-
Comparison of whole genomes of tick-borne encephalitis virus from mountainous alpine regions and regions with a lower altitude Virus Genes (IF 1.991) Pub Date : 2021-01-24 G. Lemhöfer, L. Chitimia-Dobler, G. Dobler, M. Bestehorn-Willmann
Tick-borne encephalitis (TBE) has been a notifiable disease in Germany since 2001. Its causative agent, the TBE virus (TBEV), is the most important arbovirus in Europe and Northern Asia. The illness, caused by the European Subtype usually displays flu-like symptoms, but can result in sequelae and, in 2 % of all cases, in death. Over the last few decades, the virus has spread into new habitats, such
-
Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548 Virus Genes (IF 1.991) Pub Date : 2021-01-20 Wenjing Zhang, Rongrong Zhang, Yunjia Hu, Yujie Liu, Liqin Wang, Xiaoping An, Lihua Song, Taoxing Shi, Huahao Fan, Yigang Tong, Hui Liu
Stenotrophomonas maltophilia (hereinafter referred to as S. maltophilia) has developed into an important opportunistic pathogenic bacterium, which is prevalent in nosocomial and community infections, and has adverse effects on patients with a compromised immune system. Phage vB_SmaS_BUCT548 was isolated from sewage of Beijing 307 Hospital with S. maltophilia (strain No.824) as a host. Phage morphology
-
Removal of 3C protease from the 3ABC improves expression, solubility, and purification of the recombinant 3AB of foot-and-mouth disease virus Virus Genes (IF 1.991) Pub Date : 2021-01-05 Reda Salem, Alaa A. El-Kholy, Fatma R. Waly, Radwa Khaled, Wael Elmenofy
During an ongoing outbreak of Foot-and-Mouth Disease Virus (FMDV), it is crucial to distinguish naturally infected from vaccinated seropositive animals. This would support clinical assessment and punctual vigilance. Assays based on 3ABC non-structural protein as an antigen are reliable for this intention. However, the insolubility and degradation of recombinant 3ABC during expression and purification
-
In vitro inhibitory effect of maraviroc on the association of the simian immunodeficiency virus envelope glycoprotein with CCR5 Virus Genes (IF 1.991) Pub Date : 2021-01-05 Ignacio Giraudy, César A. Ovejero, José L. Affranchino, Silvia A. González
Asian macaques infected with simian immunodeficiency viruses (SIVs) isolated from African non-human primates develop a disease similar to human AIDS. SIV enters its target cells by binding to CD4 and a coreceptor, typically CCR5. Maraviroc is an entry inhibitor of human immunodeficiency virus type 1 (HIV-1) that prevents the interaction between CCR5 and the surface subunit gp120 of the viral envelope
-
Full-length genome characterization of a novel alphapartitivirus detected in the ectomycorrhizal fungus Hygrophorus penarioides Virus Genes (IF 1.991) Pub Date : 2021-01-03 Ergin Sahin, Ilgaz Akata
Virus populations of ectomycorrhizal fungi remain poorly studied. In the present study, we characterized a new partitivirus isolated from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides, named “Hygrophorus penarioides partitivirus 1” (HpPV1). The whole genome of HpPV1, determined by merging deep sequencing and RLM-RACE approaches, comprised two dsRNA segments of 2053 bp and 2072 bp
-
Effects of β-HPV on DNA damage response pathways to drive carcinogenesis: a review Virus Genes (IF 1.991) Pub Date : 2021-01-03 Danyal Tahseen, Peter L. Rady, Stephen K. Tyring
The DDR is a complex signaling network responsible for the preservation of genomic integrity. Beta human papillomaviruses (β-HPVs) are able to destabilize the host genome by attenuating the DDR machinery at the molecular scale following expression of the oncogenes E6 and E7. In the event of β-HPV infection, the E6- and E7-mediated inhibition of the DDR enhances the oncogenicity of UV-induced mutations
-
Peste des petits ruminants virus non-structural C protein inhibits the induction of interferon-β by potentially interacting with MAVS and RIG-I Virus Genes (IF 1.991) Pub Date : 2021-01-03 Li Linjie, Shi Xiaoling, Ma Xiaoxia, Cao Xin, Amjad Ali, Bai Jialin
Peste des petits ruminants virus (PPRV) causes an acute and highly contagious disease in domestic and wild small ruminants throughout the world, mainly by invoking immunosuppression in its natural hosts. It has been suggested that the non-structural C protein of PPRV helps in evading host responses but the molecular mechanisms by which it antagonizes the host responses have not been fully characterized
-
PK15 cell line stably overexpressing IL2 enhances PCV2 replication Virus Genes (IF 1.991) Pub Date : 2021-01-03 Xuliang Zhang, Yingfeng Zhao, Chang Ma, Jinwei You, Min Dong, Shifeng Yun, Jie Liu
