carcinoma (LIHC). An increasing number of studies have shown that certain lncRNAs hold great potential as viable options for diagnosis and treatment in clinical practice. The primary objective of our investigation was to devise an immune
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Melanin Inhibitory Effect of Tuber himalayense Isolated in Incheon, Korea. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-30 Byeong Min Choi, Minkyeong Kim, Hyehyun Hong, Tae-Jin Park, Changmu Kim, Jin-Soo Park, Won-Jae Chi, Seung-Young Kim
There has been a growing interest in skin beauty and antimelanogenic products. Melanogenesis is the process of melanin synthesis whereby melanocytes are activated by UV light or hormone stimulation to produce melanin. Melanogenesis is mediated by several enzymes, such as tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), and TRP-2. In this
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Intracellular Flux Prediction of Recombinant Escherichia coli Producing Gamma-aminobutyric Acid. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-30 Sung Han Bae, Myung Sub Sim, Ki Jun Jeong, Dan He, Inchan Kwon, Tae Wan Kim, Hyun Uk Kim, Jong-Il Choi
The genome-scale metabolic model (GEM) can be used to simulate cellular metabolic phenotypes under various environmental or genetic conditions. This study utilized the GEM to observe the internal metabolic fluxes of recombinant Escherichia coli producing gamma-aminobutyric acid (GABA). Recombinant E. coli was cultivated in a fermenter under three conditions: pH 7, pH 5, and additional succinic acids
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Lactobacillus Persisters Formation and Resuscitation. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-29 Hyein Kim, Sejong Oh, Sooyeon Song
Lactobacillus is widely used as a probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not Lactobacillus. Thus, understanding Lactobacillus persister cells will provide essential clues for
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A Narrative Review on the Advance of Probiotics to Metabiotics. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-29 Hye Ji Jang, Na-Kyoung Lee, Hyun-Dong Paik
Recently, the term metabiotics has emerged as a new concept of probiotics. This concept entails combining existing probiotic components with metabolic by-products improve specific physiological functionalities. Representative ingredients of these metabiotics include short-chain fatty acids (SCFAs), bacteriocins, polysaccharides, and peptides. The new concept is highly regarded as it complements the
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Genomic Characterization and Safety Assessment of Bifidobacterium breve BS2- PB3 as Functional Food. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-25 Kristin Talia Marbun, Marcelia Sugata, Jonathan Suciono Purnomo, Dikson, Samuel Owen Mudana, Tan Tjie Jan, Juandy Jo
Our group had isolated Bifidobacterium breve strain BS2-PB3 from human breast milk. In this study, we sequenced the whole genome of B. breve BS2-PB3, and with a focus on its safety profile, various probiotic characteristics (presence of antibiotic resistance genes, virulence factors, and mobile elements) were then determined through bioinformatic analyses. The antibiotic resistance profile of B. breve
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Secretory production of the Hericium erinaceus laccase from Saccharomyces cerevisiae. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-22 Jin Kang, Thuat Van La, Mi-Jin Kim, Jung-Hoon Bae, Bong Hyun Sung, Seonghun Kim, Jung-Hoon Sohn
Mushroom laccases play a crucial role in lignin depolymerization, one of the most critical challenges in lignin utilization. Importantly, laccases can utilize a wide range of substrates, such as toxicants and antibiotics. This study isolated a novel laccase, named HeLac4c, from endophytic white-rot fungi Hericium erinaceus mushrooms. The cDNAs for this enzyme were 1569 bp in length and encoded a protein
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Taxonomic Variations of Bacterial and Fungal Communities Depending on Fermentation Temperature in Traditional Korean Fermented Soybean Food, Doenjang. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-19 Eunhye Jo, Hyeyoung Lee, Younshil Song, Jaeho Cha
Meju, a fermented soybean brick, is a key component in soybean foods like doenjang and ganjang, harboring a variety of microorganisms, including bacteria and fungi. These microorganisms significantly contribute to the nutritional and sensory characteristics of doenjang and ganjang. Amplicon-based next-generation sequencing was applied to investigate how the microbial communities of meju fermented at
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Oral administration of Alcohol-Tolerant Lactic Acid Bacteria Alleviates Blood Alcohol Levels and Ethanol-Induced Liver Damage in Rodents. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-18 Misun Yun, Hee Eun Jo, Namhee Kim, Hyo Kyeong Park, Young Seo Jang, Ga Hee Choi, Ha Eun Jo, Seo Jeong Hyun, Ji Ye Mok, Sang Min Park, Hak-Jong Choi
Excessive alcohol consumption can have serious negative consequences on health, including addiction, liver damage, and other long-term effects. The causes of hangovers include dehydration, alcohol and alcohol metabolite toxicity, and nutrient deficiency due to absorption disorders. Additionally, alcohol consumption can slow reaction times, making it more difficult to rapidly respond to situations that
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Understanding a Core Pilin of the Type IVa Pili of Acidithiobacillus thiooxidans, PilV. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-17 Araceli Hernández-Sánchez, Edgar D Páez-Pérez, Elvia Alfaro-Saldaña, Vanesa Olivares-Illana, J Viridiana García-Meza
Pilins are protein subunits of pili. The pilins of type IV pili (T4P) in pathogenic bacteria are well characterized, but anything is known about the T4P proteins in acidophilic chemolithoautotrophic microorganisms such as the genus Acidithiobacillus. The interest in T4P of A. thiooxidans is because of their possible role in cell recruitment and bacterial aggregation on the surface of minerals during
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In vitro anti-inflammatory and skin protective effects of Codium fragile extract on macrophages and human keratinocytes in atopic dermatitis. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-17 A-Yeong Jang, JeongUn Choi, Weerawan Rod-In, Ki Young Choi, Dae-Hee Lee, Woo Jung Park
Codium fragile has been traditionally used in oriental medicine to treat enterobiasis, dropsy, and dysuria, and it has been shown to possess many biological properties. Atopic dermatitis (AD) is one of the types of skin inflammation and barrier disruption, which leads to chronic inflammatory skin diseases. In the current investigation, the protective effects of C. fragile extract (CFE) on anti-inflammation
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Molecular and Phenotypic Investigation on Antibacterial Activities of Limonene Isomers and Its Oxidation Derivative against Xanthomonas oryzae pv. Oryzae. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-17 Hyeonbin Kim, Mi Hee Kim, Ui-Lim Choi, Moonsoo Chung, Chul-Ho Yun, Youngkun Shim, Jaejun Oh, Sungbeom Lee, Gun Woong Lee
Xanthomonas oryzae pv. oryzae (Xoo) causes a devastating bacterial leaf blight in rice. Here, the antimicrobial effects of D-limonene, L-limonene, and its oxidative derivative carveol against Xoo were investigated. We revealed that carveol treatment at ≥ 0.1 mM in liquid culture resulted in significant decrease in Xoo growth rate (> 40%) in a concentration-dependent manner, and over 1 mM, no growth
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Anti-Inflammatory Activity of Vacuum Distillate from Panax ginseng Root on LPS-Induced RAW264.7 Cells. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-13 Chanwoo Lee, Seul Lee, Young Pyo Jang, Junseong Park
Panax ginseng has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of Panax ginseng distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on l
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Ozonated Sunflower Oil (OSO) alleviates inflammatory responses in oxazolone-induced atopic dermatitis (AD)-like mice and LPS- treated RAW 264.7 cells. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-12 Su-Young Kim, Jung Ok Lee, Sue Lee, Jihye Heo, Kyung-Hyun Cho, Ashutosh Bahuguna, Kwang-Ho Yoo, Beom Joon Kim
Ozone, a highly reactive oxidant molecule, is widely used as a complementary therapy for various skin diseases, including wound healing, pressure ulcers, diabetic foot, and infections. However, there is limited research on the effectiveness of ozone for atopic dermatitis (AD). Ozonated sunflower oil (OSO) is an active ingredient obtained from partially ozonated sunflower oil (SO). OSO markedly reduced
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Fisetin-Mediated Perturbations of Membrane Permeability and Intracellular pH in Candida albicans. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-10 Younhee Kim