Porcine circovirus type 2 (PCV2) is the primary agent responsible for porcine circovirus-associated diseases (PCVADs), which is acknowledged as one of the most economically important diseases for the swine industry worldwide. Currently, the development of PCV2 vaccine against PCVADs and for other applications require large amounts of viral particles. The low propagation rate of PCV2 in vitro limits
-
Diversity of tomato-infecting begomoviruses and spatiotemporal dynamics of an endemic viral species of the Brazilian Atlantic rain forest biome Virus Genes (IF 1.991) Pub Date : 2020-11-24 Macária F. Duarte, Maria E. N. Fonseca, Hélcio Costa, Niday A. N. Fernandes, Ailton Reis, Leonardo S. Boiteux, Rita C. Pereira-Carvalho
Yield losses induced by a complex of begomoviruses are observed across all major tomato-producing areas in Brazil. Tomato severe rugose virus (ToSRV) is the most widespread begomovirus in the country. Conversely, tomato common mosaic virus (ToCmMV) displays a more restricted geographical distribution to areas associated with the Atlantic Rain Forest (ARF) biome, encompassing the States of Espírito
-
Molecular interactions of plant viral satellites Virus Genes (IF 1.991) Pub Date : 2020-11-23 Uzma Badar, Srividhya Venkataraman, Mounir AbouHaidar, Kathleen Hefferon
Plant viral satellites fall under the category of subviral agents. Their genomes are composed of small RNA or DNA molecules a few hundred nucleotides in length and contain an assortment of highly complex and overlapping functions. Each lacks the ability to either replicate or undergo encapsidation or both in the absence of a helper virus (HV). As the number of known satellites increases steadily, our
-
First identification and genomic characterization of equine hepacivirus sub-type 3 strain in China. Virus Genes (IF 1.991) Pub Date : 2020-09-22 Liyan Wu,Jiajun Ou,Siqi Cai,Jinzhao Ji,Zixin Ren,Ran Shao,Shoujun Li
Equine Hepacivirus (EqHV) is a newly discovered equine virus that is classified under the Hepacivirus genus of the Flaviviridae family. There are three sub-types of EqHV worldwide namely; sub-types 1–3. The majority of EqHV sub-type 1 strains were found in China. While different sub-types have been found in Japan and USA, therefore, to investigate whether the other sub-types of EqHV strains were present
-
Tomato yellow vein streak virus and Tomato golden vein virus : a reappraisal of the classification status of two South American Begomovirus species based upon genome–wide pairwise identity of multiple isolates Virus Genes (IF 1.991) Pub Date : 2020-11-19 Luciane de Nazaré Almeida dos Reis, Leonardo S. Boiteux, Maria Esther N. Fonseca, Rita de Cássia Pereira–Carvalho
Tomato yellow vein streak virus (ToYVSV) and tomato golden vein virus (TGVV) are begomoviruses reported infecting tomatoes and other hosts across South America. However, their close phylogenetic relationship has generated uncertainties about their taxonomic status and nomenclature. In fact, genomic DNA–A identity levels of isolates reported with an identical virus name may range from 89–100%. In view
-
Identification of a reptile lyssavirus in Anolis allogus provided novel insights into lyssavirus evolution Virus Genes (IF 1.991) Pub Date : 2020-11-07 Masayuki Horie, Hiroshi Akashi, Masakado Kawata, Keizo Tomonaga
Lyssaviruses (genus Lyssavirus) are negative-strand RNA viruses belonging to the family Rhabdoviridae. Although a lyssa-like virus (frog lyssa-like virus 1 [FLLV-1]), which is distantly related to lyssaviruses, was recently identified in frogs, a large phylogenetic gap exists between those viruses, and thus the evolution of lyssaviruses is unclear. In this study, we detected a lyssa-like virus from
-
Co-infection of a novel fusagravirus and a partitivirus in a Korean isolate of Rosellinia necatrix KACC40168 Virus Genes (IF 1.991) Pub Date : 2020-11-07 Jeesun Chun, Dae-Hyuk Kim
We report here the presence of dsRNA mycoviruses in a Korean isolate of Rosellinia necatrix. A multiple band pattern of double-stranded RNA (dsRNA) from R. necatrix suggested mixed mycovirus infection. Next-generation sequencing analysis of purified dsRNAs indicated the presence of two dsRNA mycoviruses related to the members of families “Fusagraviridae” (proposed) and Partitiviridae. The first dsRNA
-
Enzootic nasal tumor virus type 2 envelope of goats acts as a retroviral oncogene in cell transformation Virus Genes (IF 1.991) Pub Date : 2020-11-05 Naoyoshi Maeda, Yasuo Inoshima, Marcelo De las Heras, Katsumi Maenaka