The antifungal activity of fisetin against Candida albicans is explored, elucidating a mechanism centered on membrane permeabilization and ensuing disruption of pH homeostasis. The Minimum Inhibitory Concentration (MIC) of fisetin, indicative of its interaction with the fungal membrane, increases in the presence of ergosterol. Hoechst 33342 and propidium-iodide staining reveal substantial propidium-iodide
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Heat-Killed Enterococcus faecium KU22001 Having Effective Anti-Cancer Effects on HeLa Cell Lines at a Lower Temperature. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-08 Jun-Su Ha, Na-Kyoung Lee, Hyun-Dong Paik
The anti-cancer effects of heat-killed Enterococcus faecium KU22001 (KU22001), KU22002, and KU22005 isolated from human infant feces were investigated. The anti-proliferative activity of these strains against various cancer cell lines was evaluated using the MTT assay. To determine the production of exopolysaccharides (EPS) with potential anti-cancer effect, ethanol precipitation and phenol-sulfuric
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Inhibition of Polyphosphate Degradation in Synechocystis sp. PCC6803 through Inactivation of the phoU Gene. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2024-01-08 Han-Bin Ryu, Mi-Jin Kang, Kyung-Min Choi, Il-Kyu Yang, Seong-Joo Hong, Choul-Gyun Lee
Phosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis
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Seasonal Change of Sediment Microbial Communities and Methane Emission in Young and Old Mangrove Forests in Xuan Thuy National Park. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-30 Cuong Tu Ho, Unno Tatsuya, Son Giang Nguyen, Thi-Hanh Nguyen, Son Truong Dinh, Son Tho Le, Thi-Minh-Hanh Pham
Microbial communities in mangrove forests have recently been intensively investigated to explain the ecosystem function of mangroves. In this study, the soil microbial communities under young (<11 years-old mangroves) and old (>17 years-old) mangroves have been studied during dry and wet seasons. In addition, biogeochemical properties of sediments and methane emission from the two different mangrove
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Gromwell (Lithospermum erythrorhizon) Attenuates High-Fat-Induced Skeletal Muscle Wasting by Increasing Protein Synthesis and Mitochondrial Biogenesis. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-30 Ji-Sun Kim, Hyunjung Lee, Ahyoung Yoo, Hang Yeon Jeong, Chang Hwa Jung, Jiyun Ahn, Tae-Youl Ha
Gromwell (Lithospermum erythrorhizon, LE) can mitigate obesity-induced skeletal muscle atrophy in C2C12 myotubes and high-fat diet (HFD)-induced obese mice. The purpose of this study was to investigate the anti-skeletal muscle atrophy effects of LE and the underlying molecular mechanism. C2C12 myotubes were pretreated with LE or Shikonin, and active component of LE, for 24 h and then treated with 500
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Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-29 Ahyeon Cho, Alpana Joshi, Hor-Gil Hur, Ji-Hoon Lee
Root-nodule nitrogen-fixing bacteria are known for being specific to particular legumes. This study isolated the endophytic root-nodule bacteria from the nodules of legumes and examined them to determine whether they could be used to promote the formation of nodules in other legumes. Forty-six isolates were collected from five leguminous plants and screened for housekeeping (16S rRNA), nitrogen fixation
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Synbiotic of Pediococcus acidilactici and Inulin Ameliorates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-27 Mingzhu Wang, Longzhou Zhang, Huiyan Piao, Yuanming Jin, Chengdu Cui, Xin Jin, Lianhua Cui, Chunri Yan
Colitis is a major gastrointestinal disease that threatens human health. In this study, a synbiotic composed of inulin and Pediococcus acidilactici (P. acidilactici) was investigated for its ability to alleviate dextran sulfate sodium (DSS)-induced colitis. The results revealed that the synbiotic, composed of inulin and P. acidilactici, attenuated the body weight loss and disease activity index (DAI)
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Specific Alternation of Gut Microbiota and the Role of Ruminococcus gnavus in the Development of Diabetic Nephropathy. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-26 Jinni Hong, Tingting Fu, Weizhen Liu, Yu Du, Junmin Bu, Guojian Wei, Miao Yu, Yanshan Lin, Cunyun Min, Datao Lin
In this study, we aim to investigate the precise alterations in the gut microbiota during the onset and advancement of diabetic nephropathy (DN) and examine the impact of Ruminococcus gnavus (R. gnavus) on DN. Eight-week-old male KK-Ay mice were administered antibiotic cocktails for a duration of two weeks, followed by oral administration of R. gnavus for an additional eight weeks. Our study revealed
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Enzymatic Activity and Amino Acids Production of Predominant Fungi from Traditional Meju During Soybean Fermentation. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-26 Dong Hyun Kim, Byung Hee Chun, Jae-Jung Lee, Oh Cheol Kim, Jiye Hyun, Dong Min Han, Che Ok Jeon, Sang Hun Lee, Sang-Han Lee, Yong-Ho Choi, Seung-Beom Hong
To investigate the effect of the predominant fungal species from Korean traditional meju and doenjang on soybean fermentation, the enzymatic activity and amino acid production of twenty-two fungal strains were assessed through solid- and liquid-state soybean fermentation. Enzymatic activity analyses of solid-state fermented soybeans revealed different enzyme activities involving protease, leucine aminopeptidase
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Transcriptional Response of Pectobacterium carotovorum to Cinnamaldehyde Treatment. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-25 Jihye Jung, Dawon Jo, Soo-Jin Kim
Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown
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Nasal Immunization Using Chitosan Nanoparticles with Glycoprotein B of Murine Cytomegalovirus. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-22 Marcela Slovakova, Sylva Janovska, Radek Sleha, Vera Radochova, Alexandra Hatala, Nikola Mannova, Radovan Metelka, Ludovit Pudelka, Pavel Bostik
The use of nanoparticles as a delivery system for a specific antigen could solve many limitations of mucosal vaccine applications, such as low immunogenicity, or antigen protection and stabilization. In this study, we tested the ability of nasally administered chitosan nanoparticles loaded with glycoprotein B of murine cytomegalovirus to induce an immune response in an animal model. The choice of chitosan
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Antimicrobial Cyclic Dipeptides from Japanese Quail (Coturnix japonica) Eggs Supplemented with Probiotic Lactobacillus plantarum. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-19 Sa-Ouk Kang, Min-Kyu Kwak
Fifteen cyclic dipeptides (CDPs) containing proline, one cyclo(Phe-Ala) without proline, and a non-peptidyl DL-3-phenyllactic acid were previously identified in the culture filtrates of Lactobacillus plantarum LBP-K10, an isolate from kimchi. In this study, we used Japanese quail (Coturnix japonica) eggs to examine the effects of probiotic supplementation on the antimicrobial CDPs extracted from quail
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Antioxidant and Anti-Obesity Effects of Juglans mandshurica in 3T3-L1 Cells and High-Fat Diet Obese Rats. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-18 Da-Hye Choi, Min Hong, Tae-Hyung Kwon, Soo-Ung Lee
Juglans mandshurica Maxim. walnut (JMW) is well-known for the treatment of dermatosis, cancer, gastritis, diarrhea, and leukorrhea in Korea. However, the molecular mechanism underlying its anti-obesity activity remains unknown. In the current study, we aimed to determine whether JMW can influence adipogenesis in 3T3-L1 preadipocytes and high-fat diet rats and determine the antioxidant activity. The
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Aptamer Based SPREETA Sensor for the Detection of Porphyromonas gingivalis G-Protein. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-18 Suk-Gyun Park, Hyun Ju Lee, Taeksoo Ji, Kyungbaek Kim, Seung-Ho Ohk
We have developed an aptamer that specifically binds to Porphyromonas gingivalis to reduce the cellular damage caused by P. gingivalis infection and applied it as a biosensor. P. gingivalis is one of the major pathogens causing destructive periodontal disease among the periodontal microorganisms constituting complex biofilms. PGP (Porphyromonas gingivalis G-protein) known to play an important role
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Change in the Gut Microbiota of Lactating Sows and Their Piglets by Inclusion of Dietary Spray-Dried Plasma in Sow Diets. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-15 Jeong Jae Lee, Hyunjin Kyoung, Jin Ho Cho, Kyeong Il Park, Yonghee Kim, Jinmu Ahn, Jeehwan Choe, Younghoon Kim, Hyeun Bum Kim, Minho Song
This study aimed to investigate the effects of dietary spray-dried plasma (SDP) on the gut microbiota of lactating sows and their piglets. A total 12 sows were randomly assigned to one of two dietary treatment groups in a completely randomized design. The treatments were a sow diet based on corn and soybean meal (CON), and a CON diet with an added 1% SDP. The sows were fed the dietary treatments from