Enzootic nasal tumor virus type 1 (ENTV-1) (ovine nasal tumor virus) and ENTV-2 (caprine nasal tumor virus) are known to be causative agents of enzootic nasal adenocarcinoma (ENA) in sheep and goats, respectively. Although the nucleotide and amino acid sequences of ENTV-1 and ENTV-2 are quite similar, they are recognized as phylogenetically distinct viruses. The envelope protein of ENTV-1 functions
-
The N-terminal cysteine protease domain of rice stripe tenuivirus Pc1 possesses deubiquitinating enzyme activity Virus Genes (IF 1.991) Pub Date : 2020-11-04 Shuling Zhao, Xiaomei Gu, Jie Li, Changyong Liang
Virus encoded deubiquitinating enzyme (DUB) plays important roles in viral replication and the regulation of host innate immunity. Bioinformatics-based analysis revealed the presence of an ovarian tumor (OTU) protease domain in the N terminus of rice stripe tenuivirus (RSV) Pc1. Many viral OTU domains have been reported to possess DUB activity, which suggests that RSV OTU probably also have DUB activity
-
Co-circulation of genotypes XIV.2 and XVIII.2 of avian paramyxovirus-1 (Newcastle disease virus) in backyard poultry in Niger Virus Genes (IF 1.991) Pub Date : 2020-10-31 Maman Moutari Souley, Abdoulkarim Issa Ibrahim, Bachir Souley Kouato, Alassane Abdou, Rahila Issa, Bachir Yaou, Hadiza Amadou, Hama Hama, Hassane Adakal, Nourou Abdou, Giovanni Cattoli, William G. Dundon
The causative agent of Newcastle disease (ND) of poultry is the avian paramyxovirus-1, also commonly known as ND virus (NDV). Like in many developing countries, ND is endemic in Niger and has significant economic impact on commercial and backyard poultry production. NDVs were characterized in Niger between 2006 and 2008 and shown to belong to genotypes XIV.1 and XVII. In order to determine the current
-
Assessment of replication of bovine herpesvirus type 4 in human glioblastoma and breast cancer cells as a potential oncolytic virus Virus Genes (IF 1.991) Pub Date : 2020-10-26 Touraj Aligholipour Farzani, Seval Bilge Dagalp, Aykut Ozkul, Hakan Gurdal, Firat Dogan, Feray Alkan
Oncolytic viruses have been extensively used in cancer treatment due to their tropism, selective replication only in tumor cells, and possible synergic interaction with other therapeutics. Different researchers have demonstrated that bovine herpesvirus 4 (BoHV-4), a member of the gammaherpesviridae family, has oncolytic potential in some human-origin cancer cell lines like glioma through the selective
-
Whole genome sequencing and phylogenetic classification of Tunisian SARS-CoV-2 strains from patients of the Military Hospital in Tunis Virus Genes (IF 1.991) Pub Date : 2020-10-09 Susann Handrick, Malena Bestehorn-Willmann, Simone Eckstein, Mathias C. Walter, Markus H. Antwerpen, Habiba Naija, Kilian Stoecker, Roman Wölfel, Mohamed Ben Moussa
In the present work, two complete genome sequences of SARS-CoV-2 were obtained from nasal swab samples of Tunisian SARS-CoV-2 PCR-positive patients using nanopore sequencing. The virus genomes of two of the patients examined, a Tunisian soldier returning from a mission in Morocco and a member of another Tunisian family, showed significant differences in analyses of the total genome and single nucleotide
-
Transcriptional programs of infectious spleen and kidney necrosis virus (ISKNV) in vitro and in vivo Virus Genes (IF 1.991) Pub Date : 2020-10-08 Jian-hui He, Lichao Huang, Zhixun Guo, Shaoping Weng, Jianguo He, Xiaopeng Xu
Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR. Transcription of all the putative open reading frames
-
Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs Virus Genes (IF 1.991) Pub Date : 2020-10-08 Jian-hui He, Qiong Xia, Shaoping Weng, Jianguo He, Xiaopeng Xu
MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by complementary binding to target mRNAs. Virus-encoded miRNAs play important roles in virus life cycle and virus-host interactions. Viruses from the Megalocytivirus genus, family Iridoviridae, infect a wide range of fishes, bringing great challenges to aquaculture. Infectious spleen and kidney necrosis
-
Date palm virus A: first plant virus found in date palm trees Virus Genes (IF 1.991) Pub Date : 2020-10-07 Ayoub Maachi, Tatsuya Nagata, João Marcos Fagundes Silva
In this work, a novel ssRNA (+) viral genomic sequence with gene organization typical of members of the subfamily Quinvirinae (family Betaflexiviridae) was identified using high- throughput sequencing data of date palm obtained from the Sequence Read Archive database. The viral genome sequence consists of 7860 nucleotides and contains five ORFs encoding for the replication protein (Rep), triple gene
-
Construction and immune protection evaluation of recombinant virus expressing Newcastle disease virus F protein by the largest intergenic region of fowlpox virus NX10 Virus Genes (IF 1.991) Pub Date : 2020-10-03 Yan Zhao, Zongxi Han, Xiaocai Zhang, Xuemei Zhang, Junfeng Sun, Deying Ma, Shengwang Liu
Fowlpox virus (FPV) is used as a vaccine vector to prevent diseases in poultry and mammals. The insertion site is considered as one of the main factors influencing foreign gene expression. Therefore, the identification of insertion sites that can stably and efficiently express foreign genes is crucial for the construction of recombinant vaccines. In this study, we found that the insertion of foreign
-
Characterization of H3 subtype avian influenza viruses isolated from poultry in Vietnam Virus Genes (IF 1.991) Pub Date : 2020-09-29 Kosuke Soda, Mina Kashiwabara, Kozue Miura, Trang T. H. Ung, Hang L. K. Nguyen, Hiroshi Ito, Mai Q. Le, Toshihiro Ito
To date, avian influenza viruses (AIVs) have persisted in domestic poultry in wet markets in East Asian countries. We have performed ongoing virus surveillance in poultry populations in Vietnam since 2011, with the goal of controlling avian influenza. Throughout this study, 110 H3 AIVs were isolated from 2760 swab samples of poultry in markets and duck farms. H3 hemagglutinin (HA) genes of the isolates
-
Potential reverse spillover of infectious bursal disease virus at the interface of commercial poultry and wild birds. Virus Genes (IF 1.991) Pub Date : 2020-09-24 Rania F El Naggar,Mohammed A Rohaim,Muhammad Munir
Recently, multiple spillover events between domesticated poultry and wild birds have been reported for several avian viruses. This phenomenon highlights the importance of the livestock-wildlife interface in the possible emergence of novel viruses. The aim of the current study was to investigate the potential spillover and epidemiological links of infectious bursal disease virus (IBDV) between wild
-
First molecular identification of porcine circovirus-like agents in dogs and cats in China. Virus Genes (IF 1.991) Pub Date : 2020-09-22 Libin Wen,Aihua Mao,Jianping Xie,Kongwang He
Porcine circovirus-like agents comprise two types of viruses: porcine circovirus-like viruses (P1, P2, P3, and P4) and porcine circovirus-like mini agents (PCVL258, PCVL264, PCVL201, and PCVL347). Of these, P1 has been identified in pigs, cattle, goats, and rabbits in China; P2, P3, P4, PCVL258, and PCVL264 have been identified in pigs; and PCVL201 and PCVL347 have been identified in cattle. The purpose
-
Analysis of host protein interactions in plant viruses: an in silico study using Sesbania mosaic virus. Virus Genes (IF 1.991) Pub Date : 2020-09-19 Mariya Rashid,Shubham Mittal,Sangita Venkataraman
The dynamics of interactions of viral proteins with their host are pivotal in establishing a successful infection and ensuring systemic spread. To uncover these, an in silico analysis of the interactions between the coat protein (CP) of Sesbania mosaic virus (SeMV), a group IV virus with single-stranded positive-sense RNA genome was carried out with the known crystal structures of proteins belonging
-
Subcellular localization of the porcine deltacoronavirus nucleocapsid protein. Virus Genes (IF 1.991) Pub Date : 2020-09-17 Zhen Ding,Suxian Luo,Wang Gong,Leyi Wang,Nengshui Ding,Jun Chen,Jiajia Chen,Ting Wang,Yu Ye,Deping Song,Lingbao Kong,Jinghua Zhang,Yuxing Tang
Porcine deltacoronavirus (PDCoV) has been recently identified as an emerging enteropathogenic coronavirus that mainly infects newborn piglets and causes enteritis, diarrhea and high mortality. Although coronavirus N proteins have multifarious activities, the subcellular localization of the PDCoV N protein is still unknown. Here, we produced mouse monoclonal antibodies against the PDCoV N protein. Experiments
-
Increased viral read counts and metagenomic full genome characterization of porcine astrovirus 4 and Posavirus 1 in sows in a swine farm with unexplained neonatal piglet diarrhea. Virus Genes (IF 1.991) Pub Date : 2020-09-03 Steven Van Borm,Kevin Vanneste,Qiang Fu,Dominiek Maes,Alexandra Schoos,Eline Vallaey,Frank Vandenbussche
Neonatal diarrhea in piglets may cause major losses in affected pig herds. The present study used random high-throughput RNA sequencing (metagenomic next generation sequencing, mNGS) to investigate the virome of sows from a farm with persistent neonatal piglet diarrhea in comparison to two control farms without diarrhea problems. A variety of known swine gastrointestinal viruses was detected in the
-