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Coffee Husk By-Product as Novel Ingredients for Cascara Kombucha Production. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-08 Bao Xuyen Nguyen Le, Thach Phan Van, Quang Khai Phan, Gia Bao Pham, Hoa Pham Quang, Anh Duy Do
Kombucha, a fermented beverage, is gaining popularity due to its numerous beneficial health effects. Various substrates such as herbs, fruits, flowers, and vegetables, have been used for kombucha fermentation in order to enhance the flavor, aroma, and nutritional composition. This study aims to investigate the potential suitability of cascara as a novel ingredient for kombucha production. Our findings
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Production of ρ-Hydroxyacetophenone by Engineered Escherichia coli Heterologously Expressing 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-08 Wenmei Wu, Xiwei Yuan, Xin Gao, Chaoyang Tan, Shunxiang Li, Dehong Xu
ρ-Hydroxyacetophenone is an important and versatile compound that has been widely used in medicine, cosmetics, new materials, and other fields. At present, there are two ways to obtain ρhydroxyacetophenone. One is to extract it from plants, such as Artemisia capillaris Thunb and Cynanchum otophyllum Schneid, and the other is to synthesize it by using chemical methods. Of these two methods, the second
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Hepatoprotective Effect of Lactiplantibacillus plantarum DSR330 in Mice with High Fat Diet-Induced Nonalcoholic Fatty Liver Disease. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-08 Na-Kyoung Lee, Yunjung Lee, Da-Soul Shin, Jehyeon Ra, Yong-Min Choi, Byung Hee Ryu, Jinhyeuk Lee, Eunju Park, Hyun-Dong Paik
Lactiplantibacillus plantarum DSR330 (DSR330) has been examined for its antimicrobials production and probiotics. In this study, the hepatoprotective effects of DSR330 were examined against non-alcoholic fatty liver disease (NAFLD) in a high-fat diet (HFD)-fed C57BL/6 mouse model. To induce the development of fatty liver, a HFD was administered for five weeks, and then silymarin (positive control)
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Optimizing Conditions for the Production of Bacterial Extracellular Vesicles of Vibrio vulnificus and Analysis of the Inner Small RNA Compositions. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-05 JeongHeon Park, Suji Song, Soyee Kim, Minjeong Kim, Kun-Soo Kim
Chemical and physical elements affecting the production of bacterial extracellular vesicles (BEVs) of the human pathogen Vibrio vulnificus were quantitatively assessed to optimize the conditions for the BEV production by using the western blot quantification for an outer membrane porin OmpU and by fluorescent dye FM4-64. When cells were cultured at 37oC in an enriched medium (2Luria Bertani; 2LB)
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A Replication-Competent Retroviral Vector Expressing the HERV-W Envelope Glycoprotein is a Potential Tool for Cancer Gene Therapy. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-01 Byoung Kwon Kang, Yong-Tae Jung
The fusogenic membrane glycoprotein (FMG) derived from the human endogenous retrovirus-W (HERV-W) exhibits fusogenic properties, making it a promising candidate for cancer gene therapy. When cells are transfected with HERV-W FMG, they can fuse with neighboring cells expressing the receptor, resulting in the formation of syncytia. These syncytia eventually undergo cell death within a few days. In addition
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LPS-Induced Modifications in Macrophage Transcript and Secretion Profiles Are Linked to Muscle Wasting and Glucose Intolerance. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-01 Heeyeon Ryu, Hyeon Hak Jeong, Seungjun Lee, Min-Kyeong Lee, Myeong-Jin Kim, Bonggi Lee
Macrophages are versatile immune cells that play crucial roles in tissue repair, immune defense, and the regulation of immune responses. In the context of skeletal muscle, they are vital for maintaining muscle homeostasis but macrophage-induced chronic inflammation can lead to muscle dysfunction, resulting in skeletal muscle atrophy characterized by reduced muscle mass and impaired insulin regulation
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Production of Mono-Hydroxylated Derivatives of Terpinen-4-ol by Bacterial CYP102A1 Enzymes. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-12-01 Jeong-Hoon Kim, Chan Mi Park, Hae Chan Jeong, Gyeong Han Jeong, Gun Su Cha, Sungbeom Lee, Chul-Ho Yun