An antiviral drug-resistant mutant of hepatitis B virus with high replication capacity in association with a large in-frame deletion in the preS1 region of viral surface gene. Virus Genes (IF 1.991) Pub Date : 2020-08-25 Ting Wang,Yanli Qin,Jing Zhang,Xinyan Li,Shuping Tong,Weifeng Zhao,Jiming Zhang
We amplified a full-length hepatitis B virus (HBV) genome from the serum of a chronic hepatitis B patient who experienced virological breakthrough with high HBV DNA titer following adefovir (ADV) therapy. The PCR product was cloned and sequencing of the six clones revealed an isolate of C2 subgenotype. Mutation(s) in the polymerase gene responsible for ADV resistance included rtA181T (all clones) and
-
Identification and characterization of a novel bat polyomavirus in Japan. Virus Genes (IF 1.991) Pub Date : 2020-08-20 Ryota Koba,Satori Suzuki,Go Sato,Shingo Sato,Kazuo Suzuki,Soichi Maruyama,Yukinobu Tohya
A novel polyomavirus (PyV) was identified in the intestinal contents of Japanese eastern bent-wing bats (Miniopterus fuliginosus) via metagenomic analysis. We subsequently sequenced the full genome of the virus, which has been tentatively named Miniopterus fuliginosus polyomavirus (MfPyV). The nucleotide sequence identity of the genome with those of other bat PyVs was less than 80%. Phylogenetic analysis
-
Isolation and phylogenomic analysis of two Senecavirus A isolates in Sichuan Province, 2018. Virus Genes (IF 1.991) Pub Date : 2020-08-19 Kenan Peng,Xinhuan Yin,Yingqi Chen,Zhiwen Xu,Jun Zhao,Jianbo Huang,Chaoyuan Jiang,Ling Zhu
In our study, we isolated and characterised two new Senecavirus A (SVA) isolates in Sichuan Province, China. Phylogenetic analysis of both the SVA full-length genomes and the VP1 genes revealed that the two new SVA isolates are more closely related to previous Chinese strains and US strains. The most variable isolate, SVV-SC-01, showed a significant difference from previous SVA strains, and it was
-
Integrative analysis of microRNA and mRNA expression profiles in MARC-145 cells infected with PRRSV. Virus Genes (IF 1.991) Pub Date : 2020-08-12 Zhenpu Liang,Liang Wang,Hui Wu,Deepali Singh,Xiaoxia Zhang
Porcine reproductive and respiratory syndrome virus (PRRSV) causes tremendous economic losses to the swine industry worldwide. miRNAs are crucial regulators of gene expression and a wide range of complex interactions of miRNAs–mRNAs is possible during virus infection. However, there is no comprehensive integrated study of miRNA and mRNA networks in MARC-145 cells after infection with PRRSV. We analyzed
-
Geographically distant isolates of the persistent southern tomato virus (STV) show very low genetic diversity in the putative coat protein gene. Virus Genes (IF 1.991) Pub Date : 2020-07-31 L Elvira-González,L Rubio,L Galipienso
Southern tomato virus (STV) from genus Amalgavirus (Family Amalgaviridae) is a persistent virus infecting tomato crops worldwide. Information on genetic diversity and evolutionary mechanisms for plant persistent viruses are very scarce in comparison with plant acute viruses. In this work, the putative coat protein gene of worldwide STV isolates was analyzed showing very low nucleotide diversity (< 0
-
Sequencing and phylogenetic characterization of a novel Polerovirus from Nicotiana tabacum. Virus Genes (IF 1.991) Pub Date : 2020-07-30 Yingjie Liu,Yonghao Dong,Xi Chen,Yun Zang,Jinyan Liu,Guangwei Ren,Pengjun Xu
In this study, we reported the complete genome of a novel Polerovirus, named Tobacco yellow virus (TYV), which can be transmitted by Myzus persicae. TYV had a single-stranded RNA genome of 5735 nucleotides in length and contained six putative open reading frames (ORFs). Phylogenetic analysis with whole genome nucleotide sequences and amino acid sequences deduced from the conserved domain of the RNA-dependent
-
Unusual mono-reassortant of a Wa-like G1P[8] species A rotavirus containing a DS-1-like (genotype 2) NSP4 gene. Virus Genes (IF 1.991) Pub Date : 2020-07-22 Tung Phan,Tomihiko Ide,Satoshi Komoto,Pattara Khamrin,Shoko Okitsu,Koki Taniguchi,Hideaki Kikuta,Niwat Maneekarn,Satoshi Hayakawa,Hiroshi Ushijima
Species A rotaviruses are a major cause of acute gastroenteritis in infants and young children worldwide. Reassortment is a common phenomenon due to the segmented nature of the rotavirus genome. The complete coding sequences of a species A rotavirus strain isolated from the feces of a child with acute gastroenteritis in Japan in 2018 were determined using an unbiased viral metagenomics approach. The
-
Molecular characterization of rabies viruses from two western provinces of the Democratic Republic of the Congo (2008-2017). Virus Genes (IF 1.991) Pub Date : 2020-07-21 Georges Tshilenge Mbuyi,Erick Kazadi Kawaya,Augustin T Twabela,Giovanni Cattoli,Julienne Sumbu Walandila,Ivancho Naletoski,Justin Masumu,William G Dundon