CYP102A1 from Bacillus megaterium is an important enzyme in biotechnology, because engineered CYP102A1 enzymes can react with diverse substrates and produce human cytochrome P450-like metabolites. Therefore, CYP102A1 can be applied to drug metabolite production. Terpinen-4-ol is a cyclic monoterpene and the primary component of essential tea tree oil. Terpinen-4-ol was known for therapeutic effects
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Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-30 Hyun Jin Kim, Sion Ham, Nara Shin, Jeong Hyeon Hwang, Suk Jin Oh, Tae-Rim Choi, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang
Indigo is a valuable, natural blue dye that has been used for centuries in the textile industry. The large-scale commercial production of indigo relies on its extraction from plants and chemical synthesis. Studies are being conducted to develop methods for environment-friendly and sustainable production of indigo using genetically engineered microbes. Here, to enhance the yield of bioindigo from an
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Innate Immune-Enhancing Effect of Pinus densiflora Pollen Extract via NF-κB Pathway Activation. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-29 Sehyeon Jang, San Kim, Se Jeong Kim, Jun Young Kim, Da Hye Gu, Bo Ram So, Jung A Ryu, Jeong Min Park, Sung Ran Yoon, Sung Keun Jung
Considering the emergence of various infectious diseases, including the coronavirus disease 2019 (COVID-19), people's attention has shifted towards immune health. Consequently, immune-enhancing functional foods have been increasingly consumed. Hence, developing new immune-enhancing functional food products is needed. Pinus densiflora pollen can be collected from the male red pine tree, which is commonly
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Overproduction of xanthophyll Pigment in Flavobacterium sp. JSWR-1 under Optimized Culture Conditions. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-24 Jegadeesh Raman, Young-Joon Ko, Jeong-Seon Kim, Da-Hye Kim, Soo-Jin Kim
Flavobacterium can synthesize xanthophyll, particularly the pigment zeaxanthin, which has significant economic value in nutrition and pharmaceuticals. Recently, the use of carotenoid biosynthesis by bacteria and yeast fermentation technology has shown to be very efficient and offers significant advantages in large-scale production, cost-effectiveness, and safety. In the present study, JSWR-1 strain
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In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-20 Jae Min Hwang, Mun-Hoe Lee, Yuri Kwon, Hee-Chul Chung, Do-Un Kim, Jin-Hee Lee
This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally
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Serotype-Dependent Inhibition of Streptococcus pneumoniae Growth by Short-Chain Fatty Acids. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-20 Suwon Lim, Dongwook Lee, Sungho Jeong, Jeong Woo Park, Jintaek Im, Bokeum Choi, Donghyun Gwak, Cheol-Heui Yun, Ho Seong Seo, Seung Hyun Han
Streptococcus pneumoniae (pneumococcus) is an opportunistic pathogen that can cause severe infectious diseases such as pneumonia, meningitis, and otitis media. Despite the availability of antibiotics and pneumococcal vaccines against some invasive serotypes, pneumococcal infection remains a tremendous clinical challenge due to the increasing frequency of infection by antimicrobial resistant, nonencapsulated
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Bioactivity-Guided Fraction from Viscera of Abalone, Haliotis discus hannai Suppresses Cellular Basophils Activation and Anaphylaxis in mice. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-20 Kap Seong Choi, Tai-Sun Shin, Ginnae Ahn, Shin Hye Kim, Jiyeon Chun, Mina Lee, Dae Heon Kim, Han-Gil Choi, Kyung-Dong Lee, Sun-Yup Shim
Basophils and mast cells are specialized effector cells in allergic reactions. Haliotis discus hannai (abalone), is valuable seafood. Abalone male viscera, which has a brownish color and has not been previously reported to show anti-allergic activities, was extracted with acetone. Six different acetone/hexane fractions (0, 10, 20, 30, 40, and 100%) were obtained using a silica column via βhexosaminidase
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Combined Non-Thermal Microbial Inactivation Techniques to Enhance the Effectiveness of Starter Cultures for Kimchi Fermentation. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-20 Su-Ji Kim, Sanghyun Ha, Yun-Mi Dang, Ji Yoon Chang, So Yeong Mun, Ji-Hyoung Ha
For quality standardization, the application of functional lactic acid bacteria (LAB) as starter cultures for food fermentation is a well-known method in the fermented food industry. This study assessed the effect of adding a non-thermally microbial inactivated starter culture to kimchi, a traditional Korean food, in standardizing its quality. In this study, pretreatment based on sterilization processes