Although rabies is enzootic in the Democratic Republic of the Congo, there is very little molecular epidemiological information about the viruses circulating in animals. In this study, a fragment of the rabies virus (RABV) nucleoprotein gene was amplified and sequenced from 21 animal brain samples collected in two western provinces of the country between 2008 and 2017. The samples tested were from
-
Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina. Virus Genes (IF 1.991) Pub Date : 2020-07-20 Verónica Trucco,Dariel Cabrera Mederos,Sergio Lenardon,Fabian Giolitti
Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has
-
Identification of recombination in novel goose parvovirus isolated from domesticated Jing-Xi partridge ducks in South China. Virus Genes (IF 1.991) Pub Date : 2020-07-16 Wen-Jun Liu,You-Tian Yang,Hai-Yin Zou,Shi-Jian Chen,Chen Yang,Yun-Bo Tian,Yun-Mao Huang
Outbreaks of short beak and dwarfism syndrome (SBDS), caused by a novel goose parvovirus (NGPV), have occurred in China since 2015. This rapidly spreading, infectious disease affects ducks in particular, with a high morbidity and low mortality rate, causing huge economic losses. This study analyzed the evolution of NGPV isolated from Jing-Xi partridge duck with SBDS in South China. Complete genome
-
Evidence for zoonotic transmission of species A rotavirus from goat and cattle in nomadic herds in Morocco, 2012-2014. Virus Genes (IF 1.991) Pub Date : 2020-07-10 Sanaâ Alaoui Amine,Marouane Melloul,Moulay Abdelaziz El Alaoui,Hassan Boulahyaoui,Chafiqa Loutfi,Nadia Touil,Elmostafa El Fahime
Species A rotaviruses (RVAs) are a leading cause of diarrhea in children and in the young of a large variety of mammalian and avian host species. The purpose of this study was to identify RVA in nomadic goats and calves during severe diarrhea outbreaks in 2012 and 2014 in Bouaarfa, Morocco, and to characterize the complete genomic constellation of two bovine and caprine strains (S18 and S19) and their
-
Genomic characterization of a novel bovine papillomavirus type 28. Virus Genes (IF 1.991) Pub Date : 2020-07-07 Nanako Yamashita-Kawanishi,Masano Tsuzuki,Fumi Kasuya,Hui-Wen Chang,Takeshi Haga
Infection of bovine papillomavirus (BPV) has been associated with mucosal and/or cutaneous tumor development in bovids. To date, up to 27 genotypes of BPVs have been identified and classified based on the nucleotide sequence identity of L1 open reading frame. In the present study, the complete sequence of a novel BPV concurrently identified with BPV1 and BPV2 in the facial cutaneous papilloma lesion
-
Correction to: Evolutionary and recombination analysis of porcine reproductive and respiratory syndrome isolates in China. Virus Genes (IF 1.991) Pub Date : 2020-06-27 Zhendong Zhang,Xiangyang Qu,Hongliang Zhang,Xudong Tang,Ting Bian,Yingjun Sun,Mingming Zhou,Fubo Ren,Ping Wu
The original version of this article unfortunately contained an error in GenBank Accession Number.
-
Dissection of two drug-targeted regions of Hepatitis C virus subtype 4a infecting Egyptian patients. Virus Genes (IF 1.991) Pub Date : 2020-06-22 Radwa R El-Tahan,Ahmed M Ghoneim,Hosam Zaghloul
Recently, treatment of HCV infection has been improved after the development of direct acting antivirals (DAAs) which target different viral proteins (NS3-4A, NS5A and NS5B). The activity and effectiveness of these DAAs are affected by the presence of resistance associated substitutions (RASs). This study aimed to characterize HCV genotypes circulating among Egyptian HCV patients, to dissect the full
-
First molecular characterization of avian paramyxovirus-1 (Newcastle disease virus) in Botswana. Virus Genes (IF 1.991) Pub Date : 2020-06-20 Tebogo Kgotlele,Boitumelo Modise,John Fredrick Nyange,Carter Thanda,Giovanni Cattoli,William G Dundon
Avian paramyxovirus-1 (APMV-1), the causative agent of Newcastle disease (ND) in domestic and wild avian species, has recently been reported and characterized in five southern African countries (i.e. Mozambique, Namibia, South Africa, Zambia and Zimbabwe). Since APMV-1s have never been characterized in Botswana, this study was undertaken to determine the genotype circulating in the country. Fourteen
-
Analysis of HBsAg mutations in the 25 years after the implementation of the hepatitis B vaccination plan in China. Virus Genes (IF 1.991) Pub Date : 2020-06-15 Huiming Ye,Jing Teng,Zhiyuan Lin,Ye Wang,Xiaochun Fu