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Steamed Ginseng Berry Powder Ameliorates Skeletal Muscle Atrophy via Myogenic Effects. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-17 Jungbum Kim, Hui-Ji Choi, Donghyuk Seo, Sang-Ah Lee, Jong Beom Heo, Dong Hyuk Baek, Wonhwa Lee, Gyu Yong Song
Sarcopenia is an age-related loss of muscle mass and function for which there is no approved pharmacological treatment. We tested direct efficacy by evaluating grip strength improvement in a sarcopenia mouse model rather than drug screening, which inhibits specific molecular mechanisms. Various physiological functions of ginseng berries are beneficial to the human body. The present study aimed to evaluate
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Molecular and Biochemical Characterization of Xylanase Produced by Streptomyces viridodiastaticus MS9, a Newly Isolated Soil Bacterium. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-17 Jong-Hee Kim, Won-Jae Chi
A xylan-degrading bacterial strain, MS9, was recently isolated from soil samples collected in Namhae, Gyeongsangnam-do, Republic of Korea. This strain was identified as a variant of Streptomyces viridodiastaticus NBRC13106T based on 16S rRNA gene sequencing, DNA-DNA hybridization analysis, and other chemotaxonomic characteristics, and was named Streptomyces viridodiastaticus MS9 (=KCTC29014=DSM42055)
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Establishment and Characterization of Immortalized Human Dermal Papilla Cells Expressing Human Papillomavirus 16 E6/E7. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-17 Seonhwa Kim, Kyeong-Bae Jeon, Hyo-Min Park, Jinju Kim, Chae-Min Lim, Do-Young Yoon
Primary human dermal papilla cells (HDPCs) are often preferred in studies on hair growth and regeneration. However, primary HDPCs are limited by their reduced proliferative capacity, decreased hair induction potential, and extended doubling times at higher passages. To overcome these limitations, pTARGET vectors containing human papillomavirus16 (HPV16) E6/E7 oncogenes were transfected into HDPCs and
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Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from Geobacillus thermoglucosidasius SKF4. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-15 Suleiman D Allison, Nur AdeelaYasid, Fairolniza Mohd Shariff, Nor'Aini Abdul Rahman
Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification
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Effect of Resveratrol and Curcumin on Gene Expression of Methicillin-Resistant Staphylococcus aureus (MRSA) Toxins. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-15 Areej M El-Mahdy,Maisa Alqahtani,May Almukainzi,Majed F Alghoribi,Shaymaa H Abdel-Rhman
Staphylococcus aureus is an opportunistic pathogen that can lead to a number of potentially terrible community- and hospital-acquired illnesses. Among the diverse set of virulence factors that S. aureus possesses, secreted toxins play a particularly preeminent role in defining its virulence. In this work, we aimed to facilitate the development of novel strategies utilizing natural compounds to lower
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The 80th Threonine Residue of Histone H3 Is Important for Maintaining HM Silencing in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-15 Soojin Yeom,Junsoo Oh,Donghyun Kim,Jung-Shin Lee
Gene expression in eukaryotic cells is intricately regulated by chromatin structure and various factors, including histone proteins. In Saccharomyces cerevisiae, transcriptionally silenced regions, such as telomeres and homothallic mating (HM) loci, are essential for genome stability and proper cellular function. We firstly observed the defective HM silencing in alanine substitution mutant of 80th
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Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways. J. Microbiol. Biotechnol. (IF 2.8) Pub Date : 2023-11-14 Hyo Lim Lee, Jong Min Kim, Min Ji Go, Seung Gyum Joo, Tae Yoon Kim, Han Su Lee, Ju Hui Kim, Jin-Sung Son, Ho Jin Heo
This study evaluated the hepatoprotective effect of fermented Protaetia brevitarsis larvae (FPB) in ethanol-induced liver injury mice. As a result of amino acids in FPB, 18 types of amino acids including essential amino acids were identified. In the results of in vitro tests, FPB increased alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities. In addition, FPB treatment increased