Since 1992, China has promoted hepatitis B vaccination. Concurrently, during this period, increasing use of immunoglobulins and nucleoside analogues might have exerted selective pressure on the hepatitis B virus (HBV) S gene, driving mutations in the HBsAg and changed the subtype. Using the National Center for Biotechnology Information database, we obtained gene sequence information for HBV strains
-
Rapid decline of Zika virus NS1 antigen-specific antibody responses, northeastern Brazil. Virus Genes (IF 1.991) Pub Date : 2020-06-15 Andres Moreira-Soto,Gilmara de Souza Sampaio,Célia Pedroso,Ignacio Postigo-Hidalgo,Beatrice Sarah Berneck,Sebastian Ulbert,Carlos Brites,Eduardo Martins Netto,Jan Felix Drexler
Zika virus (ZIKV) is a positive-stranded RNA virus within the Flaviviridae family. After decades of circulation in Asia, ZIKV was introduced to Brazil in 2014–2015, associated with a rise in congenital malformations. Unlike the genetically related dengue virus (DENV), ZIKV constitutes only one serotype. Although assumed that ZIKV infection may engender lifelong immunity, the long-term kinetics of ZIKV
-
Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases. Virus Genes (IF 1.991) Pub Date : 2020-06-09 Miguel Ángel Freire
RNA silencing and RNA decay are functionally interlaced, regulate gene expression and play a pivotal role in antiviral responses. As a counter-defensive strategy, many plant and mammalian viruses encode suppressors which interfere with both mechanisms. However, the protein interactions that connect these pathways remain elusive. Previous work reported that RNA silencing suppressors from different potyviruses
-
Retrospective analysis of HIV-1 drug resistance mutations in Suzhou, China from 2009 to 2014. Virus Genes (IF 1.991) Pub Date : 2020-06-04 Yanhui Song,Jingping Hu,Jun He,Chunsheng Dong,Ying Yuan,Yuan Li,Ronghua Li,Xuerong Ya
In this study, we investigated drug resistance levels in human immunodeficiency virus (HIV)-1-infected patients in Suzhou by retrospectively analyzing this property and the characteristics of circulating HIV-1 strains collected from 2009 to 2014. A total of 261 HIV-1-positive plasma samples, confirmed by the Suzhou CDC, were collected and evaluated to detect HIV-1 drug resistance genotypes using an
-
Genetic and antigenic characterization of influenza A/H5N1 viruses isolated from patients in Indonesia, 2008-2015. Virus Genes (IF 1.991) Pub Date : 2020-06-01 Hana A Pawestri,Arie A Nugraha,Alvin X Han,Eka Pratiwi,Edyth Parker,Mathilde Richard,Stefan van der Vliet,Ron A M Fouchier,David H Muljono,Menno D de Jong,Vivi Setiawaty,Dirk Eggink
Since the initial detection in 2003, Indonesia has reported 200 human cases of highly pathogenic avian influenza H5N1 (HPAI H5N1), associated with an exceptionally high case fatality rate (84%) compared to other geographical regions affected by other genetic clades of the virus. However, there is limited information on the genetic diversity of HPAI H5N1 viruses, especially those isolated from humans
-
Phylodynamics of G4P[8] and G2P[4] strains of rotavirus A isolated in Russia in 2017 based on full-genome analyses. Virus Genes (IF 1.991) Pub Date : 2020-05-29 Olga V Morozova,Anna E Alekseeva,Tatiana A Sashina,Nina F Brusnigina,Natalia V Epifanova,Alexander U Kashnikov,Vladimir V Zverev,Nadezhda A Novikova
Rotavirus A is a dynamically evolving pathogen causing acute gastroenteritis in children during the first years of life. In the present study, we conducted a phylodynamic analysis based on the complete sequences of 11 segments of rotaviruses with the G4P[8] and G2P[4] genotypes isolated in Russia in 2017. Since rotavirus has a segmented genome, our analysis was performed using the Bayesian approach
-
Molecular characterization of non-polio enteroviruses isolated from children with acute flaccid paralysis in IRAN, 2015-2018. Virus Genes (IF 1.991) Pub Date : 2020-05-25 Ahmad Nejati,Seyed Mohsen Zahraei,Sussan Mahmoudi,Maryam Yousefi,Yaghoob Mollaei-Kandelous,Hamideh Tabatabaie,Najmeh Parhizgari,Parastoo Soheili,Soodeh Yousefipoor,Rakhshandeh Nategh,Shohreh Shahmahmoodi
In addition to polioviruses, non-polio enteroviruses (NPEVs) are frequently isolated from patients with acute flaccid paralysis (AFP) worldwide. In polio-free countries, there have been expectations that with disappearing wild poliovirus from the community, the rate of AFP would decrease, but the increasing number of AFP cases proved this notion to be wrong. There are speculations that NPEVs might
-
The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii. Virus Genes (IF 1.991) Pub Date : 2020-05-25 Mengjun Cheng,Man Luo,Hengyu Xi,Yunze Zhao,Shuai Le,Li-Kuang Chen,Demeng Tan,Yuan Guan,Tianqi Wang,Wenyu Han,Nannan Wu,Tongyu Zhu,Jingmin Gu
Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised
-
Genomic diversity and recombination of Seneca Valley viruses emerged in pig herds in Guangdong Province during 2019. Virus Genes (IF 1.991) Pub Date : 2020-05-23 Jianxin Liu,Qianju Guo,Huizi Li,Xianglong Yu,Boyang Liu,Bingqian Zhao,Zhangyong Ning
Seneca Valley virus (SVV) is an emerging global picornavirus that causes porcine idiopathic vesicular disease. We characterized the genome and conducted evolutionary and recombination analyses of four newly identified SVV strains which were CH-GDZS-2019, CH-GDMZ-2019, CH-GDHZ01-2019, and CH-GDHZ02-2019. Sequence alignment and phylogenetic analysis showed that strains circulating in swine herds in China
-
Analysis of viruses present in urine from patients with interstitial cystitis. Virus Genes (IF 1.991) Pub Date : 2020-05-23 Maria Teresa Sáenz Robles,Paul G Cantalupo,Alexis M Duray,Melissa Freeland,Michelle Murkowski,Adrie van Bokhoven,Alisa J Stephens-Shields,James M Pipas,Michael J Imperiale
The question of whether some cases of interstitial cystitis may have an infectious etiology has been debated for some time. Previous studies have looked for the presence of certain specific viruses, but generally did not use the types of sensitive and unbiased approaches that are currently available. As part of the MAPP (Multidisciplinary Approach to the Study of Chronic Pelvic Pain) Research Network
-
Genetic and antigenic characterization of H5 and H7 avian influenza viruses isolated from migratory waterfowl in Mongolia from 2017 to 2019. Virus Genes (IF 1.991) Pub Date : 2020-05-19 Ankhanbaatar Ulaankhuu,Enkhbold Bazarragchaa,Masatoshi Okamatsu,Takahiro Hiono,Khishgee Bodisaikhan,Tsolmon Amartuvshin,Jargalsaikhan Tserenjav,Tsogtbaatar Urangoo,Khanui Buyantogtokh,Keita Matsuno,Takanari Hattori,Tatsunari Kondoh,Masahiro Sato,Yoshihiro Takadate,Shiho Torii,Mao Isono,Kosuke Okuya,Takeshi Saito,Nodoka Kasajima,Yurie Kida,Junki Maruyama,Manabu Igarashi,Ayato Takada,Hiroshi Kida,Damdinjav
The circulation of highly pathogenic avian influenza viruses (HPAIVs) of various subtypes (e.g., H5N1, H5N6, H5N8, and H7N9) in poultry remains a global concern for animal and public health. Migratory waterfowls play important roles in the transmission of these viruses across countries. To monitor virus spread by wild birds, active surveillance for avian influenza in migratory waterfowl was conducted
-
Viruses of endophytic and pathogenic forest fungi. Virus Genes (IF 1.991) Pub Date : 2020-05-09 Abu Bakar Siddique
Mycoviruses, just as the fungal endophytes they infect, are ubiquitous biological entities on Earth. Mycoviruses constitute a diverse group of viruses, and metagenomic approaches have-through recent discoveries of been mycoviruses-only recently began to provide evidence of this astonishing diversity. The current review presents (1) various mycoviruses which infect fungal endophytes and forest pathogens
-
Effects of gE/gI deletions on the miRNA expression of PRV-infected PK-15 cells. Virus Genes (IF 1.991) Pub Date : 2020-05-08 Xiao Liu,Yuancheng Zhou,Yuan Luo,Yanxi Chen
Pseudorabies virus (PRV) belongs to the Alphaherpesvirinae subfamily of Herpesviridae. PRV-induced pseudorabies is a highly contagious disease that has caused huge economic losses to the global swine industry. The PRV gE/gI gene deletion vaccine strain (Fa ΔgE/gI strain) constructed from the PRV Fa wild-type strain was shown to have a protective effect against infection. However, the interaction between
-
A potent enzybiotic against methicillin-resistant Staphylococcus aureus. Virus Genes (IF 1.991) Pub Date : 2020-05-04 Jasjeet Kaur,Prashant Singh,Deepak Sharma,Kusum Harjai,Sanjay Chhibber
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls for the development of alternative and effective modalities against MDR S. aureus. Endolysins are bacteriophage-derived
-
Effect of viral DNA methylation on expression of hepatitis B virus proteins depends on the virus genotype. Virus Genes (IF 1.991) Pub Date : 2020-05-02 Takuya Nakamura,Jun Inoue,Masashi Ninomiya,Eiji Kakazu,Tomoaki Iwata,Satoshi Takai,Akitoshi Sano,Takayuki Kogure,Tooru Shimosegawa,Atsushi Masamune
The frequency of HBV genomic methylation in the liver was reported to vary among patients, but the detailed mechanism is still unknown. In this study, the effects of HBV genome methylation on HBV replication were investigated in vitro. A total of 6 plasmids containing 1.24-fold the HBV genome of different genotypes (subgenotypes A1, A2, B1, and C2) were purified after in vitro methylation with CpG
-